DIS3L2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIS3L2
Entrez Gene ID	129563
Full Name	DIS3 like 3'-5' exoribonuclease 2
Synonyms	FAM6A,PRLMNS,hDIS3L2
General protein information	Preferred Names DIS3 like 3'-5' exoribonuclease 2 Names DIS3-like exonuclease 2 DIS3 mitotic control homolog-like 2 family with sequence similarity 6, member A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q37.1
Summary	The protein encoded by this gene is similar in sequence to 3'/5' exonucleolytic subunits of the RNA exosome. The exosome is a large multimeric ribonucleotide complex responsible for degrading various RNA substrates. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Mar 2012].
Disorder MIM:	614184
Disorder Html:	Perlman syndrome, 267000 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_152383.4	NP_689596.4	DIS3-like exonuclease 2 isoform 1
NM_001257281.1	NP_001244210.1	DIS3-like exonuclease 2 isoform 2
NM_152383.5	NP_689596.4	DIS3-like exonuclease 2 isoform 1
NM_001257281.2	NP_001244210.1	DIS3-like exonuclease 2 isoform 2
NM_001257282.1	NP_001244211.1	DIS3-like exonuclease 2 isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIS3L2	NP_689596.4
Mus musculus (house mouse)	Dis3l2	NP_705758.1
Danio rerio (zebrafish)	LOC566106	XP_005163305.1
Oryza sativa (rice)	Os02g0754100	NP_001048152.1
Pan troglodytes (chimpanzee)	DIS3L2	XP_003309564.1
Canis lupus familiaris (dog)	DIS3L2	XP_852722.1
Bos taurus (cattle)	DIS3L2	NP_001179435.1
Arabidopsis thaliana (thale cress)	AT1G77680	NP_177891.1
Rattus norvegicus (Norway rat)	Dis3l2	NP_001102477.2
Gallus gallus (chicken)	DIS3L2	XP_422741.4
Caenorhabditis elegans (roundworm)	F48E8.6	NP_498160.2
Schizosaccharomyces pombe (fission yeast)	SPAC2C4.07c	NP_594510.1
Xenopus tropicalis (tropical clawed frog)	dis3l2	NP_001072804.1

Gene Ontology
Bibliography

Related articles in PubMed

Immunohistochemical localization of exoribonucleases (DIS3L2 and XRN1) in intranuclear inclusion body disease.
Mori F, Tanji K, Miki Y, Toyoshima Y, Sasaki H, Yoshida M, Kakita A, Takahashi H, Wakabayashi K
Neuroscience letters662389-394(2018 Jan)

Lin28a uses distinct mechanisms of binding to RNA and affects miRNA levels positively and negatively.
Nowak JS, Hobor F, Downie Ruiz Velasco A, Choudhury NR, Heikel G, Kerr A, Ramos A, Michlewski G
RNA (New York, N.Y.)23(3)317-332(2017 03)

Perlman syndrome nuclease DIS3L2 controls cytoplasmic non-coding RNAs and provides surveillance pathway for maturing snRNAs.
?abno A, Warkocki Z, Kuli?ski T, Krawczyk PS, Bijata K, Tomecki R, Dziembowski A
Nucleic acids research44(21)10437-10453(2016 Dec)

The roles of the exoribonucleases DIS3L2 and XRN1 in human disease.
Pashler AL, Towler BP, Jones CI, Newbury SF
Biochemical Society transactions44(5)1377-1384(2016 10)

TUT-DIS3L2 is a mammalian surveillance pathway for aberrant structured non-coding RNAs.
Ustianenko D, Pasulka J, Feketova Z, Bednarik L, Zigackova D, Fortova A, Zavolan M, Vanacova S
The EMBO journal35(20)2179-2191(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The evidence has been presented that Dis3l2 controls miRNA-9 production.
Title: Lin28a uses distinct mechanisms of binding to RNA and affects miRNA levels positively and negatively.

these results indicate that catalytically inactive DIS3L2, characteristic of Perlman syndrome, can lead to deregulation of its target RNAs to disturb transcriptome homeostasis.
Title: Perlman syndrome nuclease DIS3L2 controls cytoplasmic non-coding RNAs and provides surveillance pathway for maturing snRNAs.

Studies indicate important roles of the exoribonucleases DIS3L2 and XRN1 in cellular function, viability and disease.
Title: The roles of the exoribonucleases DIS3L2 and XRN1 in human disease.

Our findings establish the role of DIS3L2 and oligouridylation as the cytoplasmic quality control for highly structured ncRNAs
Title: TUT-DIS3L2 is a mammalian surveillance pathway for aberrant structured non-coding RNAs.

DIS3L2 interacts with Ago2 and governs target RNA-directed miRNA degradation.
Title: Identification of factors involved in target RNA-directed microRNA degradation.

The following DIS3L2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIS3L2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18872	NM_152383.5 Latest version!	Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$444.50-$622.30 $889.00
OHu20305	NM_001257281.2 Latest version!	Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu22619	NM_001257282.1 Latest version!	Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu18872	NM_152383.4	Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$444.50-$622.30 $889.00
OHu20305	NM_001257281.1	Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIS3L2 ( NM_152383.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_689596.4 Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18872
Clone ID Related Accession (Same CDS sequence)	NM_152383.4 , NM_152383.5
Accession Version	NM_152383.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	DIS3-like exonuclease 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA058505.1, BC036113.1, BG746662.1, BM128478.1 and AA781347.1. This sequence is a reference standard in the RefSeqGene project. On Jun 5, 2007 this sequence version replaced NM_152383.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This sequence is assembled by in silico methods with additional support of similarity to proteins contained in mouse BC036177.1 and Xenopus BC121425.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.85156.1, SRR1660809.54811.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCCATC CTGACTACAG AATGAACCTC CGGCCCCTGG GGACCCCCAG AGGTGTGTCT 
GCTGTGGCTG GTCCACATGA CATTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAGG 
TCCACACGAG GGAAGAAAAA GAGCATATTT GAAACTTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAGAGGAAC ACTCATCCAG GGTGTATTGA GAATTAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC CCCGGATGGT GATCGAGACA TTTTTATTGA TGGGGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGATCTG GTGGTCGTGA AACTGCTTCC CGAGGAGCAT 
TGGAAGGTAG TTAAACCAGA GAGCAATGAC AAAGAAACAG AAGCTGCGTA TGAATCAGAT 
ATCCCCGAGG AGCTCTGTGG ACACCATCTC CCGCAACAGT CCCTGAAAAG CTATAATGAC 
AGTCCTGATG TCATTGTAGA GGCTCAGTTT GATGGCAGCG ACTCAGAAGA TGGACATGGC 
ATCACACAAA ATGTGCTGGT TGATGGTGTT AAGAAACTCT CAGTTTGTGT TTCTGAGAAA 
GGAAGAGAGG ATGGTGATGC ACCGGTTACA AAAGATGAGA CCACCTGCAT TTCACAAGAC 
ACAAGAGCTT TATCGGAGAA ATCCCTGCAA AGATCAGCAA AGGTGGTTTA CATCTTGGAG 
AAAAAACATT CTCGAGCAGC AACCGGCTTC CTCAAACTCT TGGCTGATAA GAACAGCGAA 
CTGTTTAGGA AATACGCCCT GTTTTCTCCC TCAGACCACC GAGTGCCTAG AATTTATGTG 
CCTCTCAAGG ACTGTCCCCA GGACTTTGTG GCACGGCCTA AAGATTATGC CAACACACTG 
TTCATCTGCC GCATTGTGGA CTGGAAGGAG GACTGCAATT TTGCCCTGGG GCAGCTGGCT 
AAGAGTCTTG GGCAGGCTGG TGAAATTGAG CCTGAAACAG AAGGAATACT AACAGAGTAT 
GGCGTGGATT TCTCTGATTT CTCTTCAGAA GTTCTAGAAT GTCTTCCTCA AGGCCTGCCA 
TGGACAATTC CACCAGAGGA GTTCAGCAAG AGAAGGGATT TAAGAAAAGA CTGTATCTTC 
ACCATTGACC CATCAACCGC CCGAGACCTC GATGATGCCC TCTCCTGCAA GCCACTCGCT 
GACGGCAACT TCAAAGTGGG AGTTCACATT GCTGACGTGA GTTACTTTGT TCCGGAGGGA 
TCTGATCTGG ATAAAGTGGC TGCCGAGAGG GCTACAAGCG TCTACTTGGT TCAAAAGGTG 
GTCCCCATGC TTCCCAGGCT GCTGTGTGAG GAGCTGTGCA GCCTCAACCC CATGTCCGAC 
AAGCTGACCT TCTCTGTGAT CTGGACACTG ACTCCAGAGG GCAAGATCCT TGATGAATGG 
TTTGGCCGGA CCATCATCCG CTCCTGCACC AAACTTAGCT ACGAGCATGC ACAGAGCATG 
ATTGAAAGCC CAACTGAGAA AATCCCTGCG AAAGAGCTGC CCCCCATTTC CCCAGAGCAT 
AGCAGCGAGG AGGTACACCA GGCCGTCTTG AATCTCCACG GAATTGCCAA GCAGTTACGC 
CAGCAGCGCT TTGTGGACGG CGCACTTCGT TTGGATCAGC TAAAGCTTGC TTTCACTCTG 
GACCACGAGA CCGGATTGCC TCAAGGATGT CATATCTATG AGTACCGCGA GAGCAACAAG 
CTCGTGGAGG AGTTCATGCT CTTGGCCAAC ATGGCAGTGG CCCACAAGAT CCACCGCGCC 
TTCCCCGAGC AGGCCCTGCT GCGCCGGCAC CCCCCGCCCC AAACAAGGAT GCTCAGTGAC 
CTGGTGGAAT TCTGCGACCA GATGGGGCTG CCCGTGGACT TCAGCTCCGC AGGAGCCCTC 
AATAAAAGCC TGACCCAAAC ATTTGGAGAT GACAAGTACT CACTGGCCCG CAAGGAGGTG 
CTCACCAACA TGTGCTCCCG GCCCATGCAG ATGGCACTGT ACTTCTGCTC GGGGCTGCTG 
CAGGACCCAG CGCAGTTCCG GCACTACGCG CTCAATGTGC CCCTGTACAC ACACTTCACC 
TCGCCCATCC GCCGCTTTGC CGACGTCCTG GTGCACCGCC TCCTGGCTGC CGCGTTAGGC 
TATAGGGAGC GACTAGACAT GGCGCCCGAT ACCCTGCAGA AACAGGCGGA CCACTGTAAC 
GACCGCCGCA TGGCGTCCAA GCGCGTGCAG GAGCTCAGTA CCAGTCTCTT CTTTGCTGTT 
CTGGTCAAGG AGAGTGGCCC CCTGGAGTCA GAAGCCATGG TGATGGGCAT CCTGAAGCAA 
GCCTTCGACG TGCTGGTGCT GCGCTACGGC GTGCAGAAGC GCATCTACTG CAACGCACTG 
GCCCTGCGGT CCCACCACTT CCAGAAGGTG GGCAAGAAGC CGGAACTCAC GCTGGTCTGG 
GAGCCTGAGG ACATGGAGCA GGAGCCAGCA CAGCAGGTCA TCACCATCTT CAGCCTGGTG 
GAGGTGGTCC TGCAGGCAGA GTCCACAGCC CTCAAGTACA GCGCCATCCT GAAGCGGCCA 
GGCACCCAGG GCCACCTGGG CCCTGAGAAG GAGGAGGAGG AGTCTGACGG TGAGCCCGAG 
GACTCAAGCA CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689596.4
CDS	277..2934
Translation	MSHPDYRMNLRPLGTPRGVSAVAGPHDIGASPGDKKSKNRSTRGKKKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRALNGDLVVVKLLPEEHWKVVKPESNDKETEAAYESDIPEELCGHHLPQQSLKSYNDSPDVIVEAQFDGSDSEDGHGITQNVLVDGVKKLSVCVSEKGREDGDAPVTKDETTCISQDTRALSEKSLQRSAKVVYILEKKHSRAATGFLKLLADKNSELFRKYALFSPSDHRVPRIYVPLKDCPQDFVARPKDYANTLFICRIVDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQGLPWTIPPEEFSKRRDLRKDCIFTIDPSTARDLDDALSCKPLADGNFKVGVHIADVSYFVPEGSDLDKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMSDKLTFSVIWTLTPEGKILDEWFGRTIIRSCTKLSYEHAQSMIESPTEKIPAKELPPISPEHSSEEVHQAVLNLHGIAKQLRQQRFVDGALRLDQLKLAFTLDHETGLPQGCHIYEYRESNKLVEEFMLLANMAVAHKIHRAFPEQALLRRHPPPQTRMLSDLVEFCDQMGLPVDFSSAGALNKSLTQTFGDDKYSLARKEVLTNMCSRPMQMALYFCSGLLQDPAQFRHYALNVPLYTHFTSPIRRFADVLVHRLLAAALGYRERLDMAPDTLQKQADHCNDRRMASKRVQELSTSLFFAVLVKESGPLESEAMVMGILKQAFDVLVLRYGVQKRIYCNALALRSHHFQKVGKKPELTLVWEPEDMEQEPAQQVITIFSLVEVVLQAESTALKYSAILKRPGTQGHLGPEKEEEESDGEPEDSSTS

Target ORF information:

RefSeq Version	NM_152383.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152383.4

ORF Insert Sequence:

ATGAGCCATC CTGACTACAG AATGAACCTC CGGCCCCTGG GGACCCCCAG AGGTGTGTCT 
GCTGTGGCTG GTCCACATGA CATTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAGG 
TCCACACGAG GGAAGAAAAA GAGCATATTT GAAACTTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAGAGGAAC ACTCATCCAG GGTGTATTGA GAATTAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC CCCGGATGGT GATCGAGACA TTTTTATTGA TGGGGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGATCTG GTGGTCGTGA AACTGCTTCC CGAGGAGCAT 
TGGAAGGTAG TTAAACCAGA GAGCAATGAC AAAGAAACAG AAGCTGCGTA TGAATCAGAT 
ATCCCCGAGG AGCTCTGTGG ACACCATCTC CCGCAACAGT CCCTGAAAAG CTATAATGAC 
AGTCCTGATG TCATTGTAGA GGCTCAGTTT GATGGCAGCG ACTCAGAAGA TGGACATGGC 
ATCACACAAA ATGTGCTGGT TGATGGTGTT AAGAAACTCT CAGTTTGTGT TTCTGAGAAA 
GGAAGAGAGG ATGGTGATGC ACCGGTTACA AAAGATGAGA CCACCTGCAT TTCACAAGAC 
ACAAGAGCTT TATCGGAGAA ATCCCTGCAA AGATCAGCAA AGGTGGTTTA CATCTTGGAG 
AAAAAACATT CTCGAGCAGC AACCGGCTTC CTCAAACTCT TGGCTGATAA GAACAGCGAA 
CTGTTTAGGA AATACGCCCT GTTTTCTCCC TCAGACCACC GAGTGCCTAG AATTTATGTG 
CCTCTCAAGG ACTGTCCCCA GGACTTTGTG GCACGGCCTA AAGATTATGC CAACACACTG 
TTCATCTGCC GCATTGTGGA CTGGAAGGAG GACTGCAATT TTGCCCTGGG GCAGCTGGCT 
AAGAGTCTTG GGCAGGCTGG TGAAATTGAG CCTGAAACAG AAGGAATACT AACAGAGTAT 
GGCGTGGATT TCTCTGATTT CTCTTCAGAA GTTCTAGAAT GTCTTCCTCA AGGCCTGCCA 
TGGACAATTC CACCAGAGGA GTTCAGCAAG AGAAGGGATT TAAGAAAAGA CTGTATCTTC 
ACCATTGACC CATCAACCGC CCGAGACCTC GATGATGCCC TCTCCTGCAA GCCACTCGCT 
GACGGCAACT TCAAAGTGGG AGTTCACATT GCTGACGTGA GTTACTTTGT TCCGGAGGGA 
TCTGATCTGG ATAAAGTGGC TGCCGAGAGG GCTACAAGCG TCTACTTGGT TCAAAAGGTG 
GTCCCCATGC TTCCCAGGCT GCTGTGTGAG GAGCTGTGCA GCCTCAACCC CATGTCCGAC 
AAGCTGACCT TCTCTGTGAT CTGGACACTG ACTCCAGAGG GCAAGATCCT TGATGAATGG 
TTTGGCCGGA CCATCATCCG CTCCTGCACC AAACTTAGCT ACGAGCATGC ACAGAGCATG 
ATTGAAAGCC CAACTGAGAA AATCCCTGCG AAAGAGCTGC CCCCCATTTC CCCAGAGCAT 
AGCAGCGAGG AGGTACACCA GGCCGTCTTG AATCTCCACG GAATTGCCAA GCAGTTACGC 
CAGCAGCGCT TTGTGGACGG CGCACTTCGT TTGGATCAGC TAAAGCTTGC TTTCACTCTG 
GACCACGAGA CCGGATTGCC TCAAGGATGT CATATCTATG AGTACCGCGA GAGCAACAAG 
CTCGTGGAGG AGTTCATGCT CTTGGCCAAC ATGGCAGTGG CCCACAAGAT CCACCGCGCC 
TTCCCCGAGC AGGCCCTGCT GCGCCGGCAC CCCCCGCCCC AAACAAGGAT GCTCAGTGAC 
CTGGTGGAAT TCTGCGACCA GATGGGGCTG CCCGTGGACT TCAGCTCCGC AGGAGCCCTC 
AATAAAAGCC TGACCCAAAC ATTTGGAGAT GACAAGTACT CACTGGCCCG CAAGGAGGTG 
CTCACCAACA TGTGCTCCCG GCCCATGCAG ATGGCACTGT ACTTCTGCTC GGGGCTGCTG 
CAGGACCCAG CGCAGTTCCG GCACTACGCG CTCAATGTGC CCCTGTACAC ACACTTCACC 
TCGCCCATCC GCCGCTTTGC CGACGTCCTG GTGCACCGCC TCCTGGCTGC CGCGTTAGGC 
TATAGGGAGC GACTAGACAT GGCGCCCGAT ACCCTGCAGA AACAGGCGGA CCACTGTAAC 
GACCGCCGCA TGGCGTCCAA GCGCGTGCAG GAGCTCAGTA CCAGTCTCTT CTTTGCTGTT 
CTGGTCAAGG AGAGTGGCCC CCTGGAGTCA GAAGCCATGG TGATGGGCAT CCTGAAGCAA 
GCCTTCGACG TGCTGGTGCT GCGCTACGGC GTGCAGAAGC GCATCTACTG CAACGCACTG 
GCCCTGCGGT CCCACCACTT CCAGAAGGTG GGCAAGAAGC CGGAACTCAC GCTGGTCTGG 
GAGCCTGAGG ACATGGAGCA GGAGCCAGCA CAGCAGGTCA TCACCATCTT CAGCCTGGTG 
GAGGTGGTCC TGCAGGCAGA GTCCACAGCC CTCAAGTACA GCGCCATCCT GAAGCGGCCA 
GGCACCCAGG GCCACCTGGG CCCTGAGAAG GAGGAGGAGG AGTCTGACGG TGAGCCCGAG 
GACTCAAGCA CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIS3L2 ( NM_152383.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_689596.4 Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18872
Clone ID Related Accession (Same CDS sequence)	NM_152383.4 , NM_152383.5
Accession Version	NM_152383.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	DIS3-like exonuclease 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA058505.1, BC036113.1, BG746662.1 and BM128478.1. On Dec 27, 2019 this sequence version replaced NM_152383.4. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This sequence is assembled by in silico methods with additional support of similarity to proteins contained in mouse BC036177.1 and Xenopus BC121425.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.130065.1, SRR3476690.339930.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCCATC CTGACTACAG AATGAACCTC CGGCCCCTGG GGACCCCCAG AGGTGTGTCT 
GCTGTGGCTG GTCCACATGA CATTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAGG 
TCCACACGAG GGAAGAAAAA GAGCATATTT GAAACTTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAGAGGAAC ACTCATCCAG GGTGTATTGA GAATTAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC CCCGGATGGT GATCGAGACA TTTTTATTGA TGGGGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGATCTG GTGGTCGTGA AACTGCTTCC CGAGGAGCAT 
TGGAAGGTAG TTAAACCAGA GAGCAATGAC AAAGAAACAG AAGCTGCGTA TGAATCAGAT 
ATCCCCGAGG AGCTCTGTGG ACACCATCTC CCGCAACAGT CCCTGAAAAG CTATAATGAC 
AGTCCTGATG TCATTGTAGA GGCTCAGTTT GATGGCAGCG ACTCAGAAGA TGGACATGGC 
ATCACACAAA ATGTGCTGGT TGATGGTGTT AAGAAACTCT CAGTTTGTGT TTCTGAGAAA 
GGAAGAGAGG ATGGTGATGC ACCGGTTACA AAAGATGAGA CCACCTGCAT TTCACAAGAC 
ACAAGAGCTT TATCGGAGAA ATCCCTGCAA AGATCAGCAA AGGTGGTTTA CATCTTGGAG 
AAAAAACATT CTCGAGCAGC AACCGGCTTC CTCAAACTCT TGGCTGATAA GAACAGCGAA 
CTGTTTAGGA AATACGCCCT GTTTTCTCCC TCAGACCACC GAGTGCCTAG AATTTATGTG 
CCTCTCAAGG ACTGTCCCCA GGACTTTGTG GCACGGCCTA AAGATTATGC CAACACACTG 
TTCATCTGCC GCATTGTGGA CTGGAAGGAG GACTGCAATT TTGCCCTGGG GCAGCTGGCT 
AAGAGTCTTG GGCAGGCTGG TGAAATTGAG CCTGAAACAG AAGGAATACT AACAGAGTAT 
GGCGTGGATT TCTCTGATTT CTCTTCAGAA GTTCTAGAAT GTCTTCCTCA AGGCCTGCCA 
TGGACAATTC CACCAGAGGA GTTCAGCAAG AGAAGGGATT TAAGAAAAGA CTGTATCTTC 
ACCATTGACC CATCAACCGC CCGAGACCTC GATGATGCCC TCTCCTGCAA GCCACTCGCT 
GACGGCAACT TCAAAGTGGG AGTTCACATT GCTGACGTGA GTTACTTTGT TCCGGAGGGA 
TCTGATCTGG ATAAAGTGGC TGCCGAGAGG GCTACAAGCG TCTACTTGGT TCAAAAGGTG 
GTCCCCATGC TTCCCAGGCT GCTGTGTGAG GAGCTGTGCA GCCTCAACCC CATGTCCGAC 
AAGCTGACCT TCTCTGTGAT CTGGACACTG ACTCCAGAGG GCAAGATCCT TGATGAATGG 
TTTGGCCGGA CCATCATCCG CTCCTGCACC AAACTTAGCT ACGAGCATGC ACAGAGCATG 
ATTGAAAGCC CAACTGAGAA AATCCCTGCG AAAGAGCTGC CCCCCATTTC CCCAGAGCAT 
AGCAGCGAGG AGGTACACCA GGCCGTCTTG AATCTCCACG GAATTGCCAA GCAGTTACGC 
CAGCAGCGCT TTGTGGACGG CGCACTTCGT TTGGATCAGC TAAAGCTTGC TTTCACTCTG 
GACCACGAGA CCGGATTGCC TCAAGGATGT CATATCTATG AGTACCGCGA GAGCAACAAG 
CTCGTGGAGG AGTTCATGCT CTTGGCCAAC ATGGCAGTGG CCCACAAGAT CCACCGCGCC 
TTCCCCGAGC AGGCCCTGCT GCGCCGGCAC CCCCCGCCCC AAACAAGGAT GCTCAGTGAC 
CTGGTGGAAT TCTGCGACCA GATGGGGCTG CCCGTGGACT TCAGCTCCGC AGGAGCCCTC 
AATAAAAGCC TGACCCAAAC ATTTGGAGAT GACAAGTACT CACTGGCCCG CAAGGAGGTG 
CTCACCAACA TGTGCTCCCG GCCCATGCAG ATGGCACTGT ACTTCTGCTC GGGGCTGCTG 
CAGGACCCAG CGCAGTTCCG GCACTACGCG CTCAATGTGC CCCTGTACAC ACACTTCACC 
TCGCCCATCC GCCGCTTTGC CGACGTCCTG GTGCACCGCC TCCTGGCTGC CGCGTTAGGC 
TATAGGGAGC GACTAGACAT GGCGCCCGAT ACCCTGCAGA AACAGGCGGA CCACTGTAAC 
GACCGCCGCA TGGCGTCCAA GCGCGTGCAG GAGCTCAGTA CCAGTCTCTT CTTTGCTGTT 
CTGGTCAAGG AGAGTGGCCC CCTGGAGTCA GAAGCCATGG TGATGGGCAT CCTGAAGCAA 
GCCTTCGACG TGCTGGTGCT GCGCTACGGC GTGCAGAAGC GCATCTACTG CAACGCACTG 
GCCCTGCGGT CCCACCACTT CCAGAAGGTG GGCAAGAAGC CGGAACTCAC GCTGGTCTGG 
GAGCCTGAGG ACATGGAGCA GGAGCCAGCA CAGCAGGTCA TCACCATCTT CAGCCTGGTG 
GAGGTGGTCC TGCAGGCAGA GTCCACAGCC CTCAAGTACA GCGCCATCCT GAAGCGGCCA 
GGCACCCAGG GCCACCTGGG CCCTGAGAAG GAGGAGGAGG AGTCTGACGG TGAGCCCGAG 
GACTCAAGCA CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689596.4
CDS	147..2804
Translation	MSHPDYRMNLRPLGTPRGVSAVAGPHDIGASPGDKKSKNRSTRGKKKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRALNGDLVVVKLLPEEHWKVVKPESNDKETEAAYESDIPEELCGHHLPQQSLKSYNDSPDVIVEAQFDGSDSEDGHGITQNVLVDGVKKLSVCVSEKGREDGDAPVTKDETTCISQDTRALSEKSLQRSAKVVYILEKKHSRAATGFLKLLADKNSELFRKYALFSPSDHRVPRIYVPLKDCPQDFVARPKDYANTLFICRIVDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQGLPWTIPPEEFSKRRDLRKDCIFTIDPSTARDLDDALSCKPLADGNFKVGVHIADVSYFVPEGSDLDKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMSDKLTFSVIWTLTPEGKILDEWFGRTIIRSCTKLSYEHAQSMIESPTEKIPAKELPPISPEHSSEEVHQAVLNLHGIAKQLRQQRFVDGALRLDQLKLAFTLDHETGLPQGCHIYEYRESNKLVEEFMLLANMAVAHKIHRAFPEQALLRRHPPPQTRMLSDLVEFCDQMGLPVDFSSAGALNKSLTQTFGDDKYSLARKEVLTNMCSRPMQMALYFCSGLLQDPAQFRHYALNVPLYTHFTSPIRRFADVLVHRLLAAALGYRERLDMAPDTLQKQADHCNDRRMASKRVQELSTSLFFAVLVKESGPLESEAMVMGILKQAFDVLVLRYGVQKRIYCNALALRSHHFQKVGKKPELTLVWEPEDMEQEPAQQVITIFSLVEVVLQAESTALKYSAILKRPGTQGHLGPEKEEEESDGEPEDSSTS

Target ORF information:

RefSeq Version	NM_152383.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152383.5

ORF Insert Sequence:

ATGAGCCATC CTGACTACAG AATGAACCTC CGGCCCCTGG GGACCCCCAG AGGTGTGTCT 
GCTGTGGCTG GTCCACATGA CATTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAGG 
TCCACACGAG GGAAGAAAAA GAGCATATTT GAAACTTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAGAGGAAC ACTCATCCAG GGTGTATTGA GAATTAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC CCCGGATGGT GATCGAGACA TTTTTATTGA TGGGGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGATCTG GTGGTCGTGA AACTGCTTCC CGAGGAGCAT 
TGGAAGGTAG TTAAACCAGA GAGCAATGAC AAAGAAACAG AAGCTGCGTA TGAATCAGAT 
ATCCCCGAGG AGCTCTGTGG ACACCATCTC CCGCAACAGT CCCTGAAAAG CTATAATGAC 
AGTCCTGATG TCATTGTAGA GGCTCAGTTT GATGGCAGCG ACTCAGAAGA TGGACATGGC 
ATCACACAAA ATGTGCTGGT TGATGGTGTT AAGAAACTCT CAGTTTGTGT TTCTGAGAAA 
GGAAGAGAGG ATGGTGATGC ACCGGTTACA AAAGATGAGA CCACCTGCAT TTCACAAGAC 
ACAAGAGCTT TATCGGAGAA ATCCCTGCAA AGATCAGCAA AGGTGGTTTA CATCTTGGAG 
AAAAAACATT CTCGAGCAGC AACCGGCTTC CTCAAACTCT TGGCTGATAA GAACAGCGAA 
CTGTTTAGGA AATACGCCCT GTTTTCTCCC TCAGACCACC GAGTGCCTAG AATTTATGTG 
CCTCTCAAGG ACTGTCCCCA GGACTTTGTG GCACGGCCTA AAGATTATGC CAACACACTG 
TTCATCTGCC GCATTGTGGA CTGGAAGGAG GACTGCAATT TTGCCCTGGG GCAGCTGGCT 
AAGAGTCTTG GGCAGGCTGG TGAAATTGAG CCTGAAACAG AAGGAATACT AACAGAGTAT 
GGCGTGGATT TCTCTGATTT CTCTTCAGAA GTTCTAGAAT GTCTTCCTCA AGGCCTGCCA 
TGGACAATTC CACCAGAGGA GTTCAGCAAG AGAAGGGATT TAAGAAAAGA CTGTATCTTC 
ACCATTGACC CATCAACCGC CCGAGACCTC GATGATGCCC TCTCCTGCAA GCCACTCGCT 
GACGGCAACT TCAAAGTGGG AGTTCACATT GCTGACGTGA GTTACTTTGT TCCGGAGGGA 
TCTGATCTGG ATAAAGTGGC TGCCGAGAGG GCTACAAGCG TCTACTTGGT TCAAAAGGTG 
GTCCCCATGC TTCCCAGGCT GCTGTGTGAG GAGCTGTGCA GCCTCAACCC CATGTCCGAC 
AAGCTGACCT TCTCTGTGAT CTGGACACTG ACTCCAGAGG GCAAGATCCT TGATGAATGG 
TTTGGCCGGA CCATCATCCG CTCCTGCACC AAACTTAGCT ACGAGCATGC ACAGAGCATG 
ATTGAAAGCC CAACTGAGAA AATCCCTGCG AAAGAGCTGC CCCCCATTTC CCCAGAGCAT 
AGCAGCGAGG AGGTACACCA GGCCGTCTTG AATCTCCACG GAATTGCCAA GCAGTTACGC 
CAGCAGCGCT TTGTGGACGG CGCACTTCGT TTGGATCAGC TAAAGCTTGC TTTCACTCTG 
GACCACGAGA CCGGATTGCC TCAAGGATGT CATATCTATG AGTACCGCGA GAGCAACAAG 
CTCGTGGAGG AGTTCATGCT CTTGGCCAAC ATGGCAGTGG CCCACAAGAT CCACCGCGCC 
TTCCCCGAGC AGGCCCTGCT GCGCCGGCAC CCCCCGCCCC AAACAAGGAT GCTCAGTGAC 
CTGGTGGAAT TCTGCGACCA GATGGGGCTG CCCGTGGACT TCAGCTCCGC AGGAGCCCTC 
AATAAAAGCC TGACCCAAAC ATTTGGAGAT GACAAGTACT CACTGGCCCG CAAGGAGGTG 
CTCACCAACA TGTGCTCCCG GCCCATGCAG ATGGCACTGT ACTTCTGCTC GGGGCTGCTG 
CAGGACCCAG CGCAGTTCCG GCACTACGCG CTCAATGTGC CCCTGTACAC ACACTTCACC 
TCGCCCATCC GCCGCTTTGC CGACGTCCTG GTGCACCGCC TCCTGGCTGC CGCGTTAGGC 
TATAGGGAGC GACTAGACAT GGCGCCCGAT ACCCTGCAGA AACAGGCGGA CCACTGTAAC 
GACCGCCGCA TGGCGTCCAA GCGCGTGCAG GAGCTCAGTA CCAGTCTCTT CTTTGCTGTT 
CTGGTCAAGG AGAGTGGCCC CCTGGAGTCA GAAGCCATGG TGATGGGCAT CCTGAAGCAA 
GCCTTCGACG TGCTGGTGCT GCGCTACGGC GTGCAGAAGC GCATCTACTG CAACGCACTG 
GCCCTGCGGT CCCACCACTT CCAGAAGGTG GGCAAGAAGC CGGAACTCAC GCTGGTCTGG 
GAGCCTGAGG ACATGGAGCA GGAGCCAGCA CAGCAGGTCA TCACCATCTT CAGCCTGGTG 
GAGGTGGTCC TGCAGGCAGA GTCCACAGCC CTCAAGTACA GCGCCATCCT GAAGCGGCCA 
GGCACCCAGG GCCACCTGGG CCCTGAGAAG GAGGAGGAGG AGTCTGACGG TGAGCCCGAG 
GACTCAAGCA CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIS3L2 ( NM_001257281.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244210.1 Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20305
Clone ID Related Accession (Same CDS sequence)	NM_001257281.1 , NM_001257281.2
Accession Version	NM_001257281.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	DIS3-like exonuclease 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK094293.1. Transcript Variant: This variant (2) lacks several exons and includes an alternate 3' terminal exon compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK094293.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAGCCATC CTGACTACAG AATGAACCTC CGGCCCCTGG GGACCCCCAG AGGTGTGTCT 
GCTGTGGCTG GTCCACATGA CATTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAGG 
TCCACACGAG GGAAGAAAAA GAGCATATTT GAAACTTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAGAGGAAC ACTCATCCAG GGTGTATTGA GAATTAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC CCCGGATGGT GATCGAGACA TTTTTATTGA TGGGGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGATCTG GTGGTCGTGA AACTGCTTCC CGAGGAGCAT 
TGGAAGGTAG TTAAACCAGA GAGCAATGAC AAAGAAACAG AAGCTGCGTA TGAATCAGAT 
ATCCCCGAGG AGCTCTGTGG ACACCATCTC CCGCAACAGT CCCTGAAAAG CTATAATGAC 
AGTCCTGATG TCATTGTAGA GGCTCAGTTT GATGGCAGCG ACTCAGAAGA TGGACATGGC 
ATCACACAAA ATGTGCTGGT TGATGGTGTT AAGAAACTCT CAGTTTGTGT TTCTGAGAAA 
GGAAGAGAGG ATGGTGATGC ACCGGTTACA AAAGATGAGA CCACCTGCAT TTCACAAGAC 
ACAAGAGCTT TATCGGAGAA ATCCCTGCAA AGATCAGCAA AGGTGGTTTA CATCTTGGAG 
AAAAAACATT CTCGAGCAGC AACCGGCTTC CTCAAACTCT TGGCTGATAA GAACAGCGAA 
CTGTTTAGGA AATACGCCCT GTTTTCTCCC TCAGACCACC GAGTGCCTAG AATTTATGTG 
CCTCTCAAGG ACTGTCCCCA GGACTTTGTG GCACGGCCTA AAGATTATGC CAACACACTG 
TTCATCTGCC GCATTGTGGA CTGGAAGGAG GACTGCAATT TTGCCCTGGG GCAGCTGGCT 
AAGAGTCTTG GGCAGGCTGG TGAAATTGAG CCTGAAACAG AAGGAATACT AACAGAGTAT 
GGCGTGGATT TCTCTGATTT CTCTTCAGAA GTTCTAGAAT GTCTTCCTCA AGGCCTGCCA 
TGGACAATTC CACCAGAGGA GTTCAGCAAG AGAAGGGATT TAAGAAAAGA CTGTATCTTC 
ACCATTGACC CATCAACCGC CCGAGACCTC GATGATGCCC TCTCCTGCAA GCCACTCGCT 
GACGGCAACT TCAAAGTGGG AGTTCACATT GCTGACGTGA GTTACTTTGT TCCGGAGGGA 
TCTGATCTGG ATAAAGTGGC TGCCGAGAGG GCTACAAGCG TCTACTTGGT TCAAAAGGTG 
GTCCCCATGC TTCCCAGGCT GCTGTGTGAG GAGCTGTGCA GCCTCAACCC CATGTCCGAC 
AAGCTGACCT TCTCTGTGAT CTGGACACTG ACTCCAGAGG GCAAGATCCT TGATGAATGG 
TTTGGCCGGA CCATCATCCG CTCCTGCACC AAACTTAGCT ACGAGCATGC ACAGAGCATG 
ATTGAAAGCC CAACTGAGAA AATCCCTGCG AAAGAGCTGC CCCCCATTTC CCCAGAGCAT 
AGCAGCGAGG AGGTACACCA GCAGAACGCA GACAAGGATG GGGCTGCCCA TCTTCAGGCC 
TCTCACAGCC CCTCTGCTGA AGATGCAGAA GCACAGCCCT CCACAGAGGA ACGCCTGCCT 
GAGACTCGGG GCATATGTGA CAGGGATCCA GACACACGAC TGTTTTTCCT TCAGCAACAG 
AGCCGTGTAT TGGAAGCAAA GCCCCAAAAC ACGATAAGAG TAGAGGAGCA GACAACCCAG 
TTGCAGATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244210.1
CDS	277..2088
Translation	MSHPDYRMNLRPLGTPRGVSAVAGPHDIGASPGDKKSKNRSTRGKKKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRALNGDLVVVKLLPEEHWKVVKPESNDKETEAAYESDIPEELCGHHLPQQSLKSYNDSPDVIVEAQFDGSDSEDGHGITQNVLVDGVKKLSVCVSEKGREDGDAPVTKDETTCISQDTRALSEKSLQRSAKVVYILEKKHSRAATGFLKLLADKNSELFRKYALFSPSDHRVPRIYVPLKDCPQDFVARPKDYANTLFICRIVDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQGLPWTIPPEEFSKRRDLRKDCIFTIDPSTARDLDDALSCKPLADGNFKVGVHIADVSYFVPEGSDLDKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMSDKLTFSVIWTLTPEGKILDEWFGRTIIRSCTKLSYEHAQSMIESPTEKIPAKELPPISPEHSSEEVHQQNADKDGAAHLQASHSPSAEDAEAQPSTEERLPETRGICDRDPDTRLFFLQQQSRVLEAKPQNTIRVEEQTTQLQI

Target ORF information:

RefSeq Version	NM_001257281.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257281.1

ORF Insert Sequence:

ATGAGCCATC CTGACTACAG AATGAACCTC CGGCCCCTGG GGACCCCCAG AGGTGTGTCT 
GCTGTGGCTG GTCCACATGA CATTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAGG 
TCCACACGAG GGAAGAAAAA GAGCATATTT GAAACTTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAGAGGAAC ACTCATCCAG GGTGTATTGA GAATTAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC CCCGGATGGT GATCGAGACA TTTTTATTGA TGGGGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGATCTG GTGGTCGTGA AACTGCTTCC CGAGGAGCAT 
TGGAAGGTAG TTAAACCAGA GAGCAATGAC AAAGAAACAG AAGCTGCGTA TGAATCAGAT 
ATCCCCGAGG AGCTCTGTGG ACACCATCTC CCGCAACAGT CCCTGAAAAG CTATAATGAC 
AGTCCTGATG TCATTGTAGA GGCTCAGTTT GATGGCAGCG ACTCAGAAGA TGGACATGGC 
ATCACACAAA ATGTGCTGGT TGATGGTGTT AAGAAACTCT CAGTTTGTGT TTCTGAGAAA 
GGAAGAGAGG ATGGTGATGC ACCGGTTACA AAAGATGAGA CCACCTGCAT TTCACAAGAC 
ACAAGAGCTT TATCGGAGAA ATCCCTGCAA AGATCAGCAA AGGTGGTTTA CATCTTGGAG 
AAAAAACATT CTCGAGCAGC AACCGGCTTC CTCAAACTCT TGGCTGATAA GAACAGCGAA 
CTGTTTAGGA AATACGCCCT GTTTTCTCCC TCAGACCACC GAGTGCCTAG AATTTATGTG 
CCTCTCAAGG ACTGTCCCCA GGACTTTGTG GCACGGCCTA AAGATTATGC CAACACACTG 
TTCATCTGCC GCATTGTGGA CTGGAAGGAG GACTGCAATT TTGCCCTGGG GCAGCTGGCT 
AAGAGTCTTG GGCAGGCTGG TGAAATTGAG CCTGAAACAG AAGGAATACT AACAGAGTAT 
GGCGTGGATT TCTCTGATTT CTCTTCAGAA GTTCTAGAAT GTCTTCCTCA AGGCCTGCCA 
TGGACAATTC CACCAGAGGA GTTCAGCAAG AGAAGGGATT TAAGAAAAGA CTGTATCTTC 
ACCATTGACC CATCAACCGC CCGAGACCTC GATGATGCCC TCTCCTGCAA GCCACTCGCT 
GACGGCAACT TCAAAGTGGG AGTTCACATT GCTGACGTGA GTTACTTTGT TCCGGAGGGA 
TCTGATCTGG ATAAAGTGGC TGCCGAGAGG GCTACAAGCG TCTACTTGGT TCAAAAGGTG 
GTCCCCATGC TTCCCAGGCT GCTGTGTGAG GAGCTGTGCA GCCTCAACCC CATGTCCGAC 
AAGCTGACCT TCTCTGTGAT CTGGACACTG ACTCCAGAGG GCAAGATCCT TGATGAATGG 
TTTGGCCGGA CCATCATCCG CTCCTGCACC AAACTTAGCT ACGAGCATGC ACAGAGCATG 
ATTGAAAGCC CAACTGAGAA AATCCCTGCG AAAGAGCTGC CCCCCATTTC CCCAGAGCAT 
AGCAGCGAGG AGGTACACCA GCAGAACGCA GACAAGGATG GGGCTGCCCA TCTTCAGGCC 
TCTCACAGCC CCTCTGCTGA AGATGCAGAA GCACAGCCCT CCACAGAGGA ACGCCTGCCT 
GAGACTCGGG GCATATGTGA CAGGGATCCA GACACACGAC TGTTTTTCCT TCAGCAACAG 
AGCCGTGTAT TGGAAGCAAA GCCCCAAAAC ACGATAAGAG TAGAGGAGCA GACAACCCAG 
TTGCAGATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIS3L2 ( NM_001257281.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244210.1 Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20305
Clone ID Related Accession (Same CDS sequence)	NM_001257281.1 , NM_001257281.2
Accession Version	NM_001257281.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	DIS3-like exonuclease 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK094293.1 and AC068134.5. On Jun 2, 2019 this sequence version replaced NM_001257281.1. Transcript Variant: This variant (2) lacks several exons and includes an alternate 3' terminal exon compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK094293.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAGCCATC CTGACTACAG AATGAACCTC CGGCCCCTGG GGACCCCCAG AGGTGTGTCT 
GCTGTGGCTG GTCCACATGA CATTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAGG 
TCCACACGAG GGAAGAAAAA GAGCATATTT GAAACTTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAGAGGAAC ACTCATCCAG GGTGTATTGA GAATTAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC CCCGGATGGT GATCGAGACA TTTTTATTGA TGGGGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGATCTG GTGGTCGTGA AACTGCTTCC CGAGGAGCAT 
TGGAAGGTAG TTAAACCAGA GAGCAATGAC AAAGAAACAG AAGCTGCGTA TGAATCAGAT 
ATCCCCGAGG AGCTCTGTGG ACACCATCTC CCGCAACAGT CCCTGAAAAG CTATAATGAC 
AGTCCTGATG TCATTGTAGA GGCTCAGTTT GATGGCAGCG ACTCAGAAGA TGGACATGGC 
ATCACACAAA ATGTGCTGGT TGATGGTGTT AAGAAACTCT CAGTTTGTGT TTCTGAGAAA 
GGAAGAGAGG ATGGTGATGC ACCGGTTACA AAAGATGAGA CCACCTGCAT TTCACAAGAC 
ACAAGAGCTT TATCGGAGAA ATCCCTGCAA AGATCAGCAA AGGTGGTTTA CATCTTGGAG 
AAAAAACATT CTCGAGCAGC AACCGGCTTC CTCAAACTCT TGGCTGATAA GAACAGCGAA 
CTGTTTAGGA AATACGCCCT GTTTTCTCCC TCAGACCACC GAGTGCCTAG AATTTATGTG 
CCTCTCAAGG ACTGTCCCCA GGACTTTGTG GCACGGCCTA AAGATTATGC CAACACACTG 
TTCATCTGCC GCATTGTGGA CTGGAAGGAG GACTGCAATT TTGCCCTGGG GCAGCTGGCT 
AAGAGTCTTG GGCAGGCTGG TGAAATTGAG CCTGAAACAG AAGGAATACT AACAGAGTAT 
GGCGTGGATT TCTCTGATTT CTCTTCAGAA GTTCTAGAAT GTCTTCCTCA AGGCCTGCCA 
TGGACAATTC CACCAGAGGA GTTCAGCAAG AGAAGGGATT TAAGAAAAGA CTGTATCTTC 
ACCATTGACC CATCAACCGC CCGAGACCTC GATGATGCCC TCTCCTGCAA GCCACTCGCT 
GACGGCAACT TCAAAGTGGG AGTTCACATT GCTGACGTGA GTTACTTTGT TCCGGAGGGA 
TCTGATCTGG ATAAAGTGGC TGCCGAGAGG GCTACAAGCG TCTACTTGGT TCAAAAGGTG 
GTCCCCATGC TTCCCAGGCT GCTGTGTGAG GAGCTGTGCA GCCTCAACCC CATGTCCGAC 
AAGCTGACCT TCTCTGTGAT CTGGACACTG ACTCCAGAGG GCAAGATCCT TGATGAATGG 
TTTGGCCGGA CCATCATCCG CTCCTGCACC AAACTTAGCT ACGAGCATGC ACAGAGCATG 
ATTGAAAGCC CAACTGAGAA AATCCCTGCG AAAGAGCTGC CCCCCATTTC CCCAGAGCAT 
AGCAGCGAGG AGGTACACCA GCAGAACGCA GACAAGGATG GGGCTGCCCA TCTTCAGGCC 
TCTCACAGCC CCTCTGCTGA AGATGCAGAA GCACAGCCCT CCACAGAGGA ACGCCTGCCT 
GAGACTCGGG GCATATGTGA CAGGGATCCA GACACACGAC TGTTTTTCCT TCAGCAACAG 
AGCCGTGTAT TGGAAGCAAA GCCCCAAAAC ACGATAAGAG TAGAGGAGCA GACAACCCAG 
TTGCAGATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244210.1
CDS	147..1958
Translation	MSHPDYRMNLRPLGTPRGVSAVAGPHDIGASPGDKKSKNRSTRGKKKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRALNGDLVVVKLLPEEHWKVVKPESNDKETEAAYESDIPEELCGHHLPQQSLKSYNDSPDVIVEAQFDGSDSEDGHGITQNVLVDGVKKLSVCVSEKGREDGDAPVTKDETTCISQDTRALSEKSLQRSAKVVYILEKKHSRAATGFLKLLADKNSELFRKYALFSPSDHRVPRIYVPLKDCPQDFVARPKDYANTLFICRIVDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQGLPWTIPPEEFSKRRDLRKDCIFTIDPSTARDLDDALSCKPLADGNFKVGVHIADVSYFVPEGSDLDKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMSDKLTFSVIWTLTPEGKILDEWFGRTIIRSCTKLSYEHAQSMIESPTEKIPAKELPPISPEHSSEEVHQQNADKDGAAHLQASHSPSAEDAEAQPSTEERLPETRGICDRDPDTRLFFLQQQSRVLEAKPQNTIRVEEQTTQLQI

Target ORF information:

RefSeq Version	NM_001257281.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257281.2

ORF Insert Sequence:

ATGAGCCATC CTGACTACAG AATGAACCTC CGGCCCCTGG GGACCCCCAG AGGTGTGTCT 
GCTGTGGCTG GTCCACATGA CATTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAGG 
TCCACACGAG GGAAGAAAAA GAGCATATTT GAAACTTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAGAGGAAC ACTCATCCAG GGTGTATTGA GAATTAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC CCCGGATGGT GATCGAGACA TTTTTATTGA TGGGGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGATCTG GTGGTCGTGA AACTGCTTCC CGAGGAGCAT 
TGGAAGGTAG TTAAACCAGA GAGCAATGAC AAAGAAACAG AAGCTGCGTA TGAATCAGAT 
ATCCCCGAGG AGCTCTGTGG ACACCATCTC CCGCAACAGT CCCTGAAAAG CTATAATGAC 
AGTCCTGATG TCATTGTAGA GGCTCAGTTT GATGGCAGCG ACTCAGAAGA TGGACATGGC 
ATCACACAAA ATGTGCTGGT TGATGGTGTT AAGAAACTCT CAGTTTGTGT TTCTGAGAAA 
GGAAGAGAGG ATGGTGATGC ACCGGTTACA AAAGATGAGA CCACCTGCAT TTCACAAGAC 
ACAAGAGCTT TATCGGAGAA ATCCCTGCAA AGATCAGCAA AGGTGGTTTA CATCTTGGAG 
AAAAAACATT CTCGAGCAGC AACCGGCTTC CTCAAACTCT TGGCTGATAA GAACAGCGAA 
CTGTTTAGGA AATACGCCCT GTTTTCTCCC TCAGACCACC GAGTGCCTAG AATTTATGTG 
CCTCTCAAGG ACTGTCCCCA GGACTTTGTG GCACGGCCTA AAGATTATGC CAACACACTG 
TTCATCTGCC GCATTGTGGA CTGGAAGGAG GACTGCAATT TTGCCCTGGG GCAGCTGGCT 
AAGAGTCTTG GGCAGGCTGG TGAAATTGAG CCTGAAACAG AAGGAATACT AACAGAGTAT 
GGCGTGGATT TCTCTGATTT CTCTTCAGAA GTTCTAGAAT GTCTTCCTCA AGGCCTGCCA 
TGGACAATTC CACCAGAGGA GTTCAGCAAG AGAAGGGATT TAAGAAAAGA CTGTATCTTC 
ACCATTGACC CATCAACCGC CCGAGACCTC GATGATGCCC TCTCCTGCAA GCCACTCGCT 
GACGGCAACT TCAAAGTGGG AGTTCACATT GCTGACGTGA GTTACTTTGT TCCGGAGGGA 
TCTGATCTGG ATAAAGTGGC TGCCGAGAGG GCTACAAGCG TCTACTTGGT TCAAAAGGTG 
GTCCCCATGC TTCCCAGGCT GCTGTGTGAG GAGCTGTGCA GCCTCAACCC CATGTCCGAC 
AAGCTGACCT TCTCTGTGAT CTGGACACTG ACTCCAGAGG GCAAGATCCT TGATGAATGG 
TTTGGCCGGA CCATCATCCG CTCCTGCACC AAACTTAGCT ACGAGCATGC ACAGAGCATG 
ATTGAAAGCC CAACTGAGAA AATCCCTGCG AAAGAGCTGC CCCCCATTTC CCCAGAGCAT 
AGCAGCGAGG AGGTACACCA GCAGAACGCA GACAAGGATG GGGCTGCCCA TCTTCAGGCC 
TCTCACAGCC CCTCTGCTGA AGATGCAGAA GCACAGCCCT CCACAGAGGA ACGCCTGCCT 
GAGACTCGGG GCATATGTGA CAGGGATCCA GACACACGAC TGTTTTTCCT TCAGCAACAG 
AGCCGTGTAT TGGAAGCAAA GCCCCAAAAC ACGATAAGAG TAGAGGAGCA GACAACCCAG 
TTGCAGATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIS3L2 ( NM_001257282.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244211.1 Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22619
Clone ID Related Accession (Same CDS sequence)	NM_001257282.1
Accession Version	NM_001257282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	DIS3-like exonuclease 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK094293.1, AF443854.1, AC019130.6 and AC138658.1. Transcript Variant: This variant (3) lacks several exons and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform 3, which is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF443854.1, SRR1803617.193869.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCATC CTGACTACAG AATGAACCTC CGGCCCCTGG GGACCCCCAG AGGTGTGTCT 
GCTGTGGCTG GTCCACATGA CATTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAGG 
TCCACACGAG GGAAGAAAAA GAGCATATTT GAAACTTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAGAGGAAC ACTCATCCAG GGTGTATTGA GAATTAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC CCCGGATGGT GATCGAGACA TTTTTATTGA TGGGGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGATCTG GTGGTCGTGA AACTGCTTCC CGAGGAGCAT 
TGGAAGGTAG TTAAACCAGA GAGCAATGAC AAAGAAACAG AAGCTGCGTA TGAATCAGAT 
ATCCCCGAGG AGCTCTGTGG ACACCATCTC CCGCAACAGT CCCTGAAAAG CTATAATGAC 
AGTCCTGATG TCATTGTAGA GGCTCAGTTT GATGGCAGCG ACTCAGAAGA TGGACATGGC 
ATCACACAAA ATGTGCTGGT TGATGGTGTT AAGAAACTCT CAGTTTGTGT TTCTGAGAAA 
GGAAGAGAGG ATGGTGATGC ACCGGTTACA AAAGATGAGA CCACCTGCAT TTCACAAGAC 
ACAAGAGCTT TATCGGAGAA ATCCCTGCAA AGATCAGCAA AGGTCATTGC CTACAGATTT 
TCTCCACGTG TCCAAATGGC TTTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244211.1
CDS	277..1026
Translation	MSHPDYRMNLRPLGTPRGVSAVAGPHDIGASPGDKKSKNRSTRGKKKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRALNGDLVVVKLLPEEHWKVVKPESNDKETEAAYESDIPEELCGHHLPQQSLKSYNDSPDVIVEAQFDGSDSEDGHGITQNVLVDGVKKLSVCVSEKGREDGDAPVTKDETTCISQDTRALSEKSLQRSAKVIAYRFSPRVQMAFT

Target ORF information:

RefSeq Version	NM_001257282.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DIS3 like 3'-5' exoribonuclease 2 (DIS3L2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257282.1

ORF Insert Sequence:

ATGAGCCATC CTGACTACAG AATGAACCTC CGGCCCCTGG GGACCCCCAG AGGTGTGTCT 
GCTGTGGCTG GTCCACATGA CATTGGTGCT TCGCCAGGTG ACAAAAAGTC AAAGAACAGG 
TCCACACGAG GGAAGAAAAA GAGCATATTT GAAACTTACA TGTCCAAGGA GGATGTTTCA 
GAAGGCTTGA AGAGAGGAAC ACTCATCCAG GGTGTATTGA GAATTAATCC AAAGAAGTTT 
CATGAAGCCT TCATTCCTTC CCCGGATGGT GATCGAGACA TTTTTATTGA TGGGGTTGTT 
GCTCGTAATA GAGCCTTAAA TGGGGATCTG GTGGTCGTGA AACTGCTTCC CGAGGAGCAT 
TGGAAGGTAG TTAAACCAGA GAGCAATGAC AAAGAAACAG AAGCTGCGTA TGAATCAGAT 
ATCCCCGAGG AGCTCTGTGG ACACCATCTC CCGCAACAGT CCCTGAAAAG CTATAATGAC 
AGTCCTGATG TCATTGTAGA GGCTCAGTTT GATGGCAGCG ACTCAGAAGA TGGACATGGC 
ATCACACAAA ATGTGCTGGT TGATGGTGTT AAGAAACTCT CAGTTTGTGT TTCTGAGAAA 
GGAAGAGAGG ATGGTGATGC ACCGGTTACA AAAGATGAGA CCACCTGCAT TTCACAAGAC 
ACAAGAGCTT TATCGGAGAA ATCCCTGCAA AGATCAGCAA AGGTCATTGC CTACAGATTT 
TCTCCACGTG TCCAAATGGC TTTCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Immunohistochemical localization of exoribonucleases (DIS3L2 and XRN1) in intranuclear inclusion body disease.
Neuroscience letters662389-394(2018 Jan)
Mori F,Tanji K,Miki Y,Toyoshima Y,Sasaki H,Yoshida M,Kakita A,Takahashi H,Wakabayashi K

Lin28a uses distinct mechanisms of binding to RNA and affects miRNA levels positively and negatively.
RNA (New York, N.Y.)23(3)317-332(2017 03)
Nowak JS,Hobor F,Downie Ruiz Velasco A,Choudhury NR,Heikel G,Kerr A,Ramos A,Michlewski G

Perlman syndrome nuclease DIS3L2 controls cytoplasmic non-coding RNAs and provides surveillance pathway for maturing snRNAs.
Nucleic acids research44(21)10437-10453(2016 Dec)
?abno A,Warkocki Z,Kuli?ski T,Krawczyk PS,Bijata K,Tomecki R,Dziembowski A

The roles of the exoribonucleases DIS3L2 and XRN1 in human disease.
Biochemical Society transactions44(5)1377-1384(2016 10)
Pashler AL,Towler BP,Jones CI,Newbury SF

TUT-DIS3L2 is a mammalian surveillance pathway for aberrant structured non-coding RNAs.
The EMBO journal35(20)2179-2191(2016 10)
Ustianenko D,Pasulka J,Feketova Z,Bednarik L,Zigackova D,Fortova A,Zavolan M,Vanacova S

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DIS3L2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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