CTLA4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CTLA4
Entrez Gene ID	1493
Full Name	cytotoxic T-lymphocyte associated protein 4
Synonyms	ALPS5,CD,CD152,CELIAC3,CTLA-4,GRD4,GSE,IDDM12
General protein information	Preferred Names cytotoxic T-lymphocyte associated protein 4 Names cytotoxic T-lymphocyte protein 4 CD152 isoform celiac disease 3 cytotoxic T lymphocyte associated antigen 4 short spliced form cytotoxic T-lymphocyte-associated serine esterase-4 insulin-dependent diabetes mellitus 12 ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q33.2
Summary	This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008].
Disorder MIM:	123890
Disorder Html:	{Hashimoto thyroiditis}, 140300 (3); {Systemic lupus erythematosus, susceptibility to}, 152700 (3); {Diabetes mellitus, insulin-dependent, 12}, 601388 (3); {Celiac disease, susceptibility to, 3}, 609755 (3); Autoimmune lymphoproliferative syndrome, type V, 616100 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037631.2	NP_001032720.1	cytotoxic T-lymphocyte protein 4 isoform CTLA-4delTM precursor
NM_001037631.3	NP_001032720.1	cytotoxic T-lymphocyte protein 4 isoform CTLA-4delTM
NM_005214.4	NP_005205.2	cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor
NM_005214.5	NP_005205.2	cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ctla4	NP_113862.1
Pan troglodytes (chimpanzee)	CTLA4	XP_526000.1
Mus musculus (house mouse)	Ctla4	NP_033973.2
Xenopus tropicalis (tropical clawed frog)	ctla4	XP_002936576.1
Bos taurus (cattle)	CTLA4	NP_776722.1
Homo sapiens (human)	CTLA4	NP_005205.2
	CTLA4	NP_001038204.1
Canis lupus familiaris (dog)	CTLA4	NP_001003106.1
Gallus gallus (chicken)	CTLA4	NP_001035180.1

Gene Ontology
Bibliography

Related articles in PubMed

CD25-expressing Th17 cells mediate CD8
Lang C, Wang J, Chen L
Experimental cell research360(2)384-389(2017 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CTLA-4g was an independent Graves' disease risk factor. Sporadic Graves' disease was related to presence of CTLA-4c allele variant. Familial background of the disease was exclusively associated with CTLA-4g genotype.
Title: CD28/CTLA-4/ICOS haplotypes confers susceptibility to Graves' disease and modulates clinical phenotype of disease.

Reduced CTLA4 secretion and specific CTLA4 variants may contribute to the pathogenesis of idiopathic recurrent miscarriages.
Title: Association of functional genetic variants of CTLA4 with reduced serum CTLA4 protein levels and increased risk of idiopathic recurrent miscarriages.

No significant association was found between +49A/G (rs231775), -318C/T (rs5742909), and +6230A/G (rs3087243) CTLA-4 gene polymorphisms and lymphoid malignancies.
Title: Lack of association between cytotoxic T-lymphocyte antigen-4 gene polymorphisms and lymphoid malignancy risk: evidence from a meta-analysis.

Of several immunotherapies being investigated, antibodies that target the programmed cell death protein-1 (nivolumab and pembrolizumab) and cytotoxic T-lymphocyte antigen-4 (ipilimumab) immune checkpoint pathways are among the most promising for patients with Small cell lung cancer (SCLC), and are the focus of this review.
Title: The Future of Immunotherapy in the Treatment of Small Cell Lung Cancer.

findings indicate that CTLA-4 (+49 A/G) and (-318 C/T) genotypes could be considered as genetic risk factors associated with susceptibility or protection for type 2 diabetes.
Title: Cytotoxic T Lymphocyte Antigen-4 Gene Variants in Type 2 Diabetic Patients with or without Neuropathy.

The following CTLA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CTLA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22372	NM_001037631.3 Latest version!	Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu23246	NM_005214.5 Latest version!	Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu22372	NM_001037631.2	Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu23246	NM_005214.4	Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CTLA4 ( NM_001037631.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001032720.1 Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22372
Clone ID Related Accession (Same CDS sequence)	NM_001037631.2 , NM_001037631.3
Accession Version	NM_001037631.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	cytotoxic T-lymphocyte protein 4 isoform CTLA-4delTM precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF414120.1 and AC010138.6. On Jul 2, 2011 this sequence version replaced NM_001037631.1. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform CTLA-4delTM (also known as sCTLA4) is soluble and lacks the transmembrane domain, compared to isoform a. The exon skip represented in this variant is is based on human U90273.1, and is consistent with mouse U90270.1 and the data published in PMID:10831323 and PMID:10556814. CCDS Note: This CCDS representation is based on U90273.1 and on full-length RT-PCR evidence from PMIDs 10831323 and 10556814. This variant also has homology support from cow AF539438.1, mouse U90270.1 and rat U90271.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG 
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT 
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG 
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT 
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG 
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC 
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGCTA AAGAAAAGAA GCCCTCTTAC 
AACAGGGGTC TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032720.1
CDS	156..680
Translation	MACLGFQRHKAQLNLATRTWPCTLLFFLLFIPVFCKAMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIAKEKKPSYNRGLCENAPNRARM

Target ORF information:

RefSeq Version	NM_001037631.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037631.2

ORF Insert Sequence:

ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG 
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT 
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG 
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT 
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG 
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC 
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGCTA AAGAAAAGAA GCCCTCTTAC 
AACAGGGGTC TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CTLA4 ( NM_001037631.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001032720.1 Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22372
Clone ID Related Accession (Same CDS sequence)	NM_001037631.2 , NM_001037631.3
Accession Version	NM_001037631.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	cytotoxic T-lymphocyte protein 4 isoform CTLA-4delTM
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010138.6, AF414120.1 and AI733018.1. On May 29, 2018 this sequence version replaced NM_001037631.2. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform CTLA-4delTM (also known as sCTLA4) is soluble and lacks the transmembrane domain, compared to isoform a. The exon skip represented in this variant is is based on human U90273.1, and is consistent with mouse U90270.1 and the data published in PMID:10831323 and PMID:10556814. CCDS Note: This CCDS representation is based on U90273.1 and on full-length RT-PCR evidence from PMIDs 10831323 and 10556814. This variant also has homology support from cow AF539438.1, mouse U90270.1 and rat U90271.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG 
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT 
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG 
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT 
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG 
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC 
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGCTA AAGAAAAGAA GCCCTCTTAC 
AACAGGGGTC TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032720.1
CDS	173..697
Translation	MACLGFQRHKAQLNLATRTWPCTLLFFLLFIPVFCKAMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIAKEKKPSYNRGLCENAPNRARM

Target ORF information:

RefSeq Version	NM_001037631.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037631.3

ORF Insert Sequence:

ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG 
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT 
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG 
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT 
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG 
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC 
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGCTA AAGAAAAGAA GCCCTCTTAC 
AACAGGGGTC TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CTLA4 ( NM_005214.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005205.2 Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23246
Clone ID Related Accession (Same CDS sequence)	NM_005214.4 , NM_005214.5
Accession Version	NM_005214.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF414120.1 and AC010138.6. This sequence is a reference standard in the RefSeqGene project. On Jul 2, 2011 this sequence version replaced NM_005214.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer membrane-bound isoform CTLA4-TM. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF414120.1, BC069566.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG 
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT 
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG 
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT 
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG 
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC 
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGATC CAGAACCGTG CCCAGATTCT 
GACTTCCTCC TCTGGATCCT TGCAGCAGTT AGTTCGGGGT TGTTTTTTTA TAGCTTTCTC 
CTCACAGCTG TTTCTTTGAG CAAAATGCTA AAGAAAAGAA GCCCTCTTAC AACAGGGGTC 
TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGAAAAGC AATTTCAGCC TTATTTTATT 
CCCATCAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005205.2
CDS	156..827
Translation	MACLGFQRHKAQLNLATRTWPCTLLFFLLFIPVFCKAMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSDFLLWILAAVSSGLFFYSFLLTAVSLSKMLKKRSPLTTGVYVKMPPTEPECEKQFQPYFIPIN

Target ORF information:

RefSeq Version	NM_005214.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005214.4

ORF Insert Sequence:

ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG 
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT 
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG 
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT 
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG 
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC 
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGATC CAGAACCGTG CCCAGATTCT 
GACTTCCTCC TCTGGATCCT TGCAGCAGTT AGTTCGGGGT TGTTTTTTTA TAGCTTTCTC 
CTCACAGCTG TTTCTTTGAG CAAAATGCTA AAGAAAAGAA GCCCTCTTAC AACAGGGGTC 
TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGAAAAGC AATTTCAGCC TTATTTTATT 
CCCATCAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CTLA4 ( NM_005214.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005205.2 Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23246
Clone ID Related Accession (Same CDS sequence)	NM_005214.4 , NM_005214.5
Accession Version	NM_005214.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010138.6, AF414120.1 and AI733018.1. This sequence is a reference standard in the RefSeqGene project. On May 29, 2018 this sequence version replaced NM_005214.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer membrane-bound isoform CTLA4-TM. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF414120.1, BC069566.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000648405.2/ ENSP00000497102.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG 
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT 
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG 
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT 
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG 
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC 
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGATC CAGAACCGTG CCCAGATTCT 
GACTTCCTCC TCTGGATCCT TGCAGCAGTT AGTTCGGGGT TGTTTTTTTA TAGCTTTCTC 
CTCACAGCTG TTTCTTTGAG CAAAATGCTA AAGAAAAGAA GCCCTCTTAC AACAGGGGTC 
TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGAAAAGC AATTTCAGCC TTATTTTATT 
CCCATCAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005205.2
CDS	173..844
Translation	MACLGFQRHKAQLNLATRTWPCTLLFFLLFIPVFCKAMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSDFLLWILAAVSSGLFFYSFLLTAVSLSKMLKKRSPLTTGVYVKMPPTEPECEKQFQPYFIPIN

Target ORF information:

RefSeq Version	NM_005214.5
Organism	Homo sapiens(human)
Definition	Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005214.5

ORF Insert Sequence:

ATGGCTTGCC TTGGATTTCA GCGGCACAAG GCTCAGCTGA ACCTGGCTAC CAGGACCTGG 
CCCTGCACTC TCCTGTTTTT TCTTCTCTTC ATCCCTGTCT TCTGCAAAGC AATGCACGTG 
GCCCAGCCTG CTGTGGTACT GGCCAGCAGC CGAGGCATCG CCAGCTTTGT GTGTGAGTAT 
GCATCTCCAG GCAAAGCCAC TGAGGTCCGG GTGACAGTGC TTCGGCAGGC TGACAGCCAG 
GTGACTGAAG TCTGTGCGGC AACCTACATG ATGGGGAATG AGTTGACCTT CCTAGATGAT 
TCCATCTGCA CGGGCACCTC CAGTGGAAAT CAAGTGAACC TCACTATCCA AGGACTGAGG 
GCCATGGACA CGGGACTCTA CATCTGCAAG GTGGAGCTCA TGTACCCACC GCCATACTAC 
CTGGGCATAG GCAACGGAAC CCAGATTTAT GTAATTGATC CAGAACCGTG CCCAGATTCT 
GACTTCCTCC TCTGGATCCT TGCAGCAGTT AGTTCGGGGT TGTTTTTTTA TAGCTTTCTC 
CTCACAGCTG TTTCTTTGAG CAAAATGCTA AAGAAAAGAA GCCCTCTTAC AACAGGGGTC 
TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGAAAAGC AATTTCAGCC TTATTTTATT 
CCCATCAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CD25-expressing Th17 cells mediate CD8
Experimental cell research360(2)384-389(2017 11)
Lang C,Wang J,Chen L

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CTLA4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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