ADORA2A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADORA2A
Entrez Gene ID	135
Full Name	adenosine A2a receptor
Synonyms	A2aR,ADORA2,RDC8
General protein information	Preferred Names adenosine A2a receptor Names adenosine receptor A2a adenosine receptor subtype A2a
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q11.23
Summary	This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013].
Disorder MIM:	102776

mRNA and Protein(s)

mRNA	Protein	Name
NM_001278497.1	NP_001265426.1	adenosine receptor A2a
NM_001278498.1	NP_001265427.1	adenosine receptor A2a
NM_000675.5	NP_000666.2	adenosine receptor A2a
NM_001278499.1	NP_001265428.1	adenosine receptor A2a
NM_000675.6	NP_000666.2	adenosine receptor A2a
NM_001278499.2	NP_001265428.1	adenosine receptor A2a
NM_001278500.1	NP_001265429.1	adenosine receptor A2a

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	adora2ab	NP_001035125.1
Homo sapiens (human)	ADORA2A	NP_000666.2
Rattus norvegicus (Norway rat)	Adora2a	NP_445746.3
Xenopus tropicalis (tropical clawed frog)	adora2a	NP_001106398.1
Canis lupus familiaris (dog)	ADORA2A	NP_001003278.1
Bos taurus (cattle)	ADORA2A	XP_005218339.1
Mus musculus (house mouse)	Adora2a	NP_033760.2
Pan troglodytes (chimpanzee)	ADORA2A	XP_003953896.1
	ADORA2A	XP_001095636.1
Gallus gallus (chicken)	ADORA2A	XP_425280.2
Danio rerio (zebrafish)	adora2aa	NP_001034904.1

Gene Ontology
Bibliography

Related articles in PubMed

Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A
Cui C, Lin H, Shi Y, Pan R
Biochemical and biophysical research communications491(2)277-284(2017 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A2a receptor through NDRG3-Raf-ERK pathway.
Title: Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A

results unveil intracellular signaling pathways targeted by the A2AR, some of which might be key in modulating neutrophil functions.
Title: Early tyrosine phosphorylation events following adenosine A

Hypoxic postconditioning protects against apoptosis induced by reoxygenation via activation of adenosine A2a receptors on dermal microvascular endothelial cells.
Title: Hypoxic postconditioning attenuates apoptosis via activation of adenosine A

A1R signaling enhances A2AR-mediated neurodegeneration [review]
Title: Adenosine A1 and A2A Receptors in the Brain: Current Research and Their Role in Neurodegeneration.

However, the contribution of the A2AR to the control of impulsive reward seeking remains unknown. Using mice that were exposed to differential reward of low rate (DRL) schedules during Pavlovian-conditioning, second-order schedule discrimination, and the 5-choice serial reaction time task (5-CSRTT), we demonstrate that deficits of A2AR function promote impulsive responses.
Title: Adenosine A

The following ADORA2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADORA2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20931	NM_001278499.2 Latest version!	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu20931	NM_001278498.1 Latest version!	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu20931	NM_001278500.1 Latest version!	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu20931	NM_001278497.1 Latest version!	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu20931	NM_000675.6 Latest version!	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu20931	NM_000675.5	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu20931	NM_001278499.1	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADORA2A ( NM_001278497.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265426.1 Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20931
Clone ID Related Accession (Same CDS sequence)	NM_001278497.1 , NM_001278498.1 , NM_000675.5 , NM_001278499.1 , NM_000675.6 , NM_001278499.2 , NM_001278500.1
Accession Version	NM_001278497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	adenosine receptor A2a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK289871.1, CN408252.1, BG686029.1, HY249059.1 and CA307692.1. Transcript Variant: This variant (1) represents the longest transcript. Variants, 1, 2, 3, 4, and 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK289871.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465418 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265426.1
CDS	631..1869
Translation	MPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQGCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLPGERARSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLSHTNSVVNPFIYAYRIREFRQTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS

Target ORF information:

RefSeq Version	NM_001278497.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278497.1

ORF Insert Sequence:

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADORA2A ( NM_001278498.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265427.1 Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20931
Clone ID Related Accession (Same CDS sequence)	NM_001278497.1 , NM_001278498.1 , NM_000675.5 , NM_001278499.1 , NM_000675.6 , NM_001278499.2 , NM_001278500.1
Accession Version	NM_001278498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	adenosine receptor A2a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK289871.1, DA270141.1, CR456367.1, BG686029.1, HY249059.1 and CA307692.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants, 1, 2, 3, 4, and 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.276603.1, SRR1803613.49024.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265427.1
CDS	525..1763
Translation	MPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQGCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLPGERARSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLSHTNSVVNPFIYAYRIREFRQTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS

Target ORF information:

RefSeq Version	NM_001278498.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278498.1

ORF Insert Sequence:

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADORA2A ( NM_000675.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000666.2 Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu20931
Clone ID Related Accession (Same CDS sequence)	NM_001278497.1 , NM_001278498.1 , NM_000675.5 , NM_001278499.1 , NM_000675.6 , NM_001278499.2 , NM_001278500.1
Accession Version	NM_000675.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-08
Organism	Homo sapiens(human)
Product	adenosine receptor A2a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA942067.1, BC013780.1, AK123058.1 and CA307692.1. This sequence is a reference standard in the RefSeqGene project. On Jun 13, 2013 this sequence version replaced NM_000675.4. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants, 1, 2, 3, 4, and 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013780.1, SRR1163658.166108.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000666.2
CDS	460..1698
Translation	MPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQGCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLPGERARSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLSHTNSVVNPFIYAYRIREFRQTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS

Target ORF information:

RefSeq Version	NM_000675.5
Organism	Homo sapiens(human)
Definition	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000675.5

ORF Insert Sequence:

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADORA2A ( NM_001278499.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265428.1 Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu20931
Clone ID Related Accession (Same CDS sequence)	NM_001278497.1 , NM_001278498.1 , NM_000675.5 , NM_001278499.1 , NM_000675.6 , NM_001278499.2 , NM_001278500.1
Accession Version	NM_001278499.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	adenosine receptor A2a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA942067.1, CR456367.1, BG686029.1, HY249059.1 and CA307692.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants, 1, 2, 3, 4, and 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.377220.1, SRR1163655.300409.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265428.1
CDS	440..1678
Translation	MPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQGCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLPGERARSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLSHTNSVVNPFIYAYRIREFRQTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS

Target ORF information:

RefSeq Version	NM_001278499.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278499.1

ORF Insert Sequence:

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADORA2A ( NM_000675.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_000666.2 Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 3, mRNA.

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CloneID	OHu20931
Clone ID Related Accession (Same CDS sequence)	NM_001278497.1 , NM_001278498.1 , NM_000675.5 , NM_001278499.1 , NM_000675.6 , NM_001278499.2 , NM_001278500.1
Accession Version	NM_000675.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	adenosine receptor A2a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA942067.1, BC013780.1, AK123058.1 and CA307692.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000675.5. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants, 1, 2, 3, 4, and 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013780.1, SRR1163658.166108.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000337539.12/ ENSP00000336630.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000666.2
CDS	423..1661
Translation	MPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQGCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLPGERARSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLSHTNSVVNPFIYAYRIREFRQTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS

Target ORF information:

RefSeq Version	NM_000675.6
Organism	Homo sapiens(human)
Definition	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000675.6

ORF Insert Sequence:

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADORA2A ( NM_001278499.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265428.1 Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu20931
Clone ID Related Accession (Same CDS sequence)	NM_001278497.1 , NM_001278498.1 , NM_000675.5 , NM_001278499.1 , NM_000675.6 , NM_001278499.2 , NM_001278500.1
Accession Version	NM_001278499.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	adenosine receptor A2a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA942067.1, CR456367.1, BG686029.1, HY249059.1 and CA307692.1. On May 31, 2019 this sequence version replaced NM_001278499.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants, 1, 2, 3, 4, and 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.300409.1, SRR1163657.377220.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265428.1
CDS	403..1641
Translation	MPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQGCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLPGERARSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLSHTNSVVNPFIYAYRIREFRQTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS

Target ORF information:

RefSeq Version	NM_001278499.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278499.2

ORF Insert Sequence:

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADORA2A ( NM_001278500.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265429.1 Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu20931
Clone ID Related Accession (Same CDS sequence)	NM_001278497.1 , NM_001278498.1 , NM_000675.5 , NM_001278499.1 , NM_000675.6 , NM_001278499.2 , NM_001278500.1
Accession Version	NM_001278500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	adenosine receptor A2a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA781734.1, CR456367.1, BG686029.1, HY249059.1 and CA307692.1. Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. Variants, 1, 2, 3, 4, and 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.165374.1, SRR1803615.32292.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820699, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265429.1
CDS	307..1545
Translation	MPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQGCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLPGERARSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLSHTNSVVNPFIYAYRIREFRQTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS

Target ORF information:

RefSeq Version	NM_001278500.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278500.1

ORF Insert Sequence:

ATGCCCATCA TGGGCTCCTC GGTGTACATC ACGGTGGAGC TGGCCATTGC TGTGCTGGCC 
ATCCTGGGCA ATGTGCTGGT GTGCTGGGCC GTGTGGCTCA ACAGCAACCT GCAGAACGTC 
ACCAACTACT TTGTGGTGTC ACTGGCGGCG GCCGACATCG CAGTGGGTGT GCTCGCCATC 
CCCTTTGCCA TCACCATCAG CACCGGGTTC TGCGCTGCCT GCCACGGCTG CCTCTTCATT 
GCCTGCTTCG TCCTGGTCCT CACGCAGAGC TCCATCTTCA GTCTCCTGGC CATCGCCATT 
GACCGCTACA TTGCCATCCG CATCCCGCTC CGGTACAATG GCTTGGTGAC CGGCACGAGG 
GCTAAGGGCA TCATTGCCAT CTGCTGGGTG CTGTCGTTTG CCATCGGCCT GACTCCCATG 
CTAGGTTGGA ACAACTGCGG TCAGCCAAAG GAGGGCAAGA ACCACTCCCA GGGCTGCGGG 
GAGGGCCAAG TGGCCTGTCT CTTTGAGGAT GTGGTCCCCA TGAACTACAT GGTGTACTTC 
AACTTCTTTG CCTGTGTGCT GGTGCCCCTG CTGCTCATGC TGGGTGTCTA TTTGCGGATC 
TTCCTGGCGG CGCGACGACA GCTGAAGCAG ATGGAGAGCC AGCCTCTGCC GGGGGAGCGG 
GCACGGTCCA CACTGCAGAA GGAGGTCCAT GCTGCCAAGT CACTGGCCAT CATTGTGGGG 
CTCTTTGCCC TCTGCTGGCT GCCCCTACAC ATCATCAACT GCTTCACTTT CTTCTGCCCC 
GACTGCAGCC ACGCCCCTCT CTGGCTCATG TACCTGGCCA TCGTCCTCTC CCACACCAAT 
TCGGTTGTGA ATCCCTTCAT CTACGCCTAC CGTATCCGCG AGTTCCGCCA GACCTTCCGC 
AAGATCATTC GCAGCCACGT CCTGAGGCAG CAAGAACCTT TCAAGGCAGC TGGCACCAGT 
GCCCGGGTCT TGGCAGCTCA TGGCAGTGAC GGAGAGCAGG TCAGCCTCCG TCTCAACGGC 
CACCCGCCAG GAGTGTGGGC CAACGGCAGT GCTCCCCACC CTGAGCGGAG GCCCAATGGC 
TATGCCCTGG GGCTGGTGAG TGGAGGGAGT GCCCAAGAGT CCCAGGGGAA CACGGGCCTC 
CCAGACGTGG AGCTCCTTAG CCATGAGCTC AAGGGAGTGT GCCCAGAGCC CCCTGGCCTA 
GATGACCCCC TGGCCCAGGA TGGAGCAGGA GTGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A
Biochemical and biophysical research communications491(2)277-284(2017 09)
Cui C,Lin H,Shi Y,Pan R

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ADORA2A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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