CPE cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CPE
Entrez Gene ID	1363
Full Name	carboxypeptidase E
Synonyms	CPH
General protein information	Preferred Names carboxypeptidase E Names carboxypeptidase E carboxypeptidase H cobalt-stimulated chromaffin granule carboxypeptidase enkephalin convertase insulin granule-associated carboxypeptidase prohormone-processing carboxypeptidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q32.3
Summary	This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. [provided by RefSeq, Nov 2015].
Disorder MIM:	114855

mRNA and Protein(s)

mRNA	Protein	Name
NM_001873.2	NP_001864.1	carboxypeptidase E preproprotein
NM_001873.4	NP_001864.1	carboxypeptidase E preproprotein
NM_001873.3	NP_001864.1	carboxypeptidase E preproprotein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cpe	NP_037260.1
Xenopus tropicalis (tropical clawed frog)	cpe	NP_001106417.1
	CPE	XP_001097378.2
Danio rerio (zebrafish)	cpe	NP_999975.1
Pan troglodytes (chimpanzee)	CPE	NP_001092029.1
Caenorhabditis elegans (roundworm)	egl-21	NP_501935.1
Homo sapiens (human)	CPE	NP_001864.1
Bos taurus (cattle)	CPE	NP_776328.2
Mus musculus (house mouse)	Cpe	NP_038522.2
Canis lupus familiaris (dog)	CPE	XP_005629450.1
Gallus gallus (chicken)	CPE	XP_420392.3

Gene Ontology
Bibliography

Related articles in PubMed

Carboxypeptidase E transmits its anti-migratory function in glioma cells via transcriptional regulation of cell architecture and motility regulating factors.
Armento A, Ilina EI, Kaoma T, Muller A, Vallar L, Niclou SP, Kr?ger MA, Mittelbronn M, Naumann U
International journal of oncology51(2)702-714(2017 Aug)

A Novel Single Nucleotide T980C Polymorphism in the Human Carboxypeptidase E Gene Results in Loss of Neuroprotective Function.
Cong L, Cheng Y, Cawley NX, Murthy SR, Loh YP
PloS one12(1)e0170169(2017)

Carboxypeptidase E (CPE) inhibits the secretion and activity of Wnt3a.
Skalka N, Caspi M, Lahav-Ariel L, Loh YP, Hirschberg K, Rosin-Arbesfeld R
Oncogene35(50)6416-6428(2016 Dec)

A human carboxypeptidase E/NF-?1 gene mutation in an Alzheimer's disease patient leads to dementia and depression in mice.
Cheng Y, Cawley NX, Yanik T, Murthy SR, Liu C, Kasikci F, Abebe D, Loh YP
Translational psychiatry6(12)e973(2016 12)

Carboxypeptidase E is a prediction marker for tumor recurrence in early-stage hepatocellular carcinoma.
Huang SF, Wu HD, Chen YT, Murthy SR, Chiu YT, Chang Y, Chang IC, Yang X, Loh YP
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(7)9745-53(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we have identified a novel SNP in the CPE gene which results in the loss of its neuroprotective function in cells and may confer neurological disorders in humans
Title: A Novel Single Nucleotide T980C Polymorphism in the Human Carboxypeptidase E Gene Results in Loss of Neuroprotective Function.

CPE through its N'-terminal sequence, forms aggregates with Wnt3a and possible endoplasmic reticulum (ER) stress leading to its loss of function.
Title: Carboxypeptidase E (CPE) inhibits the secretion and activity of Wnt3a.

This study has uncovered a human CPE/NF-alpha1 gene mutation that could lead to comorbidity of dementia and depression, emphasizing the importance of this gene in cognitive function.
Title: A human carboxypeptidase E/NF-?1 gene mutation in an Alzheimer's disease patient leads to dementia and depression in mice.

High-level CPE [ carboxypeptidase E] expression was associated with a poor prognosis in early-stage cervical cancer. CPE may serve as a biomarker for predicting PLNM [ pelvic lymph node metastasis ] and survival in these patients.
Title: CPE overexpression is correlated with pelvic lymph node metastasis and poor prognosis in patients with early-stage cervical cancer.

Low carboxypeptidase E expression is associated with recurrence in early-stage hepatocellular carcinoma.
Title: Carboxypeptidase E is a prediction marker for tumor recurrence in early-stage hepatocellular carcinoma.

The following CPE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CPE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09011	NM_001873.4 Latest version!	Homo sapiens carboxypeptidase E (CPE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu09011	NM_001873.2	Homo sapiens carboxypeptidase E (CPE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu09011	NM_001873.3	Homo sapiens carboxypeptidase E (CPE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CPE ( NM_001873.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001864.1 Homo sapiens carboxypeptidase E (CPE), mRNA.

Price & Availability↑

CloneID	OHu09011
Clone ID Related Accession (Same CDS sequence)	NM_001873.2 , NM_001873.4 , NM_001873.3
Accession Version	NM_001873.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	carboxypeptidase E preproprotein
Comment

ATGGCCGGGC GAGGGGGCAG CGCGCTGCTG GCTCTGTGCG GGGCACTGGC TGCCTGCGGG 
TGGCTCCTGG GCGCCGAAGC CCAGGAGCCC GGGGCGCCCG CGGCGGGCAT GAGGCGGCGC 
CGGCGGCTGC AGCAAGAGGA CGGCATCTCC TTCGAGTACC ACCGCTACCC CGAGCTGCGC 
GAGGCGCTCG TGTCCGTGTG GCTGCAGTGC ACCGCCATCA GCAGGATTTA CACGGTGGGG 
CGCAGCTTCG AGGGCCGGGA GCTCCTGGTC ATCGAGCTGT CCGACAACCC TGGCGTCCAT 
GAGCCTGGTG AGCCTGAATT TAAATACATT GGGAATATGC ATGGGAATGA GGCTGTTGGA 
CGAGAACTGC TCATTTTCTT GGCCCAGTAC CTATGCAACG AATACCAGAA GGGGAACGAG 
ACAATTGTCA ACCTGATCCA CAGTACCCGC ATTCACATCA TGCCTTCCCT GAACCCAGAT 
GGCTTTGAGA AGGCAGCGTC TCAGCCTGGT GAACTCAAGG ACTGGTTTGT GGGTCGAAGC 
AATGCCCAGG GAATAGATCT GAACCGGAAC TTTCCAGACC TGGATAGGAT AGTGTACGTG 
AATGAGAAAG AAGGTGGTCC AAATAATCAT CTGTTGAAAA ATATGAAGAA AATTGTGGAT 
CAAAACACAA AGCTTGCTCC TGAGACCAAG GCTGTCATTC ATTGGATTAT GGATATTCCT 
TTTGTGCTTT CTGCCAATCT CCATGGAGGA GACCTTGTGG CCAATTATCC ATATGATGAG 
ACGCGGAGTG GTAGTGCTCA CGAATACAGC TCCTCCCCAG ATGACGCCAT TTTCCAAAGC 
TTGGCCCGGG CATACTCTTC TTTCAACCCG GCCATGTCTG ACCCCAATCG GCCACCATGT 
CGCAAGAATG ATGATGACAG CAGCTTTGTA GATGGAACCA CCAACGGTGG TGCTTGGTAC 
AGCGTACCTG GAGGGATGCA AGACTTCAAT TACCTTAGCA GCAACTGTTT TGAGATCACC 
GTGGAGCTTA GCTGTGAGAA GTTCCCACCT GAAGAGACTC TGAAGACCTA CTGGGAGGAT 
AACAAAAACT CCCTCATTAG CTACCTTGAG CAGATACACC GAGGAGTTAA AGGATTTGTC 
CGAGACCTTC AAGGTAACCC AATTGCGAAT GCCACCATCT CCGTGGAAGG AATAGACCAC 
GATGTTACAT CCGCAAAGGA TGGTGATTAC TGGAGATTGC TTATACCTGG AAACTATAAA 
CTTACAGCCT CAGCTCCAGG CTATCTGGCA ATAACAAAGA AAGTGGCAGT TCCTTACAGC 
CCTGCTGCTG GGGTTGATTT TGAACTGGAG TCATTTTCTG AAAGGAAAGA AGAGGAGAAG 
GAAGAATTGA TGGAATGGTG GAAAATGATG TCAGAAACTT TAAATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_001864.1

Translation

MAGRGGSALLALCGALAACGWLLGAEAQEPGAPAAGMRRRRRLQQEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNMKKIVDQNTKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGSAHEYSSSPDDAIFQSLARAYSSFNPAMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKTYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVEGIDHDVTSAKDGDYWRLLIPGNYKLTASAPGYLAITKKVAVPYSPAAGVDFELESFSERKEEEKEELMEWWKMMSETLNF

Target ORF information:

RefSeq Version	NM_001873.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens carboxypeptidase E (CPE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001873.2

ORF Insert Sequence:

ATGGCCGGGC GAGGGGGCAG CGCGCTGCTG GCTCTGTGCG GGGCACTGGC TGCCTGCGGG 
TGGCTCCTGG GCGCCGAAGC CCAGGAGCCC GGGGCGCCCG CGGCGGGCAT GAGGCGGCGC 
CGGCGGCTGC AGCAAGAGGA CGGCATCTCC TTCGAGTACC ACCGCTACCC CGAGCTGCGC 
GAGGCGCTCG TGTCCGTGTG GCTGCAGTGC ACCGCCATCA GCAGGATTTA CACGGTGGGG 
CGCAGCTTCG AGGGCCGGGA GCTCCTGGTC ATCGAGCTGT CCGACAACCC TGGCGTCCAT 
GAGCCTGGTG AGCCTGAATT TAAATACATT GGGAATATGC ATGGGAATGA GGCTGTTGGA 
CGAGAACTGC TCATTTTCTT GGCCCAGTAC CTATGCAACG AATACCAGAA GGGGAACGAG 
ACAATTGTCA ACCTGATCCA CAGTACCCGC ATTCACATCA TGCCTTCCCT GAACCCAGAT 
GGCTTTGAGA AGGCAGCGTC TCAGCCTGGT GAACTCAAGG ACTGGTTTGT GGGTCGAAGC 
AATGCCCAGG GAATAGATCT GAACCGGAAC TTTCCAGACC TGGATAGGAT AGTGTACGTG 
AATGAGAAAG AAGGTGGTCC AAATAATCAT CTGTTGAAAA ATATGAAGAA AATTGTGGAT 
CAAAACACAA AGCTTGCTCC TGAGACCAAG GCTGTCATTC ATTGGATTAT GGATATTCCT 
TTTGTGCTTT CTGCCAATCT CCATGGAGGA GACCTTGTGG CCAATTATCC ATATGATGAG 
ACGCGGAGTG GTAGTGCTCA CGAATACAGC TCCTCCCCAG ATGACGCCAT TTTCCAAAGC 
TTGGCCCGGG CATACTCTTC TTTCAACCCG GCCATGTCTG ACCCCAATCG GCCACCATGT 
CGCAAGAATG ATGATGACAG CAGCTTTGTA GATGGAACCA CCAACGGTGG TGCTTGGTAC 
AGCGTACCTG GAGGGATGCA AGACTTCAAT TACCTTAGCA GCAACTGTTT TGAGATCACC 
GTGGAGCTTA GCTGTGAGAA GTTCCCACCT GAAGAGACTC TGAAGACCTA CTGGGAGGAT 
AACAAAAACT CCCTCATTAG CTACCTTGAG CAGATACACC GAGGAGTTAA AGGATTTGTC 
CGAGACCTTC AAGGTAACCC AATTGCGAAT GCCACCATCT CCGTGGAAGG AATAGACCAC 
GATGTTACAT CCGCAAAGGA TGGTGATTAC TGGAGATTGC TTATACCTGG AAACTATAAA 
CTTACAGCCT CAGCTCCAGG CTATCTGGCA ATAACAAAGA AAGTGGCAGT TCCTTACAGC 
CCTGCTGCTG GGGTTGATTT TGAACTGGAG TCATTTTCTG AAAGGAAAGA AGAGGAGAAG 
GAAGAATTGA TGGAATGGTG GAAAATGATG TCAGAAACTT TAAATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPE ( NM_001873.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001864.1 Homo sapiens carboxypeptidase E (CPE), mRNA.

Price & Availability↑

CloneID	OHu09011
Clone ID Related Accession (Same CDS sequence)	NM_001873.2 , NM_001873.4 , NM_001873.3
Accession Version	NM_001873.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	carboxypeptidase E preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X51405.1, AA683379.1, AC012504.7 and AA883804.1. On Nov 23, 2018 this sequence version replaced NM_001873.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.83919.1, SRR3476690.1065405.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000402744.9/ ENSP00000386104.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGGGC GAGGGGGCAG CGCGCTGCTG GCTCTGTGCG GGGCACTGGC TGCCTGCGGG 
TGGCTCCTGG GCGCCGAAGC CCAGGAGCCC GGGGCGCCCG CGGCGGGCAT GAGGCGGCGC 
CGGCGGCTGC AGCAAGAGGA CGGCATCTCC TTCGAGTACC ACCGCTACCC CGAGCTGCGC 
GAGGCGCTCG TGTCCGTGTG GCTGCAGTGC ACCGCCATCA GCAGGATTTA CACGGTGGGG 
CGCAGCTTCG AGGGCCGGGA GCTCCTGGTC ATCGAGCTGT CCGACAACCC TGGCGTCCAT 
GAGCCTGGTG AGCCTGAATT TAAATACATT GGGAATATGC ATGGGAATGA GGCTGTTGGA 
CGAGAACTGC TCATTTTCTT GGCCCAGTAC CTATGCAACG AATACCAGAA GGGGAACGAG 
ACAATTGTCA ACCTGATCCA CAGTACCCGC ATTCACATCA TGCCTTCCCT GAACCCAGAT 
GGCTTTGAGA AGGCAGCGTC TCAGCCTGGT GAACTCAAGG ACTGGTTTGT GGGTCGAAGC 
AATGCCCAGG GAATAGATCT GAACCGGAAC TTTCCAGACC TGGATAGGAT AGTGTACGTG 
AATGAGAAAG AAGGTGGTCC AAATAATCAT CTGTTGAAAA ATATGAAGAA AATTGTGGAT 
CAAAACACAA AGCTTGCTCC TGAGACCAAG GCTGTCATTC ATTGGATTAT GGATATTCCT 
TTTGTGCTTT CTGCCAATCT CCATGGAGGA GACCTTGTGG CCAATTATCC ATATGATGAG 
ACGCGGAGTG GTAGTGCTCA CGAATACAGC TCCTCCCCAG ATGACGCCAT TTTCCAAAGC 
TTGGCCCGGG CATACTCTTC TTTCAACCCG GCCATGTCTG ACCCCAATCG GCCACCATGT 
CGCAAGAATG ATGATGACAG CAGCTTTGTA GATGGAACCA CCAACGGTGG TGCTTGGTAC 
AGCGTACCTG GAGGGATGCA AGACTTCAAT TACCTTAGCA GCAACTGTTT TGAGATCACC 
GTGGAGCTTA GCTGTGAGAA GTTCCCACCT GAAGAGACTC TGAAGACCTA CTGGGAGGAT 
AACAAAAACT CCCTCATTAG CTACCTTGAG CAGATACACC GAGGAGTTAA AGGATTTGTC 
CGAGACCTTC AAGGTAACCC AATTGCGAAT GCCACCATCT CCGTGGAAGG AATAGACCAC 
GATGTTACAT CCGCAAAGGA TGGTGATTAC TGGAGATTGC TTATACCTGG AAACTATAAA 
CTTACAGCCT CAGCTCCAGG CTATCTGGCA ATAACAAAGA AAGTGGCAGT TCCTTACAGC 
CCTGCTGCTG GGGTTGATTT TGAACTGGAG TCATTTTCTG AAAGGAAAGA AGAGGAGAAG 
GAAGAATTGA TGGAATGGTG GAAAATGATG TCAGAAACTT TAAATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001864.1
CDS	215..1645
Translation	MAGRGGSALLALCGALAACGWLLGAEAQEPGAPAAGMRRRRRLQQEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNMKKIVDQNTKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGSAHEYSSSPDDAIFQSLARAYSSFNPAMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKTYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVEGIDHDVTSAKDGDYWRLLIPGNYKLTASAPGYLAITKKVAVPYSPAAGVDFELESFSERKEEEKEELMEWWKMMSETLNF

Target ORF information:

RefSeq Version	NM_001873.4
Organism	Homo sapiens(human)
Definition	Homo sapiens carboxypeptidase E (CPE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001873.4

ORF Insert Sequence:

ATGGCCGGGC GAGGGGGCAG CGCGCTGCTG GCTCTGTGCG GGGCACTGGC TGCCTGCGGG 
TGGCTCCTGG GCGCCGAAGC CCAGGAGCCC GGGGCGCCCG CGGCGGGCAT GAGGCGGCGC 
CGGCGGCTGC AGCAAGAGGA CGGCATCTCC TTCGAGTACC ACCGCTACCC CGAGCTGCGC 
GAGGCGCTCG TGTCCGTGTG GCTGCAGTGC ACCGCCATCA GCAGGATTTA CACGGTGGGG 
CGCAGCTTCG AGGGCCGGGA GCTCCTGGTC ATCGAGCTGT CCGACAACCC TGGCGTCCAT 
GAGCCTGGTG AGCCTGAATT TAAATACATT GGGAATATGC ATGGGAATGA GGCTGTTGGA 
CGAGAACTGC TCATTTTCTT GGCCCAGTAC CTATGCAACG AATACCAGAA GGGGAACGAG 
ACAATTGTCA ACCTGATCCA CAGTACCCGC ATTCACATCA TGCCTTCCCT GAACCCAGAT 
GGCTTTGAGA AGGCAGCGTC TCAGCCTGGT GAACTCAAGG ACTGGTTTGT GGGTCGAAGC 
AATGCCCAGG GAATAGATCT GAACCGGAAC TTTCCAGACC TGGATAGGAT AGTGTACGTG 
AATGAGAAAG AAGGTGGTCC AAATAATCAT CTGTTGAAAA ATATGAAGAA AATTGTGGAT 
CAAAACACAA AGCTTGCTCC TGAGACCAAG GCTGTCATTC ATTGGATTAT GGATATTCCT 
TTTGTGCTTT CTGCCAATCT CCATGGAGGA GACCTTGTGG CCAATTATCC ATATGATGAG 
ACGCGGAGTG GTAGTGCTCA CGAATACAGC TCCTCCCCAG ATGACGCCAT TTTCCAAAGC 
TTGGCCCGGG CATACTCTTC TTTCAACCCG GCCATGTCTG ACCCCAATCG GCCACCATGT 
CGCAAGAATG ATGATGACAG CAGCTTTGTA GATGGAACCA CCAACGGTGG TGCTTGGTAC 
AGCGTACCTG GAGGGATGCA AGACTTCAAT TACCTTAGCA GCAACTGTTT TGAGATCACC 
GTGGAGCTTA GCTGTGAGAA GTTCCCACCT GAAGAGACTC TGAAGACCTA CTGGGAGGAT 
AACAAAAACT CCCTCATTAG CTACCTTGAG CAGATACACC GAGGAGTTAA AGGATTTGTC 
CGAGACCTTC AAGGTAACCC AATTGCGAAT GCCACCATCT CCGTGGAAGG AATAGACCAC 
GATGTTACAT CCGCAAAGGA TGGTGATTAC TGGAGATTGC TTATACCTGG AAACTATAAA 
CTTACAGCCT CAGCTCCAGG CTATCTGGCA ATAACAAAGA AAGTGGCAGT TCCTTACAGC 
CCTGCTGCTG GGGTTGATTT TGAACTGGAG TCATTTTCTG AAAGGAAAGA AGAGGAGAAG 
GAAGAATTGA TGGAATGGTG GAAAATGATG TCAGAAACTT TAAATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CPE ( NM_001873.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001864.1 Homo sapiens carboxypeptidase E (CPE), mRNA.

Price & Availability↑

CloneID	OHu09011
Clone ID Related Accession (Same CDS sequence)	NM_001873.2 , NM_001873.4 , NM_001873.3
Accession Version	NM_001873.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	carboxypeptidase E preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X51405.1, AA683379.1, AC012504.7 and AA883804.1. On Nov 24, 2015 this sequence version replaced NM_001873.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.83919.1, X51405.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGGC GAGGGGGCAG CGCGCTGCTG GCTCTGTGCG GGGCACTGGC TGCCTGCGGG 
TGGCTCCTGG GCGCCGAAGC CCAGGAGCCC GGGGCGCCCG CGGCGGGCAT GAGGCGGCGC 
CGGCGGCTGC AGCAAGAGGA CGGCATCTCC TTCGAGTACC ACCGCTACCC CGAGCTGCGC 
GAGGCGCTCG TGTCCGTGTG GCTGCAGTGC ACCGCCATCA GCAGGATTTA CACGGTGGGG 
CGCAGCTTCG AGGGCCGGGA GCTCCTGGTC ATCGAGCTGT CCGACAACCC TGGCGTCCAT 
GAGCCTGGTG AGCCTGAATT TAAATACATT GGGAATATGC ATGGGAATGA GGCTGTTGGA 
CGAGAACTGC TCATTTTCTT GGCCCAGTAC CTATGCAACG AATACCAGAA GGGGAACGAG 
ACAATTGTCA ACCTGATCCA CAGTACCCGC ATTCACATCA TGCCTTCCCT GAACCCAGAT 
GGCTTTGAGA AGGCAGCGTC TCAGCCTGGT GAACTCAAGG ACTGGTTTGT GGGTCGAAGC 
AATGCCCAGG GAATAGATCT GAACCGGAAC TTTCCAGACC TGGATAGGAT AGTGTACGTG 
AATGAGAAAG AAGGTGGTCC AAATAATCAT CTGTTGAAAA ATATGAAGAA AATTGTGGAT 
CAAAACACAA AGCTTGCTCC TGAGACCAAG GCTGTCATTC ATTGGATTAT GGATATTCCT 
TTTGTGCTTT CTGCCAATCT CCATGGAGGA GACCTTGTGG CCAATTATCC ATATGATGAG 
ACGCGGAGTG GTAGTGCTCA CGAATACAGC TCCTCCCCAG ATGACGCCAT TTTCCAAAGC 
TTGGCCCGGG CATACTCTTC TTTCAACCCG GCCATGTCTG ACCCCAATCG GCCACCATGT 
CGCAAGAATG ATGATGACAG CAGCTTTGTA GATGGAACCA CCAACGGTGG TGCTTGGTAC 
AGCGTACCTG GAGGGATGCA AGACTTCAAT TACCTTAGCA GCAACTGTTT TGAGATCACC 
GTGGAGCTTA GCTGTGAGAA GTTCCCACCT GAAGAGACTC TGAAGACCTA CTGGGAGGAT 
AACAAAAACT CCCTCATTAG CTACCTTGAG CAGATACACC GAGGAGTTAA AGGATTTGTC 
CGAGACCTTC AAGGTAACCC AATTGCGAAT GCCACCATCT CCGTGGAAGG AATAGACCAC 
GATGTTACAT CCGCAAAGGA TGGTGATTAC TGGAGATTGC TTATACCTGG AAACTATAAA 
CTTACAGCCT CAGCTCCAGG CTATCTGGCA ATAACAAAGA AAGTGGCAGT TCCTTACAGC 
CCTGCTGCTG GGGTTGATTT TGAACTGGAG TCATTTTCTG AAAGGAAAGA AGAGGAGAAG 
GAAGAATTGA TGGAATGGTG GAAAATGATG TCAGAAACTT TAAATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001864.1
CDS	281..1711
Translation	MAGRGGSALLALCGALAACGWLLGAEAQEPGAPAAGMRRRRRLQQEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNMKKIVDQNTKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGSAHEYSSSPDDAIFQSLARAYSSFNPAMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKTYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVEGIDHDVTSAKDGDYWRLLIPGNYKLTASAPGYLAITKKVAVPYSPAAGVDFELESFSERKEEEKEELMEWWKMMSETLNF

Target ORF information:

RefSeq Version	NM_001873.3
Organism	Homo sapiens(human)
Definition	Homo sapiens carboxypeptidase E (CPE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001873.3

ORF Insert Sequence:

ATGGCCGGGC GAGGGGGCAG CGCGCTGCTG GCTCTGTGCG GGGCACTGGC TGCCTGCGGG 
TGGCTCCTGG GCGCCGAAGC CCAGGAGCCC GGGGCGCCCG CGGCGGGCAT GAGGCGGCGC 
CGGCGGCTGC AGCAAGAGGA CGGCATCTCC TTCGAGTACC ACCGCTACCC CGAGCTGCGC 
GAGGCGCTCG TGTCCGTGTG GCTGCAGTGC ACCGCCATCA GCAGGATTTA CACGGTGGGG 
CGCAGCTTCG AGGGCCGGGA GCTCCTGGTC ATCGAGCTGT CCGACAACCC TGGCGTCCAT 
GAGCCTGGTG AGCCTGAATT TAAATACATT GGGAATATGC ATGGGAATGA GGCTGTTGGA 
CGAGAACTGC TCATTTTCTT GGCCCAGTAC CTATGCAACG AATACCAGAA GGGGAACGAG 
ACAATTGTCA ACCTGATCCA CAGTACCCGC ATTCACATCA TGCCTTCCCT GAACCCAGAT 
GGCTTTGAGA AGGCAGCGTC TCAGCCTGGT GAACTCAAGG ACTGGTTTGT GGGTCGAAGC 
AATGCCCAGG GAATAGATCT GAACCGGAAC TTTCCAGACC TGGATAGGAT AGTGTACGTG 
AATGAGAAAG AAGGTGGTCC AAATAATCAT CTGTTGAAAA ATATGAAGAA AATTGTGGAT 
CAAAACACAA AGCTTGCTCC TGAGACCAAG GCTGTCATTC ATTGGATTAT GGATATTCCT 
TTTGTGCTTT CTGCCAATCT CCATGGAGGA GACCTTGTGG CCAATTATCC ATATGATGAG 
ACGCGGAGTG GTAGTGCTCA CGAATACAGC TCCTCCCCAG ATGACGCCAT TTTCCAAAGC 
TTGGCCCGGG CATACTCTTC TTTCAACCCG GCCATGTCTG ACCCCAATCG GCCACCATGT 
CGCAAGAATG ATGATGACAG CAGCTTTGTA GATGGAACCA CCAACGGTGG TGCTTGGTAC 
AGCGTACCTG GAGGGATGCA AGACTTCAAT TACCTTAGCA GCAACTGTTT TGAGATCACC 
GTGGAGCTTA GCTGTGAGAA GTTCCCACCT GAAGAGACTC TGAAGACCTA CTGGGAGGAT 
AACAAAAACT CCCTCATTAG CTACCTTGAG CAGATACACC GAGGAGTTAA AGGATTTGTC 
CGAGACCTTC AAGGTAACCC AATTGCGAAT GCCACCATCT CCGTGGAAGG AATAGACCAC 
GATGTTACAT CCGCAAAGGA TGGTGATTAC TGGAGATTGC TTATACCTGG AAACTATAAA 
CTTACAGCCT CAGCTCCAGG CTATCTGGCA ATAACAAAGA AAGTGGCAGT TCCTTACAGC 
CCTGCTGCTG GGGTTGATTT TGAACTGGAG TCATTTTCTG AAAGGAAAGA AGAGGAGAAG 
GAAGAATTGA TGGAATGGTG GAAAATGATG TCAGAAACTT TAAATTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Carboxypeptidase E transmits its anti-migratory function in glioma cells via transcriptional regulation of cell architecture and motility regulating factors.
International journal of oncology51(2)702-714(2017 Aug)
Armento A,Ilina EI,Kaoma T,Muller A,Vallar L,Niclou SP,Kr?ger MA,Mittelbronn M,Naumann U

A Novel Single Nucleotide T980C Polymorphism in the Human Carboxypeptidase E Gene Results in Loss of Neuroprotective Function.
PloS one12(1)e0170169(2017)
Cong L,Cheng Y,Cawley NX,Murthy SR,Loh YP

Carboxypeptidase E (CPE) inhibits the secretion and activity of Wnt3a.
Oncogene35(50)6416-6428(2016 Dec)
Skalka N,Caspi M,Lahav-Ariel L,Loh YP,Hirschberg K,Rosin-Arbesfeld R

A human carboxypeptidase E/NF-?1 gene mutation in an Alzheimer's disease patient leads to dementia and depression in mice.
Translational psychiatry6(12)e973(2016 12)
Cheng Y,Cawley NX,Yanik T,Murthy SR,Liu C,Kasikci F,Abebe D,Loh YP

Carboxypeptidase E is a prediction marker for tumor recurrence in early-stage hepatocellular carcinoma.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(7)9745-53(2016 Jul)
Huang SF,Wu HD,Chen YT,Murthy SR,Chiu YT,Chang Y,Chang IC,Yang X,Loh YP

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CPE cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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