RASGEF1B cDNA ORF clone, Homo sapiens(human)

The following RASGEF1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGEF1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu06834 NM_152545.3
Latest version!
Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu38732 NM_001300735.2
Latest version!
Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OHu38734 NM_001300736.2
Latest version!
Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu06834 XM_017007813.1
Latest version!
Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu38733 XM_017007812.1
Latest version!
Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
View Old Accession Versions >>
OHu38734 NM_001300736.1 Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu06834 NM_152545.2 Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu38732 NM_001300735.1 Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu06834
    Clone ID Related Accession (Same CDS sequence) NM_152545.2 , XM_017007813.1 , NM_152545.3
    Accession Version NM_152545.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-01
    Organism Homo sapiens(human)
    Product ras-GEF domain-containing family member 1B isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK056257.1 and AC006287.1. On Jun 2, 2019 this sequence version replaced NM_152545.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK056257.1, SRR1803614.95265.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA
    ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG
    GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG
    AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA
    TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA
    ATAGCCAGTG GCGAAGAGCA GACATACAGA AAGAATGTCC AGCAAATGAT GCAGTGTCTG
    ATCCGCAAGC TTGCTGCGCT CAGCCAGTAC GAAGAAGTCC TGGCAAAAAT CAGCTCCACA
    TCCACAGATC GGCTCACAGT TCTCAAGACC AAGCCACAGT CTATACAAAG GGATATCATT
    ACTGTCTGCA ACGACCCTTA CACGTTGGCC CAGCAGCTGA CTCATATAGA GCTGGAGAGG
    CTCAATTATA TTGGGCCAGA AGAATTTGTT CAGGCGTTCG TGCAGAAGGA CCCTTTGGAT
    AATGACAAGA GTTGCTACAG TGAACGGAAG AAAACACGAA ACTTAGAAGC TTACGTGGAA
    TGGTTTAATC GCCTCAGCTA CTTGGTTGCT ACAGAAATCT GTATGCCTGT TAAGAAAAAA
    CACCGAGCAA GAATGATTGA GTATTTCATT GACGTAGCTC GGGAGTGTTT TAACATTGGC
    AACTTCAACT CCTTGATGGC GATAATCTCT GGTATGAATA TGAGCCCAGT CTCTCGACTA
    AAAAAAACTT GGGCCAAAGT GAAGACTGCA AAATTTGACA TTCTTGAGCA TCAGATGGAC
    CCTTCAAGCA ATTTCTATAA TTATCGAACA GCTCTTCGTG GGGCAGCACA AAGGTCTTTA
    ACTGCTCATA GTAGTAGAGA AAAGATTGTG ATACCATTCT TCAGTCTCTT AATCAAAGAT
    ATTTATTTCC TCAATGAGGG TTGTGCCAAC CGCCTTCCCA ATGGCCATGT CAATTTTGAG
    AAATTTTGGG AACTGGCCAA ACAAGTGAGT GAATTTATGA CATGGAAACA AGTGGAGTGT
    CCATTTGAGA GGGACCGGAA GATCTTGCAG TATCTGCTCA CAGTACCAGT CTTCAGTGAA
    GATGCTCTCT ACTTGGCTTC TTATGAGAGT GAAGGACCTG AAAATCATAT AGAGAAAGAC
    AGATGGAAGT CTTTAAGGTC GAGCCTCTTA GGCAGAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_689758.1
    CDS145..1566
    Translation

    Target ORF information:

    RefSeq Version NM_152545.3
    Organism Homo sapiens(human)
    Definition Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152545.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA
    ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG
    GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG
    AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA
    TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA
    ATAGCCAGTG GCGAAGAGCA GACATACAGA AAGAATGTCC AGCAAATGAT GCAGTGTCTG
    ATCCGCAAGC TTGCTGCGCT CAGCCAGTAC GAAGAAGTCC TGGCAAAAAT CAGCTCCACA
    TCCACAGATC GGCTCACAGT TCTCAAGACC AAGCCACAGT CTATACAAAG GGATATCATT
    ACTGTCTGCA ACGACCCTTA CACGTTGGCC CAGCAGCTGA CTCATATAGA GCTGGAGAGG
    CTCAATTATA TTGGGCCAGA AGAATTTGTT CAGGCGTTCG TGCAGAAGGA CCCTTTGGAT
    AATGACAAGA GTTGCTACAG TGAACGGAAG AAAACACGAA ACTTAGAAGC TTACGTGGAA
    TGGTTTAATC GCCTCAGCTA CTTGGTTGCT ACAGAAATCT GTATGCCTGT TAAGAAAAAA
    CACCGAGCAA GAATGATTGA GTATTTCATT GACGTAGCTC GGGAGTGTTT TAACATTGGC
    AACTTCAACT CCTTGATGGC GATAATCTCT GGTATGAATA TGAGCCCAGT CTCTCGACTA
    AAAAAAACTT GGGCCAAAGT GAAGACTGCA AAATTTGACA TTCTTGAGCA TCAGATGGAC
    CCTTCAAGCA ATTTCTATAA TTATCGAACA GCTCTTCGTG GGGCAGCACA AAGGTCTTTA
    ACTGCTCATA GTAGTAGAGA AAAGATTGTG ATACCATTCT TCAGTCTCTT AATCAAAGAT
    ATTTATTTCC TCAATGAGGG TTGTGCCAAC CGCCTTCCCA ATGGCCATGT CAATTTTGAG
    AAATTTTGGG AACTGGCCAA ACAAGTGAGT GAATTTATGA CATGGAAACA AGTGGAGTGT
    CCATTTGAGA GGGACCGGAA GATCTTGCAG TATCTGCTCA CAGTACCAGT CTTCAGTGAA
    GATGCTCTCT ACTTGGCTTC TTATGAGAGT GAAGGACCTG AAAATCATAT AGAGAAAGAC
    AGATGGAAGT CTTTAAGGTC GAGCCTCTTA GGCAGAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38732
    Clone ID Related Accession (Same CDS sequence) NM_001300735.1 , NM_001300735.2
    Accession Version NM_001300735.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1419bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-29
    Organism Homo sapiens(human)
    Product ras-GEF domain-containing family member 1B isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR002387.1, BC121003.1 and AC006287.1. On Jul 11, 2014 this sequence version replaced XM_005262776.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.206051.1, SRR1803613.42258.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA
    ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG
    GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG
    AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA
    TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA
    ATAGCCAGTG GCGAAGAGAC ATACAGAAAG AATGTCCAGC AAATGATGCA GTGTCTGATC
    CGCAAGCTTG CTGCGCTCAG CCAGTACGAA GAAGTCCTGG CAAAAATCAG CTCCACATCC
    ACAGATCGGC TCACAGTTCT CAAGACCAAG CCACAGTCTA TACAAAGGGA TATCATTACT
    GTCTGCAACG ACCCTTACAC GTTGGCCCAG CAGCTGACTC ATATAGAGCT GGAGAGGCTC
    AATTATATTG GGCCAGAAGA ATTTGTTCAG GCGTTCGTGC AGAAGGACCC TTTGGATAAT
    GACAAGAGTT GCTACAGTGA ACGGAAGAAA ACACGAAACT TAGAAGCTTA CGTGGAATGG
    TTTAATCGCC TCAGCTACTT GGTTGCTACA GAAATCTGTA TGCCTGTTAA GAAAAAACAC
    CGAGCAAGAA TGATTGAGTA TTTCATTGAC GTAGCTCGGG AGTGTTTTAA CATTGGCAAC
    TTCAACTCCT TGATGGCGAT AATCTCTGGT ATGAATATGA GCCCAGTCTC TCGACTAAAA
    AAAACTTGGG CCAAAGTGAA GACTGCAAAA TTTGACATTC TTGAGCATCA GATGGACCCT
    TCAAGCAATT TCTATAATTA TCGAACAGCT CTTCGTGGGG CAGCACAAAG GTCTTTAACT
    GCTCATAGTA GTAGAGAAAA GATTGTGATA CCATTCTTCA GTCTCTTAAT CAAAGATATT
    TATTTCCTCA ATGAGGGTTG TGCCAACCGC CTTCCCAATG GCCATGTCAA TTTTGAGAAA
    TTTTGGGAAC TGGCCAAACA AGTGAGTGAA TTTATGACAT GGAAACAAGT GGAGTGTCCA
    TTTGAGAGGG ACCGGAAGAT CTTGCAGTAT CTGCTCACAG TACCAGTCTT CAGTGAAGAT
    GCTCTCTACT TGGCTTCTTA TGAGAGTGAA GGACCTGAAA ATCATATAGA GAAAGACAGA
    TGGAAGTCTT TAAGGTCGAG CCTCTTAGGC AGAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287664.1
    CDS166..1584
    Translation

    Target ORF information:

    RefSeq Version NM_001300735.1
    Organism Homo sapiens(human)
    Definition Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300735.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA
    ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG
    GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG
    AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA
    TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA
    ATAGCCAGTG GCGAAGAGAC ATACAGAAAG AATGTCCAGC AAATGATGCA GTGTCTGATC
    CGCAAGCTTG CTGCGCTCAG CCAGTACGAA GAAGTCCTGG CAAAAATCAG CTCCACATCC
    ACAGATCGGC TCACAGTTCT CAAGACCAAG CCACAGTCTA TACAAAGGGA TATCATTACT
    GTCTGCAACG ACCCTTACAC GTTGGCCCAG CAGCTGACTC ATATAGAGCT GGAGAGGCTC
    AATTATATTG GGCCAGAAGA ATTTGTTCAG GCGTTCGTGC AGAAGGACCC TTTGGATAAT
    GACAAGAGTT GCTACAGTGA ACGGAAGAAA ACACGAAACT TAGAAGCTTA CGTGGAATGG
    TTTAATCGCC TCAGCTACTT GGTTGCTACA GAAATCTGTA TGCCTGTTAA GAAAAAACAC
    CGAGCAAGAA TGATTGAGTA TTTCATTGAC GTAGCTCGGG AGTGTTTTAA CATTGGCAAC
    TTCAACTCCT TGATGGCGAT AATCTCTGGT ATGAATATGA GCCCAGTCTC TCGACTAAAA
    AAAACTTGGG CCAAAGTGAA GACTGCAAAA TTTGACATTC TTGAGCATCA GATGGACCCT
    TCAAGCAATT TCTATAATTA TCGAACAGCT CTTCGTGGGG CAGCACAAAG GTCTTTAACT
    GCTCATAGTA GTAGAGAAAA GATTGTGATA CCATTCTTCA GTCTCTTAAT CAAAGATATT
    TATTTCCTCA ATGAGGGTTG TGCCAACCGC CTTCCCAATG GCCATGTCAA TTTTGAGAAA
    TTTTGGGAAC TGGCCAAACA AGTGAGTGAA TTTATGACAT GGAAACAAGT GGAGTGTCCA
    TTTGAGAGGG ACCGGAAGAT CTTGCAGTAT CTGCTCACAG TACCAGTCTT CAGTGAAGAT
    GCTCTCTACT TGGCTTCTTA TGAGAGTGAA GGACCTGAAA ATCATATAGA GAAAGACAGA
    TGGAAGTCTT TAAGGTCGAG CCTCTTAGGC AGAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38732
    Clone ID Related Accession (Same CDS sequence) NM_001300735.1 , NM_001300735.2
    Accession Version NM_001300735.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1419bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-09
    Organism Homo sapiens(human)
    Product ras-GEF domain-containing family member 1B isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR002387.1, BC121003.1 and AC006287.1. On Jun 2, 2019 this sequence version replaced NM_001300735.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.42258.1, SRR1660809.206051.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA
    ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG
    GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG
    AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA
    TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA
    ATAGCCAGTG GCGAAGAGAC ATACAGAAAG AATGTCCAGC AAATGATGCA GTGTCTGATC
    CGCAAGCTTG CTGCGCTCAG CCAGTACGAA GAAGTCCTGG CAAAAATCAG CTCCACATCC
    ACAGATCGGC TCACAGTTCT CAAGACCAAG CCACAGTCTA TACAAAGGGA TATCATTACT
    GTCTGCAACG ACCCTTACAC GTTGGCCCAG CAGCTGACTC ATATAGAGCT GGAGAGGCTC
    AATTATATTG GGCCAGAAGA ATTTGTTCAG GCGTTCGTGC AGAAGGACCC TTTGGATAAT
    GACAAGAGTT GCTACAGTGA ACGGAAGAAA ACACGAAACT TAGAAGCTTA CGTGGAATGG
    TTTAATCGCC TCAGCTACTT GGTTGCTACA GAAATCTGTA TGCCTGTTAA GAAAAAACAC
    CGAGCAAGAA TGATTGAGTA TTTCATTGAC GTAGCTCGGG AGTGTTTTAA CATTGGCAAC
    TTCAACTCCT TGATGGCGAT AATCTCTGGT ATGAATATGA GCCCAGTCTC TCGACTAAAA
    AAAACTTGGG CCAAAGTGAA GACTGCAAAA TTTGACATTC TTGAGCATCA GATGGACCCT
    TCAAGCAATT TCTATAATTA TCGAACAGCT CTTCGTGGGG CAGCACAAAG GTCTTTAACT
    GCTCATAGTA GTAGAGAAAA GATTGTGATA CCATTCTTCA GTCTCTTAAT CAAAGATATT
    TATTTCCTCA ATGAGGGTTG TGCCAACCGC CTTCCCAATG GCCATGTCAA TTTTGAGAAA
    TTTTGGGAAC TGGCCAAACA AGTGAGTGAA TTTATGACAT GGAAACAAGT GGAGTGTCCA
    TTTGAGAGGG ACCGGAAGAT CTTGCAGTAT CTGCTCACAG TACCAGTCTT CAGTGAAGAT
    GCTCTCTACT TGGCTTCTTA TGAGAGTGAA GGACCTGAAA ATCATATAGA GAAAGACAGA
    TGGAAGTCTT TAAGGTCGAG CCTCTTAGGC AGAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287664.1
    CDS145..1563
    Translation

    Target ORF information:

    RefSeq Version NM_001300735.2
    Organism Homo sapiens(human)
    Definition Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300735.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA
    ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG
    GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG
    AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA
    TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA
    ATAGCCAGTG GCGAAGAGAC ATACAGAAAG AATGTCCAGC AAATGATGCA GTGTCTGATC
    CGCAAGCTTG CTGCGCTCAG CCAGTACGAA GAAGTCCTGG CAAAAATCAG CTCCACATCC
    ACAGATCGGC TCACAGTTCT CAAGACCAAG CCACAGTCTA TACAAAGGGA TATCATTACT
    GTCTGCAACG ACCCTTACAC GTTGGCCCAG CAGCTGACTC ATATAGAGCT GGAGAGGCTC
    AATTATATTG GGCCAGAAGA ATTTGTTCAG GCGTTCGTGC AGAAGGACCC TTTGGATAAT
    GACAAGAGTT GCTACAGTGA ACGGAAGAAA ACACGAAACT TAGAAGCTTA CGTGGAATGG
    TTTAATCGCC TCAGCTACTT GGTTGCTACA GAAATCTGTA TGCCTGTTAA GAAAAAACAC
    CGAGCAAGAA TGATTGAGTA TTTCATTGAC GTAGCTCGGG AGTGTTTTAA CATTGGCAAC
    TTCAACTCCT TGATGGCGAT AATCTCTGGT ATGAATATGA GCCCAGTCTC TCGACTAAAA
    AAAACTTGGG CCAAAGTGAA GACTGCAAAA TTTGACATTC TTGAGCATCA GATGGACCCT
    TCAAGCAATT TCTATAATTA TCGAACAGCT CTTCGTGGGG CAGCACAAAG GTCTTTAACT
    GCTCATAGTA GTAGAGAAAA GATTGTGATA CCATTCTTCA GTCTCTTAAT CAAAGATATT
    TATTTCCTCA ATGAGGGTTG TGCCAACCGC CTTCCCAATG GCCATGTCAA TTTTGAGAAA
    TTTTGGGAAC TGGCCAAACA AGTGAGTGAA TTTATGACAT GGAAACAAGT GGAGTGTCCA
    TTTGAGAGGG ACCGGAAGAT CTTGCAGTAT CTGCTCACAG TACCAGTCTT CAGTGAAGAT
    GCTCTCTACT TGGCTTCTTA TGAGAGTGAA GGACCTGAAA ATCATATAGA GAAAGACAGA
    TGGAAGTCTT TAAGGTCGAG CCTCTTAGGC AGAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06834
    Clone ID Related Accession (Same CDS sequence) NM_152545.2 , XM_017007813.1 , NM_152545.3
    Accession Version NM_152545.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-29
    Organism Homo sapiens(human)
    Product ras-GEF domain-containing family member 1B isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR002387.1, AK056257.1 and AC006287.1. On Jul 11, 2014 this sequence version replaced NM_152545.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.158476.1, SRR1803614.95265.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA
    ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG
    GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG
    AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA
    TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA
    ATAGCCAGTG GCGAAGAGCA GACATACAGA AAGAATGTCC AGCAAATGAT GCAGTGTCTG
    ATCCGCAAGC TTGCTGCGCT CAGCCAGTAC GAAGAAGTCC TGGCAAAAAT CAGCTCCACA
    TCCACAGATC GGCTCACAGT TCTCAAGACC AAGCCACAGT CTATACAAAG GGATATCATT
    ACTGTCTGCA ACGACCCTTA CACGTTGGCC CAGCAGCTGA CTCATATAGA GCTGGAGAGG
    CTCAATTATA TTGGGCCAGA AGAATTTGTT CAGGCGTTCG TGCAGAAGGA CCCTTTGGAT
    AATGACAAGA GTTGCTACAG TGAACGGAAG AAAACACGAA ACTTAGAAGC TTACGTGGAA
    TGGTTTAATC GCCTCAGCTA CTTGGTTGCT ACAGAAATCT GTATGCCTGT TAAGAAAAAA
    CACCGAGCAA GAATGATTGA GTATTTCATT GACGTAGCTC GGGAGTGTTT TAACATTGGC
    AACTTCAACT CCTTGATGGC GATAATCTCT GGTATGAATA TGAGCCCAGT CTCTCGACTA
    AAAAAAACTT GGGCCAAAGT GAAGACTGCA AAATTTGACA TTCTTGAGCA TCAGATGGAC
    CCTTCAAGCA ATTTCTATAA TTATCGAACA GCTCTTCGTG GGGCAGCACA AAGGTCTTTA
    ACTGCTCATA GTAGTAGAGA AAAGATTGTG ATACCATTCT TCAGTCTCTT AATCAAAGAT
    ATTTATTTCC TCAATGAGGG TTGTGCCAAC CGCCTTCCCA ATGGCCATGT CAATTTTGAG
    AAATTTTGGG AACTGGCCAA ACAAGTGAGT GAATTTATGA CATGGAAACA AGTGGAGTGT
    CCATTTGAGA GGGACCGGAA GATCTTGCAG TATCTGCTCA CAGTACCAGT CTTCAGTGAA
    GATGCTCTCT ACTTGGCTTC TTATGAGAGT GAAGGACCTG AAAATCATAT AGAGAAAGAC
    AGATGGAAGT CTTTAAGGTC GAGCCTCTTA GGCAGAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_689758.1
    CDS166..1587
    Translation

    Target ORF information:

    RefSeq Version NM_152545.2
    Organism Homo sapiens(human)
    Definition Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152545.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA
    ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG
    GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG
    AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA
    TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA
    ATAGCCAGTG GCGAAGAGCA GACATACAGA AAGAATGTCC AGCAAATGAT GCAGTGTCTG
    ATCCGCAAGC TTGCTGCGCT CAGCCAGTAC GAAGAAGTCC TGGCAAAAAT CAGCTCCACA
    TCCACAGATC GGCTCACAGT TCTCAAGACC AAGCCACAGT CTATACAAAG GGATATCATT
    ACTGTCTGCA ACGACCCTTA CACGTTGGCC CAGCAGCTGA CTCATATAGA GCTGGAGAGG
    CTCAATTATA TTGGGCCAGA AGAATTTGTT CAGGCGTTCG TGCAGAAGGA CCCTTTGGAT
    AATGACAAGA GTTGCTACAG TGAACGGAAG AAAACACGAA ACTTAGAAGC TTACGTGGAA
    TGGTTTAATC GCCTCAGCTA CTTGGTTGCT ACAGAAATCT GTATGCCTGT TAAGAAAAAA
    CACCGAGCAA GAATGATTGA GTATTTCATT GACGTAGCTC GGGAGTGTTT TAACATTGGC
    AACTTCAACT CCTTGATGGC GATAATCTCT GGTATGAATA TGAGCCCAGT CTCTCGACTA
    AAAAAAACTT GGGCCAAAGT GAAGACTGCA AAATTTGACA TTCTTGAGCA TCAGATGGAC
    CCTTCAAGCA ATTTCTATAA TTATCGAACA GCTCTTCGTG GGGCAGCACA AAGGTCTTTA
    ACTGCTCATA GTAGTAGAGA AAAGATTGTG ATACCATTCT TCAGTCTCTT AATCAAAGAT
    ATTTATTTCC TCAATGAGGG TTGTGCCAAC CGCCTTCCCA ATGGCCATGT CAATTTTGAG
    AAATTTTGGG AACTGGCCAA ACAAGTGAGT GAATTTATGA CATGGAAACA AGTGGAGTGT
    CCATTTGAGA GGGACCGGAA GATCTTGCAG TATCTGCTCA CAGTACCAGT CTTCAGTGAA
    GATGCTCTCT ACTTGGCTTC TTATGAGAGT GAAGGACCTG AAAATCATAT AGAGAAAGAC
    AGATGGAAGT CTTTAAGGTC GAGCCTCTTA GGCAGAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38734
    Clone ID Related Accession (Same CDS sequence) NM_001300736.1 , NM_001300736.2
    Accession Version NM_001300736.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-29
    Organism Homo sapiens(human)
    Product ras-GEF domain-containing family member 1B isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR002387.1, BC121004.1 and AC006287.1. On Jul 11, 2014 this sequence version replaced XM_005262777.1. Transcript Variant: This variant (3) lacks an in-frame exon and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK303625.1, BC121004.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAAC
    CAGATGAGAA AAATTGCACC CAAAATCCTT CAACTCCTCA CGGAATGGAC GGAAACATTT
    CCCTATGATT TTCGGGATGA AAGAATGATG AGAAACTTAA AAGATCTGGC TCACCGAATA
    GCCAGTGGCG AAGAGACATA CAGAAAGAAT GTCCAGCAAA TGATGCAGTG TCTGATCCGC
    AAGCTTGCTG CGCTCAGCCA GTACGAAGAA GTCCTGGCAA AAATCAGCTC CACATCCACA
    GATCGGCTCA CAGTTCTCAA GACCAAGCCA CAGTCTATAC AAAGGGATAT CATTACTGTC
    TGCAACGACC CTTACACGTT GGCCCAGCAG CTGACTCATA TAGAGCTGGA GAGGCTCAAT
    TATATTGGGC CAGAAGAATT TGTTCAGGCG TTCGTGCAGA AGGACCCTTT GGATAATGAC
    AAGAGTTGCT ACAGTGAACG GAAGAAAACA CGAAACTTAG AAGCTTACGT GGAATGGTTT
    AATCGCCTCA GCTACTTGGT TGCTACAGAA ATCTGTATGC CTGTTAAGAA AAAACACCGA
    GCAAGAATGA TTGAGTATTT CATTGACGTA GCTCGGGAGT GTTTTAACAT TGGCAACTTC
    AACTCCTTGA TGGCGATAAT CTCTGGTATG AATATGAGCC CAGTCTCTCG ACTAAAAAAA
    ACTTGGGCCA AAGTGAAGAC TGCAAAATTT GACATTCTTG AGCATCAGAT GGACCCTTCA
    AGCAATTTCT ATAATTATCG AACAGCTCTT CGTGGGGCAG CACAAAGGTC TTTAACTGCT
    CATAGTAGTA GAGAAAAGAT TGTGATACCA TTCTTCAGTC TCTTAATCAA AGATATTTAT
    TTCCTCAATG AGGGTTGTGC CAACCGCCTT CCCAATGGCC ATGTCAATTT TGAGAAATTT
    TGGGAACTGG CCAAACAAGT GAGTGAATTT ATGACATGGA AACAAGTGGA GTGTCCATTT
    GAGAGGGACC GGAAGATCTT GCAGTATCTG CTCACAGTAC CAGTCTTCAG TGAAGATGCT
    CTCTACTTGG CTTCTTATGA GAGTGAAGGA CCTGAAAATC ATATAGAGAA AGACAGATGG
    AAGTCTTTAA GGTCGAGCCT CTTAGGCAGA GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287665.1
    CDS166..1461
    Translation

    Target ORF information:

    RefSeq Version NM_001300736.1
    Organism Homo sapiens(human)
    Definition Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAAC
    CAGATGAGAA AAATTGCACC CAAAATCCTT CAACTCCTCA CGGAATGGAC GGAAACATTT
    CCCTATGATT TTCGGGATGA AAGAATGATG AGAAACTTAA AAGATCTGGC TCACCGAATA
    GCCAGTGGCG AAGAGACATA CAGAAAGAAT GTCCAGCAAA TGATGCAGTG TCTGATCCGC
    AAGCTTGCTG CGCTCAGCCA GTACGAAGAA GTCCTGGCAA AAATCAGCTC CACATCCACA
    GATCGGCTCA CAGTTCTCAA GACCAAGCCA CAGTCTATAC AAAGGGATAT CATTACTGTC
    TGCAACGACC CTTACACGTT GGCCCAGCAG CTGACTCATA TAGAGCTGGA GAGGCTCAAT
    TATATTGGGC CAGAAGAATT TGTTCAGGCG TTCGTGCAGA AGGACCCTTT GGATAATGAC
    AAGAGTTGCT ACAGTGAACG GAAGAAAACA CGAAACTTAG AAGCTTACGT GGAATGGTTT
    AATCGCCTCA GCTACTTGGT TGCTACAGAA ATCTGTATGC CTGTTAAGAA AAAACACCGA
    GCAAGAATGA TTGAGTATTT CATTGACGTA GCTCGGGAGT GTTTTAACAT TGGCAACTTC
    AACTCCTTGA TGGCGATAAT CTCTGGTATG AATATGAGCC CAGTCTCTCG ACTAAAAAAA
    ACTTGGGCCA AAGTGAAGAC TGCAAAATTT GACATTCTTG AGCATCAGAT GGACCCTTCA
    AGCAATTTCT ATAATTATCG AACAGCTCTT CGTGGGGCAG CACAAAGGTC TTTAACTGCT
    CATAGTAGTA GAGAAAAGAT TGTGATACCA TTCTTCAGTC TCTTAATCAA AGATATTTAT
    TTCCTCAATG AGGGTTGTGC CAACCGCCTT CCCAATGGCC ATGTCAATTT TGAGAAATTT
    TGGGAACTGG CCAAACAAGT GAGTGAATTT ATGACATGGA AACAAGTGGA GTGTCCATTT
    GAGAGGGACC GGAAGATCTT GCAGTATCTG CTCACAGTAC CAGTCTTCAG TGAAGATGCT
    CTCTACTTGG CTTCTTATGA GAGTGAAGGA CCTGAAAATC ATATAGAGAA AGACAGATGG
    AAGTCTTTAA GGTCGAGCCT CTTAGGCAGA GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38734
    Clone ID Related Accession (Same CDS sequence) NM_001300736.1 , NM_001300736.2
    Accession Version NM_001300736.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-01
    Organism Homo sapiens(human)
    Product ras-GEF domain-containing family member 1B isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR002387.1, BC121004.1 and AC006287.1. On Jun 2, 2019 this sequence version replaced NM_001300736.1. Transcript Variant: This variant (3) lacks an in-frame exon and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC121004.1, AK303625.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAAC
    CAGATGAGAA AAATTGCACC CAAAATCCTT CAACTCCTCA CGGAATGGAC GGAAACATTT
    CCCTATGATT TTCGGGATGA AAGAATGATG AGAAACTTAA AAGATCTGGC TCACCGAATA
    GCCAGTGGCG AAGAGACATA CAGAAAGAAT GTCCAGCAAA TGATGCAGTG TCTGATCCGC
    AAGCTTGCTG CGCTCAGCCA GTACGAAGAA GTCCTGGCAA AAATCAGCTC CACATCCACA
    GATCGGCTCA CAGTTCTCAA GACCAAGCCA CAGTCTATAC AAAGGGATAT CATTACTGTC
    TGCAACGACC CTTACACGTT GGCCCAGCAG CTGACTCATA TAGAGCTGGA GAGGCTCAAT
    TATATTGGGC CAGAAGAATT TGTTCAGGCG TTCGTGCAGA AGGACCCTTT GGATAATGAC
    AAGAGTTGCT ACAGTGAACG GAAGAAAACA CGAAACTTAG AAGCTTACGT GGAATGGTTT
    AATCGCCTCA GCTACTTGGT TGCTACAGAA ATCTGTATGC CTGTTAAGAA AAAACACCGA
    GCAAGAATGA TTGAGTATTT CATTGACGTA GCTCGGGAGT GTTTTAACAT TGGCAACTTC
    AACTCCTTGA TGGCGATAAT CTCTGGTATG AATATGAGCC CAGTCTCTCG ACTAAAAAAA
    ACTTGGGCCA AAGTGAAGAC TGCAAAATTT GACATTCTTG AGCATCAGAT GGACCCTTCA
    AGCAATTTCT ATAATTATCG AACAGCTCTT CGTGGGGCAG CACAAAGGTC TTTAACTGCT
    CATAGTAGTA GAGAAAAGAT TGTGATACCA TTCTTCAGTC TCTTAATCAA AGATATTTAT
    TTCCTCAATG AGGGTTGTGC CAACCGCCTT CCCAATGGCC ATGTCAATTT TGAGAAATTT
    TGGGAACTGG CCAAACAAGT GAGTGAATTT ATGACATGGA AACAAGTGGA GTGTCCATTT
    GAGAGGGACC GGAAGATCTT GCAGTATCTG CTCACAGTAC CAGTCTTCAG TGAAGATGCT
    CTCTACTTGG CTTCTTATGA GAGTGAAGGA CCTGAAAATC ATATAGAGAA AGACAGATGG
    AAGTCTTTAA GGTCGAGCCT CTTAGGCAGA GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287665.1
    CDS145..1440
    Translation

    Target ORF information:

    RefSeq Version NM_001300736.2
    Organism Homo sapiens(human)
    Definition Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300736.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAAC
    CAGATGAGAA AAATTGCACC CAAAATCCTT CAACTCCTCA CGGAATGGAC GGAAACATTT
    CCCTATGATT TTCGGGATGA AAGAATGATG AGAAACTTAA AAGATCTGGC TCACCGAATA
    GCCAGTGGCG AAGAGACATA CAGAAAGAAT GTCCAGCAAA TGATGCAGTG TCTGATCCGC
    AAGCTTGCTG CGCTCAGCCA GTACGAAGAA GTCCTGGCAA AAATCAGCTC CACATCCACA
    GATCGGCTCA CAGTTCTCAA GACCAAGCCA CAGTCTATAC AAAGGGATAT CATTACTGTC
    TGCAACGACC CTTACACGTT GGCCCAGCAG CTGACTCATA TAGAGCTGGA GAGGCTCAAT
    TATATTGGGC CAGAAGAATT TGTTCAGGCG TTCGTGCAGA AGGACCCTTT GGATAATGAC
    AAGAGTTGCT ACAGTGAACG GAAGAAAACA CGAAACTTAG AAGCTTACGT GGAATGGTTT
    AATCGCCTCA GCTACTTGGT TGCTACAGAA ATCTGTATGC CTGTTAAGAA AAAACACCGA
    GCAAGAATGA TTGAGTATTT CATTGACGTA GCTCGGGAGT GTTTTAACAT TGGCAACTTC
    AACTCCTTGA TGGCGATAAT CTCTGGTATG AATATGAGCC CAGTCTCTCG ACTAAAAAAA
    ACTTGGGCCA AAGTGAAGAC TGCAAAATTT GACATTCTTG AGCATCAGAT GGACCCTTCA
    AGCAATTTCT ATAATTATCG AACAGCTCTT CGTGGGGCAG CACAAAGGTC TTTAACTGCT
    CATAGTAGTA GAGAAAAGAT TGTGATACCA TTCTTCAGTC TCTTAATCAA AGATATTTAT
    TTCCTCAATG AGGGTTGTGC CAACCGCCTT CCCAATGGCC ATGTCAATTT TGAGAAATTT
    TGGGAACTGG CCAAACAAGT GAGTGAATTT ATGACATGGA AACAAGTGGA GTGTCCATTT
    GAGAGGGACC GGAAGATCTT GCAGTATCTG CTCACAGTAC CAGTCTTCAG TGAAGATGCT
    CTCTACTTGG CTTCTTATGA GAGTGAAGGA CCTGAAAATC ATATAGAGAA AGACAGATGG
    AAGTCTTTAA GGTCGAGCCT CTTAGGCAGA GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06834
    Clone ID Related Accession (Same CDS sequence) NM_152545.2 , XM_017007813.1 , NM_152545.3
    Accession Version XM_017007813.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras-GEF domain-containing family member 1B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA
    ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG
    GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG
    AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA
    TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA
    ATAGCCAGTG GCGAAGAGCA GACATACAGA AAGAATGTCC AGCAAATGAT GCAGTGTCTG
    ATCCGCAAGC TTGCTGCGCT CAGCCAGTAC GAAGAAGTCC TGGCAAAAAT CAGCTCCACA
    TCCACAGATC GGCTCACAGT TCTCAAGACC AAGCCACAGT CTATACAAAG GGATATCATT
    ACTGTCTGCA ACGACCCTTA CACGTTGGCC CAGCAGCTGA CTCATATAGA GCTGGAGAGG
    CTCAATTATA TTGGGCCAGA AGAATTTGTT CAGGCGTTCG TGCAGAAGGA CCCTTTGGAT
    AATGACAAGA GTTGCTACAG TGAACGGAAG AAAACACGAA ACTTAGAAGC TTACGTGGAA
    TGGTTTAATC GCCTCAGCTA CTTGGTTGCT ACAGAAATCT GTATGCCTGT TAAGAAAAAA
    CACCGAGCAA GAATGATTGA GTATTTCATT GACGTAGCTC GGGAGTGTTT TAACATTGGC
    AACTTCAACT CCTTGATGGC GATAATCTCT GGTATGAATA TGAGCCCAGT CTCTCGACTA
    AAAAAAACTT GGGCCAAAGT GAAGACTGCA AAATTTGACA TTCTTGAGCA TCAGATGGAC
    CCTTCAAGCA ATTTCTATAA TTATCGAACA GCTCTTCGTG GGGCAGCACA AAGGTCTTTA
    ACTGCTCATA GTAGTAGAGA AAAGATTGTG ATACCATTCT TCAGTCTCTT AATCAAAGAT
    ATTTATTTCC TCAATGAGGG TTGTGCCAAC CGCCTTCCCA ATGGCCATGT CAATTTTGAG
    AAATTTTGGG AACTGGCCAA ACAAGTGAGT GAATTTATGA CATGGAAACA AGTGGAGTGT
    CCATTTGAGA GGGACCGGAA GATCTTGCAG TATCTGCTCA CAGTACCAGT CTTCAGTGAA
    GATGCTCTCT ACTTGGCTTC TTATGAGAGT GAAGGACCTG AAAATCATAT AGAGAAAGAC
    AGATGGAAGT CTTTAAGGTC GAGCCTCTTA GGCAGAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863302.1
    CDS254..1675
    Translation

    Target ORF information:

    RefSeq Version XM_017007813.1
    Organism Homo sapiens(human)
    Definition Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007813.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAGA
    ACATACATAT TTACCTTCCT ACTCAGTTCT CGGTTATTTA TGCATCCGTA TGAGCTAATG
    GCCAAAGTTT GCCACTTATG TGTTGAGCAC CAGAGACTAA GTGATCCTGA TAGTGATAAG
    AACCAGATGA GAAAAATTGC ACCCAAAATC CTTCAACTCC TCACGGAATG GACGGAAACA
    TTTCCCTATG ATTTTCGGGA TGAAAGAATG ATGAGAAACT TAAAAGATCT GGCTCACCGA
    ATAGCCAGTG GCGAAGAGCA GACATACAGA AAGAATGTCC AGCAAATGAT GCAGTGTCTG
    ATCCGCAAGC TTGCTGCGCT CAGCCAGTAC GAAGAAGTCC TGGCAAAAAT CAGCTCCACA
    TCCACAGATC GGCTCACAGT TCTCAAGACC AAGCCACAGT CTATACAAAG GGATATCATT
    ACTGTCTGCA ACGACCCTTA CACGTTGGCC CAGCAGCTGA CTCATATAGA GCTGGAGAGG
    CTCAATTATA TTGGGCCAGA AGAATTTGTT CAGGCGTTCG TGCAGAAGGA CCCTTTGGAT
    AATGACAAGA GTTGCTACAG TGAACGGAAG AAAACACGAA ACTTAGAAGC TTACGTGGAA
    TGGTTTAATC GCCTCAGCTA CTTGGTTGCT ACAGAAATCT GTATGCCTGT TAAGAAAAAA
    CACCGAGCAA GAATGATTGA GTATTTCATT GACGTAGCTC GGGAGTGTTT TAACATTGGC
    AACTTCAACT CCTTGATGGC GATAATCTCT GGTATGAATA TGAGCCCAGT CTCTCGACTA
    AAAAAAACTT GGGCCAAAGT GAAGACTGCA AAATTTGACA TTCTTGAGCA TCAGATGGAC
    CCTTCAAGCA ATTTCTATAA TTATCGAACA GCTCTTCGTG GGGCAGCACA AAGGTCTTTA
    ACTGCTCATA GTAGTAGAGA AAAGATTGTG ATACCATTCT TCAGTCTCTT AATCAAAGAT
    ATTTATTTCC TCAATGAGGG TTGTGCCAAC CGCCTTCCCA ATGGCCATGT CAATTTTGAG
    AAATTTTGGG AACTGGCCAA ACAAGTGAGT GAATTTATGA CATGGAAACA AGTGGAGTGT
    CCATTTGAGA GGGACCGGAA GATCTTGCAG TATCTGCTCA CAGTACCAGT CTTCAGTGAA
    GATGCTCTCT ACTTGGCTTC TTATGAGAGT GAAGGACCTG AAAATCATAT AGAGAAAGAC
    AGATGGAAGT CTTTAAGGTC GAGCCTCTTA GGCAGAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38733
    Clone ID Related Accession (Same CDS sequence) XM_017007812.1
    Accession Version XM_017007812.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras-GEF domain-containing family member 1B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAAC
    CAGATGAGAA AAATTGCACC CAAAATCCTT CAACTCCTCA CGGAATGGAC GGAAACATTT
    CCCTATGATT TTCGGGATGA AAGAATGATG AGAAACTTAA AAGATCTGGC TCACCGAATA
    GCCAGTGGCG AAGAGCAGAC ATACAGAAAG AATGTCCAGC AAATGATGCA GTGTCTGATC
    CGCAAGCTTG CTGCGCTCAG CCAGTACGAA GAAGTCCTGG CAAAAATCAG CTCCACATCC
    ACAGATCGGC TCACAGTTCT CAAGACCAAG CCACAGTCTA TACAAAGGGA TATCATTACT
    GTCTGCAACG ACCCTTACAC GTTGGCCCAG CAGCTGACTC ATATAGAGCT GGAGAGGCTC
    AATTATATTG GGCCAGAAGA ATTTGTTCAG GCGTTCGTGC AGAAGGACCC TTTGGATAAT
    GACAAGAGTT GCTACAGTGA ACGGAAGAAA ACACGAAACT TAGAAGCTTA CGTGGAATGG
    TTTAATCGCC TCAGCTACTT GGTTGCTACA GAAATCTGTA TGCCTGTTAA GAAAAAACAC
    CGAGCAAGAA TGATTGAGTA TTTCATTGAC GTAGCTCGGG AGTGTTTTAA CATTGGCAAC
    TTCAACTCCT TGATGGCGAT AATCTCTGGT ATGAATATGA GCCCAGTCTC TCGACTAAAA
    AAAACTTGGG CCAAAGTGAA GACTGCAAAA TTTGACATTC TTGAGCATCA GATGGACCCT
    TCAAGCAATT TCTATAATTA TCGAACAGCT CTTCGTGGGG CAGCACAAAG GTCTTTAACT
    GCTCATAGTA GTAGAGAAAA GATTGTGATA CCATTCTTCA GTCTCTTAAT CAAAGATATT
    TATTTCCTCA ATGAGGGTTG TGCCAACCGC CTTCCCAATG GCCATGTCAA TTTTGAGAAA
    TTTTGGGAAC TGGCCAAACA AGTGAGTGAA TTTATGACAT GGAAACAAGT GGAGTGTCCA
    TTTGAGAGGG ACCGGAAGAT CTTGCAGTAT CTGCTCACAG TACCAGTCTT CAGTGAAGAT
    GCTCTCTACT TGGCTTCTTA TGAGAGTGAA GGACCTGAAA ATCATATAGA GAAAGACAGA
    TGGAAGTCTT TAAGGTCGAG CCTCTTAGGC AGAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016863301.1
    CDS7..1305
    Translation

    Target ORF information:

    RefSeq Version XM_017007812.1
    Organism Homo sapiens(human)
    Definition Homo sapiens RasGEF domain family member 1B (RASGEF1B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007812.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCTCAGA CTCCTCCCTT TTCAGCAATG TTTGACAGCA GTGGTTACAA TCGAAACCTC 
    TATCAGTCTG CAGAGGACAG CTGTGGAGGG TTGTATTACC ATGACAACAA CCTCCTCTCT
    GGATCCCTGG AAGCACTCAT CCAGCACTTA GTACCTAATG TGGATTACTA TCCAGATAAC
    CAGATGAGAA AAATTGCACC CAAAATCCTT CAACTCCTCA CGGAATGGAC GGAAACATTT
    CCCTATGATT TTCGGGATGA AAGAATGATG AGAAACTTAA AAGATCTGGC TCACCGAATA
    GCCAGTGGCG AAGAGCAGAC ATACAGAAAG AATGTCCAGC AAATGATGCA GTGTCTGATC
    CGCAAGCTTG CTGCGCTCAG CCAGTACGAA GAAGTCCTGG CAAAAATCAG CTCCACATCC
    ACAGATCGGC TCACAGTTCT CAAGACCAAG CCACAGTCTA TACAAAGGGA TATCATTACT
    GTCTGCAACG ACCCTTACAC GTTGGCCCAG CAGCTGACTC ATATAGAGCT GGAGAGGCTC
    AATTATATTG GGCCAGAAGA ATTTGTTCAG GCGTTCGTGC AGAAGGACCC TTTGGATAAT
    GACAAGAGTT GCTACAGTGA ACGGAAGAAA ACACGAAACT TAGAAGCTTA CGTGGAATGG
    TTTAATCGCC TCAGCTACTT GGTTGCTACA GAAATCTGTA TGCCTGTTAA GAAAAAACAC
    CGAGCAAGAA TGATTGAGTA TTTCATTGAC GTAGCTCGGG AGTGTTTTAA CATTGGCAAC
    TTCAACTCCT TGATGGCGAT AATCTCTGGT ATGAATATGA GCCCAGTCTC TCGACTAAAA
    AAAACTTGGG CCAAAGTGAA GACTGCAAAA TTTGACATTC TTGAGCATCA GATGGACCCT
    TCAAGCAATT TCTATAATTA TCGAACAGCT CTTCGTGGGG CAGCACAAAG GTCTTTAACT
    GCTCATAGTA GTAGAGAAAA GATTGTGATA CCATTCTTCA GTCTCTTAAT CAAAGATATT
    TATTTCCTCA ATGAGGGTTG TGCCAACCGC CTTCCCAATG GCCATGTCAA TTTTGAGAAA
    TTTTGGGAAC TGGCCAAACA AGTGAGTGAA TTTATGACAT GGAAACAAGT GGAGTGTCCA
    TTTGAGAGGG ACCGGAAGAT CTTGCAGTAT CTGCTCACAG TACCAGTCTT CAGTGAAGAT
    GCTCTCTACT TGGCTTCTTA TGAGAGTGAA GGACCTGAAA ATCATATAGA GAAAGACAGA
    TGGAAGTCTT TAAGGTCGAG CCTCTTAGGC AGAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.