COL17A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL17A1
Entrez Gene ID	1308
Full Name	collagen type XVII alpha 1 chain
Synonyms	BA16H23.2,BP180,BPA-2,BPAG2,ERED,LAD-1
General protein information	Preferred Names collagen type XVII alpha 1 chain Names collagen alpha-1(XVII) chain 180 kDa bullous pemphigoid antigen 2 alpha 1 type XVII collagen bA16H23.2 (collagen, type XVII, alpha 1 (BP180)) bullous pemphigoid antigen 2 (180kD) collagen XVII, alpha-1 polypeptide collagen, type XVII, alpha 1 type XVII collagen alpha-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q25.1
Summary	This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008].
Disorder MIM:	113811
Disorder Html:	Epidermolysis bullosa, junctional, non-Herlitz type, 226650 (3); Epidermolysis bullosa, junctional, localisata variant, 226650 (3); Epithelial recurrent erosion dystrophy, 122400 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000494.4	NP_000485.3	collagen alpha-1(XVII) chain
NM_000494.3	NP_000485.3	collagen alpha-1(XVII) chain

Pathways from BioSystems

Homologies

Homo sapiens (human)	COL17A1	NP_000485.3
Pan troglodytes (chimpanzee)	COL17A1	XP_003951824.1
Canis lupus familiaris (dog)	COL17A1	XP_535006.3
Bos taurus (cattle)	COL17A1	NP_001095450.1

Gene Ontology
Bibliography

Related articles in PubMed

C-Terminal Processing of Collagen XVII Induces Neoepitopes for Linear IgA Dermatosis Autoantibodies.
Toyonaga E, Nishie W, Izumi K, Natsuga K, Ujiie H, Iwata H, Yamagami J, Hirako Y, Sawamura D, Fujimoto W, Shimizu H
The Journal of investigative dermatology137(12)2552-2559(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

C-terminal processing induces dynamic structural changes and neoepitopes for linear IgA dermatosis autoantibodies on COL17
Title: C-Terminal Processing of Collagen XVII Induces Neoepitopes for Linear IgA Dermatosis Autoantibodies.

Case Report: IgA autoantibodies targeting the NC16A domain rather than the shed ectodomains of COL17 resulted in linear IgA bullous dermatosis in a pregnant woman.
Title: Linear IgA Bullous Dermatosis in a Pregnant Woman with Autoantibodies to the Non-collagenous 16A Domain of Type XVII Collagen.

As a consequence of altered maturation and decreased stability of collagen XVII trimers, reduced collagen XVII is incorporated into the cell membrane, resulting in compromised dermal-epidermal adhesion. Taken together, using this genetic model, we provide the first proof that alteration of the coiled-coil structure destabilizes oligomerization and impairs physiological shedding of collagen XVII in vivo.
Title: Amino acid duplication in the coiled-coil structure of collagen XVII alters its maturation and trimerization causing mild junctional epidermolysis bullosa.

Data suggest that type XVII collagen (COL17) internalization/macropinocytosis by keratinocytes induced by IgG autoantibodies from patients with bullous pemphigoid (BP) is mediated by PKC (protein kinase C) calcium signaling pathway; BP IgG induced overabundant phosphorylation of COL17.
Title: Macropinocytosis of type XVII collagen induced by bullous pemphigoid IgG is regulated via protein kinase C.

Results show that aberrant epigenetic control is a key driver of COL17A1 gene misexpression and tumor cell invasion in a variety of epithelial neoplasm. These findings have significant clinical implications, suggesting that the COL17A1 promoter methylation status can be used to predict patient outcome.
Title: In epithelial cancers, aberrant

The following COL17A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL17A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22348	NM_000494.4 Latest version!	Homo sapiens collagen type XVII alpha 1 chain (COL17A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
View Old Accession Versions >>
OHu22348	NM_000494.3	Homo sapiens collagen type XVII alpha 1 chain (COL17A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL17A1 ( NM_000494.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000485.3 Homo sapiens collagen type XVII alpha 1 chain (COL17A1), mRNA.

Price & Availability↑

CloneID	OHu22348
Clone ID Related Accession (Same CDS sequence)	NM_000494.4 , NM_000494.3
Accession Version	NM_000494.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	collagen alpha-1(XVII) chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA646288.1, M91669.1, AL138761.12, AF136185.1 and AI572644.1. This sequence is a reference standard in the RefSeqGene project. On Jul 12, 2018 this sequence version replaced NM_000494.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M91669.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000648076.2/ ENSP00000497653.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATGTAA CCAAGAAAAA CAAACGAGAT GGAACTGAAG TCACTGAGAG AATTGTCACT 
GAAACAGTAA CCACAAGACT TACATCCTTA CCACCAAAAG GCGGGACCAG CAATGGCTAT 
GCTAAAACAG CCTCTCTTGG TGGAGGGAGC CGGCTGGAGA AACAAAGCCT GACTCATGGC 
AGCAGCGGCT ACATAAACTC AACTGGAAGC ACACGAGGCC ATGCCTCCAC CTCTAGTTAC 
AGGAGGGCTC ACTCACCTGC CTCCACTCTG CCCAACTCCC CAGGCTCAAC CTTTGAAAGG 
AAAACTCACG TTACCCGCCA TGCGTATGAA GGGAGCTCCA GTGGCAACTC TTCTCCGGAG 
TACCCTCGGA AGGAATTTGC ATCTTCTTCA ACCAGAGGAC GGAGTCAAAC ACGAGAGAGT 
GAAATTCGAG TTCGACTGCA GAGTGCGTCC CCATCCACCC GATGGACAGA ATTGGATGAT 
GTTAAGCGTT TGCTCAAGGG GAGTCGATCG GCAAGTGTGA GCCCCACCCG GAATTCCTCC 
AACACACTCC CCATCCCCAA GAAAGGCACT GTGGAGACCA AAATTGTGAC AGCGAGCTCC 
CAGTCGGTGT CAGGCACCTA CGATGCAACG ATCCTGGATG CCAACCTTCC CTCCCATGTG 
TGGTCCTCCA CCCTGCCCGC GGGGTCCTCC ATGGGGACCT ATCACAACAA CATGACAACC 
CAGAGCTCAT CCCTCCTCAA CACCAATGCC TACTCTGCGG GATCAGTCTT CGGAGTTCCA 
AACAACATGG CGTCCTGCTC ACCCACTTTG CACCCTGGAC TCAGCACATC CTCCTCAGTG 
TTTGGCATGC AGAACAATCT GGCCCCCAGC TTGACCACCC TGTCCCATGG CACCACCACC 
ACTTCCACAG CATATGGGGT GAAGAAAAAC ATGCCCCAGA GTCCTGCGGC TGTGAACACT 
GGCGTTTCCA CCTCCGCCGC CTGCACCACA AGTGTGCAGA GCGATGACCT TTTGCACAAG 
GACTGCAAGT TCCTGATCCT AGAGAAAGAC AACACACCTG CCAAGAAGGA GATGGAGCTG 
CTCATCATGA CCAAGGACAG CGGGAAGGTC TTTACAGCCT CCCCTGCCAG CATCGCTGCA 
ACTTCTTTTT CAGAAGACAC CCTAAAAAAA GAAAAGCAAG CTGCCTACAA TGCTGACTCA 
GGCCTAAAAG CCGAAGCTAA TGGAGACCTG AAGACTGTGT CCACAAAGGG CAAGACCACC 
ACTGCAGATA TCCACAGCTA CGGCAGCAGT GGTGGTGGTG GCAGTGGAGG AGGTGGCGGT 
GTTGGTGGCG CTGGCGGCGG CCCTTGGGGA CCAGCGCCAG CCTGGTGCCC CTGCGGCTCC 
TGCTGCAGCT GGTGGAAGTG GCTGCTGGGC CTGCTGCTCA CCTGGCTGCT ACTCCTGGGG 
CTGCTCTTCG GCCTCATTGC TCTGGCGGAG GAGGTGAGGA AGCTGAAGGC GCGTGTGGAT 
GAGCTGGAGA GGATCAGGAG GAGCATACTG CCCTATGGGG ACAGCATGGA TAGAATAGAA 
AAGGACCGCC TCCAGGGCAT GGCACCCGCG GCGGGAGCAG ACCTGGACAA AATTGGGCTG 
CACAGTGACA GCCAGGAGGA GCTCTGGATG TTCGTGAGGA AGAAGCTAAT GATGGAACAG 
GAAAATGGAA ATCTCCGAGG AAGCCCTGGC CCTAAAGGTG ACATGGGAAG TCCAGGCCCT 
AAAGGAGATC GAGGGTTCCC TGGGACTCCA GGTATCCCTG GGCCCTTGGG CCACCCAGGT 
CCACAAGGAC CAAAGGGTCA AAAAGGCAGC GTGGGAGATC CTGGCATGGA AGGCCCCATG 
GGCCAGAGAG GGCGAGAAGG CCCCATGGGA CCTCGTGGTG AGGCAGGGCC TCCTGGATCT 
GGAGAGAAAG GGGAAAGAGG GGCTGCTGGT GAACCAGGTC CTCATGGCCC ACCTGGTGTC 
CCAGGTTCTG TGGGTCCCAA AGGTTCCAGC GGCTCTCCTG GCCCACAGGG CCCTCCAGGT 
CCTGTAGGTC TCCAAGGGCT CCGAGGTGAA GTAGGACTTC CTGGTGTCAA AGGTGACAAA 
GGACCAATGG GACCACCAGG ACCCAAAGGT GACCAGGGTG AGAAAGGACC TCGAGGCCTC 
ACAGGCGAGC CTGGCATGAG AGGTTTGCCT GGTGCTGTTG GTGAGCCCGG GGCTAAAGGA 
GCAATGGGTC CTGCTGGCCC AGACGGACAC CAAGGCCCAA GAGGTGAACA AGGTCTTACT 
GGGATGCCTG GAATCCGTGG CCCACCAGGA CCTTCTGGAG ACCCAGGAAA GCCAGGTCTC 
ACAGGACCCC AGGGACCTCA GGGACTTCCC GGTACCCCTG GCCGACCAGG AATAAAAGGT 
GAACCAGGAG CTCCAGGCAA GATCGTGACT TCGGAGGGGT CATCGATGCT CACTGTCCCA 
GGCCCCCCAG GACCTCCTGG AGCCATGGGA CCCCCAGGAC CTCCAGGTGC CCCAGGCCCT 
GCCGGCCCAG CTGGTCTCCC AGGACATCAA GAAGTTCTTA ATTTACAAGG TCCCCCAGGC 
CCACCCGGCC CACGCGGGCC ACCAGGGCCT TCCATTCCAG GCCCACCAGG ACCCCGAGGC 
CCACCAGGGG AGGGTTTGCC AGGCCCACCA GGCCCACCAG GATCGTTCCT GTCCAACTCA 
GAAACCTTCC TCTCCGGCCC CCCAGGCCCA CCTGGCCCCC CAGGTCCCAA GGGAGACCAA 
GGTCCCCCAG GCCCCAGAGG ACACCAAGGC GAGCAAGGCC TCCCAGGTTT CTCAACCTCA 
GGGTCCAGTT CTTTCGGACT CAACCTTCAG GGACCACCAG GCCCACCTGG CCCCCAGGGA 
CCCAAAGGTG ACAAAGGTGA TCCAGGTGTT CCAGGGGCTC TTGGCATTCC TAGTGGTCCT 
TCTGAAGGGG GATCATCAAG TACCATGTAC GTGTCAGGCC CGCCAGGGCC CCCTGGGCCC 
CCTGGGCCTC CGGGCTCTAT CAGCAGCTCT GGCCAGGAGA TTCAGCAGTA CATCTCTGAG 
TACATGCAGA GTGACAGTAT TAGATCTTAC CTATCCGGAG TTCAGGGTCC CCCAGGCCCA 
CCTGGTCCCC CAGGACCTGT CACCACCATC ACAGGCGAGA CTTTCGACTA CTCAGAGCTG 
GCAAGCCACG TTGTGAGCTA CTTACGGACT TCGGGGTACG GTGTCAGCTT GTTCTCGTCC 
TCCATCTCTT CTGAAGACAT TCTGGCTGTG CTGCAGCGGG ATGACGTGCG TCAGTACCTA 
CGTCAGTACT TGATGGGCCC TCGGGGTCCG CCAGGGCCAC CAGGAGCCAG TGGAGATGGG 
TCCCTCCTGT CTTTGGACTA TGCAGAGCTG AGTAGTCGCA TTCTCAGCTA CATGTCGAGT 
TCTGGGATCA GCATTGGGCT TCCTGGTCCC CCGGGGCCCC CTGGCTTGCC GGGAACCTCC 
TATGAGGAGC TCCTCTCCTT GCTGCGAGGG TCTGAATTCA GAGGCATCGT TGGACCCCCA 
GGTCCCCCGG GTCCACCAGG GATCCCAGGC AATGTGTGGT CCAGCATCAG CGTGGAGGAC 
CTCTCGTCTT ACTTACATAC TGCCGGCTTG TCATTCATCC CAGGCCCTCC AGGACCTCCT 
GGTCCCCCAG GGCCTCGAGG GCCCCCGGGT GTCTCAGGAG CCCTGGCAAC CTATGCAGCT 
GAAAACAGCG ACAGCTTCCG GAGCGAGCTG ATCAGCTACC TCACAAGTCC TGATGTGCGC 
AGCTTCATTG TTGGCCCCCC AGGCCCTCCT GGGCCGCAGG GACCCCCTGG GGACAGCCGC 
CTCCTGTCCA CGGATGCCTC CCACAGTCGG GGTAGCAGCT CCTCCTCACA CAGCTCATCT 
GTCAGGCGGG GCAGCTCCTA CAGCTCTTCC ATGAGCACAG GAGGAGGTGG TGCAGGCTCC 
CTGGGTGCAG GCGGTGCCTT TGGTGAAGCT GCAGGAGACA GGGGTCCCTA TGGCACTGAC 
ATCGGCCCAG GCGGAGGCTA TGGGGCAGCA GCAGAAGGCG GCATGTATGC TGGCAATGGC 
GGACTATTGG GAGCTGACTT TGCTGGAGAT CTGGATTACA ATGAGCTGGC TGTGAGGGTG 
TCAGAGAGCA TGCAGCGTCA GGGCCTACTG CAAGGGATGG CCTACACTGT CCAGGGCCCA 
CCAGGCCAGC CTGGGCCACA GGGGCCACCC GGCATCAGCA AGGTCTTCTC TGCCTACAGC 
AACGTGACTG CGGACCTCAT GGACTTCTTC CAAACTTATG GAGCCATTCA AGGACCCCCT 
GGGCAAAAAG GAGAGATGGG CACTCCAGGA CCCAAAGGTG ACAGGGGCCC TGCTGGGCCA 
CCAGGTCATC CTGGGCCACC TGGCCCTCGA GGACACAAGG GAGAAAAAGG AGACAAAGGT 
GACCAAGTCT ATGCTGGGCG GAGAAGGAGA AGAAGTATTG CTGTCAAGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000485.3
CDS	170..4663
Translation	MDVTKKNKRDGTEVTERIVTETVTTRLTSLPPKGGTSNGYAKTASLGGGSRLEKQSLTHGSSGYINSTGSTRGHASTSSYRRAHSPASTLPNSPGSTFERKTHVTRHAYEGSSSGNSSPEYPRKEFASSSTRGRSQTRESEIRVRLQSASPSTRWTELDDVKRLLKGSRSASVSPTRNSSNTLPIPKKGTVETKIVTASSQSVSGTYDATILDANLPSHVWSSTLPAGSSMGTYHNNMTTQSSSLLNTNAYSAGSVFGVPNNMASCSPTLHPGLSTSSSVFGMQNNLAPSLTTLSHGTTTTSTAYGVKKNMPQSPAAVNTGVSTSAACTTSVQSDDLLHKDCKFLILEKDNTPAKKEMELLIMTKDSGKVFTASPASIAATSFSEDTLKKEKQAAYNADSGLKAEANGDLKTVSTKGKTTTADIHSYGSSGGGGSGGGGGVGGAGGGPWGPAPAWCPCGSCCSWWKWLLGLLLTWLLLLGLLFGLIALAEEVRKLKARVDELERIRRSILPYGDSMDRIEKDRLQGMAPAAGADLDKIGLHSDSQEELWMFVRKKLMMEQENGNLRGSPGPKGDMGSPGPKGDRGFPGTPGIPGPLGHPGPQGPKGQKGSVGDPGMEGPMGQRGREGPMGPRGEAGPPGSGEKGERGAAGEPGPHGPPGVPGSVGPKGSSGSPGPQGPPGPVGLQGLRGEVGLPGVKGDKGPMGPPGPKGDQGEKGPRGLTGEPGMRGLPGAVGEPGAKGAMGPAGPDGHQGPRGEQGLTGMPGIRGPPGPSGDPGKPGLTGPQGPQGLPGTPGRPGIKGEPGAPGKIVTSEGSSMLTVPGPPGPPGAMGPPGPPGAPGPAGPAGLPGHQEVLNLQGPPGPPGPRGPPGPSIPGPPGPRGPPGEGLPGPPGPPGSFLSNSETFLSGPPGPPGPPGPKGDQGPPGPRGHQGEQGLPGFSTSGSSSFGLNLQGPPGPPGPQGPKGDKGDPGVPGALGIPSGPSEGGSSSTMYVSGPPGPPGPPGPPGSISSSGQEIQQYISEYMQSDSIRSYLSGVQGPPGPPGPPGPVTTITGETFDYSELASHVVSYLRTSGYGVSLFSSSISSEDILAVLQRDDVRQYLRQYLMGPRGPPGPPGASGDGSLLSLDYAELSSRILSYMSSSGISIGLPGPPGPPGLPGTSYEELLSLLRGSEFRGIVGPPGPPGPPGIPGNVWSSISVEDLSSYLHTAGLSFIPGPPGPPGPPGPRGPPGVSGALATYAAENSDSFRSELISYLTSPDVRSFIVGPPGPPGPQGPPGDSRLLSTDASHSRGSSSSSHSSSVRRGSSYSSSMSTGGGGAGSLGAGGAFGEAAGDRGPYGTDIGPGGGYGAAAEGGMYAGNGGLLGADFAGDLDYNELAVRVSESMQRQGLLQGMAYTVQGPPGQPGPQGPPGISKVFSAYSNVTADLMDFFQTYGAIQGPPGQKGEMGTPGPKGDRGPAGPPGHPGPPGPRGHKGEKGDKGDQVYAGRRRRRSIAVKP

Target ORF information:

RefSeq Version	NM_000494.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XVII alpha 1 chain (COL17A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000494.4

ORF Insert Sequence:

ATGGATGTAA CCAAGAAAAA CAAACGAGAT GGAACTGAAG TCACTGAGAG AATTGTCACT 
GAAACAGTAA CCACAAGACT TACATCCTTA CCACCAAAAG GCGGGACCAG CAATGGCTAT 
GCTAAAACAG CCTCTCTTGG TGGAGGGAGC CGGCTGGAGA AACAAAGCCT GACTCATGGC 
AGCAGCGGCT ACATAAACTC AACTGGAAGC ACACGAGGCC ATGCCTCCAC CTCTAGTTAC 
AGGAGGGCTC ACTCACCTGC CTCCACTCTG CCCAACTCCC CAGGCTCAAC CTTTGAAAGG 
AAAACTCACG TTACCCGCCA TGCGTATGAA GGGAGCTCCA GTGGCAACTC TTCTCCGGAG 
TACCCTCGGA AGGAATTTGC ATCTTCTTCA ACCAGAGGAC GGAGTCAAAC ACGAGAGAGT 
GAAATTCGAG TTCGACTGCA GAGTGCGTCC CCATCCACCC GATGGACAGA ATTGGATGAT 
GTTAAGCGTT TGCTCAAGGG GAGTCGATCG GCAAGTGTGA GCCCCACCCG GAATTCCTCC 
AACACACTCC CCATCCCCAA GAAAGGCACT GTGGAGACCA AAATTGTGAC AGCGAGCTCC 
CAGTCGGTGT CAGGCACCTA CGATGCAACG ATCCTGGATG CCAACCTTCC CTCCCATGTG 
TGGTCCTCCA CCCTGCCCGC GGGGTCCTCC ATGGGGACCT ATCACAACAA CATGACAACC 
CAGAGCTCAT CCCTCCTCAA CACCAATGCC TACTCTGCGG GATCAGTCTT CGGAGTTCCA 
AACAACATGG CGTCCTGCTC ACCCACTTTG CACCCTGGAC TCAGCACATC CTCCTCAGTG 
TTTGGCATGC AGAACAATCT GGCCCCCAGC TTGACCACCC TGTCCCATGG CACCACCACC 
ACTTCCACAG CATATGGGGT GAAGAAAAAC ATGCCCCAGA GTCCTGCGGC TGTGAACACT 
GGCGTTTCCA CCTCCGCCGC CTGCACCACA AGTGTGCAGA GCGATGACCT TTTGCACAAG 
GACTGCAAGT TCCTGATCCT AGAGAAAGAC AACACACCTG CCAAGAAGGA GATGGAGCTG 
CTCATCATGA CCAAGGACAG CGGGAAGGTC TTTACAGCCT CCCCTGCCAG CATCGCTGCA 
ACTTCTTTTT CAGAAGACAC CCTAAAAAAA GAAAAGCAAG CTGCCTACAA TGCTGACTCA 
GGCCTAAAAG CCGAAGCTAA TGGAGACCTG AAGACTGTGT CCACAAAGGG CAAGACCACC 
ACTGCAGATA TCCACAGCTA CGGCAGCAGT GGTGGTGGTG GCAGTGGAGG AGGTGGCGGT 
GTTGGTGGCG CTGGCGGCGG CCCTTGGGGA CCAGCGCCAG CCTGGTGCCC CTGCGGCTCC 
TGCTGCAGCT GGTGGAAGTG GCTGCTGGGC CTGCTGCTCA CCTGGCTGCT ACTCCTGGGG 
CTGCTCTTCG GCCTCATTGC TCTGGCGGAG GAGGTGAGGA AGCTGAAGGC GCGTGTGGAT 
GAGCTGGAGA GGATCAGGAG GAGCATACTG CCCTATGGGG ACAGCATGGA TAGAATAGAA 
AAGGACCGCC TCCAGGGCAT GGCACCCGCG GCGGGAGCAG ACCTGGACAA AATTGGGCTG 
CACAGTGACA GCCAGGAGGA GCTCTGGATG TTCGTGAGGA AGAAGCTAAT GATGGAACAG 
GAAAATGGAA ATCTCCGAGG AAGCCCTGGC CCTAAAGGTG ACATGGGAAG TCCAGGCCCT 
AAAGGAGATC GAGGGTTCCC TGGGACTCCA GGTATCCCTG GGCCCTTGGG CCACCCAGGT 
CCACAAGGAC CAAAGGGTCA AAAAGGCAGC GTGGGAGATC CTGGCATGGA AGGCCCCATG 
GGCCAGAGAG GGCGAGAAGG CCCCATGGGA CCTCGTGGTG AGGCAGGGCC TCCTGGATCT 
GGAGAGAAAG GGGAAAGAGG GGCTGCTGGT GAACCAGGTC CTCATGGCCC ACCTGGTGTC 
CCAGGTTCTG TGGGTCCCAA AGGTTCCAGC GGCTCTCCTG GCCCACAGGG CCCTCCAGGT 
CCTGTAGGTC TCCAAGGGCT CCGAGGTGAA GTAGGACTTC CTGGTGTCAA AGGTGACAAA 
GGACCAATGG GACCACCAGG ACCCAAAGGT GACCAGGGTG AGAAAGGACC TCGAGGCCTC 
ACAGGCGAGC CTGGCATGAG AGGTTTGCCT GGTGCTGTTG GTGAGCCCGG GGCTAAAGGA 
GCAATGGGTC CTGCTGGCCC AGACGGACAC CAAGGCCCAA GAGGTGAACA AGGTCTTACT 
GGGATGCCTG GAATCCGTGG CCCACCAGGA CCTTCTGGAG ACCCAGGAAA GCCAGGTCTC 
ACAGGACCCC AGGGACCTCA GGGACTTCCC GGTACCCCTG GCCGACCAGG AATAAAAGGT 
GAACCAGGAG CTCCAGGCAA GATCGTGACT TCGGAGGGGT CATCGATGCT CACTGTCCCA 
GGCCCCCCAG GACCTCCTGG AGCCATGGGA CCCCCAGGAC CTCCAGGTGC CCCAGGCCCT 
GCCGGCCCAG CTGGTCTCCC AGGACATCAA GAAGTTCTTA ATTTACAAGG TCCCCCAGGC 
CCACCCGGCC CACGCGGGCC ACCAGGGCCT TCCATTCCAG GCCCACCAGG ACCCCGAGGC 
CCACCAGGGG AGGGTTTGCC AGGCCCACCA GGCCCACCAG GATCGTTCCT GTCCAACTCA 
GAAACCTTCC TCTCCGGCCC CCCAGGCCCA CCTGGCCCCC CAGGTCCCAA GGGAGACCAA 
GGTCCCCCAG GCCCCAGAGG ACACCAAGGC GAGCAAGGCC TCCCAGGTTT CTCAACCTCA 
GGGTCCAGTT CTTTCGGACT CAACCTTCAG GGACCACCAG GCCCACCTGG CCCCCAGGGA 
CCCAAAGGTG ACAAAGGTGA TCCAGGTGTT CCAGGGGCTC TTGGCATTCC TAGTGGTCCT 
TCTGAAGGGG GATCATCAAG TACCATGTAC GTGTCAGGCC CGCCAGGGCC CCCTGGGCCC 
CCTGGGCCTC CGGGCTCTAT CAGCAGCTCT GGCCAGGAGA TTCAGCAGTA CATCTCTGAG 
TACATGCAGA GTGACAGTAT TAGATCTTAC CTATCCGGAG TTCAGGGTCC CCCAGGCCCA 
CCTGGTCCCC CAGGACCTGT CACCACCATC ACAGGCGAGA CTTTCGACTA CTCAGAGCTG 
GCAAGCCACG TTGTGAGCTA CTTACGGACT TCGGGGTACG GTGTCAGCTT GTTCTCGTCC 
TCCATCTCTT CTGAAGACAT TCTGGCTGTG CTGCAGCGGG ATGACGTGCG TCAGTACCTA 
CGTCAGTACT TGATGGGCCC TCGGGGTCCG CCAGGGCCAC CAGGAGCCAG TGGAGATGGG 
TCCCTCCTGT CTTTGGACTA TGCAGAGCTG AGTAGTCGCA TTCTCAGCTA CATGTCGAGT 
TCTGGGATCA GCATTGGGCT TCCTGGTCCC CCGGGGCCCC CTGGCTTGCC GGGAACCTCC 
TATGAGGAGC TCCTCTCCTT GCTGCGAGGG TCTGAATTCA GAGGCATCGT TGGACCCCCA 
GGTCCCCCGG GTCCACCAGG GATCCCAGGC AATGTGTGGT CCAGCATCAG CGTGGAGGAC 
CTCTCGTCTT ACTTACATAC TGCCGGCTTG TCATTCATCC CAGGCCCTCC AGGACCTCCT 
GGTCCCCCAG GGCCTCGAGG GCCCCCGGGT GTCTCAGGAG CCCTGGCAAC CTATGCAGCT 
GAAAACAGCG ACAGCTTCCG GAGCGAGCTG ATCAGCTACC TCACAAGTCC TGATGTGCGC 
AGCTTCATTG TTGGCCCCCC AGGCCCTCCT GGGCCGCAGG GACCCCCTGG GGACAGCCGC 
CTCCTGTCCA CGGATGCCTC CCACAGTCGG GGTAGCAGCT CCTCCTCACA CAGCTCATCT 
GTCAGGCGGG GCAGCTCCTA CAGCTCTTCC ATGAGCACAG GAGGAGGTGG TGCAGGCTCC 
CTGGGTGCAG GCGGTGCCTT TGGTGAAGCT GCAGGAGACA GGGGTCCCTA TGGCACTGAC 
ATCGGCCCAG GCGGAGGCTA TGGGGCAGCA GCAGAAGGCG GCATGTATGC TGGCAATGGC 
GGACTATTGG GAGCTGACTT TGCTGGAGAT CTGGATTACA ATGAGCTGGC TGTGAGGGTG 
TCAGAGAGCA TGCAGCGTCA GGGCCTACTG CAAGGGATGG CCTACACTGT CCAGGGCCCA 
CCAGGCCAGC CTGGGCCACA GGGGCCACCC GGCATCAGCA AGGTCTTCTC TGCCTACAGC 
AACGTGACTG CGGACCTCAT GGACTTCTTC CAAACTTATG GAGCCATTCA AGGACCCCCT 
GGGCAAAAAG GAGAGATGGG CACTCCAGGA CCCAAAGGTG ACAGGGGCCC TGCTGGGCCA 
CCAGGTCATC CTGGGCCACC TGGCCCTCGA GGACACAAGG GAGAAAAAGG AGACAAAGGT 
GACCAAGTCT ATGCTGGGCG GAGAAGGAGA AGAAGTATTG CTGTCAAGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL17A1 ( NM_000494.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000485.3 Homo sapiens collagen type XVII alpha 1 chain (COL17A1), mRNA.

Price & Availability↑

CloneID	OHu22348
Clone ID Related Accession (Same CDS sequence)	NM_000494.4 , NM_000494.3
Accession Version	NM_000494.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	collagen alpha-1(XVII) chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL138761.12. This sequence is a reference standard in the RefSeqGene project. On Dec 21, 2006 this sequence version replaced NM_000494.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M91669.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGATGTAA CCAAGAAAAA CAAACGAGAT GGAACTGAAG TCACTGAGAG AATTGTCACT 
GAAACAGTAA CCACAAGACT TACATCCTTA CCACCAAAAG GCGGGACCAG CAATGGCTAT 
GCTAAAACAG CCTCTCTTGG TGGAGGGAGC CGGCTGGAGA AACAAAGCCT GACTCATGGC 
AGCAGCGGCT ACATAAACTC AACTGGAAGC ACACGAGGCC ATGCCTCCAC CTCTAGTTAC 
AGGAGGGCTC ACTCACCTGC CTCCACTCTG CCCAACTCCC CAGGCTCAAC CTTTGAAAGG 
AAAACTCACG TTACCCGCCA TGCGTATGAA GGGAGCTCCA GTGGCAACTC TTCTCCGGAG 
TACCCTCGGA AGGAATTTGC ATCTTCTTCA ACCAGAGGAC GGAGTCAAAC ACGAGAGAGT 
GAAATTCGAG TTCGACTGCA GAGTGCGTCC CCATCCACCC GATGGACAGA ATTGGATGAT 
GTTAAGCGTT TGCTCAAGGG GAGTCGATCG GCAAGTGTGA GCCCCACCCG GAATTCCTCC 
AACACACTCC CCATCCCCAA GAAAGGCACT GTGGAGACCA AAATTGTGAC AGCGAGCTCC 
CAGTCGGTGT CAGGCACCTA CGATGCAACG ATCCTGGATG CCAACCTTCC CTCCCATGTG 
TGGTCCTCCA CCCTGCCCGC GGGGTCCTCC ATGGGGACCT ATCACAACAA CATGACAACC 
CAGAGCTCAT CCCTCCTCAA CACCAATGCC TACTCTGCGG GATCAGTCTT CGGAGTTCCA 
AACAACATGG CGTCCTGCTC ACCCACTTTG CACCCTGGAC TCAGCACATC CTCCTCAGTG 
TTTGGCATGC AGAACAATCT GGCCCCCAGC TTGACCACCC TGTCCCATGG CACCACCACC 
ACTTCCACAG CATATGGGGT GAAGAAAAAC ATGCCCCAGA GTCCTGCGGC TGTGAACACT 
GGCGTTTCCA CCTCCGCCGC CTGCACCACA AGTGTGCAGA GCGATGACCT TTTGCACAAG 
GACTGCAAGT TCCTGATCCT AGAGAAAGAC AACACACCTG CCAAGAAGGA GATGGAGCTG 
CTCATCATGA CCAAGGACAG CGGGAAGGTC TTTACAGCCT CCCCTGCCAG CATCGCTGCA 
ACTTCTTTTT CAGAAGACAC CCTAAAAAAA GAAAAGCAAG CTGCCTACAA TGCTGACTCA 
GGCCTAAAAG CCGAAGCTAA TGGAGACCTG AAGACTGTGT CCACAAAGGG CAAGACCACC 
ACTGCAGATA TCCACAGCTA CGGCAGCAGT GGTGGTGGTG GCAGTGGAGG AGGTGGCGGT 
GTTGGTGGCG CTGGCGGCGG CCCTTGGGGA CCAGCGCCAG CCTGGTGCCC CTGCGGCTCC 
TGCTGCAGCT GGTGGAAGTG GCTGCTGGGC CTGCTGCTCA CCTGGCTGCT ACTCCTGGGG 
CTGCTCTTCG GCCTCATTGC TCTGGCGGAG GAGGTGAGGA AGCTGAAGGC GCGTGTGGAT 
GAGCTGGAGA GGATCAGGAG GAGCATACTG CCCTATGGGG ACAGCATGGA TAGAATAGAA 
AAGGACCGCC TCCAGGGCAT GGCACCCGCG GCGGGAGCAG ACCTGGACAA AATTGGGCTG 
CACAGTGACA GCCAGGAGGA GCTCTGGATG TTCGTGAGGA AGAAGCTAAT GATGGAACAG 
GAAAATGGAA ATCTCCGAGG AAGCCCTGGC CCTAAAGGTG ACATGGGAAG TCCAGGCCCT 
AAAGGAGATC GAGGGTTCCC TGGGACTCCA GGTATCCCTG GGCCCTTGGG CCACCCAGGT 
CCACAAGGAC CAAAGGGTCA AAAAGGCAGC GTGGGAGATC CTGGCATGGA AGGCCCCATG 
GGCCAGAGAG GGCGAGAAGG CCCCATGGGA CCTCGTGGTG AGGCAGGGCC TCCTGGATCT 
GGAGAGAAAG GGGAAAGAGG GGCTGCTGGT GAACCAGGTC CTCATGGCCC ACCTGGTGTC 
CCAGGTTCTG TGGGTCCCAA AGGTTCCAGC GGCTCTCCTG GCCCACAGGG CCCTCCAGGT 
CCTGTAGGTC TCCAAGGGCT CCGAGGTGAA GTAGGACTTC CTGGTGTCAA AGGTGACAAA 
GGACCAATGG GACCACCAGG ACCCAAAGGT GACCAGGGTG AGAAAGGACC TCGAGGCCTC 
ACAGGCGAGC CTGGCATGAG AGGTTTGCCT GGTGCTGTTG GTGAGCCCGG GGCTAAAGGA 
GCAATGGGTC CTGCTGGCCC AGACGGACAC CAAGGCCCAA GAGGTGAACA AGGTCTTACT 
GGGATGCCTG GAATCCGTGG CCCACCAGGA CCTTCTGGAG ACCCAGGAAA GCCAGGTCTC 
ACAGGACCCC AGGGACCTCA GGGACTTCCC GGTACCCCTG GCCGACCAGG AATAAAAGGT 
GAACCAGGAG CTCCAGGCAA GATCGTGACT TCGGAGGGGT CATCGATGCT CACTGTCCCA 
GGCCCCCCAG GACCTCCTGG AGCCATGGGA CCCCCAGGAC CTCCAGGTGC CCCAGGCCCT 
GCCGGCCCAG CTGGTCTCCC AGGACATCAA GAAGTTCTTA ATTTACAAGG TCCCCCAGGC 
CCACCCGGCC CACGCGGGCC ACCAGGGCCT TCCATTCCAG GCCCACCAGG ACCCCGAGGC 
CCACCAGGGG AGGGTTTGCC AGGCCCACCA GGCCCACCAG GATCGTTCCT GTCCAACTCA 
GAAACCTTCC TCTCCGGCCC CCCAGGCCCA CCTGGCCCCC CAGGTCCCAA GGGAGACCAA 
GGTCCCCCAG GCCCCAGAGG ACACCAAGGC GAGCAAGGCC TCCCAGGTTT CTCAACCTCA 
GGGTCCAGTT CTTTCGGACT CAACCTTCAG GGACCACCAG GCCCACCTGG CCCCCAGGGA 
CCCAAAGGTG ACAAAGGTGA TCCAGGTGTT CCAGGGGCTC TTGGCATTCC TAGTGGTCCT 
TCTGAAGGGG GATCATCAAG TACCATGTAC GTGTCAGGCC CGCCAGGGCC CCCTGGGCCC 
CCTGGGCCTC CGGGCTCTAT CAGCAGCTCT GGCCAGGAGA TTCAGCAGTA CATCTCTGAG 
TACATGCAGA GTGACAGTAT TAGATCTTAC CTATCCGGAG TTCAGGGTCC CCCAGGCCCA 
CCTGGTCCCC CAGGACCTGT CACCACCATC ACAGGCGAGA CTTTCGACTA CTCAGAGCTG 
GCAAGCCACG TTGTGAGCTA CTTACGGACT TCGGGGTACG GTGTCAGCTT GTTCTCGTCC 
TCCATCTCTT CTGAAGACAT TCTGGCTGTG CTGCAGCGGG ATGACGTGCG TCAGTACCTA 
CGTCAGTACT TGATGGGCCC TCGGGGTCCG CCAGGGCCAC CAGGAGCCAG TGGAGATGGG 
TCCCTCCTGT CTTTGGACTA TGCAGAGCTG AGTAGTCGCA TTCTCAGCTA CATGTCGAGT 
TCTGGGATCA GCATTGGGCT TCCTGGTCCC CCGGGGCCCC CTGGCTTGCC GGGAACCTCC 
TATGAGGAGC TCCTCTCCTT GCTGCGAGGG TCTGAATTCA GAGGCATCGT TGGACCCCCA 
GGTCCCCCGG GTCCACCAGG GATCCCAGGC AATGTGTGGT CCAGCATCAG CGTGGAGGAC 
CTCTCGTCTT ACTTACATAC TGCCGGCTTG TCATTCATCC CAGGCCCTCC AGGACCTCCT 
GGTCCCCCAG GGCCTCGAGG GCCCCCGGGT GTCTCAGGAG CCCTGGCAAC CTATGCAGCT 
GAAAACAGCG ACAGCTTCCG GAGCGAGCTG ATCAGCTACC TCACAAGTCC TGATGTGCGC 
AGCTTCATTG TTGGCCCCCC AGGCCCTCCT GGGCCGCAGG GACCCCCTGG GGACAGCCGC 
CTCCTGTCCA CGGATGCCTC CCACAGTCGG GGTAGCAGCT CCTCCTCACA CAGCTCATCT 
GTCAGGCGGG GCAGCTCCTA CAGCTCTTCC ATGAGCACAG GAGGAGGTGG TGCAGGCTCC 
CTGGGTGCAG GCGGTGCCTT TGGTGAAGCT GCAGGAGACA GGGGTCCCTA TGGCACTGAC 
ATCGGCCCAG GCGGAGGCTA TGGGGCAGCA GCAGAAGGCG GCATGTATGC TGGCAATGGC 
GGACTATTGG GAGCTGACTT TGCTGGAGAT CTGGATTACA ATGAGCTGGC TGTGAGGGTG 
TCAGAGAGCA TGCAGCGTCA GGGCCTACTG CAAGGGATGG CCTACACTGT CCAGGGCCCA 
CCAGGCCAGC CTGGGCCACA GGGGCCACCC GGCATCAGCA AGGTCTTCTC TGCCTACAGC 
AACGTGACTG CGGACCTCAT GGACTTCTTC CAAACTTATG GAGCCATTCA AGGACCCCCT 
GGGCAAAAAG GAGAGATGGG CACTCCAGGA CCCAAAGGTG ACAGGGGCCC TGCTGGGCCA 
CCAGGTCATC CTGGGCCACC TGGCCCTCGA GGACACAAGG GAGAAAAAGG AGACAAAGGT 
GACCAAGTCT ATGCTGGGCG GAGAAGGAGA AGAAGTATTG CTGTCAAGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000485.3
CDS	170..4663
Translation	MDVTKKNKRDGTEVTERIVTETVTTRLTSLPPKGGTSNGYAKTASLGGGSRLEKQSLTHGSSGYINSTGSTRGHASTSSYRRAHSPASTLPNSPGSTFERKTHVTRHAYEGSSSGNSSPEYPRKEFASSSTRGRSQTRESEIRVRLQSASPSTRWTELDDVKRLLKGSRSASVSPTRNSSNTLPIPKKGTVETKIVTASSQSVSGTYDATILDANLPSHVWSSTLPAGSSMGTYHNNMTTQSSSLLNTNAYSAGSVFGVPNNMASCSPTLHPGLSTSSSVFGMQNNLAPSLTTLSHGTTTTSTAYGVKKNMPQSPAAVNTGVSTSAACTTSVQSDDLLHKDCKFLILEKDNTPAKKEMELLIMTKDSGKVFTASPASIAATSFSEDTLKKEKQAAYNADSGLKAEANGDLKTVSTKGKTTTADIHSYGSSGGGGSGGGGGVGGAGGGPWGPAPAWCPCGSCCSWWKWLLGLLLTWLLLLGLLFGLIALAEEVRKLKARVDELERIRRSILPYGDSMDRIEKDRLQGMAPAAGADLDKIGLHSDSQEELWMFVRKKLMMEQENGNLRGSPGPKGDMGSPGPKGDRGFPGTPGIPGPLGHPGPQGPKGQKGSVGDPGMEGPMGQRGREGPMGPRGEAGPPGSGEKGERGAAGEPGPHGPPGVPGSVGPKGSSGSPGPQGPPGPVGLQGLRGEVGLPGVKGDKGPMGPPGPKGDQGEKGPRGLTGEPGMRGLPGAVGEPGAKGAMGPAGPDGHQGPRGEQGLTGMPGIRGPPGPSGDPGKPGLTGPQGPQGLPGTPGRPGIKGEPGAPGKIVTSEGSSMLTVPGPPGPPGAMGPPGPPGAPGPAGPAGLPGHQEVLNLQGPPGPPGPRGPPGPSIPGPPGPRGPPGEGLPGPPGPPGSFLSNSETFLSGPPGPPGPPGPKGDQGPPGPRGHQGEQGLPGFSTSGSSSFGLNLQGPPGPPGPQGPKGDKGDPGVPGALGIPSGPSEGGSSSTMYVSGPPGPPGPPGPPGSISSSGQEIQQYISEYMQSDSIRSYLSGVQGPPGPPGPPGPVTTITGETFDYSELASHVVSYLRTSGYGVSLFSSSISSEDILAVLQRDDVRQYLRQYLMGPRGPPGPPGASGDGSLLSLDYAELSSRILSYMSSSGISIGLPGPPGPPGLPGTSYEELLSLLRGSEFRGIVGPPGPPGPPGIPGNVWSSISVEDLSSYLHTAGLSFIPGPPGPPGPPGPRGPPGVSGALATYAAENSDSFRSELISYLTSPDVRSFIVGPPGPPGPQGPPGDSRLLSTDASHSRGSSSSSHSSSVRRGSSYSSSMSTGGGGAGSLGAGGAFGEAAGDRGPYGTDIGPGGGYGAAAEGGMYAGNGGLLGADFAGDLDYNELAVRVSESMQRQGLLQGMAYTVQGPPGQPGPQGPPGISKVFSAYSNVTADLMDFFQTYGAIQGPPGQKGEMGTPGPKGDRGPAGPPGHPGPPGPRGHKGEKGDKGDQVYAGRRRRRSIAVKP

Target ORF information:

RefSeq Version	NM_000494.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type XVII alpha 1 chain (COL17A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000494.3

ORF Insert Sequence:

ATGGATGTAA CCAAGAAAAA CAAACGAGAT GGAACTGAAG TCACTGAGAG AATTGTCACT 
GAAACAGTAA CCACAAGACT TACATCCTTA CCACCAAAAG GCGGGACCAG CAATGGCTAT 
GCTAAAACAG CCTCTCTTGG TGGAGGGAGC CGGCTGGAGA AACAAAGCCT GACTCATGGC 
AGCAGCGGCT ACATAAACTC AACTGGAAGC ACACGAGGCC ATGCCTCCAC CTCTAGTTAC 
AGGAGGGCTC ACTCACCTGC CTCCACTCTG CCCAACTCCC CAGGCTCAAC CTTTGAAAGG 
AAAACTCACG TTACCCGCCA TGCGTATGAA GGGAGCTCCA GTGGCAACTC TTCTCCGGAG 
TACCCTCGGA AGGAATTTGC ATCTTCTTCA ACCAGAGGAC GGAGTCAAAC ACGAGAGAGT 
GAAATTCGAG TTCGACTGCA GAGTGCGTCC CCATCCACCC GATGGACAGA ATTGGATGAT 
GTTAAGCGTT TGCTCAAGGG GAGTCGATCG GCAAGTGTGA GCCCCACCCG GAATTCCTCC 
AACACACTCC CCATCCCCAA GAAAGGCACT GTGGAGACCA AAATTGTGAC AGCGAGCTCC 
CAGTCGGTGT CAGGCACCTA CGATGCAACG ATCCTGGATG CCAACCTTCC CTCCCATGTG 
TGGTCCTCCA CCCTGCCCGC GGGGTCCTCC ATGGGGACCT ATCACAACAA CATGACAACC 
CAGAGCTCAT CCCTCCTCAA CACCAATGCC TACTCTGCGG GATCAGTCTT CGGAGTTCCA 
AACAACATGG CGTCCTGCTC ACCCACTTTG CACCCTGGAC TCAGCACATC CTCCTCAGTG 
TTTGGCATGC AGAACAATCT GGCCCCCAGC TTGACCACCC TGTCCCATGG CACCACCACC 
ACTTCCACAG CATATGGGGT GAAGAAAAAC ATGCCCCAGA GTCCTGCGGC TGTGAACACT 
GGCGTTTCCA CCTCCGCCGC CTGCACCACA AGTGTGCAGA GCGATGACCT TTTGCACAAG 
GACTGCAAGT TCCTGATCCT AGAGAAAGAC AACACACCTG CCAAGAAGGA GATGGAGCTG 
CTCATCATGA CCAAGGACAG CGGGAAGGTC TTTACAGCCT CCCCTGCCAG CATCGCTGCA 
ACTTCTTTTT CAGAAGACAC CCTAAAAAAA GAAAAGCAAG CTGCCTACAA TGCTGACTCA 
GGCCTAAAAG CCGAAGCTAA TGGAGACCTG AAGACTGTGT CCACAAAGGG CAAGACCACC 
ACTGCAGATA TCCACAGCTA CGGCAGCAGT GGTGGTGGTG GCAGTGGAGG AGGTGGCGGT 
GTTGGTGGCG CTGGCGGCGG CCCTTGGGGA CCAGCGCCAG CCTGGTGCCC CTGCGGCTCC 
TGCTGCAGCT GGTGGAAGTG GCTGCTGGGC CTGCTGCTCA CCTGGCTGCT ACTCCTGGGG 
CTGCTCTTCG GCCTCATTGC TCTGGCGGAG GAGGTGAGGA AGCTGAAGGC GCGTGTGGAT 
GAGCTGGAGA GGATCAGGAG GAGCATACTG CCCTATGGGG ACAGCATGGA TAGAATAGAA 
AAGGACCGCC TCCAGGGCAT GGCACCCGCG GCGGGAGCAG ACCTGGACAA AATTGGGCTG 
CACAGTGACA GCCAGGAGGA GCTCTGGATG TTCGTGAGGA AGAAGCTAAT GATGGAACAG 
GAAAATGGAA ATCTCCGAGG AAGCCCTGGC CCTAAAGGTG ACATGGGAAG TCCAGGCCCT 
AAAGGAGATC GAGGGTTCCC TGGGACTCCA GGTATCCCTG GGCCCTTGGG CCACCCAGGT 
CCACAAGGAC CAAAGGGTCA AAAAGGCAGC GTGGGAGATC CTGGCATGGA AGGCCCCATG 
GGCCAGAGAG GGCGAGAAGG CCCCATGGGA CCTCGTGGTG AGGCAGGGCC TCCTGGATCT 
GGAGAGAAAG GGGAAAGAGG GGCTGCTGGT GAACCAGGTC CTCATGGCCC ACCTGGTGTC 
CCAGGTTCTG TGGGTCCCAA AGGTTCCAGC GGCTCTCCTG GCCCACAGGG CCCTCCAGGT 
CCTGTAGGTC TCCAAGGGCT CCGAGGTGAA GTAGGACTTC CTGGTGTCAA AGGTGACAAA 
GGACCAATGG GACCACCAGG ACCCAAAGGT GACCAGGGTG AGAAAGGACC TCGAGGCCTC 
ACAGGCGAGC CTGGCATGAG AGGTTTGCCT GGTGCTGTTG GTGAGCCCGG GGCTAAAGGA 
GCAATGGGTC CTGCTGGCCC AGACGGACAC CAAGGCCCAA GAGGTGAACA AGGTCTTACT 
GGGATGCCTG GAATCCGTGG CCCACCAGGA CCTTCTGGAG ACCCAGGAAA GCCAGGTCTC 
ACAGGACCCC AGGGACCTCA GGGACTTCCC GGTACCCCTG GCCGACCAGG AATAAAAGGT 
GAACCAGGAG CTCCAGGCAA GATCGTGACT TCGGAGGGGT CATCGATGCT CACTGTCCCA 
GGCCCCCCAG GACCTCCTGG AGCCATGGGA CCCCCAGGAC CTCCAGGTGC CCCAGGCCCT 
GCCGGCCCAG CTGGTCTCCC AGGACATCAA GAAGTTCTTA ATTTACAAGG TCCCCCAGGC 
CCACCCGGCC CACGCGGGCC ACCAGGGCCT TCCATTCCAG GCCCACCAGG ACCCCGAGGC 
CCACCAGGGG AGGGTTTGCC AGGCCCACCA GGCCCACCAG GATCGTTCCT GTCCAACTCA 
GAAACCTTCC TCTCCGGCCC CCCAGGCCCA CCTGGCCCCC CAGGTCCCAA GGGAGACCAA 
GGTCCCCCAG GCCCCAGAGG ACACCAAGGC GAGCAAGGCC TCCCAGGTTT CTCAACCTCA 
GGGTCCAGTT CTTTCGGACT CAACCTTCAG GGACCACCAG GCCCACCTGG CCCCCAGGGA 
CCCAAAGGTG ACAAAGGTGA TCCAGGTGTT CCAGGGGCTC TTGGCATTCC TAGTGGTCCT 
TCTGAAGGGG GATCATCAAG TACCATGTAC GTGTCAGGCC CGCCAGGGCC CCCTGGGCCC 
CCTGGGCCTC CGGGCTCTAT CAGCAGCTCT GGCCAGGAGA TTCAGCAGTA CATCTCTGAG 
TACATGCAGA GTGACAGTAT TAGATCTTAC CTATCCGGAG TTCAGGGTCC CCCAGGCCCA 
CCTGGTCCCC CAGGACCTGT CACCACCATC ACAGGCGAGA CTTTCGACTA CTCAGAGCTG 
GCAAGCCACG TTGTGAGCTA CTTACGGACT TCGGGGTACG GTGTCAGCTT GTTCTCGTCC 
TCCATCTCTT CTGAAGACAT TCTGGCTGTG CTGCAGCGGG ATGACGTGCG TCAGTACCTA 
CGTCAGTACT TGATGGGCCC TCGGGGTCCG CCAGGGCCAC CAGGAGCCAG TGGAGATGGG 
TCCCTCCTGT CTTTGGACTA TGCAGAGCTG AGTAGTCGCA TTCTCAGCTA CATGTCGAGT 
TCTGGGATCA GCATTGGGCT TCCTGGTCCC CCGGGGCCCC CTGGCTTGCC GGGAACCTCC 
TATGAGGAGC TCCTCTCCTT GCTGCGAGGG TCTGAATTCA GAGGCATCGT TGGACCCCCA 
GGTCCCCCGG GTCCACCAGG GATCCCAGGC AATGTGTGGT CCAGCATCAG CGTGGAGGAC 
CTCTCGTCTT ACTTACATAC TGCCGGCTTG TCATTCATCC CAGGCCCTCC AGGACCTCCT 
GGTCCCCCAG GGCCTCGAGG GCCCCCGGGT GTCTCAGGAG CCCTGGCAAC CTATGCAGCT 
GAAAACAGCG ACAGCTTCCG GAGCGAGCTG ATCAGCTACC TCACAAGTCC TGATGTGCGC 
AGCTTCATTG TTGGCCCCCC AGGCCCTCCT GGGCCGCAGG GACCCCCTGG GGACAGCCGC 
CTCCTGTCCA CGGATGCCTC CCACAGTCGG GGTAGCAGCT CCTCCTCACA CAGCTCATCT 
GTCAGGCGGG GCAGCTCCTA CAGCTCTTCC ATGAGCACAG GAGGAGGTGG TGCAGGCTCC 
CTGGGTGCAG GCGGTGCCTT TGGTGAAGCT GCAGGAGACA GGGGTCCCTA TGGCACTGAC 
ATCGGCCCAG GCGGAGGCTA TGGGGCAGCA GCAGAAGGCG GCATGTATGC TGGCAATGGC 
GGACTATTGG GAGCTGACTT TGCTGGAGAT CTGGATTACA ATGAGCTGGC TGTGAGGGTG 
TCAGAGAGCA TGCAGCGTCA GGGCCTACTG CAAGGGATGG CCTACACTGT CCAGGGCCCA 
CCAGGCCAGC CTGGGCCACA GGGGCCACCC GGCATCAGCA AGGTCTTCTC TGCCTACAGC 
AACGTGACTG CGGACCTCAT GGACTTCTTC CAAACTTATG GAGCCATTCA AGGACCCCCT 
GGGCAAAAAG GAGAGATGGG CACTCCAGGA CCCAAAGGTG ACAGGGGCCC TGCTGGGCCA 
CCAGGTCATC CTGGGCCACC TGGCCCTCGA GGACACAAGG GAGAAAAAGG AGACAAAGGT 
GACCAAGTCT ATGCTGGGCG GAGAAGGAGA AGAAGTATTG CTGTCAAGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

C-Terminal Processing of Collagen XVII Induces Neoepitopes for Linear IgA Dermatosis Autoantibodies.
The Journal of investigative dermatology137(12)2552-2559(2017 Dec)
Toyonaga E,Nishie W,Izumi K,Natsuga K,Ujiie H,Iwata H,Yamagami J,Hirako Y,Sawamura D,Fujimoto W,Shimizu H

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COL17A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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