CSPG4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CSPG4
Entrez Gene ID	1464
Full Name	chondroitin sulfate proteoglycan 4
Synonyms	HMW-MAA,MCSP,MCSPG,MEL-CSPG,MSK16,NG2
General protein information	Preferred Names chondroitin sulfate proteoglycan 4 Names chondroitin sulfate proteoglycan 4 chondroitin sulfate proteoglycan 4 (melanoma-associated) chondroitin sulfate proteoglycan NG2 melanoma chondroitin sulfate proteoglycan melanoma-associated chondroitin sulfate proteoglycan
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q24.2
Summary	A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008].
Disorder MIM:	601172

mRNA and Protein(s)

mRNA	Protein	Name
NM_001897.4	NP_001888.2	chondroitin sulfate proteoglycan 4 precursor
NM_001897.5	NP_001888.2	chondroitin sulfate proteoglycan 4 precursor

Pathways from BioSystems

Homologies

	CSPG4	XP_002804928.1
Canis lupus familiaris (dog)	CSPG4	XP_005638698.1
Bos taurus (cattle)	CSPG4	NP_001179711.1
Pan troglodytes (chimpanzee)	CSPG4	XP_001144835.3
Mus musculus (house mouse)	Cspg4	NP_620570.2
Homo sapiens (human)	CSPG4	NP_001888.2
Rattus norvegicus (Norway rat)	Cspg4	NP_112284.1
Gallus gallus (chicken)	CSPG4	XP_423277.4
Danio rerio (zebrafish)	cspg4	XP_001923457.2
Xenopus tropicalis (tropical clawed frog)	LOC101733782	XP_004916553.1

Gene Ontology
Bibliography

Related articles in PubMed

Methylation Status of Transcriptional Modulatory Genes Associated with Colorectal Cancer in Northeast China.
Gao HL, Wang X, Sun HR, Zhou JD, Lin SQ, Xing YH, Zhu L, Zhou HB, Zhao YS, Chi Q, Liu YP
Gut and liver(2018 Jan)

Effects of chondroitin sulfate proteoglycan 4 (NG2/CSPG4) on soft tissue sarcoma growth depend on tumor developmental stage.
Hsu SC, Nadesan P, Puviindran V, Stallcup WB, Kirsch DG, Alman BA
The Journal of biological chemistry(2017 Dec)

NG2/CSPG4 and progranulin in the posttraumatic glial scar.
Sch?fer MKE, Tegeder I
Matrix biology : journal of the International Society for Matrix Biology(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In addition to establishing the binding region for CSPG4, this work ascribes for the first time a role in TcdB CROPs in receptor binding and further clarifies the relative roles of host receptors in TcdB pathogenesis.
Title: Functional defects in

of NG2/CSPG4 rather than changes in CD44 or Ki-67 expression is associated with low overall survival in glioblastoma multiforme patients
Title: Prognostic relevance of NG2/CSPG4, CD44 and Ki-67 in patients with glioblastoma.

CSPG4 is a cell surface proteoglycan regulated by interleukin 11 in human endometrial epithelial cancer cells. the data suggest that CSPG4 inhibition may impair endometrial cancer progression by reducing cancer cell proliferation, migration and potentially epithelial-to-mesenchymal transition.
Title: Chondroitin sulfate proteoglycan protein is stimulated by interleukin?11 and promotes endometrial epithelial cancer cell proliferation and migration.

The results indicate that CSPG4/NG2 has roles in regulating chondrosarcoma cell function in relation to growth, spread and resistance to chemotherapy.
Title: Functional roles of CSPG4/NG2 in chondrosarcoma.

the ratio of serum proteoglycan 4 to protease C1 inhibitor may be used for screening of early breast cancer.
Title: Unmasking Heavily O-Glycosylated Serum Proteins Using Perchloric Acid: Identification of Serum Proteoglycan 4 and Protease C1 Inhibitor as Molecular Indicators for Screening of Breast Cancer.

The following CSPG4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CSPG4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13281	NM_001897.5 Latest version!	Homo sapiens chondroitin sulfate proteoglycan 4 (CSPG4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
View Old Accession Versions >>
OHu13281	NM_001897.4	Homo sapiens chondroitin sulfate proteoglycan 4 (CSPG4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CSPG4 ( NM_001897.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001888.2 Homo sapiens chondroitin sulfate proteoglycan 4 (CSPG4), mRNA.

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CloneID	OHu13281
Clone ID Related Accession (Same CDS sequence)	NM_001897.4 , NM_001897.5
Accession Version	NM_001897.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Homo sapiens(human)
Product	chondroitin sulfate proteoglycan 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP363063.1, AY359468.1, X96753.1, BQ679485.1, CA428796.1 and CA438367.1. On Feb 23, 2007 this sequence version replaced NM_001897.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X96753.1, AY359468.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTCCG GGCCGCGGCC CCCACTTCCA GCCCCCGGCC TGGCCTTGGC TTTGACCCTG 
ACTATGTTGG CCAGACTTGC ATCCGCGGCT TCCTTCTTCG GTGAGAACCA CCTGGAGGTG 
CCTGTGGCCA CGGCTCTGAC CGACATAGAC CTGCAGCTGC AGTTCTCCAC GTCCCAGCCC 
GAAGCCCTCC TTCTCCTGGC AGCAGGCCCA GCTGACCACC TCCTGCTGCA GCTCTACTCT 
GGACGCCTGC AGGTCAGACT TGTTCTGGGC CAGGAGGAGC TGAGGCTGCA GACTCCAGCA 
GAGACGCTGC TGAGTGACTC CATCCCCCAC ACTGTGGTGC TGACTGTCGT AGAGGGCTGG 
GCCACGTTGT CAGTCGATGG GTTTCTGAAC GCCTCCTCAG CAGTCCCAGG AGCCCCCCTA 
GAGGTCCCCT ATGGGCTCTT TGTTGGGGGC ACTGGGACCC TTGGCCTGCC CTACCTGAGG 
GGAACCAGCC GACCCCTGAG GGGTTGCCTC CATGCAGCCA CCCTCAATGG CCGCAGCCTC 
CTCCGGCCTC TGACCCCCGA TGTGCATGAG GGCTGTGCTG AAGAGTTTTC TGCCAGTGAT 
GATGTGGCCC TGGGCTTCTC TGGGCCCCAC TCTCTGGCTG CCTTCCCTGC CTGGGGCACT 
CAGGACGAAG GAACCCTAGA GTTTACACTC ACCACACAGA GCCGGCAGGC ACCCTTGGCC 
TTCCAGGCAG GGGGCCGGCG TGGGGACTTC ATCTATGTGG ACATATTTGA GGGCCACCTG 
CGGGCCGTGG TGGAGAAGGG CCAGGGTACC GTATTGCTCC ACAACAGTGT GCCTGTGGCC 
GATGGGCAGC CCCATGAGGT CAGTGTCCAC ATCAATGCTC ACCGGCTGGA AATCTCCGTG 
GACCAGTACC CTACGCATAC TTCGAACCGA GGAGTCCTCA GCTACCTGGA GCCACGGGGC 
AGTCTCCTTC TCGGGGGGCT GGATGCAGAG GCCTCTCGTC ACCTCCAGGA ACACCGCCTG 
GGCCTGACAC CAGAGGCCAC CAATGCCTCC CTGCTGGGCT GCATGGAAGA CCTCAGTGTC 
AATGGCCAGA GGCGGGGGCT GCGGGAAGCT TTGCTGACGC GCAACATGGC AGCCGGCTGC 
AGGCTGGAGG AGGAGGAGTA TGAGGACGAT GCCTATGGAC ATTATGAAGC TTTCTCCACC 
CTGGCCCCTG AGGCTTGGCC AGCCATGGAG CTGCCTGAGC CATGCGTGCC TGAGCCAGGG 
CTGCCTCCTG TCTTTGCCAA TTTCACCCAG CTGCTGACTA TCAGCCCACT GGTGGTGGCC 
GAGGGGGGCA CAGCCTGGCT TGAGTGGAGG CATGTGCAGC CCACGCTGGA CCTGATGGAG 
GCTGAGCTGC GCAAATCCCA GGTGCTGTTC AGCGTGACCC GAGGGGCACG CCATGGCGAG 
CTCGAGCTGG ACATCCCGGG AGCCCAGGCA CGAAAAATGT TCACCCTCCT GGACGTGGTG 
AACCGCAAGG CCCGCTTCAT CCACGATGGC TCTGAGGACA CCTCCGACCA GCTGGTGCTG 
GAGGTGTCGG TGACGGCTCG GGTGCCCATG CCCTCATGCC TTCGGAGGGG CCAAACATAC 
CTCCTGCCCA TCCAGGTCAA CCCTGTCAAT GACCCACCCC ACATCATCTT CCCACATGGC 
AGCCTCATGG TGATCCTGGA ACACACGCAG AAGCCGCTGG GGCCTGAGGT TTTCCAGGCC 
TATGACCCGG ACTCTGCCTG TGAGGGCCTC ACCTTCCAGG TCCTTGGCAC CTCCTCTGGC 
CTCCCCGTGG AGCGCCGAGA CCAGCCTGGG GAGCCGGCGA CCGAGTTCTC CTGCCGGGAG 
TTGGAGGCCG GCAGCCTAGT CTATGTCCAC CGCGGTGGTC CTGCACAGGA CTTGACGTTC 
CGGGTCAGCG ATGGACTGCA GGCCAGCCCC CCGGCCACGC TGAAGGTGGT GGCCATCCGG 
CCGGCCATAC AGATCCACCG CAGCACAGGG TTGCGACTGG CCCAAGGCTC TGCCATGCCC 
ATCTTGCCCG CCAACCTGTC GGTGGAGACC AATGCCGTGG GGCAGGATGT GAGCGTGCTG 
TTCCGCGTCA CTGGGGCCCT GCAGTTTGGG GAGCTGCAGA AGCAGGGGGC AGGTGGGGTG 
GAGGGTGCTG AGTGGTGGGC CACACAGGCG TTCCACCAGC GGGATGTGGA GCAGGGCCGC 
GTGAGGTACC TGAGCACTGA CCCACAGCAC CACGCTTACG ACACCGTGGA GAACCTGGCC 
CTGGAGGTGC AGGTGGGCCA GGAGATCCTG AGCAATCTGT CCTTCCCAGT GACCATCCAG 
AGAGCCACTG TGTGGATGCT GCGGCTGGAG CCACTGCACA CTCAGAACAC CCAGCAGGAG 
ACCCTCACCA CAGCCCACCT GGAGGCCACC CTGGAGGAGG CAGGCCCAAG CCCCCCAACC 
TTCCATTATG AGGTGGTTCA GGCTCCCAGG AAAGGCAACC TTCAACTACA GGGCACAAGG 
CTGTCAGATG GCCAGGGCTT CACCCAGGAT GACATACAGG CTGGCCGGGT GACCTATGGG 
GCCACAGCAC GTGCCTCAGA GGCAGTCGAG GACACCTTCC GTTTCCGTGT CACAGCTCCA 
CCATATTTCT CCCCACTCTA TACCTTCCCC ATCCACATTG GTGGTGACCC AGATGCGCCT 
GTCCTCACCA ATGTCCTCCT CGTGGTGCCT GAGGGTGGTG AGGGTGTCCT CTCTGCTGAC 
CACCTCTTTG TCAAGAGTCT CAACAGTGCC AGCTACCTCT ATGAGGTCAT GGAGCGGCCC 
CGCCATGGGA GGTTGGCTTG GCGTGGGACA CAGGACAAGA CCACTATGGT GACATCCTTC 
ACCAATGAAG ACCTGTTGCG TGGCCGGCTG GTCTACCAGC ATGATGACTC CGAGACCACA 
GAAGATGATA TCCCATTTGT TGCTACCCGC CAGGGCGAGA GCAGTGGTGA CATGGCCTGG 
GAGGAGGTAC GGGGTGTCTT CCGAGTGGCC ATCCAGCCCG TGAATGACCA CGCCCCTGTG 
CAGACCATCA GCCGGATCTT CCATGTGGCC CGGGGTGGGC GGCGGCTGCT GACTACAGAC 
GACGTGGCCT TCAGCGATGC TGACTCGGGC TTTGCTGACG CCCAGCTGGT GCTTACCCGC 
AAGGACCTCC TCTTTGGCAG TATCGTGGCC GTAGATGAGC CCACGCGGCC CATCTACCGC 
TTCACCCAGG AGGACCTCAG GAAGAGGCGA GTACTGTTCG TGCACTCAGG GGCTGACCGT 
GGCTGGATCC AGCTGCAGGT GTCCGACGGG CAACACCAGG CCACTGCGCT GCTGGAGGTG 
CAGGCCTCGG AACCCTACCT CCGTGTGGCC AACGGCTCCA GCCTTGTGGT CCCTCAAGGA 
GGCCAGGGCA CCATCGACAC GGCCGTGCTC CACCTGGACA CCAACCTCGA CATCCGCAGT 
GGGGATGAGG TCCACTACCA CGTCACAGCT GGCCCTCGCT GGGGACAGCT AGTCCGGGCT 
GGTCAGCCAG CCACAGCCTT CTCCCAGCAG GACCTGCTGG ATGGGGCCGT TCTCTATAGC 
CACAATGGCA GCCTCAGCCC CCGCGACACC ATGGCCTTCT CCGTGGAAGC AGGGCCAGTG 
CACACGGATG CCACCCTACA AGTGACCATT GCCCTAGAGG GCCCACTGGC CCCACTGAAG 
CTGGTCCGGC ACAAGAAGAT CTACGTCTTC CAGGGAGAGG CAGCTGAGAT CAGAAGGGAC 
CAGCTGGAGG CAGCCCAGGA GGCAGTGCCA CCTGCAGACA TCGTATTCTC AGTGAAGAGC 
CCACCGAGTG CCGGCTACCT GGTGATGGTG TCGCGTGGCG CCTTGGCAGA TGAGCCACCC 
AGCCTGGACC CTGTGCAGAG CTTCTCCCAG GAGGCAGTGG ACACAGGCAG GGTCCTGTAC 
CTGCACTCCC GCCCTGAGGC CTGGAGCGAT GCCTTCTCGC TGGATGTGGC CTCAGGCCTG 
GGTGCTCCCC TCGAGGGCGT CCTTGTGGAG CTGGAGGTGC TGCCCGCTGC CATCCCACTA 
GAGGCGCAAA ACTTCAGCGT CCCTGAGGGT GGCAGCCTCA CCCTGGCCCC TCCACTGCTC 
CGTGTCTCCG GGCCCTACTT CCCCACTCTC CTGGGCCTCA GCCTGCAGGT GCTGGAGCCA 
CCCCAGCATG GAGCCCTGCA GAAGGAGGAC GGACCTCAAG CCAGGACCCT CAGCGCCTTC 
TCCTGGAGAA TGGTGGAAGA GCAGCTGATC CGCTACGTGC ATGACGGGAG CGAGACACTG 
ACAGACAGTT TTGTCCTGAT GGCTAATGCC TCCGAGATGG ATCGCCAGAG CCATCCTGTG 
GCCTTCACTG TCACTGTCCT GCCTGTCAAT GACCAACCCC CCATCCTCAC TACAAACACA 
GGCCTGCAGA TGTGGGAGGG GGCCACTGCG CCCATCCCTG CGGAGGCTCT GAGGAGCACG 
GACGGCGACT CTGGGTCTGA GGATCTGGTC TACACCATCG AGCAGCCCAG CAACGGGCGG 
GTAGTGCTGC GGGGGGCGCC GGGCACTGAG GTGCGCAGCT TCACGCAGGC CCAGCTGGAC 
GGCGGGCTCG TGCTGTTCTC ACACAGAGGA ACCCTGGATG GAGGCTTCCG CTTCCGCCTC 
TCTGACGGCG AGCACACTTC CCCCGGACAC TTCTTCCGAG TGACGGCCCA GAAGCAAGTG 
CTCCTCTCGC TGAAGGGCAG CCAGACACTG ACTGTCTGCC CAGGGTCCGT CCAGCCACTC 
AGCAGTCAGA CCCTCAGGGC CAGCTCCAGC GCAGGCACTG ACCCCCAGCT CCTGCTCTAC 
CGTGTGGTGC GGGGCCCCCA GCTAGGCCGG CTGTTCCACG CCCAGCAGGA CAGCACAGGG 
GAGGCCCTGG TGAACTTCAC TCAGGCAGAG GTCTACGCTG GGAATATTCT GTATGAGCAT 
GAGATGCCCC CCGAGCCCTT TTGGGAGGCC CATGATACCC TAGAGCTCCA GCTGTCCTCG 
CCGCCTGCCC GGGACGTGGC CGCCACCCTT GCTGTGGCTG TGTCTTTTGA GGCTGCCTGT 
CCCCAGCGCC CCAGCCACCT CTGGAAGAAC AAAGGTCTCT GGGTCCCCGA GGGCCAGCGG 
GCCAGGATCA CCGTGGCTGC TCTGGATGCC TCCAATCTCT TGGCCAGCGT TCCATCACCC 
CAGCGCTCAG AGCATGATGT GCTCTTCCAG GTCACACAGT TCCCCAGCCG GGGCCAGCTG 
TTGGTGTCCG AGGAGCCCCT CCATGCTGGG CAGCCCCACT TCCTGCAGTC CCAGCTGGCT 
GCAGGGCAGC TAGTGTATGC CCACGGCGGT GGGGGCACCC AGCAGGATGG CTTCCACTTT 
CGTGCCCACC TCCAGGGGCC AGCAGGGGCC TCCGTGGCTG GACCCCAAAC CTCAGAGGCC 
TTTGCCATCA CGGTGAGGGA TGTAAATGAG CGGCCCCCTC AGCCACAGGC CTCTGTCCCA 
CTCCGGCTCA CCCGAGGCTC TCGTGCCCCC ATCTCCCGGG CCCAGCTGAG TGTGGTGGAC 
CCAGACTCAG CTCCTGGGGA GATTGAGTAC GAGGTCCAGC GGGCACCCCA CAACGGCTTC 
CTCAGCCTGG TGGGTGGTGG CCTGGGGCCC GTGACCCGCT TCACGCAAGC CGATGTGGAT 
TCAGGGCGGC TGGCCTTCGT GGCCAACGGG AGCAGCGTGG CAGGCATCTT CCAGCTGAGC 
ATGTCTGATG GGGCCAGCCC ACCCCTGCCC ATGTCCCTGG CTGTGGACAT CCTACCATCC 
GCCATCGAGG TGCAGCTGCG GGCACCCCTG GAGGTGCCCC AAGCTTTGGG GCGCTCCTCA 
CTGAGCCAGC AGCAGCTCCG GGTGGTTTCA GATCGGGAGG AGCCAGAGGC AGCATACCGC 
CTCATCCAGG GACCCCAGTA TGGGCATCTC CTGGTGGGCG GGCGGCCCAC CTCGGCCTTC 
AGCCAATTCC AGATAGACCA GGGCGAGGTG GTCTTTGCCT TCACCAACTT CTCCTCCTCT 
CATGACCACT TCAGAGTCCT GGCACTGGCT AGGGGTGTCA ATGCATCAGC CGTAGTGAAC 
GTCACTGTGA GGGCTCTGCT GCATGTGTGG GCAGGTGGGC CATGGCCCCA GGGTGCCACC 
CTGCGCCTGG ACCCCACCGT CCTAGATGCT GGCGAGCTGG CCAACCGCAC AGGCAGTGTG 
CCGCGCTTCC GCCTCCTGGA GGGACCCCGG CATGGCCGCG TGGTCCGCGT GCCCCGAGCC 
AGGACGGAGC CCGGGGGCAG CCAGCTGGTG GAGCAGTTCA CTCAGCAGGA CCTTGAGGAC 
GGGAGGCTGG GGCTGGAGGT GGGCAGGCCA GAGGGGAGGG CCCCCGGCCC CGCAGGTGAC 
AGTCTCACTC TGGAGCTGTG GGCACAGGGC GTCCCGCCTG CTGTGGCCTC CCTGGACTTT 
GCCACTGAGC CTTACAATGC TGCCCGGCCC TACAGCGTGG CCCTGCTCAG TGTCCCCGAG 
GCCGCCCGGA CGGAAGCAGG GAAGCCAGAG AGCAGCACCC CCACAGGCGA GCCAGGCCCC 
ATGGCATCCA GCCCTGAGCC CGCTGTGGCC AAGGGAGGCT TCCTGAGCTT CCTTGAGGCC 
AACATGTTCA GCGTCATCAT CCCCATGTGC CTGGTACTTC TGCTCCTGGC GCTCATCCTG 
CCCCTGCTCT TCTACCTCCG AAAACGCAAC AAGACGGGCA AGCATGACGT CCAGGTCCTG 
ACTGCCAAGC CCCGCAACGG CCTGGCTGGT GACACCGAGA CCTTTCGCAA GGTGGAGCCA 
GGCCAGGCCA TCCCGCTCAC AGCTGTGCCT GGCCAGGGGC CCCCTCCAGG AGGCCAGCCT 
GACCCAGAGC TGCTGCAGTT CTGCCGGACA CCCAACCCTG CCCTTAAGAA TGGCCAGTAC 
TGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001888.2
CDS	94..7062
Translation	MQSGPRPPLPAPGLALALTLTMLARLASAASFFGENHLEVPVATALTDIDLQLQFSTSQPEALLLLAAGPADHLLLQLYSGRLQVRLVLGQEELRLQTPAETLLSDSIPHTVVLTVVEGWATLSVDGFLNASSAVPGAPLEVPYGLFVGGTGTLGLPYLRGTSRPLRGCLHAATLNGRSLLRPLTPDVHEGCAEEFSASDDVALGFSGPHSLAAFPAWGTQDEGTLEFTLTTQSRQAPLAFQAGGRRGDFIYVDIFEGHLRAVVEKGQGTVLLHNSVPVADGQPHEVSVHINAHRLEISVDQYPTHTSNRGVLSYLEPRGSLLLGGLDAEASRHLQEHRLGLTPEATNASLLGCMEDLSVNGQRRGLREALLTRNMAAGCRLEEEEYEDDAYGHYEAFSTLAPEAWPAMELPEPCVPEPGLPPVFANFTQLLTISPLVVAEGGTAWLEWRHVQPTLDLMEAELRKSQVLFSVTRGARHGELELDIPGAQARKMFTLLDVVNRKARFIHDGSEDTSDQLVLEVSVTARVPMPSCLRRGQTYLLPIQVNPVNDPPHIIFPHGSLMVILEHTQKPLGPEVFQAYDPDSACEGLTFQVLGTSSGLPVERRDQPGEPATEFSCRELEAGSLVYVHRGGPAQDLTFRVSDGLQASPPATLKVVAIRPAIQIHRSTGLRLAQGSAMPILPANLSVETNAVGQDVSVLFRVTGALQFGELQKQGAGGVEGAEWWATQAFHQRDVEQGRVRYLSTDPQHHAYDTVENLALEVQVGQEILSNLSFPVTIQRATVWMLRLEPLHTQNTQQETLTTAHLEATLEEAGPSPPTFHYEVVQAPRKGNLQLQGTRLSDGQGFTQDDIQAGRVTYGATARASEAVEDTFRFRVTAPPYFSPLYTFPIHIGGDPDAPVLTNVLLVVPEGGEGVLSADHLFVKSLNSASYLYEVMERPRHGRLAWRGTQDKTTMVTSFTNEDLLRGRLVYQHDDSETTEDDIPFVATRQGESSGDMAWEEVRGVFRVAIQPVNDHAPVQTISRIFHVARGGRRLLTTDDVAFSDADSGFADAQLVLTRKDLLFGSIVAVDEPTRPIYRFTQEDLRKRRVLFVHSGADRGWIQLQVSDGQHQATALLEVQASEPYLRVANGSSLVVPQGGQGTIDTAVLHLDTNLDIRSGDEVHYHVTAGPRWGQLVRAGQPATAFSQQDLLDGAVLYSHNGSLSPRDTMAFSVEAGPVHTDATLQVTIALEGPLAPLKLVRHKKIYVFQGEAAEIRRDQLEAAQEAVPPADIVFSVKSPPSAGYLVMVSRGALADEPPSLDPVQSFSQEAVDTGRVLYLHSRPEAWSDAFSLDVASGLGAPLEGVLVELEVLPAAIPLEAQNFSVPEGGSLTLAPPLLRVSGPYFPTLLGLSLQVLEPPQHGALQKEDGPQARTLSAFSWRMVEEQLIRYVHDGSETLTDSFVLMANASEMDRQSHPVAFTVTVLPVNDQPPILTTNTGLQMWEGATAPIPAEALRSTDGDSGSEDLVYTIEQPSNGRVVLRGAPGTEVRSFTQAQLDGGLVLFSHRGTLDGGFRFRLSDGEHTSPGHFFRVTAQKQVLLSLKGSQTLTVCPGSVQPLSSQTLRASSSAGTDPQLLLYRVVRGPQLGRLFHAQQDSTGEALVNFTQAEVYAGNILYEHEMPPEPFWEAHDTLELQLSSPPARDVAATLAVAVSFEAACPQRPSHLWKNKGLWVPEGQRARITVAALDASNLLASVPSPQRSEHDVLFQVTQFPSRGQLLVSEEPLHAGQPHFLQSQLAAGQLVYAHGGGGTQQDGFHFRAHLQGPAGASVAGPQTSEAFAITVRDVNERPPQPQASVPLRLTRGSRAPISRAQLSVVDPDSAPGEIEYEVQRAPHNGFLSLVGGGLGPVTRFTQADVDSGRLAFVANGSSVAGIFQLSMSDGASPPLPMSLAVDILPSAIEVQLRAPLEVPQALGRSSLSQQQLRVVSDREEPEAAYRLIQGPQYGHLLVGGRPTSAFSQFQIDQGEVVFAFTNFSSSHDHFRVLALARGVNASAVVNVTVRALLHVWAGGPWPQGATLRLDPTVLDAGELANRTGSVPRFRLLEGPRHGRVVRVPRARTEPGGSQLVEQFTQQDLEDGRLGLEVGRPEGRAPGPAGDSLTLELWAQGVPPAVASLDFATEPYNAARPYSVALLSVPEAARTEAGKPESSTPTGEPGPMASSPEPAVAKGGFLSFLEANMFSVIIPMCLVLLLLALILPLLFYLRKRNKTGKHDVQVLTAKPRNGLAGDTETFRKVEPGQAIPLTAVPGQGPPPGGQPDPELLQFCRTPNPALKNGQYWV

Target ORF information:

RefSeq Version	NM_001897.4
Organism	Homo sapiens(human)
Definition	Homo sapiens chondroitin sulfate proteoglycan 4 (CSPG4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001897.4

ORF Insert Sequence:

ATGCAGTCCG GGCCGCGGCC CCCACTTCCA GCCCCCGGCC TGGCCTTGGC TTTGACCCTG 
ACTATGTTGG CCAGACTTGC ATCCGCGGCT TCCTTCTTCG GTGAGAACCA CCTGGAGGTG 
CCTGTGGCCA CGGCTCTGAC CGACATAGAC CTGCAGCTGC AGTTCTCCAC GTCCCAGCCC 
GAAGCCCTCC TTCTCCTGGC AGCAGGCCCA GCTGACCACC TCCTGCTGCA GCTCTACTCT 
GGACGCCTGC AGGTCAGACT TGTTCTGGGC CAGGAGGAGC TGAGGCTGCA GACTCCAGCA 
GAGACGCTGC TGAGTGACTC CATCCCCCAC ACTGTGGTGC TGACTGTCGT AGAGGGCTGG 
GCCACGTTGT CAGTCGATGG GTTTCTGAAC GCCTCCTCAG CAGTCCCAGG AGCCCCCCTA 
GAGGTCCCCT ATGGGCTCTT TGTTGGGGGC ACTGGGACCC TTGGCCTGCC CTACCTGAGG 
GGAACCAGCC GACCCCTGAG GGGTTGCCTC CATGCAGCCA CCCTCAATGG CCGCAGCCTC 
CTCCGGCCTC TGACCCCCGA TGTGCATGAG GGCTGTGCTG AAGAGTTTTC TGCCAGTGAT 
GATGTGGCCC TGGGCTTCTC TGGGCCCCAC TCTCTGGCTG CCTTCCCTGC CTGGGGCACT 
CAGGACGAAG GAACCCTAGA GTTTACACTC ACCACACAGA GCCGGCAGGC ACCCTTGGCC 
TTCCAGGCAG GGGGCCGGCG TGGGGACTTC ATCTATGTGG ACATATTTGA GGGCCACCTG 
CGGGCCGTGG TGGAGAAGGG CCAGGGTACC GTATTGCTCC ACAACAGTGT GCCTGTGGCC 
GATGGGCAGC CCCATGAGGT CAGTGTCCAC ATCAATGCTC ACCGGCTGGA AATCTCCGTG 
GACCAGTACC CTACGCATAC TTCGAACCGA GGAGTCCTCA GCTACCTGGA GCCACGGGGC 
AGTCTCCTTC TCGGGGGGCT GGATGCAGAG GCCTCTCGTC ACCTCCAGGA ACACCGCCTG 
GGCCTGACAC CAGAGGCCAC CAATGCCTCC CTGCTGGGCT GCATGGAAGA CCTCAGTGTC 
AATGGCCAGA GGCGGGGGCT GCGGGAAGCT TTGCTGACGC GCAACATGGC AGCCGGCTGC 
AGGCTGGAGG AGGAGGAGTA TGAGGACGAT GCCTATGGAC ATTATGAAGC TTTCTCCACC 
CTGGCCCCTG AGGCTTGGCC AGCCATGGAG CTGCCTGAGC CATGCGTGCC TGAGCCAGGG 
CTGCCTCCTG TCTTTGCCAA TTTCACCCAG CTGCTGACTA TCAGCCCACT GGTGGTGGCC 
GAGGGGGGCA CAGCCTGGCT TGAGTGGAGG CATGTGCAGC CCACGCTGGA CCTGATGGAG 
GCTGAGCTGC GCAAATCCCA GGTGCTGTTC AGCGTGACCC GAGGGGCACG CCATGGCGAG 
CTCGAGCTGG ACATCCCGGG AGCCCAGGCA CGAAAAATGT TCACCCTCCT GGACGTGGTG 
AACCGCAAGG CCCGCTTCAT CCACGATGGC TCTGAGGACA CCTCCGACCA GCTGGTGCTG 
GAGGTGTCGG TGACGGCTCG GGTGCCCATG CCCTCATGCC TTCGGAGGGG CCAAACATAC 
CTCCTGCCCA TCCAGGTCAA CCCTGTCAAT GACCCACCCC ACATCATCTT CCCACATGGC 
AGCCTCATGG TGATCCTGGA ACACACGCAG AAGCCGCTGG GGCCTGAGGT TTTCCAGGCC 
TATGACCCGG ACTCTGCCTG TGAGGGCCTC ACCTTCCAGG TCCTTGGCAC CTCCTCTGGC 
CTCCCCGTGG AGCGCCGAGA CCAGCCTGGG GAGCCGGCGA CCGAGTTCTC CTGCCGGGAG 
TTGGAGGCCG GCAGCCTAGT CTATGTCCAC CGCGGTGGTC CTGCACAGGA CTTGACGTTC 
CGGGTCAGCG ATGGACTGCA GGCCAGCCCC CCGGCCACGC TGAAGGTGGT GGCCATCCGG 
CCGGCCATAC AGATCCACCG CAGCACAGGG TTGCGACTGG CCCAAGGCTC TGCCATGCCC 
ATCTTGCCCG CCAACCTGTC GGTGGAGACC AATGCCGTGG GGCAGGATGT GAGCGTGCTG 
TTCCGCGTCA CTGGGGCCCT GCAGTTTGGG GAGCTGCAGA AGCAGGGGGC AGGTGGGGTG 
GAGGGTGCTG AGTGGTGGGC CACACAGGCG TTCCACCAGC GGGATGTGGA GCAGGGCCGC 
GTGAGGTACC TGAGCACTGA CCCACAGCAC CACGCTTACG ACACCGTGGA GAACCTGGCC 
CTGGAGGTGC AGGTGGGCCA GGAGATCCTG AGCAATCTGT CCTTCCCAGT GACCATCCAG 
AGAGCCACTG TGTGGATGCT GCGGCTGGAG CCACTGCACA CTCAGAACAC CCAGCAGGAG 
ACCCTCACCA CAGCCCACCT GGAGGCCACC CTGGAGGAGG CAGGCCCAAG CCCCCCAACC 
TTCCATTATG AGGTGGTTCA GGCTCCCAGG AAAGGCAACC TTCAACTACA GGGCACAAGG 
CTGTCAGATG GCCAGGGCTT CACCCAGGAT GACATACAGG CTGGCCGGGT GACCTATGGG 
GCCACAGCAC GTGCCTCAGA GGCAGTCGAG GACACCTTCC GTTTCCGTGT CACAGCTCCA 
CCATATTTCT CCCCACTCTA TACCTTCCCC ATCCACATTG GTGGTGACCC AGATGCGCCT 
GTCCTCACCA ATGTCCTCCT CGTGGTGCCT GAGGGTGGTG AGGGTGTCCT CTCTGCTGAC 
CACCTCTTTG TCAAGAGTCT CAACAGTGCC AGCTACCTCT ATGAGGTCAT GGAGCGGCCC 
CGCCATGGGA GGTTGGCTTG GCGTGGGACA CAGGACAAGA CCACTATGGT GACATCCTTC 
ACCAATGAAG ACCTGTTGCG TGGCCGGCTG GTCTACCAGC ATGATGACTC CGAGACCACA 
GAAGATGATA TCCCATTTGT TGCTACCCGC CAGGGCGAGA GCAGTGGTGA CATGGCCTGG 
GAGGAGGTAC GGGGTGTCTT CCGAGTGGCC ATCCAGCCCG TGAATGACCA CGCCCCTGTG 
CAGACCATCA GCCGGATCTT CCATGTGGCC CGGGGTGGGC GGCGGCTGCT GACTACAGAC 
GACGTGGCCT TCAGCGATGC TGACTCGGGC TTTGCTGACG CCCAGCTGGT GCTTACCCGC 
AAGGACCTCC TCTTTGGCAG TATCGTGGCC GTAGATGAGC CCACGCGGCC CATCTACCGC 
TTCACCCAGG AGGACCTCAG GAAGAGGCGA GTACTGTTCG TGCACTCAGG GGCTGACCGT 
GGCTGGATCC AGCTGCAGGT GTCCGACGGG CAACACCAGG CCACTGCGCT GCTGGAGGTG 
CAGGCCTCGG AACCCTACCT CCGTGTGGCC AACGGCTCCA GCCTTGTGGT CCCTCAAGGA 
GGCCAGGGCA CCATCGACAC GGCCGTGCTC CACCTGGACA CCAACCTCGA CATCCGCAGT 
GGGGATGAGG TCCACTACCA CGTCACAGCT GGCCCTCGCT GGGGACAGCT AGTCCGGGCT 
GGTCAGCCAG CCACAGCCTT CTCCCAGCAG GACCTGCTGG ATGGGGCCGT TCTCTATAGC 
CACAATGGCA GCCTCAGCCC CCGCGACACC ATGGCCTTCT CCGTGGAAGC AGGGCCAGTG 
CACACGGATG CCACCCTACA AGTGACCATT GCCCTAGAGG GCCCACTGGC CCCACTGAAG 
CTGGTCCGGC ACAAGAAGAT CTACGTCTTC CAGGGAGAGG CAGCTGAGAT CAGAAGGGAC 
CAGCTGGAGG CAGCCCAGGA GGCAGTGCCA CCTGCAGACA TCGTATTCTC AGTGAAGAGC 
CCACCGAGTG CCGGCTACCT GGTGATGGTG TCGCGTGGCG CCTTGGCAGA TGAGCCACCC 
AGCCTGGACC CTGTGCAGAG CTTCTCCCAG GAGGCAGTGG ACACAGGCAG GGTCCTGTAC 
CTGCACTCCC GCCCTGAGGC CTGGAGCGAT GCCTTCTCGC TGGATGTGGC CTCAGGCCTG 
GGTGCTCCCC TCGAGGGCGT CCTTGTGGAG CTGGAGGTGC TGCCCGCTGC CATCCCACTA 
GAGGCGCAAA ACTTCAGCGT CCCTGAGGGT GGCAGCCTCA CCCTGGCCCC TCCACTGCTC 
CGTGTCTCCG GGCCCTACTT CCCCACTCTC CTGGGCCTCA GCCTGCAGGT GCTGGAGCCA 
CCCCAGCATG GAGCCCTGCA GAAGGAGGAC GGACCTCAAG CCAGGACCCT CAGCGCCTTC 
TCCTGGAGAA TGGTGGAAGA GCAGCTGATC CGCTACGTGC ATGACGGGAG CGAGACACTG 
ACAGACAGTT TTGTCCTGAT GGCTAATGCC TCCGAGATGG ATCGCCAGAG CCATCCTGTG 
GCCTTCACTG TCACTGTCCT GCCTGTCAAT GACCAACCCC CCATCCTCAC TACAAACACA 
GGCCTGCAGA TGTGGGAGGG GGCCACTGCG CCCATCCCTG CGGAGGCTCT GAGGAGCACG 
GACGGCGACT CTGGGTCTGA GGATCTGGTC TACACCATCG AGCAGCCCAG CAACGGGCGG 
GTAGTGCTGC GGGGGGCGCC GGGCACTGAG GTGCGCAGCT TCACGCAGGC CCAGCTGGAC 
GGCGGGCTCG TGCTGTTCTC ACACAGAGGA ACCCTGGATG GAGGCTTCCG CTTCCGCCTC 
TCTGACGGCG AGCACACTTC CCCCGGACAC TTCTTCCGAG TGACGGCCCA GAAGCAAGTG 
CTCCTCTCGC TGAAGGGCAG CCAGACACTG ACTGTCTGCC CAGGGTCCGT CCAGCCACTC 
AGCAGTCAGA CCCTCAGGGC CAGCTCCAGC GCAGGCACTG ACCCCCAGCT CCTGCTCTAC 
CGTGTGGTGC GGGGCCCCCA GCTAGGCCGG CTGTTCCACG CCCAGCAGGA CAGCACAGGG 
GAGGCCCTGG TGAACTTCAC TCAGGCAGAG GTCTACGCTG GGAATATTCT GTATGAGCAT 
GAGATGCCCC CCGAGCCCTT TTGGGAGGCC CATGATACCC TAGAGCTCCA GCTGTCCTCG 
CCGCCTGCCC GGGACGTGGC CGCCACCCTT GCTGTGGCTG TGTCTTTTGA GGCTGCCTGT 
CCCCAGCGCC CCAGCCACCT CTGGAAGAAC AAAGGTCTCT GGGTCCCCGA GGGCCAGCGG 
GCCAGGATCA CCGTGGCTGC TCTGGATGCC TCCAATCTCT TGGCCAGCGT TCCATCACCC 
CAGCGCTCAG AGCATGATGT GCTCTTCCAG GTCACACAGT TCCCCAGCCG GGGCCAGCTG 
TTGGTGTCCG AGGAGCCCCT CCATGCTGGG CAGCCCCACT TCCTGCAGTC CCAGCTGGCT 
GCAGGGCAGC TAGTGTATGC CCACGGCGGT GGGGGCACCC AGCAGGATGG CTTCCACTTT 
CGTGCCCACC TCCAGGGGCC AGCAGGGGCC TCCGTGGCTG GACCCCAAAC CTCAGAGGCC 
TTTGCCATCA CGGTGAGGGA TGTAAATGAG CGGCCCCCTC AGCCACAGGC CTCTGTCCCA 
CTCCGGCTCA CCCGAGGCTC TCGTGCCCCC ATCTCCCGGG CCCAGCTGAG TGTGGTGGAC 
CCAGACTCAG CTCCTGGGGA GATTGAGTAC GAGGTCCAGC GGGCACCCCA CAACGGCTTC 
CTCAGCCTGG TGGGTGGTGG CCTGGGGCCC GTGACCCGCT TCACGCAAGC CGATGTGGAT 
TCAGGGCGGC TGGCCTTCGT GGCCAACGGG AGCAGCGTGG CAGGCATCTT CCAGCTGAGC 
ATGTCTGATG GGGCCAGCCC ACCCCTGCCC ATGTCCCTGG CTGTGGACAT CCTACCATCC 
GCCATCGAGG TGCAGCTGCG GGCACCCCTG GAGGTGCCCC AAGCTTTGGG GCGCTCCTCA 
CTGAGCCAGC AGCAGCTCCG GGTGGTTTCA GATCGGGAGG AGCCAGAGGC AGCATACCGC 
CTCATCCAGG GACCCCAGTA TGGGCATCTC CTGGTGGGCG GGCGGCCCAC CTCGGCCTTC 
AGCCAATTCC AGATAGACCA GGGCGAGGTG GTCTTTGCCT TCACCAACTT CTCCTCCTCT 
CATGACCACT TCAGAGTCCT GGCACTGGCT AGGGGTGTCA ATGCATCAGC CGTAGTGAAC 
GTCACTGTGA GGGCTCTGCT GCATGTGTGG GCAGGTGGGC CATGGCCCCA GGGTGCCACC 
CTGCGCCTGG ACCCCACCGT CCTAGATGCT GGCGAGCTGG CCAACCGCAC AGGCAGTGTG 
CCGCGCTTCC GCCTCCTGGA GGGACCCCGG CATGGCCGCG TGGTCCGCGT GCCCCGAGCC 
AGGACGGAGC CCGGGGGCAG CCAGCTGGTG GAGCAGTTCA CTCAGCAGGA CCTTGAGGAC 
GGGAGGCTGG GGCTGGAGGT GGGCAGGCCA GAGGGGAGGG CCCCCGGCCC CGCAGGTGAC 
AGTCTCACTC TGGAGCTGTG GGCACAGGGC GTCCCGCCTG CTGTGGCCTC CCTGGACTTT 
GCCACTGAGC CTTACAATGC TGCCCGGCCC TACAGCGTGG CCCTGCTCAG TGTCCCCGAG 
GCCGCCCGGA CGGAAGCAGG GAAGCCAGAG AGCAGCACCC CCACAGGCGA GCCAGGCCCC 
ATGGCATCCA GCCCTGAGCC CGCTGTGGCC AAGGGAGGCT TCCTGAGCTT CCTTGAGGCC 
AACATGTTCA GCGTCATCAT CCCCATGTGC CTGGTACTTC TGCTCCTGGC GCTCATCCTG 
CCCCTGCTCT TCTACCTCCG AAAACGCAAC AAGACGGGCA AGCATGACGT CCAGGTCCTG 
ACTGCCAAGC CCCGCAACGG CCTGGCTGGT GACACCGAGA CCTTTCGCAA GGTGGAGCCA 
GGCCAGGCCA TCCCGCTCAC AGCTGTGCCT GGCCAGGGGC CCCCTCCAGG AGGCCAGCCT 
GACCCAGAGC TGCTGCAGTT CTGCCGGACA CCCAACCCTG CCCTTAAGAA TGGCCAGTAC 
TGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSPG4 ( NM_001897.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001888.2 Homo sapiens chondroitin sulfate proteoglycan 4 (CSPG4), mRNA.

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CloneID	OHu13281
Clone ID Related Accession (Same CDS sequence)	NM_001897.4 , NM_001897.5
Accession Version	NM_001897.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	chondroitin sulfate proteoglycan 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP363063.1, AY359468.1, X96753.1, BQ679485.1, CA428796.1 and CA438367.1. On Nov 22, 2018 this sequence version replaced NM_001897.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X96753.1, AY359468.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162568 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000308508.5/ ENSP00000312506.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGTCCG GGCCGCGGCC CCCACTTCCA GCCCCCGGCC TGGCCTTGGC TTTGACCCTG 
ACTATGTTGG CCAGACTTGC ATCCGCGGCT TCCTTCTTCG GTGAGAACCA CCTGGAGGTG 
CCTGTGGCCA CGGCTCTGAC CGACATAGAC CTGCAGCTGC AGTTCTCCAC GTCCCAGCCC 
GAAGCCCTCC TTCTCCTGGC AGCAGGCCCA GCTGACCACC TCCTGCTGCA GCTCTACTCT 
GGACGCCTGC AGGTCAGACT TGTTCTGGGC CAGGAGGAGC TGAGGCTGCA GACTCCAGCA 
GAGACGCTGC TGAGTGACTC CATCCCCCAC ACTGTGGTGC TGACTGTCGT AGAGGGCTGG 
GCCACGTTGT CAGTCGATGG GTTTCTGAAC GCCTCCTCAG CAGTCCCAGG AGCCCCCCTA 
GAGGTCCCCT ATGGGCTCTT TGTTGGGGGC ACTGGGACCC TTGGCCTGCC CTACCTGAGG 
GGAACCAGCC GACCCCTGAG GGGTTGCCTC CATGCAGCCA CCCTCAATGG CCGCAGCCTC 
CTCCGGCCTC TGACCCCCGA TGTGCATGAG GGCTGTGCTG AAGAGTTTTC TGCCAGTGAT 
GATGTGGCCC TGGGCTTCTC TGGGCCCCAC TCTCTGGCTG CCTTCCCTGC CTGGGGCACT 
CAGGACGAAG GAACCCTAGA GTTTACACTC ACCACACAGA GCCGGCAGGC ACCCTTGGCC 
TTCCAGGCAG GGGGCCGGCG TGGGGACTTC ATCTATGTGG ACATATTTGA GGGCCACCTG 
CGGGCCGTGG TGGAGAAGGG CCAGGGTACC GTATTGCTCC ACAACAGTGT GCCTGTGGCC 
GATGGGCAGC CCCATGAGGT CAGTGTCCAC ATCAATGCTC ACCGGCTGGA AATCTCCGTG 
GACCAGTACC CTACGCATAC TTCGAACCGA GGAGTCCTCA GCTACCTGGA GCCACGGGGC 
AGTCTCCTTC TCGGGGGGCT GGATGCAGAG GCCTCTCGTC ACCTCCAGGA ACACCGCCTG 
GGCCTGACAC CAGAGGCCAC CAATGCCTCC CTGCTGGGCT GCATGGAAGA CCTCAGTGTC 
AATGGCCAGA GGCGGGGGCT GCGGGAAGCT TTGCTGACGC GCAACATGGC AGCCGGCTGC 
AGGCTGGAGG AGGAGGAGTA TGAGGACGAT GCCTATGGAC ATTATGAAGC TTTCTCCACC 
CTGGCCCCTG AGGCTTGGCC AGCCATGGAG CTGCCTGAGC CATGCGTGCC TGAGCCAGGG 
CTGCCTCCTG TCTTTGCCAA TTTCACCCAG CTGCTGACTA TCAGCCCACT GGTGGTGGCC 
GAGGGGGGCA CAGCCTGGCT TGAGTGGAGG CATGTGCAGC CCACGCTGGA CCTGATGGAG 
GCTGAGCTGC GCAAATCCCA GGTGCTGTTC AGCGTGACCC GAGGGGCACG CCATGGCGAG 
CTCGAGCTGG ACATCCCGGG AGCCCAGGCA CGAAAAATGT TCACCCTCCT GGACGTGGTG 
AACCGCAAGG CCCGCTTCAT CCACGATGGC TCTGAGGACA CCTCCGACCA GCTGGTGCTG 
GAGGTGTCGG TGACGGCTCG GGTGCCCATG CCCTCATGCC TTCGGAGGGG CCAAACATAC 
CTCCTGCCCA TCCAGGTCAA CCCTGTCAAT GACCCACCCC ACATCATCTT CCCACATGGC 
AGCCTCATGG TGATCCTGGA ACACACGCAG AAGCCGCTGG GGCCTGAGGT TTTCCAGGCC 
TATGACCCGG ACTCTGCCTG TGAGGGCCTC ACCTTCCAGG TCCTTGGCAC CTCCTCTGGC 
CTCCCCGTGG AGCGCCGAGA CCAGCCTGGG GAGCCGGCGA CCGAGTTCTC CTGCCGGGAG 
TTGGAGGCCG GCAGCCTAGT CTATGTCCAC CGCGGTGGTC CTGCACAGGA CTTGACGTTC 
CGGGTCAGCG ATGGACTGCA GGCCAGCCCC CCGGCCACGC TGAAGGTGGT GGCCATCCGG 
CCGGCCATAC AGATCCACCG CAGCACAGGG TTGCGACTGG CCCAAGGCTC TGCCATGCCC 
ATCTTGCCCG CCAACCTGTC GGTGGAGACC AATGCCGTGG GGCAGGATGT GAGCGTGCTG 
TTCCGCGTCA CTGGGGCCCT GCAGTTTGGG GAGCTGCAGA AGCAGGGGGC AGGTGGGGTG 
GAGGGTGCTG AGTGGTGGGC CACACAGGCG TTCCACCAGC GGGATGTGGA GCAGGGCCGC 
GTGAGGTACC TGAGCACTGA CCCACAGCAC CACGCTTACG ACACCGTGGA GAACCTGGCC 
CTGGAGGTGC AGGTGGGCCA GGAGATCCTG AGCAATCTGT CCTTCCCAGT GACCATCCAG 
AGAGCCACTG TGTGGATGCT GCGGCTGGAG CCACTGCACA CTCAGAACAC CCAGCAGGAG 
ACCCTCACCA CAGCCCACCT GGAGGCCACC CTGGAGGAGG CAGGCCCAAG CCCCCCAACC 
TTCCATTATG AGGTGGTTCA GGCTCCCAGG AAAGGCAACC TTCAACTACA GGGCACAAGG 
CTGTCAGATG GCCAGGGCTT CACCCAGGAT GACATACAGG CTGGCCGGGT GACCTATGGG 
GCCACAGCAC GTGCCTCAGA GGCAGTCGAG GACACCTTCC GTTTCCGTGT CACAGCTCCA 
CCATATTTCT CCCCACTCTA TACCTTCCCC ATCCACATTG GTGGTGACCC AGATGCGCCT 
GTCCTCACCA ATGTCCTCCT CGTGGTGCCT GAGGGTGGTG AGGGTGTCCT CTCTGCTGAC 
CACCTCTTTG TCAAGAGTCT CAACAGTGCC AGCTACCTCT ATGAGGTCAT GGAGCGGCCC 
CGCCATGGGA GGTTGGCTTG GCGTGGGACA CAGGACAAGA CCACTATGGT GACATCCTTC 
ACCAATGAAG ACCTGTTGCG TGGCCGGCTG GTCTACCAGC ATGATGACTC CGAGACCACA 
GAAGATGATA TCCCATTTGT TGCTACCCGC CAGGGCGAGA GCAGTGGTGA CATGGCCTGG 
GAGGAGGTAC GGGGTGTCTT CCGAGTGGCC ATCCAGCCCG TGAATGACCA CGCCCCTGTG 
CAGACCATCA GCCGGATCTT CCATGTGGCC CGGGGTGGGC GGCGGCTGCT GACTACAGAC 
GACGTGGCCT TCAGCGATGC TGACTCGGGC TTTGCTGACG CCCAGCTGGT GCTTACCCGC 
AAGGACCTCC TCTTTGGCAG TATCGTGGCC GTAGATGAGC CCACGCGGCC CATCTACCGC 
TTCACCCAGG AGGACCTCAG GAAGAGGCGA GTACTGTTCG TGCACTCAGG GGCTGACCGT 
GGCTGGATCC AGCTGCAGGT GTCCGACGGG CAACACCAGG CCACTGCGCT GCTGGAGGTG 
CAGGCCTCGG AACCCTACCT CCGTGTGGCC AACGGCTCCA GCCTTGTGGT CCCTCAAGGA 
GGCCAGGGCA CCATCGACAC GGCCGTGCTC CACCTGGACA CCAACCTCGA CATCCGCAGT 
GGGGATGAGG TCCACTACCA CGTCACAGCT GGCCCTCGCT GGGGACAGCT AGTCCGGGCT 
GGTCAGCCAG CCACAGCCTT CTCCCAGCAG GACCTGCTGG ATGGGGCCGT TCTCTATAGC 
CACAATGGCA GCCTCAGCCC CCGCGACACC ATGGCCTTCT CCGTGGAAGC AGGGCCAGTG 
CACACGGATG CCACCCTACA AGTGACCATT GCCCTAGAGG GCCCACTGGC CCCACTGAAG 
CTGGTCCGGC ACAAGAAGAT CTACGTCTTC CAGGGAGAGG CAGCTGAGAT CAGAAGGGAC 
CAGCTGGAGG CAGCCCAGGA GGCAGTGCCA CCTGCAGACA TCGTATTCTC AGTGAAGAGC 
CCACCGAGTG CCGGCTACCT GGTGATGGTG TCGCGTGGCG CCTTGGCAGA TGAGCCACCC 
AGCCTGGACC CTGTGCAGAG CTTCTCCCAG GAGGCAGTGG ACACAGGCAG GGTCCTGTAC 
CTGCACTCCC GCCCTGAGGC CTGGAGCGAT GCCTTCTCGC TGGATGTGGC CTCAGGCCTG 
GGTGCTCCCC TCGAGGGCGT CCTTGTGGAG CTGGAGGTGC TGCCCGCTGC CATCCCACTA 
GAGGCGCAAA ACTTCAGCGT CCCTGAGGGT GGCAGCCTCA CCCTGGCCCC TCCACTGCTC 
CGTGTCTCCG GGCCCTACTT CCCCACTCTC CTGGGCCTCA GCCTGCAGGT GCTGGAGCCA 
CCCCAGCATG GAGCCCTGCA GAAGGAGGAC GGACCTCAAG CCAGGACCCT CAGCGCCTTC 
TCCTGGAGAA TGGTGGAAGA GCAGCTGATC CGCTACGTGC ATGACGGGAG CGAGACACTG 
ACAGACAGTT TTGTCCTGAT GGCTAATGCC TCCGAGATGG ATCGCCAGAG CCATCCTGTG 
GCCTTCACTG TCACTGTCCT GCCTGTCAAT GACCAACCCC CCATCCTCAC TACAAACACA 
GGCCTGCAGA TGTGGGAGGG GGCCACTGCG CCCATCCCTG CGGAGGCTCT GAGGAGCACG 
GACGGCGACT CTGGGTCTGA GGATCTGGTC TACACCATCG AGCAGCCCAG CAACGGGCGG 
GTAGTGCTGC GGGGGGCGCC GGGCACTGAG GTGCGCAGCT TCACGCAGGC CCAGCTGGAC 
GGCGGGCTCG TGCTGTTCTC ACACAGAGGA ACCCTGGATG GAGGCTTCCG CTTCCGCCTC 
TCTGACGGCG AGCACACTTC CCCCGGACAC TTCTTCCGAG TGACGGCCCA GAAGCAAGTG 
CTCCTCTCGC TGAAGGGCAG CCAGACACTG ACTGTCTGCC CAGGGTCCGT CCAGCCACTC 
AGCAGTCAGA CCCTCAGGGC CAGCTCCAGC GCAGGCACTG ACCCCCAGCT CCTGCTCTAC 
CGTGTGGTGC GGGGCCCCCA GCTAGGCCGG CTGTTCCACG CCCAGCAGGA CAGCACAGGG 
GAGGCCCTGG TGAACTTCAC TCAGGCAGAG GTCTACGCTG GGAATATTCT GTATGAGCAT 
GAGATGCCCC CCGAGCCCTT TTGGGAGGCC CATGATACCC TAGAGCTCCA GCTGTCCTCG 
CCGCCTGCCC GGGACGTGGC CGCCACCCTT GCTGTGGCTG TGTCTTTTGA GGCTGCCTGT 
CCCCAGCGCC CCAGCCACCT CTGGAAGAAC AAAGGTCTCT GGGTCCCCGA GGGCCAGCGG 
GCCAGGATCA CCGTGGCTGC TCTGGATGCC TCCAATCTCT TGGCCAGCGT TCCATCACCC 
CAGCGCTCAG AGCATGATGT GCTCTTCCAG GTCACACAGT TCCCCAGCCG GGGCCAGCTG 
TTGGTGTCCG AGGAGCCCCT CCATGCTGGG CAGCCCCACT TCCTGCAGTC CCAGCTGGCT 
GCAGGGCAGC TAGTGTATGC CCACGGCGGT GGGGGCACCC AGCAGGATGG CTTCCACTTT 
CGTGCCCACC TCCAGGGGCC AGCAGGGGCC TCCGTGGCTG GACCCCAAAC CTCAGAGGCC 
TTTGCCATCA CGGTGAGGGA TGTAAATGAG CGGCCCCCTC AGCCACAGGC CTCTGTCCCA 
CTCCGGCTCA CCCGAGGCTC TCGTGCCCCC ATCTCCCGGG CCCAGCTGAG TGTGGTGGAC 
CCAGACTCAG CTCCTGGGGA GATTGAGTAC GAGGTCCAGC GGGCACCCCA CAACGGCTTC 
CTCAGCCTGG TGGGTGGTGG CCTGGGGCCC GTGACCCGCT TCACGCAAGC CGATGTGGAT 
TCAGGGCGGC TGGCCTTCGT GGCCAACGGG AGCAGCGTGG CAGGCATCTT CCAGCTGAGC 
ATGTCTGATG GGGCCAGCCC ACCCCTGCCC ATGTCCCTGG CTGTGGACAT CCTACCATCC 
GCCATCGAGG TGCAGCTGCG GGCACCCCTG GAGGTGCCCC AAGCTTTGGG GCGCTCCTCA 
CTGAGCCAGC AGCAGCTCCG GGTGGTTTCA GATCGGGAGG AGCCAGAGGC AGCATACCGC 
CTCATCCAGG GACCCCAGTA TGGGCATCTC CTGGTGGGCG GGCGGCCCAC CTCGGCCTTC 
AGCCAATTCC AGATAGACCA GGGCGAGGTG GTCTTTGCCT TCACCAACTT CTCCTCCTCT 
CATGACCACT TCAGAGTCCT GGCACTGGCT AGGGGTGTCA ATGCATCAGC CGTAGTGAAC 
GTCACTGTGA GGGCTCTGCT GCATGTGTGG GCAGGTGGGC CATGGCCCCA GGGTGCCACC 
CTGCGCCTGG ACCCCACCGT CCTAGATGCT GGCGAGCTGG CCAACCGCAC AGGCAGTGTG 
CCGCGCTTCC GCCTCCTGGA GGGACCCCGG CATGGCCGCG TGGTCCGCGT GCCCCGAGCC 
AGGACGGAGC CCGGGGGCAG CCAGCTGGTG GAGCAGTTCA CTCAGCAGGA CCTTGAGGAC 
GGGAGGCTGG GGCTGGAGGT GGGCAGGCCA GAGGGGAGGG CCCCCGGCCC CGCAGGTGAC 
AGTCTCACTC TGGAGCTGTG GGCACAGGGC GTCCCGCCTG CTGTGGCCTC CCTGGACTTT 
GCCACTGAGC CTTACAATGC TGCCCGGCCC TACAGCGTGG CCCTGCTCAG TGTCCCCGAG 
GCCGCCCGGA CGGAAGCAGG GAAGCCAGAG AGCAGCACCC CCACAGGCGA GCCAGGCCCC 
ATGGCATCCA GCCCTGAGCC CGCTGTGGCC AAGGGAGGCT TCCTGAGCTT CCTTGAGGCC 
AACATGTTCA GCGTCATCAT CCCCATGTGC CTGGTACTTC TGCTCCTGGC GCTCATCCTG 
CCCCTGCTCT TCTACCTCCG AAAACGCAAC AAGACGGGCA AGCATGACGT CCAGGTCCTG 
ACTGCCAAGC CCCGCAACGG CCTGGCTGGT GACACCGAGA CCTTTCGCAA GGTGGAGCCA 
GGCCAGGCCA TCCCGCTCAC AGCTGTGCCT GGCCAGGGGC CCCCTCCAGG AGGCCAGCCT 
GACCCAGAGC TGCTGCAGTT CTGCCGGACA CCCAACCCTG CCCTTAAGAA TGGCCAGTAC 
TGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001888.2
CDS	94..7062
Translation	MQSGPRPPLPAPGLALALTLTMLARLASAASFFGENHLEVPVATALTDIDLQLQFSTSQPEALLLLAAGPADHLLLQLYSGRLQVRLVLGQEELRLQTPAETLLSDSIPHTVVLTVVEGWATLSVDGFLNASSAVPGAPLEVPYGLFVGGTGTLGLPYLRGTSRPLRGCLHAATLNGRSLLRPLTPDVHEGCAEEFSASDDVALGFSGPHSLAAFPAWGTQDEGTLEFTLTTQSRQAPLAFQAGGRRGDFIYVDIFEGHLRAVVEKGQGTVLLHNSVPVADGQPHEVSVHINAHRLEISVDQYPTHTSNRGVLSYLEPRGSLLLGGLDAEASRHLQEHRLGLTPEATNASLLGCMEDLSVNGQRRGLREALLTRNMAAGCRLEEEEYEDDAYGHYEAFSTLAPEAWPAMELPEPCVPEPGLPPVFANFTQLLTISPLVVAEGGTAWLEWRHVQPTLDLMEAELRKSQVLFSVTRGARHGELELDIPGAQARKMFTLLDVVNRKARFIHDGSEDTSDQLVLEVSVTARVPMPSCLRRGQTYLLPIQVNPVNDPPHIIFPHGSLMVILEHTQKPLGPEVFQAYDPDSACEGLTFQVLGTSSGLPVERRDQPGEPATEFSCRELEAGSLVYVHRGGPAQDLTFRVSDGLQASPPATLKVVAIRPAIQIHRSTGLRLAQGSAMPILPANLSVETNAVGQDVSVLFRVTGALQFGELQKQGAGGVEGAEWWATQAFHQRDVEQGRVRYLSTDPQHHAYDTVENLALEVQVGQEILSNLSFPVTIQRATVWMLRLEPLHTQNTQQETLTTAHLEATLEEAGPSPPTFHYEVVQAPRKGNLQLQGTRLSDGQGFTQDDIQAGRVTYGATARASEAVEDTFRFRVTAPPYFSPLYTFPIHIGGDPDAPVLTNVLLVVPEGGEGVLSADHLFVKSLNSASYLYEVMERPRHGRLAWRGTQDKTTMVTSFTNEDLLRGRLVYQHDDSETTEDDIPFVATRQGESSGDMAWEEVRGVFRVAIQPVNDHAPVQTISRIFHVARGGRRLLTTDDVAFSDADSGFADAQLVLTRKDLLFGSIVAVDEPTRPIYRFTQEDLRKRRVLFVHSGADRGWIQLQVSDGQHQATALLEVQASEPYLRVANGSSLVVPQGGQGTIDTAVLHLDTNLDIRSGDEVHYHVTAGPRWGQLVRAGQPATAFSQQDLLDGAVLYSHNGSLSPRDTMAFSVEAGPVHTDATLQVTIALEGPLAPLKLVRHKKIYVFQGEAAEIRRDQLEAAQEAVPPADIVFSVKSPPSAGYLVMVSRGALADEPPSLDPVQSFSQEAVDTGRVLYLHSRPEAWSDAFSLDVASGLGAPLEGVLVELEVLPAAIPLEAQNFSVPEGGSLTLAPPLLRVSGPYFPTLLGLSLQVLEPPQHGALQKEDGPQARTLSAFSWRMVEEQLIRYVHDGSETLTDSFVLMANASEMDRQSHPVAFTVTVLPVNDQPPILTTNTGLQMWEGATAPIPAEALRSTDGDSGSEDLVYTIEQPSNGRVVLRGAPGTEVRSFTQAQLDGGLVLFSHRGTLDGGFRFRLSDGEHTSPGHFFRVTAQKQVLLSLKGSQTLTVCPGSVQPLSSQTLRASSSAGTDPQLLLYRVVRGPQLGRLFHAQQDSTGEALVNFTQAEVYAGNILYEHEMPPEPFWEAHDTLELQLSSPPARDVAATLAVAVSFEAACPQRPSHLWKNKGLWVPEGQRARITVAALDASNLLASVPSPQRSEHDVLFQVTQFPSRGQLLVSEEPLHAGQPHFLQSQLAAGQLVYAHGGGGTQQDGFHFRAHLQGPAGASVAGPQTSEAFAITVRDVNERPPQPQASVPLRLTRGSRAPISRAQLSVVDPDSAPGEIEYEVQRAPHNGFLSLVGGGLGPVTRFTQADVDSGRLAFVANGSSVAGIFQLSMSDGASPPLPMSLAVDILPSAIEVQLRAPLEVPQALGRSSLSQQQLRVVSDREEPEAAYRLIQGPQYGHLLVGGRPTSAFSQFQIDQGEVVFAFTNFSSSHDHFRVLALARGVNASAVVNVTVRALLHVWAGGPWPQGATLRLDPTVLDAGELANRTGSVPRFRLLEGPRHGRVVRVPRARTEPGGSQLVEQFTQQDLEDGRLGLEVGRPEGRAPGPAGDSLTLELWAQGVPPAVASLDFATEPYNAARPYSVALLSVPEAARTEAGKPESSTPTGEPGPMASSPEPAVAKGGFLSFLEANMFSVIIPMCLVLLLLALILPLLFYLRKRNKTGKHDVQVLTAKPRNGLAGDTETFRKVEPGQAIPLTAVPGQGPPPGGQPDPELLQFCRTPNPALKNGQYWV

Target ORF information:

RefSeq Version	NM_001897.5
Organism	Homo sapiens(human)
Definition	Homo sapiens chondroitin sulfate proteoglycan 4 (CSPG4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001897.5

ORF Insert Sequence:

ATGCAGTCCG GGCCGCGGCC CCCACTTCCA GCCCCCGGCC TGGCCTTGGC TTTGACCCTG 
ACTATGTTGG CCAGACTTGC ATCCGCGGCT TCCTTCTTCG GTGAGAACCA CCTGGAGGTG 
CCTGTGGCCA CGGCTCTGAC CGACATAGAC CTGCAGCTGC AGTTCTCCAC GTCCCAGCCC 
GAAGCCCTCC TTCTCCTGGC AGCAGGCCCA GCTGACCACC TCCTGCTGCA GCTCTACTCT 
GGACGCCTGC AGGTCAGACT TGTTCTGGGC CAGGAGGAGC TGAGGCTGCA GACTCCAGCA 
GAGACGCTGC TGAGTGACTC CATCCCCCAC ACTGTGGTGC TGACTGTCGT AGAGGGCTGG 
GCCACGTTGT CAGTCGATGG GTTTCTGAAC GCCTCCTCAG CAGTCCCAGG AGCCCCCCTA 
GAGGTCCCCT ATGGGCTCTT TGTTGGGGGC ACTGGGACCC TTGGCCTGCC CTACCTGAGG 
GGAACCAGCC GACCCCTGAG GGGTTGCCTC CATGCAGCCA CCCTCAATGG CCGCAGCCTC 
CTCCGGCCTC TGACCCCCGA TGTGCATGAG GGCTGTGCTG AAGAGTTTTC TGCCAGTGAT 
GATGTGGCCC TGGGCTTCTC TGGGCCCCAC TCTCTGGCTG CCTTCCCTGC CTGGGGCACT 
CAGGACGAAG GAACCCTAGA GTTTACACTC ACCACACAGA GCCGGCAGGC ACCCTTGGCC 
TTCCAGGCAG GGGGCCGGCG TGGGGACTTC ATCTATGTGG ACATATTTGA GGGCCACCTG 
CGGGCCGTGG TGGAGAAGGG CCAGGGTACC GTATTGCTCC ACAACAGTGT GCCTGTGGCC 
GATGGGCAGC CCCATGAGGT CAGTGTCCAC ATCAATGCTC ACCGGCTGGA AATCTCCGTG 
GACCAGTACC CTACGCATAC TTCGAACCGA GGAGTCCTCA GCTACCTGGA GCCACGGGGC 
AGTCTCCTTC TCGGGGGGCT GGATGCAGAG GCCTCTCGTC ACCTCCAGGA ACACCGCCTG 
GGCCTGACAC CAGAGGCCAC CAATGCCTCC CTGCTGGGCT GCATGGAAGA CCTCAGTGTC 
AATGGCCAGA GGCGGGGGCT GCGGGAAGCT TTGCTGACGC GCAACATGGC AGCCGGCTGC 
AGGCTGGAGG AGGAGGAGTA TGAGGACGAT GCCTATGGAC ATTATGAAGC TTTCTCCACC 
CTGGCCCCTG AGGCTTGGCC AGCCATGGAG CTGCCTGAGC CATGCGTGCC TGAGCCAGGG 
CTGCCTCCTG TCTTTGCCAA TTTCACCCAG CTGCTGACTA TCAGCCCACT GGTGGTGGCC 
GAGGGGGGCA CAGCCTGGCT TGAGTGGAGG CATGTGCAGC CCACGCTGGA CCTGATGGAG 
GCTGAGCTGC GCAAATCCCA GGTGCTGTTC AGCGTGACCC GAGGGGCACG CCATGGCGAG 
CTCGAGCTGG ACATCCCGGG AGCCCAGGCA CGAAAAATGT TCACCCTCCT GGACGTGGTG 
AACCGCAAGG CCCGCTTCAT CCACGATGGC TCTGAGGACA CCTCCGACCA GCTGGTGCTG 
GAGGTGTCGG TGACGGCTCG GGTGCCCATG CCCTCATGCC TTCGGAGGGG CCAAACATAC 
CTCCTGCCCA TCCAGGTCAA CCCTGTCAAT GACCCACCCC ACATCATCTT CCCACATGGC 
AGCCTCATGG TGATCCTGGA ACACACGCAG AAGCCGCTGG GGCCTGAGGT TTTCCAGGCC 
TATGACCCGG ACTCTGCCTG TGAGGGCCTC ACCTTCCAGG TCCTTGGCAC CTCCTCTGGC 
CTCCCCGTGG AGCGCCGAGA CCAGCCTGGG GAGCCGGCGA CCGAGTTCTC CTGCCGGGAG 
TTGGAGGCCG GCAGCCTAGT CTATGTCCAC CGCGGTGGTC CTGCACAGGA CTTGACGTTC 
CGGGTCAGCG ATGGACTGCA GGCCAGCCCC CCGGCCACGC TGAAGGTGGT GGCCATCCGG 
CCGGCCATAC AGATCCACCG CAGCACAGGG TTGCGACTGG CCCAAGGCTC TGCCATGCCC 
ATCTTGCCCG CCAACCTGTC GGTGGAGACC AATGCCGTGG GGCAGGATGT GAGCGTGCTG 
TTCCGCGTCA CTGGGGCCCT GCAGTTTGGG GAGCTGCAGA AGCAGGGGGC AGGTGGGGTG 
GAGGGTGCTG AGTGGTGGGC CACACAGGCG TTCCACCAGC GGGATGTGGA GCAGGGCCGC 
GTGAGGTACC TGAGCACTGA CCCACAGCAC CACGCTTACG ACACCGTGGA GAACCTGGCC 
CTGGAGGTGC AGGTGGGCCA GGAGATCCTG AGCAATCTGT CCTTCCCAGT GACCATCCAG 
AGAGCCACTG TGTGGATGCT GCGGCTGGAG CCACTGCACA CTCAGAACAC CCAGCAGGAG 
ACCCTCACCA CAGCCCACCT GGAGGCCACC CTGGAGGAGG CAGGCCCAAG CCCCCCAACC 
TTCCATTATG AGGTGGTTCA GGCTCCCAGG AAAGGCAACC TTCAACTACA GGGCACAAGG 
CTGTCAGATG GCCAGGGCTT CACCCAGGAT GACATACAGG CTGGCCGGGT GACCTATGGG 
GCCACAGCAC GTGCCTCAGA GGCAGTCGAG GACACCTTCC GTTTCCGTGT CACAGCTCCA 
CCATATTTCT CCCCACTCTA TACCTTCCCC ATCCACATTG GTGGTGACCC AGATGCGCCT 
GTCCTCACCA ATGTCCTCCT CGTGGTGCCT GAGGGTGGTG AGGGTGTCCT CTCTGCTGAC 
CACCTCTTTG TCAAGAGTCT CAACAGTGCC AGCTACCTCT ATGAGGTCAT GGAGCGGCCC 
CGCCATGGGA GGTTGGCTTG GCGTGGGACA CAGGACAAGA CCACTATGGT GACATCCTTC 
ACCAATGAAG ACCTGTTGCG TGGCCGGCTG GTCTACCAGC ATGATGACTC CGAGACCACA 
GAAGATGATA TCCCATTTGT TGCTACCCGC CAGGGCGAGA GCAGTGGTGA CATGGCCTGG 
GAGGAGGTAC GGGGTGTCTT CCGAGTGGCC ATCCAGCCCG TGAATGACCA CGCCCCTGTG 
CAGACCATCA GCCGGATCTT CCATGTGGCC CGGGGTGGGC GGCGGCTGCT GACTACAGAC 
GACGTGGCCT TCAGCGATGC TGACTCGGGC TTTGCTGACG CCCAGCTGGT GCTTACCCGC 
AAGGACCTCC TCTTTGGCAG TATCGTGGCC GTAGATGAGC CCACGCGGCC CATCTACCGC 
TTCACCCAGG AGGACCTCAG GAAGAGGCGA GTACTGTTCG TGCACTCAGG GGCTGACCGT 
GGCTGGATCC AGCTGCAGGT GTCCGACGGG CAACACCAGG CCACTGCGCT GCTGGAGGTG 
CAGGCCTCGG AACCCTACCT CCGTGTGGCC AACGGCTCCA GCCTTGTGGT CCCTCAAGGA 
GGCCAGGGCA CCATCGACAC GGCCGTGCTC CACCTGGACA CCAACCTCGA CATCCGCAGT 
GGGGATGAGG TCCACTACCA CGTCACAGCT GGCCCTCGCT GGGGACAGCT AGTCCGGGCT 
GGTCAGCCAG CCACAGCCTT CTCCCAGCAG GACCTGCTGG ATGGGGCCGT TCTCTATAGC 
CACAATGGCA GCCTCAGCCC CCGCGACACC ATGGCCTTCT CCGTGGAAGC AGGGCCAGTG 
CACACGGATG CCACCCTACA AGTGACCATT GCCCTAGAGG GCCCACTGGC CCCACTGAAG 
CTGGTCCGGC ACAAGAAGAT CTACGTCTTC CAGGGAGAGG CAGCTGAGAT CAGAAGGGAC 
CAGCTGGAGG CAGCCCAGGA GGCAGTGCCA CCTGCAGACA TCGTATTCTC AGTGAAGAGC 
CCACCGAGTG CCGGCTACCT GGTGATGGTG TCGCGTGGCG CCTTGGCAGA TGAGCCACCC 
AGCCTGGACC CTGTGCAGAG CTTCTCCCAG GAGGCAGTGG ACACAGGCAG GGTCCTGTAC 
CTGCACTCCC GCCCTGAGGC CTGGAGCGAT GCCTTCTCGC TGGATGTGGC CTCAGGCCTG 
GGTGCTCCCC TCGAGGGCGT CCTTGTGGAG CTGGAGGTGC TGCCCGCTGC CATCCCACTA 
GAGGCGCAAA ACTTCAGCGT CCCTGAGGGT GGCAGCCTCA CCCTGGCCCC TCCACTGCTC 
CGTGTCTCCG GGCCCTACTT CCCCACTCTC CTGGGCCTCA GCCTGCAGGT GCTGGAGCCA 
CCCCAGCATG GAGCCCTGCA GAAGGAGGAC GGACCTCAAG CCAGGACCCT CAGCGCCTTC 
TCCTGGAGAA TGGTGGAAGA GCAGCTGATC CGCTACGTGC ATGACGGGAG CGAGACACTG 
ACAGACAGTT TTGTCCTGAT GGCTAATGCC TCCGAGATGG ATCGCCAGAG CCATCCTGTG 
GCCTTCACTG TCACTGTCCT GCCTGTCAAT GACCAACCCC CCATCCTCAC TACAAACACA 
GGCCTGCAGA TGTGGGAGGG GGCCACTGCG CCCATCCCTG CGGAGGCTCT GAGGAGCACG 
GACGGCGACT CTGGGTCTGA GGATCTGGTC TACACCATCG AGCAGCCCAG CAACGGGCGG 
GTAGTGCTGC GGGGGGCGCC GGGCACTGAG GTGCGCAGCT TCACGCAGGC CCAGCTGGAC 
GGCGGGCTCG TGCTGTTCTC ACACAGAGGA ACCCTGGATG GAGGCTTCCG CTTCCGCCTC 
TCTGACGGCG AGCACACTTC CCCCGGACAC TTCTTCCGAG TGACGGCCCA GAAGCAAGTG 
CTCCTCTCGC TGAAGGGCAG CCAGACACTG ACTGTCTGCC CAGGGTCCGT CCAGCCACTC 
AGCAGTCAGA CCCTCAGGGC CAGCTCCAGC GCAGGCACTG ACCCCCAGCT CCTGCTCTAC 
CGTGTGGTGC GGGGCCCCCA GCTAGGCCGG CTGTTCCACG CCCAGCAGGA CAGCACAGGG 
GAGGCCCTGG TGAACTTCAC TCAGGCAGAG GTCTACGCTG GGAATATTCT GTATGAGCAT 
GAGATGCCCC CCGAGCCCTT TTGGGAGGCC CATGATACCC TAGAGCTCCA GCTGTCCTCG 
CCGCCTGCCC GGGACGTGGC CGCCACCCTT GCTGTGGCTG TGTCTTTTGA GGCTGCCTGT 
CCCCAGCGCC CCAGCCACCT CTGGAAGAAC AAAGGTCTCT GGGTCCCCGA GGGCCAGCGG 
GCCAGGATCA CCGTGGCTGC TCTGGATGCC TCCAATCTCT TGGCCAGCGT TCCATCACCC 
CAGCGCTCAG AGCATGATGT GCTCTTCCAG GTCACACAGT TCCCCAGCCG GGGCCAGCTG 
TTGGTGTCCG AGGAGCCCCT CCATGCTGGG CAGCCCCACT TCCTGCAGTC CCAGCTGGCT 
GCAGGGCAGC TAGTGTATGC CCACGGCGGT GGGGGCACCC AGCAGGATGG CTTCCACTTT 
CGTGCCCACC TCCAGGGGCC AGCAGGGGCC TCCGTGGCTG GACCCCAAAC CTCAGAGGCC 
TTTGCCATCA CGGTGAGGGA TGTAAATGAG CGGCCCCCTC AGCCACAGGC CTCTGTCCCA 
CTCCGGCTCA CCCGAGGCTC TCGTGCCCCC ATCTCCCGGG CCCAGCTGAG TGTGGTGGAC 
CCAGACTCAG CTCCTGGGGA GATTGAGTAC GAGGTCCAGC GGGCACCCCA CAACGGCTTC 
CTCAGCCTGG TGGGTGGTGG CCTGGGGCCC GTGACCCGCT TCACGCAAGC CGATGTGGAT 
TCAGGGCGGC TGGCCTTCGT GGCCAACGGG AGCAGCGTGG CAGGCATCTT CCAGCTGAGC 
ATGTCTGATG GGGCCAGCCC ACCCCTGCCC ATGTCCCTGG CTGTGGACAT CCTACCATCC 
GCCATCGAGG TGCAGCTGCG GGCACCCCTG GAGGTGCCCC AAGCTTTGGG GCGCTCCTCA 
CTGAGCCAGC AGCAGCTCCG GGTGGTTTCA GATCGGGAGG AGCCAGAGGC AGCATACCGC 
CTCATCCAGG GACCCCAGTA TGGGCATCTC CTGGTGGGCG GGCGGCCCAC CTCGGCCTTC 
AGCCAATTCC AGATAGACCA GGGCGAGGTG GTCTTTGCCT TCACCAACTT CTCCTCCTCT 
CATGACCACT TCAGAGTCCT GGCACTGGCT AGGGGTGTCA ATGCATCAGC CGTAGTGAAC 
GTCACTGTGA GGGCTCTGCT GCATGTGTGG GCAGGTGGGC CATGGCCCCA GGGTGCCACC 
CTGCGCCTGG ACCCCACCGT CCTAGATGCT GGCGAGCTGG CCAACCGCAC AGGCAGTGTG 
CCGCGCTTCC GCCTCCTGGA GGGACCCCGG CATGGCCGCG TGGTCCGCGT GCCCCGAGCC 
AGGACGGAGC CCGGGGGCAG CCAGCTGGTG GAGCAGTTCA CTCAGCAGGA CCTTGAGGAC 
GGGAGGCTGG GGCTGGAGGT GGGCAGGCCA GAGGGGAGGG CCCCCGGCCC CGCAGGTGAC 
AGTCTCACTC TGGAGCTGTG GGCACAGGGC GTCCCGCCTG CTGTGGCCTC CCTGGACTTT 
GCCACTGAGC CTTACAATGC TGCCCGGCCC TACAGCGTGG CCCTGCTCAG TGTCCCCGAG 
GCCGCCCGGA CGGAAGCAGG GAAGCCAGAG AGCAGCACCC CCACAGGCGA GCCAGGCCCC 
ATGGCATCCA GCCCTGAGCC CGCTGTGGCC AAGGGAGGCT TCCTGAGCTT CCTTGAGGCC 
AACATGTTCA GCGTCATCAT CCCCATGTGC CTGGTACTTC TGCTCCTGGC GCTCATCCTG 
CCCCTGCTCT TCTACCTCCG AAAACGCAAC AAGACGGGCA AGCATGACGT CCAGGTCCTG 
ACTGCCAAGC CCCGCAACGG CCTGGCTGGT GACACCGAGA CCTTTCGCAA GGTGGAGCCA 
GGCCAGGCCA TCCCGCTCAC AGCTGTGCCT GGCCAGGGGC CCCCTCCAGG AGGCCAGCCT 
GACCCAGAGC TGCTGCAGTT CTGCCGGACA CCCAACCCTG CCCTTAAGAA TGGCCAGTAC 
TGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Methylation Status of Transcriptional Modulatory Genes Associated with Colorectal Cancer in Northeast China.
Gut and liver(2018 Jan)
Gao HL,Wang X,Sun HR,Zhou JD,Lin SQ,Xing YH,Zhu L,Zhou HB,Zhao YS,Chi Q,Liu YP

Effects of chondroitin sulfate proteoglycan 4 (NG2/CSPG4) on soft tissue sarcoma growth depend on tumor developmental stage.
The Journal of biological chemistry(2017 Dec)
Hsu SC,Nadesan P,Puviindran V,Stallcup WB,Kirsch DG,Alman BA

NG2/CSPG4 and progranulin in the posttraumatic glial scar.
Matrix biology : journal of the International Society for Matrix Biology(2017 Oct)
Sch?fer MKE,Tegeder I

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CSPG4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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