CRYBA4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRYBA4
Entrez Gene ID	1413
Full Name	crystallin beta A4
Synonyms	CTRCT23,CYRBA4,MCOPCT4
General protein information	Preferred Names crystallin beta A4 Names beta-crystallin A4 beta crystallin A4 chain transcript PS beta crystallin alpha 4 chain beta-A4 crystallin crystallin, beta polypeptide A4 eye lens structural protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q12.1
Summary	Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, is part of a gene cluster with beta-B1, beta-B2, and beta-B3. [provided by RefSeq, Jul 2008].
Disorder MIM:	123631
Disorder Html:	Cataract 23, 610425 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001886.3	NP_001877.1	beta-crystallin A4
NM_001886.2	NP_001877.1	beta-crystallin A4
XM_006724140.3	XP_006724203.1	beta-crystallin A4 isoform X2
XM_017028598.1	XP_016884087.1	beta-crystallin A4 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cryba4	NP_113877.1
Homo sapiens (human)	CRYBA4	NP_001877.1
Xenopus tropicalis (tropical clawed frog)	cryba4	NP_001096487.1
Pan troglodytes (chimpanzee)	CRYBA4	XP_525555.2
Mus musculus (house mouse)	Cryba4	NP_067326.1
Danio rerio (zebrafish)	cryba4	NP_001018135.1
	CRYBA4	NP_001159483.1
Canis lupus familiaris (dog)	CRYBA4	NP_001074366.1
Gallus gallus (chicken)	CRYBA4	NP_989509.1
Bos taurus (cattle)	CRYBA4	NP_776950.1

Gene Ontology
Bibliography

Related articles in PubMed

Partial duplication of the CRYBB1-CRYBA4 locus is associated with autosomal dominant congenital cataract.
Siggs OM, Javadiyan S, Sharma S, Souzeau E, Lower KM, Taranath DA, Black J, Pater J, Willoughby JG, Burdon KP, Craig JE
European journal of human genetics : EJHG25(6)711-718(2017 06)

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Association of high myopia with crystallin beta A4 (CRYBA4) gene polymorphisms in the linkage-identified MYP6 locus.
Ho DW, Yap MK, Ng PW, Fung WY, Yip SP
PloS one7(6)e40238(2012)

A genome-wide meta-analysis identifies novel loci associated with schizophrenia and bipolar disorder.
Wang KS, Liu XF, Aragam N
Schizophrenia research124(1-3)192-9(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CRYBA4 duplication and CRYBB1 partial duplication identified in a family with autosomal dominant congenital cataract.
Title: Partial duplication of the CRYBB1-CRYBA4 locus is associated with autosomal dominant congenital cataract.

rs2009066 located in the crystallin beta A4 (CRYBA4) gene was identified to be the most significantly associated with high myopia.
Title: Association of high myopia with crystallin beta A4 (CRYBA4) gene polymorphisms in the linkage-identified MYP6 locus.

Novel missense mutation in CRYBA4 expands mutation spectrum of CRYBA4 and provides useful information to study of molecular pathogenesis of cataract and microcornea.
Title: A missense mutation in CRYBA4 associated with congenital cataract and microcornea.

Observational study of gene-disease association. (HuGE Navigator)
Title: Mutational screening of 10 genes in Chinese patients with microphthalmia and/or coloboma.

first report linking mutations in CRYBA4 to cataractogenesis and microphthalmia
Title: CRYBA4, a novel human cataract gene, is also involved in microphthalmia.

The following CRYBA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRYBA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26015	NM_001886.3 Latest version!	Homo sapiens crystallin beta A4 (CRYBA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu53412	XM_006724140.3 Latest version!	Homo sapiens crystallin beta A4 (CRYBA4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu93369	XM_017028598.1 Latest version!	Homo sapiens crystallin beta A4 (CRYBA4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu26015	NM_001886.2	Homo sapiens crystallin beta A4 (CRYBA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRYBA4 ( NM_001886.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001877.1 Homo sapiens crystallin beta A4 (CRYBA4), mRNA.

Price & Availability↑

CloneID	OHu26015
Clone ID Related Accession (Same CDS sequence)	NM_001886.3 , NM_001886.2
Accession Version	NM_001886.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	beta-crystallin A4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z95115.1, BC069404.1, BF726612.1, BC096172.3 and BM667295.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001886.2. Sequence Note: Experimental evidence for the bovine betaA4-crystallin in PMID: 1874445 suggests that a possible translation start codon would exist at nt 3-5 in this human sequence. No experimental evidence currently exists for a longer protein being produced in human cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U59057.1, KM365089.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:8999933) MANE Ensembl match :: ENST00000354760.4/ ENSP00000346805.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCCTGC AATGCACAAA GTCAGCGGGA CCCTGGAAGA TGGTGGTGTG GGATGAGGAC 
GGCTTCCAGG GCCGGCGGCA CGAGTTCACG GCCGAGTGCC CCAGCGTGCT GGAGCTTGGC 
TTCGAGACTG TGCGATCTTT GAAAGTGCTG AGTGGAGCGT GGGTGGGCTT TGAGCATGCT 
GGCTTCCAAG GGCAGCAGTA CATTCTGGAA CGAGGCGAAT ATCCAAGCTG GGATGCCTGG 
GGCGGCAACA CGGCCTACCC CGCCGAGAGG CTCACCTCCT TCCGGCCTGC GGCCTGTGCT 
AACCACCGTG ACTCGAGGCT GACAATCTTC GAGCAAGAGA ACTTCCTGGG CAAGAAAGGA 
GAGCTGAGCG ATGACTATCC TTCCCTCCAG GCCATGGGAT GGGAAGGCAA TGAAGTAGGG 
TCCTTCCACG TCCACTCTGG GGCCTGGGTT TGCTCCCAGT TTCCGGGCTA CCGAGGATTT 
CAGTATGTGC TGGAATGCGA TCACCATTCC GGTGACTACA AACATTTCCG GGAGTGGGGC 
TCTCATGCCC CGACCTTCCA GGTGCAGAGC ATCCGCAGGA TCCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001877.1
CDS	37..627
Translation	MTLQCTKSAGPWKMVVWDEDGFQGRRHEFTAECPSVLELGFETVRSLKVLSGAWVGFEHAGFQGQQYILERGEYPSWDAWGGNTAYPAERLTSFRPAACANHRDSRLTIFEQENFLGKKGELSDDYPSLQAMGWEGNEVGSFHVHSGAWVCSQFPGYRGFQYVLECDHHSGDYKHFREWGSHAPTFQVQSIRRIQQ

Target ORF information:

RefSeq Version	NM_001886.3
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin beta A4 (CRYBA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001886.3

ORF Insert Sequence:

ATGACCCTGC AATGCACAAA GTCAGCGGGA CCCTGGAAGA TGGTGGTGTG GGATGAGGAC 
GGCTTCCAGG GCCGGCGGCA CGAGTTCACG GCCGAGTGCC CCAGCGTGCT GGAGCTTGGC 
TTCGAGACTG TGCGATCTTT GAAAGTGCTG AGTGGAGCGT GGGTGGGCTT TGAGCATGCT 
GGCTTCCAAG GGCAGCAGTA CATTCTGGAA CGAGGCGAAT ATCCAAGCTG GGATGCCTGG 
GGCGGCAACA CGGCCTACCC CGCCGAGAGG CTCACCTCCT TCCGGCCTGC GGCCTGTGCT 
AACCACCGTG ACTCGAGGCT GACAATCTTC GAGCAAGAGA ACTTCCTGGG CAAGAAAGGA 
GAGCTGAGCG ATGACTATCC TTCCCTCCAG GCCATGGGAT GGGAAGGCAA TGAAGTAGGG 
TCCTTCCACG TCCACTCTGG GGCCTGGGTT TGCTCCCAGT TTCCGGGCTA CCGAGGATTT 
CAGTATGTGC TGGAATGCGA TCACCATTCC GGTGACTACA AACATTTCCG GGAGTGGGGC 
TCTCATGCCC CGACCTTCCA GGTGCAGAGC ATCCGCAGGA TCCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBA4 ( NM_001886.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001877.1 Homo sapiens crystallin beta A4 (CRYBA4), mRNA.

Price & Availability↑

CloneID	OHu26015
Clone ID Related Accession (Same CDS sequence)	NM_001886.3 , NM_001886.2
Accession Version	NM_001886.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	beta-crystallin A4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC069404.1, BF726612.1, BC096172.3 and BM667295.1. This sequence is a reference standard in the RefSeqGene project. On Sep 6, 2007 this sequence version replaced NM_001886.1. Sequence Note: Experimental evidence for the bovine betaA4-crystallin in PMID: 1874445 suggests that a possible translation start codon would exist at nt 3-5 in this human sequence. No experimental evidence currently exists for a longer protein being produced in human cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U59057.1, KM365088.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:8999933) ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCCTGC AATGCACAAA GTCAGCGGGA CCCTGGAAGA TGGTGGTGTG GGATGAGGAC 
GGCTTCCAGG GCCGGCGGCA CGAGTTCACG GCCGAGTGCC CCAGCGTGCT GGAGCTTGGC 
TTCGAGACTG TGCGATCTTT GAAAGTGCTG AGTGGAGCGT GGGTGGGCTT TGAGCATGCT 
GGCTTCCAAG GGCAGCAGTA CATTCTGGAA CGAGGCGAAT ATCCAAGCTG GGATGCCTGG 
GGCGGCAACA CGGCCTACCC CGCCGAGAGG CTCACCTCCT TCCGGCCTGC GGCCTGTGCT 
AACCACCGTG ACTCGAGGCT GACAATCTTC GAGCAAGAGA ACTTCCTGGG CAAGAAAGGA 
GAGCTGAGCG ATGACTATCC TTCCCTCCAG GCCATGGGAT GGGAAGGCAA TGAAGTAGGG 
TCCTTCCACG TCCACTCTGG GGCCTGGGTT TGCTCCCAGT TTCCGGGCTA CCGAGGATTT 
CAGTATGTGC TGGAATGCGA TCACCATTCC GGTGACTACA AACATTTCCG GGAGTGGGGC 
TCTCATGCCC CGACCTTCCA GGTGCAGAGC ATCCGCAGGA TCCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001877.1
CDS	36..626
Translation	MTLQCTKSAGPWKMVVWDEDGFQGRRHEFTAECPSVLELGFETVRSLKVLSGAWVGFEHAGFQGQQYILERGEYPSWDAWGGNTAYPAERLTSFRPAACANHRDSRLTIFEQENFLGKKGELSDDYPSLQAMGWEGNEVGSFHVHSGAWVCSQFPGYRGFQYVLECDHHSGDYKHFREWGSHAPTFQVQSIRRIQQ

Target ORF information:

RefSeq Version	NM_001886.2
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin beta A4 (CRYBA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001886.2

ORF Insert Sequence:

ATGACCCTGC AATGCACAAA GTCAGCGGGA CCCTGGAAGA TGGTGGTGTG GGATGAGGAC 
GGCTTCCAGG GCCGGCGGCA CGAGTTCACG GCCGAGTGCC CCAGCGTGCT GGAGCTTGGC 
TTCGAGACTG TGCGATCTTT GAAAGTGCTG AGTGGAGCGT GGGTGGGCTT TGAGCATGCT 
GGCTTCCAAG GGCAGCAGTA CATTCTGGAA CGAGGCGAAT ATCCAAGCTG GGATGCCTGG 
GGCGGCAACA CGGCCTACCC CGCCGAGAGG CTCACCTCCT TCCGGCCTGC GGCCTGTGCT 
AACCACCGTG ACTCGAGGCT GACAATCTTC GAGCAAGAGA ACTTCCTGGG CAAGAAAGGA 
GAGCTGAGCG ATGACTATCC TTCCCTCCAG GCCATGGGAT GGGAAGGCAA TGAAGTAGGG 
TCCTTCCACG TCCACTCTGG GGCCTGGGTT TGCTCCCAGT TTCCGGGCTA CCGAGGATTT 
CAGTATGTGC TGGAATGCGA TCACCATTCC GGTGACTACA AACATTTCCG GGAGTGGGGC 
TCTCATGCCC CGACCTTCCA GGTGCAGAGC ATCCGCAGGA TCCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBA4 ( XM_006724140.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724203.1 Homo sapiens crystallin beta A4 (CRYBA4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu53412
Clone ID Related Accession (Same CDS sequence)	XM_006724140.3
Accession Version	XM_006724140.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	beta-crystallin A4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006724140.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGGG CCACAATGAC CCTGCAATGC ACAAAGTCAG CGGGACCCTG GAAGATGGTG 
GTGTGGGATG AGGACGGCTT CCAGGGCCGG CGGCACGAGT TCACGGCCGA GTGCCCCAGC 
GTGCTGGAGC TTGGCTTCGA GACTGTGCGA TCTTTGAAAG TGCTGAGTGG AGCGTGGGTG 
GGCTTTGAGC ATGCTGGCTT CCAAGGGCAG CAGTACATTC TGGAACGAGG CGAATATCCA 
AGCTGGGATG CCTGGGGCGG CAACACGGCC TACCCCGCCG AGAGGCTCAC CTCCTTCCGG 
CCTGCGGCCT GTGCTAACCA CCGTGACTCG AGGCTGACAA TCTTCGAGCA AGAGAACTTC 
CTGGGCAAGA AAGGAGAGCT GAGCGATGAC TATCCTTCCC TCCAGGCCAT GGGATGGGAA 
GGCAATGAAG TAGGGTCCTT CCACGTCCAC TCTGGGGCCT GGGTTTGCTC CCAGTTTCCG 
GGCTACCGAG GATTTCAGTA TGTGCTGGAA TGCGATCACC ATTCCGGTGA CTACAAACAT 
TTCCGGGAGT GGGGCTCTCA TGCCCCGACC TTCCAGGTGC AGAGCATCCG CAGGATCCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724203.1
CDS	79..684
Translation	MEGATMTLQCTKSAGPWKMVVWDEDGFQGRRHEFTAECPSVLELGFETVRSLKVLSGAWVGFEHAGFQGQQYILERGEYPSWDAWGGNTAYPAERLTSFRPAACANHRDSRLTIFEQENFLGKKGELSDDYPSLQAMGWEGNEVGSFHVHSGAWVCSQFPGYRGFQYVLECDHHSGDYKHFREWGSHAPTFQVQSIRRIQQ

Target ORF information:

RefSeq Version	XM_006724140.3
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin beta A4 (CRYBA4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724140.3

ORF Insert Sequence:

ATGGAAGGGG CCACAATGAC CCTGCAATGC ACAAAGTCAG CGGGACCCTG GAAGATGGTG 
GTGTGGGATG AGGACGGCTT CCAGGGCCGG CGGCACGAGT TCACGGCCGA GTGCCCCAGC 
GTGCTGGAGC TTGGCTTCGA GACTGTGCGA TCTTTGAAAG TGCTGAGTGG AGCGTGGGTG 
GGCTTTGAGC ATGCTGGCTT CCAAGGGCAG CAGTACATTC TGGAACGAGG CGAATATCCA 
AGCTGGGATG CCTGGGGCGG CAACACGGCC TACCCCGCCG AGAGGCTCAC CTCCTTCCGG 
CCTGCGGCCT GTGCTAACCA CCGTGACTCG AGGCTGACAA TCTTCGAGCA AGAGAACTTC 
CTGGGCAAGA AAGGAGAGCT GAGCGATGAC TATCCTTCCC TCCAGGCCAT GGGATGGGAA 
GGCAATGAAG TAGGGTCCTT CCACGTCCAC TCTGGGGCCT GGGTTTGCTC CCAGTTTCCG 
GGCTACCGAG GATTTCAGTA TGTGCTGGAA TGCGATCACC ATTCCGGTGA CTACAAACAT 
TTCCGGGAGT GGGGCTCTCA TGCCCCGACC TTCCAGGTGC AGAGCATCCG CAGGATCCAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBA4 ( XM_017028598.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884087.1 Homo sapiens crystallin beta A4 (CRYBA4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu93369
Clone ID Related Accession (Same CDS sequence)	XM_017028598.1
Accession Version	XM_017028598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	beta-crystallin A4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCTG GGCCTATCTC GGAAGGGGCC ACAATGACCC TGCAATGCAC AAAGTCAGCG 
GGACCCTGGA AGATGGTGGT GTGGGATGAG GACGGCTTCC AGGGCCGGCG GCACGAGTTC 
ACGGCCGAGT GCCCCAGCGT GCTGGAGCTT GGCTTCGAGA CTGTGCGATC TTTGAAAGTG 
CTGAGTGGAG CGTGGGTGGG CTTTGAGCAT GCTGGCTTCC AAGGGCAGCA GTACATTCTG 
GAACGAGGCG AATATCCAAG CTGGGATGCC TGGGGCGGCA ACACGGCCTA CCCCGCCGAG 
AGGCTCACCT CCTTCCGGCC TGCGGCCTGT GCTAACCACC GTGACTCGAG GCTGACAATC 
TTCGAGCAAG AGAACTTCCT GGGCAAGAAA GGAGAGCTGA GCGATGACTA TCCTTCCCTC 
CAGGCCATGG GATGGGAAGG CAATGAAGTA GGGTCCTTCC ACGTCCACTC TGGGGCCTGG 
GTTTGCTCCC AGTTTCCGGG CTACCGAGGA TTTCAGTATG TGCTGGAATG CGATCACCAT 
TCCGGTGACT ACAAACATTT CCGGGAGTGG GGCTCTCATG CCCCGACCTT CCAGGTGCAG 
AGCATCCGCA GGATCCAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884087.1
CDS	45..668
Translation	MFPGPISEGATMTLQCTKSAGPWKMVVWDEDGFQGRRHEFTAECPSVLELGFETVRSLKVLSGAWVGFEHAGFQGQQYILERGEYPSWDAWGGNTAYPAERLTSFRPAACANHRDSRLTIFEQENFLGKKGELSDDYPSLQAMGWEGNEVGSFHVHSGAWVCSQFPGYRGFQYVLECDHHSGDYKHFREWGSHAPTFQVQSIRRIQQ

Target ORF information:

RefSeq Version	XM_017028598.1
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin beta A4 (CRYBA4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028598.1

ORF Insert Sequence:

ATGTTCCCTG GGCCTATCTC GGAAGGGGCC ACAATGACCC TGCAATGCAC AAAGTCAGCG 
GGACCCTGGA AGATGGTGGT GTGGGATGAG GACGGCTTCC AGGGCCGGCG GCACGAGTTC 
ACGGCCGAGT GCCCCAGCGT GCTGGAGCTT GGCTTCGAGA CTGTGCGATC TTTGAAAGTG 
CTGAGTGGAG CGTGGGTGGG CTTTGAGCAT GCTGGCTTCC AAGGGCAGCA GTACATTCTG 
GAACGAGGCG AATATCCAAG CTGGGATGCC TGGGGCGGCA ACACGGCCTA CCCCGCCGAG 
AGGCTCACCT CCTTCCGGCC TGCGGCCTGT GCTAACCACC GTGACTCGAG GCTGACAATC 
TTCGAGCAAG AGAACTTCCT GGGCAAGAAA GGAGAGCTGA GCGATGACTA TCCTTCCCTC 
CAGGCCATGG GATGGGAAGG CAATGAAGTA GGGTCCTTCC ACGTCCACTC TGGGGCCTGG 
GTTTGCTCCC AGTTTCCGGG CTACCGAGGA TTTCAGTATG TGCTGGAATG CGATCACCAT 
TCCGGTGACT ACAAACATTT CCGGGAGTGG GGCTCTCATG CCCCGACCTT CCAGGTGCAG 
AGCATCCGCA GGATCCAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Partial duplication of the CRYBB1-CRYBA4 locus is associated with autosomal dominant congenital cataract.
European journal of human genetics : EJHG25(6)711-718(2017 06)
Siggs OM,Javadiyan S,Sharma S,Souzeau E,Lower KM,Taranath DA,Black J,Pater J,Willoughby JG,Burdon KP,Craig JE

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
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CRYBA4 cDNA ORF clone, Homo sapiens(human)

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