CRYGB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRYGB
Entrez Gene ID	1419
Full Name	crystallin gamma B
Synonyms	CRYG2,CTRCT39
General protein information	Preferred Names crystallin gamma B Names gamma-crystallin B crystallin, gamma 1-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q33.3
Summary	Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. [provided by RefSeq, Jul 2008].
Disorder MIM:	123670
Disorder Html:	Cataract 39, multiple types, autosomal dominant, 615188 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017003402.1	XP_016858891.1	gamma-crystallin B isoform X1
NM_005210.3	NP_005201.2	gamma-crystallin B
NM_005210.4	NP_005201.2	gamma-crystallin B

Pathways from BioSystems

Homologies

Homo sapiens (human)	CRYGB	NP_005201.2
Mus musculus (house mouse)	Crygb	NP_658906.1
Rattus norvegicus (Norway rat)	Crygb	NP_001103345.1
Canis lupus familiaris (dog)	CRYGB	NP_001104269.1
Danio rerio (zebrafish)	crygs3	NP_001012390.1
Pan troglodytes (chimpanzee)	CRYGB	NP_001129132.1
	CRYGB	NP_001076434.1
Bos taurus (cattle)	CRYGB	NP_001013612.1

Gene Ontology
Bibliography

Related articles in PubMed

Glycation of human ?B-crystallin: A biophysical investigation.
Chaudhury S, Ghosh P, Parveen S, Dasgupta S
International journal of biological macromolecules96392-402(2017 Mar)

[ALLELIC VARIANT FREQUENCY OF PROMOTER (G(-47)-->A) ?-CRYSTALLIN GENE AFFECTS THE LEVEL OF ITS EXPRESSION IN PLATELETS].
Rykov SA, Byts YY, Goncharov SV, Dosenko VE
Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)61(4)30-4(2015)

Novel crystallin gamma B mutations in a Kuwaiti family with autosomal dominant congenital cataracts reveal genetic and clinical heterogeneity.
AlFadhli S, Abdelmoaty S, Al-Hajeri A, Behbehani A, Alkuraya F
Molecular vision182931-6(2012)

Polymorphisms of the gamma crystallin A and B genes among Indian patients with pediatric cataract.
Mehra S, Kapur S, Vasavada AR
Journal of postgraduate medicine57(3)201-5(2011 Jul-Sep)

Partially folded aggregation intermediates of human gammaD-, gammaC-, and gammaS-crystallin are recognized and bound by human alphaB-crystallin chaperone.
Acosta-Sampson L, King J
Journal of molecular biology401(1)134-52(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Glycation of human gammaB-crystallin
Title: Glycation of human ?B-crystallin: A biophysical investigation.

Allelic variant frequency of the gamma-crystallin promoter (g(-47) ->a) affects the level of its expression in platelets from cataract patients.
Title: [ALLELIC VARIANT FREQUENCY OF PROMOTER (G(-47)-->A) ?-CRYSTALLIN GENE AFFECTS THE LEVEL OF ITS EXPRESSION IN PLATELETS].

Complex heterogeneous mutations in the gammaB crystallin gene have been described resulting in autosomal dominant congenital cataracts with three distinct phenotypes (lamellar, anterior polar, and complete cataracts) in the same family.
Title: Novel crystallin gamma B mutations in a Kuwaiti family with autosomal dominant congenital cataracts reveal genetic and clinical heterogeneity.

-47C allele of rs2289917 in CRYGB showed the strongest association with cataract.
Title: Polymorphisms of the gamma crystallin A and B genes among Indian patients with pediatric cataract.

Observational study of gene-disease association. (HuGE Navigator)
Title: Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.

The following CRYGB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRYGB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19107	NM_005210.4 Latest version!	Homo sapiens crystallin gamma B (CRYGB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu89044	XM_017003402.1 Latest version!	Homo sapiens crystallin gamma B (CRYGB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu19107	NM_005210.3	Homo sapiens crystallin gamma B (CRYGB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRYGB ( NM_005210.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005201.2 Homo sapiens crystallin gamma B (CRYGB), mRNA.

Price & Availability↑

CloneID	OHu19107
Clone ID Related Accession (Same CDS sequence)	NM_005210.3 , NM_005210.4
Accession Version	NM_005210.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-26
Organism	Homo sapiens(human)
Product	gamma-crystallin B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY794984.2 and BM666147.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2007 this sequence version replaced NM_005210.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC117384.1, KT326704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAAAGA TCACCTTCTA CGAGGACAGG GCCTTCCAGG GCCGCAGCTA CGAATGCACC 
ACTGACTGCC CCAACCTACA ACCCTATTTC AGCCGCTGCA ACTCCATCAG GGTGGAGAGC 
GGCTGCTGGA TGATCTATGA GCGCCCCAAC TACCAGGGCC ACCAGTACTT CCTGCGGCGT 
GGGGAGTACC CTGACTACCA GCAATGGATG GGCCTCAGCG ACTCCATCCG CTCCTGCTGC 
CTCATCCCCC CGCACTCTGG CGCTTACAGA ATGAAGATCT ACGACAGAGA TGAATTGAGG 
GGACAAATGT CAGAGCTCAC AGACGACTGT ATCTCTGTTC AGGACCGCTT CCACCTCACT 
GAAATTCACT CCCTCAATGT GCTGGAGGGC AGCTGGATCC TCTATGAGAT GCCCAACTAC 
AGGGGGAGGC AGTATCTGCT GAGGCCGGGG GAGTACAGGA GGTTTCTTGA TTGGGGGGCT 
CCAAATGCCA AAGTTGGCTC TCTTAGACGA GTCATGGATT TGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005201.2
CDS	34..561
Translation	MGKITFYEDRAFQGRSYECTTDCPNLQPYFSRCNSIRVESGCWMIYERPNYQGHQYFLRRGEYPDYQQWMGLSDSIRSCCLIPPHSGAYRMKIYDRDELRGQMSELTDDCISVQDRFHLTEIHSLNVLEGSWILYEMPNYRGRQYLLRPGEYRRFLDWGAPNAKVGSLRRVMDLY

Target ORF information:

RefSeq Version	NM_005210.3
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin gamma B (CRYGB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005210.3

ORF Insert Sequence:

ATGGGAAAGA TCACCTTCTA CGAGGACAGG GCCTTCCAGG GCCGCAGCTA CGAATGCACC 
ACTGACTGCC CCAACCTACA ACCCTATTTC AGCCGCTGCA ACTCCATCAG GGTGGAGAGC 
GGCTGCTGGA TGATCTATGA GCGCCCCAAC TACCAGGGCC ACCAGTACTT CCTGCGGCGT 
GGGGAGTACC CTGACTACCA GCAATGGATG GGCCTCAGCG ACTCCATCCG CTCCTGCTGC 
CTCATCCCCC CGCACTCTGG CGCTTACAGA ATGAAGATCT ACGACAGAGA TGAATTGAGG 
GGACAAATGT CAGAGCTCAC AGACGACTGT ATCTCTGTTC AGGACCGCTT CCACCTCACT 
GAAATTCACT CCCTCAATGT GCTGGAGGGC AGCTGGATCC TCTATGAGAT GCCCAACTAC 
AGGGGGAGGC AGTATCTGCT GAGGCCGGGG GAGTACAGGA GGTTTCTTGA TTGGGGGGCT 
CCAAATGCCA AAGTTGGCTC TCTTAGACGA GTCATGGATT TGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYGB ( NM_005210.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005201.2 Homo sapiens crystallin gamma B (CRYGB), mRNA.

Price & Availability↑

CloneID	OHu19107
Clone ID Related Accession (Same CDS sequence)	NM_005210.3 , NM_005210.4
Accession Version	NM_005210.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	gamma-crystallin B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC016697.8, BY794984.2 and BM666147.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005210.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC117384.1, KT326704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000260988.5/ ENSP00000260988.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGAAAGA TCACCTTCTA CGAGGACAGG GCCTTCCAGG GCCGCAGCTA CGAATGCACC 
ACTGACTGCC CCAACCTACA ACCCTATTTC AGCCGCTGCA ACTCCATCAG GGTGGAGAGC 
GGCTGCTGGA TGATCTATGA GCGCCCCAAC TACCAGGGCC ACCAGTACTT CCTGCGGCGT 
GGGGAGTACC CTGACTACCA GCAATGGATG GGCCTCAGCG ACTCCATCCG CTCCTGCTGC 
CTCATCCCCC CGCACTCTGG CGCTTACAGA ATGAAGATCT ACGACAGAGA TGAATTGAGG 
GGACAAATGT CAGAGCTCAC AGACGACTGT ATCTCTGTTC AGGACCGCTT CCACCTCACT 
GAAATTCACT CCCTCAATGT GCTGGAGGGC AGCTGGATCC TCTATGAGAT GCCCAACTAC 
AGGGGGAGGC AGTATCTGCT GAGGCCGGGG GAGTACAGGA GGTTTCTTGA TTGGGGGGCT 
CCAAATGCCA AAGTTGGCTC TCTTAGACGA GTCATGGATT TGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005201.2
CDS	39..566
Translation	MGKITFYEDRAFQGRSYECTTDCPNLQPYFSRCNSIRVESGCWMIYERPNYQGHQYFLRRGEYPDYQQWMGLSDSIRSCCLIPPHSGAYRMKIYDRDELRGQMSELTDDCISVQDRFHLTEIHSLNVLEGSWILYEMPNYRGRQYLLRPGEYRRFLDWGAPNAKVGSLRRVMDLY

Target ORF information:

RefSeq Version	NM_005210.4
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin gamma B (CRYGB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005210.4

ORF Insert Sequence:

ATGGGAAAGA TCACCTTCTA CGAGGACAGG GCCTTCCAGG GCCGCAGCTA CGAATGCACC 
ACTGACTGCC CCAACCTACA ACCCTATTTC AGCCGCTGCA ACTCCATCAG GGTGGAGAGC 
GGCTGCTGGA TGATCTATGA GCGCCCCAAC TACCAGGGCC ACCAGTACTT CCTGCGGCGT 
GGGGAGTACC CTGACTACCA GCAATGGATG GGCCTCAGCG ACTCCATCCG CTCCTGCTGC 
CTCATCCCCC CGCACTCTGG CGCTTACAGA ATGAAGATCT ACGACAGAGA TGAATTGAGG 
GGACAAATGT CAGAGCTCAC AGACGACTGT ATCTCTGTTC AGGACCGCTT CCACCTCACT 
GAAATTCACT CCCTCAATGT GCTGGAGGGC AGCTGGATCC TCTATGAGAT GCCCAACTAC 
AGGGGGAGGC AGTATCTGCT GAGGCCGGGG GAGTACAGGA GGTTTCTTGA TTGGGGGGCT 
CCAAATGCCA AAGTTGGCTC TCTTAGACGA GTCATGGATT TGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYGB ( XM_017003402.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858891.1 Homo sapiens crystallin gamma B (CRYGB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu89044
Clone ID Related Accession (Same CDS sequence)	XM_017003402.1
Accession Version	XM_017003402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	gamma-crystallin B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAGA TCACCTTCTA CGAGGACAGG GCCTTCCAGG GCCGCAGCTA CGAATGCACC 
ACTGACTGCC CCAACCTACA ACCCTATTTC AGCCGCTGCA ACTCCATCAG GGTGGAGAGC 
GGCTGCTGGA TGATCTATGA GCGCCCCAAC TACCAGGGCC ACCAGTACTT CCTGCGGCGT 
GGGGAGTACC CTGACTACCA GCAATGGATG GGCCTCAGCG ACTCCATCCG CTCCTGCTGC 
CTCATCCCCC CGGTGAGTCA CTCTGGCGCT TACAGAATGA AGATCTACGA CAGAGATGAA 
TTGAGGGGAC AAATGTCAGA GCTCACAGAC GACTGTATCT CTGTTCAGGA CCGCTTCCAC 
CTCACTGAAA TTCACTCCCT CAATGTGCTG GAGGGCAGCT GGATCCTCTA TGAGATGCCC 
AACTACAGGG GGAGGCAGTA TCTGCTGAGG CCGGGGGAGT ACAGGAGGTT TCTTGATTGG 
GGGGCTCCAA ATGCCAAAGT TGGCTCTCTT AGACGAGTCA TGGATTTGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858891.1
CDS	208..741
Translation	MGKITFYEDRAFQGRSYECTTDCPNLQPYFSRCNSIRVESGCWMIYERPNYQGHQYFLRRGEYPDYQQWMGLSDSIRSCCLIPPVSHSGAYRMKIYDRDELRGQMSELTDDCISVQDRFHLTEIHSLNVLEGSWILYEMPNYRGRQYLLRPGEYRRFLDWGAPNAKVGSLRRVMDLY

Target ORF information:

RefSeq Version	XM_017003402.1
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin gamma B (CRYGB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003402.1

ORF Insert Sequence:

ATGGGAAAGA TCACCTTCTA CGAGGACAGG GCCTTCCAGG GCCGCAGCTA CGAATGCACC 
ACTGACTGCC CCAACCTACA ACCCTATTTC AGCCGCTGCA ACTCCATCAG GGTGGAGAGC 
GGCTGCTGGA TGATCTATGA GCGCCCCAAC TACCAGGGCC ACCAGTACTT CCTGCGGCGT 
GGGGAGTACC CTGACTACCA GCAATGGATG GGCCTCAGCG ACTCCATCCG CTCCTGCTGC 
CTCATCCCCC CGGTGAGTCA CTCTGGCGCT TACAGAATGA AGATCTACGA CAGAGATGAA 
TTGAGGGGAC AAATGTCAGA GCTCACAGAC GACTGTATCT CTGTTCAGGA CCGCTTCCAC 
CTCACTGAAA TTCACTCCCT CAATGTGCTG GAGGGCAGCT GGATCCTCTA TGAGATGCCC 
AACTACAGGG GGAGGCAGTA TCTGCTGAGG CCGGGGGAGT ACAGGAGGTT TCTTGATTGG 
GGGGCTCCAA ATGCCAAAGT TGGCTCTCTT AGACGAGTCA TGGATTTGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycation of human ?B-crystallin: A biophysical investigation.
International journal of biological macromolecules96392-402(2017 Mar)
Chaudhury S,Ghosh P,Parveen S,Dasgupta S

[ALLELIC VARIANT FREQUENCY OF PROMOTER (G(-47)-->A) ?-CRYSTALLIN GENE AFFECTS THE LEVEL OF ITS EXPRESSION IN PLATELETS].
Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)61(4)30-4(2015)
Rykov SA,Byts YY,Goncharov SV,Dosenko VE

Novel crystallin gamma B mutations in a Kuwaiti family with autosomal dominant congenital cataracts reveal genetic and clinical heterogeneity.
Molecular vision182931-6(2012)
AlFadhli S,Abdelmoaty S,Al-Hajeri A,Behbehani A,Alkuraya F

Polymorphisms of the gamma crystallin A and B genes among Indian patients with pediatric cataract.
Journal of postgraduate medicine57(3)201-5(2011 Jul-Sep)
Mehra S,Kapur S,Vasavada AR

Partially folded aggregation intermediates of human gammaD-, gammaC-, and gammaS-crystallin are recognized and bound by human alphaB-crystallin chaperone.
Journal of molecular biology401(1)134-52(2010 Aug)
Acosta-Sampson L,King J

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CRYGB cDNA ORF clone, Homo sapiens(human)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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