PUS10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PUS10
Entrez Gene ID	150962
Full Name	pseudouridylate synthase 10
Synonyms	CCDC139,DOBI
General protein information	Preferred Names pseudouridylate synthase 10 Names putative tRNA pseudouridine synthase Pus10 coiled-coil domain containing 139 coiled-coil domain-containing protein 139 pseudouridine synthase 10 psi55 synthase tRNA pseudouridine 55 synthase tRNA pseudouridylate synthase tRNA-uridine isomerase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p16.1-p15
Summary	Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009].
Disorder MIM:	612787

mRNA and Protein(s)

mRNA	Protein	Name
NM_144709.3	NP_653310.2	putative tRNA pseudouridine synthase Pus10 isoform 1
XM_006711952.2	XP_006712015.1	putative tRNA pseudouridine synthase Pus10 isoform X1
NM_144709.2	NP_653310.2	putative tRNA pseudouridine synthase Pus10
XM_011532574.2	XP_011530876.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_017003428.1	XP_016858917.1	putative tRNA pseudouridine synthase Pus10 isoform X3
XM_011532573.3	XP_011530875.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_017003429.2	XP_016858918.1	putative tRNA pseudouridine synthase Pus10 isoform X3
XM_011532578.1	XP_011530880.1	putative tRNA pseudouridine synthase Pus10 isoform X2
XM_017003429.1	XP_016858918.1	putative tRNA pseudouridine synthase Pus10 isoform X3
XM_011532576.2	XP_011530878.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532575.1	XP_011530877.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532568.1	XP_011530870.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532571.1	XP_011530873.1	putative tRNA pseudouridine synthase Pus10 isoform X1
NM_001322127.1	NP_001309056.1	putative tRNA pseudouridine synthase Pus10 isoform 2
XM_011532578.2	XP_011530880.1	putative tRNA pseudouridine synthase Pus10 isoform X2
XM_011532577.1	XP_011530879.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532576.1	XP_011530878.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532574.1	XP_011530876.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532573.1	XP_011530875.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532570.2	XP_011530872.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_024452720.1	XP_024308488.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532574.3	XP_011530876.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532576.3	XP_011530878.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_017003428.2	XP_016858917.1	putative tRNA pseudouridine synthase Pus10 isoform X3
XM_011532568.2	XP_011530870.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532572.1	XP_011530874.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532570.1	XP_011530872.1	putative tRNA pseudouridine synthase Pus10 isoform X1
NM_144709.4	NP_653310.2	putative tRNA pseudouridine synthase Pus10 isoform 1
XM_011532568.3	XP_011530870.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532571.2	XP_011530873.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532573.2	XP_011530875.1	putative tRNA pseudouridine synthase Pus10 isoform X1
XM_011532569.1	XP_011530871.1	putative tRNA pseudouridine synthase Pus10 isoform X1
NM_001322123.1	NP_001309052.1	putative tRNA pseudouridine synthase Pus10 isoform 1
NM_001322124.1	NP_001309053.1	putative tRNA pseudouridine synthase Pus10 isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PUS10	NP_001193766.1
	PUS10	XP_001115357.1
Pan troglodytes (chimpanzee)	PUS10	XP_515497.2
Canis lupus familiaris (dog)	PUS10	XP_005626182.1
Rattus norvegicus (Norway rat)	Pus10	NP_001020449.1
Caenorhabditis elegans (roundworm)	Y48C3A.20	NP_496819.1
Oryza sativa (rice)	Os04g0561700	NP_001053554.2
Homo sapiens (human)	PUS10	NP_653310.2
Mus musculus (house mouse)	Pus10	NP_001028826.1
Danio rerio (zebrafish)	pus10	NP_001104659.1
Drosophila melanogaster (fruit fly)	CG3709	NP_608500.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012003	XP_320532.3
Arabidopsis thaliana (thale cress)	AT1G20410	NP_173466.2
Gallus gallus (chicken)	PUS10	NP_001186378.1
Xenopus tropicalis (tropical clawed frog)	pus10	NP_001120259.1

Gene Ontology
Bibliography

Related articles in PubMed

Reciprocal amplification of caspase-3 activity by nuclear export of a putative human RNA-modifying protein, PUS10 during TRAIL-induced apoptosis.
Jana S, Hsieh AC, Gupta R
Cell death & disease8(10)e3093(2017 Oct)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease.
Jostins L, Ripke S, Weersma RK, Duerr RH, McGovern DP, Hui KY, Lee JC, Schumm LP, Sharma Y, Anderson CA, Essers J, Mitrovic M, Ning K, Cleynen I, Theatre E, Spain SL, Raychaudhuri S, Goyette P, Wei Z, Abraham C, Achkar JP, Ahmad T, Amininejad L, Ananthakrishnan AN, Andersen V, Andrews JM, Baidoo L, Balschun T, Bampton PA, Bitton A, Boucher G, Brand S, B?ning C, Cohain A, Cichon S, D'Amato M, De Jong D, Devaney KL, Dubinsky M, Edwards C, Ellinghaus D, Ferguson LR, Franchimont D, Fransen K, Gearry R, Georges M, Gieger C, Glas J, Haritunians T, Hart A, Hawkey C, Hedl M, Hu X, Karlsen TH, Kupcinskas L, Kugathasan S, Latiano A, Laukens D, Lawrance IC, Lees CW, Louis E, Mahy G, Mansfield J, Morgan AR, Mowat C, Newman W, Palmieri O, Ponsioen CY, Potocnik U, Prescott NJ, Regueiro M, Rotter JI, Russell RK, Sanderson JD, Sans M, Satsangi J, Schreiber S, Simms LA, Sventoraityte J, Targan SR, Taylor KD, Tremelling M, Verspaget HW, De Vos M, Wijmenga C, Wilson DC, Winkelmann J, Xavier RJ, Zeissig S, Zhang B, Zhang CK, Zhao H, , Silverberg MS, Annese V, Hakonarson H, Brant SR, Radford-Smith G, Mathew CG, Rioux JD, Schadt EE, Daly MJ, Franke A, Parkes M, Vermeire S, Barrett JC, Cho JH
Nature491(7422)119-24(2012 Nov)

A genome-wide scan of Ashkenazi Jewish Crohn's disease suggests novel susceptibility loci.
Kenny EE, Pe'er I, Karban A, Ozelius L, Mitchell AA, Ng SM, Erazo M, Ostrer H, Abraham C, Abreu MT, Atzmon G, Barzilai N, Brant SR, Bressman S, Burns ER, Chowers Y, Clark LN, Darvasi A, Doheny D, Duerr RH, Eliakim R, Giladi N, Gregersen PK, Hakonarson H, Jones MR, Marder K, McGovern DP, Mulle J, Orr-Urtreger A, Proctor DD, Pulver A, Rotter JI, Silverberg MS, Ullman T, Warren ST, Waterman M, Zhang W, Bergman A, Mayer L, Katz S, Desnick RJ, Cho JH, Peter I
PLoS genetics8(3)e1002559(2012)

A meta-analysis of genome-wide association scans identifies IL18RAP, PTPN2, TAGAP, and PUS10 as shared risk loci for Crohn's disease and celiac disease.
Festen EA, Goyette P, Green T, Boucher G, Beauchamp C, Trynka G, Dubois PC, Lagac? C, Stokkers PC, Hommes DW, Barisani D, Palmieri O, Annese V, van Heel DA, Weersma RK, Daly MJ, Wijmenga C, Rioux JD
PLoS genetics7(1)e1001283(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Evidence for significant overlap between common risk variants for Crohn's disease and ankylosing spondylitis.

Observational study of gene-disease association. (HuGE Navigator)
Title: Evidence for genes on chromosome 2 contributing to alcohol dependence with conduct disorder and suicide attempts.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity.

These data suggest that the cleaved DOBI may acquire a new function, possibly by cooperating with tBid in the mitochondrial event of cell death caused by TRAIL.
Title: DOBI is cleaved by caspases during TRAIL-induced apoptotic cell death.

Observational study of gene-disease association. (HuGE Navigator)
Title: Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling.

The following PUS10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUS10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24424	NM_144709.4 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	NM_001322123.1 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	NM_001322124.1 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532572.1 Latest version!	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532573.3 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532570.2 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532568.3 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532577.1 Latest version!	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532571.2 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532569.1 Latest version!	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532576.3 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_006711952.2 Latest version!	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532575.1 Latest version!	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532574.3 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_024452720.1 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu76592	XM_011532578.2 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu101551	NM_001322127.1 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu89058	XM_017003428.2 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu89058	XM_017003429.2 Latest version!	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu24424	NM_144709.2	Homo sapiens pseudouridylate synthase 10 (PUS10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu89058	XM_017003429.1	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu76592	XM_011532578.1	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu24424	XM_011532576.1	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532574.1	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532573.1	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532574.2	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532576.2	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu89058	XM_017003428.1	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu24424	XM_011532568.1	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532570.1	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	NM_144709.3	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532568.2	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532571.1	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24424	XM_011532573.2	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PUS10 ( NM_144709.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_653310.2 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant 1, mRNA.

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CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	NM_144709.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL832208.1, BP354604.1, BC101680.1, AC010733.8 and AA878516.1. On Apr 6, 2016 this sequence version replaced NM_144709.2. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.82250.1, AK056874.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653310.2
CDS	268..1857
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	NM_144709.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144709.3

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( NM_144709.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_653310.2 Homo sapiens pseudouridylate synthase 10 (PUS10), mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	NM_144709.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	putative tRNA pseudouridine synthase Pus10
Comment

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_653310.2

Translation

MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	NM_144709.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144709.2

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( NM_144709.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_653310.2 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	NM_144709.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL832208.1, BP354604.1, BC101680.1, AC010733.8 and AA878516.1. On Nov 23, 2018 this sequence version replaced NM_144709.3. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.82250.1, AK056874.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000316752.11/ ENSP00000326003.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653310.2
CDS	264..1853
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	NM_144709.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144709.4

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( NM_001322123.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309052.1 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	NM_001322123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC010733.8, BC101680.1 and AA878516.1. On Apr 6, 2016 this sequence version replaced XM_006711952.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.154081.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309052.1
CDS	211..1800
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	NM_001322123.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322123.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( NM_001322124.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309053.1 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant 3, mRNA.

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CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	NM_001322124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AW503429.1, AK291729.1, BC143480.1, BP226056.1, AC010733.8 and AA878516.1. On Apr 6, 2016 this sequence version replaced XM_011532569.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.203580.1, AK291729.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309053.1
CDS	80..1669
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	NM_001322124.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322124.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_006711952.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712015.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X1, mRNA.

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CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_006711952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006712015.1

Translation

MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_006711952.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006711952.2

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532574.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530876.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532574.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532574.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530876.1
CDS	161..1750
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532574.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532574.2

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532573.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530875.1 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532573.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011532573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530875.1
CDS	100..1689
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532573.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532573.3

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532576.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530878.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532576.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532576.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530878.1
CDS	220..1809
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532576.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532576.2

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532575.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530877.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011530877.1

Translation

MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532575.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532575.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532568.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530870.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532568.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011530870.1

Translation

MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532568.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532568.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532571.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530873.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532571.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530873.1
CDS	220..1809
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532571.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532571.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532577.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530879.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011530879.1

Translation

MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532577.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532577.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532576.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530878.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532576.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011530878.1

Translation

MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532576.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532576.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532574.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530876.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532574.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011530876.1

Translation

MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532574.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532574.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532573.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530875.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532573.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011530875.1

Translation

MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532573.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532573.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532570.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530872.1 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532570.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011532570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530872.1
CDS	46..1635
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532570.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532570.2

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_024452720.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308488.1 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_024452720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308488.1
CDS	309..1898
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_024452720.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452720.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532574.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530876.1 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532574.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011532574.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530876.1
CDS	1571..3160
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532574.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532574.3

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532576.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530878.1 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X7, mRNA.

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CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532576.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011532576.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530878.1
CDS	220..1809
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532576.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532576.3

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532568.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530870.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532568.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532568.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530870.1
CDS	339..1928
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532568.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532568.2

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532572.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530874.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530874.1
CDS	140..1729
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532572.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532572.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532570.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530872.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532570.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530872.1
CDS	46..1635
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532570.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532570.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532568.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530870.1 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532568.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011532568.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530870.1
CDS	232..1821
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532568.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532568.3

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532571.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530873.1 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532571.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011532571.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530873.1
CDS	220..1809
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532571.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532571.2

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532573.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530875.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532573.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530875.1
CDS	100..1689
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532573.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532573.2

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532569.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530871.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu24424
Clone ID Related Accession (Same CDS sequence)	NM_144709.3 , XM_006711952.2 , NM_144709.2 , XM_011532574.2 , XM_011532573.3 , XM_011532576.2 , XM_011532575.1 , XM_011532568.1 , XM_011532571.1 , XM_011532577.1 , XM_011532576.1 , XM_011532574.1 , XM_011532573.1 , XM_011532570.2 , XM_024452720.1 , XM_011532574.3 , XM_011532576.3 , XM_011532568.2 , XM_011532572.1 , XM_011532570.1 , NM_144709.4 , XM_011532568.3 , XM_011532571.2 , XM_011532573.2 , XM_011532569.1 , NM_001322123.1 , NM_001322124.1
Accession Version	XM_011532569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X1
Comment

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011530871.1

Translation

MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELILEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532569.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532569.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGAGT TGCTCAATGA ACTACAGAAA TTTCTGGAAA CTGAAAAAGA TGAATTAATT 
TTGGAAGTTA TGAACCCACC TCCCAAGAAA ATTCGACTGC AAGAACTGGA AGATAGTATT 
GATAATCTAA GTCAAAATGG AGAGGGAAGG ATCTCTGTTA GTCATGTTGG AAGCACTGCT 
TCCAAGAACT CAAATTTAAA TGTATGTAAT GTATGCCTAG GAATTCTTCA AGAATTCTGT 
GAGAAAGATT TCATTAAAAA GGTGTGCCAA AAGGTTGAGG CCTCTGGGTT TGAATTCACC 
AGCTTGGTAT TTTCAGTCTC CTTCCCACCA CAACTATCTG TAAGAGAGCA TGCTGCATGG 
TTGCTGGTAA AACAGGAAAT GGGAAAGCAG AGTCTGTCGC TGGGAAGAGA TGATATAGTT 
CAGCTAAAAG AAGCCTACAA ATGGATAACT CACCCCCTGT TTTCAGAGGA ACTGGGTGTT 
CCCATTGATG GAAAGAGCTT GTTTGAAGTG AGTGTGGTCT TTGCTCACCC AGAAACAGTT 
GAGGATTGCC ACTTCCTAGC TGCGATATGC CCAGATTGTT TTAAGCCAGC CAAAAACAAA 
CAGTCTGTAT TCACTAGAAT GGCAGTTATG AAAGCCTTGA ATAAGATAAA GGAAGAGGAT 
TTCCTTAAGC AGTTTCCTTG TCCTCCAAAC TCACCAAAGG CTGTATGCGC TGTTCTTGAA 
ATTGAATGTG CTCATGGTGC TGTTTTTGTG GCTGGGAGAT ATAATAAATA CTCCAGGAAT 
CTACCACAAA CTCCTTGGAT AATTGATGGA GAAAGGAAGC TGGAATCTTC AGTGGAAGAA 
TTAATTTCAG ATCATCTGTT GGCAGTATTT AAAGCAGAGA GTTTTAATTT TTCATCCTCT 
GGAAGAGAAG ATGTAGATGT GAGAACATTA GGAAATGGAA GGCCCTTTGC AATTGAGCTG 
GTGAATCCTC ATAGAGTACA TTTCACTTCA CAAGAAATTA AGGAACTTCA GCAGAAAATT 
AATAACTCAT CTAACAAAAT CCAAGTACGT GACTTGCAGC TTGTCACAAG AGAGGCAATA 
GGACATATGA AAGAAGGTGA AGAAGAAAAG ACAAAGACCT ACAGTGCCTT AATTTGGACA 
AATAAAGCGA TACAGAAGAA AGACATTGAA TTCCTAAATG ACATAAAGGA CTTAAAAATC 
GACCAGAAAA CACCTTTGCG CGTCCTTCAC CGAAGGCCCC TGGCTGTGCG AGCTCGCGTC 
ATTCACTTCA TGGAGACACA GTACGTGGAT GAGCACCACT TCCGCCTCCA CTTGAAAACT 
CAGGCTGGCA CCTACATTAA AGAGTTTGTA CATGGAGACT TCGGGAGAAC CAAGCCAAAC 
ATTGGGTCCC TGATGAATGT GACTGCAGAC ATTCTGGAGC TGGATGTTGA GTCTGTAGAT 
GTTGACTGGC CACCTGCTCT GGATGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532578.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530880.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X8, mRNA.

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CloneID	OHu76592
Clone ID Related Accession (Same CDS sequence)	XM_011532578.1 , XM_011532578.2
Accession Version	XM_011532578.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCAC CTCCCAAGAA AATTCGACTG CAAGAACTGG AAGATAGTAT TGATAATCTA 
AGTCAAAATG GAGAGGGAAG GATCTCTGTT AGTCATGTTG GAAGCACTGC TTCCAAGAAC 
TCAAATTTAA ATGTATGTAA TGTATGCCTA GGAATTCTTC AAGAATTCTG TGAGAAAGAT 
TTCATTAAAA AGGTGTGCCA AAAGGTTGAG GCCTCTGGGT TTGAATTCAC CAGCTTGGTA 
TTTTCAGTCT CCTTCCCACC ACAACTATCT GTAAGAGAGC ATGCTGCATG GTTGCTGGTA 
AAACAGGAAA TGGGAAAGCA GAGTCTGTCG CTGGGAAGAG ATGATATAGT TCAGCTAAAA 
GAAGCCTACA AATGGATAAC TCACCCCCTG TTTTCAGAGG AACTGGGTGT TCCCATTGAT 
GGAAAGAGCT TGTTTGAAGT GAGTGTGGTC TTTGCTCACC CAGAAACAGT TGAGGATTGC 
CACTTCCTAG CTGCGATATG CCCAGATTGT TTTAAGCCAG CCAAAAACAA ACAGTCTGTA 
TTCACTAGAA TGGCAGTTAT GAAAGCCTTG AATAAGATAA AGGAAGAGGA TTTCCTTAAG 
CAGTTTCCTT GTCCTCCAAA CTCACCAAAG GCTGTATGCG CTGTTCTTGA AATTGAATGT 
GCTCATGGTG CTGTTTTTGT GGCTGGGAGA TATAATAAAT ACTCCAGGAA TCTACCACAA 
ACTCCTTGGA TAATTGATGG AGAAAGGAAG CTGGAATCTT CAGTGGAAGA ATTAATTTCA 
GATCATCTGT TGGCAGTATT TAAAGCAGAG AGTTTTAATT TTTCATCCTC TGGAAGAGAA 
GATGTAGATG TGAGAACATT AGGAAATGGA AGGCCCTTTG CAATTGAGCT GGTGAATCCT 
CATAGAGTAC ATTTCACTTC ACAAGAAATT AAGGAACTTC AGCAGAAAAT TAATAACTCA 
TCTAACAAAA TCCAAGTACG TGACTTGCAG CTTGTCACAA GAGAGGCAAT AGGACATATG 
AAAGAAGGTG AAGAAGAAAA GACAAAGACC TACAGTGCCT TAATTTGGAC AAATAAAGCG 
ATACAGAAGA AAGACATTGA ATTCCTAAAT GACATAAAGG ACTTAAAAAT CGACCAGAAA 
ACACCTTTGC GCGTCCTTCA CCGAAGGCCC CTGGCTGTGC GAGCTCGCGT CATTCACTTC 
ATGGAGACAC AGTACGTGGA TGAGCACCAC TTCCGCCTCC ACTTGAAAAC TCAGGCTGGC 
ACCTACATTA AAGAGTTTGT ACATGGAGAC TTCGGGAGAA CCAAGCCAAA CATTGGGTCC 
CTGATGAATG TGACTGCAGA CATTCTGGAG CTGGATGTTG AGTCTGTAGA TGTTGACTGG 
CCACCTGCTC TGGATGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530880.1
CDS	387..1787
Translation	MNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532578.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532578.1

ORF Insert Sequence:

ATGAACCCAC CTCCCAAGAA AATTCGACTG CAAGAACTGG AAGATAGTAT TGATAATCTA 
AGTCAAAATG GAGAGGGAAG GATCTCTGTT AGTCATGTTG GAAGCACTGC TTCCAAGAAC 
TCAAATTTAA ATGTATGTAA TGTATGCCTA GGAATTCTTC AAGAATTCTG TGAGAAAGAT 
TTCATTAAAA AGGTGTGCCA AAAGGTTGAG GCCTCTGGGT TTGAATTCAC CAGCTTGGTA 
TTTTCAGTCT CCTTCCCACC ACAACTATCT GTAAGAGAGC ATGCTGCATG GTTGCTGGTA 
AAACAGGAAA TGGGAAAGCA GAGTCTGTCG CTGGGAAGAG ATGATATAGT TCAGCTAAAA 
GAAGCCTACA AATGGATAAC TCACCCCCTG TTTTCAGAGG AACTGGGTGT TCCCATTGAT 
GGAAAGAGCT TGTTTGAAGT GAGTGTGGTC TTTGCTCACC CAGAAACAGT TGAGGATTGC 
CACTTCCTAG CTGCGATATG CCCAGATTGT TTTAAGCCAG CCAAAAACAA ACAGTCTGTA 
TTCACTAGAA TGGCAGTTAT GAAAGCCTTG AATAAGATAA AGGAAGAGGA TTTCCTTAAG 
CAGTTTCCTT GTCCTCCAAA CTCACCAAAG GCTGTATGCG CTGTTCTTGA AATTGAATGT 
GCTCATGGTG CTGTTTTTGT GGCTGGGAGA TATAATAAAT ACTCCAGGAA TCTACCACAA 
ACTCCTTGGA TAATTGATGG AGAAAGGAAG CTGGAATCTT CAGTGGAAGA ATTAATTTCA 
GATCATCTGT TGGCAGTATT TAAAGCAGAG AGTTTTAATT TTTCATCCTC TGGAAGAGAA 
GATGTAGATG TGAGAACATT AGGAAATGGA AGGCCCTTTG CAATTGAGCT GGTGAATCCT 
CATAGAGTAC ATTTCACTTC ACAAGAAATT AAGGAACTTC AGCAGAAAAT TAATAACTCA 
TCTAACAAAA TCCAAGTACG TGACTTGCAG CTTGTCACAA GAGAGGCAAT AGGACATATG 
AAAGAAGGTG AAGAAGAAAA GACAAAGACC TACAGTGCCT TAATTTGGAC AAATAAAGCG 
ATACAGAAGA AAGACATTGA ATTCCTAAAT GACATAAAGG ACTTAAAAAT CGACCAGAAA 
ACACCTTTGC GCGTCCTTCA CCGAAGGCCC CTGGCTGTGC GAGCTCGCGT CATTCACTTC 
ATGGAGACAC AGTACGTGGA TGAGCACCAC TTCCGCCTCC ACTTGAAAAC TCAGGCTGGC 
ACCTACATTA AAGAGTTTGT ACATGGAGAC TTCGGGAGAA CCAAGCCAAA CATTGGGTCC 
CTGATGAATG TGACTGCAGA CATTCTGGAG CTGGATGTTG AGTCTGTAGA TGTTGACTGG 
CCACCTGCTC TGGATGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_011532578.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530880.1 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X8, mRNA.

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CloneID	OHu76592
Clone ID Related Accession (Same CDS sequence)	XM_011532578.1 , XM_011532578.2
Accession Version	XM_011532578.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011532578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCAC CTCCCAAGAA AATTCGACTG CAAGAACTGG AAGATAGTAT TGATAATCTA 
AGTCAAAATG GAGAGGGAAG GATCTCTGTT AGTCATGTTG GAAGCACTGC TTCCAAGAAC 
TCAAATTTAA ATGTATGTAA TGTATGCCTA GGAATTCTTC AAGAATTCTG TGAGAAAGAT 
TTCATTAAAA AGGTGTGCCA AAAGGTTGAG GCCTCTGGGT TTGAATTCAC CAGCTTGGTA 
TTTTCAGTCT CCTTCCCACC ACAACTATCT GTAAGAGAGC ATGCTGCATG GTTGCTGGTA 
AAACAGGAAA TGGGAAAGCA GAGTCTGTCG CTGGGAAGAG ATGATATAGT TCAGCTAAAA 
GAAGCCTACA AATGGATAAC TCACCCCCTG TTTTCAGAGG AACTGGGTGT TCCCATTGAT 
GGAAAGAGCT TGTTTGAAGT GAGTGTGGTC TTTGCTCACC CAGAAACAGT TGAGGATTGC 
CACTTCCTAG CTGCGATATG CCCAGATTGT TTTAAGCCAG CCAAAAACAA ACAGTCTGTA 
TTCACTAGAA TGGCAGTTAT GAAAGCCTTG AATAAGATAA AGGAAGAGGA TTTCCTTAAG 
CAGTTTCCTT GTCCTCCAAA CTCACCAAAG GCTGTATGCG CTGTTCTTGA AATTGAATGT 
GCTCATGGTG CTGTTTTTGT GGCTGGGAGA TATAATAAAT ACTCCAGGAA TCTACCACAA 
ACTCCTTGGA TAATTGATGG AGAAAGGAAG CTGGAATCTT CAGTGGAAGA ATTAATTTCA 
GATCATCTGT TGGCAGTATT TAAAGCAGAG AGTTTTAATT TTTCATCCTC TGGAAGAGAA 
GATGTAGATG TGAGAACATT AGGAAATGGA AGGCCCTTTG CAATTGAGCT GGTGAATCCT 
CATAGAGTAC ATTTCACTTC ACAAGAAATT AAGGAACTTC AGCAGAAAAT TAATAACTCA 
TCTAACAAAA TCCAAGTACG TGACTTGCAG CTTGTCACAA GAGAGGCAAT AGGACATATG 
AAAGAAGGTG AAGAAGAAAA GACAAAGACC TACAGTGCCT TAATTTGGAC AAATAAAGCG 
ATACAGAAGA AAGACATTGA ATTCCTAAAT GACATAAAGG ACTTAAAAAT CGACCAGAAA 
ACACCTTTGC GCGTCCTTCA CCGAAGGCCC CTGGCTGTGC GAGCTCGCGT CATTCACTTC 
ATGGAGACAC AGTACGTGGA TGAGCACCAC TTCCGCCTCC ACTTGAAAAC TCAGGCTGGC 
ACCTACATTA AAGAGTTTGT ACATGGAGAC TTCGGGAGAA CCAAGCCAAA CATTGGGTCC 
CTGATGAATG TGACTGCAGA CATTCTGGAG CTGGATGTTG AGTCTGTAGA TGTTGACTGG 
CCACCTGCTC TGGATGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530880.1
CDS	387..1787
Translation	MNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFCEKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_011532578.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532578.2

ORF Insert Sequence:

ATGAACCCAC CTCCCAAGAA AATTCGACTG CAAGAACTGG AAGATAGTAT TGATAATCTA 
AGTCAAAATG GAGAGGGAAG GATCTCTGTT AGTCATGTTG GAAGCACTGC TTCCAAGAAC 
TCAAATTTAA ATGTATGTAA TGTATGCCTA GGAATTCTTC AAGAATTCTG TGAGAAAGAT 
TTCATTAAAA AGGTGTGCCA AAAGGTTGAG GCCTCTGGGT TTGAATTCAC CAGCTTGGTA 
TTTTCAGTCT CCTTCCCACC ACAACTATCT GTAAGAGAGC ATGCTGCATG GTTGCTGGTA 
AAACAGGAAA TGGGAAAGCA GAGTCTGTCG CTGGGAAGAG ATGATATAGT TCAGCTAAAA 
GAAGCCTACA AATGGATAAC TCACCCCCTG TTTTCAGAGG AACTGGGTGT TCCCATTGAT 
GGAAAGAGCT TGTTTGAAGT GAGTGTGGTC TTTGCTCACC CAGAAACAGT TGAGGATTGC 
CACTTCCTAG CTGCGATATG CCCAGATTGT TTTAAGCCAG CCAAAAACAA ACAGTCTGTA 
TTCACTAGAA TGGCAGTTAT GAAAGCCTTG AATAAGATAA AGGAAGAGGA TTTCCTTAAG 
CAGTTTCCTT GTCCTCCAAA CTCACCAAAG GCTGTATGCG CTGTTCTTGA AATTGAATGT 
GCTCATGGTG CTGTTTTTGT GGCTGGGAGA TATAATAAAT ACTCCAGGAA TCTACCACAA 
ACTCCTTGGA TAATTGATGG AGAAAGGAAG CTGGAATCTT CAGTGGAAGA ATTAATTTCA 
GATCATCTGT TGGCAGTATT TAAAGCAGAG AGTTTTAATT TTTCATCCTC TGGAAGAGAA 
GATGTAGATG TGAGAACATT AGGAAATGGA AGGCCCTTTG CAATTGAGCT GGTGAATCCT 
CATAGAGTAC ATTTCACTTC ACAAGAAATT AAGGAACTTC AGCAGAAAAT TAATAACTCA 
TCTAACAAAA TCCAAGTACG TGACTTGCAG CTTGTCACAA GAGAGGCAAT AGGACATATG 
AAAGAAGGTG AAGAAGAAAA GACAAAGACC TACAGTGCCT TAATTTGGAC AAATAAAGCG 
ATACAGAAGA AAGACATTGA ATTCCTAAAT GACATAAAGG ACTTAAAAAT CGACCAGAAA 
ACACCTTTGC GCGTCCTTCA CCGAAGGCCC CTGGCTGTGC GAGCTCGCGT CATTCACTTC 
ATGGAGACAC AGTACGTGGA TGAGCACCAC TTCCGCCTCC ACTTGAAAAC TCAGGCTGGC 
ACCTACATTA AAGAGTTTGT ACATGGAGAC TTCGGGAGAA CCAAGCCAAA CATTGGGTCC 
CTGATGAATG TGACTGCAGA CATTCTGGAG CTGGATGTTG AGTCTGTAGA TGTTGACTGG 
CCACCTGCTC TGGATGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( NM_001322127.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001309056.1 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant 4, mRNA.

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CloneID	OHu101551
Clone ID Related Accession (Same CDS sequence)	NM_001322127.1
Accession Version	NM_001322127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL832208.1, BP354604.1, BC143480.1, AC010733.8 and AA878516.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143480.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAGAG CTGCGATATG CCCAGATTGT TTTAAGCCAG CCAAAAACAA ACAGTCTGTA 
TTCACTAGAA TGGCAGTTAT GAAAGCCTTG AATAAGATAA AGGAAGAGGA TTTCCTTAAG 
CAGTTTCCTT GTCCTCCAAA CTCACCAAAG GCTGTATGCG CTGTTCTTGA AATTGAATGT 
GCTCATGGTG CTGTTTTTGT GGCTGGGAGA TATAATAAAT ACTCCAGGAA TCTACCACAA 
ACTCCTTGGA TAATTGATGG AGAAAGGAAG CTGGAATCTT CAGTGGAAGA ATTAATTTCA 
GATCATCTGT TGGCAGTATT TAAAGCAGAG AGTTTTAATT TTTCATCCTC TGGAAGAGAA 
GATGTAGATG TGAGAACATT AGGAAATGGA AGGCCCTTTG CAATTGAGCT GGTGAATCCT 
CATAGAGTAC ATTTCACTTC ACAAGAAATT AAGGAACTTC AGCAGAAAAT TAATAACTCA 
TCTAACAAAA TCCAAGTACG TGACTTGCAG CTTGTCACAA GAGAGGCAAT AGGACATATG 
AAAGAAGGTG AAGAAGAAAA GACAAAGACC TACAGTGCCT TAATTTGGAC AAATAAAGCG 
ATACAGAAGA AAGACATTGA ATTCCTAAAT GACATAAAGG ACTTAAAAAT CGACCAGAAA 
ACACCTTTGC GCGTCCTTCA CCGAAGGCCC CTGGCTGTGC GAGCTCGCGT CATTCACTTC 
ATGGAGACAC AGTACGTGGA TGAGCACCAC TTCCGCCTCC ACTTGAAAAC TCAGGCTGGC 
ACCTACATTA AAGAGTTTGT ACATGGAGAC TTCGGGAGAA CCAAGCCAAA CATTGGGTCC 
CTGATGAATG TGACTGCAGA CATTCTGGAG CTGGATGTTG AGTCTGTAGA TGTTGACTGG 
CCACCTGCTC TGGATGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001309056.1
CDS	875..1795
Translation	MERAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	NM_001322127.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001322127.1

ORF Insert Sequence:

ATGGAAAGAG CTGCGATATG CCCAGATTGT TTTAAGCCAG CCAAAAACAA ACAGTCTGTA 
TTCACTAGAA TGGCAGTTAT GAAAGCCTTG AATAAGATAA AGGAAGAGGA TTTCCTTAAG 
CAGTTTCCTT GTCCTCCAAA CTCACCAAAG GCTGTATGCG CTGTTCTTGA AATTGAATGT 
GCTCATGGTG CTGTTTTTGT GGCTGGGAGA TATAATAAAT ACTCCAGGAA TCTACCACAA 
ACTCCTTGGA TAATTGATGG AGAAAGGAAG CTGGAATCTT CAGTGGAAGA ATTAATTTCA 
GATCATCTGT TGGCAGTATT TAAAGCAGAG AGTTTTAATT TTTCATCCTC TGGAAGAGAA 
GATGTAGATG TGAGAACATT AGGAAATGGA AGGCCCTTTG CAATTGAGCT GGTGAATCCT 
CATAGAGTAC ATTTCACTTC ACAAGAAATT AAGGAACTTC AGCAGAAAAT TAATAACTCA 
TCTAACAAAA TCCAAGTACG TGACTTGCAG CTTGTCACAA GAGAGGCAAT AGGACATATG 
AAAGAAGGTG AAGAAGAAAA GACAAAGACC TACAGTGCCT TAATTTGGAC AAATAAAGCG 
ATACAGAAGA AAGACATTGA ATTCCTAAAT GACATAAAGG ACTTAAAAAT CGACCAGAAA 
ACACCTTTGC GCGTCCTTCA CCGAAGGCCC CTGGCTGTGC GAGCTCGCGT CATTCACTTC 
ATGGAGACAC AGTACGTGGA TGAGCACCAC TTCCGCCTCC ACTTGAAAAC TCAGGCTGGC 
ACCTACATTA AAGAGTTTGT ACATGGAGAC TTCGGGAGAA CCAAGCCAAA CATTGGGTCC 
CTGATGAATG TGACTGCAGA CATTCTGGAG CTGGATGTTG AGTCTGTAGA TGTTGACTGG 
CCACCTGCTC TGGATGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_017003428.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858917.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X9, mRNA.

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CloneID	OHu89058
Clone ID Related Accession (Same CDS sequence)	XM_017003428.1 , XM_017003429.2 , XM_017003429.1 , XM_017003428.2
Accession Version	XM_017003428.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGTGT GCCAAAAGGT TGAGGCCTCT GGGTTTGAAT TCACCAGCTT GGTATTTTCA 
GTCTCCTTCC CACCACAACT ATCTGTAAGA GAGCATGCTG CATGGTTGCT GGTAAAACAG 
GAAATGGGAA AGCAGAGTCT GTCGCTGGGA AGAGATGATA TAGTTCAGCT AAAAGAAGCC 
TACAAATGGA TAACTCACCC CCTGTTTTCA GAGGAACTGG GTGTTCCCAT TGATGGAAAG 
AGCTTGTTTG AAGTGAGTGT GGTCTTTGCT CACCCAGAAA CAGTTGAGGA TTGCCACTTC 
CTAGCTGCGA TATGCCCAGA TTGTTTTAAG CCAGCCAAAA ACAAACAGTC TGTATTCACT 
AGAATGGCAG TTATGAAAGC CTTGAATAAG ATAAAGGAAG AGGATTTCCT TAAGCAGTTT 
CCTTGTCCTC CAAACTCACC AAAGGCTGTA TGCGCTGTTC TTGAAATTGA ATGTGCTCAT 
GGTGCTGTTT TTGTGGCTGG GAGATATAAT AAATACTCCA GGAATCTACC ACAAACTCCT 
TGGATAATTG ATGGAGAAAG GAAGCTGGAA TCTTCAGTGG AAGAATTAAT TTCAGATCAT 
CTGTTGGCAG TATTTAAAGC AGAGAGTTTT AATTTTTCAT CCTCTGGAAG AGAAGATGTA 
GATGTGAGAA CATTAGGAAA TGGAAGGCCC TTTGCAATTG AGCTGGTGAA TCCTCATAGA 
GTACATTTCA CTTCACAAGA AATTAAGGAA CTTCAGCAGA AAATTAATAA CTCATCTAAC 
AAAATCCAAG TACGTGACTT GCAGCTTGTC ACAAGAGAGG CAATAGGACA TATGAAAGAA 
GGTGAAGAAG AAAAGACAAA GACCTACAGT GCCTTAATTT GGACAAATAA AGCGATACAG 
AAGAAAGACA TTGAATTCCT AAATGACATA AAGGACTTAA AAATCGACCA GAAAACACCT 
TTGCGCGTCC TTCACCGAAG GCCCCTGGCT GTGCGAGCTC GCGTCATTCA CTTCATGGAG 
ACACAGTACG TGGATGAGCA CCACTTCCGC CTCCACTTGA AAACTCAGGC TGGCACCTAC 
ATTAAAGAGT TTGTACATGG AGACTTCGGG AGAACCAAGC CAAACATTGG GTCCCTGATG 
AATGTGACTG CAGACATTCT GGAGCTGGAT GTTGAGTCTG TAGATGTTGA CTGGCCACCT 
GCTCTGGATG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858917.1
CDS	292..1626
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_017003428.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003428.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGTGT GCCAAAAGGT TGAGGCCTCT GGGTTTGAAT TCACCAGCTT GGTATTTTCA 
GTCTCCTTCC CACCACAACT ATCTGTAAGA GAGCATGCTG CATGGTTGCT GGTAAAACAG 
GAAATGGGAA AGCAGAGTCT GTCGCTGGGA AGAGATGATA TAGTTCAGCT AAAAGAAGCC 
TACAAATGGA TAACTCACCC CCTGTTTTCA GAGGAACTGG GTGTTCCCAT TGATGGAAAG 
AGCTTGTTTG AAGTGAGTGT GGTCTTTGCT CACCCAGAAA CAGTTGAGGA TTGCCACTTC 
CTAGCTGCGA TATGCCCAGA TTGTTTTAAG CCAGCCAAAA ACAAACAGTC TGTATTCACT 
AGAATGGCAG TTATGAAAGC CTTGAATAAG ATAAAGGAAG AGGATTTCCT TAAGCAGTTT 
CCTTGTCCTC CAAACTCACC AAAGGCTGTA TGCGCTGTTC TTGAAATTGA ATGTGCTCAT 
GGTGCTGTTT TTGTGGCTGG GAGATATAAT AAATACTCCA GGAATCTACC ACAAACTCCT 
TGGATAATTG ATGGAGAAAG GAAGCTGGAA TCTTCAGTGG AAGAATTAAT TTCAGATCAT 
CTGTTGGCAG TATTTAAAGC AGAGAGTTTT AATTTTTCAT CCTCTGGAAG AGAAGATGTA 
GATGTGAGAA CATTAGGAAA TGGAAGGCCC TTTGCAATTG AGCTGGTGAA TCCTCATAGA 
GTACATTTCA CTTCACAAGA AATTAAGGAA CTTCAGCAGA AAATTAATAA CTCATCTAAC 
AAAATCCAAG TACGTGACTT GCAGCTTGTC ACAAGAGAGG CAATAGGACA TATGAAAGAA 
GGTGAAGAAG AAAAGACAAA GACCTACAGT GCCTTAATTT GGACAAATAA AGCGATACAG 
AAGAAAGACA TTGAATTCCT AAATGACATA AAGGACTTAA AAATCGACCA GAAAACACCT 
TTGCGCGTCC TTCACCGAAG GCCCCTGGCT GTGCGAGCTC GCGTCATTCA CTTCATGGAG 
ACACAGTACG TGGATGAGCA CCACTTCCGC CTCCACTTGA AAACTCAGGC TGGCACCTAC 
ATTAAAGAGT TTGTACATGG AGACTTCGGG AGAACCAAGC CAAACATTGG GTCCCTGATG 
AATGTGACTG CAGACATTCT GGAGCTGGAT GTTGAGTCTG TAGATGTTGA CTGGCCACCT 
GCTCTGGATG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_017003429.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858918.1 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu89058
Clone ID Related Accession (Same CDS sequence)	XM_017003428.1 , XM_017003429.2 , XM_017003429.1 , XM_017003428.2
Accession Version	XM_017003429.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGTGT GCCAAAAGGT TGAGGCCTCT GGGTTTGAAT TCACCAGCTT GGTATTTTCA 
GTCTCCTTCC CACCACAACT ATCTGTAAGA GAGCATGCTG CATGGTTGCT GGTAAAACAG 
GAAATGGGAA AGCAGAGTCT GTCGCTGGGA AGAGATGATA TAGTTCAGCT AAAAGAAGCC 
TACAAATGGA TAACTCACCC CCTGTTTTCA GAGGAACTGG GTGTTCCCAT TGATGGAAAG 
AGCTTGTTTG AAGTGAGTGT GGTCTTTGCT CACCCAGAAA CAGTTGAGGA TTGCCACTTC 
CTAGCTGCGA TATGCCCAGA TTGTTTTAAG CCAGCCAAAA ACAAACAGTC TGTATTCACT 
AGAATGGCAG TTATGAAAGC CTTGAATAAG ATAAAGGAAG AGGATTTCCT TAAGCAGTTT 
CCTTGTCCTC CAAACTCACC AAAGGCTGTA TGCGCTGTTC TTGAAATTGA ATGTGCTCAT 
GGTGCTGTTT TTGTGGCTGG GAGATATAAT AAATACTCCA GGAATCTACC ACAAACTCCT 
TGGATAATTG ATGGAGAAAG GAAGCTGGAA TCTTCAGTGG AAGAATTAAT TTCAGATCAT 
CTGTTGGCAG TATTTAAAGC AGAGAGTTTT AATTTTTCAT CCTCTGGAAG AGAAGATGTA 
GATGTGAGAA CATTAGGAAA TGGAAGGCCC TTTGCAATTG AGCTGGTGAA TCCTCATAGA 
GTACATTTCA CTTCACAAGA AATTAAGGAA CTTCAGCAGA AAATTAATAA CTCATCTAAC 
AAAATCCAAG TACGTGACTT GCAGCTTGTC ACAAGAGAGG CAATAGGACA TATGAAAGAA 
GGTGAAGAAG AAAAGACAAA GACCTACAGT GCCTTAATTT GGACAAATAA AGCGATACAG 
AAGAAAGACA TTGAATTCCT AAATGACATA AAGGACTTAA AAATCGACCA GAAAACACCT 
TTGCGCGTCC TTCACCGAAG GCCCCTGGCT GTGCGAGCTC GCGTCATTCA CTTCATGGAG 
ACACAGTACG TGGATGAGCA CCACTTCCGC CTCCACTTGA AAACTCAGGC TGGCACCTAC 
ATTAAAGAGT TTGTACATGG AGACTTCGGG AGAACCAAGC CAAACATTGG GTCCCTGATG 
AATGTGACTG CAGACATTCT GGAGCTGGAT GTTGAGTCTG TAGATGTTGA CTGGCCACCT 
GCTCTGGATG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858918.1
CDS	88..1422
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_017003429.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003429.2

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGTGT GCCAAAAGGT TGAGGCCTCT GGGTTTGAAT TCACCAGCTT GGTATTTTCA 
GTCTCCTTCC CACCACAACT ATCTGTAAGA GAGCATGCTG CATGGTTGCT GGTAAAACAG 
GAAATGGGAA AGCAGAGTCT GTCGCTGGGA AGAGATGATA TAGTTCAGCT AAAAGAAGCC 
TACAAATGGA TAACTCACCC CCTGTTTTCA GAGGAACTGG GTGTTCCCAT TGATGGAAAG 
AGCTTGTTTG AAGTGAGTGT GGTCTTTGCT CACCCAGAAA CAGTTGAGGA TTGCCACTTC 
CTAGCTGCGA TATGCCCAGA TTGTTTTAAG CCAGCCAAAA ACAAACAGTC TGTATTCACT 
AGAATGGCAG TTATGAAAGC CTTGAATAAG ATAAAGGAAG AGGATTTCCT TAAGCAGTTT 
CCTTGTCCTC CAAACTCACC AAAGGCTGTA TGCGCTGTTC TTGAAATTGA ATGTGCTCAT 
GGTGCTGTTT TTGTGGCTGG GAGATATAAT AAATACTCCA GGAATCTACC ACAAACTCCT 
TGGATAATTG ATGGAGAAAG GAAGCTGGAA TCTTCAGTGG AAGAATTAAT TTCAGATCAT 
CTGTTGGCAG TATTTAAAGC AGAGAGTTTT AATTTTTCAT CCTCTGGAAG AGAAGATGTA 
GATGTGAGAA CATTAGGAAA TGGAAGGCCC TTTGCAATTG AGCTGGTGAA TCCTCATAGA 
GTACATTTCA CTTCACAAGA AATTAAGGAA CTTCAGCAGA AAATTAATAA CTCATCTAAC 
AAAATCCAAG TACGTGACTT GCAGCTTGTC ACAAGAGAGG CAATAGGACA TATGAAAGAA 
GGTGAAGAAG AAAAGACAAA GACCTACAGT GCCTTAATTT GGACAAATAA AGCGATACAG 
AAGAAAGACA TTGAATTCCT AAATGACATA AAGGACTTAA AAATCGACCA GAAAACACCT 
TTGCGCGTCC TTCACCGAAG GCCCCTGGCT GTGCGAGCTC GCGTCATTCA CTTCATGGAG 
ACACAGTACG TGGATGAGCA CCACTTCCGC CTCCACTTGA AAACTCAGGC TGGCACCTAC 
ATTAAAGAGT TTGTACATGG AGACTTCGGG AGAACCAAGC CAAACATTGG GTCCCTGATG 
AATGTGACTG CAGACATTCT GGAGCTGGAT GTTGAGTCTG TAGATGTTGA CTGGCCACCT 
GCTCTGGATG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_017003429.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858918.1 Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu89058
Clone ID Related Accession (Same CDS sequence)	XM_017003428.1 , XM_017003429.2 , XM_017003429.1 , XM_017003428.2
Accession Version	XM_017003429.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGTGT GCCAAAAGGT TGAGGCCTCT GGGTTTGAAT TCACCAGCTT GGTATTTTCA 
GTCTCCTTCC CACCACAACT ATCTGTAAGA GAGCATGCTG CATGGTTGCT GGTAAAACAG 
GAAATGGGAA AGCAGAGTCT GTCGCTGGGA AGAGATGATA TAGTTCAGCT AAAAGAAGCC 
TACAAATGGA TAACTCACCC CCTGTTTTCA GAGGAACTGG GTGTTCCCAT TGATGGAAAG 
AGCTTGTTTG AAGTGAGTGT GGTCTTTGCT CACCCAGAAA CAGTTGAGGA TTGCCACTTC 
CTAGCTGCGA TATGCCCAGA TTGTTTTAAG CCAGCCAAAA ACAAACAGTC TGTATTCACT 
AGAATGGCAG TTATGAAAGC CTTGAATAAG ATAAAGGAAG AGGATTTCCT TAAGCAGTTT 
CCTTGTCCTC CAAACTCACC AAAGGCTGTA TGCGCTGTTC TTGAAATTGA ATGTGCTCAT 
GGTGCTGTTT TTGTGGCTGG GAGATATAAT AAATACTCCA GGAATCTACC ACAAACTCCT 
TGGATAATTG ATGGAGAAAG GAAGCTGGAA TCTTCAGTGG AAGAATTAAT TTCAGATCAT 
CTGTTGGCAG TATTTAAAGC AGAGAGTTTT AATTTTTCAT CCTCTGGAAG AGAAGATGTA 
GATGTGAGAA CATTAGGAAA TGGAAGGCCC TTTGCAATTG AGCTGGTGAA TCCTCATAGA 
GTACATTTCA CTTCACAAGA AATTAAGGAA CTTCAGCAGA AAATTAATAA CTCATCTAAC 
AAAATCCAAG TACGTGACTT GCAGCTTGTC ACAAGAGAGG CAATAGGACA TATGAAAGAA 
GGTGAAGAAG AAAAGACAAA GACCTACAGT GCCTTAATTT GGACAAATAA AGCGATACAG 
AAGAAAGACA TTGAATTCCT AAATGACATA AAGGACTTAA AAATCGACCA GAAAACACCT 
TTGCGCGTCC TTCACCGAAG GCCCCTGGCT GTGCGAGCTC GCGTCATTCA CTTCATGGAG 
ACACAGTACG TGGATGAGCA CCACTTCCGC CTCCACTTGA AAACTCAGGC TGGCACCTAC 
ATTAAAGAGT TTGTACATGG AGACTTCGGG AGAACCAAGC CAAACATTGG GTCCCTGATG 
AATGTGACTG CAGACATTCT GGAGCTGGAT GTTGAGTCTG TAGATGTTGA CTGGCCACCT 
GCTCTGGATG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858918.1
CDS	88..1422
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_017003429.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridylate synthase 10 (PUS10), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003429.1

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGTGT GCCAAAAGGT TGAGGCCTCT GGGTTTGAAT TCACCAGCTT GGTATTTTCA 
GTCTCCTTCC CACCACAACT ATCTGTAAGA GAGCATGCTG CATGGTTGCT GGTAAAACAG 
GAAATGGGAA AGCAGAGTCT GTCGCTGGGA AGAGATGATA TAGTTCAGCT AAAAGAAGCC 
TACAAATGGA TAACTCACCC CCTGTTTTCA GAGGAACTGG GTGTTCCCAT TGATGGAAAG 
AGCTTGTTTG AAGTGAGTGT GGTCTTTGCT CACCCAGAAA CAGTTGAGGA TTGCCACTTC 
CTAGCTGCGA TATGCCCAGA TTGTTTTAAG CCAGCCAAAA ACAAACAGTC TGTATTCACT 
AGAATGGCAG TTATGAAAGC CTTGAATAAG ATAAAGGAAG AGGATTTCCT TAAGCAGTTT 
CCTTGTCCTC CAAACTCACC AAAGGCTGTA TGCGCTGTTC TTGAAATTGA ATGTGCTCAT 
GGTGCTGTTT TTGTGGCTGG GAGATATAAT AAATACTCCA GGAATCTACC ACAAACTCCT 
TGGATAATTG ATGGAGAAAG GAAGCTGGAA TCTTCAGTGG AAGAATTAAT TTCAGATCAT 
CTGTTGGCAG TATTTAAAGC AGAGAGTTTT AATTTTTCAT CCTCTGGAAG AGAAGATGTA 
GATGTGAGAA CATTAGGAAA TGGAAGGCCC TTTGCAATTG AGCTGGTGAA TCCTCATAGA 
GTACATTTCA CTTCACAAGA AATTAAGGAA CTTCAGCAGA AAATTAATAA CTCATCTAAC 
AAAATCCAAG TACGTGACTT GCAGCTTGTC ACAAGAGAGG CAATAGGACA TATGAAAGAA 
GGTGAAGAAG AAAAGACAAA GACCTACAGT GCCTTAATTT GGACAAATAA AGCGATACAG 
AAGAAAGACA TTGAATTCCT AAATGACATA AAGGACTTAA AAATCGACCA GAAAACACCT 
TTGCGCGTCC TTCACCGAAG GCCCCTGGCT GTGCGAGCTC GCGTCATTCA CTTCATGGAG 
ACACAGTACG TGGATGAGCA CCACTTCCGC CTCCACTTGA AAACTCAGGC TGGCACCTAC 
ATTAAAGAGT TTGTACATGG AGACTTCGGG AGAACCAAGC CAAACATTGG GTCCCTGATG 
AATGTGACTG CAGACATTCT GGAGCTGGAT GTTGAGTCTG TAGATGTTGA CTGGCCACCT 
GCTCTGGATG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PUS10 ( XM_017003428.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858917.1 Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu89058
Clone ID Related Accession (Same CDS sequence)	XM_017003428.1 , XM_017003429.2 , XM_017003429.1 , XM_017003428.2
Accession Version	XM_017003428.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	putative tRNA pseudouridine synthase Pus10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017003428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGTGT GCCAAAAGGT TGAGGCCTCT GGGTTTGAAT TCACCAGCTT GGTATTTTCA 
GTCTCCTTCC CACCACAACT ATCTGTAAGA GAGCATGCTG CATGGTTGCT GGTAAAACAG 
GAAATGGGAA AGCAGAGTCT GTCGCTGGGA AGAGATGATA TAGTTCAGCT AAAAGAAGCC 
TACAAATGGA TAACTCACCC CCTGTTTTCA GAGGAACTGG GTGTTCCCAT TGATGGAAAG 
AGCTTGTTTG AAGTGAGTGT GGTCTTTGCT CACCCAGAAA CAGTTGAGGA TTGCCACTTC 
CTAGCTGCGA TATGCCCAGA TTGTTTTAAG CCAGCCAAAA ACAAACAGTC TGTATTCACT 
AGAATGGCAG TTATGAAAGC CTTGAATAAG ATAAAGGAAG AGGATTTCCT TAAGCAGTTT 
CCTTGTCCTC CAAACTCACC AAAGGCTGTA TGCGCTGTTC TTGAAATTGA ATGTGCTCAT 
GGTGCTGTTT TTGTGGCTGG GAGATATAAT AAATACTCCA GGAATCTACC ACAAACTCCT 
TGGATAATTG ATGGAGAAAG GAAGCTGGAA TCTTCAGTGG AAGAATTAAT TTCAGATCAT 
CTGTTGGCAG TATTTAAAGC AGAGAGTTTT AATTTTTCAT CCTCTGGAAG AGAAGATGTA 
GATGTGAGAA CATTAGGAAA TGGAAGGCCC TTTGCAATTG AGCTGGTGAA TCCTCATAGA 
GTACATTTCA CTTCACAAGA AATTAAGGAA CTTCAGCAGA AAATTAATAA CTCATCTAAC 
AAAATCCAAG TACGTGACTT GCAGCTTGTC ACAAGAGAGG CAATAGGACA TATGAAAGAA 
GGTGAAGAAG AAAAGACAAA GACCTACAGT GCCTTAATTT GGACAAATAA AGCGATACAG 
AAGAAAGACA TTGAATTCCT AAATGACATA AAGGACTTAA AAATCGACCA GAAAACACCT 
TTGCGCGTCC TTCACCGAAG GCCCCTGGCT GTGCGAGCTC GCGTCATTCA CTTCATGGAG 
ACACAGTACG TGGATGAGCA CCACTTCCGC CTCCACTTGA AAACTCAGGC TGGCACCTAC 
ATTAAAGAGT TTGTACATGG AGACTTCGGG AGAACCAAGC CAAACATTGG GTCCCTGATG 
AATGTGACTG CAGACATTCT GGAGCTGGAT GTTGAGTCTG TAGATGTTGA CTGGCCACCT 
GCTCTGGATG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858917.1
CDS	185..1519
Translation	MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIVQLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD

Target ORF information:

RefSeq Version	XM_017003428.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pseudouridine synthase 10 (PUS10), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003428.2

ORF Insert Sequence:

ATGTTCCCAC TGACTGAGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAA TACTGGTACT 
TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCTTA CAAACTTCCA 
TACAAGGTGT GCCAAAAGGT TGAGGCCTCT GGGTTTGAAT TCACCAGCTT GGTATTTTCA 
GTCTCCTTCC CACCACAACT ATCTGTAAGA GAGCATGCTG CATGGTTGCT GGTAAAACAG 
GAAATGGGAA AGCAGAGTCT GTCGCTGGGA AGAGATGATA TAGTTCAGCT AAAAGAAGCC 
TACAAATGGA TAACTCACCC CCTGTTTTCA GAGGAACTGG GTGTTCCCAT TGATGGAAAG 
AGCTTGTTTG AAGTGAGTGT GGTCTTTGCT CACCCAGAAA CAGTTGAGGA TTGCCACTTC 
CTAGCTGCGA TATGCCCAGA TTGTTTTAAG CCAGCCAAAA ACAAACAGTC TGTATTCACT 
AGAATGGCAG TTATGAAAGC CTTGAATAAG ATAAAGGAAG AGGATTTCCT TAAGCAGTTT 
CCTTGTCCTC CAAACTCACC AAAGGCTGTA TGCGCTGTTC TTGAAATTGA ATGTGCTCAT 
GGTGCTGTTT TTGTGGCTGG GAGATATAAT AAATACTCCA GGAATCTACC ACAAACTCCT 
TGGATAATTG ATGGAGAAAG GAAGCTGGAA TCTTCAGTGG AAGAATTAAT TTCAGATCAT 
CTGTTGGCAG TATTTAAAGC AGAGAGTTTT AATTTTTCAT CCTCTGGAAG AGAAGATGTA 
GATGTGAGAA CATTAGGAAA TGGAAGGCCC TTTGCAATTG AGCTGGTGAA TCCTCATAGA 
GTACATTTCA CTTCACAAGA AATTAAGGAA CTTCAGCAGA AAATTAATAA CTCATCTAAC 
AAAATCCAAG TACGTGACTT GCAGCTTGTC ACAAGAGAGG CAATAGGACA TATGAAAGAA 
GGTGAAGAAG AAAAGACAAA GACCTACAGT GCCTTAATTT GGACAAATAA AGCGATACAG 
AAGAAAGACA TTGAATTCCT AAATGACATA AAGGACTTAA AAATCGACCA GAAAACACCT 
TTGCGCGTCC TTCACCGAAG GCCCCTGGCT GTGCGAGCTC GCGTCATTCA CTTCATGGAG 
ACACAGTACG TGGATGAGCA CCACTTCCGC CTCCACTTGA AAACTCAGGC TGGCACCTAC 
ATTAAAGAGT TTGTACATGG AGACTTCGGG AGAACCAAGC CAAACATTGG GTCCCTGATG 
AATGTGACTG CAGACATTCT GGAGCTGGAT GTTGAGTCTG TAGATGTTGA CTGGCCACCT 
GCTCTGGATG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reciprocal amplification of caspase-3 activity by nuclear export of a putative human RNA-modifying protein, PUS10 during TRAIL-induced apoptosis.
Cell death & disease8(10)e3093(2017 Oct)
Jana S,Hsieh AC,Gupta R

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease.
Nature491(7422)119-24(2012 Nov)
Jostins L,Ripke S,Weersma RK,Duerr RH,McGovern DP,Hui KY,Lee JC,Schumm LP,Sharma Y,Anderson CA,Essers J,Mitrovic M,Ning K,Cleynen I,Theatre E,Spain SL,Raychaudhuri S,Goyette P,Wei Z,Abraham C,Achkar JP,Ahmad T,Amininejad L,Ananthakrishnan AN,Andersen V,Andrews JM,Baidoo L,Balschun T,Bampton PA,Bitton A,Boucher G,Brand S,B?ning C,Cohain A,Cichon S,D'Amato M,De Jong D,Devaney KL,Dubinsky M,Edwards C,Ellinghaus D,Ferguson LR,Franchimont D,Fransen K,Gearry R,Georges M,Gieger C,Glas J,Haritunians T,Hart A,Hawkey C,Hedl M,Hu X,Karlsen TH,Kupcinskas L,Kugathasan S,Latiano A,Laukens D,Lawrance IC,Lees CW,Louis E,Mahy G,Mansfield J,Morgan AR,Mowat C,Newman W,Palmieri O,Ponsioen CY,Potocnik U,Prescott NJ,Regueiro M,Rotter JI,Russell RK,Sanderson JD,Sans M,Satsangi J,Schreiber S,Simms LA,Sventoraityte J,Targan SR,Taylor KD,Tremelling M,Verspaget HW,De Vos M,Wijmenga C,Wilson DC,Winkelmann J,Xavier RJ,Zeissig S,Zhang B,Zhang CK,Zhao H,,Silverberg MS,Annese V,Hakonarson H,Brant SR,Radford-Smith G,Mathew CG,Rioux JD,Schadt EE,Daly MJ,Franke A,Parkes M,Vermeire S,Barrett JC,Cho JH

A genome-wide scan of Ashkenazi Jewish Crohn's disease suggests novel susceptibility loci.
PLoS genetics8(3)e1002559(2012)
Kenny EE,Pe'er I,Karban A,Ozelius L,Mitchell AA,Ng SM,Erazo M,Ostrer H,Abraham C,Abreu MT,Atzmon G,Barzilai N,Brant SR,Bressman S,Burns ER,Chowers Y,Clark LN,Darvasi A,Doheny D,Duerr RH,Eliakim R,Giladi N,Gregersen PK,Hakonarson H,Jones MR,Marder K,McGovern DP,Mulle J,Orr-Urtreger A,Proctor DD,Pulver A,Rotter JI,Silverberg MS,Ullman T,Warren ST,Waterman M,Zhang W,Bergman A,Mayer L,Katz S,Desnick RJ,Cho JH,Peter I

A meta-analysis of genome-wide association scans identifies IL18RAP, PTPN2, TAGAP, and PUS10 as shared risk loci for Crohn's disease and celiac disease.
PLoS genetics7(1)e1001283(2011 Jan)
Festen EA,Goyette P,Green T,Boucher G,Beauchamp C,Trynka G,Dubois PC,Lagac? C,Stokkers PC,Hommes DW,Barisani D,Palmieri O,Annese V,van Heel DA,Weersma RK,Daly MJ,Wijmenga C,Rioux JD

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PUS10 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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