CRYGA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRYGA
Entrez Gene ID	1418
Full Name	crystallin gamma A
Synonyms	CRY-g-A,CRYG1,CRYG5
General protein information	Preferred Names crystallin gamma A Names gamma-crystallin A crystallin, gamma 1 gamma-crystallin 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q33.3
Summary	Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. [provided by RefSeq, Jul 2008].
Disorder MIM:	123660

mRNA and Protein(s)

mRNA	Protein	Name
NM_014617.3	NP_055432.2	gamma-crystallin A
NM_014617.4	NP_055432.2	gamma-crystallin A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	crygmx	NP_001013280.1
Pan troglodytes (chimpanzee)	CRYGA	NP_001094072.1
Canis lupus familiaris (dog)	CRYGA	NP_001106641.1
Mus musculus (house mouse)	Cryga	NP_031800.1
	CRYGA	NP_001094290.1
Bos taurus (cattle)	CRYGA	NP_001230499.1
Homo sapiens (human)	CRYGA	NP_055432.2
Rattus norvegicus (Norway rat)	Cryga	NP_001074405.1

Gene Ontology
Bibliography

Related articles in PubMed

Lens aging: effects of crystallins.
Sharma KK, Santhoshkumar P
Biochimica et biophysica acta1790(10)1095-108(2009 Oct)

Analysis of single nucleotide polymorphisms of CRYGA and CRYGB genes in control population of western Indian origin.
Kapur S, Mehra S, Gajjar D, Vasavada A, Kapoor M, Sharad S, Alapure B, Rajkumar S
Indian journal of ophthalmology57(3)197-201(2009 May-Jun)

Homology models of human gamma-crystallins: structural study of the extensive charge network in gamma-crystallins.
Salim A, Zaidi ZH
Biochemical and biophysical research communications300(3)624-30(2003 Jan)

Novel mutations in the gamma-crystallin genes cause autosomal dominant congenital cataracts.
Santhiya ST, Shyam Manohar M, Rawlley D, Vijayalakshmi P, Namperumalsamy P, Gopinath PM, L?ster J, Graw J
Journal of medical genetics39(5)352-8(2002 May)

Structure of the crystallins.
Slingsby C, Clout NJ
Eye (London, England)13 ( Pt 3b)395-402(1999 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies show that The major proteins in the lens--alpha, beta, and gamma-crystallins--are constantly subjected to age-related changes.
Title: Lens aging: effects of crystallins.

This study establishes baseline frequency data for four SNPs in CRYGA and CRYGB genes for future case control studies on the role of these SNPs in the genetic basis of cataract.
Title: Analysis of single nucleotide polymorphisms of CRYGA and CRYGB genes in control population of western Indian origin.

The following CRYGA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRYGA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19721	NM_014617.4 Latest version!	Homo sapiens crystallin gamma A (CRYGA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu19721	NM_014617.3	Homo sapiens crystallin gamma A (CRYGA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRYGA ( NM_014617.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055432.2 Homo sapiens crystallin gamma A (CRYGA), mRNA.

Price & Availability↑

CloneID	OHu19721
Clone ID Related Accession (Same CDS sequence)	NM_014617.3 , NM_014617.4
Accession Version	NM_014617.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	gamma-crystallin A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM698524.1 and EF426311.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2007 this sequence version replaced NM_014617.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU737436.1, KT001516.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148093, SAMN03465403 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGA TCACCTTCTA CGAGGACCGA GACTTTCAGG GTCGCTGCTA CAATTGCATC 
AGTGACTGCC CCAACCTGCG GGTCTACTTC AGCCGCTGCA ACTCCATCCG AGTAGACAGC 
GGCTGCTGGA TGCTCTATGA GCGTCCCAAT TACCAGGGCC ACCAGTACTT CCTGCGCCGA 
GGCAAGTACC CCGACTATCA GCACTGGATG GGCCTCAGCG ACTCGGTCCA ATCCTGCCGT 
ATAATTCCTC ATACCAGCTC GCACAAGTTA AGGCTGTACG AGAGAGATGA CTACCGAGGC 
CTTATGTCTG AGCTCACTGA TGACTGCGCC TGTGTTCCAG AACTGTTCCG TCTCCCTGAG 
ATCTATTCCC TCCACGTACT GGAGGGCTGC TGGGTCCTCT ATGAAATGCC CAACTACCGG 
GGGCGGCAGT ATCTGCTGAG GCCTGGGGAC TACAGAAGGT ACCACGACTG GGGGGGTGCA 
GATGCCAAAG TCGGCTCTTT GAGACGGGTC ACCGATTTGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055432.2
CDS	18..542
Translation	MGKITFYEDRDFQGRCYNCISDCPNLRVYFSRCNSIRVDSGCWMLYERPNYQGHQYFLRRGKYPDYQHWMGLSDSVQSCRIIPHTSSHKLRLYERDDYRGLMSELTDDCACVPELFRLPEIYSLHVLEGCWVLYEMPNYRGRQYLLRPGDYRRYHDWGGADAKVGSLRRVTDLY

Target ORF information:

RefSeq Version	NM_014617.3
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin gamma A (CRYGA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014617.3

ORF Insert Sequence:

ATGGGGAAGA TCACCTTCTA CGAGGACCGA GACTTTCAGG GTCGCTGCTA CAATTGCATC 
AGTGACTGCC CCAACCTGCG GGTCTACTTC AGCCGCTGCA ACTCCATCCG AGTAGACAGC 
GGCTGCTGGA TGCTCTATGA GCGTCCCAAT TACCAGGGCC ACCAGTACTT CCTGCGCCGA 
GGCAAGTACC CCGACTATCA GCACTGGATG GGCCTCAGCG ACTCGGTCCA ATCCTGCCGT 
ATAATTCCTC ATACCAGCTC GCACAAGTTA AGGCTGTACG AGAGAGATGA CTACCGAGGC 
CTTATGTCTG AGCTCACTGA TGACTGCGCC TGTGTTCCAG AACTGTTCCG TCTCCCTGAG 
ATCTATTCCC TCCACGTACT GGAGGGCTGC TGGGTCCTCT ATGAAATGCC CAACTACCGG 
GGGCGGCAGT ATCTGCTGAG GCCTGGGGAC TACAGAAGGT ACCACGACTG GGGGGGTGCA 
GATGCCAAAG TCGGCTCTTT GAGACGGGTC ACCGATTTGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYGA ( NM_014617.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055432.2 Homo sapiens crystallin gamma A (CRYGA), mRNA.

Price & Availability↑

CloneID	OHu19721
Clone ID Related Accession (Same CDS sequence)	NM_014617.3 , NM_014617.4
Accession Version	NM_014617.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	gamma-crystallin A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC016697.8, BM698524.1 and EF426311.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_014617.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU737436.1, EF426311.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148093, SAMN03465403 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000304502.5/ ENSP00000302105.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGA TCACCTTCTA CGAGGACCGA GACTTTCAGG GTCGCTGCTA CAATTGCATC 
AGTGACTGCC CCAACCTGCG GGTCTACTTC AGCCGCTGCA ACTCCATCCG AGTAGACAGC 
GGCTGCTGGA TGCTCTATGA GCGTCCCAAT TACCAGGGCC ACCAGTACTT CCTGCGCCGA 
GGCAAGTACC CCGACTATCA GCACTGGATG GGCCTCAGCG ACTCGGTCCA ATCCTGCCGT 
ATAATTCCTC ATACCAGCTC GCACAAGTTA AGGCTGTACG AGAGAGATGA CTACCGAGGC 
CTTATGTCTG AGCTCACTGA TGACTGCGCC TGTGTTCCAG AACTGTTCCG TCTCCCTGAG 
ATCTATTCCC TCCACGTACT GGAGGGCTGC TGGGTCCTCT ATGAAATGCC CAACTACCGG 
GGGCGGCAGT ATCTGCTGAG GCCTGGGGAC TACAGAAGGT ACCACGACTG GGGGGGTGCA 
GATGCCAAAG TCGGCTCTTT GAGACGGGTC ACCGATTTGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055432.2
CDS	34..558
Translation	MGKITFYEDRDFQGRCYNCISDCPNLRVYFSRCNSIRVDSGCWMLYERPNYQGHQYFLRRGKYPDYQHWMGLSDSVQSCRIIPHTSSHKLRLYERDDYRGLMSELTDDCACVPELFRLPEIYSLHVLEGCWVLYEMPNYRGRQYLLRPGDYRRYHDWGGADAKVGSLRRVTDLY

Target ORF information:

RefSeq Version	NM_014617.4
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin gamma A (CRYGA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014617.4

ORF Insert Sequence:

ATGGGGAAGA TCACCTTCTA CGAGGACCGA GACTTTCAGG GTCGCTGCTA CAATTGCATC 
AGTGACTGCC CCAACCTGCG GGTCTACTTC AGCCGCTGCA ACTCCATCCG AGTAGACAGC 
GGCTGCTGGA TGCTCTATGA GCGTCCCAAT TACCAGGGCC ACCAGTACTT CCTGCGCCGA 
GGCAAGTACC CCGACTATCA GCACTGGATG GGCCTCAGCG ACTCGGTCCA ATCCTGCCGT 
ATAATTCCTC ATACCAGCTC GCACAAGTTA AGGCTGTACG AGAGAGATGA CTACCGAGGC 
CTTATGTCTG AGCTCACTGA TGACTGCGCC TGTGTTCCAG AACTGTTCCG TCTCCCTGAG 
ATCTATTCCC TCCACGTACT GGAGGGCTGC TGGGTCCTCT ATGAAATGCC CAACTACCGG 
GGGCGGCAGT ATCTGCTGAG GCCTGGGGAC TACAGAAGGT ACCACGACTG GGGGGGTGCA 
GATGCCAAAG TCGGCTCTTT GAGACGGGTC ACCGATTTGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lens aging: effects of crystallins.
Biochimica et biophysica acta1790(10)1095-108(2009 Oct)
Sharma KK,Santhoshkumar P

Analysis of single nucleotide polymorphisms of CRYGA and CRYGB genes in control population of western Indian origin.
Indian journal of ophthalmology57(3)197-201(2009 May-Jun)
Kapur S,Mehra S,Gajjar D,Vasavada A,Kapoor M,Sharad S,Alapure B,Rajkumar S

Homology models of human gamma-crystallins: structural study of the extensive charge network in gamma-crystallins.
Biochemical and biophysical research communications300(3)624-30(2003 Jan)
Salim A,Zaidi ZH

Novel mutations in the gamma-crystallin genes cause autosomal dominant congenital cataracts.
Journal of medical genetics39(5)352-8(2002 May)
Santhiya ST,Shyam Manohar M,Rawlley D,Vijayalakshmi P,Namperumalsamy P,Gopinath PM,L?ster J,Graw J

Structure of the crystallins.
Eye (London, England)13 ( Pt 3b)395-402(1999 Jun)
Slingsby C,Clout NJ

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CRYGA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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