CRYBA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRYBA1
Entrez Gene ID	1411
Full Name	crystallin beta A1
Synonyms	CRYB1,CTRCT10
General protein information	Preferred Names crystallin beta A1 Names beta-crystallin A3 beta crystallin A3 chain transcript CN beta crystallin A3 chain transcript LAM beta crystallin A3 chain transcript PS beta crystallin A3 chain transcript TC crystallin beta A3/A1 crystallin, beta A3 eye lens structural protein truncated beta crystallin A3/A1 chain truncated beta-crystallin A3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q11.2
Summary	Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'. [provided by RefSeq, Jul 2008].
Disorder MIM:	123610
Disorder Html:	Cataract 10, multiple types, 600881 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005208.4	NP_005199.2	beta-crystallin A3
XM_017024198.1	XP_016879687.1	beta-crystallin A3 isoform X1

Pathways from BioSystems

Homologies

	CRYBA1	XP_001107784.1
Gallus gallus (chicken)	CRYBA1	NP_990833.2
Pan troglodytes (chimpanzee)	CRYBA1	XP_523590.2
Bos taurus (cattle)	CRYBA1	NP_776948.1
Mus musculus (house mouse)	Cryba1	NP_034095.1
Xenopus tropicalis (tropical clawed frog)	cryba1	NP_001096535.1
Homo sapiens (human)	CRYBA1	NP_005199.2
Rattus norvegicus (Norway rat)	Cryba1	NP_037188.1
Canis lupus familiaris (dog)	CRYBA1	NP_001074368.1
Danio rerio (zebrafish)	cryba1a	NP_001002410.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of a De Novo 3bp Deletion in CRYBA1/A3 Gene in Autosomal Dominant Congenital Cataract.
Mohebi M, Akbari A, Babaei N, Sadeghi A, Heidari M
Acta medica Iranica54(12)778-783(2016 Dec)

Human ?A3/A1-crystallin splicing mutation causes cataracts by activating the unfolded protein response and inducing apoptosis in differentiating lens fiber cells.
Ma Z, Yao W, Chan CC, Kannabiran C, Wawrousek E, Hejtmancik JF
Biochimica et biophysica acta1862(6)1214-27(2016 Jun)

?A3/A1-crystallin and persistent fetal vasculature (PFV) disease of the eye.
Zigler JS Jr, Valapala M, Shang P, Hose S, Goldberg MF, Sinha D
Biochimica et biophysica acta1860(1 Pt B)287-98(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We identified a de novo in-frame 3-bp deletion in the proband with an autosomal dominant congenital cataract, but not in her parents, in an ian family. This mutation has occurred de novo on a paternal gamete during spermatogenesis. The in-silico results predicted the interaction of CRYBA1 protein with the other CRY as well as proteins responsible for eye cell signaling.
Title: Identification of a De Novo 3bp Deletion in CRYBA1/A3 Gene in Autosomal Dominant Congenital Cataract.

The findings suggest that impaired endolysosomal signaling in ocular astrocytes can cause PFV disease, by adversely affecting the vascular remodeling processes essential to ocular development, including regression of the fetal vasculature. [review]
Title: ?A3/A1-crystallin and persistent fetal vasculature (PFV) disease of the eye.

Data indicate that alpha-crystallin B chain and beta-crystallin A3-cyrstallins dissociate to the monomers upon racemization of d-aspartic acids (Asp).
Title: Alpha B- and ?A3-crystallins containing d-aspartic acids exist in a monomeric state.

association between a frameshift mutation in exon 6 of CRYBA1/A3 and congenital cataracts
Title: Congenital cataracts due to a novel 2?bp deletion in CRYBA1/A3.

A splice site mutation (c.215+1G>A) at the first base of intron 3 of the crystallin beta A3/A1 (CRYBA3/A1) gene has been identified in Chinese congenital polymorphic cataract patients.
Title: Congenital polymorphic cataract associated with a G to A splice site mutation in the human beta-crystallin gene CRY?A3/A1.

The following CRYBA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRYBA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu18412	NM_005208.4 Latest version!	Homo sapiens crystallin beta A1 (CRYBA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
OHu86601	XM_017024198.1 Latest version!	Homo sapiens crystallin beta A1 (CRYBA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRYBA1 ( NM_005208.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005199.2 Homo sapiens crystallin beta A1 (CRYBA1), mRNA.

Price & Availability↑

CloneID	OHu18412
Clone ID Related Accession (Same CDS sequence)	NM_005208.4
Accession Version	NM_005208.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	beta-crystallin A3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005412.8, BC113693.1 and BU735083.1. This sequence is a reference standard in the RefSeqGene project. On Mar 12, 2008 this sequence version replaced NM_005208.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BY794946.2, BC113693.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148093, SAMEA2148874 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGACCC AGGCTGAGCA GCAGGAGCTG GAAACCCTTC CAACCACCAA GATGGCTCAG 
ACCAACCCTA CGCCGGGGTC CCTGGGGCCA TGGAAGATAA CCATCTATGA TCAGGAGAAC 
TTTCAGGGCA AGAGGATGGA GTTCACCAGC TCCTGTCCAA ATGTCTCTGA GCGCAGTTTT 
GATAATGTCC GGTCCCTGAA GGTGGAAAGT GGCGCCTGGA TTGGTTATGA GCATACCAGC 
TTCTGTGGGC AACAGTTTAT CCTGGAGAGA GGAGAATACC CTCGCTGGGA TGCCTGGAGT 
GGGAGTAATG CCTACCACAT TGAGCGTCTC ATGTCCTTCC GCCCCATCTG TTCAGCTAAT 
CATAAGGAGT CTAAGATGAC CATCTTTGAG AAGGAAAACT TTATTGGACG CCAGTGGGAG 
ATCTCTGACG ACTACCCCTC CTTGCAAGCC ATGGGCTGGT TCAACAACGA AGTCGGCTCC 
ATGAAGATAC AAAGTGGGGC CTGGGTTTGC TACCAATATC CTGGATATCG TGGGTATCAG 
TATATCTTGG AATGTGACCA TCATGGAGGA GACTATAAAC ATTGGAGAGA GTGGGGCTCT 
CATGCCCAGA CTTCGCAGAT CCAATCGATT CGCCGAATCC AACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005199.2
CDS	8..655
Translation	METQAEQQELETLPTTKMAQTNPTPGSLGPWKITIYDQENFQGKRMEFTSSCPNVSERSFDNVRSLKVESGAWIGYEHTSFCGQQFILERGEYPRWDAWSGSNAYHIERLMSFRPICSANHKESKMTIFEKENFIGRQWEISDDYPSLQAMGWFNNEVGSMKIQSGAWVCYQYPGYRGYQYILECDHHGGDYKHWREWGSHAQTSQIQSIRRIQQ

Target ORF information:

RefSeq Version	NM_005208.4
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin beta A1 (CRYBA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005208.4

ORF Insert Sequence:

ATGGAGACCC AGGCTGAGCA GCAGGAGCTG GAAACCCTTC CAACCACCAA GATGGCTCAG 
ACCAACCCTA CGCCGGGGTC CCTGGGGCCA TGGAAGATAA CCATCTATGA TCAGGAGAAC 
TTTCAGGGCA AGAGGATGGA GTTCACCAGC TCCTGTCCAA ATGTCTCTGA GCGCAGTTTT 
GATAATGTCC GGTCCCTGAA GGTGGAAAGT GGCGCCTGGA TTGGTTATGA GCATACCAGC 
TTCTGTGGGC AACAGTTTAT CCTGGAGAGA GGAGAATACC CTCGCTGGGA TGCCTGGAGT 
GGGAGTAATG CCTACCACAT TGAGCGTCTC ATGTCCTTCC GCCCCATCTG TTCAGCTAAT 
CATAAGGAGT CTAAGATGAC CATCTTTGAG AAGGAAAACT TTATTGGACG CCAGTGGGAG 
ATCTCTGACG ACTACCCCTC CTTGCAAGCC ATGGGCTGGT TCAACAACGA AGTCGGCTCC 
ATGAAGATAC AAAGTGGGGC CTGGGTTTGC TACCAATATC CTGGATATCG TGGGTATCAG 
TATATCTTGG AATGTGACCA TCATGGAGGA GACTATAAAC ATTGGAGAGA GTGGGGCTCT 
CATGCCCAGA CTTCGCAGAT CCAATCGATT CGCCGAATCC AACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYBA1 ( XM_017024198.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879687.1 Homo sapiens crystallin beta A1 (CRYBA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu86601
Clone ID Related Accession (Same CDS sequence)	XM_017024198.1
Accession Version	XM_017024198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	beta-crystallin A3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 18% of CDS bases ##RefSeq-Attributes-END##

ATGGAGACCC AGGCTGAGCA GCAGGAGCTG GTCTTTTTCA ATGTCCCCCT GATCCCTCAT 
CTCCACATGT GCTCAGCACG ATGCTATACC AATCCCCTAG AAGACCGAGG CCTTGCCATT 
GAGGTGCTCA CCAGGACATC CAACCAGAAC ACAGATTGTC AAAATAGCAA CAACAACAAC 
AACAAACCCC GAGGCACAGC TAGTGAGCAG CAGAGCCAGA ATTTCAACCA AAGTCTGTCT 
TGCAGACTTC AAGTCTCCTT TACCTTTCAA GGCATTCCCT CACCTCCCCC TCCTCCCAAG 
AGGCCACATC ATTCGTGTGT GCTCTGTCTT CCAGAAACCC TTCCAACCAC CAAGATGGCT 
CAGACCAACC CTACGCCGGG GTCCCTGGGG CCATGGAAGA TAACCATCTA TGATCAGGAG 
AACTTTCAGG GCAAGAGGAT GGAGTTCACC AGCTCCTGTC CAAATGTCTC TGAGCGCAGT 
TTTGATAATG TCCGGTCCCT GAAGGTGGAA AGTGGCGCCT GGATTGGTTA TGAGCATACC 
AGCTTCTGTG GGCAACAGTT TATCCTGGAG AGAGGAGAAT ACCCTCGCTG GGATGCCTGG 
AGTGGGAGTA ATGCCTACCA CATTGAGCGT CTCATGTCCT TCCGCCCCAT CTGTTCAGCT 
AATCATAAGG AGTCTAAGAT GACCATCTTT GAGAAGGAAA ACTTTATTGG ACGCCAGTGG 
GAGATCTCTG ACGACTACCC CTCCTTGCAA GCCATGGGCT GGTTCAACAA CGAAGTCGGC 
TCCATGAAGA TACAAAGTGG GGCCTGGGTT TGCTACCAAT ATCCTGGATA TCGTGGGTAT 
CAGTATATCT TGGAATGTGA CCATCATGGA GGAGACTATA AACATTGGAG AGAGTGGGGC 
TCTCATGCCC AGACTTCGCA GATCCAATCG ATTCGCCGAA TCCAACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879687.1
CDS	1..951
Translation	METQAEQQELVFFNVPLIPHLHMCSARCYTNPLEDRGLAIEVLTRTSNQNTDCQNSNNNNNKPRGTASEQQSQNFNQSLSCRLQVSFTFQGIPSPPPPPKRPHHSCVLCLPETLPTTKMAQTNPTPGSLGPWKITIYDQENFQGKRMEFTSSCPNVSERSFDNVRSLKVESGAWIGYEHTSFCGQQFILERGEYPRWDAWSGSNAYHIERLMSFRPICSANHKESKMTIFEKENFIGRQWEISDDYPSLQAMGWFNNEVGSMKIQSGAWVCYQYPGYRGYQYILECDHHGGDYKHWREWGSHAQTSQIQSIRRIQQ

Target ORF information:

RefSeq Version	XM_017024198.1
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin beta A1 (CRYBA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024198.1

ORF Insert Sequence:

ATGGAGACCC AGGCTGAGCA GCAGGAGCTG GTCTTTTTCA ATGTCCCCCT GATCCCTCAT 
CTCCACATGT GCTCAGCACG ATGCTATACC AATCCCCTAG AAGACCGAGG CCTTGCCATT 
GAGGTGCTCA CCAGGACATC CAACCAGAAC ACAGATTGTC AAAATAGCAA CAACAACAAC 
AACAAACCCC GAGGCACAGC TAGTGAGCAG CAGAGCCAGA ATTTCAACCA AAGTCTGTCT 
TGCAGACTTC AAGTCTCCTT TACCTTTCAA GGCATTCCCT CACCTCCCCC TCCTCCCAAG 
AGGCCACATC ATTCGTGTGT GCTCTGTCTT CCAGAAACCC TTCCAACCAC CAAGATGGCT 
CAGACCAACC CTACGCCGGG GTCCCTGGGG CCATGGAAGA TAACCATCTA TGATCAGGAG 
AACTTTCAGG GCAAGAGGAT GGAGTTCACC AGCTCCTGTC CAAATGTCTC TGAGCGCAGT 
TTTGATAATG TCCGGTCCCT GAAGGTGGAA AGTGGCGCCT GGATTGGTTA TGAGCATACC 
AGCTTCTGTG GGCAACAGTT TATCCTGGAG AGAGGAGAAT ACCCTCGCTG GGATGCCTGG 
AGTGGGAGTA ATGCCTACCA CATTGAGCGT CTCATGTCCT TCCGCCCCAT CTGTTCAGCT 
AATCATAAGG AGTCTAAGAT GACCATCTTT GAGAAGGAAA ACTTTATTGG ACGCCAGTGG 
GAGATCTCTG ACGACTACCC CTCCTTGCAA GCCATGGGCT GGTTCAACAA CGAAGTCGGC 
TCCATGAAGA TACAAAGTGG GGCCTGGGTT TGCTACCAAT ATCCTGGATA TCGTGGGTAT 
CAGTATATCT TGGAATGTGA CCATCATGGA GGAGACTATA AACATTGGAG AGAGTGGGGC 
TCTCATGCCC AGACTTCGCA GATCCAATCG ATTCGCCGAA TCCAACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of a De Novo 3bp Deletion in CRYBA1/A3 Gene in Autosomal Dominant Congenital Cataract.
Acta medica ica54(12)778-783(2016 Dec)
Mohebi M,Akbari A,Babaei N,Sadeghi A,Heidari M

Human ?A3/A1-crystallin splicing mutation causes cataracts by activating the unfolded protein response and inducing apoptosis in differentiating lens fiber cells.
Biochimica et biophysica acta1862(6)1214-27(2016 Jun)
Ma Z,Yao W,Chan CC,Kannabiran C,Wawrousek E,Hejtmancik JF

?A3/A1-crystallin and persistent fetal vasculature (PFV) disease of the eye.
Biochimica et biophysica acta1860(1 Pt B)287-98(2016 Jan)
Zigler JS Jr,Valapala M,Shang P,Hose S,Goldberg MF,Sinha D

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CRYBA1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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