CYP11A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CYP11A1
Entrez Gene ID	1583
Full Name	cytochrome P450 family 11 subfamily A member 1
Synonyms	CYP11A,CYPXIA1,P450SCC
General protein information	Preferred Names cytochrome P450 family 11 subfamily A member 1 Names cholesterol side-chain cleavage enzyme, mitochondrial cholesterol 20-22 desmolase cholesterol monooxygenase (side-chain cleaving) cytochrome P450 11A1 cytochrome P450 family 11 subfamily A polypeptide 1 cytochrome P450(scc) cytochrome P450, subfamily XIA (cholesterol side chain cleavage) steroid 20-22-lyase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q24.1
Summary	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008].
Disorder MIM:	118485
Disorder Html:	Adrenal insufficiency, congenital, with 46XY sex reversal, partial or complete, 613743 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001099773.1	NP_001093243.1	cholesterol side-chain cleavage enzyme, mitochondrial isoform b
NM_000781.2	NP_000772.2	cholesterol side-chain cleavage enzyme, mitochondrial isoform a precursor
NM_000781.3	NP_000772.2	cholesterol side-chain cleavage enzyme, mitochondrial isoform a precursor
NM_001099773.2	NP_001093243.1	cholesterol side-chain cleavage enzyme, mitochondrial isoform b

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CYP11A1	XP_001164690.3
Canis lupus familiaris (dog)	CYP11A1	XP_535539.2
Danio rerio (zebrafish)	cyp11a1	NP_694485.2
Rattus norvegicus (Norway rat)	Cyp11a1	NP_058982.1
Danio rerio (zebrafish)	LOC563363	XP_691817.1
Mus musculus (house mouse)	Cyp11a1	NP_062753.3
Gallus gallus (chicken)	CYP11A1	NP_001001756.1
Xenopus tropicalis (tropical clawed frog)	LOC100495360	XP_002934608.1
Homo sapiens (human)	CYP11A1	NP_000772.2
Bos taurus (cattle)	CYP11A1	NP_788817.1

Gene Ontology
Bibliography

Related articles in PubMed

Active Site Structures of CYP11A1 in the Presence of Its Physiological Substrates and Alterations upon Binding of Adrenodoxin.
Zhu Q, Mak PJ, Tuckey RC, Kincaid JR
Biochemistry56(43)5786-5797(2017 10)

Genetic Polymorphisms in Estrogen Metabolic Pathway Associated with Risks of Alzheimer's Disease: Evidence from a Southern Chinese Population.
Chen LH, Fan YH, Kao PY, Ho DT, Ha JC, Chu LW, Song YQ
Journal of the American Geriatrics Society65(2)332-339(2017 Feb)

Deregulation of RUNX2 by miR-320a deficiency impairs steroidogenesis in cumulus granulosa cells from polycystic ovary syndrome (PCOS) patients.
Zhang CL, Wang H, Yan CY, Gao XF, Ling XJ
Biochemical and biophysical research communications482(4)1469-1476(2017 Jan)

Immunohistochemical analysis of steroidogenic enzymes in ovarian-type stroma of pancreatic mucinous cystic neoplasms: Comparative study of subepithelial stromal cells in intraductal papillary mucinous neoplasms of the pancreas.
Ishida K, Sasano H, Moriya T, Takahashi Y, Sugimoto R, Mue Y, Murakami K, Fujishima F, Nakamura Y, Morikawa T, Motoi F, Suzuki T, Unno M, Sugai T
Pathology international66(5)281-7(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Active Site Structures of CYP11A1 in the Presence of Its Physiological Substrates and Alterations upon Binding of Adrenodoxin
Title: Active Site Structures of CYP11A1 in the Presence of Its Physiological Substrates and Alterations upon Binding of Adrenodoxin.

The present study systematically evaluated the genetic effect of the estrogen metabolic pathway on alzheimer's based on 78 polymorphisms distributed across four genes. In the southern Chinese population, strong evidence of associations with AD were detected for polymorphisms in ESR2 and CYP11A1.
Title: Genetic Polymorphisms in Estrogen Metabolic Pathway Associated with Risks of Alzheimer's Disease: Evidence from a Southern Chinese Population.

deregulation of miR-320a/RUNX2/CYP11A1 (CYP19A1) cascade plays an important role in the development of estrogen deficiency in human cumulus granulosa cells
Title: Deregulation of RUNX2 by miR-320a deficiency impairs steroidogenesis in cumulus granulosa cells from polycystic ovary syndrome (PCOS) patients.

The steroidogenic enzymes cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc), cytochrome P450 17 alpha-hydroxylase (P450c17) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD) showed immunoreactivity in 9/20 (45.0 %), 15/20 (75.0 %) and 13/20 (65.0 %), respectively, of ovarian-type stroma from pancreatic mucinous cystic neoplasm cases.
Title: Immunohistochemical analysis of steroidogenic enzymes in ovarian-type stroma of pancreatic mucinous cystic neoplasms: Comparative study of subepithelial stromal cells in intraductal papillary mucinous neoplasms of the pancreas.

An epistatic effect between CYP11A1 and VDR polymorphisms may contribute to the predisposition to childhood asthma.
Title: 1,25D3 prevents CD8(+)Tc2 skewing and asthma development through VDR binding changes to the Cyp11a1 promoter.

The following CYP11A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP11A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08874	NM_001099773.2 Latest version!	Homo sapiens cytochrome P450 family 11 subfamily A member 1 (CYP11A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu18602	NM_000781.3 Latest version!	Homo sapiens cytochrome P450 family 11 subfamily A member 1 (CYP11A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu18602	NM_000781.2	Homo sapiens cytochrome P450 family 11 subfamily A member 1 (CYP11A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu08874	NM_001099773.1	Homo sapiens cytochrome P450 family 11 subfamily A member 1 (CYP11A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CYP11A1 ( NM_001099773.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001093243.1 Homo sapiens cytochrome P450 family 11 subfamily A member 1 (CYP11A1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08874
Clone ID Related Accession (Same CDS sequence)	NM_001099773.1 , NM_001099773.2
Accession Version	NM_001099773.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	cholesterol side-chain cleavage enzyme, mitochondrial isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA010417.1, BC032329.1 and AI149078.1. Transcript Variant: This variant (2) uses an alternate first exon compared to variant 1, resulting in the translation start site being located at a downstream AUG. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK056794.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162841, SAMN03267755 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCCAG AGGCCACCAA GAACTTTTTG CCCCTGTTGG ATGCAGTGTC TCGGGACTTC 
GTCAGTGTCC TGCACAGGCG CATCAAGAAG GCGGGCTCCG GAAATTACTC GGGGGACATC 
AGTGATGACC TGTTCCGCTT TGCCTTTGAG TCCATCACTA ACGTCATTTT TGGGGAGCGC 
CAGGGGATGC TGGAGGAAGT AGTGAACCCC GAGGCCCAGC GATTCATTGA TGCCATCTAC 
CAGATGTTCC ACACCAGCGT CCCCATGCTC AACCTTCCCC CAGACCTGTT CCGTCTGTTC 
AGGACCAAGA CCTGGAAGGA CCATGTGGCT GCATGGGACG TGATTTTCAG TAAAGCTGAC 
ATATACACCC AGAACTTCTA CTGGGAATTG AGACAGAAAG GAAGTGTTCA CCACGATTAC 
CGTGGCATCC TCTACAGACT CCTGGGAGAC AGCAAGATGT CCTTCGAGGA CATCAAGGCC 
AACGTCACAG AGATGCTGGC AGGAGGGGTG GACACGACGT CCATGACCCT GCAGTGGCAC 
TTGTATGAGA TGGCACGCAA CCTGAAGGTG CAGGATATGC TGCGGGCAGA GGTCTTGGCT 
GCGCGGCACC AGGCCCAGGG AGACATGGCC ACGATGCTAC AGCTGGTCCC CCTCCTCAAA 
GCCAGCATCA AGGAGACACT AAGACTTCAC CCCATCTCCG TGACCCTGCA GAGATATCTT 
GTAAATGACT TGGTTCTTCG AGATTACATG ATTCCTGCCA AGACACTGGT GCAAGTGGCC 
ATCTATGCTC TGGGCCGAGA GCCCACCTTC TTCTTCGACC CGGAAAATTT TGACCCAACC 
CGATGGCTGA GCAAAGACAA GAACATCACC TACTTCCGGA ACTTGGGCTT TGGCTGGGGT 
GTGCGGCAGT GTCTGGGACG GCGGATCGCT GAGCTAGAGA TGACCATCTT CCTCATCAAT 
ATGCTGGAGA ACTTCAGAGT TGAAATCCAA CACCTCAGCG ATGTGGGCAC CACATTCAAC 
CTCATTCTGA TGCCTGAAAA GCCCATCTCC TTCACCTTCT GGCCCTTTAA CCAGGAAGCA 
ACCCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093243.1
CDS	704..1795
Translation	MAPEATKNFLPLLDAVSRDFVSVLHRRIKKAGSGNYSGDISDDLFRFAFESITNVIFGERQGMLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDLFRLFRTKTWKDHVAAWDVIFSKADIYTQNFYWELRQKGSVHHDYRGILYRLLGDSKMSFEDIKANVTEMLAGGVDTTSMTLQWHLYEMARNLKVQDMLRAEVLAARHQAQGDMATMLQLVPLLKASIKETLRLHPISVTLQRYLVNDLVLRDYMIPAKTLVQVAIYALGREPTFFFDPENFDPTRWLSKDKNITYFRNLGFGWGVRQCLGRRIAELEMTIFLINMLENFRVEIQHLSDVGTTFNLILMPEKPISFTFWPFNQEATQQ

Target ORF information:

RefSeq Version	NM_001099773.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 11 subfamily A member 1 (CYP11A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099773.1

ORF Insert Sequence:

ATGGCTCCAG AGGCCACCAA GAACTTTTTG CCCCTGTTGG ATGCAGTGTC TCGGGACTTC 
GTCAGTGTCC TGCACAGGCG CATCAAGAAG GCGGGCTCCG GAAATTACTC GGGGGACATC 
AGTGATGACC TGTTCCGCTT TGCCTTTGAG TCCATCACTA ACGTCATTTT TGGGGAGCGC 
CAGGGGATGC TGGAGGAAGT AGTGAACCCC GAGGCCCAGC GATTCATTGA TGCCATCTAC 
CAGATGTTCC ACACCAGCGT CCCCATGCTC AACCTTCCCC CAGACCTGTT CCGTCTGTTC 
AGGACCAAGA CCTGGAAGGA CCATGTGGCT GCATGGGACG TGATTTTCAG TAAAGCTGAC 
ATATACACCC AGAACTTCTA CTGGGAATTG AGACAGAAAG GAAGTGTTCA CCACGATTAC 
CGTGGCATCC TCTACAGACT CCTGGGAGAC AGCAAGATGT CCTTCGAGGA CATCAAGGCC 
AACGTCACAG AGATGCTGGC AGGAGGGGTG GACACGACGT CCATGACCCT GCAGTGGCAC 
TTGTATGAGA TGGCACGCAA CCTGAAGGTG CAGGATATGC TGCGGGCAGA GGTCTTGGCT 
GCGCGGCACC AGGCCCAGGG AGACATGGCC ACGATGCTAC AGCTGGTCCC CCTCCTCAAA 
GCCAGCATCA AGGAGACACT AAGACTTCAC CCCATCTCCG TGACCCTGCA GAGATATCTT 
GTAAATGACT TGGTTCTTCG AGATTACATG ATTCCTGCCA AGACACTGGT GCAAGTGGCC 
ATCTATGCTC TGGGCCGAGA GCCCACCTTC TTCTTCGACC CGGAAAATTT TGACCCAACC 
CGATGGCTGA GCAAAGACAA GAACATCACC TACTTCCGGA ACTTGGGCTT TGGCTGGGGT 
GTGCGGCAGT GTCTGGGACG GCGGATCGCT GAGCTAGAGA TGACCATCTT CCTCATCAAT 
ATGCTGGAGA ACTTCAGAGT TGAAATCCAA CACCTCAGCG ATGTGGGCAC CACATTCAAC 
CTCATTCTGA TGCCTGAAAA GCCCATCTCC TTCACCTTCT GGCCCTTTAA CCAGGAAGCA 
ACCCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP11A1 ( NM_001099773.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001093243.1 Homo sapiens cytochrome P450 family 11 subfamily A member 1 (CYP11A1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08874
Clone ID Related Accession (Same CDS sequence)	NM_001099773.1 , NM_001099773.2
Accession Version	NM_001099773.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	cholesterol side-chain cleavage enzyme, mitochondrial isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC090826.15. On May 31, 2019 this sequence version replaced NM_001099773.1. Transcript Variant: This variant (2) uses an alternate first exon compared to variant 1, resulting in the translation start site being located at a downstream AUG. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK056794.1, SRR3476690.908711.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162841, SAMN03267755 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCCAG AGGCCACCAA GAACTTTTTG CCCCTGTTGG ATGCAGTGTC TCGGGACTTC 
GTCAGTGTCC TGCACAGGCG CATCAAGAAG GCGGGCTCCG GAAATTACTC GGGGGACATC 
AGTGATGACC TGTTCCGCTT TGCCTTTGAG TCCATCACTA ACGTCATTTT TGGGGAGCGC 
CAGGGGATGC TGGAGGAAGT AGTGAACCCC GAGGCCCAGC GATTCATTGA TGCCATCTAC 
CAGATGTTCC ACACCAGCGT CCCCATGCTC AACCTTCCCC CAGACCTGTT CCGTCTGTTC 
AGGACCAAGA CCTGGAAGGA CCATGTGGCT GCATGGGACG TGATTTTCAG TAAAGCTGAC 
ATATACACCC AGAACTTCTA CTGGGAATTG AGACAGAAAG GAAGTGTTCA CCACGATTAC 
CGTGGCATCC TCTACAGACT CCTGGGAGAC AGCAAGATGT CCTTCGAGGA CATCAAGGCC 
AACGTCACAG AGATGCTGGC AGGAGGGGTG GACACGACGT CCATGACCCT GCAGTGGCAC 
TTGTATGAGA TGGCACGCAA CCTGAAGGTG CAGGATATGC TGCGGGCAGA GGTCTTGGCT 
GCGCGGCACC AGGCCCAGGG AGACATGGCC ACGATGCTAC AGCTGGTCCC CCTCCTCAAA 
GCCAGCATCA AGGAGACACT AAGACTTCAC CCCATCTCCG TGACCCTGCA GAGATATCTT 
GTAAATGACT TGGTTCTTCG AGATTACATG ATTCCTGCCA AGACACTGGT GCAAGTGGCC 
ATCTATGCTC TGGGCCGAGA GCCCACCTTC TTCTTCGACC CGGAAAATTT TGACCCAACC 
CGATGGCTGA GCAAAGACAA GAACATCACC TACTTCCGGA ACTTGGGCTT TGGCTGGGGT 
GTGCGGCAGT GTCTGGGACG GCGGATCGCT GAGCTAGAGA TGACCATCTT CCTCATCAAT 
ATGCTGGAGA ACTTCAGAGT TGAAATCCAA CACCTCAGCG ATGTGGGCAC CACATTCAAC 
CTCATTCTGA TGCCTGAAAA GCCCATCTCC TTCACCTTCT GGCCCTTTAA CCAGGAAGCA 
ACCCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093243.1
CDS	685..1776
Translation	MAPEATKNFLPLLDAVSRDFVSVLHRRIKKAGSGNYSGDISDDLFRFAFESITNVIFGERQGMLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDLFRLFRTKTWKDHVAAWDVIFSKADIYTQNFYWELRQKGSVHHDYRGILYRLLGDSKMSFEDIKANVTEMLAGGVDTTSMTLQWHLYEMARNLKVQDMLRAEVLAARHQAQGDMATMLQLVPLLKASIKETLRLHPISVTLQRYLVNDLVLRDYMIPAKTLVQVAIYALGREPTFFFDPENFDPTRWLSKDKNITYFRNLGFGWGVRQCLGRRIAELEMTIFLINMLENFRVEIQHLSDVGTTFNLILMPEKPISFTFWPFNQEATQQ

Target ORF information:

RefSeq Version	NM_001099773.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 11 subfamily A member 1 (CYP11A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099773.2

ORF Insert Sequence:

ATGGCTCCAG AGGCCACCAA GAACTTTTTG CCCCTGTTGG ATGCAGTGTC TCGGGACTTC 
GTCAGTGTCC TGCACAGGCG CATCAAGAAG GCGGGCTCCG GAAATTACTC GGGGGACATC 
AGTGATGACC TGTTCCGCTT TGCCTTTGAG TCCATCACTA ACGTCATTTT TGGGGAGCGC 
CAGGGGATGC TGGAGGAAGT AGTGAACCCC GAGGCCCAGC GATTCATTGA TGCCATCTAC 
CAGATGTTCC ACACCAGCGT CCCCATGCTC AACCTTCCCC CAGACCTGTT CCGTCTGTTC 
AGGACCAAGA CCTGGAAGGA CCATGTGGCT GCATGGGACG TGATTTTCAG TAAAGCTGAC 
ATATACACCC AGAACTTCTA CTGGGAATTG AGACAGAAAG GAAGTGTTCA CCACGATTAC 
CGTGGCATCC TCTACAGACT CCTGGGAGAC AGCAAGATGT CCTTCGAGGA CATCAAGGCC 
AACGTCACAG AGATGCTGGC AGGAGGGGTG GACACGACGT CCATGACCCT GCAGTGGCAC 
TTGTATGAGA TGGCACGCAA CCTGAAGGTG CAGGATATGC TGCGGGCAGA GGTCTTGGCT 
GCGCGGCACC AGGCCCAGGG AGACATGGCC ACGATGCTAC AGCTGGTCCC CCTCCTCAAA 
GCCAGCATCA AGGAGACACT AAGACTTCAC CCCATCTCCG TGACCCTGCA GAGATATCTT 
GTAAATGACT TGGTTCTTCG AGATTACATG ATTCCTGCCA AGACACTGGT GCAAGTGGCC 
ATCTATGCTC TGGGCCGAGA GCCCACCTTC TTCTTCGACC CGGAAAATTT TGACCCAACC 
CGATGGCTGA GCAAAGACAA GAACATCACC TACTTCCGGA ACTTGGGCTT TGGCTGGGGT 
GTGCGGCAGT GTCTGGGACG GCGGATCGCT GAGCTAGAGA TGACCATCTT CCTCATCAAT 
ATGCTGGAGA ACTTCAGAGT TGAAATCCAA CACCTCAGCG ATGTGGGCAC CACATTCAAC 
CTCATTCTGA TGCCTGAAAA GCCCATCTCC TTCACCTTCT GGCCCTTTAA CCAGGAAGCA 
ACCCAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP11A1 ( NM_000781.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000772.2 Homo sapiens cytochrome P450 family 11 subfamily A member 1 (CYP11A1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18602
Clone ID Related Accession (Same CDS sequence)	NM_000781.2 , NM_000781.3
Accession Version	NM_000781.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Homo sapiens(human)
Product	cholesterol side-chain cleavage enzyme, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX398579.2, BC032329.1 and AI149078.1. This sequence is a reference standard in the RefSeqGene project. On Jul 24, 2007 this sequence version replaced NM_000781.1. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292300.1, BC032329.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162841, SAMN03267755 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGGCCA AGGGTCTTCC CCCACGCTCA GTCCTGGTCA AAGGCTGCCA GACCTTTCTG 
AGTGCCCCCA GGGAGGGGCT GGGGCGTCTC AGGGTGCCCA CTGGCGAGGG AGCTGGCATC 
TCCACCCGCA GTCCTCGCCC CTTCAATGAG ATCCCCTCTC CTGGTGACAA TGGCTGGCTA 
AACCTGTACC ATTTCTGGAG GGAGACGGGC ACACACAAAG TCCACCTTCA CCATGTCCAG 
AATTTCCAGA AGTATGGCCC GATTTACAGG GAGAAGCTCG GCAACGTGGA GTCGGTTTAT 
GTCATCGACC CTGAAGATGT GGCCCTTCTC TTTAAGTCCG AGGGCCCCAA CCCAGAACGA 
TTCCTCATCC CGCCCTGGGT CGCCTATCAC CAGTATTACC AGAGACCCAT AGGAGTCCTG 
TTGAAGAAGT CGGCAGCCTG GAAGAAAGAC CGGGTGGCCC TGAACCAGGA GGTGATGGCT 
CCAGAGGCCA CCAAGAACTT TTTGCCCCTG TTGGATGCAG TGTCTCGGGA CTTCGTCAGT 
GTCCTGCACA GGCGCATCAA GAAGGCGGGC TCCGGAAATT ACTCGGGGGA CATCAGTGAT 
GACCTGTTCC GCTTTGCCTT TGAGTCCATC ACTAACGTCA TTTTTGGGGA GCGCCAGGGG 
ATGCTGGAGG AAGTAGTGAA CCCCGAGGCC CAGCGATTCA TTGATGCCAT CTACCAGATG 
TTCCACACCA GCGTCCCCAT GCTCAACCTT CCCCCAGACC TGTTCCGTCT GTTCAGGACC 
AAGACCTGGA AGGACCATGT GGCTGCATGG GACGTGATTT TCAGTAAAGC TGACATATAC 
ACCCAGAACT TCTACTGGGA ATTGAGACAG AAAGGAAGTG TTCACCACGA TTACCGTGGC 
ATCCTCTACA GACTCCTGGG AGACAGCAAG ATGTCCTTCG AGGACATCAA GGCCAACGTC 
ACAGAGATGC TGGCAGGAGG GGTGGACACG ACGTCCATGA CCCTGCAGTG GCACTTGTAT 
GAGATGGCAC GCAACCTGAA GGTGCAGGAT ATGCTGCGGG CAGAGGTCTT GGCTGCGCGG 
CACCAGGCCC AGGGAGACAT GGCCACGATG CTACAGCTGG TCCCCCTCCT CAAAGCCAGC 
ATCAAGGAGA CACTAAGACT TCACCCCATC TCCGTGACCC TGCAGAGATA TCTTGTAAAT 
GACTTGGTTC TTCGAGATTA CATGATTCCT GCCAAGACAC TGGTGCAAGT GGCCATCTAT 
GCTCTGGGCC GAGAGCCCAC CTTCTTCTTC GACCCGGAAA ATTTTGACCC AACCCGATGG 
CTGAGCAAAG ACAAGAACAT CACCTACTTC CGGAACTTGG GCTTTGGCTG GGGTGTGCGG 
CAGTGTCTGG GACGGCGGAT CGCTGAGCTA GAGATGACCA TCTTCCTCAT CAATATGCTG 
GAGAACTTCA GAGTTGAAAT CCAACACCTC AGCGATGTGG GCACCACATT CAACCTCATT 
CTGATGCCTG AAAAGCCCAT CTCCTTCACC TTCTGGCCCT TTAACCAGGA AGCAACCCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000772.2
CDS	156..1721
Translation	MLAKGLPPRSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAAWKKDRVALNQEVMAPEATKNFLPLLDAVSRDFVSVLHRRIKKAGSGNYSGDISDDLFRFAFESITNVIFGERQGMLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDLFRLFRTKTWKDHVAAWDVIFSKADIYTQNFYWELRQKGSVHHDYRGILYRLLGDSKMSFEDIKANVTEMLAGGVDTTSMTLQWHLYEMARNLKVQDMLRAEVLAARHQAQGDMATMLQLVPLLKASIKETLRLHPISVTLQRYLVNDLVLRDYMIPAKTLVQVAIYALGREPTFFFDPENFDPTRWLSKDKNITYFRNLGFGWGVRQCLGRRIAELEMTIFLINMLENFRVEIQHLSDVGTTFNLILMPEKPISFTFWPFNQEATQQ

Target ORF information:

RefSeq Version	NM_000781.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 11 subfamily A member 1 (CYP11A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000781.2

ORF Insert Sequence:

ATGCTGGCCA AGGGTCTTCC CCCACGCTCA GTCCTGGTCA AAGGCTGCCA GACCTTTCTG 
AGTGCCCCCA GGGAGGGGCT GGGGCGTCTC AGGGTGCCCA CTGGCGAGGG AGCTGGCATC 
TCCACCCGCA GTCCTCGCCC CTTCAATGAG ATCCCCTCTC CTGGTGACAA TGGCTGGCTA 
AACCTGTACC ATTTCTGGAG GGAGACGGGC ACACACAAAG TCCACCTTCA CCATGTCCAG 
AATTTCCAGA AGTATGGCCC GATTTACAGG GAGAAGCTCG GCAACGTGGA GTCGGTTTAT 
GTCATCGACC CTGAAGATGT GGCCCTTCTC TTTAAGTCCG AGGGCCCCAA CCCAGAACGA 
TTCCTCATCC CGCCCTGGGT CGCCTATCAC CAGTATTACC AGAGACCCAT AGGAGTCCTG 
TTGAAGAAGT CGGCAGCCTG GAAGAAAGAC CGGGTGGCCC TGAACCAGGA GGTGATGGCT 
CCAGAGGCCA CCAAGAACTT TTTGCCCCTG TTGGATGCAG TGTCTCGGGA CTTCGTCAGT 
GTCCTGCACA GGCGCATCAA GAAGGCGGGC TCCGGAAATT ACTCGGGGGA CATCAGTGAT 
GACCTGTTCC GCTTTGCCTT TGAGTCCATC ACTAACGTCA TTTTTGGGGA GCGCCAGGGG 
ATGCTGGAGG AAGTAGTGAA CCCCGAGGCC CAGCGATTCA TTGATGCCAT CTACCAGATG 
TTCCACACCA GCGTCCCCAT GCTCAACCTT CCCCCAGACC TGTTCCGTCT GTTCAGGACC 
AAGACCTGGA AGGACCATGT GGCTGCATGG GACGTGATTT TCAGTAAAGC TGACATATAC 
ACCCAGAACT TCTACTGGGA ATTGAGACAG AAAGGAAGTG TTCACCACGA TTACCGTGGC 
ATCCTCTACA GACTCCTGGG AGACAGCAAG ATGTCCTTCG AGGACATCAA GGCCAACGTC 
ACAGAGATGC TGGCAGGAGG GGTGGACACG ACGTCCATGA CCCTGCAGTG GCACTTGTAT 
GAGATGGCAC GCAACCTGAA GGTGCAGGAT ATGCTGCGGG CAGAGGTCTT GGCTGCGCGG 
CACCAGGCCC AGGGAGACAT GGCCACGATG CTACAGCTGG TCCCCCTCCT CAAAGCCAGC 
ATCAAGGAGA CACTAAGACT TCACCCCATC TCCGTGACCC TGCAGAGATA TCTTGTAAAT 
GACTTGGTTC TTCGAGATTA CATGATTCCT GCCAAGACAC TGGTGCAAGT GGCCATCTAT 
GCTCTGGGCC GAGAGCCCAC CTTCTTCTTC GACCCGGAAA ATTTTGACCC AACCCGATGG 
CTGAGCAAAG ACAAGAACAT CACCTACTTC CGGAACTTGG GCTTTGGCTG GGGTGTGCGG 
CAGTGTCTGG GACGGCGGAT CGCTGAGCTA GAGATGACCA TCTTCCTCAT CAATATGCTG 
GAGAACTTCA GAGTTGAAAT CCAACACCTC AGCGATGTGG GCACCACATT CAACCTCATT 
CTGATGCCTG AAAAGCCCAT CTCCTTCACC TTCTGGCCCT TTAACCAGGA AGCAACCCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP11A1 ( NM_000781.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000772.2 Homo sapiens cytochrome P450 family 11 subfamily A member 1 (CYP11A1), transcript variant 1, mRNA.

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CloneID	OHu18602
Clone ID Related Accession (Same CDS sequence)	NM_000781.2 , NM_000781.3
Accession Version	NM_000781.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	cholesterol side-chain cleavage enzyme, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX398579.2, BC032329.1 and AI149078.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000781.2. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292300.1, SRR5189667.171723.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162841, SAMN03267755 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000268053.11/ ENSP00000268053.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGGCCA AGGGTCTTCC CCCACGCTCA GTCCTGGTCA AAGGCTGCCA GACCTTTCTG 
AGTGCCCCCA GGGAGGGGCT GGGGCGTCTC AGGGTGCCCA CTGGCGAGGG AGCTGGCATC 
TCCACCCGCA GTCCTCGCCC CTTCAATGAG ATCCCCTCTC CTGGTGACAA TGGCTGGCTA 
AACCTGTACC ATTTCTGGAG GGAGACGGGC ACACACAAAG TCCACCTTCA CCATGTCCAG 
AATTTCCAGA AGTATGGCCC GATTTACAGG GAGAAGCTCG GCAACGTGGA GTCGGTTTAT 
GTCATCGACC CTGAAGATGT GGCCCTTCTC TTTAAGTCCG AGGGCCCCAA CCCAGAACGA 
TTCCTCATCC CGCCCTGGGT CGCCTATCAC CAGTATTACC AGAGACCCAT AGGAGTCCTG 
TTGAAGAAGT CGGCAGCCTG GAAGAAAGAC CGGGTGGCCC TGAACCAGGA GGTGATGGCT 
CCAGAGGCCA CCAAGAACTT TTTGCCCCTG TTGGATGCAG TGTCTCGGGA CTTCGTCAGT 
GTCCTGCACA GGCGCATCAA GAAGGCGGGC TCCGGAAATT ACTCGGGGGA CATCAGTGAT 
GACCTGTTCC GCTTTGCCTT TGAGTCCATC ACTAACGTCA TTTTTGGGGA GCGCCAGGGG 
ATGCTGGAGG AAGTAGTGAA CCCCGAGGCC CAGCGATTCA TTGATGCCAT CTACCAGATG 
TTCCACACCA GCGTCCCCAT GCTCAACCTT CCCCCAGACC TGTTCCGTCT GTTCAGGACC 
AAGACCTGGA AGGACCATGT GGCTGCATGG GACGTGATTT TCAGTAAAGC TGACATATAC 
ACCCAGAACT TCTACTGGGA ATTGAGACAG AAAGGAAGTG TTCACCACGA TTACCGTGGC 
ATCCTCTACA GACTCCTGGG AGACAGCAAG ATGTCCTTCG AGGACATCAA GGCCAACGTC 
ACAGAGATGC TGGCAGGAGG GGTGGACACG ACGTCCATGA CCCTGCAGTG GCACTTGTAT 
GAGATGGCAC GCAACCTGAA GGTGCAGGAT ATGCTGCGGG CAGAGGTCTT GGCTGCGCGG 
CACCAGGCCC AGGGAGACAT GGCCACGATG CTACAGCTGG TCCCCCTCCT CAAAGCCAGC 
ATCAAGGAGA CACTAAGACT TCACCCCATC TCCGTGACCC TGCAGAGATA TCTTGTAAAT 
GACTTGGTTC TTCGAGATTA CATGATTCCT GCCAAGACAC TGGTGCAAGT GGCCATCTAT 
GCTCTGGGCC GAGAGCCCAC CTTCTTCTTC GACCCGGAAA ATTTTGACCC AACCCGATGG 
CTGAGCAAAG ACAAGAACAT CACCTACTTC CGGAACTTGG GCTTTGGCTG GGGTGTGCGG 
CAGTGTCTGG GACGGCGGAT CGCTGAGCTA GAGATGACCA TCTTCCTCAT CAATATGCTG 
GAGAACTTCA GAGTTGAAAT CCAACACCTC AGCGATGTGG GCACCACATT CAACCTCATT 
CTGATGCCTG AAAAGCCCAT CTCCTTCACC TTCTGGCCCT TTAACCAGGA AGCAACCCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000772.2
CDS	62..1627
Translation	MLAKGLPPRSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAAWKKDRVALNQEVMAPEATKNFLPLLDAVSRDFVSVLHRRIKKAGSGNYSGDISDDLFRFAFESITNVIFGERQGMLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDLFRLFRTKTWKDHVAAWDVIFSKADIYTQNFYWELRQKGSVHHDYRGILYRLLGDSKMSFEDIKANVTEMLAGGVDTTSMTLQWHLYEMARNLKVQDMLRAEVLAARHQAQGDMATMLQLVPLLKASIKETLRLHPISVTLQRYLVNDLVLRDYMIPAKTLVQVAIYALGREPTFFFDPENFDPTRWLSKDKNITYFRNLGFGWGVRQCLGRRIAELEMTIFLINMLENFRVEIQHLSDVGTTFNLILMPEKPISFTFWPFNQEATQQ

Target ORF information:

RefSeq Version	NM_000781.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 11 subfamily A member 1 (CYP11A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000781.3

ORF Insert Sequence:

ATGCTGGCCA AGGGTCTTCC CCCACGCTCA GTCCTGGTCA AAGGCTGCCA GACCTTTCTG 
AGTGCCCCCA GGGAGGGGCT GGGGCGTCTC AGGGTGCCCA CTGGCGAGGG AGCTGGCATC 
TCCACCCGCA GTCCTCGCCC CTTCAATGAG ATCCCCTCTC CTGGTGACAA TGGCTGGCTA 
AACCTGTACC ATTTCTGGAG GGAGACGGGC ACACACAAAG TCCACCTTCA CCATGTCCAG 
AATTTCCAGA AGTATGGCCC GATTTACAGG GAGAAGCTCG GCAACGTGGA GTCGGTTTAT 
GTCATCGACC CTGAAGATGT GGCCCTTCTC TTTAAGTCCG AGGGCCCCAA CCCAGAACGA 
TTCCTCATCC CGCCCTGGGT CGCCTATCAC CAGTATTACC AGAGACCCAT AGGAGTCCTG 
TTGAAGAAGT CGGCAGCCTG GAAGAAAGAC CGGGTGGCCC TGAACCAGGA GGTGATGGCT 
CCAGAGGCCA CCAAGAACTT TTTGCCCCTG TTGGATGCAG TGTCTCGGGA CTTCGTCAGT 
GTCCTGCACA GGCGCATCAA GAAGGCGGGC TCCGGAAATT ACTCGGGGGA CATCAGTGAT 
GACCTGTTCC GCTTTGCCTT TGAGTCCATC ACTAACGTCA TTTTTGGGGA GCGCCAGGGG 
ATGCTGGAGG AAGTAGTGAA CCCCGAGGCC CAGCGATTCA TTGATGCCAT CTACCAGATG 
TTCCACACCA GCGTCCCCAT GCTCAACCTT CCCCCAGACC TGTTCCGTCT GTTCAGGACC 
AAGACCTGGA AGGACCATGT GGCTGCATGG GACGTGATTT TCAGTAAAGC TGACATATAC 
ACCCAGAACT TCTACTGGGA ATTGAGACAG AAAGGAAGTG TTCACCACGA TTACCGTGGC 
ATCCTCTACA GACTCCTGGG AGACAGCAAG ATGTCCTTCG AGGACATCAA GGCCAACGTC 
ACAGAGATGC TGGCAGGAGG GGTGGACACG ACGTCCATGA CCCTGCAGTG GCACTTGTAT 
GAGATGGCAC GCAACCTGAA GGTGCAGGAT ATGCTGCGGG CAGAGGTCTT GGCTGCGCGG 
CACCAGGCCC AGGGAGACAT GGCCACGATG CTACAGCTGG TCCCCCTCCT CAAAGCCAGC 
ATCAAGGAGA CACTAAGACT TCACCCCATC TCCGTGACCC TGCAGAGATA TCTTGTAAAT 
GACTTGGTTC TTCGAGATTA CATGATTCCT GCCAAGACAC TGGTGCAAGT GGCCATCTAT 
GCTCTGGGCC GAGAGCCCAC CTTCTTCTTC GACCCGGAAA ATTTTGACCC AACCCGATGG 
CTGAGCAAAG ACAAGAACAT CACCTACTTC CGGAACTTGG GCTTTGGCTG GGGTGTGCGG 
CAGTGTCTGG GACGGCGGAT CGCTGAGCTA GAGATGACCA TCTTCCTCAT CAATATGCTG 
GAGAACTTCA GAGTTGAAAT CCAACACCTC AGCGATGTGG GCACCACATT CAACCTCATT 
CTGATGCCTG AAAAGCCCAT CTCCTTCACC TTCTGGCCCT TTAACCAGGA AGCAACCCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Active Site Structures of CYP11A1 in the Presence of Its Physiological Substrates and Alterations upon Binding of Adrenodoxin.
Biochemistry56(43)5786-5797(2017 10)
Zhu Q,Mak PJ,Tuckey RC,Kincaid JR

Genetic Polymorphisms in Estrogen Metabolic Pathway Associated with Risks of Alzheimer's Disease: Evidence from a Southern Chinese Population.
Journal of the American Geriatrics Society65(2)332-339(2017 Feb)
Chen LH,Fan YH,Kao PY,Ho DT,Ha JC,Chu LW,Song YQ

Deregulation of RUNX2 by miR-320a deficiency impairs steroidogenesis in cumulus granulosa cells from polycystic ovary syndrome (PCOS) patients.
Biochemical and biophysical research communications482(4)1469-1476(2017 Jan)
Zhang CL,Wang H,Yan CY,Gao XF,Ling XJ

Immunohistochemical analysis of steroidogenic enzymes in ovarian-type stroma of pancreatic mucinous cystic neoplasms: Comparative study of subepithelial stromal cells in intraductal papillary mucinous neoplasms of the pancreas.
Pathology international66(5)281-7(2016 May)
Ishida K,Sasano H,Moriya T,Takahashi Y,Sugimoto R,Mue Y,Murakami K,Fujishima F,Nakamura Y,Morikawa T,Motoi F,Suzuki T,Unno M,Sugai T

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CYP11A1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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