gRNAs
The following ANKRD35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKRD35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu11215 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001280799.1 | |
| Accession Version | NM_001280799.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-06-29 | |
| Organism | Homo sapiens(human) | |
| Product | ankyrin repeat domain-containing protein 35 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA399526.1, AK294308.1, AC243547.1 and HY327286.1. Transcript Variant: This variant (2) lacks an in-frame segment in the 5' coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGAAGCGTA TCTTCTCCTG CTCCAGCACA CAGTGTGTTA AGGTTCTGCT TCAGCATGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001267728.1 |
| CDS | 109..2844 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001280799.1 |
1 | ATGAAGCGTA TCTTCTCCTG CTCCAGCACA CAGTGTGTTA AGGTTCTGCT TCAGCATGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu12188 | |
| Clone ID Related Accession (Same CDS sequence) | NM_144698.4 , NM_144698.5 | |
| Accession Version | NM_144698.4 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-01 | |
| Organism | Homo sapiens(human) | |
| Product | ankyrin repeat domain-containing protein 35 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA399526.1, BC101618.1, AK294308.1, AC243547.1 and HY327286.1. On Jul 23, 2013 this sequence version replaced NM_144698.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK091120.1, SRR1803613.134397.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGAAGCGTA TCTTCTCCTG CTCCAGCACA CAGGTGGCGG TGGAGAGATG GAACCGCCAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_653299.4 |
| CDS | 109..3114 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_144698.4 |
1 | ATGAAGCGTA TCTTCTCCTG CTCCAGCACA CAGGTGGCGG TGGAGAGATG GAACCGCCAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu12188 | |
| Clone ID Related Accession (Same CDS sequence) | NM_144698.4 , NM_144698.5 | |
| Accession Version | NM_144698.5 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-30 | |
| Organism | Homo sapiens(human) | |
| Product | ankyrin repeat domain-containing protein 35 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA399526.1, BC101618.1, AK294308.1, AC243547.1 and HY327286.1. On Nov 22, 2018 this sequence version replaced NM_144698.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.134397.1, AK091120.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000355594.9/ ENSP00000347802.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. | |
1 | ATGAAGCGTA TCTTCTCCTG CTCCAGCACA CAGGTGGCGG TGGAGAGATG GAACCGCCAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_653299.4 |
| CDS | 109..3114 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_144698.5 |
1 | ATGAAGCGTA TCTTCTCCTG CTCCAGCACA CAGGTGGCGG TGGAGAGATG GAACCGCCAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Expressed sequence tag analysis of human retina for the NEIBank Project: retbindin, an abundant, novel retinal cDNA and alternative splicing of other retina-preferred gene transcripts. |
