AIFM3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AIFM3
Entrez Gene ID	150209
Full Name	apoptosis inducing factor, mitochondria associated 3
Synonyms	AIFL
General protein information	Preferred Names apoptosis inducing factor, mitochondria associated 3 Names apoptosis-inducing factor 3 apoptosis-inducing factor like apoptosis-inducing factor, mitochondrion-associated, 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q11.21
Disorder MIM:	617298

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018060.2	NP_001018070.1	apoptosis-inducing factor 3 isoform 2
NM_001146288.2	NP_001139760.1	apoptosis-inducing factor 3 isoform 3
NM_001146288.1	NP_001139760.1	apoptosis-inducing factor 3 isoform 3
NM_144704.3	NP_653305.1	apoptosis-inducing factor 3 isoform 1
NM_144704.2	NP_653305.1	apoptosis-inducing factor 3 isoform 1
NM_001018060.3	NP_001018070.1	apoptosis-inducing factor 3 isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AIFM3	XP_005636718.1
Mus musculus (house mouse)	Aifm3	NP_780387.2
Neurospora crassa	NCU05850	XP_959841.2
Oryza sativa (rice)	Os08g0151800	NP_001061002.1
	AIFM3	XP_002798330.1
Bos taurus (cattle)	AIFM3	NP_001039746.2
Rattus norvegicus (Norway rat)	Aifm3	NP_001013999.2
Danio rerio (zebrafish)	LOC100150876	NP_001121885.1
Arabidopsis thaliana (thale cress)	MDAR6	NP_564818.1
Homo sapiens (human)	AIFM3	NP_653305.1
Gallus gallus (chicken)	AIFM3	XP_004934486.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003904	XP_003436656.1
Schizosaccharomyces pombe (fission yeast)	aif1	XP_001713117.2
Pan troglodytes (chimpanzee)	AIFM3	XP_003317162.1
Magnaporthe oryzae (rice blast fungus)	MGG_08262	XP_003715823.1
Xenopus tropicalis (tropical clawed frog)	LOC100493696	XP_002931767.2

Gene Ontology
Bibliography

Related articles in PubMed

High expression of apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) in human cholangiocarcinoma.
Chua-On D, Proungvitaya T, Techasen A, Limpaiboon T, Roytrakul S, Wongkham S, Wongkham C, Somintara O, Sungkhamanon S, Proungvitaya S
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(10)13659-13667(2016 Oct)

Downregulation of miR-210 expression inhibits proliferation, induces apoptosis and enhances radiosensitivity in hypoxic human hepatoma cells in vitro.
Yang W, Sun T, Cao J, Liu F, Tian Y, Zhu W
Experimental cell research318(8)944-54(2012 May)

[Expression of AIF and CGRP markers in pineal gland and thymus during aging].
Lin'kova NS, Katanugina AS, Khavinson VKh
Advances in gerontology = Uspekhi gerontologii24(4)601-5(2011)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Upregulated expression of AIFM3 is associated with cholangiocarcinoma.
Title: High expression of apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) in human cholangiocarcinoma.

AIFM3 is a direct target of miR-210 in human hepatoma cells.
Title: Downregulation of miR-210 expression inhibits proliferation, induces apoptosis and enhances radiosensitivity in hypoxic human hepatoma cells in vitro.

The expression of apoptosis-inducing factor was identified in pineal gland and thymus, but it did not change with age.
Title: [Expression of AIF and CGRP markers in pineal gland and thymus during aging].

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

H. pylori triggers apoptosis in AGS cells via interaction with death receptors in the plasma membrane, leading to the cleavage of procaspase-8, release of cytochrome c and AIF from mitochondria, and activation of subsequent downstream apoptotic events
Title: H. pylori-induced apoptosis in human gastric cancer cells mediated via the release of apoptosis-inducing factor from mitochondria.

The following AIFM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AIFM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09082	NM_001018060.3 Latest version!	Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu09124	NM_144704.3 Latest version!	Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu31437	NM_001146288.2 Latest version!	Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu31437	NM_001146288.1	Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu09124	NM_144704.2	Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu09082	NM_001018060.2	Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AIFM3 ( NM_001018060.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001018070.1 Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09082
Clone ID Related Accession (Same CDS sequence)	NM_001018060.2 , NM_001018060.3
Accession Version	NM_001018060.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-20
Organism	Homo sapiens(human)
Product	apoptosis-inducing factor 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA396585.1, BC032485.1 and BQ028401.1. On Apr 8, 2009 this sequence version replaced NM_001018060.1. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC032485.1, SRR1660803.163114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 15764604; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGCT GCTTCTCCAA ACCCAAACCA GTGGAGCTCA AGATCGAGGT GGTGCTGCCT 
GAGAAGGAGC GAGGCAAGGA GGAGCTGTCG GCCAGTGGGA AGGGCAGCCC CCGGGCCTAC 
CAGGGCAATG GCACGGCCCG CCACTTCCAC ACGGAGGAGC GCCTGTCCAC CCCTCACCCC 
TACCCCAGCC CTCAGGATTG CGTGGAGGCT GCTGTCTGCC ACGTCAAGGA CCTCGAGAAT 
GGCCAGATGC GGGAAGTGGA GCTGGGCTGG GGGAAGGTGT TGCTGGTGAA GGACAATGGG 
GAGTTCCACG CCCTGGGCCA TAAGTGTCCG CACTACGGCG CACCCCTGGT GAAAGGCGTT 
CTGTCCCGTG GTCGGGTGCG CTGCCCCTGG CACGGCGCCT GCTTCAACAT CAGCACTGGG 
GACCTGGAGG ACTTCCCTGG CCTGGACAGT CTACACAAGT TCCAGGTGAA GATTGAGAAG 
GAGAAGGTGT ACGTCCGGGC CAGCAAGCAG GCCCTACAGC TGCAGCGAAG GACCAAGGTG 
ATGGCCAAGT GTATCTCTCC AAGTGCTGGG TACAGCAGTA GCACCAATGT GCTCATTGTG 
GGTGCAGGTG CAGCTGGCCT GGTGTGTGCA GAGACACTGC GGCAGGAGGG CTTCTCCGAC 
CGGATCGTCC TGTGCACGCT AGACCGGCAC CTTCCCTACG ACCGTCCCAA GCTCAGCAAG 
TCCCTGGACA CACAGCCTGA GCAGCTGGCC CTGAGGCCCA AGGAGTTTTT CCGAGCCTAT 
GGCATCGAGG TGCTCACCGA GGCTCAGGTG GTCACAGTGG ACGTGAGAAC TAAGAAGGTC 
GTGTTCAAGG ATGGCTTCAA GCTGGAGTAC AGCAAGCTGC TGCTGGCACC AGGGAGCAGC 
CCCAAGACTC TGAGCTGCAA AGGCAAAGAA GTGGAGAACG TGTTCACTAT CCGGACGCCA 
GAGGATGCCA ATCGCGTGGT GAGGCTGGCC CGAGGCCGCA ACGTGGTCGT CGTGGGAGCC 
GGCTTCCTGG GGATGGAGGT GGCCGCTTAC CTGACGGAGA AGGCCCACTC TGTGTCTGTG 
GTGGAGCTGG AGGAGACGCC CTTCAGGAGG TTCCTGGGGG AGCGCGTGGG TCGTGCCCTC 
ATGAAGATGT TTGAGAACAA CCGGGTGAAG TTCTACATGC AGACGGAGGT GTCTGAGCTG 
CGGGGCCAGG AGGGAAAGCT GAAGGAGGTT GTGCTGAAGA GCAGCAAGGT CGTGCGGGCT 
GACGTCTGCG TGGTGGGCAT TGGTGCAGTG CCCGCCACAG GCTTCCTGAG GCAAAGCGGC 
ATCGGTTTGG ATTCCCGAGG CTTCATCCCT GTCAACAAGA TGATGCAGAC CAATGTCCCA 
GGCGTGTTTG CAGCTGGCGA TGCTGTCACC TTCCCCCTTG CCTGGAGGAA CAACCGCAAA 
GTGAACATTC CACATTGGCA GATGGCTCAT GCTCAGGGGC GCGTGGCAGC CCAGAACATG 
TTGGCGCAGG AGGCGGAGAT GAGCACTGTG CCCTACCTCT GGACCGCCAT GTTTGGCAAG 
AGCCTGCGCT ACGCGGGCTA CGGAGAAGGC TTCGACGACG TCATCATCCA GGGGGATCTG 
GAGGAGCTGA AGTTTGTGGC TTTTTACACT AAAGGCGACG AGGTGATCGC CGTGGCCAGC 
ATGAACTACG ATCCCATTGT GTCCAAGGTC GCTGAGGTGC TGGCCTCAGG CCGTGCCATC 
CGGAAGCGGG AGGTGGAGAC TGGCGACATG TCCTGGCTTA CGGGGAAAGG ATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018070.1
CDS	219..2015
Translation	MGGCFSKPKPVELKIEVVLPEKERGKEELSASGKGSPRAYQGNGTARHFHTEERLSTPHPYPSPQDCVEAAVCHVKDLENGQMREVELGWGKVLLVKDNGEFHALGHKCPHYGAPLVKGVLSRGRVRCPWHGACFNISTGDLEDFPGLDSLHKFQVKIEKEKVYVRASKQALQLQRRTKVMAKCISPSAGYSSSTNVLIVGAGAAGLVCAETLRQEGFSDRIVLCTLDRHLPYDRPKLSKSLDTQPEQLALRPKEFFRAYGIEVLTEAQVVTVDVRTKKVVFKDGFKLEYSKLLLAPGSSPKTLSCKGKEVENVFTIRTPEDANRVVRLARGRNVVVVGAGFLGMEVAAYLTEKAHSVSVVELEETPFRRFLGERVGRALMKMFENNRVKFYMQTEVSELRGQEGKLKEVVLKSSKVVRADVCVVGIGAVPATGFLRQSGIGLDSRGFIPVNKMMQTNVPGVFAAGDAVTFPLAWRNNRKVNIPHWQMAHAQGRVAAQNMLAQEAEMSTVPYLWTAMFGKSLRYAGYGEGFDDVIIQGDLEELKFVAFYTKGDEVIAVASMNYDPIVSKVAEVLASGRAIRKREVETGDMSWLTGKGS

Target ORF information:

RefSeq Version	NM_001018060.2
Organism	Homo sapiens(human)
Definition	Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018060.2

ORF Insert Sequence:

ATGGGCGGCT GCTTCTCCAA ACCCAAACCA GTGGAGCTCA AGATCGAGGT GGTGCTGCCT 
GAGAAGGAGC GAGGCAAGGA GGAGCTGTCG GCCAGTGGGA AGGGCAGCCC CCGGGCCTAC 
CAGGGCAATG GCACGGCCCG CCACTTCCAC ACGGAGGAGC GCCTGTCCAC CCCTCACCCC 
TACCCCAGCC CTCAGGATTG CGTGGAGGCT GCTGTCTGCC ACGTCAAGGA CCTCGAGAAT 
GGCCAGATGC GGGAAGTGGA GCTGGGCTGG GGGAAGGTGT TGCTGGTGAA GGACAATGGG 
GAGTTCCACG CCCTGGGCCA TAAGTGTCCG CACTACGGCG CACCCCTGGT GAAAGGCGTT 
CTGTCCCGTG GTCGGGTGCG CTGCCCCTGG CACGGCGCCT GCTTCAACAT CAGCACTGGG 
GACCTGGAGG ACTTCCCTGG CCTGGACAGT CTACACAAGT TCCAGGTGAA GATTGAGAAG 
GAGAAGGTGT ACGTCCGGGC CAGCAAGCAG GCCCTACAGC TGCAGCGAAG GACCAAGGTG 
ATGGCCAAGT GTATCTCTCC AAGTGCTGGG TACAGCAGTA GCACCAATGT GCTCATTGTG 
GGTGCAGGTG CAGCTGGCCT GGTGTGTGCA GAGACACTGC GGCAGGAGGG CTTCTCCGAC 
CGGATCGTCC TGTGCACGCT AGACCGGCAC CTTCCCTACG ACCGTCCCAA GCTCAGCAAG 
TCCCTGGACA CACAGCCTGA GCAGCTGGCC CTGAGGCCCA AGGAGTTTTT CCGAGCCTAT 
GGCATCGAGG TGCTCACCGA GGCTCAGGTG GTCACAGTGG ACGTGAGAAC TAAGAAGGTC 
GTGTTCAAGG ATGGCTTCAA GCTGGAGTAC AGCAAGCTGC TGCTGGCACC AGGGAGCAGC 
CCCAAGACTC TGAGCTGCAA AGGCAAAGAA GTGGAGAACG TGTTCACTAT CCGGACGCCA 
GAGGATGCCA ATCGCGTGGT GAGGCTGGCC CGAGGCCGCA ACGTGGTCGT CGTGGGAGCC 
GGCTTCCTGG GGATGGAGGT GGCCGCTTAC CTGACGGAGA AGGCCCACTC TGTGTCTGTG 
GTGGAGCTGG AGGAGACGCC CTTCAGGAGG TTCCTGGGGG AGCGCGTGGG TCGTGCCCTC 
ATGAAGATGT TTGAGAACAA CCGGGTGAAG TTCTACATGC AGACGGAGGT GTCTGAGCTG 
CGGGGCCAGG AGGGAAAGCT GAAGGAGGTT GTGCTGAAGA GCAGCAAGGT CGTGCGGGCT 
GACGTCTGCG TGGTGGGCAT TGGTGCAGTG CCCGCCACAG GCTTCCTGAG GCAAAGCGGC 
ATCGGTTTGG ATTCCCGAGG CTTCATCCCT GTCAACAAGA TGATGCAGAC CAATGTCCCA 
GGCGTGTTTG CAGCTGGCGA TGCTGTCACC TTCCCCCTTG CCTGGAGGAA CAACCGCAAA 
GTGAACATTC CACATTGGCA GATGGCTCAT GCTCAGGGGC GCGTGGCAGC CCAGAACATG 
TTGGCGCAGG AGGCGGAGAT GAGCACTGTG CCCTACCTCT GGACCGCCAT GTTTGGCAAG 
AGCCTGCGCT ACGCGGGCTA CGGAGAAGGC TTCGACGACG TCATCATCCA GGGGGATCTG 
GAGGAGCTGA AGTTTGTGGC TTTTTACACT AAAGGCGACG AGGTGATCGC CGTGGCCAGC 
ATGAACTACG ATCCCATTGT GTCCAAGGTC GCTGAGGTGC TGGCCTCAGG CCGTGCCATC 
CGGAAGCGGG AGGTGGAGAC TGGCGACATG TCCTGGCTTA CGGGGAAAGG ATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AIFM3 ( NM_001018060.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001018070.1 Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09082
Clone ID Related Accession (Same CDS sequence)	NM_001018060.2 , NM_001018060.3
Accession Version	NM_001018060.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	apoptosis-inducing factor 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC002470.17 and KF457368.1. On Jun 2, 2019 this sequence version replaced NM_001018060.2. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC032485.1, SRR1660803.163114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 15764604; reported by MitoCarta RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGCT GCTTCTCCAA ACCCAAACCA GTGGAGCTCA AGATCGAGGT GGTGCTGCCT 
GAGAAGGAGC GAGGCAAGGA GGAGCTGTCG GCCAGTGGGA AGGGCAGCCC CCGGGCCTAC 
CAGGGCAATG GCACGGCCCG CCACTTCCAC ACGGAGGAGC GCCTGTCCAC CCCTCACCCC 
TACCCCAGCC CTCAGGATTG CGTGGAGGCT GCTGTCTGCC ACGTCAAGGA CCTCGAGAAT 
GGCCAGATGC GGGAAGTGGA GCTGGGCTGG GGGAAGGTGT TGCTGGTGAA GGACAATGGG 
GAGTTCCACG CCCTGGGCCA TAAGTGTCCG CACTACGGCG CACCCCTGGT GAAAGGCGTT 
CTGTCCCGTG GTCGGGTGCG CTGCCCCTGG CACGGCGCCT GCTTCAACAT CAGCACTGGG 
GACCTGGAGG ACTTCCCTGG CCTGGACAGT CTACACAAGT TCCAGGTGAA GATTGAGAAG 
GAGAAGGTGT ACGTCCGGGC CAGCAAGCAG GCCCTACAGC TGCAGCGAAG GACCAAGGTG 
ATGGCCAAGT GTATCTCTCC AAGTGCTGGG TACAGCAGTA GCACCAATGT GCTCATTGTG 
GGTGCAGGTG CAGCTGGCCT GGTGTGTGCA GAGACACTGC GGCAGGAGGG CTTCTCCGAC 
CGGATCGTCC TGTGCACGCT AGACCGGCAC CTTCCCTACG ACCGTCCCAA GCTCAGCAAG 
TCCCTGGACA CACAGCCTGA GCAGCTGGCC CTGAGGCCCA AGGAGTTTTT CCGAGCCTAT 
GGCATCGAGG TGCTCACCGA GGCTCAGGTG GTCACAGTGG ACGTGAGAAC TAAGAAGGTC 
GTGTTCAAGG ATGGCTTCAA GCTGGAGTAC AGCAAGCTGC TGCTGGCACC AGGGAGCAGC 
CCCAAGACTC TGAGCTGCAA AGGCAAAGAA GTGGAGAACG TGTTCACTAT CCGGACGCCA 
GAGGATGCCA ATCGCGTGGT GAGGCTGGCC CGAGGCCGCA ACGTGGTCGT CGTGGGAGCC 
GGCTTCCTGG GGATGGAGGT GGCCGCTTAC CTGACGGAGA AGGCCCACTC TGTGTCTGTG 
GTGGAGCTGG AGGAGACGCC CTTCAGGAGG TTCCTGGGGG AGCGCGTGGG TCGTGCCCTC 
ATGAAGATGT TTGAGAACAA CCGGGTGAAG TTCTACATGC AGACGGAGGT GTCTGAGCTG 
CGGGGCCAGG AGGGAAAGCT GAAGGAGGTT GTGCTGAAGA GCAGCAAGGT CGTGCGGGCT 
GACGTCTGCG TGGTGGGCAT TGGTGCAGTG CCCGCCACAG GCTTCCTGAG GCAAAGCGGC 
ATCGGTTTGG ATTCCCGAGG CTTCATCCCT GTCAACAAGA TGATGCAGAC CAATGTCCCA 
GGCGTGTTTG CAGCTGGCGA TGCTGTCACC TTCCCCCTTG CCTGGAGGAA CAACCGCAAA 
GTGAACATTC CACATTGGCA GATGGCTCAT GCTCAGGGGC GCGTGGCAGC CCAGAACATG 
TTGGCGCAGG AGGCGGAGAT GAGCACTGTG CCCTACCTCT GGACCGCCAT GTTTGGCAAG 
AGCCTGCGCT ACGCGGGCTA CGGAGAAGGC TTCGACGACG TCATCATCCA GGGGGATCTG 
GAGGAGCTGA AGTTTGTGGC TTTTTACACT AAAGGCGACG AGGTGATCGC CGTGGCCAGC 
ATGAACTACG ATCCCATTGT GTCCAAGGTC GCTGAGGTGC TGGCCTCAGG CCGTGCCATC 
CGGAAGCGGG AGGTGGAGAC TGGCGACATG TCCTGGCTTA CGGGGAAAGG ATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018070.1
CDS	177..1973
Translation	MGGCFSKPKPVELKIEVVLPEKERGKEELSASGKGSPRAYQGNGTARHFHTEERLSTPHPYPSPQDCVEAAVCHVKDLENGQMREVELGWGKVLLVKDNGEFHALGHKCPHYGAPLVKGVLSRGRVRCPWHGACFNISTGDLEDFPGLDSLHKFQVKIEKEKVYVRASKQALQLQRRTKVMAKCISPSAGYSSSTNVLIVGAGAAGLVCAETLRQEGFSDRIVLCTLDRHLPYDRPKLSKSLDTQPEQLALRPKEFFRAYGIEVLTEAQVVTVDVRTKKVVFKDGFKLEYSKLLLAPGSSPKTLSCKGKEVENVFTIRTPEDANRVVRLARGRNVVVVGAGFLGMEVAAYLTEKAHSVSVVELEETPFRRFLGERVGRALMKMFENNRVKFYMQTEVSELRGQEGKLKEVVLKSSKVVRADVCVVGIGAVPATGFLRQSGIGLDSRGFIPVNKMMQTNVPGVFAAGDAVTFPLAWRNNRKVNIPHWQMAHAQGRVAAQNMLAQEAEMSTVPYLWTAMFGKSLRYAGYGEGFDDVIIQGDLEELKFVAFYTKGDEVIAVASMNYDPIVSKVAEVLASGRAIRKREVETGDMSWLTGKGS

Target ORF information:

RefSeq Version	NM_001018060.3
Organism	Homo sapiens(human)
Definition	Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018060.3

ORF Insert Sequence:

ATGGGCGGCT GCTTCTCCAA ACCCAAACCA GTGGAGCTCA AGATCGAGGT GGTGCTGCCT 
GAGAAGGAGC GAGGCAAGGA GGAGCTGTCG GCCAGTGGGA AGGGCAGCCC CCGGGCCTAC 
CAGGGCAATG GCACGGCCCG CCACTTCCAC ACGGAGGAGC GCCTGTCCAC CCCTCACCCC 
TACCCCAGCC CTCAGGATTG CGTGGAGGCT GCTGTCTGCC ACGTCAAGGA CCTCGAGAAT 
GGCCAGATGC GGGAAGTGGA GCTGGGCTGG GGGAAGGTGT TGCTGGTGAA GGACAATGGG 
GAGTTCCACG CCCTGGGCCA TAAGTGTCCG CACTACGGCG CACCCCTGGT GAAAGGCGTT 
CTGTCCCGTG GTCGGGTGCG CTGCCCCTGG CACGGCGCCT GCTTCAACAT CAGCACTGGG 
GACCTGGAGG ACTTCCCTGG CCTGGACAGT CTACACAAGT TCCAGGTGAA GATTGAGAAG 
GAGAAGGTGT ACGTCCGGGC CAGCAAGCAG GCCCTACAGC TGCAGCGAAG GACCAAGGTG 
ATGGCCAAGT GTATCTCTCC AAGTGCTGGG TACAGCAGTA GCACCAATGT GCTCATTGTG 
GGTGCAGGTG CAGCTGGCCT GGTGTGTGCA GAGACACTGC GGCAGGAGGG CTTCTCCGAC 
CGGATCGTCC TGTGCACGCT AGACCGGCAC CTTCCCTACG ACCGTCCCAA GCTCAGCAAG 
TCCCTGGACA CACAGCCTGA GCAGCTGGCC CTGAGGCCCA AGGAGTTTTT CCGAGCCTAT 
GGCATCGAGG TGCTCACCGA GGCTCAGGTG GTCACAGTGG ACGTGAGAAC TAAGAAGGTC 
GTGTTCAAGG ATGGCTTCAA GCTGGAGTAC AGCAAGCTGC TGCTGGCACC AGGGAGCAGC 
CCCAAGACTC TGAGCTGCAA AGGCAAAGAA GTGGAGAACG TGTTCACTAT CCGGACGCCA 
GAGGATGCCA ATCGCGTGGT GAGGCTGGCC CGAGGCCGCA ACGTGGTCGT CGTGGGAGCC 
GGCTTCCTGG GGATGGAGGT GGCCGCTTAC CTGACGGAGA AGGCCCACTC TGTGTCTGTG 
GTGGAGCTGG AGGAGACGCC CTTCAGGAGG TTCCTGGGGG AGCGCGTGGG TCGTGCCCTC 
ATGAAGATGT TTGAGAACAA CCGGGTGAAG TTCTACATGC AGACGGAGGT GTCTGAGCTG 
CGGGGCCAGG AGGGAAAGCT GAAGGAGGTT GTGCTGAAGA GCAGCAAGGT CGTGCGGGCT 
GACGTCTGCG TGGTGGGCAT TGGTGCAGTG CCCGCCACAG GCTTCCTGAG GCAAAGCGGC 
ATCGGTTTGG ATTCCCGAGG CTTCATCCCT GTCAACAAGA TGATGCAGAC CAATGTCCCA 
GGCGTGTTTG CAGCTGGCGA TGCTGTCACC TTCCCCCTTG CCTGGAGGAA CAACCGCAAA 
GTGAACATTC CACATTGGCA GATGGCTCAT GCTCAGGGGC GCGTGGCAGC CCAGAACATG 
TTGGCGCAGG AGGCGGAGAT GAGCACTGTG CCCTACCTCT GGACCGCCAT GTTTGGCAAG 
AGCCTGCGCT ACGCGGGCTA CGGAGAAGGC TTCGACGACG TCATCATCCA GGGGGATCTG 
GAGGAGCTGA AGTTTGTGGC TTTTTACACT AAAGGCGACG AGGTGATCGC CGTGGCCAGC 
ATGAACTACG ATCCCATTGT GTCCAAGGTC GCTGAGGTGC TGGCCTCAGG CCGTGCCATC 
CGGAAGCGGG AGGTGGAGAC TGGCGACATG TCCTGGCTTA CGGGGAAAGG ATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AIFM3 ( NM_144704.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_653305.1 Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 1, mRNA.

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CloneID	OHu09124
Clone ID Related Accession (Same CDS sequence)	NM_144704.3 , NM_144704.2
Accession Version	NM_144704.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	apoptosis-inducing factor 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC002470.17 and KF457368.1. On Jun 2, 2019 this sequence version replaced NM_144704.2. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR7410570.684606.1, SRR3476690.797760.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 15764604; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGCT GCTTCTCCAA ACCCAAACCA GTGGAGCTCA AGATCGAGGT GGTGCTGCCT 
GAGAAGGAGC GAGGCAAGGA GGAGCTGTCG GCCAGTGGGA AGGGCAGCCC CCGGGCCTAC 
CAGGGCAATG GCACGGCCCG CCACTTCCAC ACGGAGGAGC GCCTGTCCAC CCCTCACCCC 
TACCCCAGCC CTCAGGATTG CGTGGAGGCT GCTGTCTGCC ACGTCAAGGA CCTCGAGAAT 
GGCCAGATGC GGGAAGTGGA GCTGGGCTGG GGGAAGGTGT TGCTGGTGAA GGACAATGGG 
GAGTTCCACG CCCTGGGCCA TAAGTGTCCG CACTACGGCG CACCCCTGGT GAAAGGCGTT 
CTGTCCCGTG GTCGGGTGCG CTGCCCCTGG CACGGCGCCT GCTTCAACAT CAGCACTGGG 
GACCTGGAGG ACTTCCCTGG CCTGGACAGT CTACACAAGT TCCAGGTGAA GATTGAGAAG 
GAGAAGGTGT ACGTCCGGGC CAGCAAGCAG GCCCTACAGC TGCAGCGAAG GACCAAGGTG 
ATGGCCAAGT GTATCTCTCC AAGTGCTGGG TACAGCAGTA GCACCAATGT GCTCATTGTG 
GGTGCAGGTG CAGCTGGCCT GGTGTGTGCA GAGACACTGC GGCAGGAGGG CTTCTCCGAC 
CGGATCGTCC TGTGCACGCT AGACCGGCAC CTTCCCTACG ACCGTCCCAA GCTCAGCAAG 
TCCCTGGACA CACAGCCTGA GCAGCTGGCC CTGAGGCCCA AGGAGTTTTT CCGAGCCTAT 
GGCATCGAGG TGCTCACCGA GGCTCAGGTG GTCACAGTGG ACGTGAGAAC TAAGAAGGTC 
GTGTTCAAGG ATGGCTTCAA GCTGGAGTAC AGCAAGCTGC TGCTGGCACC AGGGAGCAGC 
CCCAAGACTC TGAGCTGCAA AGGCAAAGAA GTGGAGAACG TGTTCACTAT CCGGACGCCA 
GAGGATGCCA ATCGCGTGGT GAGGCTGGCC CGAGGCCGCA ACGTGGTCGT CGTGGGAGCC 
GGCTTCCTGG GGATGGAGGT GGCCGCTTAC CTGACGGAGA AGGCCCACTC TGTGTCTGTG 
GTGGAGCTGG AGGAGACGCC CTTCAGGAGG TTCCTGGGGG AGCGCGTGGG TCGTGCCCTC 
ATGAAGATGT TTGAGAACAA CCGGGTGAAG TTCTACATGC AGACGGAGGT GTCTGAGCTG 
CGGGGCCAGG AGGGAAAGCT GAAGGAGGTT GTGCTGAAGA GCAGCAAGGT CGTGCGGGCT 
GACGTCTGCG TGGTGGGCAT TGGTGCAGTG CCCGCCACAG GCTTCCTGAG GCAAAGCGGC 
ATCGGTTTGG ATTCCCGAGG CTTCATCCCT GTCAACAAGA TGATGCAGAC CAATGTCCCA 
GGCGTGTTTG CAGCTGGCGA TGCTGTCACC TTCCCCCTTG CCTGGAGGAA CAACCGCAAA 
GTGAACATTC CACATTGGCA GATGGCTCAT GCTCAGGGGC GCGTGGCAGC CCAGAACATG 
TTGGCGCAGG AGGCGGAGAT GAGCACTGTG CCCTACCTCT GGACCGCCAT GTTTGGCAAG 
AGCCTGCGCT ACGCGGGCTA CGGAGAAGGC TTCGACGACG TCATCATCCA GGGGGATCTG 
GAGGAGCTGA AGTTTGTGGC TTTTTACACT AAAGGCGACG AGGTGATCGC CGTGGCCAGC 
ATGAACTACG ATCCCATTGT GTCCAAGGTC GCTGAGGTGC TGGCCTCAGG CCGTGCCATC 
CGGAAGCGGG AGGTGGAGCT GTTTGTGCTG CACAGCAAGA CTGGCGACAT GTCCTGGCTT 
ACGGGGAAAG GATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653305.1
CDS	177..1994
Translation	MGGCFSKPKPVELKIEVVLPEKERGKEELSASGKGSPRAYQGNGTARHFHTEERLSTPHPYPSPQDCVEAAVCHVKDLENGQMREVELGWGKVLLVKDNGEFHALGHKCPHYGAPLVKGVLSRGRVRCPWHGACFNISTGDLEDFPGLDSLHKFQVKIEKEKVYVRASKQALQLQRRTKVMAKCISPSAGYSSSTNVLIVGAGAAGLVCAETLRQEGFSDRIVLCTLDRHLPYDRPKLSKSLDTQPEQLALRPKEFFRAYGIEVLTEAQVVTVDVRTKKVVFKDGFKLEYSKLLLAPGSSPKTLSCKGKEVENVFTIRTPEDANRVVRLARGRNVVVVGAGFLGMEVAAYLTEKAHSVSVVELEETPFRRFLGERVGRALMKMFENNRVKFYMQTEVSELRGQEGKLKEVVLKSSKVVRADVCVVGIGAVPATGFLRQSGIGLDSRGFIPVNKMMQTNVPGVFAAGDAVTFPLAWRNNRKVNIPHWQMAHAQGRVAAQNMLAQEAEMSTVPYLWTAMFGKSLRYAGYGEGFDDVIIQGDLEELKFVAFYTKGDEVIAVASMNYDPIVSKVAEVLASGRAIRKREVELFVLHSKTGDMSWLTGKGS

Target ORF information:

RefSeq Version	NM_144704.3
Organism	Homo sapiens(human)
Definition	Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144704.3

ORF Insert Sequence:

ATGGGCGGCT GCTTCTCCAA ACCCAAACCA GTGGAGCTCA AGATCGAGGT GGTGCTGCCT 
GAGAAGGAGC GAGGCAAGGA GGAGCTGTCG GCCAGTGGGA AGGGCAGCCC CCGGGCCTAC 
CAGGGCAATG GCACGGCCCG CCACTTCCAC ACGGAGGAGC GCCTGTCCAC CCCTCACCCC 
TACCCCAGCC CTCAGGATTG CGTGGAGGCT GCTGTCTGCC ACGTCAAGGA CCTCGAGAAT 
GGCCAGATGC GGGAAGTGGA GCTGGGCTGG GGGAAGGTGT TGCTGGTGAA GGACAATGGG 
GAGTTCCACG CCCTGGGCCA TAAGTGTCCG CACTACGGCG CACCCCTGGT GAAAGGCGTT 
CTGTCCCGTG GTCGGGTGCG CTGCCCCTGG CACGGCGCCT GCTTCAACAT CAGCACTGGG 
GACCTGGAGG ACTTCCCTGG CCTGGACAGT CTACACAAGT TCCAGGTGAA GATTGAGAAG 
GAGAAGGTGT ACGTCCGGGC CAGCAAGCAG GCCCTACAGC TGCAGCGAAG GACCAAGGTG 
ATGGCCAAGT GTATCTCTCC AAGTGCTGGG TACAGCAGTA GCACCAATGT GCTCATTGTG 
GGTGCAGGTG CAGCTGGCCT GGTGTGTGCA GAGACACTGC GGCAGGAGGG CTTCTCCGAC 
CGGATCGTCC TGTGCACGCT AGACCGGCAC CTTCCCTACG ACCGTCCCAA GCTCAGCAAG 
TCCCTGGACA CACAGCCTGA GCAGCTGGCC CTGAGGCCCA AGGAGTTTTT CCGAGCCTAT 
GGCATCGAGG TGCTCACCGA GGCTCAGGTG GTCACAGTGG ACGTGAGAAC TAAGAAGGTC 
GTGTTCAAGG ATGGCTTCAA GCTGGAGTAC AGCAAGCTGC TGCTGGCACC AGGGAGCAGC 
CCCAAGACTC TGAGCTGCAA AGGCAAAGAA GTGGAGAACG TGTTCACTAT CCGGACGCCA 
GAGGATGCCA ATCGCGTGGT GAGGCTGGCC CGAGGCCGCA ACGTGGTCGT CGTGGGAGCC 
GGCTTCCTGG GGATGGAGGT GGCCGCTTAC CTGACGGAGA AGGCCCACTC TGTGTCTGTG 
GTGGAGCTGG AGGAGACGCC CTTCAGGAGG TTCCTGGGGG AGCGCGTGGG TCGTGCCCTC 
ATGAAGATGT TTGAGAACAA CCGGGTGAAG TTCTACATGC AGACGGAGGT GTCTGAGCTG 
CGGGGCCAGG AGGGAAAGCT GAAGGAGGTT GTGCTGAAGA GCAGCAAGGT CGTGCGGGCT 
GACGTCTGCG TGGTGGGCAT TGGTGCAGTG CCCGCCACAG GCTTCCTGAG GCAAAGCGGC 
ATCGGTTTGG ATTCCCGAGG CTTCATCCCT GTCAACAAGA TGATGCAGAC CAATGTCCCA 
GGCGTGTTTG CAGCTGGCGA TGCTGTCACC TTCCCCCTTG CCTGGAGGAA CAACCGCAAA 
GTGAACATTC CACATTGGCA GATGGCTCAT GCTCAGGGGC GCGTGGCAGC CCAGAACATG 
TTGGCGCAGG AGGCGGAGAT GAGCACTGTG CCCTACCTCT GGACCGCCAT GTTTGGCAAG 
AGCCTGCGCT ACGCGGGCTA CGGAGAAGGC TTCGACGACG TCATCATCCA GGGGGATCTG 
GAGGAGCTGA AGTTTGTGGC TTTTTACACT AAAGGCGACG AGGTGATCGC CGTGGCCAGC 
ATGAACTACG ATCCCATTGT GTCCAAGGTC GCTGAGGTGC TGGCCTCAGG CCGTGCCATC 
CGGAAGCGGG AGGTGGAGCT GTTTGTGCTG CACAGCAAGA CTGGCGACAT GTCCTGGCTT 
ACGGGGAAAG GATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AIFM3 ( NM_144704.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_653305.1 Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 1, mRNA.

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CloneID	OHu09124
Clone ID Related Accession (Same CDS sequence)	NM_144704.3 , NM_144704.2
Accession Version	NM_144704.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-20
Organism	Homo sapiens(human)
Product	apoptosis-inducing factor 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA396585.1, BC032485.1, AK055035.1 and BQ028401.1. On Apr 8, 2009 this sequence version replaced NM_144704.1. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK055035.1, SRR1803613.183462.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 15764604; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGCT GCTTCTCCAA ACCCAAACCA GTGGAGCTCA AGATCGAGGT GGTGCTGCCT 
GAGAAGGAGC GAGGCAAGGA GGAGCTGTCG GCCAGTGGGA AGGGCAGCCC CCGGGCCTAC 
CAGGGCAATG GCACGGCCCG CCACTTCCAC ACGGAGGAGC GCCTGTCCAC CCCTCACCCC 
TACCCCAGCC CTCAGGATTG CGTGGAGGCT GCTGTCTGCC ACGTCAAGGA CCTCGAGAAT 
GGCCAGATGC GGGAAGTGGA GCTGGGCTGG GGGAAGGTGT TGCTGGTGAA GGACAATGGG 
GAGTTCCACG CCCTGGGCCA TAAGTGTCCG CACTACGGCG CACCCCTGGT GAAAGGCGTT 
CTGTCCCGTG GTCGGGTGCG CTGCCCCTGG CACGGCGCCT GCTTCAACAT CAGCACTGGG 
GACCTGGAGG ACTTCCCTGG CCTGGACAGT CTACACAAGT TCCAGGTGAA GATTGAGAAG 
GAGAAGGTGT ACGTCCGGGC CAGCAAGCAG GCCCTACAGC TGCAGCGAAG GACCAAGGTG 
ATGGCCAAGT GTATCTCTCC AAGTGCTGGG TACAGCAGTA GCACCAATGT GCTCATTGTG 
GGTGCAGGTG CAGCTGGCCT GGTGTGTGCA GAGACACTGC GGCAGGAGGG CTTCTCCGAC 
CGGATCGTCC TGTGCACGCT AGACCGGCAC CTTCCCTACG ACCGTCCCAA GCTCAGCAAG 
TCCCTGGACA CACAGCCTGA GCAGCTGGCC CTGAGGCCCA AGGAGTTTTT CCGAGCCTAT 
GGCATCGAGG TGCTCACCGA GGCTCAGGTG GTCACAGTGG ACGTGAGAAC TAAGAAGGTC 
GTGTTCAAGG ATGGCTTCAA GCTGGAGTAC AGCAAGCTGC TGCTGGCACC AGGGAGCAGC 
CCCAAGACTC TGAGCTGCAA AGGCAAAGAA GTGGAGAACG TGTTCACTAT CCGGACGCCA 
GAGGATGCCA ATCGCGTGGT GAGGCTGGCC CGAGGCCGCA ACGTGGTCGT CGTGGGAGCC 
GGCTTCCTGG GGATGGAGGT GGCCGCTTAC CTGACGGAGA AGGCCCACTC TGTGTCTGTG 
GTGGAGCTGG AGGAGACGCC CTTCAGGAGG TTCCTGGGGG AGCGCGTGGG TCGTGCCCTC 
ATGAAGATGT TTGAGAACAA CCGGGTGAAG TTCTACATGC AGACGGAGGT GTCTGAGCTG 
CGGGGCCAGG AGGGAAAGCT GAAGGAGGTT GTGCTGAAGA GCAGCAAGGT CGTGCGGGCT 
GACGTCTGCG TGGTGGGCAT TGGTGCAGTG CCCGCCACAG GCTTCCTGAG GCAAAGCGGC 
ATCGGTTTGG ATTCCCGAGG CTTCATCCCT GTCAACAAGA TGATGCAGAC CAATGTCCCA 
GGCGTGTTTG CAGCTGGCGA TGCTGTCACC TTCCCCCTTG CCTGGAGGAA CAACCGCAAA 
GTGAACATTC CACATTGGCA GATGGCTCAT GCTCAGGGGC GCGTGGCAGC CCAGAACATG 
TTGGCGCAGG AGGCGGAGAT GAGCACTGTG CCCTACCTCT GGACCGCCAT GTTTGGCAAG 
AGCCTGCGCT ACGCGGGCTA CGGAGAAGGC TTCGACGACG TCATCATCCA GGGGGATCTG 
GAGGAGCTGA AGTTTGTGGC TTTTTACACT AAAGGCGACG AGGTGATCGC CGTGGCCAGC 
ATGAACTACG ATCCCATTGT GTCCAAGGTC GCTGAGGTGC TGGCCTCAGG CCGTGCCATC 
CGGAAGCGGG AGGTGGAGCT GTTTGTGCTG CACAGCAAGA CTGGCGACAT GTCCTGGCTT 
ACGGGGAAAG GATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653305.1
CDS	219..2036
Translation	MGGCFSKPKPVELKIEVVLPEKERGKEELSASGKGSPRAYQGNGTARHFHTEERLSTPHPYPSPQDCVEAAVCHVKDLENGQMREVELGWGKVLLVKDNGEFHALGHKCPHYGAPLVKGVLSRGRVRCPWHGACFNISTGDLEDFPGLDSLHKFQVKIEKEKVYVRASKQALQLQRRTKVMAKCISPSAGYSSSTNVLIVGAGAAGLVCAETLRQEGFSDRIVLCTLDRHLPYDRPKLSKSLDTQPEQLALRPKEFFRAYGIEVLTEAQVVTVDVRTKKVVFKDGFKLEYSKLLLAPGSSPKTLSCKGKEVENVFTIRTPEDANRVVRLARGRNVVVVGAGFLGMEVAAYLTEKAHSVSVVELEETPFRRFLGERVGRALMKMFENNRVKFYMQTEVSELRGQEGKLKEVVLKSSKVVRADVCVVGIGAVPATGFLRQSGIGLDSRGFIPVNKMMQTNVPGVFAAGDAVTFPLAWRNNRKVNIPHWQMAHAQGRVAAQNMLAQEAEMSTVPYLWTAMFGKSLRYAGYGEGFDDVIIQGDLEELKFVAFYTKGDEVIAVASMNYDPIVSKVAEVLASGRAIRKREVELFVLHSKTGDMSWLTGKGS

Target ORF information:

RefSeq Version	NM_144704.2
Organism	Homo sapiens(human)
Definition	Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144704.2

ORF Insert Sequence:

ATGGGCGGCT GCTTCTCCAA ACCCAAACCA GTGGAGCTCA AGATCGAGGT GGTGCTGCCT 
GAGAAGGAGC GAGGCAAGGA GGAGCTGTCG GCCAGTGGGA AGGGCAGCCC CCGGGCCTAC 
CAGGGCAATG GCACGGCCCG CCACTTCCAC ACGGAGGAGC GCCTGTCCAC CCCTCACCCC 
TACCCCAGCC CTCAGGATTG CGTGGAGGCT GCTGTCTGCC ACGTCAAGGA CCTCGAGAAT 
GGCCAGATGC GGGAAGTGGA GCTGGGCTGG GGGAAGGTGT TGCTGGTGAA GGACAATGGG 
GAGTTCCACG CCCTGGGCCA TAAGTGTCCG CACTACGGCG CACCCCTGGT GAAAGGCGTT 
CTGTCCCGTG GTCGGGTGCG CTGCCCCTGG CACGGCGCCT GCTTCAACAT CAGCACTGGG 
GACCTGGAGG ACTTCCCTGG CCTGGACAGT CTACACAAGT TCCAGGTGAA GATTGAGAAG 
GAGAAGGTGT ACGTCCGGGC CAGCAAGCAG GCCCTACAGC TGCAGCGAAG GACCAAGGTG 
ATGGCCAAGT GTATCTCTCC AAGTGCTGGG TACAGCAGTA GCACCAATGT GCTCATTGTG 
GGTGCAGGTG CAGCTGGCCT GGTGTGTGCA GAGACACTGC GGCAGGAGGG CTTCTCCGAC 
CGGATCGTCC TGTGCACGCT AGACCGGCAC CTTCCCTACG ACCGTCCCAA GCTCAGCAAG 
TCCCTGGACA CACAGCCTGA GCAGCTGGCC CTGAGGCCCA AGGAGTTTTT CCGAGCCTAT 
GGCATCGAGG TGCTCACCGA GGCTCAGGTG GTCACAGTGG ACGTGAGAAC TAAGAAGGTC 
GTGTTCAAGG ATGGCTTCAA GCTGGAGTAC AGCAAGCTGC TGCTGGCACC AGGGAGCAGC 
CCCAAGACTC TGAGCTGCAA AGGCAAAGAA GTGGAGAACG TGTTCACTAT CCGGACGCCA 
GAGGATGCCA ATCGCGTGGT GAGGCTGGCC CGAGGCCGCA ACGTGGTCGT CGTGGGAGCC 
GGCTTCCTGG GGATGGAGGT GGCCGCTTAC CTGACGGAGA AGGCCCACTC TGTGTCTGTG 
GTGGAGCTGG AGGAGACGCC CTTCAGGAGG TTCCTGGGGG AGCGCGTGGG TCGTGCCCTC 
ATGAAGATGT TTGAGAACAA CCGGGTGAAG TTCTACATGC AGACGGAGGT GTCTGAGCTG 
CGGGGCCAGG AGGGAAAGCT GAAGGAGGTT GTGCTGAAGA GCAGCAAGGT CGTGCGGGCT 
GACGTCTGCG TGGTGGGCAT TGGTGCAGTG CCCGCCACAG GCTTCCTGAG GCAAAGCGGC 
ATCGGTTTGG ATTCCCGAGG CTTCATCCCT GTCAACAAGA TGATGCAGAC CAATGTCCCA 
GGCGTGTTTG CAGCTGGCGA TGCTGTCACC TTCCCCCTTG CCTGGAGGAA CAACCGCAAA 
GTGAACATTC CACATTGGCA GATGGCTCAT GCTCAGGGGC GCGTGGCAGC CCAGAACATG 
TTGGCGCAGG AGGCGGAGAT GAGCACTGTG CCCTACCTCT GGACCGCCAT GTTTGGCAAG 
AGCCTGCGCT ACGCGGGCTA CGGAGAAGGC TTCGACGACG TCATCATCCA GGGGGATCTG 
GAGGAGCTGA AGTTTGTGGC TTTTTACACT AAAGGCGACG AGGTGATCGC CGTGGCCAGC 
ATGAACTACG ATCCCATTGT GTCCAAGGTC GCTGAGGTGC TGGCCTCAGG CCGTGCCATC 
CGGAAGCGGG AGGTGGAGCT GTTTGTGCTG CACAGCAAGA CTGGCGACAT GTCCTGGCTT 
ACGGGGAAAG GATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AIFM3 ( NM_001146288.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139760.1 Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 3, mRNA.

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CloneID	OHu31437
Clone ID Related Accession (Same CDS sequence)	NM_001146288.2 , NM_001146288.1
Accession Version	NM_001146288.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	apoptosis-inducing factor 3 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC002470.17 and KF457368.1. On Jun 2, 2019 this sequence version replaced NM_001146288.1. Transcript Variant: This variant (3) differs in the 5' UTR and has multiple coding region differences compared to variant 1. The encoded isoform (3) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK094844.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 15764604; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGCT GCTTCTCCAA ACCCAAACCA GGCGCTGCCT TGCCCACAGT GGAGCTCAAG 
ATCGAGGTGG TGCTGCCTGA GAAGGAGCGA GGCAAGGAGG AGCTGTCGGC CAGTGGGAAG 
GGCAGCCCCC GGGCCTACCA GGGCAATGGC ACGGCCCGCC ACTTCCACAC GGAGGAGCGC 
CTGTCCACCC CTCACCCCTA CCCCAGCCCT CAGGATTGCG TGGAGGCTGC TGTCTGCCAC 
GTCAAGGACC TCGAGAATGG CCAGATGCGG GAAGTGGAGC TGGGCTGGGG GAAGGTGTTG 
CTGGTGAAGG ACAATGGGGA GTTCCACGCC CTGGGCCATA AGTGTCCGCA CTACGGCGCA 
CCCCTGGTGA AAGGCGTTCT GTCCCGTGGT CGGGTGCGCT GCCCCTGGCA CGGCGCCTGC 
TTCAACATCA GCACTGGGGA CCTGGAGGAC TTCCCTGGCC TGGACAGTCT ACACAAGTTC 
CAGGTGAAGA TTGAGAAGGA GAAGGTGTAC GTCCGGGCCA GCAAGCAGGC CCTACAGCTG 
CAGCGAAGGA CCAAGGTGAT GGCCAAGTGT ATCTCTCCAA GTGCTGGGTA CAGCAGTAGC 
ACCAATGTGC TCATTGTGGG TGCAGGTGCA GCTGGCCTGG TGTGTGCAGA GACACTGCGG 
CAGGAGGGCT TCTCCGACCG GATCGTCCTG TGCACGCTAG ACCGGCACCT TCCCTACGAC 
CGTCCCAAGC TCAGCAAGTC CCTGGACACA CAGCCTGAGC AGCTGGCCCT GAGGCCCAAG 
GAGTTTTTCC GAGCCTATGG CATCGAGGTG CTCACCGAGG CTCAGGTGGT CACAGTGGAC 
GTGAGAACTA AGAAGGTCGT GTTCAAGGAT GGCTTCAAGC TGGAGTACAG CAAGCTGCTG 
CTGGCACCAG GGAGCAGCCC CAAGACTCTG AGCTGCAAAG GCAAAGAAGT GGAGAACGTG 
TTCACTATCC GGACGCCAGA GGATGCCAAT CGCGTGGTGA GGCTGGCCCG AGGCCGCAAC 
GTGGTCGTCG TGGGAGCCGG CTTCCTGGGG ATGGAGGTGG CCGCTTACCT GACGGAGAAG 
GCCCACTCTG TGTCTGTGGT GGAGCTGGAG GAGACGCCCT TCAGGAGGTT CCTGGGGGAG 
CGCGTGGGTC GTGCCCTCAT GAAGATGTTT GAGAACAACC GGGTGAAGTT CTACATGCAG 
ACGGAGGTGT CTGAGCTGCG GGGCCAGGAG GGAAAGCTGA AGGAGGTTGT GCTGAAGAGC 
AGCAAGGTCG TGCGGGCTGA CGTCTGCGTG GTGGGCATTG GTGCAGTGCC CGCCACAGGC 
TTCCTGAGGC AAAGCGGCAT CGGTTTGGAT TCCCGAGGCT TCATCCCTGT CAACAAGATG 
ATGCAGACCA ATGTCCCAGG CGTGTTTGCA GCTGGCGATG CTGTCACCTT CCCCCTTGCC 
TGGAGGAACA ACCGCAAAGT GAACATTCCA CATTGGCAGA TGGCTCATGC TCAGGGGCGC 
GTGGCAGCCC AGAACATGTT GGCGCAGGAG GCGGAGATGA GCACTGTGCC CTACCTCTGG 
ACCGCCATGT TTGGCAAGAG CCTGCGCTAC GCGGGCTACG GAGAAGGCTT CGACGACGTC 
ATCATCCAGG GGGATCTGGA GGAGCTGAAG TTTGTGGCTT TTTACACTAA AGGCGACGAG 
GTGATCGCCG TGGCCAGCAT GAACTACGAT CCCATTGTGT CCAAGGTCGC TGAGGTGCTG 
GCCTCAGGCC GTGCCATCCG GAAGCGGGAG GTGGAGACTG GCGACATGTC CTGGCTTACG 
GGGAAAGGAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139760.1
CDS	190..2004
Translation	MGGCFSKPKPGAALPTVELKIEVVLPEKERGKEELSASGKGSPRAYQGNGTARHFHTEERLSTPHPYPSPQDCVEAAVCHVKDLENGQMREVELGWGKVLLVKDNGEFHALGHKCPHYGAPLVKGVLSRGRVRCPWHGACFNISTGDLEDFPGLDSLHKFQVKIEKEKVYVRASKQALQLQRRTKVMAKCISPSAGYSSSTNVLIVGAGAAGLVCAETLRQEGFSDRIVLCTLDRHLPYDRPKLSKSLDTQPEQLALRPKEFFRAYGIEVLTEAQVVTVDVRTKKVVFKDGFKLEYSKLLLAPGSSPKTLSCKGKEVENVFTIRTPEDANRVVRLARGRNVVVVGAGFLGMEVAAYLTEKAHSVSVVELEETPFRRFLGERVGRALMKMFENNRVKFYMQTEVSELRGQEGKLKEVVLKSSKVVRADVCVVGIGAVPATGFLRQSGIGLDSRGFIPVNKMMQTNVPGVFAAGDAVTFPLAWRNNRKVNIPHWQMAHAQGRVAAQNMLAQEAEMSTVPYLWTAMFGKSLRYAGYGEGFDDVIIQGDLEELKFVAFYTKGDEVIAVASMNYDPIVSKVAEVLASGRAIRKREVETGDMSWLTGKGS

Target ORF information:

RefSeq Version	NM_001146288.2
Organism	Homo sapiens(human)
Definition	Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146288.2

ORF Insert Sequence:

ATGGGCGGCT GCTTCTCCAA ACCCAAACCA GGCGCTGCCT TGCCCACAGT GGAGCTCAAG 
ATCGAGGTGG TGCTGCCTGA GAAGGAGCGA GGCAAGGAGG AGCTGTCGGC CAGTGGGAAG 
GGCAGCCCCC GGGCCTACCA GGGCAATGGC ACGGCCCGCC ACTTCCACAC GGAGGAGCGC 
CTGTCCACCC CTCACCCCTA CCCCAGCCCT CAGGATTGCG TGGAGGCTGC TGTCTGCCAC 
GTCAAGGACC TCGAGAATGG CCAGATGCGG GAAGTGGAGC TGGGCTGGGG GAAGGTGTTG 
CTGGTGAAGG ACAATGGGGA GTTCCACGCC CTGGGCCATA AGTGTCCGCA CTACGGCGCA 
CCCCTGGTGA AAGGCGTTCT GTCCCGTGGT CGGGTGCGCT GCCCCTGGCA CGGCGCCTGC 
TTCAACATCA GCACTGGGGA CCTGGAGGAC TTCCCTGGCC TGGACAGTCT ACACAAGTTC 
CAGGTGAAGA TTGAGAAGGA GAAGGTGTAC GTCCGGGCCA GCAAGCAGGC CCTACAGCTG 
CAGCGAAGGA CCAAGGTGAT GGCCAAGTGT ATCTCTCCAA GTGCTGGGTA CAGCAGTAGC 
ACCAATGTGC TCATTGTGGG TGCAGGTGCA GCTGGCCTGG TGTGTGCAGA GACACTGCGG 
CAGGAGGGCT TCTCCGACCG GATCGTCCTG TGCACGCTAG ACCGGCACCT TCCCTACGAC 
CGTCCCAAGC TCAGCAAGTC CCTGGACACA CAGCCTGAGC AGCTGGCCCT GAGGCCCAAG 
GAGTTTTTCC GAGCCTATGG CATCGAGGTG CTCACCGAGG CTCAGGTGGT CACAGTGGAC 
GTGAGAACTA AGAAGGTCGT GTTCAAGGAT GGCTTCAAGC TGGAGTACAG CAAGCTGCTG 
CTGGCACCAG GGAGCAGCCC CAAGACTCTG AGCTGCAAAG GCAAAGAAGT GGAGAACGTG 
TTCACTATCC GGACGCCAGA GGATGCCAAT CGCGTGGTGA GGCTGGCCCG AGGCCGCAAC 
GTGGTCGTCG TGGGAGCCGG CTTCCTGGGG ATGGAGGTGG CCGCTTACCT GACGGAGAAG 
GCCCACTCTG TGTCTGTGGT GGAGCTGGAG GAGACGCCCT TCAGGAGGTT CCTGGGGGAG 
CGCGTGGGTC GTGCCCTCAT GAAGATGTTT GAGAACAACC GGGTGAAGTT CTACATGCAG 
ACGGAGGTGT CTGAGCTGCG GGGCCAGGAG GGAAAGCTGA AGGAGGTTGT GCTGAAGAGC 
AGCAAGGTCG TGCGGGCTGA CGTCTGCGTG GTGGGCATTG GTGCAGTGCC CGCCACAGGC 
TTCCTGAGGC AAAGCGGCAT CGGTTTGGAT TCCCGAGGCT TCATCCCTGT CAACAAGATG 
ATGCAGACCA ATGTCCCAGG CGTGTTTGCA GCTGGCGATG CTGTCACCTT CCCCCTTGCC 
TGGAGGAACA ACCGCAAAGT GAACATTCCA CATTGGCAGA TGGCTCATGC TCAGGGGCGC 
GTGGCAGCCC AGAACATGTT GGCGCAGGAG GCGGAGATGA GCACTGTGCC CTACCTCTGG 
ACCGCCATGT TTGGCAAGAG CCTGCGCTAC GCGGGCTACG GAGAAGGCTT CGACGACGTC 
ATCATCCAGG GGGATCTGGA GGAGCTGAAG TTTGTGGCTT TTTACACTAA AGGCGACGAG 
GTGATCGCCG TGGCCAGCAT GAACTACGAT CCCATTGTGT CCAAGGTCGC TGAGGTGCTG 
GCCTCAGGCC GTGCCATCCG GAAGCGGGAG GTGGAGACTG GCGACATGTC CTGGCTTACG 
GGGAAAGGAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AIFM3 ( NM_001146288.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139760.1 Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 3, mRNA.

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CloneID	OHu31437
Clone ID Related Accession (Same CDS sequence)	NM_001146288.2 , NM_001146288.1
Accession Version	NM_001146288.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-20
Organism	Homo sapiens(human)
Product	apoptosis-inducing factor 3 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB172173.1, AK094844.1, AK295581.1 and BQ028401.1. Transcript Variant: This variant (3) differs in the 5' UTR and has multiple coding region differences compared to variant 1. The encoded isoform (3) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK094844.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 15764604; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGCT GCTTCTCCAA ACCCAAACCA GGCGCTGCCT TGCCCACAGT GGAGCTCAAG 
ATCGAGGTGG TGCTGCCTGA GAAGGAGCGA GGCAAGGAGG AGCTGTCGGC CAGTGGGAAG 
GGCAGCCCCC GGGCCTACCA GGGCAATGGC ACGGCCCGCC ACTTCCACAC GGAGGAGCGC 
CTGTCCACCC CTCACCCCTA CCCCAGCCCT CAGGATTGCG TGGAGGCTGC TGTCTGCCAC 
GTCAAGGACC TCGAGAATGG CCAGATGCGG GAAGTGGAGC TGGGCTGGGG GAAGGTGTTG 
CTGGTGAAGG ACAATGGGGA GTTCCACGCC CTGGGCCATA AGTGTCCGCA CTACGGCGCA 
CCCCTGGTGA AAGGCGTTCT GTCCCGTGGT CGGGTGCGCT GCCCCTGGCA CGGCGCCTGC 
TTCAACATCA GCACTGGGGA CCTGGAGGAC TTCCCTGGCC TGGACAGTCT ACACAAGTTC 
CAGGTGAAGA TTGAGAAGGA GAAGGTGTAC GTCCGGGCCA GCAAGCAGGC CCTACAGCTG 
CAGCGAAGGA CCAAGGTGAT GGCCAAGTGT ATCTCTCCAA GTGCTGGGTA CAGCAGTAGC 
ACCAATGTGC TCATTGTGGG TGCAGGTGCA GCTGGCCTGG TGTGTGCAGA GACACTGCGG 
CAGGAGGGCT TCTCCGACCG GATCGTCCTG TGCACGCTAG ACCGGCACCT TCCCTACGAC 
CGTCCCAAGC TCAGCAAGTC CCTGGACACA CAGCCTGAGC AGCTGGCCCT GAGGCCCAAG 
GAGTTTTTCC GAGCCTATGG CATCGAGGTG CTCACCGAGG CTCAGGTGGT CACAGTGGAC 
GTGAGAACTA AGAAGGTCGT GTTCAAGGAT GGCTTCAAGC TGGAGTACAG CAAGCTGCTG 
CTGGCACCAG GGAGCAGCCC CAAGACTCTG AGCTGCAAAG GCAAAGAAGT GGAGAACGTG 
TTCACTATCC GGACGCCAGA GGATGCCAAT CGCGTGGTGA GGCTGGCCCG AGGCCGCAAC 
GTGGTCGTCG TGGGAGCCGG CTTCCTGGGG ATGGAGGTGG CCGCTTACCT GACGGAGAAG 
GCCCACTCTG TGTCTGTGGT GGAGCTGGAG GAGACGCCCT TCAGGAGGTT CCTGGGGGAG 
CGCGTGGGTC GTGCCCTCAT GAAGATGTTT GAGAACAACC GGGTGAAGTT CTACATGCAG 
ACGGAGGTGT CTGAGCTGCG GGGCCAGGAG GGAAAGCTGA AGGAGGTTGT GCTGAAGAGC 
AGCAAGGTCG TGCGGGCTGA CGTCTGCGTG GTGGGCATTG GTGCAGTGCC CGCCACAGGC 
TTCCTGAGGC AAAGCGGCAT CGGTTTGGAT TCCCGAGGCT TCATCCCTGT CAACAAGATG 
ATGCAGACCA ATGTCCCAGG CGTGTTTGCA GCTGGCGATG CTGTCACCTT CCCCCTTGCC 
TGGAGGAACA ACCGCAAAGT GAACATTCCA CATTGGCAGA TGGCTCATGC TCAGGGGCGC 
GTGGCAGCCC AGAACATGTT GGCGCAGGAG GCGGAGATGA GCACTGTGCC CTACCTCTGG 
ACCGCCATGT TTGGCAAGAG CCTGCGCTAC GCGGGCTACG GAGAAGGCTT CGACGACGTC 
ATCATCCAGG GGGATCTGGA GGAGCTGAAG TTTGTGGCTT TTTACACTAA AGGCGACGAG 
GTGATCGCCG TGGCCAGCAT GAACTACGAT CCCATTGTGT CCAAGGTCGC TGAGGTGCTG 
GCCTCAGGCC GTGCCATCCG GAAGCGGGAG GTGGAGACTG GCGACATGTC CTGGCTTACG 
GGGAAAGGAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139760.1
CDS	286..2100
Translation	MGGCFSKPKPGAALPTVELKIEVVLPEKERGKEELSASGKGSPRAYQGNGTARHFHTEERLSTPHPYPSPQDCVEAAVCHVKDLENGQMREVELGWGKVLLVKDNGEFHALGHKCPHYGAPLVKGVLSRGRVRCPWHGACFNISTGDLEDFPGLDSLHKFQVKIEKEKVYVRASKQALQLQRRTKVMAKCISPSAGYSSSTNVLIVGAGAAGLVCAETLRQEGFSDRIVLCTLDRHLPYDRPKLSKSLDTQPEQLALRPKEFFRAYGIEVLTEAQVVTVDVRTKKVVFKDGFKLEYSKLLLAPGSSPKTLSCKGKEVENVFTIRTPEDANRVVRLARGRNVVVVGAGFLGMEVAAYLTEKAHSVSVVELEETPFRRFLGERVGRALMKMFENNRVKFYMQTEVSELRGQEGKLKEVVLKSSKVVRADVCVVGIGAVPATGFLRQSGIGLDSRGFIPVNKMMQTNVPGVFAAGDAVTFPLAWRNNRKVNIPHWQMAHAQGRVAAQNMLAQEAEMSTVPYLWTAMFGKSLRYAGYGEGFDDVIIQGDLEELKFVAFYTKGDEVIAVASMNYDPIVSKVAEVLASGRAIRKREVETGDMSWLTGKGS

Target ORF information:

RefSeq Version	NM_001146288.1
Organism	Homo sapiens(human)
Definition	Homo sapiens apoptosis inducing factor mitochondria associated 3 (AIFM3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146288.1

ORF Insert Sequence:

ATGGGCGGCT GCTTCTCCAA ACCCAAACCA GGCGCTGCCT TGCCCACAGT GGAGCTCAAG 
ATCGAGGTGG TGCTGCCTGA GAAGGAGCGA GGCAAGGAGG AGCTGTCGGC CAGTGGGAAG 
GGCAGCCCCC GGGCCTACCA GGGCAATGGC ACGGCCCGCC ACTTCCACAC GGAGGAGCGC 
CTGTCCACCC CTCACCCCTA CCCCAGCCCT CAGGATTGCG TGGAGGCTGC TGTCTGCCAC 
GTCAAGGACC TCGAGAATGG CCAGATGCGG GAAGTGGAGC TGGGCTGGGG GAAGGTGTTG 
CTGGTGAAGG ACAATGGGGA GTTCCACGCC CTGGGCCATA AGTGTCCGCA CTACGGCGCA 
CCCCTGGTGA AAGGCGTTCT GTCCCGTGGT CGGGTGCGCT GCCCCTGGCA CGGCGCCTGC 
TTCAACATCA GCACTGGGGA CCTGGAGGAC TTCCCTGGCC TGGACAGTCT ACACAAGTTC 
CAGGTGAAGA TTGAGAAGGA GAAGGTGTAC GTCCGGGCCA GCAAGCAGGC CCTACAGCTG 
CAGCGAAGGA CCAAGGTGAT GGCCAAGTGT ATCTCTCCAA GTGCTGGGTA CAGCAGTAGC 
ACCAATGTGC TCATTGTGGG TGCAGGTGCA GCTGGCCTGG TGTGTGCAGA GACACTGCGG 
CAGGAGGGCT TCTCCGACCG GATCGTCCTG TGCACGCTAG ACCGGCACCT TCCCTACGAC 
CGTCCCAAGC TCAGCAAGTC CCTGGACACA CAGCCTGAGC AGCTGGCCCT GAGGCCCAAG 
GAGTTTTTCC GAGCCTATGG CATCGAGGTG CTCACCGAGG CTCAGGTGGT CACAGTGGAC 
GTGAGAACTA AGAAGGTCGT GTTCAAGGAT GGCTTCAAGC TGGAGTACAG CAAGCTGCTG 
CTGGCACCAG GGAGCAGCCC CAAGACTCTG AGCTGCAAAG GCAAAGAAGT GGAGAACGTG 
TTCACTATCC GGACGCCAGA GGATGCCAAT CGCGTGGTGA GGCTGGCCCG AGGCCGCAAC 
GTGGTCGTCG TGGGAGCCGG CTTCCTGGGG ATGGAGGTGG CCGCTTACCT GACGGAGAAG 
GCCCACTCTG TGTCTGTGGT GGAGCTGGAG GAGACGCCCT TCAGGAGGTT CCTGGGGGAG 
CGCGTGGGTC GTGCCCTCAT GAAGATGTTT GAGAACAACC GGGTGAAGTT CTACATGCAG 
ACGGAGGTGT CTGAGCTGCG GGGCCAGGAG GGAAAGCTGA AGGAGGTTGT GCTGAAGAGC 
AGCAAGGTCG TGCGGGCTGA CGTCTGCGTG GTGGGCATTG GTGCAGTGCC CGCCACAGGC 
TTCCTGAGGC AAAGCGGCAT CGGTTTGGAT TCCCGAGGCT TCATCCCTGT CAACAAGATG 
ATGCAGACCA ATGTCCCAGG CGTGTTTGCA GCTGGCGATG CTGTCACCTT CCCCCTTGCC 
TGGAGGAACA ACCGCAAAGT GAACATTCCA CATTGGCAGA TGGCTCATGC TCAGGGGCGC 
GTGGCAGCCC AGAACATGTT GGCGCAGGAG GCGGAGATGA GCACTGTGCC CTACCTCTGG 
ACCGCCATGT TTGGCAAGAG CCTGCGCTAC GCGGGCTACG GAGAAGGCTT CGACGACGTC 
ATCATCCAGG GGGATCTGGA GGAGCTGAAG TTTGTGGCTT TTTACACTAA AGGCGACGAG 
GTGATCGCCG TGGCCAGCAT GAACTACGAT CCCATTGTGT CCAAGGTCGC TGAGGTGCTG 
GCCTCAGGCC GTGCCATCCG GAAGCGGGAG GTGGAGACTG GCGACATGTC CTGGCTTACG 
GGGAAAGGAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High expression of apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) in human cholangiocarcinoma.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(10)13659-13667(2016 Oct)
Chua-On D,Proungvitaya T,Techasen A,Limpaiboon T,Roytrakul S,Wongkham S,Wongkham C,Somintara O,Sungkhamanon S,Proungvitaya S

Downregulation of miR-210 expression inhibits proliferation, induces apoptosis and enhances radiosensitivity in hypoxic human hepatoma cells in vitro.
Experimental cell research318(8)944-54(2012 May)
Yang W,Sun T,Cao J,Liu F,Tian Y,Zhu W

[Expression of AIF and CGRP markers in pineal gland and thymus during aging].
Advances in gerontology = Uspekhi gerontologii24(4)601-5(2011)
Lin'kova NS,Katanugina AS,Khavinson VKh

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

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AIFM3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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