COL8A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL8A1
Entrez Gene ID	1295
Full Name	collagen type VIII alpha 1 chain
Synonyms	C3orf7
General protein information	Preferred Names collagen type VIII alpha 1 chain Names collagen alpha-1(VIII) chain cell proliferation-inducing protein 41 collagen VIII, alpha-1 polypeptide collagen, type VIII, alpha 1 endothelial collagen smag-64 smooth muscle cell-expressed and macrophage conditioned medium-induced protein smag-64
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q12.1
Summary	This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Dec 2011].
Disorder MIM:	120251

mRNA and Protein(s)

mRNA	Protein	Name
NM_020351.3	NP_065084.2	collagen alpha-1(VIII) chain precursor
NM_001850.5	NP_001841.2	collagen alpha-1(VIII) chain precursor
NM_020351.4	NP_065084.2	collagen alpha-1(VIII) chain precursor
NM_001850.4	NP_001841.2	collagen alpha-1(VIII) chain precursor

Pathways from BioSystems

Homologies

	COL8A1	XP_002802671.1
Mus musculus (house mouse)	Col8a1	NP_031765.2
Gallus gallus (chicken)	COL8A1	XP_004938297.1
Bos taurus (cattle)	COL8A1	NP_001094646.1
Danio rerio (zebrafish)	col8a1b	XP_690914.3
Homo sapiens (human)	COL8A1	NP_001841.2
Pan troglodytes (chimpanzee)	COL8A1	XP_001142044.1
Rattus norvegicus (Norway rat)	Col8a1	NP_001100570.1
Xenopus tropicalis (tropical clawed frog)	col8a1	XP_002941034.1
Canis lupus familiaris (dog)	COL8A1	XP_545072.3

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V, Cutillas PR, Balkwill FR, Hynes RO
Journal of proteome research16(8)3083-3091(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High COL8A1 level is associated with chronic obstructive pulmonary disease and cancer.
Title: Type VIII collagen is elevated in diseases associated with angiogenesis and vascular remodeling.

High COL8A1 expression is associated with early loss of kidney function.
Title: Biomarkers for Refractory Lupus Nephritis: A Microarray Study of Kidney Tissue.

The COL8A1 rs13095226 polymorphism is not associated with nAMD or PCV, which suggesting this gene maybe not a susceptibility gene locus for neovascular age-related macular degeneration (nAMD) or polypoidal choroidal vasculopathy (PCV) in Chinese subjects.
Title: COL8A1 rs13095226 polymorphism shows no association with neovascular age-related macular degeneration or polypoidal choroidal vasculopathy in Chinese subjects.

Data indicate that complement factor H (CFH) R1210C and common variants in COL8A1 and RAD51B plus six genes contribute predictive information for advanced macular degeneration (AMD) beyond macular and behavioral phenotypes.
Title: Three new genetic loci (R1210C in CFH, variants in COL8A1 and RAD51B) are independently related to progression to advanced macular degeneration.

The results are consistent with the proposal that rare CNVs play a role in TS aetiology and suggest a possible role for rearrangements in the COL8A1 and NRXN1 gene regions.
Title: CNV analysis in Tourette syndrome implicates large genomic rearrangements in COL8A1 and NRXN1.

The following COL8A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL8A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28226	NM_020351.4 Latest version!	Homo sapiens collagen type VIII alpha 1 chain (COL8A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28226	NM_001850.5 Latest version!	Homo sapiens collagen type VIII alpha 1 chain (COL8A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu28226	NM_020351.3	Homo sapiens collagen type VIII alpha 1 chain (COL8A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28226	NM_001850.4	Homo sapiens collagen type VIII alpha 1 chain (COL8A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL8A1 ( NM_020351.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065084.2 Homo sapiens collagen type VIII alpha 1 chain (COL8A1), transcript variant 2, mRNA.

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CloneID	OHu28226
Clone ID Related Accession (Same CDS sequence)	NM_020351.3 , NM_001850.5 , NM_020351.4 , NM_001850.4
Accession Version	NM_020351.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	collagen alpha-1(VIII) chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK311449.1, CN484310.1, BT009917.1, BG938623.1 and DA663372.1. On Dec 31, 2011 this sequence version replaced NM_020351.2. Transcript Variant: This variant (2) lacks an exon in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.181374.1, SRR1803613.185753.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGC TGCCTGGCCC TCTGCAGCTG CTGGGAGTGC TGCTTACCAT TTCCCTGAGT 
TCCATCAGGC TCATTCAGGC TGGTGCCTAC TATGGGATCA AGCCGCTGCC ACCTCAAATT 
CCTCCTCAGA TGCCACCACA AATTCCACAA TACCAGCCCC TGGGTCAGCA AGTACCTCAC 
ATGCCTTTGG CCAAAGATGG CCTTGCCATG GGCAAGGAGA TGCCCCACTT GCAGTATGGC 
AAAGAGTATC CACACCTACC CCAATATATG AAGGAAATTC AACCGGCGCC AAGAATGGGC 
AAGGAAGCCG TACCCAAGAA AGGCAAAGAA ATACCATTAG CCAGTTTACG AGGGGAACAA 
GGTCCCCGTG GAGAGCCTGG CCCAAGAGGA CCACCTGGGC CCCCTGGTTT GCCAGGTCAT 
GGGATACCTG GAATTAAAGG AAAACCAGGG CCACAGGGAT ATCCAGGAGT TGGAAAGCCA 
GGTATGCCTG GAATGCCAGG GAAGCCAGGA GCCATGGGCA TGCCTGGGGC AAAAGGAGAA 
ATTGGACAGA AAGGGGAAAT TGGGCCTATG GGGATCCCAG GACCACAAGG ACCTCCAGGG 
CCTCATGGAC TTCCTGGCAT TGGGAAGCCA GGTGGGCCAG GGTTACCAGG GCAACCAGGA 
CCAAAGGGTG ATCGAGGACC CAAAGGACTA CCAGGACCTC AAGGCCTTCG GGGTCCTAAA 
GGAGACAAGG GCTTCGGGAT GCCAGGTGCG CCAGGTGTAA AGGGGCCTCC AGGGATGCAC 
GGCCCTCCCG GCCCTGTTGG ACTGCCAGGA GTGGGCAAAC CAGGAGTGAC AGGCTTCCCT 
GGGCCCCAGG GCCCCCTGGG AAAGCCAGGG GCTCCAGGAG AACCTGGGCC ACAAGGCCCT 
ATTGGGGTAC CGGGGGTTCA AGGACCTCCT GGGATACCCG GAATTGGAAA GCCAGGCCAG 
GATGGGATCC CAGGCCAGCC AGGATTTCCA GGTGGCAAAG GGGAGCAAGG ACTGCCAGGG 
CTACCAGGAC CCCCAGGCCT TCCAGGGATT GGGAAACCAG GCTTCCCAGG ACCCAAAGGT 
GACCGGGGCA TGGGAGGTGT TCCTGGGGCT CTTGGACCAA GAGGGGAGAA AGGACCAATA 
GGTGCCCCAG GAATAGGGGG TCCTCCAGGA GAGCCAGGCC TGCCTGGAAT CCCAGGTCCT 
ATGGGCCCTC CAGGTGCTAT TGGTTTTCCT GGACCCAAAG GAGAAGGTGG GATTGTAGGG 
CCACAGGGGC CACCAGGTCC CAAGGGTGAG CCAGGGCTTC AAGGCTTCCC AGGAAAGCCA 
GGTTTCCTTG GTGAAGTAGG GCCTCCTGGC ATGAGGGGTT TGCCAGGTCC CATAGGGCCC 
AAGGGGGAAG CTGGGCAAAA AGGTGTACCA GGACTCCCTG GTGTTCCAGG GCTTCTCGGA 
CCTAAGGGAG AGCCAGGAAT CCCAGGGGAT CAGGGTTTAC AGGGCCCCCC AGGTATCCCA 
GGGATTGGGG GCCCTAGTGG CCCCATTGGA CCACCTGGGA TTCCAGGCCC CAAAGGGGAG 
CCGGGCCTCC CAGGGCCCCC TGGGTTCCCT GGTATAGGGA AACCCGGAGT GGCAGGACTT 
CATGGCCCCC CAGGGAAGCC TGGTGCCCTT GGTCCTCAAG GCCAGCCTGG CCTTCCAGGA 
CCCCCAGGCC CTCCAGGACC TCCAGGACCC CCAGCTGTGA TGCCCCCTAC ACCACCACCC 
CAGGGAGAGT ATCTGCCAGA TATGGGGCTG GGAATTGATG GCGTGAAACC CCCCCATGCC 
TACGGGGCTA AGAAAGGCAA GAATGGAGGG CCAGCCTATG AGATGCCTGC ATTTACCGCC 
GAGCTAACCG CACCTTTCCC ACCGGTGGGG GCCCCAGTGA AGTTTAACAA ACTGCTGTAT 
AACGGCAGAC AGAACTACAA CCCGCAGACA GGCATCTTCA CCTGTGAGGT CCCTGGTGTC 
TACTACTTTG CATACCACGT TCACTGCAAG GGGGGCAACG TGTGGGTTGC TCTATTCAAG 
AACAACGAGC CCGTGATGTA CACGTACGAC GAGTACAAAA AGGGCTTCCT GGACCAGGCA 
TCTGGGAGTG CAGTGCTGCT GCTCAGGCCC GGAGACCGGG TGTTCCTCCA GATGCCCTCA 
GAACAGGCTG CAGGACTGTA TGCCGGGCAG TATGTCCACT CCTCCTTTTC AGGATATTTA 
TTGTATCCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065084.2
CDS	198..2432
Translation	MAVLPGPLQLLGVLLTISLSSIRLIQAGAYYGIKPLPPQIPPQMPPQIPQYQPLGQQVPHMPLAKDGLAMGKEMPHLQYGKEYPHLPQYMKEIQPAPRMGKEAVPKKGKEIPLASLRGEQGPRGEPGPRGPPGPPGLPGHGIPGIKGKPGPQGYPGVGKPGMPGMPGKPGAMGMPGAKGEIGQKGEIGPMGIPGPQGPPGPHGLPGIGKPGGPGLPGQPGPKGDRGPKGLPGPQGLRGPKGDKGFGMPGAPGVKGPPGMHGPPGPVGLPGVGKPGVTGFPGPQGPLGKPGAPGEPGPQGPIGVPGVQGPPGIPGIGKPGQDGIPGQPGFPGGKGEQGLPGLPGPPGLPGIGKPGFPGPKGDRGMGGVPGALGPRGEKGPIGAPGIGGPPGEPGLPGIPGPMGPPGAIGFPGPKGEGGIVGPQGPPGPKGEPGLQGFPGKPGFLGEVGPPGMRGLPGPIGPKGEAGQKGVPGLPGVPGLLGPKGEPGIPGDQGLQGPPGIPGIGGPSGPIGPPGIPGPKGEPGLPGPPGFPGIGKPGVAGLHGPPGKPGALGPQGQPGLPGPPGPPGPPGPPAVMPPTPPPQGEYLPDMGLGIDGVKPPHAYGAKKGKNGGPAYEMPAFTAELTAPFPPVGAPVKFNKLLYNGRQNYNPQTGIFTCEVPGVYYFAYHVHCKGGNVWVALFKNNEPVMYTYDEYKKGFLDQASGSAVLLLRPGDRVFLQMPSEQAAGLYAGQYVHSSFSGYLLYPM

Target ORF information:

RefSeq Version	NM_020351.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VIII alpha 1 chain (COL8A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020351.3

ORF Insert Sequence:

ATGGCTGTGC TGCCTGGCCC TCTGCAGCTG CTGGGAGTGC TGCTTACCAT TTCCCTGAGT 
TCCATCAGGC TCATTCAGGC TGGTGCCTAC TATGGGATCA AGCCGCTGCC ACCTCAAATT 
CCTCCTCAGA TGCCACCACA AATTCCACAA TACCAGCCCC TGGGTCAGCA AGTACCTCAC 
ATGCCTTTGG CCAAAGATGG CCTTGCCATG GGCAAGGAGA TGCCCCACTT GCAGTATGGC 
AAAGAGTATC CACACCTACC CCAATATATG AAGGAAATTC AACCGGCGCC AAGAATGGGC 
AAGGAAGCCG TACCCAAGAA AGGCAAAGAA ATACCATTAG CCAGTTTACG AGGGGAACAA 
GGTCCCCGTG GAGAGCCTGG CCCAAGAGGA CCACCTGGGC CCCCTGGTTT GCCAGGTCAT 
GGGATACCTG GAATTAAAGG AAAACCAGGG CCACAGGGAT ATCCAGGAGT TGGAAAGCCA 
GGTATGCCTG GAATGCCAGG GAAGCCAGGA GCCATGGGCA TGCCTGGGGC AAAAGGAGAA 
ATTGGACAGA AAGGGGAAAT TGGGCCTATG GGGATCCCAG GACCACAAGG ACCTCCAGGG 
CCTCATGGAC TTCCTGGCAT TGGGAAGCCA GGTGGGCCAG GGTTACCAGG GCAACCAGGA 
CCAAAGGGTG ATCGAGGACC CAAAGGACTA CCAGGACCTC AAGGCCTTCG GGGTCCTAAA 
GGAGACAAGG GCTTCGGGAT GCCAGGTGCG CCAGGTGTAA AGGGGCCTCC AGGGATGCAC 
GGCCCTCCCG GCCCTGTTGG ACTGCCAGGA GTGGGCAAAC CAGGAGTGAC AGGCTTCCCT 
GGGCCCCAGG GCCCCCTGGG AAAGCCAGGG GCTCCAGGAG AACCTGGGCC ACAAGGCCCT 
ATTGGGGTAC CGGGGGTTCA AGGACCTCCT GGGATACCCG GAATTGGAAA GCCAGGCCAG 
GATGGGATCC CAGGCCAGCC AGGATTTCCA GGTGGCAAAG GGGAGCAAGG ACTGCCAGGG 
CTACCAGGAC CCCCAGGCCT TCCAGGGATT GGGAAACCAG GCTTCCCAGG ACCCAAAGGT 
GACCGGGGCA TGGGAGGTGT TCCTGGGGCT CTTGGACCAA GAGGGGAGAA AGGACCAATA 
GGTGCCCCAG GAATAGGGGG TCCTCCAGGA GAGCCAGGCC TGCCTGGAAT CCCAGGTCCT 
ATGGGCCCTC CAGGTGCTAT TGGTTTTCCT GGACCCAAAG GAGAAGGTGG GATTGTAGGG 
CCACAGGGGC CACCAGGTCC CAAGGGTGAG CCAGGGCTTC AAGGCTTCCC AGGAAAGCCA 
GGTTTCCTTG GTGAAGTAGG GCCTCCTGGC ATGAGGGGTT TGCCAGGTCC CATAGGGCCC 
AAGGGGGAAG CTGGGCAAAA AGGTGTACCA GGACTCCCTG GTGTTCCAGG GCTTCTCGGA 
CCTAAGGGAG AGCCAGGAAT CCCAGGGGAT CAGGGTTTAC AGGGCCCCCC AGGTATCCCA 
GGGATTGGGG GCCCTAGTGG CCCCATTGGA CCACCTGGGA TTCCAGGCCC CAAAGGGGAG 
CCGGGCCTCC CAGGGCCCCC TGGGTTCCCT GGTATAGGGA AACCCGGAGT GGCAGGACTT 
CATGGCCCCC CAGGGAAGCC TGGTGCCCTT GGTCCTCAAG GCCAGCCTGG CCTTCCAGGA 
CCCCCAGGCC CTCCAGGACC TCCAGGACCC CCAGCTGTGA TGCCCCCTAC ACCACCACCC 
CAGGGAGAGT ATCTGCCAGA TATGGGGCTG GGAATTGATG GCGTGAAACC CCCCCATGCC 
TACGGGGCTA AGAAAGGCAA GAATGGAGGG CCAGCCTATG AGATGCCTGC ATTTACCGCC 
GAGCTAACCG CACCTTTCCC ACCGGTGGGG GCCCCAGTGA AGTTTAACAA ACTGCTGTAT 
AACGGCAGAC AGAACTACAA CCCGCAGACA GGCATCTTCA CCTGTGAGGT CCCTGGTGTC 
TACTACTTTG CATACCACGT TCACTGCAAG GGGGGCAACG TGTGGGTTGC TCTATTCAAG 
AACAACGAGC CCGTGATGTA CACGTACGAC GAGTACAAAA AGGGCTTCCT GGACCAGGCA 
TCTGGGAGTG CAGTGCTGCT GCTCAGGCCC GGAGACCGGG TGTTCCTCCA GATGCCCTCA 
GAACAGGCTG CAGGACTGTA TGCCGGGCAG TATGTCCACT CCTCCTTTTC AGGATATTTA 
TTGTATCCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL8A1 ( NM_001850.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001841.2 Homo sapiens collagen type VIII alpha 1 chain (COL8A1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28226
Clone ID Related Accession (Same CDS sequence)	NM_020351.3 , NM_001850.5 , NM_020351.4 , NM_001850.4
Accession Version	NM_001850.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	collagen alpha-1(VIII) chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055723.22, AK311449.1, BC013581.1, CB269463.1, DA663372.1 and AC069222.23. On May 31, 2019 this sequence version replaced NM_001850.4. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013581.1, SRR1803613.147377.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGC TGCCTGGCCC TCTGCAGCTG CTGGGAGTGC TGCTTACCAT TTCCCTGAGT 
TCCATCAGGC TCATTCAGGC TGGTGCCTAC TATGGGATCA AGCCGCTGCC ACCTCAAATT 
CCTCCTCAGA TGCCACCACA AATTCCACAA TACCAGCCCC TGGGTCAGCA AGTACCTCAC 
ATGCCTTTGG CCAAAGATGG CCTTGCCATG GGCAAGGAGA TGCCCCACTT GCAGTATGGC 
AAAGAGTATC CACACCTACC CCAATATATG AAGGAAATTC AACCGGCGCC AAGAATGGGC 
AAGGAAGCCG TACCCAAGAA AGGCAAAGAA ATACCATTAG CCAGTTTACG AGGGGAACAA 
GGTCCCCGTG GAGAGCCTGG CCCAAGAGGA CCACCTGGGC CCCCTGGTTT GCCAGGTCAT 
GGGATACCTG GAATTAAAGG AAAACCAGGG CCACAGGGAT ATCCAGGAGT TGGAAAGCCA 
GGTATGCCTG GAATGCCAGG GAAGCCAGGA GCCATGGGCA TGCCTGGGGC AAAAGGAGAA 
ATTGGACAGA AAGGGGAAAT TGGGCCTATG GGGATCCCAG GACCACAAGG ACCTCCAGGG 
CCTCATGGAC TTCCTGGCAT TGGGAAGCCA GGTGGGCCAG GGTTACCAGG GCAACCAGGA 
CCAAAGGGTG ATCGAGGACC CAAAGGACTA CCAGGACCTC AAGGCCTTCG GGGTCCTAAA 
GGAGACAAGG GCTTCGGGAT GCCAGGTGCG CCAGGTGTAA AGGGGCCTCC AGGGATGCAC 
GGCCCTCCCG GCCCTGTTGG ACTGCCAGGA GTGGGCAAAC CAGGAGTGAC AGGCTTCCCT 
GGGCCCCAGG GCCCCCTGGG AAAGCCAGGG GCTCCAGGAG AACCTGGGCC ACAAGGCCCT 
ATTGGGGTAC CGGGGGTTCA AGGACCTCCT GGGATACCCG GAATTGGAAA GCCAGGCCAG 
GATGGGATCC CAGGCCAGCC AGGATTTCCA GGTGGCAAAG GGGAGCAAGG ACTGCCAGGG 
CTACCAGGAC CCCCAGGCCT TCCAGGGATT GGGAAACCAG GCTTCCCAGG ACCCAAAGGT 
GACCGGGGCA TGGGAGGTGT TCCTGGGGCT CTTGGACCAA GAGGGGAGAA AGGACCAATA 
GGTGCCCCAG GAATAGGGGG TCCTCCAGGA GAGCCAGGCC TGCCTGGAAT CCCAGGTCCT 
ATGGGCCCTC CAGGTGCTAT TGGTTTTCCT GGACCCAAAG GAGAAGGTGG GATTGTAGGG 
CCACAGGGGC CACCAGGTCC CAAGGGTGAG CCAGGGCTTC AAGGCTTCCC AGGAAAGCCA 
GGTTTCCTTG GTGAAGTAGG GCCTCCTGGC ATGAGGGGTT TGCCAGGTCC CATAGGGCCC 
AAGGGGGAAG CTGGGCAAAA AGGTGTACCA GGACTCCCTG GTGTTCCAGG GCTTCTCGGA 
CCTAAGGGAG AGCCAGGAAT CCCAGGGGAT CAGGGTTTAC AGGGCCCCCC AGGTATCCCA 
GGGATTGGGG GCCCTAGTGG CCCCATTGGA CCACCTGGGA TTCCAGGCCC CAAAGGGGAG 
CCGGGCCTCC CAGGGCCCCC TGGGTTCCCT GGTATAGGGA AACCCGGAGT GGCAGGACTT 
CATGGCCCCC CAGGGAAGCC TGGTGCCCTT GGTCCTCAAG GCCAGCCTGG CCTTCCAGGA 
CCCCCAGGCC CTCCAGGACC TCCAGGACCC CCAGCTGTGA TGCCCCCTAC ACCACCACCC 
CAGGGAGAGT ATCTGCCAGA TATGGGGCTG GGAATTGATG GCGTGAAACC CCCCCATGCC 
TACGGGGCTA AGAAAGGCAA GAATGGAGGG CCAGCCTATG AGATGCCTGC ATTTACCGCC 
GAGCTAACCG CACCTTTCCC ACCGGTGGGG GCCCCAGTGA AGTTTAACAA ACTGCTGTAT 
AACGGCAGAC AGAACTACAA CCCGCAGACA GGCATCTTCA CCTGTGAGGT CCCTGGTGTC 
TACTACTTTG CATACCACGT TCACTGCAAG GGGGGCAACG TGTGGGTTGC TCTATTCAAG 
AACAACGAGC CCGTGATGTA CACGTACGAC GAGTACAAAA AGGGCTTCCT GGACCAGGCA 
TCTGGGAGTG CAGTGCTGCT GCTCAGGCCC GGAGACCGGG TGTTCCTCCA GATGCCCTCA 
GAACAGGCTG CAGGACTGTA TGCCGGGCAG TATGTCCACT CCTCCTTTTC AGGATATTTA 
TTGTATCCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001841.2
CDS	262..2496
Translation	MAVLPGPLQLLGVLLTISLSSIRLIQAGAYYGIKPLPPQIPPQMPPQIPQYQPLGQQVPHMPLAKDGLAMGKEMPHLQYGKEYPHLPQYMKEIQPAPRMGKEAVPKKGKEIPLASLRGEQGPRGEPGPRGPPGPPGLPGHGIPGIKGKPGPQGYPGVGKPGMPGMPGKPGAMGMPGAKGEIGQKGEIGPMGIPGPQGPPGPHGLPGIGKPGGPGLPGQPGPKGDRGPKGLPGPQGLRGPKGDKGFGMPGAPGVKGPPGMHGPPGPVGLPGVGKPGVTGFPGPQGPLGKPGAPGEPGPQGPIGVPGVQGPPGIPGIGKPGQDGIPGQPGFPGGKGEQGLPGLPGPPGLPGIGKPGFPGPKGDRGMGGVPGALGPRGEKGPIGAPGIGGPPGEPGLPGIPGPMGPPGAIGFPGPKGEGGIVGPQGPPGPKGEPGLQGFPGKPGFLGEVGPPGMRGLPGPIGPKGEAGQKGVPGLPGVPGLLGPKGEPGIPGDQGLQGPPGIPGIGGPSGPIGPPGIPGPKGEPGLPGPPGFPGIGKPGVAGLHGPPGKPGALGPQGQPGLPGPPGPPGPPGPPAVMPPTPPPQGEYLPDMGLGIDGVKPPHAYGAKKGKNGGPAYEMPAFTAELTAPFPPVGAPVKFNKLLYNGRQNYNPQTGIFTCEVPGVYYFAYHVHCKGGNVWVALFKNNEPVMYTYDEYKKGFLDQASGSAVLLLRPGDRVFLQMPSEQAAGLYAGQYVHSSFSGYLLYPM

Target ORF information:

RefSeq Version	NM_001850.5
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VIII alpha 1 chain (COL8A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001850.5

ORF Insert Sequence:

ATGGCTGTGC TGCCTGGCCC TCTGCAGCTG CTGGGAGTGC TGCTTACCAT TTCCCTGAGT 
TCCATCAGGC TCATTCAGGC TGGTGCCTAC TATGGGATCA AGCCGCTGCC ACCTCAAATT 
CCTCCTCAGA TGCCACCACA AATTCCACAA TACCAGCCCC TGGGTCAGCA AGTACCTCAC 
ATGCCTTTGG CCAAAGATGG CCTTGCCATG GGCAAGGAGA TGCCCCACTT GCAGTATGGC 
AAAGAGTATC CACACCTACC CCAATATATG AAGGAAATTC AACCGGCGCC AAGAATGGGC 
AAGGAAGCCG TACCCAAGAA AGGCAAAGAA ATACCATTAG CCAGTTTACG AGGGGAACAA 
GGTCCCCGTG GAGAGCCTGG CCCAAGAGGA CCACCTGGGC CCCCTGGTTT GCCAGGTCAT 
GGGATACCTG GAATTAAAGG AAAACCAGGG CCACAGGGAT ATCCAGGAGT TGGAAAGCCA 
GGTATGCCTG GAATGCCAGG GAAGCCAGGA GCCATGGGCA TGCCTGGGGC AAAAGGAGAA 
ATTGGACAGA AAGGGGAAAT TGGGCCTATG GGGATCCCAG GACCACAAGG ACCTCCAGGG 
CCTCATGGAC TTCCTGGCAT TGGGAAGCCA GGTGGGCCAG GGTTACCAGG GCAACCAGGA 
CCAAAGGGTG ATCGAGGACC CAAAGGACTA CCAGGACCTC AAGGCCTTCG GGGTCCTAAA 
GGAGACAAGG GCTTCGGGAT GCCAGGTGCG CCAGGTGTAA AGGGGCCTCC AGGGATGCAC 
GGCCCTCCCG GCCCTGTTGG ACTGCCAGGA GTGGGCAAAC CAGGAGTGAC AGGCTTCCCT 
GGGCCCCAGG GCCCCCTGGG AAAGCCAGGG GCTCCAGGAG AACCTGGGCC ACAAGGCCCT 
ATTGGGGTAC CGGGGGTTCA AGGACCTCCT GGGATACCCG GAATTGGAAA GCCAGGCCAG 
GATGGGATCC CAGGCCAGCC AGGATTTCCA GGTGGCAAAG GGGAGCAAGG ACTGCCAGGG 
CTACCAGGAC CCCCAGGCCT TCCAGGGATT GGGAAACCAG GCTTCCCAGG ACCCAAAGGT 
GACCGGGGCA TGGGAGGTGT TCCTGGGGCT CTTGGACCAA GAGGGGAGAA AGGACCAATA 
GGTGCCCCAG GAATAGGGGG TCCTCCAGGA GAGCCAGGCC TGCCTGGAAT CCCAGGTCCT 
ATGGGCCCTC CAGGTGCTAT TGGTTTTCCT GGACCCAAAG GAGAAGGTGG GATTGTAGGG 
CCACAGGGGC CACCAGGTCC CAAGGGTGAG CCAGGGCTTC AAGGCTTCCC AGGAAAGCCA 
GGTTTCCTTG GTGAAGTAGG GCCTCCTGGC ATGAGGGGTT TGCCAGGTCC CATAGGGCCC 
AAGGGGGAAG CTGGGCAAAA AGGTGTACCA GGACTCCCTG GTGTTCCAGG GCTTCTCGGA 
CCTAAGGGAG AGCCAGGAAT CCCAGGGGAT CAGGGTTTAC AGGGCCCCCC AGGTATCCCA 
GGGATTGGGG GCCCTAGTGG CCCCATTGGA CCACCTGGGA TTCCAGGCCC CAAAGGGGAG 
CCGGGCCTCC CAGGGCCCCC TGGGTTCCCT GGTATAGGGA AACCCGGAGT GGCAGGACTT 
CATGGCCCCC CAGGGAAGCC TGGTGCCCTT GGTCCTCAAG GCCAGCCTGG CCTTCCAGGA 
CCCCCAGGCC CTCCAGGACC TCCAGGACCC CCAGCTGTGA TGCCCCCTAC ACCACCACCC 
CAGGGAGAGT ATCTGCCAGA TATGGGGCTG GGAATTGATG GCGTGAAACC CCCCCATGCC 
TACGGGGCTA AGAAAGGCAA GAATGGAGGG CCAGCCTATG AGATGCCTGC ATTTACCGCC 
GAGCTAACCG CACCTTTCCC ACCGGTGGGG GCCCCAGTGA AGTTTAACAA ACTGCTGTAT 
AACGGCAGAC AGAACTACAA CCCGCAGACA GGCATCTTCA CCTGTGAGGT CCCTGGTGTC 
TACTACTTTG CATACCACGT TCACTGCAAG GGGGGCAACG TGTGGGTTGC TCTATTCAAG 
AACAACGAGC CCGTGATGTA CACGTACGAC GAGTACAAAA AGGGCTTCCT GGACCAGGCA 
TCTGGGAGTG CAGTGCTGCT GCTCAGGCCC GGAGACCGGG TGTTCCTCCA GATGCCCTCA 
GAACAGGCTG CAGGACTGTA TGCCGGGCAG TATGTCCACT CCTCCTTTTC AGGATATTTA 
TTGTATCCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL8A1 ( NM_020351.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065084.2 Homo sapiens collagen type VIII alpha 1 chain (COL8A1), transcript variant 2, mRNA.

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CloneID	OHu28226
Clone ID Related Accession (Same CDS sequence)	NM_020351.3 , NM_001850.5 , NM_020351.4 , NM_001850.4
Accession Version	NM_020351.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	collagen alpha-1(VIII) chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055723.22, AK311449.1, CN484310.1, BT009917.1, BG938623.1, DA663372.1 and AC069222.23. On Nov 22, 2018 this sequence version replaced NM_020351.3. Transcript Variant: This variant (2) lacks an exon in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.185753.1, SRR1803616.181374.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000652472.1/ ENSP00000498483.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGTGC TGCCTGGCCC TCTGCAGCTG CTGGGAGTGC TGCTTACCAT TTCCCTGAGT 
TCCATCAGGC TCATTCAGGC TGGTGCCTAC TATGGGATCA AGCCGCTGCC ACCTCAAATT 
CCTCCTCAGA TGCCACCACA AATTCCACAA TACCAGCCCC TGGGTCAGCA AGTACCTCAC 
ATGCCTTTGG CCAAAGATGG CCTTGCCATG GGCAAGGAGA TGCCCCACTT GCAGTATGGC 
AAAGAGTATC CACACCTACC CCAATATATG AAGGAAATTC AACCGGCGCC AAGAATGGGC 
AAGGAAGCCG TACCCAAGAA AGGCAAAGAA ATACCATTAG CCAGTTTACG AGGGGAACAA 
GGTCCCCGTG GAGAGCCTGG CCCAAGAGGA CCACCTGGGC CCCCTGGTTT GCCAGGTCAT 
GGGATACCTG GAATTAAAGG AAAACCAGGG CCACAGGGAT ATCCAGGAGT TGGAAAGCCA 
GGTATGCCTG GAATGCCAGG GAAGCCAGGA GCCATGGGCA TGCCTGGGGC AAAAGGAGAA 
ATTGGACAGA AAGGGGAAAT TGGGCCTATG GGGATCCCAG GACCACAAGG ACCTCCAGGG 
CCTCATGGAC TTCCTGGCAT TGGGAAGCCA GGTGGGCCAG GGTTACCAGG GCAACCAGGA 
CCAAAGGGTG ATCGAGGACC CAAAGGACTA CCAGGACCTC AAGGCCTTCG GGGTCCTAAA 
GGAGACAAGG GCTTCGGGAT GCCAGGTGCG CCAGGTGTAA AGGGGCCTCC AGGGATGCAC 
GGCCCTCCCG GCCCTGTTGG ACTGCCAGGA GTGGGCAAAC CAGGAGTGAC AGGCTTCCCT 
GGGCCCCAGG GCCCCCTGGG AAAGCCAGGG GCTCCAGGAG AACCTGGGCC ACAAGGCCCT 
ATTGGGGTAC CGGGGGTTCA AGGACCTCCT GGGATACCCG GAATTGGAAA GCCAGGCCAG 
GATGGGATCC CAGGCCAGCC AGGATTTCCA GGTGGCAAAG GGGAGCAAGG ACTGCCAGGG 
CTACCAGGAC CCCCAGGCCT TCCAGGGATT GGGAAACCAG GCTTCCCAGG ACCCAAAGGT 
GACCGGGGCA TGGGAGGTGT TCCTGGGGCT CTTGGACCAA GAGGGGAGAA AGGACCAATA 
GGTGCCCCAG GAATAGGGGG TCCTCCAGGA GAGCCAGGCC TGCCTGGAAT CCCAGGTCCT 
ATGGGCCCTC CAGGTGCTAT TGGTTTTCCT GGACCCAAAG GAGAAGGTGG GATTGTAGGG 
CCACAGGGGC CACCAGGTCC CAAGGGTGAG CCAGGGCTTC AAGGCTTCCC AGGAAAGCCA 
GGTTTCCTTG GTGAAGTAGG GCCTCCTGGC ATGAGGGGTT TGCCAGGTCC CATAGGGCCC 
AAGGGGGAAG CTGGGCAAAA AGGTGTACCA GGACTCCCTG GTGTTCCAGG GCTTCTCGGA 
CCTAAGGGAG AGCCAGGAAT CCCAGGGGAT CAGGGTTTAC AGGGCCCCCC AGGTATCCCA 
GGGATTGGGG GCCCTAGTGG CCCCATTGGA CCACCTGGGA TTCCAGGCCC CAAAGGGGAG 
CCGGGCCTCC CAGGGCCCCC TGGGTTCCCT GGTATAGGGA AACCCGGAGT GGCAGGACTT 
CATGGCCCCC CAGGGAAGCC TGGTGCCCTT GGTCCTCAAG GCCAGCCTGG CCTTCCAGGA 
CCCCCAGGCC CTCCAGGACC TCCAGGACCC CCAGCTGTGA TGCCCCCTAC ACCACCACCC 
CAGGGAGAGT ATCTGCCAGA TATGGGGCTG GGAATTGATG GCGTGAAACC CCCCCATGCC 
TACGGGGCTA AGAAAGGCAA GAATGGAGGG CCAGCCTATG AGATGCCTGC ATTTACCGCC 
GAGCTAACCG CACCTTTCCC ACCGGTGGGG GCCCCAGTGA AGTTTAACAA ACTGCTGTAT 
AACGGCAGAC AGAACTACAA CCCGCAGACA GGCATCTTCA CCTGTGAGGT CCCTGGTGTC 
TACTACTTTG CATACCACGT TCACTGCAAG GGGGGCAACG TGTGGGTTGC TCTATTCAAG 
AACAACGAGC CCGTGATGTA CACGTACGAC GAGTACAAAA AGGGCTTCCT GGACCAGGCA 
TCTGGGAGTG CAGTGCTGCT GCTCAGGCCC GGAGACCGGG TGTTCCTCCA GATGCCCTCA 
GAACAGGCTG CAGGACTGTA TGCCGGGCAG TATGTCCACT CCTCCTTTTC AGGATATTTA 
TTGTATCCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065084.2
CDS	200..2434
Translation	MAVLPGPLQLLGVLLTISLSSIRLIQAGAYYGIKPLPPQIPPQMPPQIPQYQPLGQQVPHMPLAKDGLAMGKEMPHLQYGKEYPHLPQYMKEIQPAPRMGKEAVPKKGKEIPLASLRGEQGPRGEPGPRGPPGPPGLPGHGIPGIKGKPGPQGYPGVGKPGMPGMPGKPGAMGMPGAKGEIGQKGEIGPMGIPGPQGPPGPHGLPGIGKPGGPGLPGQPGPKGDRGPKGLPGPQGLRGPKGDKGFGMPGAPGVKGPPGMHGPPGPVGLPGVGKPGVTGFPGPQGPLGKPGAPGEPGPQGPIGVPGVQGPPGIPGIGKPGQDGIPGQPGFPGGKGEQGLPGLPGPPGLPGIGKPGFPGPKGDRGMGGVPGALGPRGEKGPIGAPGIGGPPGEPGLPGIPGPMGPPGAIGFPGPKGEGGIVGPQGPPGPKGEPGLQGFPGKPGFLGEVGPPGMRGLPGPIGPKGEAGQKGVPGLPGVPGLLGPKGEPGIPGDQGLQGPPGIPGIGGPSGPIGPPGIPGPKGEPGLPGPPGFPGIGKPGVAGLHGPPGKPGALGPQGQPGLPGPPGPPGPPGPPAVMPPTPPPQGEYLPDMGLGIDGVKPPHAYGAKKGKNGGPAYEMPAFTAELTAPFPPVGAPVKFNKLLYNGRQNYNPQTGIFTCEVPGVYYFAYHVHCKGGNVWVALFKNNEPVMYTYDEYKKGFLDQASGSAVLLLRPGDRVFLQMPSEQAAGLYAGQYVHSSFSGYLLYPM

Target ORF information:

RefSeq Version	NM_020351.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VIII alpha 1 chain (COL8A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020351.4

ORF Insert Sequence:

ATGGCTGTGC TGCCTGGCCC TCTGCAGCTG CTGGGAGTGC TGCTTACCAT TTCCCTGAGT 
TCCATCAGGC TCATTCAGGC TGGTGCCTAC TATGGGATCA AGCCGCTGCC ACCTCAAATT 
CCTCCTCAGA TGCCACCACA AATTCCACAA TACCAGCCCC TGGGTCAGCA AGTACCTCAC 
ATGCCTTTGG CCAAAGATGG CCTTGCCATG GGCAAGGAGA TGCCCCACTT GCAGTATGGC 
AAAGAGTATC CACACCTACC CCAATATATG AAGGAAATTC AACCGGCGCC AAGAATGGGC 
AAGGAAGCCG TACCCAAGAA AGGCAAAGAA ATACCATTAG CCAGTTTACG AGGGGAACAA 
GGTCCCCGTG GAGAGCCTGG CCCAAGAGGA CCACCTGGGC CCCCTGGTTT GCCAGGTCAT 
GGGATACCTG GAATTAAAGG AAAACCAGGG CCACAGGGAT ATCCAGGAGT TGGAAAGCCA 
GGTATGCCTG GAATGCCAGG GAAGCCAGGA GCCATGGGCA TGCCTGGGGC AAAAGGAGAA 
ATTGGACAGA AAGGGGAAAT TGGGCCTATG GGGATCCCAG GACCACAAGG ACCTCCAGGG 
CCTCATGGAC TTCCTGGCAT TGGGAAGCCA GGTGGGCCAG GGTTACCAGG GCAACCAGGA 
CCAAAGGGTG ATCGAGGACC CAAAGGACTA CCAGGACCTC AAGGCCTTCG GGGTCCTAAA 
GGAGACAAGG GCTTCGGGAT GCCAGGTGCG CCAGGTGTAA AGGGGCCTCC AGGGATGCAC 
GGCCCTCCCG GCCCTGTTGG ACTGCCAGGA GTGGGCAAAC CAGGAGTGAC AGGCTTCCCT 
GGGCCCCAGG GCCCCCTGGG AAAGCCAGGG GCTCCAGGAG AACCTGGGCC ACAAGGCCCT 
ATTGGGGTAC CGGGGGTTCA AGGACCTCCT GGGATACCCG GAATTGGAAA GCCAGGCCAG 
GATGGGATCC CAGGCCAGCC AGGATTTCCA GGTGGCAAAG GGGAGCAAGG ACTGCCAGGG 
CTACCAGGAC CCCCAGGCCT TCCAGGGATT GGGAAACCAG GCTTCCCAGG ACCCAAAGGT 
GACCGGGGCA TGGGAGGTGT TCCTGGGGCT CTTGGACCAA GAGGGGAGAA AGGACCAATA 
GGTGCCCCAG GAATAGGGGG TCCTCCAGGA GAGCCAGGCC TGCCTGGAAT CCCAGGTCCT 
ATGGGCCCTC CAGGTGCTAT TGGTTTTCCT GGACCCAAAG GAGAAGGTGG GATTGTAGGG 
CCACAGGGGC CACCAGGTCC CAAGGGTGAG CCAGGGCTTC AAGGCTTCCC AGGAAAGCCA 
GGTTTCCTTG GTGAAGTAGG GCCTCCTGGC ATGAGGGGTT TGCCAGGTCC CATAGGGCCC 
AAGGGGGAAG CTGGGCAAAA AGGTGTACCA GGACTCCCTG GTGTTCCAGG GCTTCTCGGA 
CCTAAGGGAG AGCCAGGAAT CCCAGGGGAT CAGGGTTTAC AGGGCCCCCC AGGTATCCCA 
GGGATTGGGG GCCCTAGTGG CCCCATTGGA CCACCTGGGA TTCCAGGCCC CAAAGGGGAG 
CCGGGCCTCC CAGGGCCCCC TGGGTTCCCT GGTATAGGGA AACCCGGAGT GGCAGGACTT 
CATGGCCCCC CAGGGAAGCC TGGTGCCCTT GGTCCTCAAG GCCAGCCTGG CCTTCCAGGA 
CCCCCAGGCC CTCCAGGACC TCCAGGACCC CCAGCTGTGA TGCCCCCTAC ACCACCACCC 
CAGGGAGAGT ATCTGCCAGA TATGGGGCTG GGAATTGATG GCGTGAAACC CCCCCATGCC 
TACGGGGCTA AGAAAGGCAA GAATGGAGGG CCAGCCTATG AGATGCCTGC ATTTACCGCC 
GAGCTAACCG CACCTTTCCC ACCGGTGGGG GCCCCAGTGA AGTTTAACAA ACTGCTGTAT 
AACGGCAGAC AGAACTACAA CCCGCAGACA GGCATCTTCA CCTGTGAGGT CCCTGGTGTC 
TACTACTTTG CATACCACGT TCACTGCAAG GGGGGCAACG TGTGGGTTGC TCTATTCAAG 
AACAACGAGC CCGTGATGTA CACGTACGAC GAGTACAAAA AGGGCTTCCT GGACCAGGCA 
TCTGGGAGTG CAGTGCTGCT GCTCAGGCCC GGAGACCGGG TGTTCCTCCA GATGCCCTCA 
GAACAGGCTG CAGGACTGTA TGCCGGGCAG TATGTCCACT CCTCCTTTTC AGGATATTTA 
TTGTATCCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL8A1 ( NM_001850.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001841.2 Homo sapiens collagen type VIII alpha 1 chain (COL8A1), transcript variant 1, mRNA.

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CloneID	OHu28226
Clone ID Related Accession (Same CDS sequence)	NM_020351.3 , NM_001850.5 , NM_020351.4 , NM_001850.4
Accession Version	NM_001850.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	collagen alpha-1(VIII) chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK311449.1, BC013581.1, CB269463.1 and DA663372.1. On Dec 31, 2011 this sequence version replaced NM_001850.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013581.1, SRR1803613.147377.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGC TGCCTGGCCC TCTGCAGCTG CTGGGAGTGC TGCTTACCAT TTCCCTGAGT 
TCCATCAGGC TCATTCAGGC TGGTGCCTAC TATGGGATCA AGCCGCTGCC ACCTCAAATT 
CCTCCTCAGA TGCCACCACA AATTCCACAA TACCAGCCCC TGGGTCAGCA AGTACCTCAC 
ATGCCTTTGG CCAAAGATGG CCTTGCCATG GGCAAGGAGA TGCCCCACTT GCAGTATGGC 
AAAGAGTATC CACACCTACC CCAATATATG AAGGAAATTC AACCGGCGCC AAGAATGGGC 
AAGGAAGCCG TACCCAAGAA AGGCAAAGAA ATACCATTAG CCAGTTTACG AGGGGAACAA 
GGTCCCCGTG GAGAGCCTGG CCCAAGAGGA CCACCTGGGC CCCCTGGTTT GCCAGGTCAT 
GGGATACCTG GAATTAAAGG AAAACCAGGG CCACAGGGAT ATCCAGGAGT TGGAAAGCCA 
GGTATGCCTG GAATGCCAGG GAAGCCAGGA GCCATGGGCA TGCCTGGGGC AAAAGGAGAA 
ATTGGACAGA AAGGGGAAAT TGGGCCTATG GGGATCCCAG GACCACAAGG ACCTCCAGGG 
CCTCATGGAC TTCCTGGCAT TGGGAAGCCA GGTGGGCCAG GGTTACCAGG GCAACCAGGA 
CCAAAGGGTG ATCGAGGACC CAAAGGACTA CCAGGACCTC AAGGCCTTCG GGGTCCTAAA 
GGAGACAAGG GCTTCGGGAT GCCAGGTGCG CCAGGTGTAA AGGGGCCTCC AGGGATGCAC 
GGCCCTCCCG GCCCTGTTGG ACTGCCAGGA GTGGGCAAAC CAGGAGTGAC AGGCTTCCCT 
GGGCCCCAGG GCCCCCTGGG AAAGCCAGGG GCTCCAGGAG AACCTGGGCC ACAAGGCCCT 
ATTGGGGTAC CGGGGGTTCA AGGACCTCCT GGGATACCCG GAATTGGAAA GCCAGGCCAG 
GATGGGATCC CAGGCCAGCC AGGATTTCCA GGTGGCAAAG GGGAGCAAGG ACTGCCAGGG 
CTACCAGGAC CCCCAGGCCT TCCAGGGATT GGGAAACCAG GCTTCCCAGG ACCCAAAGGT 
GACCGGGGCA TGGGAGGTGT TCCTGGGGCT CTTGGACCAA GAGGGGAGAA AGGACCAATA 
GGTGCCCCAG GAATAGGGGG TCCTCCAGGA GAGCCAGGCC TGCCTGGAAT CCCAGGTCCT 
ATGGGCCCTC CAGGTGCTAT TGGTTTTCCT GGACCCAAAG GAGAAGGTGG GATTGTAGGG 
CCACAGGGGC CACCAGGTCC CAAGGGTGAG CCAGGGCTTC AAGGCTTCCC AGGAAAGCCA 
GGTTTCCTTG GTGAAGTAGG GCCTCCTGGC ATGAGGGGTT TGCCAGGTCC CATAGGGCCC 
AAGGGGGAAG CTGGGCAAAA AGGTGTACCA GGACTCCCTG GTGTTCCAGG GCTTCTCGGA 
CCTAAGGGAG AGCCAGGAAT CCCAGGGGAT CAGGGTTTAC AGGGCCCCCC AGGTATCCCA 
GGGATTGGGG GCCCTAGTGG CCCCATTGGA CCACCTGGGA TTCCAGGCCC CAAAGGGGAG 
CCGGGCCTCC CAGGGCCCCC TGGGTTCCCT GGTATAGGGA AACCCGGAGT GGCAGGACTT 
CATGGCCCCC CAGGGAAGCC TGGTGCCCTT GGTCCTCAAG GCCAGCCTGG CCTTCCAGGA 
CCCCCAGGCC CTCCAGGACC TCCAGGACCC CCAGCTGTGA TGCCCCCTAC ACCACCACCC 
CAGGGAGAGT ATCTGCCAGA TATGGGGCTG GGAATTGATG GCGTGAAACC CCCCCATGCC 
TACGGGGCTA AGAAAGGCAA GAATGGAGGG CCAGCCTATG AGATGCCTGC ATTTACCGCC 
GAGCTAACCG CACCTTTCCC ACCGGTGGGG GCCCCAGTGA AGTTTAACAA ACTGCTGTAT 
AACGGCAGAC AGAACTACAA CCCGCAGACA GGCATCTTCA CCTGTGAGGT CCCTGGTGTC 
TACTACTTTG CATACCACGT TCACTGCAAG GGGGGCAACG TGTGGGTTGC TCTATTCAAG 
AACAACGAGC CCGTGATGTA CACGTACGAC GAGTACAAAA AGGGCTTCCT GGACCAGGCA 
TCTGGGAGTG CAGTGCTGCT GCTCAGGCCC GGAGACCGGG TGTTCCTCCA GATGCCCTCA 
GAACAGGCTG CAGGACTGTA TGCCGGGCAG TATGTCCACT CCTCCTTTTC AGGATATTTA 
TTGTATCCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001841.2
CDS	260..2494
Translation	MAVLPGPLQLLGVLLTISLSSIRLIQAGAYYGIKPLPPQIPPQMPPQIPQYQPLGQQVPHMPLAKDGLAMGKEMPHLQYGKEYPHLPQYMKEIQPAPRMGKEAVPKKGKEIPLASLRGEQGPRGEPGPRGPPGPPGLPGHGIPGIKGKPGPQGYPGVGKPGMPGMPGKPGAMGMPGAKGEIGQKGEIGPMGIPGPQGPPGPHGLPGIGKPGGPGLPGQPGPKGDRGPKGLPGPQGLRGPKGDKGFGMPGAPGVKGPPGMHGPPGPVGLPGVGKPGVTGFPGPQGPLGKPGAPGEPGPQGPIGVPGVQGPPGIPGIGKPGQDGIPGQPGFPGGKGEQGLPGLPGPPGLPGIGKPGFPGPKGDRGMGGVPGALGPRGEKGPIGAPGIGGPPGEPGLPGIPGPMGPPGAIGFPGPKGEGGIVGPQGPPGPKGEPGLQGFPGKPGFLGEVGPPGMRGLPGPIGPKGEAGQKGVPGLPGVPGLLGPKGEPGIPGDQGLQGPPGIPGIGGPSGPIGPPGIPGPKGEPGLPGPPGFPGIGKPGVAGLHGPPGKPGALGPQGQPGLPGPPGPPGPPGPPAVMPPTPPPQGEYLPDMGLGIDGVKPPHAYGAKKGKNGGPAYEMPAFTAELTAPFPPVGAPVKFNKLLYNGRQNYNPQTGIFTCEVPGVYYFAYHVHCKGGNVWVALFKNNEPVMYTYDEYKKGFLDQASGSAVLLLRPGDRVFLQMPSEQAAGLYAGQYVHSSFSGYLLYPM

Target ORF information:

RefSeq Version	NM_001850.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VIII alpha 1 chain (COL8A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001850.4

ORF Insert Sequence:

ATGGCTGTGC TGCCTGGCCC TCTGCAGCTG CTGGGAGTGC TGCTTACCAT TTCCCTGAGT 
TCCATCAGGC TCATTCAGGC TGGTGCCTAC TATGGGATCA AGCCGCTGCC ACCTCAAATT 
CCTCCTCAGA TGCCACCACA AATTCCACAA TACCAGCCCC TGGGTCAGCA AGTACCTCAC 
ATGCCTTTGG CCAAAGATGG CCTTGCCATG GGCAAGGAGA TGCCCCACTT GCAGTATGGC 
AAAGAGTATC CACACCTACC CCAATATATG AAGGAAATTC AACCGGCGCC AAGAATGGGC 
AAGGAAGCCG TACCCAAGAA AGGCAAAGAA ATACCATTAG CCAGTTTACG AGGGGAACAA 
GGTCCCCGTG GAGAGCCTGG CCCAAGAGGA CCACCTGGGC CCCCTGGTTT GCCAGGTCAT 
GGGATACCTG GAATTAAAGG AAAACCAGGG CCACAGGGAT ATCCAGGAGT TGGAAAGCCA 
GGTATGCCTG GAATGCCAGG GAAGCCAGGA GCCATGGGCA TGCCTGGGGC AAAAGGAGAA 
ATTGGACAGA AAGGGGAAAT TGGGCCTATG GGGATCCCAG GACCACAAGG ACCTCCAGGG 
CCTCATGGAC TTCCTGGCAT TGGGAAGCCA GGTGGGCCAG GGTTACCAGG GCAACCAGGA 
CCAAAGGGTG ATCGAGGACC CAAAGGACTA CCAGGACCTC AAGGCCTTCG GGGTCCTAAA 
GGAGACAAGG GCTTCGGGAT GCCAGGTGCG CCAGGTGTAA AGGGGCCTCC AGGGATGCAC 
GGCCCTCCCG GCCCTGTTGG ACTGCCAGGA GTGGGCAAAC CAGGAGTGAC AGGCTTCCCT 
GGGCCCCAGG GCCCCCTGGG AAAGCCAGGG GCTCCAGGAG AACCTGGGCC ACAAGGCCCT 
ATTGGGGTAC CGGGGGTTCA AGGACCTCCT GGGATACCCG GAATTGGAAA GCCAGGCCAG 
GATGGGATCC CAGGCCAGCC AGGATTTCCA GGTGGCAAAG GGGAGCAAGG ACTGCCAGGG 
CTACCAGGAC CCCCAGGCCT TCCAGGGATT GGGAAACCAG GCTTCCCAGG ACCCAAAGGT 
GACCGGGGCA TGGGAGGTGT TCCTGGGGCT CTTGGACCAA GAGGGGAGAA AGGACCAATA 
GGTGCCCCAG GAATAGGGGG TCCTCCAGGA GAGCCAGGCC TGCCTGGAAT CCCAGGTCCT 
ATGGGCCCTC CAGGTGCTAT TGGTTTTCCT GGACCCAAAG GAGAAGGTGG GATTGTAGGG 
CCACAGGGGC CACCAGGTCC CAAGGGTGAG CCAGGGCTTC AAGGCTTCCC AGGAAAGCCA 
GGTTTCCTTG GTGAAGTAGG GCCTCCTGGC ATGAGGGGTT TGCCAGGTCC CATAGGGCCC 
AAGGGGGAAG CTGGGCAAAA AGGTGTACCA GGACTCCCTG GTGTTCCAGG GCTTCTCGGA 
CCTAAGGGAG AGCCAGGAAT CCCAGGGGAT CAGGGTTTAC AGGGCCCCCC AGGTATCCCA 
GGGATTGGGG GCCCTAGTGG CCCCATTGGA CCACCTGGGA TTCCAGGCCC CAAAGGGGAG 
CCGGGCCTCC CAGGGCCCCC TGGGTTCCCT GGTATAGGGA AACCCGGAGT GGCAGGACTT 
CATGGCCCCC CAGGGAAGCC TGGTGCCCTT GGTCCTCAAG GCCAGCCTGG CCTTCCAGGA 
CCCCCAGGCC CTCCAGGACC TCCAGGACCC CCAGCTGTGA TGCCCCCTAC ACCACCACCC 
CAGGGAGAGT ATCTGCCAGA TATGGGGCTG GGAATTGATG GCGTGAAACC CCCCCATGCC 
TACGGGGCTA AGAAAGGCAA GAATGGAGGG CCAGCCTATG AGATGCCTGC ATTTACCGCC 
GAGCTAACCG CACCTTTCCC ACCGGTGGGG GCCCCAGTGA AGTTTAACAA ACTGCTGTAT 
AACGGCAGAC AGAACTACAA CCCGCAGACA GGCATCTTCA CCTGTGAGGT CCCTGGTGTC 
TACTACTTTG CATACCACGT TCACTGCAAG GGGGGCAACG TGTGGGTTGC TCTATTCAAG 
AACAACGAGC CCGTGATGTA CACGTACGAC GAGTACAAAA AGGGCTTCCT GGACCAGGCA 
TCTGGGAGTG CAGTGCTGCT GCTCAGGCCC GGAGACCGGG TGTTCCTCCA GATGCCCTCA 
GAACAGGCTG CAGGACTGTA TGCCGGGCAG TATGTCCACT CCTCCTTTTC AGGATATTTA 
TTGTATCCCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Journal of proteome research16(8)3083-3091(2017 Aug)
Naba A,Pearce OMT,Del Rosario A,Ma D,Ding H,Rajeeve V,Cutillas PR,Balkwill FR,Hynes RO

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COL8A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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