TMEM199 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM199
Entrez Gene ID	147007
Full Name	transmembrane protein 199
Synonyms	C17orf32,CDG2P,VMA12,VPH2
General protein information	Preferred Names transmembrane protein 199 Names transmembrane protein 199
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q11.2
Summary	The protein encoded by this gene has been observed to localize to the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) and coat protein complex I (COPI) in some human cells. The encoded protein shares some homology with the yeast protein Vma12. Defects in this gene are a cause of congenital disorder of glycosylation, type IIp. [provided by RefSeq, Mar 2016].
Disorder MIM:	616815
Disorder Html:	Congenital disorder of glycosylation, type IIp, 616829 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_152464.2	NP_689677.1	transmembrane protein 199
NM_152464.3	NP_689677.1	transmembrane protein 199

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tmem199	NP_001020163.1
Xenopus tropicalis (tropical clawed frog)	tmem199	NP_001120106.1
Homo sapiens (human)	TMEM199	NP_689677.1
Danio rerio (zebrafish)	tmem199	NP_001099067.1
Mus musculus (house mouse)	Tmem199	NP_954669.2
Pan troglodytes (chimpanzee)	TMEM199	NP_001229551.1
	TMEM199	XP_001106816.1
Canis lupus familiaris (dog)	TMEM199	XP_548283.2
Bos taurus (cattle)	TMEM199	NP_001068980.1
Gallus gallus (chicken)	TMEM199	XP_415812.2

Gene Ontology
Bibliography

Related articles in PubMed

The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels.
Miles AL, Burr SP, Grice GL, Nathan JA
eLife6(2017 03)

TMEM199 Deficiency Is a Disorder of Golgi Homeostasis Characterized by Elevated Aminotransferases, Alkaline Phosphatase, and Cholesterol and Abnormal Glycosylation.
Jansen JC, Timal S, van Scherpenzeel M, Michelakakis H, Vicogne D, Ashikov A, Moraitou M, Hoischen A, Huijben K, Steenbergen G, van den Boogert MA, Porta F, Calvo PL, Mavrikou M, Cenacchi G, van den Bogaart G, Salomon J, Holleboom AG, Rodenburg RJ, Drenth JP, Huynen MA, Wevers RA, Morava E, Foulquier F, Veltman JA, Lefeber DJ
American journal of human genetics98(2)322-30(2016 Feb)

Meta-analysis of genome-wide association studies identifies two loci associated with circulating osteoprotegerin levels.
Kwan JS, Hsu YH, Cheung CL, Dupuis J, Saint-Pierre A, Eriksson J, Handelman SK, Aragaki A, Karasik D, Pramstaller PP, Kooperberg C, Lacroix AZ, Larson MG, Lau KS, Lorentzon M, Pichler I, Sham PC, Taliun D, Vandenput L, Kiel DP, Hicks AA, Jackson RD, Ohlsson C, Benjamin EJ, Kung AW
Human molecular genetics23(24)6684-93(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Vacuolar H+ ATPase (V-ATPase), the key proton pump for endo-lysosomal acidification, and two previously uncharacterised V-ATPase assembly factors, TMEM199 and CCDC115, stabilise HIF1alpha in aerobic conditions.
Title: The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels.

We have identified TMEM199 as a protein involved in Golgi homeostasis and show that TMEM199 deficiency results in a hepatic phenotype with abnormal glycosylation.
Title: TMEM199 Deficiency Is a Disorder of Golgi Homeostasis Characterized by Elevated Aminotransferases, Alkaline Phosphatase, and Cholesterol and Abnormal Glycosylation.

The following TMEM199 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM199 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28971	NM_152464.3 Latest version!	Homo sapiens transmembrane protein 199 (TMEM199), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu28971	NM_152464.2	Homo sapiens transmembrane protein 199 (TMEM199), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM199 ( NM_152464.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_689677.1 Homo sapiens transmembrane protein 199 (TMEM199), mRNA.

Price & Availability↑

CloneID	OHu28971
Clone ID Related Accession (Same CDS sequence)	NM_152464.2 , NM_152464.3
Accession Version	NM_152464.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-13
Organism	Homo sapiens(human)
Product	transmembrane protein 199
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC327155.1, BC033113.1, AC002094.1, BQ891740.1 and AA279827.1. This sequence is a reference standard in the RefSeqGene project. On Jan 13, 2015 this sequence version replaced NM_152464.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.228339.1, SRR1803612.284294.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCT CTTTGCTTGC GGGCGAGCGA TTGGTGCGTG CTTTGGGCCC CGGCGGGGAG 
CTGGAGCCAG AGCGGCTACC CCGAAAGCTG CGGGCCGAGC TTGAGGCCGC GCTGGGGAAG 
AAGCACAAGG GCGGTGATAG CTCCAGTGGC CCCCAACGCT TGGTTTCTTT CCGTCTCATC 
CGGGATCTGC ACCAGCATCT GAGAGAAAGG GATTCCAAAC TATACCTCCA TGAGCTCCTA 
GAAGGCAGTG AAATCTATCT CCCAGAGGTT GTGAAGCCTC CACGGAACCC AGAACTAGTT 
GCCCGGCTGG AGAAGATTAA GATACAGCTG GCCAATGAGG AATATAAACG GATCACCCGC 
AACGTCACTT GTCAGGATAC AAGACATGGT GGGACTCTCA GCGACCTGGG AAAGCAAGTG 
AGATCATTGA AGGCTCTGGT CATCACCATC TTCAATTTCA TTGTCACGGT GGTTGCTGCC 
TTCGTCTGCA CTTACCTTGG AAGCCAATAT ATCTTCACAG AAATGGCCTC GCGGGTGCTA 
GCTGCATTGA TCGTCGCCTC TGTGGTGGGT CTGGCCGAGC TGTATGTCAT GGTGCGGGCA 
ATGGAAGGCG AGCTGGGAGA ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689677.1
CDS	91..717
Translation	MASSLLAGERLVRALGPGGELEPERLPRKLRAELEAALGKKHKGGDSSSGPQRLVSFRLIRDLHQHLRERDSKLYLHELLEGSEIYLPEVVKPPRNPELVARLEKIKIQLANEEYKRITRNVTCQDTRHGGTLSDLGKQVRSLKALVITIFNFIVTVVAAFVCTYLGSQYIFTEMASRVLAALIVASVVGLAELYVMVRAMEGELGEL

Target ORF information:

RefSeq Version	NM_152464.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 199 (TMEM199), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152464.2

ORF Insert Sequence:

ATGGCGTCCT CTTTGCTTGC GGGCGAGCGA TTGGTGCGTG CTTTGGGCCC CGGCGGGGAG 
CTGGAGCCAG AGCGGCTACC CCGAAAGCTG CGGGCCGAGC TTGAGGCCGC GCTGGGGAAG 
AAGCACAAGG GCGGTGATAG CTCCAGTGGC CCCCAACGCT TGGTTTCTTT CCGTCTCATC 
CGGGATCTGC ACCAGCATCT GAGAGAAAGG GATTCCAAAC TATACCTCCA TGAGCTCCTA 
GAAGGCAGTG AAATCTATCT CCCAGAGGTT GTGAAGCCTC CACGGAACCC AGAACTAGTT 
GCCCGGCTGG AGAAGATTAA GATACAGCTG GCCAATGAGG AATATAAACG GATCACCCGC 
AACGTCACTT GTCAGGATAC AAGACATGGT GGGACTCTCA GCGACCTGGG AAAGCAAGTG 
AGATCATTGA AGGCTCTGGT CATCACCATC TTCAATTTCA TTGTCACGGT GGTTGCTGCC 
TTCGTCTGCA CTTACCTTGG AAGCCAATAT ATCTTCACAG AAATGGCCTC GCGGGTGCTA 
GCTGCATTGA TCGTCGCCTC TGTGGTGGGT CTGGCCGAGC TGTATGTCAT GGTGCGGGCA 
ATGGAAGGCG AGCTGGGAGA ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM199 ( NM_152464.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689677.1 Homo sapiens transmembrane protein 199 (TMEM199), mRNA.

Price & Availability↑

CloneID	OHu28971
Clone ID Related Accession (Same CDS sequence)	NM_152464.2 , NM_152464.3
Accession Version	NM_152464.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	transmembrane protein 199
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC327155.1, BC033113.1, AC002094.1, BQ891740.1 and AA279827.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_152464.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.168609.1, SRR1803614.228339.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000292114.8/ ENSP00000292114.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGTCCT CTTTGCTTGC GGGCGAGCGA TTGGTGCGTG CTTTGGGCCC CGGCGGGGAG 
CTGGAGCCAG AGCGGCTACC CCGAAAGCTG CGGGCCGAGC TTGAGGCCGC GCTGGGGAAG 
AAGCACAAGG GCGGTGATAG CTCCAGTGGC CCCCAACGCT TGGTTTCTTT CCGTCTCATC 
CGGGATCTGC ACCAGCATCT GAGAGAAAGG GATTCCAAAC TATACCTCCA TGAGCTCCTA 
GAAGGCAGTG AAATCTATCT CCCAGAGGTT GTGAAGCCTC CACGGAACCC AGAACTAGTT 
GCCCGGCTGG AGAAGATTAA GATACAGCTG GCCAATGAGG AATATAAACG GATCACCCGC 
AACGTCACTT GTCAGGATAC AAGACATGGT GGGACTCTCA GCGACCTGGG AAAGCAAGTG 
AGATCATTGA AGGCTCTGGT CATCACCATC TTCAATTTCA TTGTCACGGT GGTTGCTGCC 
TTCGTCTGCA CTTACCTTGG AAGCCAATAT ATCTTCACAG AAATGGCCTC GCGGGTGCTA 
GCTGCATTGA TCGTCGCCTC TGTGGTGGGT CTGGCCGAGC TGTATGTCAT GGTGCGGGCA 
ATGGAAGGCG AGCTGGGAGA ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689677.1
CDS	25..651
Translation	MASSLLAGERLVRALGPGGELEPERLPRKLRAELEAALGKKHKGGDSSSGPQRLVSFRLIRDLHQHLRERDSKLYLHELLEGSEIYLPEVVKPPRNPELVARLEKIKIQLANEEYKRITRNVTCQDTRHGGTLSDLGKQVRSLKALVITIFNFIVTVVAAFVCTYLGSQYIFTEMASRVLAALIVASVVGLAELYVMVRAMEGELGEL

Target ORF information:

RefSeq Version	NM_152464.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 199 (TMEM199), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152464.3

ORF Insert Sequence:

ATGGCGTCCT CTTTGCTTGC GGGCGAGCGA TTGGTGCGTG CTTTGGGCCC CGGCGGGGAG 
CTGGAGCCAG AGCGGCTACC CCGAAAGCTG CGGGCCGAGC TTGAGGCCGC GCTGGGGAAG 
AAGCACAAGG GCGGTGATAG CTCCAGTGGC CCCCAACGCT TGGTTTCTTT CCGTCTCATC 
CGGGATCTGC ACCAGCATCT GAGAGAAAGG GATTCCAAAC TATACCTCCA TGAGCTCCTA 
GAAGGCAGTG AAATCTATCT CCCAGAGGTT GTGAAGCCTC CACGGAACCC AGAACTAGTT 
GCCCGGCTGG AGAAGATTAA GATACAGCTG GCCAATGAGG AATATAAACG GATCACCCGC 
AACGTCACTT GTCAGGATAC AAGACATGGT GGGACTCTCA GCGACCTGGG AAAGCAAGTG 
AGATCATTGA AGGCTCTGGT CATCACCATC TTCAATTTCA TTGTCACGGT GGTTGCTGCC 
TTCGTCTGCA CTTACCTTGG AAGCCAATAT ATCTTCACAG AAATGGCCTC GCGGGTGCTA 
GCTGCATTGA TCGTCGCCTC TGTGGTGGGT CTGGCCGAGC TGTATGTCAT GGTGCGGGCA 
ATGGAAGGCG AGCTGGGAGA ACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels.
eLife6(2017 03)
Miles AL,Burr SP,Grice GL,Nathan JA

TMEM199 Deficiency Is a Disorder of Golgi Homeostasis Characterized by Elevated Aminotransferases, Alkaline Phosphatase, and Cholesterol and Abnormal Glycosylation.
American journal of human genetics98(2)322-30(2016 Feb)
Jansen JC,Timal S,van Scherpenzeel M,Michelakakis H,Vicogne D,Ashikov A,Moraitou M,Hoischen A,Huijben K,Steenbergen G,van den Boogert MA,Porta F,Calvo PL,Mavrikou M,Cenacchi G,van den Bogaart G,Salomon J,Holleboom AG,Rodenburg RJ,Drenth JP,Huynen MA,Wevers RA,Morava E,Foulquier F,Veltman JA,Lefeber DJ

Meta-analysis of genome-wide association studies identifies two loci associated with circulating osteoprotegerin levels.
Human molecular genetics23(24)6684-93(2014 Dec)
Kwan JS,Hsu YH,Cheung CL,Dupuis J,Saint-Pierre A,Eriksson J,Handelman SK,Aragaki A,Karasik D,Pramstaller PP,Kooperberg C,Lacroix AZ,Larson MG,Lau KS,Lorentzon M,Pichler I,Sham PC,Taliun D,Vandenput L,Kiel DP,Hicks AA,Jackson RD,Ohlsson C,Benjamin EJ,Kung AW

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TMEM199 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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