CSTB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CSTB
Entrez Gene ID	1476
Full Name	cystatin B
Synonyms	CPI-B,CST6,EPM1,EPM1A,PME,STFB,ULD
General protein information	Preferred Names cystatin B Names cystatin-B cystatin B (stefin B) liver thiol proteinase inhibitor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q22.3
Summary	The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies. [provided by RefSeq, Jul 2016].
Disorder MIM:	601145
Disorder Html:	Epilepsy, progressive myoclonic 1A (Unverricht and Lundborg), 254800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000100.4	NP_000091.1	cystatin-B
NM_000100.3	NP_000091.1	cystatin-B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cstb	NP_031819.1
Gallus gallus (chicken)	CSTB	NP_001185577.1
Danio rerio (zebrafish)	zgc:56530	NP_001096599.1
	CSTB	XP_001104524.1
Bos taurus (cattle)	CSTB	NP_001032538.1
Danio rerio (zebrafish)	zgc:153129	NP_001070742.1
Danio rerio (zebrafish)	si:ch211-161h7.6	XP_698401.1
Rattus norvegicus (Norway rat)	Cstb	NP_036970.1
Homo sapiens (human)	CSTB	NP_000091.1
Pan troglodytes (chimpanzee)	CSTB	NP_001009095.1

Gene Ontology
Bibliography

Related articles in PubMed

Tumor Necrosis Factor-? Induced Apoptosis in U937 Cells Promotes Cathepsin D-Independent Stefin B Degradation.
Bidovec K, Bo?i? J, Dolenc I, Turk B, Turk V, Stoka V
Journal of cellular biochemistry118(12)4813-4820(2017 Dec)

Expression and epigenetic regulation of cystatin B in lung cancer and colorectal cancer.
Ma Y, Chen Y, Petersen I
Pathology, research and practice213(12)1568-1574(2017 Dec)

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V, Cutillas PR, Balkwill FR, Hynes RO
Journal of proteome research16(8)3083-3091(2017 Aug)

Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis.
Uni? A, Derek L, Duvnjak M, Patrlj L, Raki? M, Kujund?i? M, Renji? V, ?tokovi? N, Dinjar P, Jukic A, Grgurevi? I
Annals of clinical biochemistry4563217726808(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

apoptosis is accompanied by degradation of the cysteine cathepsin inhibitor stefin B (StfB). CatD did not exhibit a crucial role in this step. However, this degradation was partially prevented through pre-incubation with the antioxidant N-acetyl cysteine
Title: Tumor Necrosis Factor-? Induced Apoptosis in U937 Cells Promotes Cathepsin D-Independent Stefin B Degradation.

Homozygous for a c.218dupT (p.His75Serfs*2) mutation in exon 3 of CSTB causes neurodegeneration, progressive cerebral volume loss and diffuse hypomyelination.
Title: Severe neurodegeneration, progressive cerebral volume loss and diffuse hypomyelination associated with a homozygous frameshift mutation in CSTB.

CSTB downregulation may promote the development of gastric cancer.
Title: CSTB Downregulation Promotes Cell Proliferation and Migration and Suppresses Apoptosis in Gastric Cancer SGC-7901 Cell Line.

It was shown that decreased expression of cystatin B enhances cathepsin activity in Niemann-Pick C cerebellar degeneration patient fibroblasts.
Title: Genetic and pharmacological evidence implicates cathepsins in Niemann-Pick C cerebellar degeneration.

CSTB null mutation associated with microcephaly, early developmental delay, and severe dyskinesia.
Title: CSTB null mutation associated with microcephaly, early developmental delay, and severe dyskinesia.

The following CSTB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CSTB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23045	NM_000100.4 Latest version!	Homo sapiens cystatin B (CSTB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu23045	NM_000100.3	Homo sapiens cystatin B (CSTB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CSTB ( NM_000100.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000091.1 Homo sapiens cystatin B (CSTB), mRNA.

Price & Availability↑

CloneID	OHu23045
Clone ID Related Accession (Same CDS sequence)	NM_000100.4 , NM_000100.3
Accession Version	NM_000100.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	cystatin-B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC003370.1. On Nov 21, 2019 this sequence version replaced NM_000100.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BE794956.1, BQ691925.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGTGCG GGGCGCCCTC CGCCACGCAG CCGGCCACCG CCGAGACCCA GCACATCGCC 
GACCAGGTGA GGTCCCAGCT TGAAGAGAAA GAAAACAAGA AGTTCCCTGT GTTTAAGGCC 
GTGTCATTCA AGAGCCAGGT GGTCGCGGGG ACAAACTACT TCATCAAGGT GCACGTCGGC 
GACGAGGACT TCGTACACCT GCGAGTGTTC CAATCTCTCC CTCATGAAAA CAAGCCCTTG 
ACCTTATCTA ACTACCAGAC CAACAAAGCC AAGCATGATG AGCTGACCTA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000091.1
CDS	40..336
Translation	MMCGAPSATQPATAETQHIADQVRSQLEEKENKKFPVFKAVSFKSQVVAGTNYFIKVHVGDEDFVHLRVFQSLPHENKPLTLSNYQTNKAKHDELTYF

Target ORF information:

RefSeq Version	NM_000100.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cystatin B (CSTB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000100.4

ORF Insert Sequence:

ATGATGTGCG GGGCGCCCTC CGCCACGCAG CCGGCCACCG CCGAGACCCA GCACATCGCC 
GACCAGGTGA GGTCCCAGCT TGAAGAGAAA GAAAACAAGA AGTTCCCTGT GTTTAAGGCC 
GTGTCATTCA AGAGCCAGGT GGTCGCGGGG ACAAACTACT TCATCAAGGT GCACGTCGGC 
GACGAGGACT TCGTACACCT GCGAGTGTTC CAATCTCTCC CTCATGAAAA CAAGCCCTTG 
ACCTTATCTA ACTACCAGAC CAACAAAGCC AAGCATGATG AGCTGACCTA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSTB ( NM_000100.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000091.1 Homo sapiens cystatin B (CSTB), mRNA.

Price & Availability↑

CloneID	OHu23045
Clone ID Related Accession (Same CDS sequence)	NM_000100.4 , NM_000100.3
Accession Version	NM_000100.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	cystatin-B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD364415.1, BC003370.1, BX419549.2 and AP001053.1. This sequence is a reference standard in the RefSeqGene project. On Jan 7, 2012 this sequence version replaced NM_000100.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI669925.1, SRR1660809.244603.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGTGCG GGGCGCCCTC CGCCACGCAG CCGGCCACCG CCGAGACCCA GCACATCGCC 
GACCAGGTGA GGTCCCAGCT TGAAGAGAAA GAAAACAAGA AGTTCCCTGT GTTTAAGGCC 
GTGTCATTCA AGAGCCAGGT GGTCGCGGGG ACAAACTACT TCATCAAGGT GCACGTCGGC 
GACGAGGACT TCGTACACCT GCGAGTGTTC CAATCTCTCC CTCATGAAAA CAAGCCCTTG 
ACCTTATCTA ACTACCAGAC CAACAAAGCC AAGCATGATG AGCTGACCTA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000091.1
CDS	107..403
Translation	MMCGAPSATQPATAETQHIADQVRSQLEEKENKKFPVFKAVSFKSQVVAGTNYFIKVHVGDEDFVHLRVFQSLPHENKPLTLSNYQTNKAKHDELTYF

Target ORF information:

RefSeq Version	NM_000100.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cystatin B (CSTB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000100.3

ORF Insert Sequence:

ATGATGTGCG GGGCGCCCTC CGCCACGCAG CCGGCCACCG CCGAGACCCA GCACATCGCC 
GACCAGGTGA GGTCCCAGCT TGAAGAGAAA GAAAACAAGA AGTTCCCTGT GTTTAAGGCC 
GTGTCATTCA AGAGCCAGGT GGTCGCGGGG ACAAACTACT TCATCAAGGT GCACGTCGGC 
GACGAGGACT TCGTACACCT GCGAGTGTTC CAATCTCTCC CTCATGAAAA CAAGCCCTTG 
ACCTTATCTA ACTACCAGAC CAACAAAGCC AAGCATGATG AGCTGACCTA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tumor Necrosis Factor-? Induced Apoptosis in U937 Cells Promotes Cathepsin D-Independent Stefin B Degradation.
Journal of cellular biochemistry118(12)4813-4820(2017 Dec)
Bidovec K,Bo?i? J,Dolenc I,Turk B,Turk V,Stoka V

Expression and epigenetic regulation of cystatin B in lung cancer and colorectal cancer.
Pathology, research and practice213(12)1568-1574(2017 Dec)
Ma Y,Chen Y,Petersen I

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Journal of proteome research16(8)3083-3091(2017 Aug)
Naba A,Pearce OMT,Del Rosario A,Ma D,Ding H,Rajeeve V,Cutillas PR,Balkwill FR,Hynes RO

Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis.
Annals of clinical biochemistry4563217726808(2017 Jan)
Uni? A,Derek L,Duvnjak M,Patrlj L,Raki? M,Kujund?i? M,Renji? V,?tokovi? N,Dinjar P,Jukic A,Grgurevi? I

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CSTB cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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