OTOP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	OTOP1
Entrez Gene ID	133060
Full Name	otopetrin 1
General protein information	Preferred Names otopetrin 1 Names otopetrin-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p16.3
Summary	This gene encodes a transmembrane protein which belongs to the otopetrin domain protein family and is required for the formation of otoconia and otoliths, calcium carbonate biominerals within the inner ear of mammals that are required for the detection of linear acceleration and gravity. This gene modulates purinergic control of intracellular calcium in vestibular supporting cells. Naturally occurring mutations in the orthologous mouse gene are associated with nonsyndromic otoconia agenesis and a consequent balance defect. The orthologous mouse gene is also induced in white adipose tissue during obesity. The encoded protein is a component of a counterinflammatory pathway that attenuates obesity-induced adipose tissue inflammation and plays an adaptive role in maintaining metabolic homeostasis in obesity. [provided by RefSeq, Jul 2017].
Disorder MIM:	607806

mRNA and Protein(s)

mRNA	Protein	Name
NM_177998.3	NP_819056.1	proton channel OTOP1
NM_177998.1	NP_819056.1	otopetrin-1
NM_177998.2	NP_819056.1	proton channel OTOP1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	OTOP1	XP_545943.3
Mus musculus (house mouse)	Otop1	NP_766297.2
Rattus norvegicus (Norway rat)	Otop1	NP_852098.1
Gallus gallus (chicken)	OTOP1	XP_420790.3
Pan troglodytes (chimpanzee)	OTOP1	XP_526509.2
Bos taurus (cattle)	OTOP1	NP_001193713.1
Danio rerio (zebrafish)	otop1	NP_942098.1
Homo sapiens (human)	OTOP1	NP_819056.1
	OTOP1	XP_001097009.1
Xenopus tropicalis (tropical clawed frog)	otop1	XP_002939096.2

Gene Ontology
Bibliography

Related articles in PubMed

Otopetrin 1 protects mice from obesity-associated metabolic dysfunction through attenuating adipose tissue inflammation.
Wang GX, Cho KW, Uhm M, Hu CR, Li S, Cozacov Z, Xu AE, Cheng JX, Saltiel AR, Lumeng CN, Lin JD
Diabetes63(4)1340-52(2014 Apr)

Missense mutations in Otopetrin 1 affect subcellular localization and inhibition of purinergic signaling in vestibular supporting cells.
Kim E, Hyrc KL, Speck J, Salles FT, Lundberg YW, Goldberg MP, Kachar B, Warchol ME, Ornitz DM
Molecular and cellular neurosciences46(3)655-61(2011 Mar)

Identification of the Otopetrin Domain, a conserved domain in vertebrate otopetrins and invertebrate otopetrin-like family members.
Hughes I, Binkley J, Hurle B, Green ED, , Sidow A, Ornitz DM
BMC evolutionary biology841(2008 Feb)

Non-syndromic vestibular disorder with otoconial agenesis in tilted/mergulhador mice caused by mutations in otopetrin 1.
Hurle B, Ignatova E, Massironi SM, Mashimo T, Rios X, Thalmann I, Thalmann R, Ornitz DM
Human molecular genetics12(7)777-89(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following OTOP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OTOP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30028	NM_177998.3 Latest version!	Homo sapiens otopetrin 1 (OTOP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu30028	NM_177998.1	Homo sapiens otopetrin 1 (OTOP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu30028	NM_177998.2	Homo sapiens otopetrin 1 (OTOP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OTOP1 ( NM_177998.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_819056.1 Homo sapiens otopetrin 1 (OTOP1), mRNA.

Price & Availability↑

CloneID	OHu30028
Clone ID Related Accession (Same CDS sequence)	NM_177998.3 , NM_177998.1 , NM_177998.2
Accession Version	NM_177998.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	proton channel OTOP1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BK000653.1 and BC130432.1. On Jul 12, 2017 this sequence version replaced NM_177998.1. ##Evidence-Data-START## Transcript exon combination :: BC130430.1, BK000653.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2145893 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGCTCGAGG GCCTGGGGTC GCCCGCCTCG CCCCGGGCAG CTGCAAGCGC CTCGGTCGCA 
GGGTCGTCGG GGCCAGCGGC CTGCTCGCCT CCCTCGTCCT CGGCCCCGAG GTCCCCGGAA 
TCCCCGGCCC CCCGGCGGGG CGGTGTGCGC GCCAGCGTCC CACAGAAACT GGCCGAGATG 
CTGAGCAGCC AGTATGGGCT GATCGTGTTC GTGGCGGGGC TGCTGCTGCT GCTGGCCTGG 
GCCGTGCACG CCGCGGGCGT GAGCAAGAGC GACCTGCTGT GCTTCCTGAC GGCGCTCATG 
CTGCTGCAGC TGCTGTGGAT GCTGTGGTAC GTGGGCCGCA GCTCCGCGCA CCGCCGCCTC 
TTCCGCCTCA AGGACACGCA CGCGGGTGCC GGCTGGCTGC GCGGTAGTAT CACATTGTTT 
GCAGTCATTA CCGTCATCCT GGGATGCCTT AAAATTGGAT ACTTCATTGG ATTTTCAGAA 
TGTTTATCAG CCACTGAAGG AGTTTTCCCA GTCACCCATT CAGTGCATAC TTTGTTGCAG 
GTATATTTTC TTTGGGGGCA TGCAAAGGAT ATTATCCAGT CTTTCAAAAC ACTGGAAAGG 
TTTGGAGTGA TCCACTCGGT GTTCACCAAC CTGCTTCTGT GGGCCAATGG CGTCCTCAAT 
GAGTCAAAGC ACCAACTCAA TGAGCACAAG GAACGGCTCA TCACTCTGGG TTTTGGGAAC 
ATAACAACAG TTTTAGATGA CCACACACCG CAGTGTAACT GCACGCCCCC AACTCTGTGC 
ACTGCCATCT CCCACGGGAT CTACTACCTC TACCCCTTCA ACATAGAGTA TCAGATCCTG 
GCCTCCACAA TGCTCTACGT CCTGTGGAAG AACATCGGGC GCAAAGTTGA CAGCCATCAG 
CACCAGAAGA TGCAGTTCAA GTCTGATGGG GTCATGGTGG GCGCAGTCCT GGGCCTGACC 
GTGCTGGCCG CCACCATTGC TGTGGTGGTG GTATACCTGA TTCATATTGG GCGCTCCAAG 
ACCAAGAGCG AGTCGGCACT CATCATGTTC TACCTGTATG CCATCACCCT GCTGATGCTT 
ATGGGGGCTG CGGGGCTGGC TGGAATCCGG ATTTACAGGA TAGACGAGAA GTCACTGGAT 
GAGTCCAAAA ATCCGGCCCG CAAACTGGAC TCGGACCTCT TGGTGGGCAC TGCCTCGGGC 
TCCTGGCTTA TCTCCTGGGG CTCAATCTTG GCCATCCTCT GTGCTGAGGG CCACCCCCGC 
TACACCTGGT ACAACCTGCC CTACTCCATC CTGGCGATCG TGGAGAAGTA CATCCAGAAC 
CTCTTCATCT TTGAATCCAT TCACCGAGAG CCTGAAAAAC TCTCTGAGGA CATCCAAACC 
CTTCGGGTGG TCACAGTCTG CAATGGCAAC ACCATGCCCC TTGCTTCTTC CTGCCCCAAG 
AGTGGAGGTG TGGCCAGAGA TGTGGCTCCC CAGGGCAAGG ACATGCCACC AGCAGCCAAT 
GGAAATGTGT GCATGAGAGA AAGCCATGAC AAGGAGGAGG AGAAGCAGGA GGAGAGCAGC 
TGGGGAGGGA GCCCAAGCCC AGTCCGCCTT CCCCGTTTCT TACAGGGCAA CGCCAAGAGA 
AAAGTCCTGA GGAATATTGC AGCCTTCTTG TTCCTCTGCA ATATTTCGCT TTGGATACCT 
CCCGCCTTTG GCTGTCGACC TGAGTATGAC AATGGATTGG AGGAGATTGT CTTTGGCTTT 
GAACCCTGGA TAATTGTGGT CAACCTGGCC ATGCCTTTTT CTATTTTCTA TCGAATGCAC 
GCAGCTGCCT CCCTCTTTGA GGTCTATTGT AAGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_819056.1
CDS	31..1869
Translation	MLEGLGSPASPRAAASASVAGSSGPAACSPPSSSAPRSPESPAPRRGGVRASVPQKLAEMLSSQYGLIVFVAGLLLLLAWAVHAAGVSKSDLLCFLTALMLLQLLWMLWYVGRSSAHRRLFRLKDTHAGAGWLRGSITLFAVITVILGCLKIGYFIGFSECLSATEGVFPVTHSVHTLLQVYFLWGHAKDIIQSFKTLERFGVIHSVFTNLLLWANGVLNESKHQLNEHKERLITLGFGNITTVLDDHTPQCNCTPPTLCTAISHGIYYLYPFNIEYQILASTMLYVLWKNIGRKVDSHQHQKMQFKSDGVMVGAVLGLTVLAATIAVVVVYLIHIGRSKTKSESALIMFYLYAITLLMLMGAAGLAGIRIYRIDEKSLDESKNPARKLDSDLLVGTASGSWLISWGSILAILCAEGHPRYTWYNLPYSILAIVEKYIQNLFIFESIHREPEKLSEDIQTLRVVTVCNGNTMPLASSCPKSGGVARDVAPQGKDMPPAANGNVCMRESHDKEEEKQEESSWGGSPSPVRLPRFLQGNAKRKVLRNIAAFLFLCNISLWIPPAFGCRPEYDNGLEEIVFGFEPWIIVVNLAMPFSIFYRMHAAASLFEVYCKI

Target ORF information:

RefSeq Version	NM_177998.2
Organism	Homo sapiens(human)
Definition	Homo sapiens otopetrin 1 (OTOP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177998.2

ORF Insert Sequence:

ATGCTCGAGG GCCTGGGGTC GCCCGCCTCG CCCCGGGCAG CTGCAAGCGC CTCGGTCGCA 
GGGTCGTCGG GGCCAGCGGC CTGCTCGCCT CCCTCGTCCT CGGCCCCGAG GTCCCCGGAA 
TCCCCGGCCC CCCGGCGGGG CGGTGTGCGC GCCAGCGTCC CACAGAAACT GGCCGAGATG 
CTGAGCAGCC AGTATGGGCT GATCGTGTTC GTGGCGGGGC TGCTGCTGCT GCTGGCCTGG 
GCCGTGCACG CCGCGGGCGT GAGCAAGAGC GACCTGCTGT GCTTCCTGAC GGCGCTCATG 
CTGCTGCAGC TGCTGTGGAT GCTGTGGTAC GTGGGCCGCA GCTCCGCGCA CCGCCGCCTC 
TTCCGCCTCA AGGACACGCA CGCGGGTGCC GGCTGGCTGC GCGGTAGTAT CACATTGTTT 
GCAGTCATTA CCGTCATCCT GGGATGCCTT AAAATTGGAT ACTTCATTGG ATTTTCAGAA 
TGTTTATCAG CCACTGAAGG AGTTTTCCCA GTCACCCATT CAGTGCATAC TTTGTTGCAG 
GTATATTTTC TTTGGGGGCA TGCAAAGGAT ATTATCCAGT CTTTCAAAAC ACTGGAAAGG 
TTTGGAGTGA TCCACTCGGT GTTCACCAAC CTGCTTCTGT GGGCCAATGG CGTCCTCAAT 
GAGTCAAAGC ACCAACTCAA TGAGCACAAG GAACGGCTCA TCACTCTGGG TTTTGGGAAC 
ATAACAACAG TTTTAGATGA CCACACACCG CAGTGTAACT GCACGCCCCC AACTCTGTGC 
ACTGCCATCT CCCACGGGAT CTACTACCTC TACCCCTTCA ACATAGAGTA TCAGATCCTG 
GCCTCCACAA TGCTCTACGT CCTGTGGAAG AACATCGGGC GCAAAGTTGA CAGCCATCAG 
CACCAGAAGA TGCAGTTCAA GTCTGATGGG GTCATGGTGG GCGCAGTCCT GGGCCTGACC 
GTGCTGGCCG CCACCATTGC TGTGGTGGTG GTATACCTGA TTCATATTGG GCGCTCCAAG 
ACCAAGAGCG AGTCGGCACT CATCATGTTC TACCTGTATG CCATCACCCT GCTGATGCTT 
ATGGGGGCTG CGGGGCTGGC TGGAATCCGG ATTTACAGGA TAGACGAGAA GTCACTGGAT 
GAGTCCAAAA ATCCGGCCCG CAAACTGGAC TCGGACCTCT TGGTGGGCAC TGCCTCGGGC 
TCCTGGCTTA TCTCCTGGGG CTCAATCTTG GCCATCCTCT GTGCTGAGGG CCACCCCCGC 
TACACCTGGT ACAACCTGCC CTACTCCATC CTGGCGATCG TGGAGAAGTA CATCCAGAAC 
CTCTTCATCT TTGAATCCAT TCACCGAGAG CCTGAAAAAC TCTCTGAGGA CATCCAAACC 
CTTCGGGTGG TCACAGTCTG CAATGGCAAC ACCATGCCCC TTGCTTCTTC CTGCCCCAAG 
AGTGGAGGTG TGGCCAGAGA TGTGGCTCCC CAGGGCAAGG ACATGCCACC AGCAGCCAAT 
GGAAATGTGT GCATGAGAGA AAGCCATGAC AAGGAGGAGG AGAAGCAGGA GGAGAGCAGC 
TGGGGAGGGA GCCCAAGCCC AGTCCGCCTT CCCCGTTTCT TACAGGGCAA CGCCAAGAGA 
AAAGTCCTGA GGAATATTGC AGCCTTCTTG TTCCTCTGCA ATATTTCGCT TTGGATACCT 
CCCGCCTTTG GCTGTCGACC TGAGTATGAC AATGGATTGG AGGAGATTGT CTTTGGCTTT 
GAACCCTGGA TAATTGTGGT CAACCTGGCC ATGCCTTTTT CTATTTTCTA TCGAATGCAC 
GCAGCTGCCT CCCTCTTTGA GGTCTATTGT AAGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOP1 ( NM_177998.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_819056.1 Homo sapiens otopetrin 1 (OTOP1), mRNA.

Price & Availability↑

CloneID	OHu30028
Clone ID Related Accession (Same CDS sequence)	NM_177998.3 , NM_177998.1 , NM_177998.2
Accession Version	NM_177998.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	proton channel OTOP1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011744.8, BK000653.1 and BC130432.1. On Dec 30, 2019 this sequence version replaced NM_177998.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC130432.1, BK000653.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGCTCGAGG GCCTGGGGTC GCCCGCCTCG CCCCGGGCAG CTGCAAGCGC CTCGGTCGCA 
GGGTCGTCGG GGCCAGCGGC CTGCTCGCCT CCCTCGTCCT CGGCCCCGAG GTCCCCGGAA 
TCCCCGGCCC CCCGGCGGGG CGGTGTGCGC GCCAGCGTCC CACAGAAACT GGCCGAGATG 
CTGAGCAGCC AGTATGGGCT GATCGTGTTC GTGGCGGGGC TGCTGCTGCT GCTGGCCTGG 
GCCGTGCACG CCGCGGGCGT GAGCAAGAGC GACCTGCTGT GCTTCCTGAC GGCGCTCATG 
CTGCTGCAGC TGCTGTGGAT GCTGTGGTAC GTGGGCCGCA GCTCCGCGCA CCGCCGCCTC 
TTCCGCCTCA AGGACACGCA CGCGGGTGCC GGCTGGCTGC GCGGTAGTAT CACATTGTTT 
GCAGTCATTA CCGTCATCCT GGGATGCCTT AAAATTGGAT ACTTCATTGG ATTTTCAGAA 
TGTTTATCAG CCACTGAAGG AGTTTTCCCA GTCACCCATT CAGTGCATAC TTTGTTGCAG 
GTATATTTTC TTTGGGGGCA TGCAAAGGAT ATTATCCAGT CTTTCAAAAC ACTGGAAAGG 
TTTGGAGTGA TCCACTCGGT GTTCACCAAC CTGCTTCTGT GGGCCAATGG CGTCCTCAAT 
GAGTCAAAGC ACCAACTCAA TGAGCACAAG GAACGGCTCA TCACTCTGGG TTTTGGGAAC 
ATAACAACAG TTTTAGATGA CCACACACCG CAGTGTAACT GCACGCCCCC AACTCTGTGC 
ACTGCCATCT CCCACGGGAT CTACTACCTC TACCCCTTCA ACATAGAGTA TCAGATCCTG 
GCCTCCACAA TGCTCTACGT CCTGTGGAAG AACATCGGGC GCAAAGTTGA CAGCCATCAG 
CACCAGAAGA TGCAGTTCAA GTCTGATGGG GTCATGGTGG GCGCAGTCCT GGGCCTGACC 
GTGCTGGCCG CCACCATTGC TGTGGTGGTG GTATACCTGA TTCATATTGG GCGCTCCAAG 
ACCAAGAGCG AGTCGGCACT CATCATGTTC TACCTGTATG CCATCACCCT GCTGATGCTT 
ATGGGGGCTG CGGGGCTGGC TGGAATCCGG ATTTACAGGA TAGACGAGAA GTCACTGGAT 
GAGTCCAAAA ATCCGGCCCG CAAACTGGAC TCGGACCTCT TGGTGGGCAC TGCCTCGGGC 
TCCTGGCTTA TCTCCTGGGG CTCAATCTTG GCCATCCTCT GTGCTGAGGG CCACCCCCGC 
TACACCTGGT ACAACCTGCC CTACTCCATC CTGGCGATCG TGGAGAAGTA CATCCAGAAC 
CTCTTCATCT TTGAATCCAT TCACCGAGAG CCTGAAAAAC TCTCTGAGGA CATCCAAACC 
CTTCGGGTGG TCACAGTCTG CAATGGCAAC ACCATGCCCC TTGCTTCTTC CTGCCCCAAG 
AGTGGAGGTG TGGCCAGAGA TGTGGCTCCC CAGGGCAAGG ACATGCCACC AGCAGCCAAT 
GGAAATGTGT GCATGAGAGA AAGCCATGAC AAGGAGGAGG AGAAGCAGGA GGAGAGCAGC 
TGGGGAGGGA GCCCAAGCCC AGTCCGCCTT CCCCGTTTCT TACAGGGCAA CGCCAAGAGA 
AAAGTCCTGA GGAATATTGC AGCCTTCTTG TTCCTCTGCA ATATTTCGCT TTGGATACCT 
CCCGCCTTTG GCTGTCGACC TGAGTATGAC AATGGATTGG AGGAGATTGT CTTTGGCTTT 
GAACCCTGGA TAATTGTGGT CAACCTGGCC ATGCCTTTTT CTATTTTCTA TCGAATGCAC 
GCAGCTGCCT CCCTCTTTGA GGTCTATTGT AAGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_819056.1
CDS	66..1904
Translation	MLEGLGSPASPRAAASASVAGSSGPAACSPPSSSAPRSPESPAPRRGGVRASVPQKLAEMLSSQYGLIVFVAGLLLLLAWAVHAAGVSKSDLLCFLTALMLLQLLWMLWYVGRSSAHRRLFRLKDTHAGAGWLRGSITLFAVITVILGCLKIGYFIGFSECLSATEGVFPVTHSVHTLLQVYFLWGHAKDIIQSFKTLERFGVIHSVFTNLLLWANGVLNESKHQLNEHKERLITLGFGNITTVLDDHTPQCNCTPPTLCTAISHGIYYLYPFNIEYQILASTMLYVLWKNIGRKVDSHQHQKMQFKSDGVMVGAVLGLTVLAATIAVVVVYLIHIGRSKTKSESALIMFYLYAITLLMLMGAAGLAGIRIYRIDEKSLDESKNPARKLDSDLLVGTASGSWLISWGSILAILCAEGHPRYTWYNLPYSILAIVEKYIQNLFIFESIHREPEKLSEDIQTLRVVTVCNGNTMPLASSCPKSGGVARDVAPQGKDMPPAANGNVCMRESHDKEEEKQEESSWGGSPSPVRLPRFLQGNAKRKVLRNIAAFLFLCNISLWIPPAFGCRPEYDNGLEEIVFGFEPWIIVVNLAMPFSIFYRMHAAASLFEVYCKI

Target ORF information:

RefSeq Version	NM_177998.3
Organism	Homo sapiens(human)
Definition	Homo sapiens otopetrin 1 (OTOP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177998.3

ORF Insert Sequence:

ATGCTCGAGG GCCTGGGGTC GCCCGCCTCG CCCCGGGCAG CTGCAAGCGC CTCGGTCGCA 
GGGTCGTCGG GGCCAGCGGC CTGCTCGCCT CCCTCGTCCT CGGCCCCGAG GTCCCCGGAA 
TCCCCGGCCC CCCGGCGGGG CGGTGTGCGC GCCAGCGTCC CACAGAAACT GGCCGAGATG 
CTGAGCAGCC AGTATGGGCT GATCGTGTTC GTGGCGGGGC TGCTGCTGCT GCTGGCCTGG 
GCCGTGCACG CCGCGGGCGT GAGCAAGAGC GACCTGCTGT GCTTCCTGAC GGCGCTCATG 
CTGCTGCAGC TGCTGTGGAT GCTGTGGTAC GTGGGCCGCA GCTCCGCGCA CCGCCGCCTC 
TTCCGCCTCA AGGACACGCA CGCGGGTGCC GGCTGGCTGC GCGGTAGTAT CACATTGTTT 
GCAGTCATTA CCGTCATCCT GGGATGCCTT AAAATTGGAT ACTTCATTGG ATTTTCAGAA 
TGTTTATCAG CCACTGAAGG AGTTTTCCCA GTCACCCATT CAGTGCATAC TTTGTTGCAG 
GTATATTTTC TTTGGGGGCA TGCAAAGGAT ATTATCCAGT CTTTCAAAAC ACTGGAAAGG 
TTTGGAGTGA TCCACTCGGT GTTCACCAAC CTGCTTCTGT GGGCCAATGG CGTCCTCAAT 
GAGTCAAAGC ACCAACTCAA TGAGCACAAG GAACGGCTCA TCACTCTGGG TTTTGGGAAC 
ATAACAACAG TTTTAGATGA CCACACACCG CAGTGTAACT GCACGCCCCC AACTCTGTGC 
ACTGCCATCT CCCACGGGAT CTACTACCTC TACCCCTTCA ACATAGAGTA TCAGATCCTG 
GCCTCCACAA TGCTCTACGT CCTGTGGAAG AACATCGGGC GCAAAGTTGA CAGCCATCAG 
CACCAGAAGA TGCAGTTCAA GTCTGATGGG GTCATGGTGG GCGCAGTCCT GGGCCTGACC 
GTGCTGGCCG CCACCATTGC TGTGGTGGTG GTATACCTGA TTCATATTGG GCGCTCCAAG 
ACCAAGAGCG AGTCGGCACT CATCATGTTC TACCTGTATG CCATCACCCT GCTGATGCTT 
ATGGGGGCTG CGGGGCTGGC TGGAATCCGG ATTTACAGGA TAGACGAGAA GTCACTGGAT 
GAGTCCAAAA ATCCGGCCCG CAAACTGGAC TCGGACCTCT TGGTGGGCAC TGCCTCGGGC 
TCCTGGCTTA TCTCCTGGGG CTCAATCTTG GCCATCCTCT GTGCTGAGGG CCACCCCCGC 
TACACCTGGT ACAACCTGCC CTACTCCATC CTGGCGATCG TGGAGAAGTA CATCCAGAAC 
CTCTTCATCT TTGAATCCAT TCACCGAGAG CCTGAAAAAC TCTCTGAGGA CATCCAAACC 
CTTCGGGTGG TCACAGTCTG CAATGGCAAC ACCATGCCCC TTGCTTCTTC CTGCCCCAAG 
AGTGGAGGTG TGGCCAGAGA TGTGGCTCCC CAGGGCAAGG ACATGCCACC AGCAGCCAAT 
GGAAATGTGT GCATGAGAGA AAGCCATGAC AAGGAGGAGG AGAAGCAGGA GGAGAGCAGC 
TGGGGAGGGA GCCCAAGCCC AGTCCGCCTT CCCCGTTTCT TACAGGGCAA CGCCAAGAGA 
AAAGTCCTGA GGAATATTGC AGCCTTCTTG TTCCTCTGCA ATATTTCGCT TTGGATACCT 
CCCGCCTTTG GCTGTCGACC TGAGTATGAC AATGGATTGG AGGAGATTGT CTTTGGCTTT 
GAACCCTGGA TAATTGTGGT CAACCTGGCC ATGCCTTTTT CTATTTTCTA TCGAATGCAC 
GCAGCTGCCT CCCTCTTTGA GGTCTATTGT AAGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOP1 ( NM_177998.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_819056.1 Homo sapiens otopetrin 1 (OTOP1), mRNA.

Price & Availability↑

CloneID	OHu30028
Clone ID Related Accession (Same CDS sequence)	NM_177998.3 , NM_177998.1 , NM_177998.2
Accession Version	NM_177998.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Homo sapiens(Human)
Product	otopetrin-1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BK000653.1. ##Evidence-Data-START## Transcript exon combination :: BK000653.1, BC130430.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2145893 [ECO:0000350] ##Evidence-Data-END##

ATGCTCGAGG GCCTGGGGTC GCCCGCCTCG CCCCGGGCAG CTGCAAGCGC CTCGGTCGCA 
GGGTCGTCGG GGCCAGCGGC CTGCTCGCCT CCCTCGTCCT CGGCCCCGAG GTCCCCGGAA 
TCCCCGGCCC CCCGGCGGGG CGGTGTGCGC GCCAGCGTCC CACAGAAACT GGCCGAGATG 
CTGAGCAGCC AGTATGGGCT GATCGTGTTC GTGGCGGGGC TGCTGCTGCT GCTGGCCTGG 
GCCGTGCACG CCGCGGGCGT GAGCAAGAGC GACCTGCTGT GCTTCCTGAC GGCGCTCATG 
CTGCTGCAGC TGCTGTGGAT GCTGTGGTAC GTGGGCCGCA GCTCCGCGCA CCGCCGCCTC 
TTCCGCCTCA AGGACACGCA CGCGGGTGCC GGCTGGCTGC GCGGTAGTAT CACATTGTTT 
GCAGTCATTA CCGTCATCCT GGGATGCCTT AAAATTGGAT ACTTCATTGG ATTTTCAGAA 
TGTTTATCAG CCACTGAAGG AGTTTTCCCA GTCACCCATT CAGTGCATAC TTTGTTGCAG 
GTATATTTTC TTTGGGGGCA TGCAAAGGAT ATTATCCAGT CTTTCAAAAC ACTGGAAAGG 
TTTGGAGTGA TCCACTCGGT GTTCACCAAC CTGCTTCTGT GGGCCAATGG CGTCCTCAAT 
GAGTCAAAGC ACCAACTCAA TGAGCACAAG GAACGGCTCA TCACTCTGGG TTTTGGGAAC 
ATAACAACAG TTTTAGATGA CCACACACCG CAGTGTAACT GCACGCCCCC AACTCTGTGC 
ACTGCCATCT CCCACGGGAT CTACTACCTC TACCCCTTCA ACATAGAGTA TCAGATCCTG 
GCCTCCACAA TGCTCTACGT CCTGTGGAAG AACATCGGGC GCAAAGTTGA CAGCCATCAG 
CACCAGAAGA TGCAGTTCAA GTCTGATGGG GTCATGGTGG GCGCAGTCCT GGGCCTGACC 
GTGCTGGCCG CCACCATTGC TGTGGTGGTG GTATACCTGA TTCATATTGG GCGCTCCAAG 
ACCAAGAGCG AGTCGGCACT CATCATGTTC TACCTGTATG CCATCACCCT GCTGATGCTT 
ATGGGGGCTG CGGGGCTGGC TGGAATCCGG ATTTACAGGA TAGACGAGAA GTCACTGGAT 
GAGTCCAAAA ATCCGGCCCG CAAACTGGAC TCGGACCTCT TGGTGGGCAC TGCCTCGGGC 
TCCTGGCTTA TCTCCTGGGG CTCAATCTTG GCCATCCTCT GTGCTGAGGG CCACCCCCGC 
TACACCTGGT ACAACCTGCC CTACTCCATC CTGGCGATCG TGGAGAAGTA CATCCAGAAC 
CTCTTCATCT TTGAATCCAT TCACCGAGAG CCTGAAAAAC TCTCTGAGGA CATCCAAACC 
CTTCGGGTGG TCACAGTCTG CAATGGCAAC ACCATGCCCC TTGCTTCTTC CTGCCCCAAG 
AGTGGAGGTG TGGCCAGAGA TGTGGCTCCC CAGGGCAAGG ACATGCCACC AGCAGCCAAT 
GGAAATGTGT GCATGAGAGA AAGCCATGAC AAGGAGGAGG AGAAGCAGGA GGAGAGCAGC 
TGGGGAGGGA GCCCAAGCCC AGTCCGCCTT CCCCGTTTCT TACAGGGCAA CGCCAAGAGA 
AAAGTCCTGA GGAATATTGC AGCCTTCTTG TTCCTCTGCA ATATTTCGCT TTGGATACCT 
CCCGCCTTTG GCTGTCGACC TGAGTATGAC AATGGATTGG AGGAGATTGT CTTTGGCTTT 
GAACCCTGGA TAATTGTGGT CAACCTGGCC ATGCCTTTTT CTATTTTCTA TCGAATGCAC 
GCAGCTGCCT CCCTCTTTGA GGTCTATTGT AAGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_819056.1
CDS	31..1869
Translation	MLEGLGSPASPRAAASASVAGSSGPAACSPPSSSAPRSPESPAPRRGGVRASVPQKLAEMLSSQYGLIVFVAGLLLLLAWAVHAAGVSKSDLLCFLTALMLLQLLWMLWYVGRSSAHRRLFRLKDTHAGAGWLRGSITLFAVITVILGCLKIGYFIGFSECLSATEGVFPVTHSVHTLLQVYFLWGHAKDIIQSFKTLERFGVIHSVFTNLLLWANGVLNESKHQLNEHKERLITLGFGNITTVLDDHTPQCNCTPPTLCTAISHGIYYLYPFNIEYQILASTMLYVLWKNIGRKVDSHQHQKMQFKSDGVMVGAVLGLTVLAATIAVVVVYLIHIGRSKTKSESALIMFYLYAITLLMLMGAAGLAGIRIYRIDEKSLDESKNPARKLDSDLLVGTASGSWLISWGSILAILCAEGHPRYTWYNLPYSILAIVEKYIQNLFIFESIHREPEKLSEDIQTLRVVTVCNGNTMPLASSCPKSGGVARDVAPQGKDMPPAANGNVCMRESHDKEEEKQEESSWGGSPSPVRLPRFLQGNAKRKVLRNIAAFLFLCNISLWIPPAFGCRPEYDNGLEEIVFGFEPWIIVVNLAMPFSIFYRMHAAASLFEVYCKI

Target ORF information:

RefSeq Version	NM_177998.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens otopetrin 1 (OTOP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177998.1

ORF Insert Sequence:

ATGCTCGAGG GCCTGGGGTC GCCCGCCTCG CCCCGGGCAG CTGCAAGCGC CTCGGTCGCA 
GGGTCGTCGG GGCCAGCGGC CTGCTCGCCT CCCTCGTCCT CGGCCCCGAG GTCCCCGGAA 
TCCCCGGCCC CCCGGCGGGG CGGTGTGCGC GCCAGCGTCC CACAGAAACT GGCCGAGATG 
CTGAGCAGCC AGTATGGGCT GATCGTGTTC GTGGCGGGGC TGCTGCTGCT GCTGGCCTGG 
GCCGTGCACG CCGCGGGCGT GAGCAAGAGC GACCTGCTGT GCTTCCTGAC GGCGCTCATG 
CTGCTGCAGC TGCTGTGGAT GCTGTGGTAC GTGGGCCGCA GCTCCGCGCA CCGCCGCCTC 
TTCCGCCTCA AGGACACGCA CGCGGGTGCC GGCTGGCTGC GCGGTAGTAT CACATTGTTT 
GCAGTCATTA CCGTCATCCT GGGATGCCTT AAAATTGGAT ACTTCATTGG ATTTTCAGAA 
TGTTTATCAG CCACTGAAGG AGTTTTCCCA GTCACCCATT CAGTGCATAC TTTGTTGCAG 
GTATATTTTC TTTGGGGGCA TGCAAAGGAT ATTATCCAGT CTTTCAAAAC ACTGGAAAGG 
TTTGGAGTGA TCCACTCGGT GTTCACCAAC CTGCTTCTGT GGGCCAATGG CGTCCTCAAT 
GAGTCAAAGC ACCAACTCAA TGAGCACAAG GAACGGCTCA TCACTCTGGG TTTTGGGAAC 
ATAACAACAG TTTTAGATGA CCACACACCG CAGTGTAACT GCACGCCCCC AACTCTGTGC 
ACTGCCATCT CCCACGGGAT CTACTACCTC TACCCCTTCA ACATAGAGTA TCAGATCCTG 
GCCTCCACAA TGCTCTACGT CCTGTGGAAG AACATCGGGC GCAAAGTTGA CAGCCATCAG 
CACCAGAAGA TGCAGTTCAA GTCTGATGGG GTCATGGTGG GCGCAGTCCT GGGCCTGACC 
GTGCTGGCCG CCACCATTGC TGTGGTGGTG GTATACCTGA TTCATATTGG GCGCTCCAAG 
ACCAAGAGCG AGTCGGCACT CATCATGTTC TACCTGTATG CCATCACCCT GCTGATGCTT 
ATGGGGGCTG CGGGGCTGGC TGGAATCCGG ATTTACAGGA TAGACGAGAA GTCACTGGAT 
GAGTCCAAAA ATCCGGCCCG CAAACTGGAC TCGGACCTCT TGGTGGGCAC TGCCTCGGGC 
TCCTGGCTTA TCTCCTGGGG CTCAATCTTG GCCATCCTCT GTGCTGAGGG CCACCCCCGC 
TACACCTGGT ACAACCTGCC CTACTCCATC CTGGCGATCG TGGAGAAGTA CATCCAGAAC 
CTCTTCATCT TTGAATCCAT TCACCGAGAG CCTGAAAAAC TCTCTGAGGA CATCCAAACC 
CTTCGGGTGG TCACAGTCTG CAATGGCAAC ACCATGCCCC TTGCTTCTTC CTGCCCCAAG 
AGTGGAGGTG TGGCCAGAGA TGTGGCTCCC CAGGGCAAGG ACATGCCACC AGCAGCCAAT 
GGAAATGTGT GCATGAGAGA AAGCCATGAC AAGGAGGAGG AGAAGCAGGA GGAGAGCAGC 
TGGGGAGGGA GCCCAAGCCC AGTCCGCCTT CCCCGTTTCT TACAGGGCAA CGCCAAGAGA 
AAAGTCCTGA GGAATATTGC AGCCTTCTTG TTCCTCTGCA ATATTTCGCT TTGGATACCT 
CCCGCCTTTG GCTGTCGACC TGAGTATGAC AATGGATTGG AGGAGATTGT CTTTGGCTTT 
GAACCCTGGA TAATTGTGGT CAACCTGGCC ATGCCTTTTT CTATTTTCTA TCGAATGCAC 
GCAGCTGCCT CCCTCTTTGA GGTCTATTGT AAGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Otopetrin 1 protects mice from obesity-associated metabolic dysfunction through attenuating adipose tissue inflammation.
Diabetes63(4)1340-52(2014 Apr)
Wang GX,Cho KW,Uhm M,Hu CR,Li S,Cozacov Z,Xu AE,Cheng JX,Saltiel AR,Lumeng CN,Lin JD

Missense mutations in Otopetrin 1 affect subcellular localization and inhibition of purinergic signaling in vestibular supporting cells.
Molecular and cellular neurosciences46(3)655-61(2011 Mar)
Kim E,Hyrc KL,Speck J,Salles FT,Lundberg YW,Goldberg MP,Kachar B,Warchol ME,Ornitz DM

Identification of the Otopetrin Domain, a conserved domain in vertebrate otopetrins and invertebrate otopetrin-like family members.
BMC evolutionary biology841(2008 Feb)
Hughes I,Binkley J,Hurle B,Green ED,,Sidow A,Ornitz DM

Non-syndromic vestibular disorder with otoconial agenesis in tilted/mergulhador mice caused by mutations in otopetrin 1.
Human molecular genetics12(7)777-89(2003 Apr)
Hurle B,Ignatova E,Massironi SM,Mashimo T,Rios X,Thalmann I,Thalmann R,Ornitz DM

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OTOP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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