CPS1 cDNA ORF clone, Homo sapiens(human)

The following CPS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CPS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu24775 NM_001122633.2
Latest version!
Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu24805 NM_001122633.3
Latest version!
Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu24805 NM_001875.5
Latest version!
Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu24805 NM_001369257.1
Latest version!
Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu25055 NM_001122634.3
Latest version!
Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu24805 XM_011510641.2
Latest version!
Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu24805 XM_011510644.2
Latest version!
Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu24805 XM_011510643.2
Latest version!
Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu24805 XM_011510642.2
Latest version!
Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu57055 NM_001369256.1
Latest version!
Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu57055 XM_011510640.1
Latest version!
Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
View Old Accession Versions >>
OHu24805 XM_011510643.1 Homo sapiens carbamoyl-phosphate synthase 1, mitochondrial (CPS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu24805 XM_011510644.1 Homo sapiens carbamoyl-phosphate synthase 1, mitochondrial (CPS1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu24805 XM_011510642.1 Homo sapiens carbamoyl-phosphate synthase 1, mitochondrial (CPS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu24805 NM_001875.4 Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu24805 XM_011510641.1 Homo sapiens carbamoyl-phosphate synthase 1, mitochondrial (CPS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu24775
    Clone ID Related Accession (Same CDS sequence) NM_001122633.2
    Accession Version NM_001122633.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA804480.1, AB208800.1, Y15793.1 and BC058010.1. On Apr 6, 2011 this sequence version replaced NM_001122633.1. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB208800.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCACAAA TCATCAAAAT GACGAGGATT TTGACAGCTT TCAAAGTGGT GAGGACACTG 
    AAGACTGGTT TTGGCTTTAC CAATGTGACT GCACACCAAA AATGGAAATT TTCAAGACCT
    GGCATCAGGC TCCTTTCTGT CAAGGCACAG ACAGCACACA TTGTCCTGGA AGATGGAACT
    AAGATGAAAG GTTACTCCTT TGGCCATCCA TCCTCTGTTG CTGGTGAAGT GGTTTTTAAT
    ACTGGCCTGG GAGGGTACCC AGAAGCTATT ACTGACCCTG CCTACAAAGG ACAGATTCTC
    ACAATGGCCA ACCCTATTAT TGGGAATGGT GGAGCTCCTG ATACTACTGC TCTGGATGAA
    CTGGGACTTA GCAAATATTT GGAGTCTAAT GGAATCAAGG TTTCAGGTTT GCTGGTGCTG
    GATTATAGTA AAGACTACAA CCACTGGCTG GCTACCAAGA GTTTAGGGCA ATGGCTACAG
    GAAGAAAAGG TTCCTGCAAT TTATGGAGTG GACACAAGAA TGCTGACTAA AATAATTCGG
    GATAAGGGTA CCATGCTTGG GAAGATTGAA TTTGAAGGTC AGCCTGTGGA TTTTGTGGAT
    CCAAATAAAC AGAATTTGAT TGCTGAGGTT TCAACCAAGG ATGTCAAAGT GTACGGCAAA
    GGAAACCCCA CAAAAGTGGT AGCTGTAGAC TGTGGGATTA AAAACAATGT AATCCGCCTG
    CTAGTAAAGC GAGGAGCTGA AGTGCACTTA GTTCCCTGGA ACCATGATTT CACCAAGATG
    GAGTATGATG GGATTTTGAT CGCGGGAGGA CCGGGGAACC CAGCTCTTGC AGAACCACTA
    ATTCAGAATG TCAGAAAGAT TTTGGAGAGT GATCGCAAGG AGCCATTGTT TGGAATCAGT
    ACAGGAAACT TAATAACAGG ATTGGCTGCT GGTGCCAAAA CCTACAAGAT GTCCATGGCC
    AACAGAGGGC AGAATCAGCC TGTTTTGAAT ATCACAAACA AACAGGCTTT CATTACTGCT
    CAGAATCATG GCTATGCCTT GGACAACACC CTCCCTGCTG GCTGGAAACC ACTTTTTGTG
    AATGTCAACG ATCAAACAAA TGAGGGGATT ATGCATGAGA GCAAACCCTT CTTCGCTGTG
    CAGTTCCACC CAGAGGTCAC CCCGGGGCCA ATAGACACTG AGTACCTGTT TGATTCCTTT
    TTCTCACTGA TAAAGAAAGG AAAAGCTACC ACCATTACAT CAGTCTTACC GAAGCCAGCA
    CTAGTTGCAT CTCGGGTTGA GGTTTCCAAA GTCCTTATTC TAGGATCAGG AGGTCTGTCC
    ATTGGTCAGG CTGGAGAATT TGATTACTCA GGATCTCAAG CTGTAAAAGC CATGAAGGAA
    GAAAATGTCA AAACTGTTCT GATGAACCCA AACATTGCAT CAGTCCAGAC CAATGAGGTG
    GGCTTAAAGC AAGCGGATAC TGTCTACTTT CTTCCCATCA CCCCTCAGTT TGTCACAGAG
    GTCATCAAGG CAGAACAGCC AGATGGGTTA ATTCTGGGCA TGGGTGGCCA GACAGCTCTG
    AACTGTGGAG TGGAACTATT CAAGAGAGGT GTGCTCAAGG AATATGGTGT GAAAGTCCTG
    GGAACTTCAG TTGAGTCCAT TATGGCTACG GAAGACAGGC AGCTGTTTTC AGATAAACTA
    AATGAGATCA ATGAAAAGAT TGCTCCAAGT TTTGCAGTGG AATCGATTGA GGATGCACTG
    AAGGCAGCAG ACACCATTGG CTACCCAGTG ATGATCCGTT CCGCCTATGC ACTGGGTGGG
    TTAGGCTCAG GCATCTGTCC CAACAGAGAG ACTTTGATGG ACCTCAGCAC AAAGGCCTTT
    GCTATGACCA ACCAAATTCT GGTGGAGAAG TCAGTGACAG GTTGGAAAGA AATAGAATAT
    GAAGTGGTTC GAGATGCTGA TGACAATTGT GTCACTGTCT GTAACATGGA AAATGTTGAT
    GCCATGGGTG TTCACACAGG TGACTCAGTT GTTGTGGCTC CTGCCCAGAC ACTCTCCAAT
    GCCGAGTTTC AGATGTTGAG ACGTACTTCA ATCAATGTTG TTCGCCACTT GGGCATTGTG
    GGTGAATGCA ACATTCAGTT TGCCCTTCAT CCTACCTCAA TGGAATACTG CATCATTGAA
    GTGAATGCCA GACTGTCCCG AAGCTCTGCT CTGGCCTCAA AAGCCACTGG CTACCCATTG
    GCATTCATTG CTGCAAAGAT TGCCCTAGGA ATCCCACTTC CAGAAATTAA GAACGTCGTA
    TCCGGGAAGA CATCAGCCTG TTTTGAACCT AGCCTGGATT ACATGGTCAC CAAGATTCCC
    CGCTGGGATC TTGACCGTTT TCATGGAACA TCTAGCCGAA TTGGTAGCTC TATGAAAAGT
    GTAGGAGAGG TCATGGCTAT TGGTCGTACC TTTGAGGAGA GTTTCCAGAA AGCTTTACGG
    ATGTGCCACC CATCTATAGA AGGTTTCACT CCCCGTCTCC CAATGAACAA AGAATGGCCA
    TCTAATTTAG ATCTTAGAAA AGAGTTGTCT GAACCAAGCA GCACGCGTAT CTATGCCATT
    GCCAAGGCCA TTGATGACAA CATGTCCCTT GATGAGATTG AGAAGCTCAC ATACATTGAC
    AAGTGGTTTT TGTATAAGAT GCGTGATATT TTAAACATGG AAAAGACACT GAAAGGCCTC
    AACAGTGAGT CCATGACAGA AGAAACCCTG AAAAGGGCAA AGGAGATTGG GTTCTCAGAT
    AAGCAGATTT CAAAATGCCT TGGGCTCACT GAGGCCCAGA CAAGGGAGCT GAGGTTAAAG
    AAAAACATCC ACCCTTGGGT TAAACAGATT GATACACTGG CTGCAGAATA CCCATCAGTA
    ACAAACTATC TCTATGTTAC CTACAATGGT CAGGAGCATG ATGTCAATTT TGATGACCAT
    GGAATGATGG TGCTAGGCTG TGGTCCATAT CACATTGGCA GCAGTGTGGA ATTTGATTGG
    TGTGCTGTCT CTAGTATCCG CACACTGCGT CAACTTGGCA AGAAGACGGT GGTGGTGAAT
    TGCAATCCTG AGACTGTGAG CACAGACTTT GATGAGTGTG ACAAACTGTA CTTTGAAGAG
    TTGTCCTTGG AGAGAATCCT AGACATCTAC CATCAGGAGG CATGTGGTGG CTGCATCATA
    TCAGTTGGAG GCCAGATTCC AAACAACCTG GCAGTTCCTC TATACAAGAA TGGTGTCAAG
    ATCATGGGCA CAAGCCCCCT GCAGATCGAC AGGGCTGAGG ATCGCTCCAT CTTCTCAGCT
    GTCTTGGATG AGCTGAAGGT GGCTCAGGCA CCTTGGAAAG CTGTTAATAC TTTGAATGAA
    GCACTGGAAT TTGCAAAGTC TGTGGACTAC CCCTGCTTGT TGAGGCCTTC CTATGTTTTG
    AGTGGGTCTG CTATGAATGT GGTATTCTCT GAGGATGAGA TGAAAAAATT CCTAGAAGAG
    GCGACTAGAG TTTCTCAGGA GCACCCAGTG GTGCTGACAA AATTTGTTGA AGGGGCCCGA
    GAAGTAGAAA TGGACGCTGT TGGCAAAGAT GGAAGGGTTA TCTCTCATGC CATCTCTGAA
    CATGTTGAAG ATGCAGGTGT CCACTCGGGA GATGCCACTC TGATGCTGCC CACACAAACC
    ATCAGCCAAG GGGCCATTGA AAAGGTGAAG GATGCTACCC GGAAGATTGC AAAGGCTTTT
    GCCATCTCTG GTCCATTCAA CGTCCAATTT CTTGTCAAAG GAAATGATGT CTTGGTGATT
    GAGTGTAACT TGAGAGCTTC TCGATCCTTC CCCTTTGTTT CCAAGACTCT TGGGGTTGAC
    TTCATTGATG TGGCCACCAA GGTGATGATT GGAGAGAATG TTGATGAGAA ACATCTTCCA
    ACATTGGACC ATCCCATAAT TCCTGCTGAC TATGTTGCAA TTAAGGCTCC CATGTTTTCC
    TGGCCCCGGT TGAGGGATGC TGACCCCATT CTGAGATGTG AGATGGCTTC CACTGGAGAG
    GTGGCTTGCT TTGGTGAAGG TATTCATACA GCCTTCCTAA AGGCAATGCT TTCCACAGGA
    TTTAAGATAC CCCAGAAAGG CATCCTGATA GGCATCCAGC AATCATTCCG GCCAAGATTC
    CTTGGTGTGG CTGAACAATT ACACAATGAA GGTTTCAAGC TGTTTGCCAC GGAAGCCACA
    TCAGACTGGC TCAACGCCAA CAATGTCCCT GCCACCCCAG TGGCATGGCC GTCTCAAGAA
    GGACAGAATC CCAGCCTCTC TTCCATCAGA AAATTGATTA GAGATGGCAG CATTGACCTA
    GTGATTAACC TTCCCAACAA CAACACTAAA TTTGTCCATG ATAATTATGT GATTCGGAGG
    ACAGCTGTTG ATAGTGGAAT CCCTCTCCTC ACTAATTTTC AGGTGACCAA ACTTTTTGCT
    GAAGCTGTGC AGAAATCTCG CAAGGTGGAC TCCAAGAGTC TTTTCCACTA CAGGCAGTAC
    AGTGCTGGAA AAGCAGCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001116105.1
    CDS83..4603
    Translation

    Target ORF information:

    RefSeq Version NM_001122633.2
    Organism Homo sapiens(human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001122633.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCACAAA TCATCAAAAT GACGAGGATT TTGACAGCTT TCAAAGTGGT GAGGACACTG 
    AAGACTGGTT TTGGCTTTAC CAATGTGACT GCACACCAAA AATGGAAATT TTCAAGACCT
    GGCATCAGGC TCCTTTCTGT CAAGGCACAG ACAGCACACA TTGTCCTGGA AGATGGAACT
    AAGATGAAAG GTTACTCCTT TGGCCATCCA TCCTCTGTTG CTGGTGAAGT GGTTTTTAAT
    ACTGGCCTGG GAGGGTACCC AGAAGCTATT ACTGACCCTG CCTACAAAGG ACAGATTCTC
    ACAATGGCCA ACCCTATTAT TGGGAATGGT GGAGCTCCTG ATACTACTGC TCTGGATGAA
    CTGGGACTTA GCAAATATTT GGAGTCTAAT GGAATCAAGG TTTCAGGTTT GCTGGTGCTG
    GATTATAGTA AAGACTACAA CCACTGGCTG GCTACCAAGA GTTTAGGGCA ATGGCTACAG
    GAAGAAAAGG TTCCTGCAAT TTATGGAGTG GACACAAGAA TGCTGACTAA AATAATTCGG
    GATAAGGGTA CCATGCTTGG GAAGATTGAA TTTGAAGGTC AGCCTGTGGA TTTTGTGGAT
    CCAAATAAAC AGAATTTGAT TGCTGAGGTT TCAACCAAGG ATGTCAAAGT GTACGGCAAA
    GGAAACCCCA CAAAAGTGGT AGCTGTAGAC TGTGGGATTA AAAACAATGT AATCCGCCTG
    CTAGTAAAGC GAGGAGCTGA AGTGCACTTA GTTCCCTGGA ACCATGATTT CACCAAGATG
    GAGTATGATG GGATTTTGAT CGCGGGAGGA CCGGGGAACC CAGCTCTTGC AGAACCACTA
    ATTCAGAATG TCAGAAAGAT TTTGGAGAGT GATCGCAAGG AGCCATTGTT TGGAATCAGT
    ACAGGAAACT TAATAACAGG ATTGGCTGCT GGTGCCAAAA CCTACAAGAT GTCCATGGCC
    AACAGAGGGC AGAATCAGCC TGTTTTGAAT ATCACAAACA AACAGGCTTT CATTACTGCT
    CAGAATCATG GCTATGCCTT GGACAACACC CTCCCTGCTG GCTGGAAACC ACTTTTTGTG
    AATGTCAACG ATCAAACAAA TGAGGGGATT ATGCATGAGA GCAAACCCTT CTTCGCTGTG
    CAGTTCCACC CAGAGGTCAC CCCGGGGCCA ATAGACACTG AGTACCTGTT TGATTCCTTT
    TTCTCACTGA TAAAGAAAGG AAAAGCTACC ACCATTACAT CAGTCTTACC GAAGCCAGCA
    CTAGTTGCAT CTCGGGTTGA GGTTTCCAAA GTCCTTATTC TAGGATCAGG AGGTCTGTCC
    ATTGGTCAGG CTGGAGAATT TGATTACTCA GGATCTCAAG CTGTAAAAGC CATGAAGGAA
    GAAAATGTCA AAACTGTTCT GATGAACCCA AACATTGCAT CAGTCCAGAC CAATGAGGTG
    GGCTTAAAGC AAGCGGATAC TGTCTACTTT CTTCCCATCA CCCCTCAGTT TGTCACAGAG
    GTCATCAAGG CAGAACAGCC AGATGGGTTA ATTCTGGGCA TGGGTGGCCA GACAGCTCTG
    AACTGTGGAG TGGAACTATT CAAGAGAGGT GTGCTCAAGG AATATGGTGT GAAAGTCCTG
    GGAACTTCAG TTGAGTCCAT TATGGCTACG GAAGACAGGC AGCTGTTTTC AGATAAACTA
    AATGAGATCA ATGAAAAGAT TGCTCCAAGT TTTGCAGTGG AATCGATTGA GGATGCACTG
    AAGGCAGCAG ACACCATTGG CTACCCAGTG ATGATCCGTT CCGCCTATGC ACTGGGTGGG
    TTAGGCTCAG GCATCTGTCC CAACAGAGAG ACTTTGATGG ACCTCAGCAC AAAGGCCTTT
    GCTATGACCA ACCAAATTCT GGTGGAGAAG TCAGTGACAG GTTGGAAAGA AATAGAATAT
    GAAGTGGTTC GAGATGCTGA TGACAATTGT GTCACTGTCT GTAACATGGA AAATGTTGAT
    GCCATGGGTG TTCACACAGG TGACTCAGTT GTTGTGGCTC CTGCCCAGAC ACTCTCCAAT
    GCCGAGTTTC AGATGTTGAG ACGTACTTCA ATCAATGTTG TTCGCCACTT GGGCATTGTG
    GGTGAATGCA ACATTCAGTT TGCCCTTCAT CCTACCTCAA TGGAATACTG CATCATTGAA
    GTGAATGCCA GACTGTCCCG AAGCTCTGCT CTGGCCTCAA AAGCCACTGG CTACCCATTG
    GCATTCATTG CTGCAAAGAT TGCCCTAGGA ATCCCACTTC CAGAAATTAA GAACGTCGTA
    TCCGGGAAGA CATCAGCCTG TTTTGAACCT AGCCTGGATT ACATGGTCAC CAAGATTCCC
    CGCTGGGATC TTGACCGTTT TCATGGAACA TCTAGCCGAA TTGGTAGCTC TATGAAAAGT
    GTAGGAGAGG TCATGGCTAT TGGTCGTACC TTTGAGGAGA GTTTCCAGAA AGCTTTACGG
    ATGTGCCACC CATCTATAGA AGGTTTCACT CCCCGTCTCC CAATGAACAA AGAATGGCCA
    TCTAATTTAG ATCTTAGAAA AGAGTTGTCT GAACCAAGCA GCACGCGTAT CTATGCCATT
    GCCAAGGCCA TTGATGACAA CATGTCCCTT GATGAGATTG AGAAGCTCAC ATACATTGAC
    AAGTGGTTTT TGTATAAGAT GCGTGATATT TTAAACATGG AAAAGACACT GAAAGGCCTC
    AACAGTGAGT CCATGACAGA AGAAACCCTG AAAAGGGCAA AGGAGATTGG GTTCTCAGAT
    AAGCAGATTT CAAAATGCCT TGGGCTCACT GAGGCCCAGA CAAGGGAGCT GAGGTTAAAG
    AAAAACATCC ACCCTTGGGT TAAACAGATT GATACACTGG CTGCAGAATA CCCATCAGTA
    ACAAACTATC TCTATGTTAC CTACAATGGT CAGGAGCATG ATGTCAATTT TGATGACCAT
    GGAATGATGG TGCTAGGCTG TGGTCCATAT CACATTGGCA GCAGTGTGGA ATTTGATTGG
    TGTGCTGTCT CTAGTATCCG CACACTGCGT CAACTTGGCA AGAAGACGGT GGTGGTGAAT
    TGCAATCCTG AGACTGTGAG CACAGACTTT GATGAGTGTG ACAAACTGTA CTTTGAAGAG
    TTGTCCTTGG AGAGAATCCT AGACATCTAC CATCAGGAGG CATGTGGTGG CTGCATCATA
    TCAGTTGGAG GCCAGATTCC AAACAACCTG GCAGTTCCTC TATACAAGAA TGGTGTCAAG
    ATCATGGGCA CAAGCCCCCT GCAGATCGAC AGGGCTGAGG ATCGCTCCAT CTTCTCAGCT
    GTCTTGGATG AGCTGAAGGT GGCTCAGGCA CCTTGGAAAG CTGTTAATAC TTTGAATGAA
    GCACTGGAAT TTGCAAAGTC TGTGGACTAC CCCTGCTTGT TGAGGCCTTC CTATGTTTTG
    AGTGGGTCTG CTATGAATGT GGTATTCTCT GAGGATGAGA TGAAAAAATT CCTAGAAGAG
    GCGACTAGAG TTTCTCAGGA GCACCCAGTG GTGCTGACAA AATTTGTTGA AGGGGCCCGA
    GAAGTAGAAA TGGACGCTGT TGGCAAAGAT GGAAGGGTTA TCTCTCATGC CATCTCTGAA
    CATGTTGAAG ATGCAGGTGT CCACTCGGGA GATGCCACTC TGATGCTGCC CACACAAACC
    ATCAGCCAAG GGGCCATTGA AAAGGTGAAG GATGCTACCC GGAAGATTGC AAAGGCTTTT
    GCCATCTCTG GTCCATTCAA CGTCCAATTT CTTGTCAAAG GAAATGATGT CTTGGTGATT
    GAGTGTAACT TGAGAGCTTC TCGATCCTTC CCCTTTGTTT CCAAGACTCT TGGGGTTGAC
    TTCATTGATG TGGCCACCAA GGTGATGATT GGAGAGAATG TTGATGAGAA ACATCTTCCA
    ACATTGGACC ATCCCATAAT TCCTGCTGAC TATGTTGCAA TTAAGGCTCC CATGTTTTCC
    TGGCCCCGGT TGAGGGATGC TGACCCCATT CTGAGATGTG AGATGGCTTC CACTGGAGAG
    GTGGCTTGCT TTGGTGAAGG TATTCATACA GCCTTCCTAA AGGCAATGCT TTCCACAGGA
    TTTAAGATAC CCCAGAAAGG CATCCTGATA GGCATCCAGC AATCATTCCG GCCAAGATTC
    CTTGGTGTGG CTGAACAATT ACACAATGAA GGTTTCAAGC TGTTTGCCAC GGAAGCCACA
    TCAGACTGGC TCAACGCCAA CAATGTCCCT GCCACCCCAG TGGCATGGCC GTCTCAAGAA
    GGACAGAATC CCAGCCTCTC TTCCATCAGA AAATTGATTA GAGATGGCAG CATTGACCTA
    GTGATTAACC TTCCCAACAA CAACACTAAA TTTGTCCATG ATAATTATGT GATTCGGAGG
    ACAGCTGTTG ATAGTGGAAT CCCTCTCCTC ACTAATTTTC AGGTGACCAA ACTTTTTGCT
    GAAGCTGTGC AGAAATCTCG CAAGGTGGAC TCCAAGAGTC TTTTCCACTA CAGGCAGTAC
    AGTGCTGGAA AAGCAGCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24805
    Clone ID Related Accession (Same CDS sequence) XM_011510643.1 , XM_011510643.2 , XM_011510644.1 , NM_001369257.1 , NM_001875.5 , XM_011510642.2 , XM_011510644.2 , XM_011510642.1 , XM_011510641.1 , NM_001875.4 , XM_011510641.2 , NM_001122633.3
    Accession Version NM_001369257.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-16
    Organism Homo sapiens(human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform b precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007970.3 and AC008172.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.248665.1, SRR1660803.140911.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001356186.1
    CDS218..4720
    Translation

    Target ORF information:

    RefSeq Version NM_001369257.1
    Organism Homo sapiens(human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001369257.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24805
    Clone ID Related Accession (Same CDS sequence) XM_011510643.1 , XM_011510643.2 , XM_011510644.1 , NM_001369257.1 , NM_001875.5 , XM_011510642.2 , XM_011510644.2 , XM_011510642.1 , XM_011510641.1 , NM_001875.4 , XM_011510641.2 , NM_001122633.3
    Accession Version NM_001875.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform b precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BJ995129.1, BC140943.1 and BC058010.1. On May 17, 2019 this sequence version replaced NM_001875.4. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF154830.1, SRR1660805.216985.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000233072.10/ ENSP00000233072.5 RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001866.2
    CDS136..4638
    Translation

    Target ORF information:

    RefSeq Version NM_001875.5
    Organism Homo sapiens(human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001875.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24805
    Clone ID Related Accession (Same CDS sequence) XM_011510643.1 , XM_011510643.2 , XM_011510644.1 , NM_001369257.1 , NM_001875.5 , XM_011510642.2 , XM_011510644.2 , XM_011510642.1 , XM_011510641.1 , NM_001875.4 , XM_011510641.2 , NM_001122633.3
    Accession Version NM_001875.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform b precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BJ995129.1, BC140943.1 and BC058010.1. This sequence is a reference standard in the RefSeqGene project. On Apr 6, 2011 this sequence version replaced NM_001875.3. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF154830.1, SRR1660805.216985.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001866.2
    CDS136..4638
    Translation

    Target ORF information:

    RefSeq Version NM_001875.4
    Organism Homo sapiens(human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001875.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24805
    Clone ID Related Accession (Same CDS sequence) XM_011510643.1 , XM_011510643.2 , XM_011510644.1 , NM_001369257.1 , NM_001875.5 , XM_011510642.2 , XM_011510644.2 , XM_011510642.1 , XM_011510641.1 , NM_001875.4 , XM_011510641.2 , NM_001122633.3
    Accession Version NM_001122633.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-10
    Organism Homo sapiens(human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007970.3, AB208800.1, Y15793.1 and N58033.1. On May 29, 2019 this sequence version replaced NM_001122633.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB208800.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001116105.2
    CDS98..4600
    Translation

    Target ORF information:

    RefSeq Version NM_001122633.3
    Organism Homo sapiens(human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001122633.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25055
    Clone ID Related Accession (Same CDS sequence) NM_001122634.3
    Accession Version NM_001122634.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK316425.1, AY317138.1, AB208800.1, AC008172.2 and AV645723.1. On Jan 30, 2015 this sequence version replaced NM_001122634.2. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a and may not be localized to the mitochondrion. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY317138.1, AK302778.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAGGAAG AAAATGTCAA AACTGTTCTG ATGAACCCAA ACATTGCATC AGTCCAGACC 
    AATGAGGTGG GCTTAAAGCA AGCGGATACT GTCTACTTTC TTCCCATCAC CCCTCAGTTT
    GTCACAGAGG TCATCAAGGC AGAACAGCCA GATGGGTTAA TTCTGGGCAT GGGTGGCCAG
    ACAGCTCTGA ACTGTGGAGT GGAACTATTC AAGAGAGGTG TGCTCAAGGA ATATGGTGTG
    AAAGTCCTGG GAACTTCAGT TGAGTCCATT ATGGCTACGG AAGACAGGCA GCTGTTTTCA
    GATAAACTAA ATGAGATCAA TGAAAAGATT GCTCCAAGTT TTGCAGTGGA ATCGATTGAG
    GATGCACTGA AGGCAGCAGA CACCATTGGC TACCCAGTGA TGATCCGTTC CGCCTATGCA
    CTGGGTGGGT TAGGCTCAGG CATCTGTCCC AACAGAGAGA CTTTGATGGA CCTCAGCACA
    AAGGCCTTTG CTATGACCAA CCAAATTCTG GTGGAGAAGT CAGTGACAGG TTGGAAAGAA
    ATAGAATATG AAGTGGTTCG AGATGCTGAT GACAATTGTG TCACTGTCTG TAACATGGAA
    AATGTTGATG CCATGGGTGT TCACACAGGT GACTCAGTTG TTGTGGCTCC TGCCCAGACA
    CTCTCCAATG CCGAGTTTCA GATGTTGAGA CGTACTTCAA TCAATGTTGT TCGCCACTTG
    GGCATTGTGG GTGAATGCAA CATTCAGTTT GCCCTTCATC CTACCTCAAT GGAATACTGC
    ATCATTGAAG TGAATGCCAG ACTGTCCCGA AGCTCTGCTC TGGCCTCAAA AGCCACTGGC
    TACCCATTGG CATTCATTGC TGCAAAGATT GCCCTAGGAA TCCCACTTCC AGAAATTAAG
    AACGTCGTAT CCGGGAAGAC ATCAGCCTGT TTTGAACCTA GCCTGGATTA CATGGTCACC
    AAGATTCCCC GCTGGGATCT TGACCGTTTT CATGGAACAT CTAGCCGAAT TGGTAGCTCT
    ATGAAAAGTG TAGGAGAGGT CATGGCTATT GGTCGTACCT TTGAGGAGAG TTTCCAGAAA
    GCTTTACGGA TGTGCCACCC ATCTATAGAA GGTTTCACTC CCCGTCTCCC AATGAACAAA
    GAATGGCCAT CTAATTTAGA TCTTAGAAAA GAGTTGTCTG AACCAAGCAG CACGCGTATC
    TATGCCATTG CCAAGGCCAT TGATGACAAC ATGTCCCTTG ATGAGATTGA GAAGCTCACA
    TACATTGACA AGTGGTTTTT GTATAAGATG CGTGATATTT TAAACATGGA AAAGACACTG
    AAAGGCCTCA ACAGTGAGTC CATGACAGAA GAAACCCTGA AAAGGGCAAA GGAGATTGGG
    TTCTCAGATA AGCAGATTTC AAAATGCCTT GGGCTCACTG AGGCCCAGAC AAGGGAGCTG
    AGGTTAAAGA AAAACATCCA CCCTTGGGTT AAACAGATTG ATACACTGGC TGCAGAATAC
    CCATCAGTAA CAAACTATCT CTATGTTACC TACAATGGTC AGGAGCATGA TGTCAATTTT
    GATGACCATG GAATGATGGT GCTAGGCTGT GGTCCATATC ACATTGGCAG CAGTGTGGAA
    TTTGATTGGT GTGCTGTCTC TAGTATCCGC ACACTGCGTC AACTTGGCAA GAAGACGGTG
    GTGGTGAATT GCAATCCTGA GACTGTGAGC ACAGACTTTG ATGAGTGTGA CAAACTGTAC
    TTTGAAGAGT TGTCCTTGGA GAGAATCCTA GACATCTACC ATCAGGAGGC ATGTGGTGGC
    TGCATCATAT CAGTTGGAGG CCAGATTCCA AACAACCTGG CAGTTCCTCT ATACAAGAAT
    GGTGTCAAGA TCATGGGCAC AAGCCCCCTG CAGATCGACA GGGCTGAGGA TCGCTCCATC
    TTCTCAGCTG TCTTGGATGA GCTGAAGGTG GCTCAGGCAC CTTGGAAAGC TGTTAATACT
    TTGAATGAAG CACTGGAATT TGCAAAGTCT GTGGACTACC CCTGCTTGTT GAGGCCTTCC
    TATGTTTTGA GTGGGTCTGC TATGAATGTG GTATTCTCTG AGGATGAGAT GAAAAAATTC
    CTAGAAGAGG CGACTAGAGT TTCTCAGGAG CACCCAGTGG TGCTGACAAA ATTTGTTGAA
    GGGGCCCGAG AAGTAGAAAT GGACGCTGTT GGCAAAGATG GAAGGGTTAT CTCTCATGCC
    ATCTCTGAAC ATGTTGAAGA TGCAGGTGTC CACTCGGGAG ATGCCACTCT GATGCTGCCC
    ACACAAACCA TCAGCCAAGG GGCCATTGAA AAGGTGAAGG ATGCTACCCG GAAGATTGCA
    AAGGCTTTTG CCATCTCTGG TCCATTCAAC GTCCAATTTC TTGTCAAAGG AAATGATGTC
    TTGGTGATTG AGTGTAACTT GAGAGCTTCT CGATCCTTCC CCTTTGTTTC CAAGACTCTT
    GGGGTTGACT TCATTGATGT GGCCACCAAG GTGATGATTG GAGAGAATGT TGATGAGAAA
    CATCTTCCAA CATTGGACCA TCCCATAATT CCTGCTGACT ATGTTGCAAT TAAGGCTCCC
    ATGTTTTCCT GGCCCCGGTT GAGGGATGCT GACCCCATTC TGAGATGTGA GATGGCTTCC
    ACTGGAGAGG TGGCTTGCTT TGGTGAAGGT ATTCATACAG CCTTCCTAAA GGCAATGCTT
    TCCACAGGAT TTAAGATACC CCAGAAAGGC ATCCTGATAG GCATCCAGCA ATCATTCCGG
    CCAAGATTCC TTGGTGTGGC TGAACAATTA CACAATGAAG GTTTCAAGCT GTTTGCCACG
    GAAGCCACAT CAGACTGGCT CAACGCCAAC AATGTCCCTG CCACCCCAGT GGCATGGCCG
    TCTCAAGAAG GACAGAATCC CAGCCTCTCT TCCATCAGAA AATTGATTAG AGATGGCAGC
    ATTGACCTAG TGATTAACCT TCCCAACAAC AACACTAAAT TTGTCCATGA TAATTATGTG
    ATTCGGAGGA CAGCTGTTGA TAGTGGAATC CCTCTCCTCA CTAATTTTCA GGTGACCAAA
    CTTTTTGCTG AAGCTGTGCA GAAATCTCGC AAGGTGGACT CCAAGAGTCT TTTCCACTAC
    AGGCAGTACA GTGCTGGAAA AGCAGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001116106.1
    CDS510..3659
    Translation

    Target ORF information:

    RefSeq Version NM_001122634.3
    Organism Homo sapiens(human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001122634.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAGGAAG AAAATGTCAA AACTGTTCTG ATGAACCCAA ACATTGCATC AGTCCAGACC 
    AATGAGGTGG GCTTAAAGCA AGCGGATACT GTCTACTTTC TTCCCATCAC CCCTCAGTTT
    GTCACAGAGG TCATCAAGGC AGAACAGCCA GATGGGTTAA TTCTGGGCAT GGGTGGCCAG
    ACAGCTCTGA ACTGTGGAGT GGAACTATTC AAGAGAGGTG TGCTCAAGGA ATATGGTGTG
    AAAGTCCTGG GAACTTCAGT TGAGTCCATT ATGGCTACGG AAGACAGGCA GCTGTTTTCA
    GATAAACTAA ATGAGATCAA TGAAAAGATT GCTCCAAGTT TTGCAGTGGA ATCGATTGAG
    GATGCACTGA AGGCAGCAGA CACCATTGGC TACCCAGTGA TGATCCGTTC CGCCTATGCA
    CTGGGTGGGT TAGGCTCAGG CATCTGTCCC AACAGAGAGA CTTTGATGGA CCTCAGCACA
    AAGGCCTTTG CTATGACCAA CCAAATTCTG GTGGAGAAGT CAGTGACAGG TTGGAAAGAA
    ATAGAATATG AAGTGGTTCG AGATGCTGAT GACAATTGTG TCACTGTCTG TAACATGGAA
    AATGTTGATG CCATGGGTGT TCACACAGGT GACTCAGTTG TTGTGGCTCC TGCCCAGACA
    CTCTCCAATG CCGAGTTTCA GATGTTGAGA CGTACTTCAA TCAATGTTGT TCGCCACTTG
    GGCATTGTGG GTGAATGCAA CATTCAGTTT GCCCTTCATC CTACCTCAAT GGAATACTGC
    ATCATTGAAG TGAATGCCAG ACTGTCCCGA AGCTCTGCTC TGGCCTCAAA AGCCACTGGC
    TACCCATTGG CATTCATTGC TGCAAAGATT GCCCTAGGAA TCCCACTTCC AGAAATTAAG
    AACGTCGTAT CCGGGAAGAC ATCAGCCTGT TTTGAACCTA GCCTGGATTA CATGGTCACC
    AAGATTCCCC GCTGGGATCT TGACCGTTTT CATGGAACAT CTAGCCGAAT TGGTAGCTCT
    ATGAAAAGTG TAGGAGAGGT CATGGCTATT GGTCGTACCT TTGAGGAGAG TTTCCAGAAA
    GCTTTACGGA TGTGCCACCC ATCTATAGAA GGTTTCACTC CCCGTCTCCC AATGAACAAA
    GAATGGCCAT CTAATTTAGA TCTTAGAAAA GAGTTGTCTG AACCAAGCAG CACGCGTATC
    TATGCCATTG CCAAGGCCAT TGATGACAAC ATGTCCCTTG ATGAGATTGA GAAGCTCACA
    TACATTGACA AGTGGTTTTT GTATAAGATG CGTGATATTT TAAACATGGA AAAGACACTG
    AAAGGCCTCA ACAGTGAGTC CATGACAGAA GAAACCCTGA AAAGGGCAAA GGAGATTGGG
    TTCTCAGATA AGCAGATTTC AAAATGCCTT GGGCTCACTG AGGCCCAGAC AAGGGAGCTG
    AGGTTAAAGA AAAACATCCA CCCTTGGGTT AAACAGATTG ATACACTGGC TGCAGAATAC
    CCATCAGTAA CAAACTATCT CTATGTTACC TACAATGGTC AGGAGCATGA TGTCAATTTT
    GATGACCATG GAATGATGGT GCTAGGCTGT GGTCCATATC ACATTGGCAG CAGTGTGGAA
    TTTGATTGGT GTGCTGTCTC TAGTATCCGC ACACTGCGTC AACTTGGCAA GAAGACGGTG
    GTGGTGAATT GCAATCCTGA GACTGTGAGC ACAGACTTTG ATGAGTGTGA CAAACTGTAC
    TTTGAAGAGT TGTCCTTGGA GAGAATCCTA GACATCTACC ATCAGGAGGC ATGTGGTGGC
    TGCATCATAT CAGTTGGAGG CCAGATTCCA AACAACCTGG CAGTTCCTCT ATACAAGAAT
    GGTGTCAAGA TCATGGGCAC AAGCCCCCTG CAGATCGACA GGGCTGAGGA TCGCTCCATC
    TTCTCAGCTG TCTTGGATGA GCTGAAGGTG GCTCAGGCAC CTTGGAAAGC TGTTAATACT
    TTGAATGAAG CACTGGAATT TGCAAAGTCT GTGGACTACC CCTGCTTGTT GAGGCCTTCC
    TATGTTTTGA GTGGGTCTGC TATGAATGTG GTATTCTCTG AGGATGAGAT GAAAAAATTC
    CTAGAAGAGG CGACTAGAGT TTCTCAGGAG CACCCAGTGG TGCTGACAAA ATTTGTTGAA
    GGGGCCCGAG AAGTAGAAAT GGACGCTGTT GGCAAAGATG GAAGGGTTAT CTCTCATGCC
    ATCTCTGAAC ATGTTGAAGA TGCAGGTGTC CACTCGGGAG ATGCCACTCT GATGCTGCCC
    ACACAAACCA TCAGCCAAGG GGCCATTGAA AAGGTGAAGG ATGCTACCCG GAAGATTGCA
    AAGGCTTTTG CCATCTCTGG TCCATTCAAC GTCCAATTTC TTGTCAAAGG AAATGATGTC
    TTGGTGATTG AGTGTAACTT GAGAGCTTCT CGATCCTTCC CCTTTGTTTC CAAGACTCTT
    GGGGTTGACT TCATTGATGT GGCCACCAAG GTGATGATTG GAGAGAATGT TGATGAGAAA
    CATCTTCCAA CATTGGACCA TCCCATAATT CCTGCTGACT ATGTTGCAAT TAAGGCTCCC
    ATGTTTTCCT GGCCCCGGTT GAGGGATGCT GACCCCATTC TGAGATGTGA GATGGCTTCC
    ACTGGAGAGG TGGCTTGCTT TGGTGAAGGT ATTCATACAG CCTTCCTAAA GGCAATGCTT
    TCCACAGGAT TTAAGATACC CCAGAAAGGC ATCCTGATAG GCATCCAGCA ATCATTCCGG
    CCAAGATTCC TTGGTGTGGC TGAACAATTA CACAATGAAG GTTTCAAGCT GTTTGCCACG
    GAAGCCACAT CAGACTGGCT CAACGCCAAC AATGTCCCTG CCACCCCAGT GGCATGGCCG
    TCTCAAGAAG GACAGAATCC CAGCCTCTCT TCCATCAGAA AATTGATTAG AGATGGCAGC
    ATTGACCTAG TGATTAACCT TCCCAACAAC AACACTAAAT TTGTCCATGA TAATTATGTG
    ATTCGGAGGA CAGCTGTTGA TAGTGGAATC CCTCTCCTCA CTAATTTTCA GGTGACCAAA
    CTTTTTGCTG AAGCTGTGCA GAAATCTCGC AAGGTGGACT CCAAGAGTCT TTTCCACTAC
    AGGCAGTACA GTGCTGGAAA AGCAGCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24805
    Clone ID Related Accession (Same CDS sequence) XM_011510643.1 , XM_011510643.2 , XM_011510644.1 , NM_001369257.1 , NM_001875.5 , XM_011510642.2 , XM_011510644.2 , XM_011510642.1 , XM_011510641.1 , NM_001875.4 , XM_011510641.2 , NM_001122633.3
    Accession Version XM_011510643.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508945.1
    Translation

    Target ORF information:

    RefSeq Version XM_011510643.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1, mitochondrial (CPS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24805
    Clone ID Related Accession (Same CDS sequence) XM_011510643.1 , XM_011510643.2 , XM_011510644.1 , NM_001369257.1 , NM_001875.5 , XM_011510642.2 , XM_011510644.2 , XM_011510642.1 , XM_011510641.1 , NM_001875.4 , XM_011510641.2 , NM_001122633.3
    Accession Version XM_011510643.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508945.1
    CDS216..4718
    Translation

    Target ORF information:

    RefSeq Version XM_011510643.2
    Organism Homo sapiens(human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510643.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24805
    Clone ID Related Accession (Same CDS sequence) XM_011510643.1 , XM_011510643.2 , XM_011510644.1 , NM_001369257.1 , NM_001875.5 , XM_011510642.2 , XM_011510644.2 , XM_011510642.1 , XM_011510641.1 , NM_001875.4 , XM_011510641.2 , NM_001122633.3
    Accession Version XM_011510644.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508946.1
    Translation

    Target ORF information:

    RefSeq Version XM_011510644.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1, mitochondrial (CPS1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24805
    Clone ID Related Accession (Same CDS sequence) XM_011510643.1 , XM_011510643.2 , XM_011510644.1 , NM_001369257.1 , NM_001875.5 , XM_011510642.2 , XM_011510644.2 , XM_011510642.1 , XM_011510641.1 , NM_001875.4 , XM_011510641.2 , NM_001122633.3
    Accession Version XM_011510642.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510642.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508944.1
    CDS351..4853
    Translation

    Target ORF information:

    RefSeq Version XM_011510642.2
    Organism Homo sapiens(human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510642.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24805
    Clone ID Related Accession (Same CDS sequence) XM_011510643.1 , XM_011510643.2 , XM_011510644.1 , NM_001369257.1 , NM_001875.5 , XM_011510642.2 , XM_011510644.2 , XM_011510642.1 , XM_011510641.1 , NM_001875.4 , XM_011510641.2 , NM_001122633.3
    Accession Version XM_011510644.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508946.1
    CDS257..4759
    Translation

    Target ORF information:

    RefSeq Version XM_011510644.2
    Organism Homo sapiens(human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510644.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24805
    Clone ID Related Accession (Same CDS sequence) XM_011510643.1 , XM_011510643.2 , XM_011510644.1 , NM_001369257.1 , NM_001875.5 , XM_011510642.2 , XM_011510644.2 , XM_011510642.1 , XM_011510641.1 , NM_001875.4 , XM_011510641.2 , NM_001122633.3
    Accession Version XM_011510642.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508944.1
    Translation

    Target ORF information:

    RefSeq Version XM_011510642.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1, mitochondrial (CPS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24805
    Clone ID Related Accession (Same CDS sequence) XM_011510643.1 , XM_011510643.2 , XM_011510644.1 , NM_001369257.1 , NM_001875.5 , XM_011510642.2 , XM_011510644.2 , XM_011510642.1 , XM_011510641.1 , NM_001875.4 , XM_011510641.2 , NM_001122633.3
    Accession Version XM_011510641.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508943.1
    Translation

    Target ORF information:

    RefSeq Version XM_011510641.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1, mitochondrial (CPS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510641.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24805
    Clone ID Related Accession (Same CDS sequence) XM_011510643.1 , XM_011510643.2 , XM_011510644.1 , NM_001369257.1 , NM_001875.5 , XM_011510642.2 , XM_011510644.2 , XM_011510642.1 , XM_011510641.1 , NM_001875.4 , XM_011510641.2 , NM_001122633.3
    Accession Version XM_011510641.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510641.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508943.1
    CDS479..4981
    Translation

    Target ORF information:

    RefSeq Version XM_011510641.2
    Organism Homo sapiens(human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510641.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACGAGGA TTTTGACAGC TTTCAAAGTG GTGAGGACAC TGAAGACTGG TTTTGGCTTT 
    ACCAATGTGA CTGCACACCA AAAATGGAAA TTTTCAAGAC CTGGCATCAG GCTCCTTTCT
    GTCAAGGCAC AGACAGCACA CATTGTCCTG GAAGATGGAA CTAAGATGAA AGGTTACTCC
    TTTGGCCATC CATCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCCT GGGAGGGTAC
    CCAGAAGCTA TTACTGACCC TGCCTACAAA GGACAGATTC TCACAATGGC CAACCCTATT
    ATTGGGAATG GTGGAGCTCC TGATACTACT GCTCTGGATG AACTGGGACT TAGCAAATAT
    TTGGAGTCTA ATGGAATCAA GGTTTCAGGT TTGCTGGTGC TGGATTATAG TAAAGACTAC
    AACCACTGGC TGGCTACCAA GAGTTTAGGG CAATGGCTAC AGGAAGAAAA GGTTCCTGCA
    ATTTATGGAG TGGACACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT
    GGGAAGATTG AATTTGAAGG TCAGCCTGTG GATTTTGTGG ATCCAAATAA ACAGAATTTG
    ATTGCTGAGG TTTCAACCAA GGATGTCAAA GTGTACGGCA AAGGAAACCC CACAAAAGTG
    GTAGCTGTAG ACTGTGGGAT TAAAAACAAT GTAATCCGCC TGCTAGTAAA GCGAGGAGCT
    GAAGTGCACT TAGTTCCCTG GAACCATGAT TTCACCAAGA TGGAGTATGA TGGGATTTTG
    ATCGCGGGAG GACCGGGGAA CCCAGCTCTT GCAGAACCAC TAATTCAGAA TGTCAGAAAG
    ATTTTGGAGA GTGATCGCAA GGAGCCATTG TTTGGAATCA GTACAGGAAA CTTAATAACA
    GGATTGGCTG CTGGTGCCAA AACCTACAAG ATGTCCATGG CCAACAGAGG GCAGAATCAG
    CCTGTTTTGA ATATCACAAA CAAACAGGCT TTCATTACTG CTCAGAATCA TGGCTATGCC
    TTGGACAACA CCCTCCCTGC TGGCTGGAAA CCACTTTTTG TGAATGTCAA CGATCAAACA
    AATGAGGGGA TTATGCATGA GAGCAAACCC TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC
    ACCCCGGGGC CAATAGACAC TGAGTACCTG TTTGATTCCT TTTTCTCACT GATAAAGAAA
    GGAAAAGCTA CCACCATTAC ATCAGTCTTA CCGAAGCCAG CACTAGTTGC ATCTCGGGTT
    GAGGTTTCCA AAGTCCTTAT TCTAGGATCA GGAGGTCTGT CCATTGGTCA GGCTGGAGAA
    TTTGATTACT CAGGATCTCA AGCTGTAAAA GCCATGAAGG AAGAAAATGT CAAAACTGTT
    CTGATGAACC CAAACATTGC ATCAGTCCAG ACCAATGAGG TGGGCTTAAA GCAAGCGGAT
    ACTGTCTACT TTCTTCCCAT CACCCCTCAG TTTGTCACAG AGGTCATCAA GGCAGAACAG
    CCAGATGGGT TAATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA
    TTCAAGAGAG GTGTGCTCAA GGAATATGGT GTGAAAGTCC TGGGAACTTC AGTTGAGTCC
    ATTATGGCTA CGGAAGACAG GCAGCTGTTT TCAGATAAAC TAAATGAGAT CAATGAAAAG
    ATTGCTCCAA GTTTTGCAGT GGAATCGATT GAGGATGCAC TGAAGGCAGC AGACACCATT
    GGCTACCCAG TGATGATCCG TTCCGCCTAT GCACTGGGTG GGTTAGGCTC AGGCATCTGT
    CCCAACAGAG AGACTTTGAT GGACCTCAGC ACAAAGGCCT TTGCTATGAC CAACCAAATT
    CTGGTGGAGA AGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTGGT TCGAGATGCT
    GATGACAATT GTGTCACTGT CTGTAACATG GAAAATGTTG ATGCCATGGG TGTTCACACA
    GGTGACTCAG TTGTTGTGGC TCCTGCCCAG ACACTCTCCA ATGCCGAGTT TCAGATGTTG
    AGACGTACTT CAATCAATGT TGTTCGCCAC TTGGGCATTG TGGGTGAATG CAACATTCAG
    TTTGCCCTTC ATCCTACCTC AATGGAATAC TGCATCATTG AAGTGAATGC CAGACTGTCC
    CGAAGCTCTG CTCTGGCCTC AAAAGCCACT GGCTACCCAT TGGCATTCAT TGCTGCAAAG
    ATTGCCCTAG GAATCCCACT TCCAGAAATT AAGAACGTCG TATCCGGGAA GACATCAGCC
    TGTTTTGAAC CTAGCCTGGA TTACATGGTC ACCAAGATTC CCCGCTGGGA TCTTGACCGT
    TTTCATGGAA CATCTAGCCG AATTGGTAGC TCTATGAAAA GTGTAGGAGA GGTCATGGCT
    ATTGGTCGTA CCTTTGAGGA GAGTTTCCAG AAAGCTTTAC GGATGTGCCA CCCATCTATA
    GAAGGTTTCA CTCCCCGTCT CCCAATGAAC AAAGAATGGC CATCTAATTT AGATCTTAGA
    AAAGAGTTGT CTGAACCAAG CAGCACGCGT ATCTATGCCA TTGCCAAGGC CATTGATGAC
    AACATGTCCC TTGATGAGAT TGAGAAGCTC ACATACATTG ACAAGTGGTT TTTGTATAAG
    ATGCGTGATA TTTTAAACAT GGAAAAGACA CTGAAAGGCC TCAACAGTGA GTCCATGACA
    GAAGAAACCC TGAAAAGGGC AAAGGAGATT GGGTTCTCAG ATAAGCAGAT TTCAAAATGC
    CTTGGGCTCA CTGAGGCCCA GACAAGGGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG
    GTTAAACAGA TTGATACACT GGCTGCAGAA TACCCATCAG TAACAAACTA TCTCTATGTT
    ACCTACAATG GTCAGGAGCA TGATGTCAAT TTTGATGACC ATGGAATGAT GGTGCTAGGC
    TGTGGTCCAT ATCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCTAGTATC
    CGCACACTGC GTCAACTTGG CAAGAAGACG GTGGTGGTGA ATTGCAATCC TGAGACTGTG
    AGCACAGACT TTGATGAGTG TGACAAACTG TACTTTGAAG AGTTGTCCTT GGAGAGAATC
    CTAGACATCT ACCATCAGGA GGCATGTGGT GGCTGCATCA TATCAGTTGG AGGCCAGATT
    CCAAACAACC TGGCAGTTCC TCTATACAAG AATGGTGTCA AGATCATGGG CACAAGCCCC
    CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTGGA TGAGCTGAAG
    GTGGCTCAGG CACCTTGGAA AGCTGTTAAT ACTTTGAATG AAGCACTGGA ATTTGCAAAG
    TCTGTGGACT ACCCCTGCTT GTTGAGGCCT TCCTATGTTT TGAGTGGGTC TGCTATGAAT
    GTGGTATTCT CTGAGGATGA GATGAAAAAA TTCCTAGAAG AGGCGACTAG AGTTTCTCAG
    GAGCACCCAG TGGTGCTGAC AAAATTTGTT GAAGGGGCCC GAGAAGTAGA AATGGACGCT
    GTTGGCAAAG ATGGAAGGGT TATCTCTCAT GCCATCTCTG AACATGTTGA AGATGCAGGT
    GTCCACTCGG GAGATGCCAC TCTGATGCTG CCCACACAAA CCATCAGCCA AGGGGCCATT
    GAAAAGGTGA AGGATGCTAC CCGGAAGATT GCAAAGGCTT TTGCCATCTC TGGTCCATTC
    AACGTCCAAT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGTAA CTTGAGAGCT
    TCTCGATCCT TCCCCTTTGT TTCCAAGACT CTTGGGGTTG ACTTCATTGA TGTGGCCACC
    AAGGTGATGA TTGGAGAGAA TGTTGATGAG AAACATCTTC CAACATTGGA CCATCCCATA
    ATTCCTGCTG ACTATGTTGC AATTAAGGCT CCCATGTTTT CCTGGCCCCG GTTGAGGGAT
    GCTGACCCCA TTCTGAGATG TGAGATGGCT TCCACTGGAG AGGTGGCTTG CTTTGGTGAA
    GGTATTCATA CAGCCTTCCT AAAGGCAATG CTTTCCACAG GATTTAAGAT ACCCCAGAAA
    GGCATCCTGA TAGGCATCCA GCAATCATTC CGGCCAAGAT TCCTTGGTGT GGCTGAACAA
    TTACACAATG AAGGTTTCAA GCTGTTTGCC ACGGAAGCCA CATCAGACTG GCTCAACGCC
    AACAATGTCC CTGCCACCCC AGTGGCATGG CCGTCTCAAG AAGGACAGAA TCCCAGCCTC
    TCTTCCATCA GAAAATTGAT TAGAGATGGC AGCATTGACC TAGTGATTAA CCTTCCCAAC
    AACAACACTA AATTTGTCCA TGATAATTAT GTGATTCGGA GGACAGCTGT TGATAGTGGA
    ATCCCTCTCC TCACTAATTT TCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT
    CGCAAGGTGG ACTCCAAGAG TCTTTTCCAC TACAGGCAGT ACAGTGCTGG AAAAGCAGCA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57055
    Clone ID Related Accession (Same CDS sequence) NM_001369256.1 , XM_011510640.1
    Accession Version NM_001369256.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-16
    Organism Homo sapiens(human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007970.3 and AC008172.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.27591.1, SRR1660803.123996.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAAATGT TGGAAGAGCC ACAAATCATC AAAATGACGA GGATTTTGAC AGCTTTCAAA 
    GTGGTGAGGA CACTGAAGAC TGGTTTTGGC TTTACCAATG TGACTGCACA CCAAAAATGG
    AAATTTTCAA GACCTGGCAT CAGGCTCCTT TCTGTCAAGG CACAGACAGC ACACATTGTC
    CTGGAAGATG GAACTAAGAT GAAAGGTTAC TCCTTTGGCC ATCCATCCTC TGTTGCTGGT
    GAAGTGGTTT TTAATACTGG CCTGGGAGGG TACCCAGAAG CTATTACTGA CCCTGCCTAC
    AAAGGACAGA TTCTCACAAT GGCCAACCCT ATTATTGGGA ATGGTGGAGC TCCTGATACT
    ACTGCTCTGG ATGAACTGGG ACTTAGCAAA TATTTGGAGT CTAATGGAAT CAAGGTTTCA
    GGTTTGCTGG TGCTGGATTA TAGTAAAGAC TACAACCACT GGCTGGCTAC CAAGAGTTTA
    GGGCAATGGC TACAGGAAGA AAAGGTTCCT GCAATTTATG GAGTGGACAC AAGAATGCTG
    ACTAAAATAA TTCGGGATAA GGGTACCATG CTTGGGAAGA TTGAATTTGA AGGTCAGCCT
    GTGGATTTTG TGGATCCAAA TAAACAGAAT TTGATTGCTG AGGTTTCAAC CAAGGATGTC
    AAAGTGTACG GCAAAGGAAA CCCCACAAAA GTGGTAGCTG TAGACTGTGG GATTAAAAAC
    AATGTAATCC GCCTGCTAGT AAAGCGAGGA GCTGAAGTGC ACTTAGTTCC CTGGAACCAT
    GATTTCACCA AGATGGAGTA TGATGGGATT TTGATCGCGG GAGGACCGGG GAACCCAGCT
    CTTGCAGAAC CACTAATTCA GAATGTCAGA AAGATTTTGG AGAGTGATCG CAAGGAGCCA
    TTGTTTGGAA TCAGTACAGG AAACTTAATA ACAGGATTGG CTGCTGGTGC CAAAACCTAC
    AAGATGTCCA TGGCCAACAG AGGGCAGAAT CAGCCTGTTT TGAATATCAC AAACAAACAG
    GCTTTCATTA CTGCTCAGAA TCATGGCTAT GCCTTGGACA ACACCCTCCC TGCTGGCTGG
    AAACCACTTT TTGTGAATGT CAACGATCAA ACAAATGAGG GGATTATGCA TGAGAGCAAA
    CCCTTCTTCG CTGTGCAGTT CCACCCAGAG GTCACCCCGG GGCCAATAGA CACTGAGTAC
    CTGTTTGATT CCTTTTTCTC ACTGATAAAG AAAGGAAAAG CTACCACCAT TACATCAGTC
    TTACCGAAGC CAGCACTAGT TGCATCTCGG GTTGAGGTTT CCAAAGTCCT TATTCTAGGA
    TCAGGAGGTC TGTCCATTGG TCAGGCTGGA GAATTTGATT ACTCAGGATC TCAAGCTGTA
    AAAGCCATGA AGGAAGAAAA TGTCAAAACT GTTCTGATGA ACCCAAACAT TGCATCAGTC
    CAGACCAATG AGGTGGGCTT AAAGCAAGCG GATACTGTCT ACTTTCTTCC CATCACCCCT
    CAGTTTGTCA CAGAGGTCAT CAAGGCAGAA CAGCCAGATG GGTTAATTCT GGGCATGGGT
    GGCCAGACAG CTCTGAACTG TGGAGTGGAA CTATTCAAGA GAGGTGTGCT CAAGGAATAT
    GGTGTGAAAG TCCTGGGAAC TTCAGTTGAG TCCATTATGG CTACGGAAGA CAGGCAGCTG
    TTTTCAGATA AACTAAATGA GATCAATGAA AAGATTGCTC CAAGTTTTGC AGTGGAATCG
    ATTGAGGATG CACTGAAGGC AGCAGACACC ATTGGCTACC CAGTGATGAT CCGTTCCGCC
    TATGCACTGG GTGGGTTAGG CTCAGGCATC TGTCCCAACA GAGAGACTTT GATGGACCTC
    AGCACAAAGG CCTTTGCTAT GACCAACCAA ATTCTGGTGG AGAAGTCAGT GACAGGTTGG
    AAAGAAATAG AATATGAAGT GGTTCGAGAT GCTGATGACA ATTGTGTCAC TGTCTGTAAC
    ATGGAAAATG TTGATGCCAT GGGTGTTCAC ACAGGTGACT CAGTTGTTGT GGCTCCTGCC
    CAGACACTCT CCAATGCCGA GTTTCAGATG TTGAGACGTA CTTCAATCAA TGTTGTTCGC
    CACTTGGGCA TTGTGGGTGA ATGCAACATT CAGTTTGCCC TTCATCCTAC CTCAATGGAA
    TACTGCATCA TTGAAGTGAA TGCCAGACTG TCCCGAAGCT CTGCTCTGGC CTCAAAAGCC
    ACTGGCTACC CATTGGCATT CATTGCTGCA AAGATTGCCC TAGGAATCCC ACTTCCAGAA
    ATTAAGAACG TCGTATCCGG GAAGACATCA GCCTGTTTTG AACCTAGCCT GGATTACATG
    GTCACCAAGA TTCCCCGCTG GGATCTTGAC CGTTTTCATG GAACATCTAG CCGAATTGGT
    AGCTCTATGA AAAGTGTAGG AGAGGTCATG GCTATTGGTC GTACCTTTGA GGAGAGTTTC
    CAGAAAGCTT TACGGATGTG CCACCCATCT ATAGAAGGTT TCACTCCCCG TCTCCCAATG
    AACAAAGAAT GGCCATCTAA TTTAGATCTT AGAAAAGAGT TGTCTGAACC AAGCAGCACG
    CGTATCTATG CCATTGCCAA GGCCATTGAT GACAACATGT CCCTTGATGA GATTGAGAAG
    CTCACATACA TTGACAAGTG GTTTTTGTAT AAGATGCGTG ATATTTTAAA CATGGAAAAG
    ACACTGAAAG GCCTCAACAG TGAGTCCATG ACAGAAGAAA CCCTGAAAAG GGCAAAGGAG
    ATTGGGTTCT CAGATAAGCA GATTTCAAAA TGCCTTGGGC TCACTGAGGC CCAGACAAGG
    GAGCTGAGGT TAAAGAAAAA CATCCACCCT TGGGTTAAAC AGATTGATAC ACTGGCTGCA
    GAATACCCAT CAGTAACAAA CTATCTCTAT GTTACCTACA ATGGTCAGGA GCATGATGTC
    AATTTTGATG ACCATGGAAT GATGGTGCTA GGCTGTGGTC CATATCACAT TGGCAGCAGT
    GTGGAATTTG ATTGGTGTGC TGTCTCTAGT ATCCGCACAC TGCGTCAACT TGGCAAGAAG
    ACGGTGGTGG TGAATTGCAA TCCTGAGACT GTGAGCACAG ACTTTGATGA GTGTGACAAA
    CTGTACTTTG AAGAGTTGTC CTTGGAGAGA ATCCTAGACA TCTACCATCA GGAGGCATGT
    GGTGGCTGCA TCATATCAGT TGGAGGCCAG ATTCCAAACA ACCTGGCAGT TCCTCTATAC
    AAGAATGGTG TCAAGATCAT GGGCACAAGC CCCCTGCAGA TCGACAGGGC TGAGGATCGC
    TCCATCTTCT CAGCTGTCTT GGATGAGCTG AAGGTGGCTC AGGCACCTTG GAAAGCTGTT
    AATACTTTGA ATGAAGCACT GGAATTTGCA AAGTCTGTGG ACTACCCCTG CTTGTTGAGG
    CCTTCCTATG TTTTGAGTGG GTCTGCTATG AATGTGGTAT TCTCTGAGGA TGAGATGAAA
    AAATTCCTAG AAGAGGCGAC TAGAGTTTCT CAGGAGCACC CAGTGGTGCT GACAAAATTT
    GTTGAAGGGG CCCGAGAAGT AGAAATGGAC GCTGTTGGCA AAGATGGAAG GGTTATCTCT
    CATGCCATCT CTGAACATGT TGAAGATGCA GGTGTCCACT CGGGAGATGC CACTCTGATG
    CTGCCCACAC AAACCATCAG CCAAGGGGCC ATTGAAAAGG TGAAGGATGC TACCCGGAAG
    ATTGCAAAGG CTTTTGCCAT CTCTGGTCCA TTCAACGTCC AATTTCTTGT CAAAGGAAAT
    GATGTCTTGG TGATTGAGTG TAACTTGAGA GCTTCTCGAT CCTTCCCCTT TGTTTCCAAG
    ACTCTTGGGG TTGACTTCAT TGATGTGGCC ACCAAGGTGA TGATTGGAGA GAATGTTGAT
    GAGAAACATC TTCCAACATT GGACCATCCC ATAATTCCTG CTGACTATGT TGCAATTAAG
    GCTCCCATGT TTTCCTGGCC CCGGTTGAGG GATGCTGACC CCATTCTGAG ATGTGAGATG
    GCTTCCACTG GAGAGGTGGC TTGCTTTGGT GAAGGTATTC ATACAGCCTT CCTAAAGGCA
    ATGCTTTCCA CAGGATTTAA GATACCCCAG AAAGGCATCC TGATAGGCAT CCAGCAATCA
    TTCCGGCCAA GATTCCTTGG TGTGGCTGAA CAATTACACA ATGAAGGTTT CAAGCTGTTT
    GCCACGGAAG CCACATCAGA CTGGCTCAAC GCCAACAATG TCCCTGCCAC CCCAGTGGCA
    TGGCCGTCTC AAGAAGGACA GAATCCCAGC CTCTCTTCCA TCAGAAAATT GATTAGAGAT
    GGCAGCATTG ACCTAGTGAT TAACCTTCCC AACAACAACA CTAAATTTGT CCATGATAAT
    TATGTGATTC GGAGGACAGC TGTTGATAGT GGAATCCCTC TCCTCACTAA TTTTCAGGTG
    ACCAAACTTT TTGCTGAAGC TGTGCAGAAA TCTCGCAAGG TGGACTCCAA GAGTCTTTTC
    CACTACAGGC AGTACAGTGC TGGAAAAGCA GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001356185.1
    CDS50..4585
    Translation

    Target ORF information:

    RefSeq Version NM_001369256.1
    Organism Homo sapiens(human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001369256.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAAATGT TGGAAGAGCC ACAAATCATC AAAATGACGA GGATTTTGAC AGCTTTCAAA 
    GTGGTGAGGA CACTGAAGAC TGGTTTTGGC TTTACCAATG TGACTGCACA CCAAAAATGG
    AAATTTTCAA GACCTGGCAT CAGGCTCCTT TCTGTCAAGG CACAGACAGC ACACATTGTC
    CTGGAAGATG GAACTAAGAT GAAAGGTTAC TCCTTTGGCC ATCCATCCTC TGTTGCTGGT
    GAAGTGGTTT TTAATACTGG CCTGGGAGGG TACCCAGAAG CTATTACTGA CCCTGCCTAC
    AAAGGACAGA TTCTCACAAT GGCCAACCCT ATTATTGGGA ATGGTGGAGC TCCTGATACT
    ACTGCTCTGG ATGAACTGGG ACTTAGCAAA TATTTGGAGT CTAATGGAAT CAAGGTTTCA
    GGTTTGCTGG TGCTGGATTA TAGTAAAGAC TACAACCACT GGCTGGCTAC CAAGAGTTTA
    GGGCAATGGC TACAGGAAGA AAAGGTTCCT GCAATTTATG GAGTGGACAC AAGAATGCTG
    ACTAAAATAA TTCGGGATAA GGGTACCATG CTTGGGAAGA TTGAATTTGA AGGTCAGCCT
    GTGGATTTTG TGGATCCAAA TAAACAGAAT TTGATTGCTG AGGTTTCAAC CAAGGATGTC
    AAAGTGTACG GCAAAGGAAA CCCCACAAAA GTGGTAGCTG TAGACTGTGG GATTAAAAAC
    AATGTAATCC GCCTGCTAGT AAAGCGAGGA GCTGAAGTGC ACTTAGTTCC CTGGAACCAT
    GATTTCACCA AGATGGAGTA TGATGGGATT TTGATCGCGG GAGGACCGGG GAACCCAGCT
    CTTGCAGAAC CACTAATTCA GAATGTCAGA AAGATTTTGG AGAGTGATCG CAAGGAGCCA
    TTGTTTGGAA TCAGTACAGG AAACTTAATA ACAGGATTGG CTGCTGGTGC CAAAACCTAC
    AAGATGTCCA TGGCCAACAG AGGGCAGAAT CAGCCTGTTT TGAATATCAC AAACAAACAG
    GCTTTCATTA CTGCTCAGAA TCATGGCTAT GCCTTGGACA ACACCCTCCC TGCTGGCTGG
    AAACCACTTT TTGTGAATGT CAACGATCAA ACAAATGAGG GGATTATGCA TGAGAGCAAA
    CCCTTCTTCG CTGTGCAGTT CCACCCAGAG GTCACCCCGG GGCCAATAGA CACTGAGTAC
    CTGTTTGATT CCTTTTTCTC ACTGATAAAG AAAGGAAAAG CTACCACCAT TACATCAGTC
    TTACCGAAGC CAGCACTAGT TGCATCTCGG GTTGAGGTTT CCAAAGTCCT TATTCTAGGA
    TCAGGAGGTC TGTCCATTGG TCAGGCTGGA GAATTTGATT ACTCAGGATC TCAAGCTGTA
    AAAGCCATGA AGGAAGAAAA TGTCAAAACT GTTCTGATGA ACCCAAACAT TGCATCAGTC
    CAGACCAATG AGGTGGGCTT AAAGCAAGCG GATACTGTCT ACTTTCTTCC CATCACCCCT
    CAGTTTGTCA CAGAGGTCAT CAAGGCAGAA CAGCCAGATG GGTTAATTCT GGGCATGGGT
    GGCCAGACAG CTCTGAACTG TGGAGTGGAA CTATTCAAGA GAGGTGTGCT CAAGGAATAT
    GGTGTGAAAG TCCTGGGAAC TTCAGTTGAG TCCATTATGG CTACGGAAGA CAGGCAGCTG
    TTTTCAGATA AACTAAATGA GATCAATGAA AAGATTGCTC CAAGTTTTGC AGTGGAATCG
    ATTGAGGATG CACTGAAGGC AGCAGACACC ATTGGCTACC CAGTGATGAT CCGTTCCGCC
    TATGCACTGG GTGGGTTAGG CTCAGGCATC TGTCCCAACA GAGAGACTTT GATGGACCTC
    AGCACAAAGG CCTTTGCTAT GACCAACCAA ATTCTGGTGG AGAAGTCAGT GACAGGTTGG
    AAAGAAATAG AATATGAAGT GGTTCGAGAT GCTGATGACA ATTGTGTCAC TGTCTGTAAC
    ATGGAAAATG TTGATGCCAT GGGTGTTCAC ACAGGTGACT CAGTTGTTGT GGCTCCTGCC
    CAGACACTCT CCAATGCCGA GTTTCAGATG TTGAGACGTA CTTCAATCAA TGTTGTTCGC
    CACTTGGGCA TTGTGGGTGA ATGCAACATT CAGTTTGCCC TTCATCCTAC CTCAATGGAA
    TACTGCATCA TTGAAGTGAA TGCCAGACTG TCCCGAAGCT CTGCTCTGGC CTCAAAAGCC
    ACTGGCTACC CATTGGCATT CATTGCTGCA AAGATTGCCC TAGGAATCCC ACTTCCAGAA
    ATTAAGAACG TCGTATCCGG GAAGACATCA GCCTGTTTTG AACCTAGCCT GGATTACATG
    GTCACCAAGA TTCCCCGCTG GGATCTTGAC CGTTTTCATG GAACATCTAG CCGAATTGGT
    AGCTCTATGA AAAGTGTAGG AGAGGTCATG GCTATTGGTC GTACCTTTGA GGAGAGTTTC
    CAGAAAGCTT TACGGATGTG CCACCCATCT ATAGAAGGTT TCACTCCCCG TCTCCCAATG
    AACAAAGAAT GGCCATCTAA TTTAGATCTT AGAAAAGAGT TGTCTGAACC AAGCAGCACG
    CGTATCTATG CCATTGCCAA GGCCATTGAT GACAACATGT CCCTTGATGA GATTGAGAAG
    CTCACATACA TTGACAAGTG GTTTTTGTAT AAGATGCGTG ATATTTTAAA CATGGAAAAG
    ACACTGAAAG GCCTCAACAG TGAGTCCATG ACAGAAGAAA CCCTGAAAAG GGCAAAGGAG
    ATTGGGTTCT CAGATAAGCA GATTTCAAAA TGCCTTGGGC TCACTGAGGC CCAGACAAGG
    GAGCTGAGGT TAAAGAAAAA CATCCACCCT TGGGTTAAAC AGATTGATAC ACTGGCTGCA
    GAATACCCAT CAGTAACAAA CTATCTCTAT GTTACCTACA ATGGTCAGGA GCATGATGTC
    AATTTTGATG ACCATGGAAT GATGGTGCTA GGCTGTGGTC CATATCACAT TGGCAGCAGT
    GTGGAATTTG ATTGGTGTGC TGTCTCTAGT ATCCGCACAC TGCGTCAACT TGGCAAGAAG
    ACGGTGGTGG TGAATTGCAA TCCTGAGACT GTGAGCACAG ACTTTGATGA GTGTGACAAA
    CTGTACTTTG AAGAGTTGTC CTTGGAGAGA ATCCTAGACA TCTACCATCA GGAGGCATGT
    GGTGGCTGCA TCATATCAGT TGGAGGCCAG ATTCCAAACA ACCTGGCAGT TCCTCTATAC
    AAGAATGGTG TCAAGATCAT GGGCACAAGC CCCCTGCAGA TCGACAGGGC TGAGGATCGC
    TCCATCTTCT CAGCTGTCTT GGATGAGCTG AAGGTGGCTC AGGCACCTTG GAAAGCTGTT
    AATACTTTGA ATGAAGCACT GGAATTTGCA AAGTCTGTGG ACTACCCCTG CTTGTTGAGG
    CCTTCCTATG TTTTGAGTGG GTCTGCTATG AATGTGGTAT TCTCTGAGGA TGAGATGAAA
    AAATTCCTAG AAGAGGCGAC TAGAGTTTCT CAGGAGCACC CAGTGGTGCT GACAAAATTT
    GTTGAAGGGG CCCGAGAAGT AGAAATGGAC GCTGTTGGCA AAGATGGAAG GGTTATCTCT
    CATGCCATCT CTGAACATGT TGAAGATGCA GGTGTCCACT CGGGAGATGC CACTCTGATG
    CTGCCCACAC AAACCATCAG CCAAGGGGCC ATTGAAAAGG TGAAGGATGC TACCCGGAAG
    ATTGCAAAGG CTTTTGCCAT CTCTGGTCCA TTCAACGTCC AATTTCTTGT CAAAGGAAAT
    GATGTCTTGG TGATTGAGTG TAACTTGAGA GCTTCTCGAT CCTTCCCCTT TGTTTCCAAG
    ACTCTTGGGG TTGACTTCAT TGATGTGGCC ACCAAGGTGA TGATTGGAGA GAATGTTGAT
    GAGAAACATC TTCCAACATT GGACCATCCC ATAATTCCTG CTGACTATGT TGCAATTAAG
    GCTCCCATGT TTTCCTGGCC CCGGTTGAGG GATGCTGACC CCATTCTGAG ATGTGAGATG
    GCTTCCACTG GAGAGGTGGC TTGCTTTGGT GAAGGTATTC ATACAGCCTT CCTAAAGGCA
    ATGCTTTCCA CAGGATTTAA GATACCCCAG AAAGGCATCC TGATAGGCAT CCAGCAATCA
    TTCCGGCCAA GATTCCTTGG TGTGGCTGAA CAATTACACA ATGAAGGTTT CAAGCTGTTT
    GCCACGGAAG CCACATCAGA CTGGCTCAAC GCCAACAATG TCCCTGCCAC CCCAGTGGCA
    TGGCCGTCTC AAGAAGGACA GAATCCCAGC CTCTCTTCCA TCAGAAAATT GATTAGAGAT
    GGCAGCATTG ACCTAGTGAT TAACCTTCCC AACAACAACA CTAAATTTGT CCATGATAAT
    TATGTGATTC GGAGGACAGC TGTTGATAGT GGAATCCCTC TCCTCACTAA TTTTCAGGTG
    ACCAAACTTT TTGCTGAAGC TGTGCAGAAA TCTCGCAAGG TGGACTCCAA GAGTCTTTTC
    CACTACAGGC AGTACAGTGC TGGAAAAGCA GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57055
    Clone ID Related Accession (Same CDS sequence) NM_001369256.1 , XM_011510640.1
    Accession Version XM_011510640.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAAATGT TGGAAGAGCC ACAAATCATC AAAATGACGA GGATTTTGAC AGCTTTCAAA 
    GTGGTGAGGA CACTGAAGAC TGGTTTTGGC TTTACCAATG TGACTGCACA CCAAAAATGG
    AAATTTTCAA GACCTGGCAT CAGGCTCCTT TCTGTCAAGG CACAGACAGC ACACATTGTC
    CTGGAAGATG GAACTAAGAT GAAAGGTTAC TCCTTTGGCC ATCCATCCTC TGTTGCTGGT
    GAAGTGGTTT TTAATACTGG CCTGGGAGGG TACCCAGAAG CTATTACTGA CCCTGCCTAC
    AAAGGACAGA TTCTCACAAT GGCCAACCCT ATTATTGGGA ATGGTGGAGC TCCTGATACT
    ACTGCTCTGG ATGAACTGGG ACTTAGCAAA TATTTGGAGT CTAATGGAAT CAAGGTTTCA
    GGTTTGCTGG TGCTGGATTA TAGTAAAGAC TACAACCACT GGCTGGCTAC CAAGAGTTTA
    GGGCAATGGC TACAGGAAGA AAAGGTTCCT GCAATTTATG GAGTGGACAC AAGAATGCTG
    ACTAAAATAA TTCGGGATAA GGGTACCATG CTTGGGAAGA TTGAATTTGA AGGTCAGCCT
    GTGGATTTTG TGGATCCAAA TAAACAGAAT TTGATTGCTG AGGTTTCAAC CAAGGATGTC
    AAAGTGTACG GCAAAGGAAA CCCCACAAAA GTGGTAGCTG TAGACTGTGG GATTAAAAAC
    AATGTAATCC GCCTGCTAGT AAAGCGAGGA GCTGAAGTGC ACTTAGTTCC CTGGAACCAT
    GATTTCACCA AGATGGAGTA TGATGGGATT TTGATCGCGG GAGGACCGGG GAACCCAGCT
    CTTGCAGAAC CACTAATTCA GAATGTCAGA AAGATTTTGG AGAGTGATCG CAAGGAGCCA
    TTGTTTGGAA TCAGTACAGG AAACTTAATA ACAGGATTGG CTGCTGGTGC CAAAACCTAC
    AAGATGTCCA TGGCCAACAG AGGGCAGAAT CAGCCTGTTT TGAATATCAC AAACAAACAG
    GCTTTCATTA CTGCTCAGAA TCATGGCTAT GCCTTGGACA ACACCCTCCC TGCTGGCTGG
    AAACCACTTT TTGTGAATGT CAACGATCAA ACAAATGAGG GGATTATGCA TGAGAGCAAA
    CCCTTCTTCG CTGTGCAGTT CCACCCAGAG GTCACCCCGG GGCCAATAGA CACTGAGTAC
    CTGTTTGATT CCTTTTTCTC ACTGATAAAG AAAGGAAAAG CTACCACCAT TACATCAGTC
    TTACCGAAGC CAGCACTAGT TGCATCTCGG GTTGAGGTTT CCAAAGTCCT TATTCTAGGA
    TCAGGAGGTC TGTCCATTGG TCAGGCTGGA GAATTTGATT ACTCAGGATC TCAAGCTGTA
    AAAGCCATGA AGGAAGAAAA TGTCAAAACT GTTCTGATGA ACCCAAACAT TGCATCAGTC
    CAGACCAATG AGGTGGGCTT AAAGCAAGCG GATACTGTCT ACTTTCTTCC CATCACCCCT
    CAGTTTGTCA CAGAGGTCAT CAAGGCAGAA CAGCCAGATG GGTTAATTCT GGGCATGGGT
    GGCCAGACAG CTCTGAACTG TGGAGTGGAA CTATTCAAGA GAGGTGTGCT CAAGGAATAT
    GGTGTGAAAG TCCTGGGAAC TTCAGTTGAG TCCATTATGG CTACGGAAGA CAGGCAGCTG
    TTTTCAGATA AACTAAATGA GATCAATGAA AAGATTGCTC CAAGTTTTGC AGTGGAATCG
    ATTGAGGATG CACTGAAGGC AGCAGACACC ATTGGCTACC CAGTGATGAT CCGTTCCGCC
    TATGCACTGG GTGGGTTAGG CTCAGGCATC TGTCCCAACA GAGAGACTTT GATGGACCTC
    AGCACAAAGG CCTTTGCTAT GACCAACCAA ATTCTGGTGG AGAAGTCAGT GACAGGTTGG
    AAAGAAATAG AATATGAAGT GGTTCGAGAT GCTGATGACA ATTGTGTCAC TGTCTGTAAC
    ATGGAAAATG TTGATGCCAT GGGTGTTCAC ACAGGTGACT CAGTTGTTGT GGCTCCTGCC
    CAGACACTCT CCAATGCCGA GTTTCAGATG TTGAGACGTA CTTCAATCAA TGTTGTTCGC
    CACTTGGGCA TTGTGGGTGA ATGCAACATT CAGTTTGCCC TTCATCCTAC CTCAATGGAA
    TACTGCATCA TTGAAGTGAA TGCCAGACTG TCCCGAAGCT CTGCTCTGGC CTCAAAAGCC
    ACTGGCTACC CATTGGCATT CATTGCTGCA AAGATTGCCC TAGGAATCCC ACTTCCAGAA
    ATTAAGAACG TCGTATCCGG GAAGACATCA GCCTGTTTTG AACCTAGCCT GGATTACATG
    GTCACCAAGA TTCCCCGCTG GGATCTTGAC CGTTTTCATG GAACATCTAG CCGAATTGGT
    AGCTCTATGA AAAGTGTAGG AGAGGTCATG GCTATTGGTC GTACCTTTGA GGAGAGTTTC
    CAGAAAGCTT TACGGATGTG CCACCCATCT ATAGAAGGTT TCACTCCCCG TCTCCCAATG
    AACAAAGAAT GGCCATCTAA TTTAGATCTT AGAAAAGAGT TGTCTGAACC AAGCAGCACG
    CGTATCTATG CCATTGCCAA GGCCATTGAT GACAACATGT CCCTTGATGA GATTGAGAAG
    CTCACATACA TTGACAAGTG GTTTTTGTAT AAGATGCGTG ATATTTTAAA CATGGAAAAG
    ACACTGAAAG GCCTCAACAG TGAGTCCATG ACAGAAGAAA CCCTGAAAAG GGCAAAGGAG
    ATTGGGTTCT CAGATAAGCA GATTTCAAAA TGCCTTGGGC TCACTGAGGC CCAGACAAGG
    GAGCTGAGGT TAAAGAAAAA CATCCACCCT TGGGTTAAAC AGATTGATAC ACTGGCTGCA
    GAATACCCAT CAGTAACAAA CTATCTCTAT GTTACCTACA ATGGTCAGGA GCATGATGTC
    AATTTTGATG ACCATGGAAT GATGGTGCTA GGCTGTGGTC CATATCACAT TGGCAGCAGT
    GTGGAATTTG ATTGGTGTGC TGTCTCTAGT ATCCGCACAC TGCGTCAACT TGGCAAGAAG
    ACGGTGGTGG TGAATTGCAA TCCTGAGACT GTGAGCACAG ACTTTGATGA GTGTGACAAA
    CTGTACTTTG AAGAGTTGTC CTTGGAGAGA ATCCTAGACA TCTACCATCA GGAGGCATGT
    GGTGGCTGCA TCATATCAGT TGGAGGCCAG ATTCCAAACA ACCTGGCAGT TCCTCTATAC
    AAGAATGGTG TCAAGATCAT GGGCACAAGC CCCCTGCAGA TCGACAGGGC TGAGGATCGC
    TCCATCTTCT CAGCTGTCTT GGATGAGCTG AAGGTGGCTC AGGCACCTTG GAAAGCTGTT
    AATACTTTGA ATGAAGCACT GGAATTTGCA AAGTCTGTGG ACTACCCCTG CTTGTTGAGG
    CCTTCCTATG TTTTGAGTGG GTCTGCTATG AATGTGGTAT TCTCTGAGGA TGAGATGAAA
    AAATTCCTAG AAGAGGCGAC TAGAGTTTCT CAGGAGCACC CAGTGGTGCT GACAAAATTT
    GTTGAAGGGG CCCGAGAAGT AGAAATGGAC GCTGTTGGCA AAGATGGAAG GGTTATCTCT
    CATGCCATCT CTGAACATGT TGAAGATGCA GGTGTCCACT CGGGAGATGC CACTCTGATG
    CTGCCCACAC AAACCATCAG CCAAGGGGCC ATTGAAAAGG TGAAGGATGC TACCCGGAAG
    ATTGCAAAGG CTTTTGCCAT CTCTGGTCCA TTCAACGTCC AATTTCTTGT CAAAGGAAAT
    GATGTCTTGG TGATTGAGTG TAACTTGAGA GCTTCTCGAT CCTTCCCCTT TGTTTCCAAG
    ACTCTTGGGG TTGACTTCAT TGATGTGGCC ACCAAGGTGA TGATTGGAGA GAATGTTGAT
    GAGAAACATC TTCCAACATT GGACCATCCC ATAATTCCTG CTGACTATGT TGCAATTAAG
    GCTCCCATGT TTTCCTGGCC CCGGTTGAGG GATGCTGACC CCATTCTGAG ATGTGAGATG
    GCTTCCACTG GAGAGGTGGC TTGCTTTGGT GAAGGTATTC ATACAGCCTT CCTAAAGGCA
    ATGCTTTCCA CAGGATTTAA GATACCCCAG AAAGGCATCC TGATAGGCAT CCAGCAATCA
    TTCCGGCCAA GATTCCTTGG TGTGGCTGAA CAATTACACA ATGAAGGTTT CAAGCTGTTT
    GCCACGGAAG CCACATCAGA CTGGCTCAAC GCCAACAATG TCCCTGCCAC CCCAGTGGCA
    TGGCCGTCTC AAGAAGGACA GAATCCCAGC CTCTCTTCCA TCAGAAAATT GATTAGAGAT
    GGCAGCATTG ACCTAGTGAT TAACCTTCCC AACAACAACA CTAAATTTGT CCATGATAAT
    TATGTGATTC GGAGGACAGC TGTTGATAGT GGAATCCCTC TCCTCACTAA TTTTCAGGTG
    ACCAAACTTT TTGCTGAAGC TGTGCAGAAA TCTCGCAAGG TGGACTCCAA GAGTCTTTTC
    CACTACAGGC AGTACAGTGC TGGAAAAGCA GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508942.1
    CDS302..4837
    Translation

    Target ORF information:

    RefSeq Version XM_011510640.1
    Organism Homo sapiens(human)
    Definition Homo sapiens carbamoyl-phosphate synthase 1 (CPS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAAATGT TGGAAGAGCC ACAAATCATC AAAATGACGA GGATTTTGAC AGCTTTCAAA 
    GTGGTGAGGA CACTGAAGAC TGGTTTTGGC TTTACCAATG TGACTGCACA CCAAAAATGG
    AAATTTTCAA GACCTGGCAT CAGGCTCCTT TCTGTCAAGG CACAGACAGC ACACATTGTC
    CTGGAAGATG GAACTAAGAT GAAAGGTTAC TCCTTTGGCC ATCCATCCTC TGTTGCTGGT
    GAAGTGGTTT TTAATACTGG CCTGGGAGGG TACCCAGAAG CTATTACTGA CCCTGCCTAC
    AAAGGACAGA TTCTCACAAT GGCCAACCCT ATTATTGGGA ATGGTGGAGC TCCTGATACT
    ACTGCTCTGG ATGAACTGGG ACTTAGCAAA TATTTGGAGT CTAATGGAAT CAAGGTTTCA
    GGTTTGCTGG TGCTGGATTA TAGTAAAGAC TACAACCACT GGCTGGCTAC CAAGAGTTTA
    GGGCAATGGC TACAGGAAGA AAAGGTTCCT GCAATTTATG GAGTGGACAC AAGAATGCTG
    ACTAAAATAA TTCGGGATAA GGGTACCATG CTTGGGAAGA TTGAATTTGA AGGTCAGCCT
    GTGGATTTTG TGGATCCAAA TAAACAGAAT TTGATTGCTG AGGTTTCAAC CAAGGATGTC
    AAAGTGTACG GCAAAGGAAA CCCCACAAAA GTGGTAGCTG TAGACTGTGG GATTAAAAAC
    AATGTAATCC GCCTGCTAGT AAAGCGAGGA GCTGAAGTGC ACTTAGTTCC CTGGAACCAT
    GATTTCACCA AGATGGAGTA TGATGGGATT TTGATCGCGG GAGGACCGGG GAACCCAGCT
    CTTGCAGAAC CACTAATTCA GAATGTCAGA AAGATTTTGG AGAGTGATCG CAAGGAGCCA
    TTGTTTGGAA TCAGTACAGG AAACTTAATA ACAGGATTGG CTGCTGGTGC CAAAACCTAC
    AAGATGTCCA TGGCCAACAG AGGGCAGAAT CAGCCTGTTT TGAATATCAC AAACAAACAG
    GCTTTCATTA CTGCTCAGAA TCATGGCTAT GCCTTGGACA ACACCCTCCC TGCTGGCTGG
    AAACCACTTT TTGTGAATGT CAACGATCAA ACAAATGAGG GGATTATGCA TGAGAGCAAA
    CCCTTCTTCG CTGTGCAGTT CCACCCAGAG GTCACCCCGG GGCCAATAGA CACTGAGTAC
    CTGTTTGATT CCTTTTTCTC ACTGATAAAG AAAGGAAAAG CTACCACCAT TACATCAGTC
    TTACCGAAGC CAGCACTAGT TGCATCTCGG GTTGAGGTTT CCAAAGTCCT TATTCTAGGA
    TCAGGAGGTC TGTCCATTGG TCAGGCTGGA GAATTTGATT ACTCAGGATC TCAAGCTGTA
    AAAGCCATGA AGGAAGAAAA TGTCAAAACT GTTCTGATGA ACCCAAACAT TGCATCAGTC
    CAGACCAATG AGGTGGGCTT AAAGCAAGCG GATACTGTCT ACTTTCTTCC CATCACCCCT
    CAGTTTGTCA CAGAGGTCAT CAAGGCAGAA CAGCCAGATG GGTTAATTCT GGGCATGGGT
    GGCCAGACAG CTCTGAACTG TGGAGTGGAA CTATTCAAGA GAGGTGTGCT CAAGGAATAT
    GGTGTGAAAG TCCTGGGAAC TTCAGTTGAG TCCATTATGG CTACGGAAGA CAGGCAGCTG
    TTTTCAGATA AACTAAATGA GATCAATGAA AAGATTGCTC CAAGTTTTGC AGTGGAATCG
    ATTGAGGATG CACTGAAGGC AGCAGACACC ATTGGCTACC CAGTGATGAT CCGTTCCGCC
    TATGCACTGG GTGGGTTAGG CTCAGGCATC TGTCCCAACA GAGAGACTTT GATGGACCTC
    AGCACAAAGG CCTTTGCTAT GACCAACCAA ATTCTGGTGG AGAAGTCAGT GACAGGTTGG
    AAAGAAATAG AATATGAAGT GGTTCGAGAT GCTGATGACA ATTGTGTCAC TGTCTGTAAC
    ATGGAAAATG TTGATGCCAT GGGTGTTCAC ACAGGTGACT CAGTTGTTGT GGCTCCTGCC
    CAGACACTCT CCAATGCCGA GTTTCAGATG TTGAGACGTA CTTCAATCAA TGTTGTTCGC
    CACTTGGGCA TTGTGGGTGA ATGCAACATT CAGTTTGCCC TTCATCCTAC CTCAATGGAA
    TACTGCATCA TTGAAGTGAA TGCCAGACTG TCCCGAAGCT CTGCTCTGGC CTCAAAAGCC
    ACTGGCTACC CATTGGCATT CATTGCTGCA AAGATTGCCC TAGGAATCCC ACTTCCAGAA
    ATTAAGAACG TCGTATCCGG GAAGACATCA GCCTGTTTTG AACCTAGCCT GGATTACATG
    GTCACCAAGA TTCCCCGCTG GGATCTTGAC CGTTTTCATG GAACATCTAG CCGAATTGGT
    AGCTCTATGA AAAGTGTAGG AGAGGTCATG GCTATTGGTC GTACCTTTGA GGAGAGTTTC
    CAGAAAGCTT TACGGATGTG CCACCCATCT ATAGAAGGTT TCACTCCCCG TCTCCCAATG
    AACAAAGAAT GGCCATCTAA TTTAGATCTT AGAAAAGAGT TGTCTGAACC AAGCAGCACG
    CGTATCTATG CCATTGCCAA GGCCATTGAT GACAACATGT CCCTTGATGA GATTGAGAAG
    CTCACATACA TTGACAAGTG GTTTTTGTAT AAGATGCGTG ATATTTTAAA CATGGAAAAG
    ACACTGAAAG GCCTCAACAG TGAGTCCATG ACAGAAGAAA CCCTGAAAAG GGCAAAGGAG
    ATTGGGTTCT CAGATAAGCA GATTTCAAAA TGCCTTGGGC TCACTGAGGC CCAGACAAGG
    GAGCTGAGGT TAAAGAAAAA CATCCACCCT TGGGTTAAAC AGATTGATAC ACTGGCTGCA
    GAATACCCAT CAGTAACAAA CTATCTCTAT GTTACCTACA ATGGTCAGGA GCATGATGTC
    AATTTTGATG ACCATGGAAT GATGGTGCTA GGCTGTGGTC CATATCACAT TGGCAGCAGT
    GTGGAATTTG ATTGGTGTGC TGTCTCTAGT ATCCGCACAC TGCGTCAACT TGGCAAGAAG
    ACGGTGGTGG TGAATTGCAA TCCTGAGACT GTGAGCACAG ACTTTGATGA GTGTGACAAA
    CTGTACTTTG AAGAGTTGTC CTTGGAGAGA ATCCTAGACA TCTACCATCA GGAGGCATGT
    GGTGGCTGCA TCATATCAGT TGGAGGCCAG ATTCCAAACA ACCTGGCAGT TCCTCTATAC
    AAGAATGGTG TCAAGATCAT GGGCACAAGC CCCCTGCAGA TCGACAGGGC TGAGGATCGC
    TCCATCTTCT CAGCTGTCTT GGATGAGCTG AAGGTGGCTC AGGCACCTTG GAAAGCTGTT
    AATACTTTGA ATGAAGCACT GGAATTTGCA AAGTCTGTGG ACTACCCCTG CTTGTTGAGG
    CCTTCCTATG TTTTGAGTGG GTCTGCTATG AATGTGGTAT TCTCTGAGGA TGAGATGAAA
    AAATTCCTAG AAGAGGCGAC TAGAGTTTCT CAGGAGCACC CAGTGGTGCT GACAAAATTT
    GTTGAAGGGG CCCGAGAAGT AGAAATGGAC GCTGTTGGCA AAGATGGAAG GGTTATCTCT
    CATGCCATCT CTGAACATGT TGAAGATGCA GGTGTCCACT CGGGAGATGC CACTCTGATG
    CTGCCCACAC AAACCATCAG CCAAGGGGCC ATTGAAAAGG TGAAGGATGC TACCCGGAAG
    ATTGCAAAGG CTTTTGCCAT CTCTGGTCCA TTCAACGTCC AATTTCTTGT CAAAGGAAAT
    GATGTCTTGG TGATTGAGTG TAACTTGAGA GCTTCTCGAT CCTTCCCCTT TGTTTCCAAG
    ACTCTTGGGG TTGACTTCAT TGATGTGGCC ACCAAGGTGA TGATTGGAGA GAATGTTGAT
    GAGAAACATC TTCCAACATT GGACCATCCC ATAATTCCTG CTGACTATGT TGCAATTAAG
    GCTCCCATGT TTTCCTGGCC CCGGTTGAGG GATGCTGACC CCATTCTGAG ATGTGAGATG
    GCTTCCACTG GAGAGGTGGC TTGCTTTGGT GAAGGTATTC ATACAGCCTT CCTAAAGGCA
    ATGCTTTCCA CAGGATTTAA GATACCCCAG AAAGGCATCC TGATAGGCAT CCAGCAATCA
    TTCCGGCCAA GATTCCTTGG TGTGGCTGAA CAATTACACA ATGAAGGTTT CAAGCTGTTT
    GCCACGGAAG CCACATCAGA CTGGCTCAAC GCCAACAATG TCCCTGCCAC CCCAGTGGCA
    TGGCCGTCTC AAGAAGGACA GAATCCCAGC CTCTCTTCCA TCAGAAAATT GATTAGAGAT
    GGCAGCATTG ACCTAGTGAT TAACCTTCCC AACAACAACA CTAAATTTGT CCATGATAAT
    TATGTGATTC GGAGGACAGC TGTTGATAGT GGAATCCCTC TCCTCACTAA TTTTCAGGTG
    ACCAAACTTT TTGCTGAAGC TGTGCAGAAA TCTCGCAAGG TGGACTCCAA GAGTCTTTTC
    CACTACAGGC AGTACAGTGC TGGAAAAGCA GCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.