GRK2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRK2
Entrez Gene ID	156
Full Name	G protein-coupled receptor kinase 2
Synonyms	ADRBK1,BARK1,BETA-ARK1
General protein information	Preferred Names G protein-coupled receptor kinase 2 Names beta-adrenergic receptor kinase 1 adrenergic beta receptor kinase 1 beta-ARK-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.2
Summary	This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017].
Disorder MIM:	109635

mRNA and Protein(s)

mRNA	Protein	Name
XM_011544773.1	XP_011543075.1	beta-adrenergic receptor kinase 1 isoform X2
NM_001619.4	NP_001610.2	beta-adrenergic receptor kinase 1
NM_001619.3	NP_001610.2	beta-adrenergic receptor kinase 1
NM_001619.5	NP_001610.2	beta-adrenergic receptor kinase 1
XM_024448376.1	XP_024304144.1	beta-adrenergic receptor kinase 1 isoform X1

Pathways from BioSystems

Homologies

	ADRBK1	XP_001117955.2
Bos taurus (cattle)	ADRBK1	NP_777135.1
Pan troglodytes (chimpanzee)	ADRBK1	XP_508589.3
Canis lupus familiaris (dog)	ADRBK1	XP_852085.1
Mus musculus (house mouse)	Adrbk1	NP_570933.1
Homo sapiens (human)	ADRBK1	NP_001610.2
Rattus norvegicus (Norway rat)	Adrbk1	NP_036908.1
Gallus gallus (chicken)	ADRBK1	NP_001026524.1

Gene Ontology
Bibliography

Related articles in PubMed

?-Arrestin2 directly or through GRK2 inhibits PKC?II activation in a ubiquitination-dependent manner.
Zhang X, Zheng M, Sun N, Kim KM
Biochimica et biophysica acta1865(1)142-157(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Low grk2 expression is associated with lung metastasis in gastric cancer.
Title: Long noncoding RNA UCA1 promotes tumour metastasis by inducing GRK2 degradation in gastric cancer.

GRK2 is negatively related to IGF1R and IGF1R, but not GRK2, was associated with the tumour-node-metastasis stage and overall and disease-free survival in hepatocellular carcinoma.
Title: Expression of GRK2 and IGF1R in hepatocellular carcinoma: clinicopathological and prognostic significance.

The tyrosine-phosphorylated GRK2 mediates this inhibition by acting on the second intracellular loop of D3R.
Title: The EGF receptor inhibits the signaling of dopamine D

Data, including data from studies in heterozygous knockout mice, suggest that GRK2 is involved in TNFalpha-induced wound healing in epithelial cells of the colon; GRK2 appears to inhibit TNFalpha-induced apoptosis; GRK2 inhibits TNFalpha-induced ERK activation by inhibiting generation of reactive oxygen species. Homozygous knockout of GRK2 is embryonically lethal in mice.
Title: G-protein-coupled receptor kinase-2 is a critical regulator of TNF? signaling in colon epithelial cells.

Our data suggest that GRK2 acts as an important onco-modulator by strengthening the functionality of key players in breast tumorigenesis such as HDAC6 and Pin1.
Title: G Protein-coupled Receptor Kinase 2 (GRK2) Promotes Breast Tumorigenesis Through a HDAC6-Pin1 Axis.

The following GRK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08965	NM_001619.5 Latest version!	Homo sapiens G protein-coupled receptor kinase 2 (GRK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu56194	XM_011544773.1 Latest version!	Homo sapiens G protein-coupled receptor kinase 2 (GRK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu106620	XM_024448376.1 Latest version!	Homo sapiens G protein-coupled receptor kinase 2 (GRK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu08965	NM_001619.3	Homo sapiens G protein-coupled receptor kinase 2 (GRK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu08965	NM_001619.4	Homo sapiens G protein-coupled receptor kinase 2 (GRK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRK2 ( NM_001619.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001610.2 Homo sapiens G protein-coupled receptor kinase 2 (GRK2), mRNA.

Price & Availability↑

CloneID	OHu08965
Clone ID Related Accession (Same CDS sequence)	NM_001619.4 , NM_001619.3 , NM_001619.5
Accession Version	NM_001619.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-12
Organism	Homo sapiens(human)
Product	beta-adrenergic receptor kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN989901.1, BC090863.1 and AI888604.1. On Sep 16, 2017 this sequence version replaced NM_001619.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC037963.1, SRR1163657.418571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACC TGGAGGCGGT GCTGGCCGAC GTGAGCTACC TGATGGCCAT GGAGAAGAGC 
AAGGCCACGC CGGCCGCGCG CGCCAGCAAG AAGATCCTGC TGCCCGAGCC CAGCATCCGC 
AGTGTCATGC AGAAGTACCT GGAGGACCGG GGCGAGGTGA CCTTTGAGAA GATCTTTTCC 
CAGAAGCTGG GGTACCTGCT CTTCCGAGAC TTCTGCCTGA ACCACCTGGA GGAGGCCAGG 
CCCTTGGTGG AATTCTATGA GGAGATCAAG AAGTACGAGA AGCTGGAGAC GGAGGAGGAG 
CGTGTGGCCC GCAGCCGGGA GATCTTCGAC TCATACATCA TGAAGGAGCT GCTGGCCTGC 
TCGCATCCCT TCTCGAAGAG TGCCACTGAG CATGTCCAAG GCCACCTGGG GAAGAAGCAG 
GTGCCTCCGG ATCTCTTCCA GCCATACATC GAAGAGATTT GTCAAAACCT CCGAGGGGAC 
GTGTTCCAGA AATTCATTGA GAGCGATAAG TTCACACGGT TTTGCCAGTG GAAGAATGTG 
GAGCTCAACA TCCACCTGAC CATGAATGAC TTCAGCGTGC ATCGCATCAT TGGGCGCGGG 
GGCTTTGGCG AGGTCTATGG GTGCCGGAAG GCTGACACAG GCAAGATGTA CGCCATGAAG 
TGCCTGGACA AAAAGCGCAT CAAGATGAAG CAGGGGGAGA CCCTGGCCCT GAACGAGCGC 
ATCATGCTCT CGCTCGTCAG CACTGGGGAC TGCCCATTCA TTGTCTGCAT GTCATACGCG 
TTCCACACGC CAGACAAGCT CAGCTTCATC CTGGACCTCA TGAACGGTGG GGACCTGCAC 
TACCACCTCT CCCAGCACGG GGTCTTCTCA GAGGCTGACA TGCGCTTCTA TGCGGCCGAG 
ATCATCCTGG GCCTGGAGCA CATGCACAAC CGCTTCGTGG TCTACCGGGA CCTGAAGCCA 
GCCAACATCC TTCTGGACGA GCATGGCCAC GTGCGGATCT CGGACCTGGG CCTGGCCTGT 
GACTTCTCCA AGAAGAAGCC CCATGCCAGC GTGGGCACCC ACGGGTACAT GGCTCCGGAG 
GTCCTGCAGA AGGGCGTGGC CTACGACAGC AGTGCCGACT GGTTCTCTCT GGGGTGCATG 
CTCTTCAAGT TGCTGCGGGG GCACAGCCCC TTCCGGCAGC ACAAGACCAA AGACAAGCAT 
GAGATCGACC GCATGACGCT GACGATGGCC GTGGAGCTGC CCGACTCCTT CTCCCCTGAA 
CTACGCTCCC TGCTGGAGGG GTTGCTGCAG AGGGATGTCA ACCGGAGATT GGGCTGCCTG 
GGCCGAGGGG CTCAGGAGGT GAAAGAGAGC CCCTTTTTCC GCTCCCTGGA CTGGCAGATG 
GTCTTCTTGC AGAAGTACCC TCCCCCGCTG ATCCCCCCAC GAGGGGAGGT GAACGCGGCC 
GACGCCTTCG ACATTGGCTC CTTCGATGAG GAGGACACAA AAGGAATCAA GTTACTGGAC 
AGTGATCAGG AGCTCTACCG CAACTTCCCC CTCACCATCT CGGAGCGGTG GCAGCAGGAG 
GTGGCAGAGA CTGTCTTCGA CACCATCAAC GCTGAGACAG ACCGGCTGGA GGCTCGCAAG 
AAAGCCAAGA ACAAGCAGCT GGGCCATGAG GAAGACTACG CCCTGGGCAA GGACTGCATC 
ATGCATGGCT ACATGTCCAA GATGGGCAAC CCCTTCCTGA CCCAGTGGCA GCGGCGGTAC 
TTCTACCTGT TCCCCAACCG CCTCGAGTGG CGGGGCGAGG GCGAGGCCCC GCAGAGCCTG 
CTGACCATGG AGGAGATCCA GTCGGTGGAG GAGACGCAGA TCAAGGAGCG CAAGTGCCTG 
CTCCTCAAGA TCCGCGGTGG GAAACAGTTC ATTTTGCAGT GCGATAGCGA CCCTGAGCTG 
GTGCAGTGGA AGAAGGAGCT GCGCGACGCC TACCGCGAGG CCCAGCAGCT GGTGCAGCGG 
GTGCCCAAGA TGAAGAACAA GCCGCGCTCG CCCGTGGTGG AGCTGAGCAA GGTGCCGCTG 
GTCCAGCGCG GCAGTGCCAA CGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001610.2
CDS	301..2370
Translation	MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLACSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL

Target ORF information:

RefSeq Version	NM_001619.4
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor kinase 2 (GRK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001619.4

ORF Insert Sequence:

ATGGCGGACC TGGAGGCGGT GCTGGCCGAC GTGAGCTACC TGATGGCCAT GGAGAAGAGC 
AAGGCCACGC CGGCCGCGCG CGCCAGCAAG AAGATCCTGC TGCCCGAGCC CAGCATCCGC 
AGTGTCATGC AGAAGTACCT GGAGGACCGG GGCGAGGTGA CCTTTGAGAA GATCTTTTCC 
CAGAAGCTGG GGTACCTGCT CTTCCGAGAC TTCTGCCTGA ACCACCTGGA GGAGGCCAGG 
CCCTTGGTGG AATTCTATGA GGAGATCAAG AAGTACGAGA AGCTGGAGAC GGAGGAGGAG 
CGTGTGGCCC GCAGCCGGGA GATCTTCGAC TCATACATCA TGAAGGAGCT GCTGGCCTGC 
TCGCATCCCT TCTCGAAGAG TGCCACTGAG CATGTCCAAG GCCACCTGGG GAAGAAGCAG 
GTGCCTCCGG ATCTCTTCCA GCCATACATC GAAGAGATTT GTCAAAACCT CCGAGGGGAC 
GTGTTCCAGA AATTCATTGA GAGCGATAAG TTCACACGGT TTTGCCAGTG GAAGAATGTG 
GAGCTCAACA TCCACCTGAC CATGAATGAC TTCAGCGTGC ATCGCATCAT TGGGCGCGGG 
GGCTTTGGCG AGGTCTATGG GTGCCGGAAG GCTGACACAG GCAAGATGTA CGCCATGAAG 
TGCCTGGACA AAAAGCGCAT CAAGATGAAG CAGGGGGAGA CCCTGGCCCT GAACGAGCGC 
ATCATGCTCT CGCTCGTCAG CACTGGGGAC TGCCCATTCA TTGTCTGCAT GTCATACGCG 
TTCCACACGC CAGACAAGCT CAGCTTCATC CTGGACCTCA TGAACGGTGG GGACCTGCAC 
TACCACCTCT CCCAGCACGG GGTCTTCTCA GAGGCTGACA TGCGCTTCTA TGCGGCCGAG 
ATCATCCTGG GCCTGGAGCA CATGCACAAC CGCTTCGTGG TCTACCGGGA CCTGAAGCCA 
GCCAACATCC TTCTGGACGA GCATGGCCAC GTGCGGATCT CGGACCTGGG CCTGGCCTGT 
GACTTCTCCA AGAAGAAGCC CCATGCCAGC GTGGGCACCC ACGGGTACAT GGCTCCGGAG 
GTCCTGCAGA AGGGCGTGGC CTACGACAGC AGTGCCGACT GGTTCTCTCT GGGGTGCATG 
CTCTTCAAGT TGCTGCGGGG GCACAGCCCC TTCCGGCAGC ACAAGACCAA AGACAAGCAT 
GAGATCGACC GCATGACGCT GACGATGGCC GTGGAGCTGC CCGACTCCTT CTCCCCTGAA 
CTACGCTCCC TGCTGGAGGG GTTGCTGCAG AGGGATGTCA ACCGGAGATT GGGCTGCCTG 
GGCCGAGGGG CTCAGGAGGT GAAAGAGAGC CCCTTTTTCC GCTCCCTGGA CTGGCAGATG 
GTCTTCTTGC AGAAGTACCC TCCCCCGCTG ATCCCCCCAC GAGGGGAGGT GAACGCGGCC 
GACGCCTTCG ACATTGGCTC CTTCGATGAG GAGGACACAA AAGGAATCAA GTTACTGGAC 
AGTGATCAGG AGCTCTACCG CAACTTCCCC CTCACCATCT CGGAGCGGTG GCAGCAGGAG 
GTGGCAGAGA CTGTCTTCGA CACCATCAAC GCTGAGACAG ACCGGCTGGA GGCTCGCAAG 
AAAGCCAAGA ACAAGCAGCT GGGCCATGAG GAAGACTACG CCCTGGGCAA GGACTGCATC 
ATGCATGGCT ACATGTCCAA GATGGGCAAC CCCTTCCTGA CCCAGTGGCA GCGGCGGTAC 
TTCTACCTGT TCCCCAACCG CCTCGAGTGG CGGGGCGAGG GCGAGGCCCC GCAGAGCCTG 
CTGACCATGG AGGAGATCCA GTCGGTGGAG GAGACGCAGA TCAAGGAGCG CAAGTGCCTG 
CTCCTCAAGA TCCGCGGTGG GAAACAGTTC ATTTTGCAGT GCGATAGCGA CCCTGAGCTG 
GTGCAGTGGA AGAAGGAGCT GCGCGACGCC TACCGCGAGG CCCAGCAGCT GGTGCAGCGG 
GTGCCCAAGA TGAAGAACAA GCCGCGCTCG CCCGTGGTGG AGCTGAGCAA GGTGCCGCTG 
GTCCAGCGCG GCAGTGCCAA CGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK2 ( NM_001619.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001610.2 Homo sapiens G protein-coupled receptor kinase 2 (GRK2), mRNA.

Price & Availability↑

CloneID	OHu08965
Clone ID Related Accession (Same CDS sequence)	NM_001619.4 , NM_001619.3 , NM_001619.5
Accession Version	NM_001619.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-05
Organism	Homo sapiens(Human)
Product	beta-adrenergic receptor kinase 1
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN989901.1, BC090863.1 and AI888604.1. On Jun 2, 2007 this sequence version replaced NM_001619.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC037963.1, SRR1163657.418571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACC TGGAGGCGGT GCTGGCCGAC GTGAGCTACC TGATGGCCAT GGAGAAGAGC 
AAGGCCACGC CGGCCGCGCG CGCCAGCAAG AAGATCCTGC TGCCCGAGCC CAGCATCCGC 
AGTGTCATGC AGAAGTACCT GGAGGACCGG GGCGAGGTGA CCTTTGAGAA GATCTTTTCC 
CAGAAGCTGG GGTACCTGCT CTTCCGAGAC TTCTGCCTGA ACCACCTGGA GGAGGCCAGG 
CCCTTGGTGG AATTCTATGA GGAGATCAAG AAGTACGAGA AGCTGGAGAC GGAGGAGGAG 
CGTGTGGCCC GCAGCCGGGA GATCTTCGAC TCATACATCA TGAAGGAGCT GCTGGCCTGC 
TCGCATCCCT TCTCGAAGAG TGCCACTGAG CATGTCCAAG GCCACCTGGG GAAGAAGCAG 
GTGCCTCCGG ATCTCTTCCA GCCATACATC GAAGAGATTT GTCAAAACCT CCGAGGGGAC 
GTGTTCCAGA AATTCATTGA GAGCGATAAG TTCACACGGT TTTGCCAGTG GAAGAATGTG 
GAGCTCAACA TCCACCTGAC CATGAATGAC TTCAGCGTGC ATCGCATCAT TGGGCGCGGG 
GGCTTTGGCG AGGTCTATGG GTGCCGGAAG GCTGACACAG GCAAGATGTA CGCCATGAAG 
TGCCTGGACA AAAAGCGCAT CAAGATGAAG CAGGGGGAGA CCCTGGCCCT GAACGAGCGC 
ATCATGCTCT CGCTCGTCAG CACTGGGGAC TGCCCATTCA TTGTCTGCAT GTCATACGCG 
TTCCACACGC CAGACAAGCT CAGCTTCATC CTGGACCTCA TGAACGGTGG GGACCTGCAC 
TACCACCTCT CCCAGCACGG GGTCTTCTCA GAGGCTGACA TGCGCTTCTA TGCGGCCGAG 
ATCATCCTGG GCCTGGAGCA CATGCACAAC CGCTTCGTGG TCTACCGGGA CCTGAAGCCA 
GCCAACATCC TTCTGGACGA GCATGGCCAC GTGCGGATCT CGGACCTGGG CCTGGCCTGT 
GACTTCTCCA AGAAGAAGCC CCATGCCAGC GTGGGCACCC ACGGGTACAT GGCTCCGGAG 
GTCCTGCAGA AGGGCGTGGC CTACGACAGC AGTGCCGACT GGTTCTCTCT GGGGTGCATG 
CTCTTCAAGT TGCTGCGGGG GCACAGCCCC TTCCGGCAGC ACAAGACCAA AGACAAGCAT 
GAGATCGACC GCATGACGCT GACGATGGCC GTGGAGCTGC CCGACTCCTT CTCCCCTGAA 
CTACGCTCCC TGCTGGAGGG GTTGCTGCAG AGGGATGTCA ACCGGAGATT GGGCTGCCTG 
GGCCGAGGGG CTCAGGAGGT GAAAGAGAGC CCCTTTTTCC GCTCCCTGGA CTGGCAGATG 
GTCTTCTTGC AGAAGTACCC TCCCCCGCTG ATCCCCCCAC GAGGGGAGGT GAACGCGGCC 
GACGCCTTCG ACATTGGCTC CTTCGATGAG GAGGACACAA AAGGAATCAA GTTACTGGAC 
AGTGATCAGG AGCTCTACCG CAACTTCCCC CTCACCATCT CGGAGCGGTG GCAGCAGGAG 
GTGGCAGAGA CTGTCTTCGA CACCATCAAC GCTGAGACAG ACCGGCTGGA GGCTCGCAAG 
AAAGCCAAGA ACAAGCAGCT GGGCCATGAG GAAGACTACG CCCTGGGCAA GGACTGCATC 
ATGCATGGCT ACATGTCCAA GATGGGCAAC CCCTTCCTGA CCCAGTGGCA GCGGCGGTAC 
TTCTACCTGT TCCCCAACCG CCTCGAGTGG CGGGGCGAGG GCGAGGCCCC GCAGAGCCTG 
CTGACCATGG AGGAGATCCA GTCGGTGGAG GAGACGCAGA TCAAGGAGCG CAAGTGCCTG 
CTCCTCAAGA TCCGCGGTGG GAAACAGTTC ATTTTGCAGT GCGATAGCGA CCCTGAGCTG 
GTGCAGTGGA AGAAGGAGCT GCGCGACGCC TACCGCGAGG CCCAGCAGCT GGTGCAGCGG 
GTGCCCAAGA TGAAGAACAA GCCGCGCTCG CCCGTGGTGG AGCTGAGCAA GGTGCCGCTG 
GTCCAGCGCG GCAGTGCCAA CGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001610.2
CDS	267..2336
Translation	MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLACSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL

Target ORF information:

RefSeq Version	NM_001619.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens G protein-coupled receptor kinase 2 (GRK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001619.3

ORF Insert Sequence:

ATGGCGGACC TGGAGGCGGT GCTGGCCGAC GTGAGCTACC TGATGGCCAT GGAGAAGAGC 
AAGGCCACGC CGGCCGCGCG CGCCAGCAAG AAGATCCTGC TGCCCGAGCC CAGCATCCGC 
AGTGTCATGC AGAAGTACCT GGAGGACCGG GGCGAGGTGA CCTTTGAGAA GATCTTTTCC 
CAGAAGCTGG GGTACCTGCT CTTCCGAGAC TTCTGCCTGA ACCACCTGGA GGAGGCCAGG 
CCCTTGGTGG AATTCTATGA GGAGATCAAG AAGTACGAGA AGCTGGAGAC GGAGGAGGAG 
CGTGTGGCCC GCAGCCGGGA GATCTTCGAC TCATACATCA TGAAGGAGCT GCTGGCCTGC 
TCGCATCCCT TCTCGAAGAG TGCCACTGAG CATGTCCAAG GCCACCTGGG GAAGAAGCAG 
GTGCCTCCGG ATCTCTTCCA GCCATACATC GAAGAGATTT GTCAAAACCT CCGAGGGGAC 
GTGTTCCAGA AATTCATTGA GAGCGATAAG TTCACACGGT TTTGCCAGTG GAAGAATGTG 
GAGCTCAACA TCCACCTGAC CATGAATGAC TTCAGCGTGC ATCGCATCAT TGGGCGCGGG 
GGCTTTGGCG AGGTCTATGG GTGCCGGAAG GCTGACACAG GCAAGATGTA CGCCATGAAG 
TGCCTGGACA AAAAGCGCAT CAAGATGAAG CAGGGGGAGA CCCTGGCCCT GAACGAGCGC 
ATCATGCTCT CGCTCGTCAG CACTGGGGAC TGCCCATTCA TTGTCTGCAT GTCATACGCG 
TTCCACACGC CAGACAAGCT CAGCTTCATC CTGGACCTCA TGAACGGTGG GGACCTGCAC 
TACCACCTCT CCCAGCACGG GGTCTTCTCA GAGGCTGACA TGCGCTTCTA TGCGGCCGAG 
ATCATCCTGG GCCTGGAGCA CATGCACAAC CGCTTCGTGG TCTACCGGGA CCTGAAGCCA 
GCCAACATCC TTCTGGACGA GCATGGCCAC GTGCGGATCT CGGACCTGGG CCTGGCCTGT 
GACTTCTCCA AGAAGAAGCC CCATGCCAGC GTGGGCACCC ACGGGTACAT GGCTCCGGAG 
GTCCTGCAGA AGGGCGTGGC CTACGACAGC AGTGCCGACT GGTTCTCTCT GGGGTGCATG 
CTCTTCAAGT TGCTGCGGGG GCACAGCCCC TTCCGGCAGC ACAAGACCAA AGACAAGCAT 
GAGATCGACC GCATGACGCT GACGATGGCC GTGGAGCTGC CCGACTCCTT CTCCCCTGAA 
CTACGCTCCC TGCTGGAGGG GTTGCTGCAG AGGGATGTCA ACCGGAGATT GGGCTGCCTG 
GGCCGAGGGG CTCAGGAGGT GAAAGAGAGC CCCTTTTTCC GCTCCCTGGA CTGGCAGATG 
GTCTTCTTGC AGAAGTACCC TCCCCCGCTG ATCCCCCCAC GAGGGGAGGT GAACGCGGCC 
GACGCCTTCG ACATTGGCTC CTTCGATGAG GAGGACACAA AAGGAATCAA GTTACTGGAC 
AGTGATCAGG AGCTCTACCG CAACTTCCCC CTCACCATCT CGGAGCGGTG GCAGCAGGAG 
GTGGCAGAGA CTGTCTTCGA CACCATCAAC GCTGAGACAG ACCGGCTGGA GGCTCGCAAG 
AAAGCCAAGA ACAAGCAGCT GGGCCATGAG GAAGACTACG CCCTGGGCAA GGACTGCATC 
ATGCATGGCT ACATGTCCAA GATGGGCAAC CCCTTCCTGA CCCAGTGGCA GCGGCGGTAC 
TTCTACCTGT TCCCCAACCG CCTCGAGTGG CGGGGCGAGG GCGAGGCCCC GCAGAGCCTG 
CTGACCATGG AGGAGATCCA GTCGGTGGAG GAGACGCAGA TCAAGGAGCG CAAGTGCCTG 
CTCCTCAAGA TCCGCGGTGG GAAACAGTTC ATTTTGCAGT GCGATAGCGA CCCTGAGCTG 
GTGCAGTGGA AGAAGGAGCT GCGCGACGCC TACCGCGAGG CCCAGCAGCT GGTGCAGCGG 
GTGCCCAAGA TGAAGAACAA GCCGCGCTCG CCCGTGGTGG AGCTGAGCAA GGTGCCGCTG 
GTCCAGCGCG GCAGTGCCAA CGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK2 ( NM_001619.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001610.2 Homo sapiens G protein-coupled receptor kinase 2 (GRK2), mRNA.

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CloneID	OHu08965
Clone ID Related Accession (Same CDS sequence)	NM_001619.4 , NM_001619.3 , NM_001619.5
Accession Version	NM_001619.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-07
Organism	Homo sapiens(human)
Product	beta-adrenergic receptor kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC090863.1. On Nov 22, 2018 this sequence version replaced NM_001619.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC037963.1, SRR1163657.418571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000308595.10/ ENSP00000312262.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGACC TGGAGGCGGT GCTGGCCGAC GTGAGCTACC TGATGGCCAT GGAGAAGAGC 
AAGGCCACGC CGGCCGCGCG CGCCAGCAAG AAGATCCTGC TGCCCGAGCC CAGCATCCGC 
AGTGTCATGC AGAAGTACCT GGAGGACCGG GGCGAGGTGA CCTTTGAGAA GATCTTTTCC 
CAGAAGCTGG GGTACCTGCT CTTCCGAGAC TTCTGCCTGA ACCACCTGGA GGAGGCCAGG 
CCCTTGGTGG AATTCTATGA GGAGATCAAG AAGTACGAGA AGCTGGAGAC GGAGGAGGAG 
CGTGTGGCCC GCAGCCGGGA GATCTTCGAC TCATACATCA TGAAGGAGCT GCTGGCCTGC 
TCGCATCCCT TCTCGAAGAG TGCCACTGAG CATGTCCAAG GCCACCTGGG GAAGAAGCAG 
GTGCCTCCGG ATCTCTTCCA GCCATACATC GAAGAGATTT GTCAAAACCT CCGAGGGGAC 
GTGTTCCAGA AATTCATTGA GAGCGATAAG TTCACACGGT TTTGCCAGTG GAAGAATGTG 
GAGCTCAACA TCCACCTGAC CATGAATGAC TTCAGCGTGC ATCGCATCAT TGGGCGCGGG 
GGCTTTGGCG AGGTCTATGG GTGCCGGAAG GCTGACACAG GCAAGATGTA CGCCATGAAG 
TGCCTGGACA AAAAGCGCAT CAAGATGAAG CAGGGGGAGA CCCTGGCCCT GAACGAGCGC 
ATCATGCTCT CGCTCGTCAG CACTGGGGAC TGCCCATTCA TTGTCTGCAT GTCATACGCG 
TTCCACACGC CAGACAAGCT CAGCTTCATC CTGGACCTCA TGAACGGTGG GGACCTGCAC 
TACCACCTCT CCCAGCACGG GGTCTTCTCA GAGGCTGACA TGCGCTTCTA TGCGGCCGAG 
ATCATCCTGG GCCTGGAGCA CATGCACAAC CGCTTCGTGG TCTACCGGGA CCTGAAGCCA 
GCCAACATCC TTCTGGACGA GCATGGCCAC GTGCGGATCT CGGACCTGGG CCTGGCCTGT 
GACTTCTCCA AGAAGAAGCC CCATGCCAGC GTGGGCACCC ACGGGTACAT GGCTCCGGAG 
GTCCTGCAGA AGGGCGTGGC CTACGACAGC AGTGCCGACT GGTTCTCTCT GGGGTGCATG 
CTCTTCAAGT TGCTGCGGGG GCACAGCCCC TTCCGGCAGC ACAAGACCAA AGACAAGCAT 
GAGATCGACC GCATGACGCT GACGATGGCC GTGGAGCTGC CCGACTCCTT CTCCCCTGAA 
CTACGCTCCC TGCTGGAGGG GTTGCTGCAG AGGGATGTCA ACCGGAGATT GGGCTGCCTG 
GGCCGAGGGG CTCAGGAGGT GAAAGAGAGC CCCTTTTTCC GCTCCCTGGA CTGGCAGATG 
GTCTTCTTGC AGAAGTACCC TCCCCCGCTG ATCCCCCCAC GAGGGGAGGT GAACGCGGCC 
GACGCCTTCG ACATTGGCTC CTTCGATGAG GAGGACACAA AAGGAATCAA GTTACTGGAC 
AGTGATCAGG AGCTCTACCG CAACTTCCCC CTCACCATCT CGGAGCGGTG GCAGCAGGAG 
GTGGCAGAGA CTGTCTTCGA CACCATCAAC GCTGAGACAG ACCGGCTGGA GGCTCGCAAG 
AAAGCCAAGA ACAAGCAGCT GGGCCATGAG GAAGACTACG CCCTGGGCAA GGACTGCATC 
ATGCATGGCT ACATGTCCAA GATGGGCAAC CCCTTCCTGA CCCAGTGGCA GCGGCGGTAC 
TTCTACCTGT TCCCCAACCG CCTCGAGTGG CGGGGCGAGG GCGAGGCCCC GCAGAGCCTG 
CTGACCATGG AGGAGATCCA GTCGGTGGAG GAGACGCAGA TCAAGGAGCG CAAGTGCCTG 
CTCCTCAAGA TCCGCGGTGG GAAACAGTTC ATTTTGCAGT GCGATAGCGA CCCTGAGCTG 
GTGCAGTGGA AGAAGGAGCT GCGCGACGCC TACCGCGAGG CCCAGCAGCT GGTGCAGCGG 
GTGCCCAAGA TGAAGAACAA GCCGCGCTCG CCCGTGGTGG AGCTGAGCAA GGTGCCGCTG 
GTCCAGCGCG GCAGTGCCAA CGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001610.2
CDS	228..2297
Translation	MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLACSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL

Target ORF information:

RefSeq Version	NM_001619.5
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor kinase 2 (GRK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001619.5

ORF Insert Sequence:

ATGGCGGACC TGGAGGCGGT GCTGGCCGAC GTGAGCTACC TGATGGCCAT GGAGAAGAGC 
AAGGCCACGC CGGCCGCGCG CGCCAGCAAG AAGATCCTGC TGCCCGAGCC CAGCATCCGC 
AGTGTCATGC AGAAGTACCT GGAGGACCGG GGCGAGGTGA CCTTTGAGAA GATCTTTTCC 
CAGAAGCTGG GGTACCTGCT CTTCCGAGAC TTCTGCCTGA ACCACCTGGA GGAGGCCAGG 
CCCTTGGTGG AATTCTATGA GGAGATCAAG AAGTACGAGA AGCTGGAGAC GGAGGAGGAG 
CGTGTGGCCC GCAGCCGGGA GATCTTCGAC TCATACATCA TGAAGGAGCT GCTGGCCTGC 
TCGCATCCCT TCTCGAAGAG TGCCACTGAG CATGTCCAAG GCCACCTGGG GAAGAAGCAG 
GTGCCTCCGG ATCTCTTCCA GCCATACATC GAAGAGATTT GTCAAAACCT CCGAGGGGAC 
GTGTTCCAGA AATTCATTGA GAGCGATAAG TTCACACGGT TTTGCCAGTG GAAGAATGTG 
GAGCTCAACA TCCACCTGAC CATGAATGAC TTCAGCGTGC ATCGCATCAT TGGGCGCGGG 
GGCTTTGGCG AGGTCTATGG GTGCCGGAAG GCTGACACAG GCAAGATGTA CGCCATGAAG 
TGCCTGGACA AAAAGCGCAT CAAGATGAAG CAGGGGGAGA CCCTGGCCCT GAACGAGCGC 
ATCATGCTCT CGCTCGTCAG CACTGGGGAC TGCCCATTCA TTGTCTGCAT GTCATACGCG 
TTCCACACGC CAGACAAGCT CAGCTTCATC CTGGACCTCA TGAACGGTGG GGACCTGCAC 
TACCACCTCT CCCAGCACGG GGTCTTCTCA GAGGCTGACA TGCGCTTCTA TGCGGCCGAG 
ATCATCCTGG GCCTGGAGCA CATGCACAAC CGCTTCGTGG TCTACCGGGA CCTGAAGCCA 
GCCAACATCC TTCTGGACGA GCATGGCCAC GTGCGGATCT CGGACCTGGG CCTGGCCTGT 
GACTTCTCCA AGAAGAAGCC CCATGCCAGC GTGGGCACCC ACGGGTACAT GGCTCCGGAG 
GTCCTGCAGA AGGGCGTGGC CTACGACAGC AGTGCCGACT GGTTCTCTCT GGGGTGCATG 
CTCTTCAAGT TGCTGCGGGG GCACAGCCCC TTCCGGCAGC ACAAGACCAA AGACAAGCAT 
GAGATCGACC GCATGACGCT GACGATGGCC GTGGAGCTGC CCGACTCCTT CTCCCCTGAA 
CTACGCTCCC TGCTGGAGGG GTTGCTGCAG AGGGATGTCA ACCGGAGATT GGGCTGCCTG 
GGCCGAGGGG CTCAGGAGGT GAAAGAGAGC CCCTTTTTCC GCTCCCTGGA CTGGCAGATG 
GTCTTCTTGC AGAAGTACCC TCCCCCGCTG ATCCCCCCAC GAGGGGAGGT GAACGCGGCC 
GACGCCTTCG ACATTGGCTC CTTCGATGAG GAGGACACAA AAGGAATCAA GTTACTGGAC 
AGTGATCAGG AGCTCTACCG CAACTTCCCC CTCACCATCT CGGAGCGGTG GCAGCAGGAG 
GTGGCAGAGA CTGTCTTCGA CACCATCAAC GCTGAGACAG ACCGGCTGGA GGCTCGCAAG 
AAAGCCAAGA ACAAGCAGCT GGGCCATGAG GAAGACTACG CCCTGGGCAA GGACTGCATC 
ATGCATGGCT ACATGTCCAA GATGGGCAAC CCCTTCCTGA CCCAGTGGCA GCGGCGGTAC 
TTCTACCTGT TCCCCAACCG CCTCGAGTGG CGGGGCGAGG GCGAGGCCCC GCAGAGCCTG 
CTGACCATGG AGGAGATCCA GTCGGTGGAG GAGACGCAGA TCAAGGAGCG CAAGTGCCTG 
CTCCTCAAGA TCCGCGGTGG GAAACAGTTC ATTTTGCAGT GCGATAGCGA CCCTGAGCTG 
GTGCAGTGGA AGAAGGAGCT GCGCGACGCC TACCGCGAGG CCCAGCAGCT GGTGCAGCGG 
GTGCCCAAGA TGAAGAACAA GCCGCGCTCG CCCGTGGTGG AGCTGAGCAA GGTGCCGCTG 
GTCCAGCGCG GCAGTGCCAA CGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK2 ( XM_011544773.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543075.1 Homo sapiens G protein-coupled receptor kinase 2 (GRK2), transcript variant X2, mRNA.

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CloneID	OHu56194
Clone ID Related Accession (Same CDS sequence)	XM_011544773.1
Accession Version	XM_011544773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	beta-adrenergic receptor kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAAC TGAAGCTCCA GAGCATCCGC AGTGTCATGC AGAAGTACCT GGAGGACCGG 
GGCGAGGTGA CCTTTGAGAA GATCTTTTCC CAGAAGCTGG GGTACCTGCT CTTCCGAGAC 
TTCTGCCTGA ACCACCTGGA GGAGGCCAGG CCCTTGGTGG AATTCTATGA GGAGATCAAG 
AAGTACGAGA AGCTGGAGAC GGAGGAGGAG CGTGTGGCCC GCAGCCGGGA GATCTTCGAC 
TCATACATCA TGAAGGAGCT GCTGGCCTGC TCGCATCCCT TCTCGAAGAG TGCCACTGAG 
CATGTCCAAG GCCACCTGGG GAAGAAGCAG GTGCCTCCGG ATCTCTTCCA GCCATACATC 
GAAGAGATTT GTCAAAACCT CCGAGGGGAC GTGTTCCAGA AATTCATTGA GAGCGATAAG 
TTCACACGGT TTTGCCAGTG GAAGAATGTG GAGCTCAACA TCCACCTGAC CATGAATGAC 
TTCAGCGTGC ATCGCATCAT TGGGCGCGGG GGCTTTGGCG AGGTCTATGG GTGCCGGAAG 
GCTGACACAG GCAAGATGTA CGCCATGAAG TGCCTGGACA AAAAGCGCAT CAAGATGAAG 
CAGGGGGAGA CCCTGGCCCT GAACGAGCGC ATCATGCTCT CGCTCGTCAG CACTGGGGAC 
TGCCCATTCA TTGTCTGCAT GTCATACGCG TTCCACACGC CAGACAAGCT CAGCTTCATC 
CTGGACCTCA TGAACGGTGG GGACCTGCAC TACCACCTCT CCCAGCACGG GGTCTTCTCA 
GAGGCTGACA TGCGCTTCTA TGCGGCCGAG ATCATCCTGG GCCTGGAGCA CATGCACAAC 
CGCTTCGTGG TCTACCGGGA CCTGAAGCCA GCCAACATCC TTCTGGACGA GCATGGCCAC 
GTGCGGATCT CGGACCTGGG CCTGGCCTGT GACTTCTCCA AGAAGAAGCC CCATGCCAGC 
GTGGGCACCC ACGGGTACAT GGCTCCGGAG GTCCTGCAGA AGGGCGTGGC CTACGACAGC 
AGTGCCGACT GGTTCTCTCT GGGGTGCATG CTCTTCAAGT TGCTGCGGGG GCACAGCCCC 
TTCCGGCAGC ACAAGACCAA AGACAAGCAT GAGATCGACC GCATGACGCT GACGATGGCC 
GTGGAGCTGC CCGACTCCTT CTCCCCTGAA CTACGCTCCC TGCTGGAGGG GTTGCTGCAG 
AGGGATGTCA ACCGGAGATT GGGCTGCCTG GGCCGAGGGG CTCAGGAGGT GAAAGAGAGC 
CCCTTTTTCC GCTCCCTGGA CTGGCAGATG GTCTTCTTGC AGAAGTACCC TCCCCCGCTG 
ATCCCCCCAC GAGGGGAGGT GAACGCGGCC GACGCCTTCG ACATTGGCTC CTTCGATGAG 
GAGGACACAA AAGGAATCAA GTTACTGGAC AGTGATCAGG AGCTCTACCG CAACTTCCCC 
CTCACCATCT CGGAGCGGTG GCAGCAGGAG GTGGCAGAGA CTGTCTTCGA CACCATCAAC 
GCTGAGACAG ACCGGCTGGA GGCTCGCAAG AAAGCCAAGA ACAAGCAGCT GGGCCATGAG 
GAAGACTACG CCCTGGGCAA GGACTGCATC ATGCATGGCT ACATGTCCAA GATGGGCAAC 
CCCTTCCTGA CCCAGTGGCA GCGGCGGTAC TTCTACCTGT TCCCCAACCG CCTCGAGTGG 
CGGGGCGAGG GCGAGGCCCC GCAGAGCCTG CTGACCATGG AGGAGATCCA GTCGGTGGAG 
GAGACGCAGA TCAAGGAGCG CAAGTGCCTG CTCCTCAAGA TCCGCGGTGG GAAACAGTTC 
ATTTTGCAGT GCGATAGCGA CCCTGAGCTG GTGCAGTGGA AGAAGGAGCT GCGCGACGCC 
TACCGCGAGG CCCAGCAGCT GGTGCAGCGG GTGCCCAAGA TGAAGAACAA GCCGCGCTCG 
CCCGTGGTGG AGCTGAGCAA GGTGCCGCTG GTCCAGCGCG GCAGTGCCAA CGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543075.1
CDS	98..2077
Translation	MGKLKLQSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLACSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL

Target ORF information:

RefSeq Version	XM_011544773.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor kinase 2 (GRK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544773.1

ORF Insert Sequence:

ATGGGGAAAC TGAAGCTCCA GAGCATCCGC AGTGTCATGC AGAAGTACCT GGAGGACCGG 
GGCGAGGTGA CCTTTGAGAA GATCTTTTCC CAGAAGCTGG GGTACCTGCT CTTCCGAGAC 
TTCTGCCTGA ACCACCTGGA GGAGGCCAGG CCCTTGGTGG AATTCTATGA GGAGATCAAG 
AAGTACGAGA AGCTGGAGAC GGAGGAGGAG CGTGTGGCCC GCAGCCGGGA GATCTTCGAC 
TCATACATCA TGAAGGAGCT GCTGGCCTGC TCGCATCCCT TCTCGAAGAG TGCCACTGAG 
CATGTCCAAG GCCACCTGGG GAAGAAGCAG GTGCCTCCGG ATCTCTTCCA GCCATACATC 
GAAGAGATTT GTCAAAACCT CCGAGGGGAC GTGTTCCAGA AATTCATTGA GAGCGATAAG 
TTCACACGGT TTTGCCAGTG GAAGAATGTG GAGCTCAACA TCCACCTGAC CATGAATGAC 
TTCAGCGTGC ATCGCATCAT TGGGCGCGGG GGCTTTGGCG AGGTCTATGG GTGCCGGAAG 
GCTGACACAG GCAAGATGTA CGCCATGAAG TGCCTGGACA AAAAGCGCAT CAAGATGAAG 
CAGGGGGAGA CCCTGGCCCT GAACGAGCGC ATCATGCTCT CGCTCGTCAG CACTGGGGAC 
TGCCCATTCA TTGTCTGCAT GTCATACGCG TTCCACACGC CAGACAAGCT CAGCTTCATC 
CTGGACCTCA TGAACGGTGG GGACCTGCAC TACCACCTCT CCCAGCACGG GGTCTTCTCA 
GAGGCTGACA TGCGCTTCTA TGCGGCCGAG ATCATCCTGG GCCTGGAGCA CATGCACAAC 
CGCTTCGTGG TCTACCGGGA CCTGAAGCCA GCCAACATCC TTCTGGACGA GCATGGCCAC 
GTGCGGATCT CGGACCTGGG CCTGGCCTGT GACTTCTCCA AGAAGAAGCC CCATGCCAGC 
GTGGGCACCC ACGGGTACAT GGCTCCGGAG GTCCTGCAGA AGGGCGTGGC CTACGACAGC 
AGTGCCGACT GGTTCTCTCT GGGGTGCATG CTCTTCAAGT TGCTGCGGGG GCACAGCCCC 
TTCCGGCAGC ACAAGACCAA AGACAAGCAT GAGATCGACC GCATGACGCT GACGATGGCC 
GTGGAGCTGC CCGACTCCTT CTCCCCTGAA CTACGCTCCC TGCTGGAGGG GTTGCTGCAG 
AGGGATGTCA ACCGGAGATT GGGCTGCCTG GGCCGAGGGG CTCAGGAGGT GAAAGAGAGC 
CCCTTTTTCC GCTCCCTGGA CTGGCAGATG GTCTTCTTGC AGAAGTACCC TCCCCCGCTG 
ATCCCCCCAC GAGGGGAGGT GAACGCGGCC GACGCCTTCG ACATTGGCTC CTTCGATGAG 
GAGGACACAA AAGGAATCAA GTTACTGGAC AGTGATCAGG AGCTCTACCG CAACTTCCCC 
CTCACCATCT CGGAGCGGTG GCAGCAGGAG GTGGCAGAGA CTGTCTTCGA CACCATCAAC 
GCTGAGACAG ACCGGCTGGA GGCTCGCAAG AAAGCCAAGA ACAAGCAGCT GGGCCATGAG 
GAAGACTACG CCCTGGGCAA GGACTGCATC ATGCATGGCT ACATGTCCAA GATGGGCAAC 
CCCTTCCTGA CCCAGTGGCA GCGGCGGTAC TTCTACCTGT TCCCCAACCG CCTCGAGTGG 
CGGGGCGAGG GCGAGGCCCC GCAGAGCCTG CTGACCATGG AGGAGATCCA GTCGGTGGAG 
GAGACGCAGA TCAAGGAGCG CAAGTGCCTG CTCCTCAAGA TCCGCGGTGG GAAACAGTTC 
ATTTTGCAGT GCGATAGCGA CCCTGAGCTG GTGCAGTGGA AGAAGGAGCT GCGCGACGCC 
TACCGCGAGG CCCAGCAGCT GGTGCAGCGG GTGCCCAAGA TGAAGAACAA GCCGCGCTCG 
CCCGTGGTGG AGCTGAGCAA GGTGCCGCTG GTCCAGCGCG GCAGTGCCAA CGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK2 ( XM_024448376.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304144.1 Homo sapiens G protein-coupled receptor kinase 2 (GRK2), transcript variant X1, mRNA.

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CloneID	OHu106620
Clone ID Related Accession (Same CDS sequence)	XM_024448376.1
Accession Version	XM_024448376.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	beta-adrenergic receptor kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAGC CTGTCTTCCC CATACCCACA AGGTCAAGCA TCCGCAGTGT CATGCAGAAG 
TACCTGGAGG ACCGGGGCGA GGTGACCTTT GAGAAGATCT TTTCCCAGAA GCTGGGGTAC 
CTGCTCTTCC GAGACTTCTG CCTGAACCAC CTGGAGGAGG CCAGGCCCTT GGTGGAATTC 
TATGAGGAGA TCAAGAAGTA CGAGAAGCTG GAGACGGAGG AGGAGCGTGT GGCCCGCAGC 
CGGGAGATCT TCGACTCATA CATCATGAAG GAGCTGCTGG CCTGCTCGCA TCCCTTCTCG 
AAGAGTGCCA CTGAGCATGT CCAAGGCCAC CTGGGGAAGA AGCAGGTGCC TCCGGATCTC 
TTCCAGCCAT ACATCGAAGA GATTTGTCAA AACCTCCGAG GGGACGTGTT CCAGAAATTC 
ATTGAGAGCG ATAAGTTCAC ACGGTTTTGC CAGTGGAAGA ATGTGGAGCT CAACATCCAC 
CTGACCATGA ATGACTTCAG CGTGCATCGC ATCATTGGGC GCGGGGGCTT TGGCGAGGTC 
TATGGGTGCC GGAAGGCTGA CACAGGCAAG ATGTACGCCA TGAAGTGCCT GGACAAAAAG 
CGCATCAAGA TGAAGCAGGG GGAGACCCTG GCCCTGAACG AGCGCATCAT GCTCTCGCTC 
GTCAGCACTG GGGACTGCCC ATTCATTGTC TGCATGTCAT ACGCGTTCCA CACGCCAGAC 
AAGCTCAGCT TCATCCTGGA CCTCATGAAC GGTGGGGACC TGCACTACCA CCTCTCCCAG 
CACGGGGTCT TCTCAGAGGC TGACATGCGC TTCTATGCGG CCGAGATCAT CCTGGGCCTG 
GAGCACATGC ACAACCGCTT CGTGGTCTAC CGGGACCTGA AGCCAGCCAA CATCCTTCTG 
GACGAGCATG GCCACGTGCG GATCTCGGAC CTGGGCCTGG CCTGTGACTT CTCCAAGAAG 
AAGCCCCATG CCAGCGTGGG CACCCACGGG TACATGGCTC CGGAGGTCCT GCAGAAGGGC 
GTGGCCTACG ACAGCAGTGC CGACTGGTTC TCTCTGGGGT GCATGCTCTT CAAGTTGCTG 
CGGGGGCACA GCCCCTTCCG GCAGCACAAG ACCAAAGACA AGCATGAGAT CGACCGCATG 
ACGCTGACGA TGGCCGTGGA GCTGCCCGAC TCCTTCTCCC CTGAACTACG CTCCCTGCTG 
GAGGGGTTGC TGCAGAGGGA TGTCAACCGG AGATTGGGCT GCCTGGGCCG AGGGGCTCAG 
GAGGTGAAAG AGAGCCCCTT TTTCCGCTCC CTGGACTGGC AGATGGTCTT CTTGCAGAAG 
TACCCTCCCC CGCTGATCCC CCCACGAGGG GAGGTGAACG CGGCCGACGC CTTCGACATT 
GGCTCCTTCG ATGAGGAGGA CACAAAAGGA ATCAAGTTAC TGGACAGTGA TCAGGAGCTC 
TACCGCAACT TCCCCCTCAC CATCTCGGAG CGGTGGCAGC AGGAGGTGGC AGAGACTGTC 
TTCGACACCA TCAACGCTGA GACAGACCGG CTGGAGGCTC GCAAGAAAGC CAAGAACAAG 
CAGCTGGGCC ATGAGGAAGA CTACGCCCTG GGCAAGGACT GCATCATGCA TGGCTACATG 
TCCAAGATGG GCAACCCCTT CCTGACCCAG TGGCAGCGGC GGTACTTCTA CCTGTTCCCC 
AACCGCCTCG AGTGGCGGGG CGAGGGCGAG GCCCCGCAGA GCCTGCTGAC CATGGAGGAG 
ATCCAGTCGG TGGAGGAGAC GCAGATCAAG GAGCGCAAGT GCCTGCTCCT CAAGATCCGC 
GGTGGGAAAC AGTTCATTTT GCAGTGCGAT AGCGACCCTG AGCTGGTGCA GTGGAAGAAG 
GAGCTGCGCG ACGCCTACCG CGAGGCCCAG CAGCTGGTGC AGCGGGTGCC CAAGATGAAG 
AACAAGCCGC GCTCGCCCGT GGTGGAGCTG AGCAAGGTGC CGCTGGTCCA GCGCGGCAGT 
GCCAACGGCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304144.1
CDS	16..2010
Translation	MQEPVFPIPTRSSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLACSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL

Target ORF information:

RefSeq Version	XM_024448376.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor kinase 2 (GRK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448376.1

ORF Insert Sequence:

ATGCAGGAGC CTGTCTTCCC CATACCCACA AGGTCAAGCA TCCGCAGTGT CATGCAGAAG 
TACCTGGAGG ACCGGGGCGA GGTGACCTTT GAGAAGATCT TTTCCCAGAA GCTGGGGTAC 
CTGCTCTTCC GAGACTTCTG CCTGAACCAC CTGGAGGAGG CCAGGCCCTT GGTGGAATTC 
TATGAGGAGA TCAAGAAGTA CGAGAAGCTG GAGACGGAGG AGGAGCGTGT GGCCCGCAGC 
CGGGAGATCT TCGACTCATA CATCATGAAG GAGCTGCTGG CCTGCTCGCA TCCCTTCTCG 
AAGAGTGCCA CTGAGCATGT CCAAGGCCAC CTGGGGAAGA AGCAGGTGCC TCCGGATCTC 
TTCCAGCCAT ACATCGAAGA GATTTGTCAA AACCTCCGAG GGGACGTGTT CCAGAAATTC 
ATTGAGAGCG ATAAGTTCAC ACGGTTTTGC CAGTGGAAGA ATGTGGAGCT CAACATCCAC 
CTGACCATGA ATGACTTCAG CGTGCATCGC ATCATTGGGC GCGGGGGCTT TGGCGAGGTC 
TATGGGTGCC GGAAGGCTGA CACAGGCAAG ATGTACGCCA TGAAGTGCCT GGACAAAAAG 
CGCATCAAGA TGAAGCAGGG GGAGACCCTG GCCCTGAACG AGCGCATCAT GCTCTCGCTC 
GTCAGCACTG GGGACTGCCC ATTCATTGTC TGCATGTCAT ACGCGTTCCA CACGCCAGAC 
AAGCTCAGCT TCATCCTGGA CCTCATGAAC GGTGGGGACC TGCACTACCA CCTCTCCCAG 
CACGGGGTCT TCTCAGAGGC TGACATGCGC TTCTATGCGG CCGAGATCAT CCTGGGCCTG 
GAGCACATGC ACAACCGCTT CGTGGTCTAC CGGGACCTGA AGCCAGCCAA CATCCTTCTG 
GACGAGCATG GCCACGTGCG GATCTCGGAC CTGGGCCTGG CCTGTGACTT CTCCAAGAAG 
AAGCCCCATG CCAGCGTGGG CACCCACGGG TACATGGCTC CGGAGGTCCT GCAGAAGGGC 
GTGGCCTACG ACAGCAGTGC CGACTGGTTC TCTCTGGGGT GCATGCTCTT CAAGTTGCTG 
CGGGGGCACA GCCCCTTCCG GCAGCACAAG ACCAAAGACA AGCATGAGAT CGACCGCATG 
ACGCTGACGA TGGCCGTGGA GCTGCCCGAC TCCTTCTCCC CTGAACTACG CTCCCTGCTG 
GAGGGGTTGC TGCAGAGGGA TGTCAACCGG AGATTGGGCT GCCTGGGCCG AGGGGCTCAG 
GAGGTGAAAG AGAGCCCCTT TTTCCGCTCC CTGGACTGGC AGATGGTCTT CTTGCAGAAG 
TACCCTCCCC CGCTGATCCC CCCACGAGGG GAGGTGAACG CGGCCGACGC CTTCGACATT 
GGCTCCTTCG ATGAGGAGGA CACAAAAGGA ATCAAGTTAC TGGACAGTGA TCAGGAGCTC 
TACCGCAACT TCCCCCTCAC CATCTCGGAG CGGTGGCAGC AGGAGGTGGC AGAGACTGTC 
TTCGACACCA TCAACGCTGA GACAGACCGG CTGGAGGCTC GCAAGAAAGC CAAGAACAAG 
CAGCTGGGCC ATGAGGAAGA CTACGCCCTG GGCAAGGACT GCATCATGCA TGGCTACATG 
TCCAAGATGG GCAACCCCTT CCTGACCCAG TGGCAGCGGC GGTACTTCTA CCTGTTCCCC 
AACCGCCTCG AGTGGCGGGG CGAGGGCGAG GCCCCGCAGA GCCTGCTGAC CATGGAGGAG 
ATCCAGTCGG TGGAGGAGAC GCAGATCAAG GAGCGCAAGT GCCTGCTCCT CAAGATCCGC 
GGTGGGAAAC AGTTCATTTT GCAGTGCGAT AGCGACCCTG AGCTGGTGCA GTGGAAGAAG 
GAGCTGCGCG ACGCCTACCG CGAGGCCCAG CAGCTGGTGC AGCGGGTGCC CAAGATGAAG 
AACAAGCCGC GCTCGCCCGT GGTGGAGCTG AGCAAGGTGC CGCTGGTCCA GCGCGGCAGT 
GCCAACGGCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

?-Arrestin2 directly or through GRK2 inhibits PKC?II activation in a ubiquitination-dependent manner.
Biochimica et biophysica acta1865(1)142-157(2018 Jan)
Zhang X,Zheng M,Sun N,Kim KM

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GRK2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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