CPT2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CPT2
Entrez Gene ID	1376
Full Name	carnitine palmitoyltransferase 2
Synonyms	CPT1,CPTASE,IIAE4
General protein information	Preferred Names carnitine palmitoyltransferase 2 Names carnitine O-palmitoyltransferase 2, mitochondrial CPT II carnitine palmitoyltransferase II testicular secretory protein Li 13
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p32.3
Summary	The protein encoded by this gene is a nuclear protein which is transported to the mitochondrial inner membrane. Together with carnitine palmitoyltransferase I, the encoded protein oxidizes long-chain fatty acids in the mitochondria. Defects in this gene are associated with mitochondrial long-chain fatty-acid (LCFA) oxidation disorders. [provided by RefSeq, Jul 2008].
Disorder MIM:	600650
Disorder Html:	CPT II deficiency, myopathic, stress-induced, 255110 (3); CPT II deficiency, infantile, 600649 (3); CPT II deficiency, lethal neonatal, 608836 (3); {Encephalopathy, acute, infection-induced, 4, susceptibility to}, 614212 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000098.3	NP_000089.1	carnitine O-palmitoyltransferase 2, mitochondrial isoform 1 precursor
NM_001330589.1	NP_001317518.1	carnitine O-palmitoyltransferase 2, mitochondrial isoform 2 precursor
NM_001330589.2	NP_001317518.1	carnitine O-palmitoyltransferase 2, mitochondrial isoform 2 precursor
NM_000098.2	NP_000089.1	carnitine O-palmitoyltransferase 2, mitochondrial isoform 1 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CPT2	NP_001039354.1
Caenorhabditis elegans (roundworm)	cpt-2	NP_001040977.1
Saccharomyces cerevisiae (baker's yeast)	YAT1	NP_009420.1
Eremothecium gossypii	AGOS_AER224W	NP_985081.1
Magnaporthe oryzae (rice blast fungus)	MGG_06981	XP_003709730.1
	CPT2	XP_001112489.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008907	XP_552786.2
Homo sapiens (human)	CPT2	NP_000089.1
Pan troglodytes (chimpanzee)	CPT2	XP_001148628.1
Gallus gallus (chicken)	CPT2	NP_001026458.2
Danio rerio (zebrafish)	cpt2	NP_001007448.1
Neurospora crassa	NCU01611	XP_956522.1
Rattus norvegicus (Norway rat)	Cpt2	NP_037062.1
Drosophila melanogaster (fruit fly)	CG2107	NP_647756.1
Xenopus tropicalis (tropical clawed frog)	cpt2	NP_989193.1
Canis lupus familiaris (dog)	CPT2	XP_546705.1
Mus musculus (house mouse)	Cpt2	NP_034079.2
Kluyveromyces lactis	KLLA0C04169g	XP_452383.1

Gene Ontology
Bibliography

Related articles in PubMed

Muscle Carnitine Palmitoyltransferase II Deficiency: A Review of Enzymatic Controversy and Clinical Features.
Lehmann D, Motlagh L, Robaa D, Zierz S
International journal of molecular sciences18(1)(2017 Jan)

Genetic evaluation of AMPD1, CPT2, and PGYM metabolic enzymes in patients with chronic fatigue syndrome.
Maltese PE, Venturini L, Poplavskaya E, Bertelli M, Cecchin S, Granato M, Nikulina SY, Salmina A, Aksyutina N, Capelli E, Ricevuti G, Lorusso L
Genetics and molecular research : GMR15(3)(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The clinical presentation of patients with muscle carnitine palmitoyltransferase II deficiency is discussed in this review in line with enzymatic features. The thermolability of the mutant enzyme might explain why symptoms in muscle CPT II deficiency mainly occur during prolonged exercise, infections and exposure to cold. [review]
Title: Muscle Carnitine Palmitoyltransferase II Deficiency: A Review of Enzymatic Controversy and Clinical Features.

this study shows that upregulation of the citrate pathway and down-regulation of carnitine/acylcarnitine carrier gene in cells from children with Down syndrome
Title: The contribution of the citrate pathway to oxidative stress in Down syndrome.

Variations in AMPD1, CPT2, and PGYM genes are not associated with the onset, susceptibility, or severity of chronic fatigue syndrome.
Title: Genetic evaluation of AMPD1, CPT2, and PGYM metabolic enzymes in patients with chronic fatigue syndrome.

polymorphism may be associated with severity of EV71 infection
Title: Carnitine palmitoyl transferase 2 polymorphism may be associated with enterovirus 71 severe infection in a Chinese population.

CPT2 is active inside the mitochondrial matrix to recover acyl-CoA from a process generally known as the carnitine shuttle. This protein is expressed in a constitutive way in all cells and tissues.
Title: The Carnitine Palmitoyl Transferase (CPT) System and Possible Relevance for Neuropsychiatric and Neurological Conditions.

The following CPT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CPT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17939	NM_000098.3 Latest version!	Homo sapiens carnitine palmitoyltransferase 2 (CPT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu33029	NM_001330589.2 Latest version!	Homo sapiens carnitine palmitoyltransferase 2 (CPT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu33029	NM_001330589.1	Homo sapiens carnitine palmitoyltransferase 2 (CPT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu17939	NM_000098.2	Homo sapiens carnitine palmitoyltransferase 2 (CPT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CPT2 ( NM_000098.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000089.1 Homo sapiens carnitine palmitoyltransferase 2 (CPT2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17939
Clone ID Related Accession (Same CDS sequence)	NM_000098.3 , NM_000098.2
Accession Version	NM_000098.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	carnitine O-palmitoyltransferase 2, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U09648.1 and BU629852.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000098.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005553.1, U09648.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000371486.4/ ENSP00000360541.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCCCC GCCTGCTGCT GCGCGCCTGG CCCCGGGGCC CCGCGGTTGG TCCGGGAGCC 
CCCAGTCGGC CCCTCAGCGC CGGCTCCGGG CCCGGCCAGT ACCTGCAGCG CAGCATCGTG 
CCCACCATGC ACTACCAGGA CAGCCTGCCC AGGCTGCCTA TTCCCAAACT TGAAGACACC 
ATTAGGAGAT ACCTCAGTGC ACAGAAGCCT CTCTTGAATG ATGGCCAGTT CAGGAAAACA 
GAACAATTTT GCAAGAGTTT TGAAAATGGG ATTGGAAAAG AACTGCATGA GCAGCTGGTT 
GCTCTGGACA AACAGAATAA ACATACAAGC TACATTTCGG GACCCTGGTT TGATATGTAC 
CTATCTGCTC GAGACTCCGT TGTTCTGAAC TTTAATCCAT TTATGGCTTT CAATCCTGAC 
CCAAAATCTG AGTATAATGA CCAGCTCACC CGGGCAACCA ACATGACTGT TTCTGCCATC 
CGGTTTCTGA AGACACTCCG GGCTGGCCTT CTGGAGCCAG AAGTGTTCCA CTTGAACCCT 
GCAAAAAGTG ACACTATCAC CTTCAAGAGA CTCATACGCT TTGTGCCTTC CTCTCTGTCC 
TGGTATGGGG CCTACCTGGT CAATGCGTAT CCCCTGGATA TGTCCCAGTA TTTTCGGCTT 
TTCAACTCAA CTCGTTTACC CAAACCCAGT CGGGATGAAC TCTTCACTGA TGACAAGGCC 
AGACACCTCC TGGTCCTAAG GAAAGGAAAT TTTTATATCT TTGATGTCCT GGATCAAGAT 
GGGAACATTG TGAGCCCCTC GGAAATCCAG GCACATCTGA AGTACATTCT CTCAGACAGC 
AGCCCCGCCC CCGAGTTTCC CCTGGCATAC CTGACCAGTG AGAACCGAGA CATCTGGGCA 
GAGCTCAGGC AGAAGCTGAT GAGTAGTGGC AATGAGGAGA GCCTGAGGAA AGTGGACTCG 
GCAGTGTTCT GTCTCTGCCT AGATGACTTC CCCATTAAGG ACCTTGTCCA CTTGTCCCAC 
AATATGCTGC ATGGGGATGG CACAAACCGC TGGTTTGATA AATCCTTTAA CCTCATTATC 
GCCAAGGATG GCTCTACTGC CGTCCACTTT GAGCACTCTT GGGGTGATGG TGTGGCAGTG 
CTCAGATTTT TTAATGAAGT ATTTAAAGAC AGCACTCAGA CCCCTGCCGT CACTCCACAG 
AGCCAGCCAG CTACCACTGA CTCTACTGTC ACGGTGCAGA AACTCAACTT CGAGCTGACT 
GATGCCTTAA AGACTGGCAT CACAGCTGCT AAGGAAAAGT TTGATGCCAC CATGAAAACC 
CTCACTATTG ACTGCGTCCA GTTTCAGAGA GGAGGCAAAG AATTCCTGAA GAAGCAAAAG 
CTGAGCCCTG ACGCAGTTGC CCAGCTGGCA TTCCAGATGG CCTTCCTGCG GCAGTACGGG 
CAGACAGTGG CCACCTACGA GTCCTGTAGC ACTGCCGCAT TCAAGCACGG CCGCACTGAG 
ACCATCCGCC CGGCCTCCGT CTATACAAAG AGGTGCTCTG AGGCCTTTGT CAGGGAGCCC 
TCCAGGCACA GTGCTGGTGA GCTTCAGCAG ATGATGGTTG AGTGCTCCAA GTACCATGGC 
CAGCTGACCA AAGAAGCAGC AATGGGCCAG GGCTTTGACC GACACTTGTT TGCTCTGCGG 
CATCTGGCAG CAGCCAAAGG GATCATCTTG CCTGAGCTCT ACCTGGACCC TGCATACGGG 
CAGATAAACC ACAATGTCCT GTCCACGAGC ACACTGAGCA GCCCAGCAGT GAACCTTGGG 
GGCTTTGCCC CTGTGGTCTC TGATGGCTTT GGTGTTGGGT ATGCTGTTCA TGACAACTGG 
ATAGGCTGCA ATGTCTCTTC CTACCCAGGC CGCAATGCCC GGGAGTTTCT CCAATGTGTG 
GAGAAGGCCT TAGAAGACAT GTTTGATGCC TTAGAAGGCA AATCCATCAA AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000089.1
CDS	121..2097
Translation	MVPRLLLRAWPRGPAVGPGAPSRPLSAGSGPGQYLQRSIVPTMHYQDSLPRLPIPKLEDTIRRYLSAQKPLLNDGQFRKTEQFCKSFENGIGKELHEQLVALDKQNKHTSYISGPWFDMYLSARDSVVLNFNPFMAFNPDPKSEYNDQLTRATNMTVSAIRFLKTLRAGLLEPEVFHLNPAKSDTITFKRLIRFVPSSLSWYGAYLVNAYPLDMSQYFRLFNSTRLPKPSRDELFTDDKARHLLVLRKGNFYIFDVLDQDGNIVSPSEIQAHLKYILSDSSPAPEFPLAYLTSENRDIWAELRQKLMSSGNEESLRKVDSAVFCLCLDDFPIKDLVHLSHNMLHGDGTNRWFDKSFNLIIAKDGSTAVHFEHSWGDGVAVLRFFNEVFKDSTQTPAVTPQSQPATTDSTVTVQKLNFELTDALKTGITAAKEKFDATMKTLTIDCVQFQRGGKEFLKKQKLSPDAVAQLAFQMAFLRQYGQTVATYESCSTAAFKHGRTETIRPASVYTKRCSEAFVREPSRHSAGELQQMMVECSKYHGQLTKEAAMGQGFDRHLFALRHLAAAKGIILPELYLDPAYGQINHNVLSTSTLSSPAVNLGGFAPVVSDGFGVGYAVHDNWIGCNVSSYPGRNAREFLQCVEKALEDMFDALEGKSIKS

Target ORF information:

RefSeq Version	NM_000098.3
Organism	Homo sapiens(human)
Definition	Homo sapiens carnitine palmitoyltransferase 2 (CPT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000098.3

ORF Insert Sequence:

ATGGTGCCCC GCCTGCTGCT GCGCGCCTGG CCCCGGGGCC CCGCGGTTGG TCCGGGAGCC 
CCCAGTCGGC CCCTCAGCGC CGGCTCCGGG CCCGGCCAGT ACCTGCAGCG CAGCATCGTG 
CCCACCATGC ACTACCAGGA CAGCCTGCCC AGGCTGCCTA TTCCCAAACT TGAAGACACC 
ATTAGGAGAT ACCTCAGTGC ACAGAAGCCT CTCTTGAATG ATGGCCAGTT CAGGAAAACA 
GAACAATTTT GCAAGAGTTT TGAAAATGGG ATTGGAAAAG AACTGCATGA GCAGCTGGTT 
GCTCTGGACA AACAGAATAA ACATACAAGC TACATTTCGG GACCCTGGTT TGATATGTAC 
CTATCTGCTC GAGACTCCGT TGTTCTGAAC TTTAATCCAT TTATGGCTTT CAATCCTGAC 
CCAAAATCTG AGTATAATGA CCAGCTCACC CGGGCAACCA ACATGACTGT TTCTGCCATC 
CGGTTTCTGA AGACACTCCG GGCTGGCCTT CTGGAGCCAG AAGTGTTCCA CTTGAACCCT 
GCAAAAAGTG ACACTATCAC CTTCAAGAGA CTCATACGCT TTGTGCCTTC CTCTCTGTCC 
TGGTATGGGG CCTACCTGGT CAATGCGTAT CCCCTGGATA TGTCCCAGTA TTTTCGGCTT 
TTCAACTCAA CTCGTTTACC CAAACCCAGT CGGGATGAAC TCTTCACTGA TGACAAGGCC 
AGACACCTCC TGGTCCTAAG GAAAGGAAAT TTTTATATCT TTGATGTCCT GGATCAAGAT 
GGGAACATTG TGAGCCCCTC GGAAATCCAG GCACATCTGA AGTACATTCT CTCAGACAGC 
AGCCCCGCCC CCGAGTTTCC CCTGGCATAC CTGACCAGTG AGAACCGAGA CATCTGGGCA 
GAGCTCAGGC AGAAGCTGAT GAGTAGTGGC AATGAGGAGA GCCTGAGGAA AGTGGACTCG 
GCAGTGTTCT GTCTCTGCCT AGATGACTTC CCCATTAAGG ACCTTGTCCA CTTGTCCCAC 
AATATGCTGC ATGGGGATGG CACAAACCGC TGGTTTGATA AATCCTTTAA CCTCATTATC 
GCCAAGGATG GCTCTACTGC CGTCCACTTT GAGCACTCTT GGGGTGATGG TGTGGCAGTG 
CTCAGATTTT TTAATGAAGT ATTTAAAGAC AGCACTCAGA CCCCTGCCGT CACTCCACAG 
AGCCAGCCAG CTACCACTGA CTCTACTGTC ACGGTGCAGA AACTCAACTT CGAGCTGACT 
GATGCCTTAA AGACTGGCAT CACAGCTGCT AAGGAAAAGT TTGATGCCAC CATGAAAACC 
CTCACTATTG ACTGCGTCCA GTTTCAGAGA GGAGGCAAAG AATTCCTGAA GAAGCAAAAG 
CTGAGCCCTG ACGCAGTTGC CCAGCTGGCA TTCCAGATGG CCTTCCTGCG GCAGTACGGG 
CAGACAGTGG CCACCTACGA GTCCTGTAGC ACTGCCGCAT TCAAGCACGG CCGCACTGAG 
ACCATCCGCC CGGCCTCCGT CTATACAAAG AGGTGCTCTG AGGCCTTTGT CAGGGAGCCC 
TCCAGGCACA GTGCTGGTGA GCTTCAGCAG ATGATGGTTG AGTGCTCCAA GTACCATGGC 
CAGCTGACCA AAGAAGCAGC AATGGGCCAG GGCTTTGACC GACACTTGTT TGCTCTGCGG 
CATCTGGCAG CAGCCAAAGG GATCATCTTG CCTGAGCTCT ACCTGGACCC TGCATACGGG 
CAGATAAACC ACAATGTCCT GTCCACGAGC ACACTGAGCA GCCCAGCAGT GAACCTTGGG 
GGCTTTGCCC CTGTGGTCTC TGATGGCTTT GGTGTTGGGT ATGCTGTTCA TGACAACTGG 
ATAGGCTGCA ATGTCTCTTC CTACCCAGGC CGCAATGCCC GGGAGTTTCT CCAATGTGTG 
GAGAAGGCCT TAGAAGACAT GTTTGATGCC TTAGAAGGCA AATCCATCAA AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPT2 ( NM_000098.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000089.1 Homo sapiens carnitine palmitoyltransferase 2 (CPT2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17939
Clone ID Related Accession (Same CDS sequence)	NM_000098.3 , NM_000098.2
Accession Version	NM_000098.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	carnitine O-palmitoyltransferase 2, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U09648.1, AL606760.9 and BU629852.1. This sequence is a reference standard in the RefSeqGene project. On Mar 12, 2008 this sequence version replaced NM_000098.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005553.1, SRR1803612.118722.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCCCC GCCTGCTGCT GCGCGCCTGG CCCCGGGGCC CCGCGGTTGG TCCGGGAGCC 
CCCAGTCGGC CCCTCAGCGC CGGCTCCGGG CCCGGCCAGT ACCTGCAGCG CAGCATCGTG 
CCCACCATGC ACTACCAGGA CAGCCTGCCC AGGCTGCCTA TTCCCAAACT TGAAGACACC 
ATTAGGAGAT ACCTCAGTGC ACAGAAGCCT CTCTTGAATG ATGGCCAGTT CAGGAAAACA 
GAACAATTTT GCAAGAGTTT TGAAAATGGG ATTGGAAAAG AACTGCATGA GCAGCTGGTT 
GCTCTGGACA AACAGAATAA ACATACAAGC TACATTTCGG GACCCTGGTT TGATATGTAC 
CTATCTGCTC GAGACTCCGT TGTTCTGAAC TTTAATCCAT TTATGGCTTT CAATCCTGAC 
CCAAAATCTG AGTATAATGA CCAGCTCACC CGGGCAACCA ACATGACTGT TTCTGCCATC 
CGGTTTCTGA AGACACTCCG GGCTGGCCTT CTGGAGCCAG AAGTGTTCCA CTTGAACCCT 
GCAAAAAGTG ACACTATCAC CTTCAAGAGA CTCATACGCT TTGTGCCTTC CTCTCTGTCC 
TGGTATGGGG CCTACCTGGT CAATGCGTAT CCCCTGGATA TGTCCCAGTA TTTTCGGCTT 
TTCAACTCAA CTCGTTTACC CAAACCCAGT CGGGATGAAC TCTTCACTGA TGACAAGGCC 
AGACACCTCC TGGTCCTAAG GAAAGGAAAT TTTTATATCT TTGATGTCCT GGATCAAGAT 
GGGAACATTG TGAGCCCCTC GGAAATCCAG GCACATCTGA AGTACATTCT CTCAGACAGC 
AGCCCCGCCC CCGAGTTTCC CCTGGCATAC CTGACCAGTG AGAACCGAGA CATCTGGGCA 
GAGCTCAGGC AGAAGCTGAT GAGTAGTGGC AATGAGGAGA GCCTGAGGAA AGTGGACTCG 
GCAGTGTTCT GTCTCTGCCT AGATGACTTC CCCATTAAGG ACCTTGTCCA CTTGTCCCAC 
AATATGCTGC ATGGGGATGG CACAAACCGC TGGTTTGATA AATCCTTTAA CCTCATTATC 
GCCAAGGATG GCTCTACTGC CGTCCACTTT GAGCACTCTT GGGGTGATGG TGTGGCAGTG 
CTCAGATTTT TTAATGAAGT ATTTAAAGAC AGCACTCAGA CCCCTGCCGT CACTCCACAG 
AGCCAGCCAG CTACCACTGA CTCTACTGTC ACGGTGCAGA AACTCAACTT CGAGCTGACT 
GATGCCTTAA AGACTGGCAT CACAGCTGCT AAGGAAAAGT TTGATGCCAC CATGAAAACC 
CTCACTATTG ACTGCGTCCA GTTTCAGAGA GGAGGCAAAG AATTCCTGAA GAAGCAAAAG 
CTGAGCCCTG ACGCAGTTGC CCAGCTGGCA TTCCAGATGG CCTTCCTGCG GCAGTACGGG 
CAGACAGTGG CCACCTACGA GTCCTGTAGC ACTGCCGCAT TCAAGCACGG CCGCACTGAG 
ACCATCCGCC CGGCCTCCGT CTATACAAAG AGGTGCTCTG AGGCCTTTGT CAGGGAGCCC 
TCCAGGCACA GTGCTGGTGA GCTTCAGCAG ATGATGGTTG AGTGCTCCAA GTACCATGGC 
CAGCTGACCA AAGAAGCAGC AATGGGCCAG GGCTTTGACC GACACTTGTT TGCTCTGCGG 
CATCTGGCAG CAGCCAAAGG GATCATCTTG CCTGAGCTCT ACCTGGACCC TGCATACGGG 
CAGATAAACC ACAATGTCCT GTCCACGAGC ACACTGAGCA GCCCAGCAGT GAACCTTGGG 
GGCTTTGCCC CTGTGGTCTC TGATGGCTTT GGTGTTGGGT ATGCTGTTCA TGACAACTGG 
ATAGGCTGCA ATGTCTCTTC CTACCCAGGC CGCAATGCCC GGGAGTTTCT CCAATGTGTG 
GAGAAGGCCT TAGAAGACAT GTTTGATGCC TTAGAAGGCA AATCCATCAA AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000089.1
CDS	516..2492
Translation	MVPRLLLRAWPRGPAVGPGAPSRPLSAGSGPGQYLQRSIVPTMHYQDSLPRLPIPKLEDTIRRYLSAQKPLLNDGQFRKTEQFCKSFENGIGKELHEQLVALDKQNKHTSYISGPWFDMYLSARDSVVLNFNPFMAFNPDPKSEYNDQLTRATNMTVSAIRFLKTLRAGLLEPEVFHLNPAKSDTITFKRLIRFVPSSLSWYGAYLVNAYPLDMSQYFRLFNSTRLPKPSRDELFTDDKARHLLVLRKGNFYIFDVLDQDGNIVSPSEIQAHLKYILSDSSPAPEFPLAYLTSENRDIWAELRQKLMSSGNEESLRKVDSAVFCLCLDDFPIKDLVHLSHNMLHGDGTNRWFDKSFNLIIAKDGSTAVHFEHSWGDGVAVLRFFNEVFKDSTQTPAVTPQSQPATTDSTVTVQKLNFELTDALKTGITAAKEKFDATMKTLTIDCVQFQRGGKEFLKKQKLSPDAVAQLAFQMAFLRQYGQTVATYESCSTAAFKHGRTETIRPASVYTKRCSEAFVREPSRHSAGELQQMMVECSKYHGQLTKEAAMGQGFDRHLFALRHLAAAKGIILPELYLDPAYGQINHNVLSTSTLSSPAVNLGGFAPVVSDGFGVGYAVHDNWIGCNVSSYPGRNAREFLQCVEKALEDMFDALEGKSIKS

Target ORF information:

RefSeq Version	NM_000098.2
Organism	Homo sapiens(human)
Definition	Homo sapiens carnitine palmitoyltransferase 2 (CPT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000098.2

ORF Insert Sequence:

ATGGTGCCCC GCCTGCTGCT GCGCGCCTGG CCCCGGGGCC CCGCGGTTGG TCCGGGAGCC 
CCCAGTCGGC CCCTCAGCGC CGGCTCCGGG CCCGGCCAGT ACCTGCAGCG CAGCATCGTG 
CCCACCATGC ACTACCAGGA CAGCCTGCCC AGGCTGCCTA TTCCCAAACT TGAAGACACC 
ATTAGGAGAT ACCTCAGTGC ACAGAAGCCT CTCTTGAATG ATGGCCAGTT CAGGAAAACA 
GAACAATTTT GCAAGAGTTT TGAAAATGGG ATTGGAAAAG AACTGCATGA GCAGCTGGTT 
GCTCTGGACA AACAGAATAA ACATACAAGC TACATTTCGG GACCCTGGTT TGATATGTAC 
CTATCTGCTC GAGACTCCGT TGTTCTGAAC TTTAATCCAT TTATGGCTTT CAATCCTGAC 
CCAAAATCTG AGTATAATGA CCAGCTCACC CGGGCAACCA ACATGACTGT TTCTGCCATC 
CGGTTTCTGA AGACACTCCG GGCTGGCCTT CTGGAGCCAG AAGTGTTCCA CTTGAACCCT 
GCAAAAAGTG ACACTATCAC CTTCAAGAGA CTCATACGCT TTGTGCCTTC CTCTCTGTCC 
TGGTATGGGG CCTACCTGGT CAATGCGTAT CCCCTGGATA TGTCCCAGTA TTTTCGGCTT 
TTCAACTCAA CTCGTTTACC CAAACCCAGT CGGGATGAAC TCTTCACTGA TGACAAGGCC 
AGACACCTCC TGGTCCTAAG GAAAGGAAAT TTTTATATCT TTGATGTCCT GGATCAAGAT 
GGGAACATTG TGAGCCCCTC GGAAATCCAG GCACATCTGA AGTACATTCT CTCAGACAGC 
AGCCCCGCCC CCGAGTTTCC CCTGGCATAC CTGACCAGTG AGAACCGAGA CATCTGGGCA 
GAGCTCAGGC AGAAGCTGAT GAGTAGTGGC AATGAGGAGA GCCTGAGGAA AGTGGACTCG 
GCAGTGTTCT GTCTCTGCCT AGATGACTTC CCCATTAAGG ACCTTGTCCA CTTGTCCCAC 
AATATGCTGC ATGGGGATGG CACAAACCGC TGGTTTGATA AATCCTTTAA CCTCATTATC 
GCCAAGGATG GCTCTACTGC CGTCCACTTT GAGCACTCTT GGGGTGATGG TGTGGCAGTG 
CTCAGATTTT TTAATGAAGT ATTTAAAGAC AGCACTCAGA CCCCTGCCGT CACTCCACAG 
AGCCAGCCAG CTACCACTGA CTCTACTGTC ACGGTGCAGA AACTCAACTT CGAGCTGACT 
GATGCCTTAA AGACTGGCAT CACAGCTGCT AAGGAAAAGT TTGATGCCAC CATGAAAACC 
CTCACTATTG ACTGCGTCCA GTTTCAGAGA GGAGGCAAAG AATTCCTGAA GAAGCAAAAG 
CTGAGCCCTG ACGCAGTTGC CCAGCTGGCA TTCCAGATGG CCTTCCTGCG GCAGTACGGG 
CAGACAGTGG CCACCTACGA GTCCTGTAGC ACTGCCGCAT TCAAGCACGG CCGCACTGAG 
ACCATCCGCC CGGCCTCCGT CTATACAAAG AGGTGCTCTG AGGCCTTTGT CAGGGAGCCC 
TCCAGGCACA GTGCTGGTGA GCTTCAGCAG ATGATGGTTG AGTGCTCCAA GTACCATGGC 
CAGCTGACCA AAGAAGCAGC AATGGGCCAG GGCTTTGACC GACACTTGTT TGCTCTGCGG 
CATCTGGCAG CAGCCAAAGG GATCATCTTG CCTGAGCTCT ACCTGGACCC TGCATACGGG 
CAGATAAACC ACAATGTCCT GTCCACGAGC ACACTGAGCA GCCCAGCAGT GAACCTTGGG 
GGCTTTGCCC CTGTGGTCTC TGATGGCTTT GGTGTTGGGT ATGCTGTTCA TGACAACTGG 
ATAGGCTGCA ATGTCTCTTC CTACCCAGGC CGCAATGCCC GGGAGTTTCT CCAATGTGTG 
GAGAAGGCCT TAGAAGACAT GTTTGATGCC TTAGAAGGCA AATCCATCAA AAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPT2 ( NM_001330589.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317518.1 Homo sapiens carnitine palmitoyltransferase 2 (CPT2), transcript variant 2, mRNA.

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CloneID	OHu33029
Clone ID Related Accession (Same CDS sequence)	NM_001330589.1 , NM_001330589.2
Accession Version	NM_001330589.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	carnitine O-palmitoyltransferase 2, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL606760.9. On Aug 29, 2016 this sequence version replaced XM_005270484.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.95817.1, SRR1803616.236243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCCCC GCCTGCTGCT GCGCGCCTGG CCCCGGGGCC CCGCGGTTGG TCCGGGAGCC 
CCCAGTCGGC CCCTCAGCGC CGGCTCCGGG CCCGGCCAGT ACCTGCAGCG CAGCATCGTG 
CCCACCATGC ACTACCAGGA CAGCCTGCCC AGGCTGCCTA TTCCCAAACT TGAAGACACC 
ATTAGGAGAT ACCTCAGTGC ACAGAAGCCT CTCTTGAATG ATGGCCAGTT CAGGAAAACA 
GAACAATTTT GCAAGAGTTT TGAAAATGGG ATTGGAAAAG AACTGCATGA GCAGCTGGTT 
GCTCTGGACA AACAGAATAA ACATACAAGC TACATTTCGG GACCCTGGTT TGATATGTAC 
CTATCTGCTC GAGACTCCGT TGTTCTGAAC TTTAATCCAT TTATGGCTTT CAATCCTGAC 
CCAAAATCTG AGTATAATGA CCAGCTCACC CGGGCAACCA ACATGACTGT TTCTGCCATC 
CGGTTTCTGA AGACACTCCG GGCTGGCCTT CTGGAGCCAG AAGTGTTCCA CTTGAACCCT 
GCAAAAAGTG ACACTATCAC CTTCAAGAGA CTCATACGCT TTGTGCCTTC CTCTCTGTCC 
TGGTATGGGG CCTACCTGGT CAATGCGTAT CCCCTGGATA TGTCCCAGTA TTTTCGGCTT 
TTCAACTCAA CTCGTTTACC CAAACCCAGT CGGGATGAAC TCTTCACTGA TGACAAGGCC 
AGACACCTCC TGGTCCTAAG GAAAGGAAAT TTTTATATCT TTGATGTCCT GGATCAAGAT 
GGGAACATTG TGAGCCCCTC GGAAATCCAG GCACATCTGA AGTACATTCT CTCAGACAGC 
AGCCCCGCCC CCGAGTTTCC CCTGGCATAC CTGACCAGTG AGAACCGAGA CATCTGGGCA 
GAGCTCAGGC AGAAGCTGAT GAGTAGTGGC AATGAGGAGA GCCTGAGGAA AGTGGACTCG 
GCAGTGTTCT GTCTCTGCCT AGATGACTTC CCCATTAAGG ACCTTGTCCA CTTGTCCCAC 
AATATGCTGC ATGGGGATGG CACAAACCGC TGGTTTGATA AATCCTTTAA CCTCATTATC 
GCCAAGGATG GCTCTACTGC CGTCCACTTT GAGCACTCTT GGGGTGATGG TGTGGCAGTG 
CTCAGATTTT TTAATGAAGT ATTTAAAGAC AGCACTCAGA CCCCTGCCGT CACTCCACAG 
AGCCAGCCAG CTACCACTGA CTCTACTGTC ACGGTGCAGA AACTCAACTT CGAGCTGACT 
GATGCCTTAA AGACTGGCAT CACAGCTGCT AAGGAAAAGT TTGATGCCAC CATGAAAACC 
CTCACTATTG ACTGCGTCCA GTTTCAGAGA GGAGGCAAAG AATTCCTGAA GAAGCAAAAG 
CTGAGCCCTG ACGCAGTTGC CCAGCTGGCA TTCCAGATGG CCTTCCTGCG GCAGTACGGG 
CAGACAGTGG CCACCTACGA GTCCTGTAGC ACTGCCGCAT TCAAGCACGG CCGCACTGAG 
ACCATCCGCC CGGCCTCCGT CTATACAAAG AGGTGCTCTG AGGCCTTTGT CAGGGAGCCC 
TCCAGGCACA GTGCTGGCCA GGGCTTTGAC CGACACTTGT TTGCTCTGCG GCATCTGGCA 
GCAGCCAAAG GGATCATCTT GCCTGAGCTC TACCTGGACC CTGCATACGG GCAGATAAAC 
CACAATGTCC TGTCCACGAG CACACTGAGC AGCCCAGCAG TGAACCTTGG GGGCTTTGCC 
CCTGTGGTCT CTGATGGCTT TGGTGTTGGG TATGCTGTTC ATGACAACTG GATAGGCTGC 
AATGTCTCTT CCTACCCAGG CCGCAATGCC CGGGAGTTTC TCCAATGTGT GGAGAAGGCC 
TTAGAAGACA TGTTTGATGC CTTAGAAGGC AAATCCATCA AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317518.1
CDS	516..2423
Translation	MVPRLLLRAWPRGPAVGPGAPSRPLSAGSGPGQYLQRSIVPTMHYQDSLPRLPIPKLEDTIRRYLSAQKPLLNDGQFRKTEQFCKSFENGIGKELHEQLVALDKQNKHTSYISGPWFDMYLSARDSVVLNFNPFMAFNPDPKSEYNDQLTRATNMTVSAIRFLKTLRAGLLEPEVFHLNPAKSDTITFKRLIRFVPSSLSWYGAYLVNAYPLDMSQYFRLFNSTRLPKPSRDELFTDDKARHLLVLRKGNFYIFDVLDQDGNIVSPSEIQAHLKYILSDSSPAPEFPLAYLTSENRDIWAELRQKLMSSGNEESLRKVDSAVFCLCLDDFPIKDLVHLSHNMLHGDGTNRWFDKSFNLIIAKDGSTAVHFEHSWGDGVAVLRFFNEVFKDSTQTPAVTPQSQPATTDSTVTVQKLNFELTDALKTGITAAKEKFDATMKTLTIDCVQFQRGGKEFLKKQKLSPDAVAQLAFQMAFLRQYGQTVATYESCSTAAFKHGRTETIRPASVYTKRCSEAFVREPSRHSAGQGFDRHLFALRHLAAAKGIILPELYLDPAYGQINHNVLSTSTLSSPAVNLGGFAPVVSDGFGVGYAVHDNWIGCNVSSYPGRNAREFLQCVEKALEDMFDALEGKSIKS

Target ORF information:

RefSeq Version	NM_001330589.1
Organism	Homo sapiens(human)
Definition	Homo sapiens carnitine palmitoyltransferase 2 (CPT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330589.1

ORF Insert Sequence:

ATGGTGCCCC GCCTGCTGCT GCGCGCCTGG CCCCGGGGCC CCGCGGTTGG TCCGGGAGCC 
CCCAGTCGGC CCCTCAGCGC CGGCTCCGGG CCCGGCCAGT ACCTGCAGCG CAGCATCGTG 
CCCACCATGC ACTACCAGGA CAGCCTGCCC AGGCTGCCTA TTCCCAAACT TGAAGACACC 
ATTAGGAGAT ACCTCAGTGC ACAGAAGCCT CTCTTGAATG ATGGCCAGTT CAGGAAAACA 
GAACAATTTT GCAAGAGTTT TGAAAATGGG ATTGGAAAAG AACTGCATGA GCAGCTGGTT 
GCTCTGGACA AACAGAATAA ACATACAAGC TACATTTCGG GACCCTGGTT TGATATGTAC 
CTATCTGCTC GAGACTCCGT TGTTCTGAAC TTTAATCCAT TTATGGCTTT CAATCCTGAC 
CCAAAATCTG AGTATAATGA CCAGCTCACC CGGGCAACCA ACATGACTGT TTCTGCCATC 
CGGTTTCTGA AGACACTCCG GGCTGGCCTT CTGGAGCCAG AAGTGTTCCA CTTGAACCCT 
GCAAAAAGTG ACACTATCAC CTTCAAGAGA CTCATACGCT TTGTGCCTTC CTCTCTGTCC 
TGGTATGGGG CCTACCTGGT CAATGCGTAT CCCCTGGATA TGTCCCAGTA TTTTCGGCTT 
TTCAACTCAA CTCGTTTACC CAAACCCAGT CGGGATGAAC TCTTCACTGA TGACAAGGCC 
AGACACCTCC TGGTCCTAAG GAAAGGAAAT TTTTATATCT TTGATGTCCT GGATCAAGAT 
GGGAACATTG TGAGCCCCTC GGAAATCCAG GCACATCTGA AGTACATTCT CTCAGACAGC 
AGCCCCGCCC CCGAGTTTCC CCTGGCATAC CTGACCAGTG AGAACCGAGA CATCTGGGCA 
GAGCTCAGGC AGAAGCTGAT GAGTAGTGGC AATGAGGAGA GCCTGAGGAA AGTGGACTCG 
GCAGTGTTCT GTCTCTGCCT AGATGACTTC CCCATTAAGG ACCTTGTCCA CTTGTCCCAC 
AATATGCTGC ATGGGGATGG CACAAACCGC TGGTTTGATA AATCCTTTAA CCTCATTATC 
GCCAAGGATG GCTCTACTGC CGTCCACTTT GAGCACTCTT GGGGTGATGG TGTGGCAGTG 
CTCAGATTTT TTAATGAAGT ATTTAAAGAC AGCACTCAGA CCCCTGCCGT CACTCCACAG 
AGCCAGCCAG CTACCACTGA CTCTACTGTC ACGGTGCAGA AACTCAACTT CGAGCTGACT 
GATGCCTTAA AGACTGGCAT CACAGCTGCT AAGGAAAAGT TTGATGCCAC CATGAAAACC 
CTCACTATTG ACTGCGTCCA GTTTCAGAGA GGAGGCAAAG AATTCCTGAA GAAGCAAAAG 
CTGAGCCCTG ACGCAGTTGC CCAGCTGGCA TTCCAGATGG CCTTCCTGCG GCAGTACGGG 
CAGACAGTGG CCACCTACGA GTCCTGTAGC ACTGCCGCAT TCAAGCACGG CCGCACTGAG 
ACCATCCGCC CGGCCTCCGT CTATACAAAG AGGTGCTCTG AGGCCTTTGT CAGGGAGCCC 
TCCAGGCACA GTGCTGGCCA GGGCTTTGAC CGACACTTGT TTGCTCTGCG GCATCTGGCA 
GCAGCCAAAG GGATCATCTT GCCTGAGCTC TACCTGGACC CTGCATACGG GCAGATAAAC 
CACAATGTCC TGTCCACGAG CACACTGAGC AGCCCAGCAG TGAACCTTGG GGGCTTTGCC 
CCTGTGGTCT CTGATGGCTT TGGTGTTGGG TATGCTGTTC ATGACAACTG GATAGGCTGC 
AATGTCTCTT CCTACCCAGG CCGCAATGCC CGGGAGTTTC TCCAATGTGT GGAGAAGGCC 
TTAGAAGACA TGTTTGATGC CTTAGAAGGC AAATCCATCA AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPT2 ( NM_001330589.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317518.1 Homo sapiens carnitine palmitoyltransferase 2 (CPT2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu33029
Clone ID Related Accession (Same CDS sequence)	NM_001330589.1 , NM_001330589.2
Accession Version	NM_001330589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	carnitine O-palmitoyltransferase 2, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL606760.9. On May 31, 2019 this sequence version replaced NM_001330589.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.82312.1, SRR1660805.10146.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCCCC GCCTGCTGCT GCGCGCCTGG CCCCGGGGCC CCGCGGTTGG TCCGGGAGCC 
CCCAGTCGGC CCCTCAGCGC CGGCTCCGGG CCCGGCCAGT ACCTGCAGCG CAGCATCGTG 
CCCACCATGC ACTACCAGGA CAGCCTGCCC AGGCTGCCTA TTCCCAAACT TGAAGACACC 
ATTAGGAGAT ACCTCAGTGC ACAGAAGCCT CTCTTGAATG ATGGCCAGTT CAGGAAAACA 
GAACAATTTT GCAAGAGTTT TGAAAATGGG ATTGGAAAAG AACTGCATGA GCAGCTGGTT 
GCTCTGGACA AACAGAATAA ACATACAAGC TACATTTCGG GACCCTGGTT TGATATGTAC 
CTATCTGCTC GAGACTCCGT TGTTCTGAAC TTTAATCCAT TTATGGCTTT CAATCCTGAC 
CCAAAATCTG AGTATAATGA CCAGCTCACC CGGGCAACCA ACATGACTGT TTCTGCCATC 
CGGTTTCTGA AGACACTCCG GGCTGGCCTT CTGGAGCCAG AAGTGTTCCA CTTGAACCCT 
GCAAAAAGTG ACACTATCAC CTTCAAGAGA CTCATACGCT TTGTGCCTTC CTCTCTGTCC 
TGGTATGGGG CCTACCTGGT CAATGCGTAT CCCCTGGATA TGTCCCAGTA TTTTCGGCTT 
TTCAACTCAA CTCGTTTACC CAAACCCAGT CGGGATGAAC TCTTCACTGA TGACAAGGCC 
AGACACCTCC TGGTCCTAAG GAAAGGAAAT TTTTATATCT TTGATGTCCT GGATCAAGAT 
GGGAACATTG TGAGCCCCTC GGAAATCCAG GCACATCTGA AGTACATTCT CTCAGACAGC 
AGCCCCGCCC CCGAGTTTCC CCTGGCATAC CTGACCAGTG AGAACCGAGA CATCTGGGCA 
GAGCTCAGGC AGAAGCTGAT GAGTAGTGGC AATGAGGAGA GCCTGAGGAA AGTGGACTCG 
GCAGTGTTCT GTCTCTGCCT AGATGACTTC CCCATTAAGG ACCTTGTCCA CTTGTCCCAC 
AATATGCTGC ATGGGGATGG CACAAACCGC TGGTTTGATA AATCCTTTAA CCTCATTATC 
GCCAAGGATG GCTCTACTGC CGTCCACTTT GAGCACTCTT GGGGTGATGG TGTGGCAGTG 
CTCAGATTTT TTAATGAAGT ATTTAAAGAC AGCACTCAGA CCCCTGCCGT CACTCCACAG 
AGCCAGCCAG CTACCACTGA CTCTACTGTC ACGGTGCAGA AACTCAACTT CGAGCTGACT 
GATGCCTTAA AGACTGGCAT CACAGCTGCT AAGGAAAAGT TTGATGCCAC CATGAAAACC 
CTCACTATTG ACTGCGTCCA GTTTCAGAGA GGAGGCAAAG AATTCCTGAA GAAGCAAAAG 
CTGAGCCCTG ACGCAGTTGC CCAGCTGGCA TTCCAGATGG CCTTCCTGCG GCAGTACGGG 
CAGACAGTGG CCACCTACGA GTCCTGTAGC ACTGCCGCAT TCAAGCACGG CCGCACTGAG 
ACCATCCGCC CGGCCTCCGT CTATACAAAG AGGTGCTCTG AGGCCTTTGT CAGGGAGCCC 
TCCAGGCACA GTGCTGGCCA GGGCTTTGAC CGACACTTGT TTGCTCTGCG GCATCTGGCA 
GCAGCCAAAG GGATCATCTT GCCTGAGCTC TACCTGGACC CTGCATACGG GCAGATAAAC 
CACAATGTCC TGTCCACGAG CACACTGAGC AGCCCAGCAG TGAACCTTGG GGGCTTTGCC 
CCTGTGGTCT CTGATGGCTT TGGTGTTGGG TATGCTGTTC ATGACAACTG GATAGGCTGC 
AATGTCTCTT CCTACCCAGG CCGCAATGCC CGGGAGTTTC TCCAATGTGT GGAGAAGGCC 
TTAGAAGACA TGTTTGATGC CTTAGAAGGC AAATCCATCA AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317518.1
CDS	121..2028
Translation	MVPRLLLRAWPRGPAVGPGAPSRPLSAGSGPGQYLQRSIVPTMHYQDSLPRLPIPKLEDTIRRYLSAQKPLLNDGQFRKTEQFCKSFENGIGKELHEQLVALDKQNKHTSYISGPWFDMYLSARDSVVLNFNPFMAFNPDPKSEYNDQLTRATNMTVSAIRFLKTLRAGLLEPEVFHLNPAKSDTITFKRLIRFVPSSLSWYGAYLVNAYPLDMSQYFRLFNSTRLPKPSRDELFTDDKARHLLVLRKGNFYIFDVLDQDGNIVSPSEIQAHLKYILSDSSPAPEFPLAYLTSENRDIWAELRQKLMSSGNEESLRKVDSAVFCLCLDDFPIKDLVHLSHNMLHGDGTNRWFDKSFNLIIAKDGSTAVHFEHSWGDGVAVLRFFNEVFKDSTQTPAVTPQSQPATTDSTVTVQKLNFELTDALKTGITAAKEKFDATMKTLTIDCVQFQRGGKEFLKKQKLSPDAVAQLAFQMAFLRQYGQTVATYESCSTAAFKHGRTETIRPASVYTKRCSEAFVREPSRHSAGQGFDRHLFALRHLAAAKGIILPELYLDPAYGQINHNVLSTSTLSSPAVNLGGFAPVVSDGFGVGYAVHDNWIGCNVSSYPGRNAREFLQCVEKALEDMFDALEGKSIKS

Target ORF information:

RefSeq Version	NM_001330589.2
Organism	Homo sapiens(human)
Definition	Homo sapiens carnitine palmitoyltransferase 2 (CPT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330589.2

ORF Insert Sequence:

ATGGTGCCCC GCCTGCTGCT GCGCGCCTGG CCCCGGGGCC CCGCGGTTGG TCCGGGAGCC 
CCCAGTCGGC CCCTCAGCGC CGGCTCCGGG CCCGGCCAGT ACCTGCAGCG CAGCATCGTG 
CCCACCATGC ACTACCAGGA CAGCCTGCCC AGGCTGCCTA TTCCCAAACT TGAAGACACC 
ATTAGGAGAT ACCTCAGTGC ACAGAAGCCT CTCTTGAATG ATGGCCAGTT CAGGAAAACA 
GAACAATTTT GCAAGAGTTT TGAAAATGGG ATTGGAAAAG AACTGCATGA GCAGCTGGTT 
GCTCTGGACA AACAGAATAA ACATACAAGC TACATTTCGG GACCCTGGTT TGATATGTAC 
CTATCTGCTC GAGACTCCGT TGTTCTGAAC TTTAATCCAT TTATGGCTTT CAATCCTGAC 
CCAAAATCTG AGTATAATGA CCAGCTCACC CGGGCAACCA ACATGACTGT TTCTGCCATC 
CGGTTTCTGA AGACACTCCG GGCTGGCCTT CTGGAGCCAG AAGTGTTCCA CTTGAACCCT 
GCAAAAAGTG ACACTATCAC CTTCAAGAGA CTCATACGCT TTGTGCCTTC CTCTCTGTCC 
TGGTATGGGG CCTACCTGGT CAATGCGTAT CCCCTGGATA TGTCCCAGTA TTTTCGGCTT 
TTCAACTCAA CTCGTTTACC CAAACCCAGT CGGGATGAAC TCTTCACTGA TGACAAGGCC 
AGACACCTCC TGGTCCTAAG GAAAGGAAAT TTTTATATCT TTGATGTCCT GGATCAAGAT 
GGGAACATTG TGAGCCCCTC GGAAATCCAG GCACATCTGA AGTACATTCT CTCAGACAGC 
AGCCCCGCCC CCGAGTTTCC CCTGGCATAC CTGACCAGTG AGAACCGAGA CATCTGGGCA 
GAGCTCAGGC AGAAGCTGAT GAGTAGTGGC AATGAGGAGA GCCTGAGGAA AGTGGACTCG 
GCAGTGTTCT GTCTCTGCCT AGATGACTTC CCCATTAAGG ACCTTGTCCA CTTGTCCCAC 
AATATGCTGC ATGGGGATGG CACAAACCGC TGGTTTGATA AATCCTTTAA CCTCATTATC 
GCCAAGGATG GCTCTACTGC CGTCCACTTT GAGCACTCTT GGGGTGATGG TGTGGCAGTG 
CTCAGATTTT TTAATGAAGT ATTTAAAGAC AGCACTCAGA CCCCTGCCGT CACTCCACAG 
AGCCAGCCAG CTACCACTGA CTCTACTGTC ACGGTGCAGA AACTCAACTT CGAGCTGACT 
GATGCCTTAA AGACTGGCAT CACAGCTGCT AAGGAAAAGT TTGATGCCAC CATGAAAACC 
CTCACTATTG ACTGCGTCCA GTTTCAGAGA GGAGGCAAAG AATTCCTGAA GAAGCAAAAG 
CTGAGCCCTG ACGCAGTTGC CCAGCTGGCA TTCCAGATGG CCTTCCTGCG GCAGTACGGG 
CAGACAGTGG CCACCTACGA GTCCTGTAGC ACTGCCGCAT TCAAGCACGG CCGCACTGAG 
ACCATCCGCC CGGCCTCCGT CTATACAAAG AGGTGCTCTG AGGCCTTTGT CAGGGAGCCC 
TCCAGGCACA GTGCTGGCCA GGGCTTTGAC CGACACTTGT TTGCTCTGCG GCATCTGGCA 
GCAGCCAAAG GGATCATCTT GCCTGAGCTC TACCTGGACC CTGCATACGG GCAGATAAAC 
CACAATGTCC TGTCCACGAG CACACTGAGC AGCCCAGCAG TGAACCTTGG GGGCTTTGCC 
CCTGTGGTCT CTGATGGCTT TGGTGTTGGG TATGCTGTTC ATGACAACTG GATAGGCTGC 
AATGTCTCTT CCTACCCAGG CCGCAATGCC CGGGAGTTTC TCCAATGTGT GGAGAAGGCC 
TTAGAAGACA TGTTTGATGC CTTAGAAGGC AAATCCATCA AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Muscle Carnitine Palmitoyltransferase II Deficiency: A Review of Enzymatic Controversy and Clinical Features.
International journal of molecular sciences18(1)(2017 Jan)
Lehmann D,Motlagh L,Robaa D,Zierz S

Genetic evaluation of AMPD1, CPT2, and PGYM metabolic enzymes in patients with chronic fatigue syndrome.
Genetics and molecular research : GMR15(3)(2016 Jul)
Maltese PE,Venturini L,Poplavskaya E,Bertelli M,Cecchin S,Granato M,Nikulina SY,Salmina A,Aksyutina N,Capelli E,Ricevuti G,Lorusso L

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CPT2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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