COL7A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL7A1
Entrez Gene ID	1294
Full Name	collagen type VII alpha 1 chain
Synonyms	EBD1,EBDCT,EBR1,NDNC8
General protein information	Preferred Names collagen type VII alpha 1 chain Names collagen alpha-1(VII) chain LC collagen collagen VII, alpha-1 polypeptide collagen, type VII, alpha 1 long-chain collagen
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.31
Summary	This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq, Jul 2008].
Disorder MIM:	120120
Disorder Html:	Epidermolysis bullosa dystrophica, AD, 131750 (3); Epidermolysis bullosa dystrophica, AR, 226600 (3); Epidermolysis bullosa, pretibial, 131850 (3); EBD, Bart type, 132000 (3); EBD, localisata variant (3); Transient bullous of the newborn, 131705 (3); Epidermolysis bullosa pruriginosa, 604129 (3); Toenail dystrophy, isolated, 607523 (3); EBD inversa, 226600 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011533337.1	XP_011531639.1	collagen alpha-1(VII) chain isoform X1
XM_017005689.1	XP_016861178.1	collagen alpha-1(VII) chain isoform X3
XM_017005690.1	XP_016861179.1	collagen alpha-1(VII) chain isoform X4
NM_000094.3	NP_000085.1	collagen alpha-1(VII) chain precursor
XM_017005688.1	XP_016861177.1	collagen alpha-1(VII) chain isoform X2
XM_017005692.1	XP_016861181.1	collagen alpha-1(VII) chain isoform X6
XM_017005691.1	XP_016861180.1	collagen alpha-1(VII) chain isoform X5

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col7a1	NP_001100328.1
Bos taurus (cattle)	COL7A1	XP_002697097.2
Mus musculus (house mouse)	Col7a1	NP_031764.2
Gallus gallus (chicken)	COL7A1	XP_425157.4
Xenopus tropicalis (tropical clawed frog)	col7a1	XP_002933125.2
Canis lupus familiaris (dog)	COL7A1	NP_001002980.1
Danio rerio (zebrafish)	col7a1	XP_002662551.3
Homo sapiens (human)	COL7A1	NP_000085.1
Pan troglodytes (chimpanzee)	COL7A1	XP_003950149.1

Gene Ontology

The following COL7A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL7A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18956	NM_000094.3 Latest version!	Homo sapiens collagen type VII alpha 1 chain (COL7A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$1079.50-$1511.30 $2159.00
OHu18956	XM_011533337.1 Latest version!	Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$1079.50-$1511.30 $2159.00
OHu93793	XM_017005688.1 Latest version!	Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu93794	XM_017005689.1 Latest version!	Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu93795	XM_017005690.1 Latest version!	Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu93796	XM_017005691.1 Latest version!	Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu93797	XM_017005692.1 Latest version!	Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL7A1 ( NM_000094.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000085.1 Homo sapiens collagen type VII alpha 1 chain (COL7A1), mRNA.

Price & Availability↑

CloneID	OHu18956
Clone ID Related Accession (Same CDS sequence)	XM_011533337.1 , NM_000094.3
Accession Version	NM_000094.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	collagen alpha-1(VII) chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L02870.1, BQ300155.1, S51236.1, M65158.1 and AB209645.1. This sequence is a reference standard in the RefSeqGene project. On Sep 20, 2007 this sequence version replaced NM_000094.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L02870.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGTCA GCCAGGCCCT 
AGTGGAGAGC GGGGTCTGGC AGGCCCCCCA GGGAGAGAAG GAATCCCAGG ACCCCTGGGG 
CCACCTGGAC CACCGGGGTC AGTGGGACCA CCTGGGGCCT CTGGACTCAA AGGAGACAAG 
GGAGACCCTG GAGTAGGGCT GCCTGGGCCC CGAGGCGAGC GTGGGGAGCC AGGCATCCGG 
GGTGAAGATG GCCGCCCCGG CCAGGAGGGA CCCCGAGGAC TCACGGGGCC CCCTGGCAGC 
AGGGGAGAGC GTGGGGAGAA GGGTGATGTT GGGAGTGCAG GACTAAAGGG TGACAAGGGA 
GACTCAGCTG TGATCCTGGG GCCTCCAGGC CCACGGGGTG CCAAGGGGGA CATGGGTGAA 
CGAGGGCCTC GGGGCTTGGA TGGTGACAAA GGACCTCGGG GAGACAATGG GGACCCTGGT 
GACAAGGGCA GCAAGGGAGA GCCTGGTGAC AAGGGCTCAG CCGGGTTGCC AGGACTGCGT 
GGACTCCTGG GACCCCAGGG TCAACCTGGT GCAGCAGGGA TCCCTGGTGA CCCGGGATCC 
CCAGGAAAGG ATGGAGTGCC TGGTATCCGA GGAGAAAAAG GAGATGTTGG CTTCATGGGT 
CCCCGGGGCC TCAAGGGTGA ACGGGGAGTG AAGGGAGCCT GTGGCCTTGA TGGAGAGAAG 
GGAGACAAGG GAGAAGCTGG TCCCCCAGGC CGCCCCGGGC TGGCAGGACA CAAAGGAGAG 
ATGGGGGAGC CTGGTGTGCC GGGCCAGTCG GGGGCCCCTG GCAAGGAGGG CCTGATCGGT 
CCCAAGGGTG ACCGAGGCTT TGACGGGCAG CCAGGCCCCA AGGGTGACCA GGGCGAGAAA 
GGGGAGCGGG GAACCCCAGG AATTGGGGGC TTCCCAGGCC CCAGTGGAAA TGATGGCTCT 
GCTGGTCCCC CAGGGCCACC TGGCAGTGTT GGTCCCAGAG GCCCCGAAGG ACTTCAGGGC 
CAGAAGGGTG AGCGAGGTCC CCCCGGAGAG AGAGTGGTGG GGGCTCCTGG GGTCCCTGGA 
GCTCCTGGCG AGAGAGGGGA GCAGGGGCGG CCAGGGCCTG CCGGTCCTCG AGGCGAGAAG 
GGAGAAGCTG CACTGACGGA GGATGACATC CGGGGCTTTG TGCGCCAAGA GATGAGTCAG 
CACTGTGCCT GCCAGGGCCA GTTCATCGCA TCTGGATCAC GACCCCTCCC TAGTTATGCT 
GCAGACACTG CCGGCTCCCA GCTCCATGCT GTGCCTGTGC TCCGCGTCTC TCATGCAGAG 
GAGGAAGAGC GGGTACCCCC TGAGGATGAT GAGTACTCTG AATACTCCGA GTATTCTGTG 
GAGGAGTACC AGGACCCTGA AGCTCCTTGG GATAGTGATG ACCCCTGTTC CCTGCCACTG 
GATGAGGGCT CCTGCACTGC CTACACCCTG CGCTGGTACC ATCGGGCTGT GACAGGCAGC 
ACAGAGGCCT GTCACCCTTT TGTCTATGGT GGCTGTGGAG GGAATGCCAA CCGTTTTGGG 
ACCCGTGAGG CCTGCGAGCG CCGCTGCCCA CCCCGGGTGG TCCAGAGCCA GGGGACAGGT 
ACTGCCCAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000085.1
CDS	2..8836
Translation	MTLRLLVAALCAGILAEAPRVRAQHRERVTCTRLYAADIVFLLDGSSSIGRSNFREVRSFLEGLVLPFSGAASAQGVRFATVQYSDDPRTEFGLDALGSGGDVIRAIRELSYKGGNTRTGAAILHVADHVFLPQLARPGVPKVCILITDGKSQDLVDTAAQRLKGQGVKLFAVGIKNADPEELKRVASQPTSDFFFFVNDFSILRTLLPLVSRRVCTTAGGVPVTRPPDDSTSAPRDLVLSEPSSQSLRVQWTAASGPVTGYKVQYTPLTGLGQPLPSERQEVNVPAGETSVRLRGLRPLTEYQVTVIALYANSIGEAVSGTARTTALEGPELTIQNTTAHSLLVAWRSVPGATGYRVTWRVLSGGPTQQQELGPGQGSVLLRDLEPGTDYEVTVSTLFGRSVGPATSLMARTDASVEQTLRPVILGPTSILLSWNLVPEARGYRLEWRRETGLEPPQKVVLPSDVTRYQLDGLQPGTEYRLTLYTLLEGHEVATPATVVPTGPELPVSPVTDLQATELPGQRVRVSWSPVPGATQYRIIVRSTQGVERTLVLPGSQTAFDLDDVQAGLSYTVRVSARVGPREGSASVLTVRREPETPLAVPGLRVVVSDATRVRVAWGPVPGASGFRISWSTGSGPESSQTLPPDSTATDITGLQPGTTYQVAVSVLRGREEGPAAVIVARTDPLGPVRTVHVTQASSSSVTITWTRVPGATGYRVSWHSAHGPEKSQLVSGEATVAELDGLEPDTEYTVHVRAHVAGVDGPPASVVVRTAPEPVGRVSRLQILNASSDVLRITWVGVTGATAYRLAWGRSEGGPMRHQILPGNTDSAEIRGLEGGVSYSVRVTALVGDREGTPVSIVVTTPPEAPPALGTLHVVQRGEHSLRLRWEPVPRAQGFLLHWQPEGGQEQSRVLGPELSSYHLDGLEPATQYRVRLSVLGPAGEGPSAEVTARTESPRVPSIELRVVDTSIDSVTLAWTPVSRASSYILSWRPLRGPGQEVPGSPQTLPGISSSQRVTGLEPGVSYIFSLTPVLDGVRGPEASVTQTPVCPRGLADVVFLPHATQDNAHRAEATRRVLERLVLALGPLGPQAVQVGLLSYSHRPSPLFPLNGSHDLGIILQRIRDMPYMDPSGNNLGTAVVTAHRYMLAPDAPGRRQHVPGVMVLLVDEPLRGDIFSPIREAQASGLNVVMLGMAGADPEQLRRLAPGMDSVQTFFAVDDGPSLDQAVSGLATALCQASFTTQPRPEPCPVYCPKGQKGEPGEMGLRGQVGPPGDPGLPGRTGAPGPQGPPGSATAKGERGFPGADGRPGSPGRAGNPGTPGAPGLKGSPGLPGPRGDPGERGPRGPKGEPGAPGQVIGGEGPGLPGRKGDPGPSGPPGPRGPLGDPGPRGPPGLPGTAMKGDKGDRGERGPPGPGEGGIAPGEPGLPGLPGSPGPQGPVGPPGKKGEKGDSEDGAPGLPGQPGSPGEQGPRGPPGAIGPKGDRGFPGPLGEAGEKGERGPPGPAGSRGLPGVAGRPGAKGPEGPPGPTGRQGEKGEPGRPGDPAVVGPAVAGPKGEKGDVGPAGPRGATGVQGERGPPGLVLPGDPGPKGDPGDRGPIGLTGRAGPPGDSGPPGEKGDPGRPGPPGPVGPRGRDGEVGEKGDEGPPGDPGLPGKAGERGLRGAPGVRGPVGEKGDQGDPGEDGRNGSPGSSGPKGDRGEPGPPGPPGRLVDTGPGAREKGEPGDRGQEGPRGPKGDPGLPGAPGERGIEGFRGPPGPQGDPGVRGPAGEKGDRGPPGLDGRSGLDGKPGAAGPSGPNGAAGKAGDPGRDGLPGLRGEQGLPGPSGPPGLPGKPGEDGKPGLNGKNGEPGDPGEDGRKGEKGDSGASGREGRDGPKGERGAPGILGPQGPPGLPGPVGPPGQGFPGVPGGTGPKGDRGETGSKGEQGLPGERGLRGEPGSVPNVDRLLETAGIKASALREIVETWDESSGSFLPVPERRRGPKGDSGEQGPPGKEGPIGFPGERGLKGDRGDPGPQGPPGLALGERGPPGPSGLAGEPGKPGIPGLPGRAGGVGEAGRPGERGERGEKGERGEQGRDGPPGLPGTPGPPGPPGPKVSVDEPGPGLSGEQGPPGLKGAKGEPGSNGDQGPKGDRGVPGIKGDRGEPGPRGQDGNPGLPGERGMAGPEGKPGLQGPRGPPGPVGGHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLTGPTGAVGLPGPPGPSGLVGPQGSPGLPGQVGETGKPGAPGRDGASGKDGDRGSPGVPGSPGLPGPVGPKGEPGPTGAPGQAVVGLPGAKGEKGAPGGLAGDLVGEPGAKGDRGLPGPRGEKGEAGRAGEPGDPGEDGQKGAPGPKGFKGDPGVGVPGSPGPPGPPGVKGDLGLPGLPGAPGVVGFPGQTGPRGEMGQPGPSGERGLAGPPGREGIPGPLGPPGPPGSVGPPGASGLKGDKGDPGVGLPGPRGERGEPGIRGEDGRPGQEGPRGLTGPPGSRGERGEKGDVGSAGLKGDKGDSAVILGPPGPRGAKGDMGERGPRGLDGDKGPRGDNGDPGDKGSKGEPGDKGSAGLPGLRGLLGPQGQPGAAGIPGDPGSPGKDGVPGIRGEKGDVGFMGPRGLKGERGVKGACGLDGEKGDKGEAGPPGRPGLAGHKGEMGEPGVPGQSGAPGKEGLIGPKGDRGFDGQPGPKGDQGEKGERGTPGIGGFPGPSGNDGSAGPPGPPGSVGPRGPEGLQGQKGERGPPGERVVGAPGVPGAPGERGEQGRPGPAGPRGEKGEAALTEDDIRGFVRQEMSQHCACQGQFIASGSRPLPSYAADTAGSQLHAVPVLRVSHAEEEERVPPEDDEYSEYSEYSVEEYQDPEAPWDSDDPCSLPLDEGSCTAYTLRWYHRAVTGSTEACHPFVYGGCGGNANRFGTREACERRCPPRVVQSQGTGTAQD

Target ORF information:

RefSeq Version	NM_000094.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VII alpha 1 chain (COL7A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000094.3

ORF Insert Sequence:

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGTCA GCCAGGCCCT 
AGTGGAGAGC GGGGTCTGGC AGGCCCCCCA GGGAGAGAAG GAATCCCAGG ACCCCTGGGG 
CCACCTGGAC CACCGGGGTC AGTGGGACCA CCTGGGGCCT CTGGACTCAA AGGAGACAAG 
GGAGACCCTG GAGTAGGGCT GCCTGGGCCC CGAGGCGAGC GTGGGGAGCC AGGCATCCGG 
GGTGAAGATG GCCGCCCCGG CCAGGAGGGA CCCCGAGGAC TCACGGGGCC CCCTGGCAGC 
AGGGGAGAGC GTGGGGAGAA GGGTGATGTT GGGAGTGCAG GACTAAAGGG TGACAAGGGA 
GACTCAGCTG TGATCCTGGG GCCTCCAGGC CCACGGGGTG CCAAGGGGGA CATGGGTGAA 
CGAGGGCCTC GGGGCTTGGA TGGTGACAAA GGACCTCGGG GAGACAATGG GGACCCTGGT 
GACAAGGGCA GCAAGGGAGA GCCTGGTGAC AAGGGCTCAG CCGGGTTGCC AGGACTGCGT 
GGACTCCTGG GACCCCAGGG TCAACCTGGT GCAGCAGGGA TCCCTGGTGA CCCGGGATCC 
CCAGGAAAGG ATGGAGTGCC TGGTATCCGA GGAGAAAAAG GAGATGTTGG CTTCATGGGT 
CCCCGGGGCC TCAAGGGTGA ACGGGGAGTG AAGGGAGCCT GTGGCCTTGA TGGAGAGAAG 
GGAGACAAGG GAGAAGCTGG TCCCCCAGGC CGCCCCGGGC TGGCAGGACA CAAAGGAGAG 
ATGGGGGAGC CTGGTGTGCC GGGCCAGTCG GGGGCCCCTG GCAAGGAGGG CCTGATCGGT 
CCCAAGGGTG ACCGAGGCTT TGACGGGCAG CCAGGCCCCA AGGGTGACCA GGGCGAGAAA 
GGGGAGCGGG GAACCCCAGG AATTGGGGGC TTCCCAGGCC CCAGTGGAAA TGATGGCTCT 
GCTGGTCCCC CAGGGCCACC TGGCAGTGTT GGTCCCAGAG GCCCCGAAGG ACTTCAGGGC 
CAGAAGGGTG AGCGAGGTCC CCCCGGAGAG AGAGTGGTGG GGGCTCCTGG GGTCCCTGGA 
GCTCCTGGCG AGAGAGGGGA GCAGGGGCGG CCAGGGCCTG CCGGTCCTCG AGGCGAGAAG 
GGAGAAGCTG CACTGACGGA GGATGACATC CGGGGCTTTG TGCGCCAAGA GATGAGTCAG 
CACTGTGCCT GCCAGGGCCA GTTCATCGCA TCTGGATCAC GACCCCTCCC TAGTTATGCT 
GCAGACACTG CCGGCTCCCA GCTCCATGCT GTGCCTGTGC TCCGCGTCTC TCATGCAGAG 
GAGGAAGAGC GGGTACCCCC TGAGGATGAT GAGTACTCTG AATACTCCGA GTATTCTGTG 
GAGGAGTACC AGGACCCTGA AGCTCCTTGG GATAGTGATG ACCCCTGTTC CCTGCCACTG 
GATGAGGGCT CCTGCACTGC CTACACCCTG CGCTGGTACC ATCGGGCTGT GACAGGCAGC 
ACAGAGGCCT GTCACCCTTT TGTCTATGGT GGCTGTGGAG GGAATGCCAA CCGTTTTGGG 
ACCCGTGAGG CCTGCGAGCG CCGCTGCCCA CCCCGGGTGG TCCAGAGCCA GGGGACAGGT 
ACTGCCCAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL7A1 ( XM_011533337.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531639.1 Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X1, mRNA.

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CloneID	OHu18956
Clone ID Related Accession (Same CDS sequence)	XM_011533337.1 , NM_000094.3
Accession Version	XM_011533337.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(VII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGTCA GCCAGGCCCT 
AGTGGAGAGC GGGGTCTGGC AGGCCCCCCA GGGAGAGAAG GAATCCCAGG ACCCCTGGGG 
CCACCTGGAC CACCGGGGTC AGTGGGACCA CCTGGGGCCT CTGGACTCAA AGGAGACAAG 
GGAGACCCTG GAGTAGGGCT GCCTGGGCCC CGAGGCGAGC GTGGGGAGCC AGGCATCCGG 
GGTGAAGATG GCCGCCCCGG CCAGGAGGGA CCCCGAGGAC TCACGGGGCC CCCTGGCAGC 
AGGGGAGAGC GTGGGGAGAA GGGTGATGTT GGGAGTGCAG GACTAAAGGG TGACAAGGGA 
GACTCAGCTG TGATCCTGGG GCCTCCAGGC CCACGGGGTG CCAAGGGGGA CATGGGTGAA 
CGAGGGCCTC GGGGCTTGGA TGGTGACAAA GGACCTCGGG GAGACAATGG GGACCCTGGT 
GACAAGGGCA GCAAGGGAGA GCCTGGTGAC AAGGGCTCAG CCGGGTTGCC AGGACTGCGT 
GGACTCCTGG GACCCCAGGG TCAACCTGGT GCAGCAGGGA TCCCTGGTGA CCCGGGATCC 
CCAGGAAAGG ATGGAGTGCC TGGTATCCGA GGAGAAAAAG GAGATGTTGG CTTCATGGGT 
CCCCGGGGCC TCAAGGGTGA ACGGGGAGTG AAGGGAGCCT GTGGCCTTGA TGGAGAGAAG 
GGAGACAAGG GAGAAGCTGG TCCCCCAGGC CGCCCCGGGC TGGCAGGACA CAAAGGAGAG 
ATGGGGGAGC CTGGTGTGCC GGGCCAGTCG GGGGCCCCTG GCAAGGAGGG CCTGATCGGT 
CCCAAGGGTG ACCGAGGCTT TGACGGGCAG CCAGGCCCCA AGGGTGACCA GGGCGAGAAA 
GGGGAGCGGG GAACCCCAGG AATTGGGGGC TTCCCAGGCC CCAGTGGAAA TGATGGCTCT 
GCTGGTCCCC CAGGGCCACC TGGCAGTGTT GGTCCCAGAG GCCCCGAAGG ACTTCAGGGC 
CAGAAGGGTG AGCGAGGTCC CCCCGGAGAG AGAGTGGTGG GGGCTCCTGG GGTCCCTGGA 
GCTCCTGGCG AGAGAGGGGA GCAGGGGCGG CCAGGGCCTG CCGGTCCTCG AGGCGAGAAG 
GGAGAAGCTG CACTGACGGA GGATGACATC CGGGGCTTTG TGCGCCAAGA GATGAGTCAG 
CACTGTGCCT GCCAGGGCCA GTTCATCGCA TCTGGATCAC GACCCCTCCC TAGTTATGCT 
GCAGACACTG CCGGCTCCCA GCTCCATGCT GTGCCTGTGC TCCGCGTCTC TCATGCAGAG 
GAGGAAGAGC GGGTACCCCC TGAGGATGAT GAGTACTCTG AATACTCCGA GTATTCTGTG 
GAGGAGTACC AGGACCCTGA AGCTCCTTGG GATAGTGATG ACCCCTGTTC CCTGCCACTG 
GATGAGGGCT CCTGCACTGC CTACACCCTG CGCTGGTACC ATCGGGCTGT GACAGGCAGC 
ACAGAGGCCT GTCACCCTTT TGTCTATGGT GGCTGTGGAG GGAATGCCAA CCGTTTTGGG 
ACCCGTGAGG CCTGCGAGCG CCGCTGCCCA CCCCGGGTGG TCCAGAGCCA GGGGACAGGT 
ACTGCCCAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531639.1
CDS	37..8871
Translation	MTLRLLVAALCAGILAEAPRVRAQHRERVTCTRLYAADIVFLLDGSSSIGRSNFREVRSFLEGLVLPFSGAASAQGVRFATVQYSDDPRTEFGLDALGSGGDVIRAIRELSYKGGNTRTGAAILHVADHVFLPQLARPGVPKVCILITDGKSQDLVDTAAQRLKGQGVKLFAVGIKNADPEELKRVASQPTSDFFFFVNDFSILRTLLPLVSRRVCTTAGGVPVTRPPDDSTSAPRDLVLSEPSSQSLRVQWTAASGPVTGYKVQYTPLTGLGQPLPSERQEVNVPAGETSVRLRGLRPLTEYQVTVIALYANSIGEAVSGTARTTALEGPELTIQNTTAHSLLVAWRSVPGATGYRVTWRVLSGGPTQQQELGPGQGSVLLRDLEPGTDYEVTVSTLFGRSVGPATSLMARTDASVEQTLRPVILGPTSILLSWNLVPEARGYRLEWRRETGLEPPQKVVLPSDVTRYQLDGLQPGTEYRLTLYTLLEGHEVATPATVVPTGPELPVSPVTDLQATELPGQRVRVSWSPVPGATQYRIIVRSTQGVERTLVLPGSQTAFDLDDVQAGLSYTVRVSARVGPREGSASVLTVRREPETPLAVPGLRVVVSDATRVRVAWGPVPGASGFRISWSTGSGPESSQTLPPDSTATDITGLQPGTTYQVAVSVLRGREEGPAAVIVARTDPLGPVRTVHVTQASSSSVTITWTRVPGATGYRVSWHSAHGPEKSQLVSGEATVAELDGLEPDTEYTVHVRAHVAGVDGPPASVVVRTAPEPVGRVSRLQILNASSDVLRITWVGVTGATAYRLAWGRSEGGPMRHQILPGNTDSAEIRGLEGGVSYSVRVTALVGDREGTPVSIVVTTPPEAPPALGTLHVVQRGEHSLRLRWEPVPRAQGFLLHWQPEGGQEQSRVLGPELSSYHLDGLEPATQYRVRLSVLGPAGEGPSAEVTARTESPRVPSIELRVVDTSIDSVTLAWTPVSRASSYILSWRPLRGPGQEVPGSPQTLPGISSSQRVTGLEPGVSYIFSLTPVLDGVRGPEASVTQTPVCPRGLADVVFLPHATQDNAHRAEATRRVLERLVLALGPLGPQAVQVGLLSYSHRPSPLFPLNGSHDLGIILQRIRDMPYMDPSGNNLGTAVVTAHRYMLAPDAPGRRQHVPGVMVLLVDEPLRGDIFSPIREAQASGLNVVMLGMAGADPEQLRRLAPGMDSVQTFFAVDDGPSLDQAVSGLATALCQASFTTQPRPEPCPVYCPKGQKGEPGEMGLRGQVGPPGDPGLPGRTGAPGPQGPPGSATAKGERGFPGADGRPGSPGRAGNPGTPGAPGLKGSPGLPGPRGDPGERGPRGPKGEPGAPGQVIGGEGPGLPGRKGDPGPSGPPGPRGPLGDPGPRGPPGLPGTAMKGDKGDRGERGPPGPGEGGIAPGEPGLPGLPGSPGPQGPVGPPGKKGEKGDSEDGAPGLPGQPGSPGEQGPRGPPGAIGPKGDRGFPGPLGEAGEKGERGPPGPAGSRGLPGVAGRPGAKGPEGPPGPTGRQGEKGEPGRPGDPAVVGPAVAGPKGEKGDVGPAGPRGATGVQGERGPPGLVLPGDPGPKGDPGDRGPIGLTGRAGPPGDSGPPGEKGDPGRPGPPGPVGPRGRDGEVGEKGDEGPPGDPGLPGKAGERGLRGAPGVRGPVGEKGDQGDPGEDGRNGSPGSSGPKGDRGEPGPPGPPGRLVDTGPGAREKGEPGDRGQEGPRGPKGDPGLPGAPGERGIEGFRGPPGPQGDPGVRGPAGEKGDRGPPGLDGRSGLDGKPGAAGPSGPNGAAGKAGDPGRDGLPGLRGEQGLPGPSGPPGLPGKPGEDGKPGLNGKNGEPGDPGEDGRKGEKGDSGASGREGRDGPKGERGAPGILGPQGPPGLPGPVGPPGQGFPGVPGGTGPKGDRGETGSKGEQGLPGERGLRGEPGSVPNVDRLLETAGIKASALREIVETWDESSGSFLPVPERRRGPKGDSGEQGPPGKEGPIGFPGERGLKGDRGDPGPQGPPGLALGERGPPGPSGLAGEPGKPGIPGLPGRAGGVGEAGRPGERGERGEKGERGEQGRDGPPGLPGTPGPPGPPGPKVSVDEPGPGLSGEQGPPGLKGAKGEPGSNGDQGPKGDRGVPGIKGDRGEPGPRGQDGNPGLPGERGMAGPEGKPGLQGPRGPPGPVGGHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLTGPTGAVGLPGPPGPSGLVGPQGSPGLPGQVGETGKPGAPGRDGASGKDGDRGSPGVPGSPGLPGPVGPKGEPGPTGAPGQAVVGLPGAKGEKGAPGGLAGDLVGEPGAKGDRGLPGPRGEKGEAGRAGEPGDPGEDGQKGAPGPKGFKGDPGVGVPGSPGPPGPPGVKGDLGLPGLPGAPGVVGFPGQTGPRGEMGQPGPSGERGLAGPPGREGIPGPLGPPGPPGSVGPPGASGLKGDKGDPGVGLPGPRGERGEPGIRGEDGRPGQEGPRGLTGPPGSRGERGEKGDVGSAGLKGDKGDSAVILGPPGPRGAKGDMGERGPRGLDGDKGPRGDNGDPGDKGSKGEPGDKGSAGLPGLRGLLGPQGQPGAAGIPGDPGSPGKDGVPGIRGEKGDVGFMGPRGLKGERGVKGACGLDGEKGDKGEAGPPGRPGLAGHKGEMGEPGVPGQSGAPGKEGLIGPKGDRGFDGQPGPKGDQGEKGERGTPGIGGFPGPSGNDGSAGPPGPPGSVGPRGPEGLQGQKGERGPPGERVVGAPGVPGAPGERGEQGRPGPAGPRGEKGEAALTEDDIRGFVRQEMSQHCACQGQFIASGSRPLPSYAADTAGSQLHAVPVLRVSHAEEEERVPPEDDEYSEYSEYSVEEYQDPEAPWDSDDPCSLPLDEGSCTAYTLRWYHRAVTGSTEACHPFVYGGCGGNANRFGTREACERRCPPRVVQSQGTGTAQD

Target ORF information:

RefSeq Version	XM_011533337.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533337.1

ORF Insert Sequence:

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGTCA GCCAGGCCCT 
AGTGGAGAGC GGGGTCTGGC AGGCCCCCCA GGGAGAGAAG GAATCCCAGG ACCCCTGGGG 
CCACCTGGAC CACCGGGGTC AGTGGGACCA CCTGGGGCCT CTGGACTCAA AGGAGACAAG 
GGAGACCCTG GAGTAGGGCT GCCTGGGCCC CGAGGCGAGC GTGGGGAGCC AGGCATCCGG 
GGTGAAGATG GCCGCCCCGG CCAGGAGGGA CCCCGAGGAC TCACGGGGCC CCCTGGCAGC 
AGGGGAGAGC GTGGGGAGAA GGGTGATGTT GGGAGTGCAG GACTAAAGGG TGACAAGGGA 
GACTCAGCTG TGATCCTGGG GCCTCCAGGC CCACGGGGTG CCAAGGGGGA CATGGGTGAA 
CGAGGGCCTC GGGGCTTGGA TGGTGACAAA GGACCTCGGG GAGACAATGG GGACCCTGGT 
GACAAGGGCA GCAAGGGAGA GCCTGGTGAC AAGGGCTCAG CCGGGTTGCC AGGACTGCGT 
GGACTCCTGG GACCCCAGGG TCAACCTGGT GCAGCAGGGA TCCCTGGTGA CCCGGGATCC 
CCAGGAAAGG ATGGAGTGCC TGGTATCCGA GGAGAAAAAG GAGATGTTGG CTTCATGGGT 
CCCCGGGGCC TCAAGGGTGA ACGGGGAGTG AAGGGAGCCT GTGGCCTTGA TGGAGAGAAG 
GGAGACAAGG GAGAAGCTGG TCCCCCAGGC CGCCCCGGGC TGGCAGGACA CAAAGGAGAG 
ATGGGGGAGC CTGGTGTGCC GGGCCAGTCG GGGGCCCCTG GCAAGGAGGG CCTGATCGGT 
CCCAAGGGTG ACCGAGGCTT TGACGGGCAG CCAGGCCCCA AGGGTGACCA GGGCGAGAAA 
GGGGAGCGGG GAACCCCAGG AATTGGGGGC TTCCCAGGCC CCAGTGGAAA TGATGGCTCT 
GCTGGTCCCC CAGGGCCACC TGGCAGTGTT GGTCCCAGAG GCCCCGAAGG ACTTCAGGGC 
CAGAAGGGTG AGCGAGGTCC CCCCGGAGAG AGAGTGGTGG GGGCTCCTGG GGTCCCTGGA 
GCTCCTGGCG AGAGAGGGGA GCAGGGGCGG CCAGGGCCTG CCGGTCCTCG AGGCGAGAAG 
GGAGAAGCTG CACTGACGGA GGATGACATC CGGGGCTTTG TGCGCCAAGA GATGAGTCAG 
CACTGTGCCT GCCAGGGCCA GTTCATCGCA TCTGGATCAC GACCCCTCCC TAGTTATGCT 
GCAGACACTG CCGGCTCCCA GCTCCATGCT GTGCCTGTGC TCCGCGTCTC TCATGCAGAG 
GAGGAAGAGC GGGTACCCCC TGAGGATGAT GAGTACTCTG AATACTCCGA GTATTCTGTG 
GAGGAGTACC AGGACCCTGA AGCTCCTTGG GATAGTGATG ACCCCTGTTC CCTGCCACTG 
GATGAGGGCT CCTGCACTGC CTACACCCTG CGCTGGTACC ATCGGGCTGT GACAGGCAGC 
ACAGAGGCCT GTCACCCTTT TGTCTATGGT GGCTGTGGAG GGAATGCCAA CCGTTTTGGG 
ACCCGTGAGG CCTGCGAGCG CCGCTGCCCA CCCCGGGTGG TCCAGAGCCA GGGGACAGGT 
ACTGCCCAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL7A1 ( XM_017005688.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861177.1 Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X2, mRNA.

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CloneID	OHu93793
Clone ID Related Accession (Same CDS sequence)	XM_017005688.1
Accession Version	XM_017005688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(VII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGTCA GCCAGGCCCT 
AGTGGAGAGC GGGGTCTGGC AGGCCCCCCA GGGAGAGAAG GAATCCCAGG ACCCCTGGGG 
CCACCTGGAC CACCGGGGTC AGTGGGACCA CCTGGGGCCT CTGGACTCAA AGGAGACAAG 
GGTGAAGATG GCCGCCCCGG CCAGGAGGGA CCCCGAGGAC TCACGGGGCC CCCTGGCAGC 
AGGGGAGAGC GTGGGGAGAA GGGTGATGTT GGGAGTGCAG GACTAAAGGG TGACAAGGGA 
GACTCAGCTG TGATCCTGGG GCCTCCAGGC CCACGGGGTG CCAAGGGGGA CATGGGTGAA 
CGAGGGCCTC GGGGCTTGGA TGGTGACAAA GGACCTCGGG GAGACAATGG GGACCCTGGT 
GACAAGGGCA GCAAGGGAGA GCCTGGTGAC AAGGGCTCAG CCGGGTTGCC AGGACTGCGT 
GGACTCCTGG GACCCCAGGG TCAACCTGGT GCAGCAGGGA TCCCTGGTGA CCCGGGATCC 
CCAGGAAAGG ATGGAGTGCC TGGTATCCGA GGAGAAAAAG GAGATGTTGG CTTCATGGGT 
CCCCGGGGCC TCAAGGGTGA ACGGGGAGTG AAGGGAGCCT GTGGCCTTGA TGGAGAGAAG 
GGAGACAAGG GAGAAGCTGG TCCCCCAGGC CGCCCCGGGC TGGCAGGACA CAAAGGAGAG 
ATGGGGGAGC CTGGTGTGCC GGGCCAGTCG GGGGCCCCTG GCAAGGAGGG CCTGATCGGT 
CCCAAGGGTG ACCGAGGCTT TGACGGGCAG CCAGGCCCCA AGGGTGACCA GGGCGAGAAA 
GGGGAGCGGG GAACCCCAGG AATTGGGGGC TTCCCAGGCC CCAGTGGAAA TGATGGCTCT 
GCTGGTCCCC CAGGGCCACC TGGCAGTGTT GGTCCCAGAG GCCCCGAAGG ACTTCAGGGC 
CAGAAGGGTG AGCGAGGTCC CCCCGGAGAG AGAGTGGTGG GGGCTCCTGG GGTCCCTGGA 
GCTCCTGGCG AGAGAGGGGA GCAGGGGCGG CCAGGGCCTG CCGGTCCTCG AGGCGAGAAG 
GGAGAAGCTG CACTGACGGA GGATGACATC CGGGGCTTTG TGCGCCAAGA GATGAGTCAG 
CACTGTGCCT GCCAGGGCCA GTTCATCGCA TCTGGATCAC GACCCCTCCC TAGTTATGCT 
GCAGACACTG CCGGCTCCCA GCTCCATGCT GTGCCTGTGC TCCGCGTCTC TCATGCAGAG 
GAGGAAGAGC GGGTACCCCC TGAGGATGAT GAGTACTCTG AATACTCCGA GTATTCTGTG 
GAGGAGTACC AGGACCCTGA AGCTCCTTGG GATAGTGATG ACCCCTGTTC CCTGCCACTG 
GATGAGGGCT CCTGCACTGC CTACACCCTG CGCTGGTACC ATCGGGCTGT GACAGGCAGC 
ACAGAGGCCT GTCACCCTTT TGTCTATGGT GGCTGTGGAG GGAATGCCAA CCGTTTTGGG 
ACCCGTGAGG CCTGCGAGCG CCGCTGCCCA CCCCGGGTGG TCCAGAGCCA GGGGACAGGT 
ACTGCCCAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861177.1
CDS	37..8811
Translation	MTLRLLVAALCAGILAEAPRVRAQHRERVTCTRLYAADIVFLLDGSSSIGRSNFREVRSFLEGLVLPFSGAASAQGVRFATVQYSDDPRTEFGLDALGSGGDVIRAIRELSYKGGNTRTGAAILHVADHVFLPQLARPGVPKVCILITDGKSQDLVDTAAQRLKGQGVKLFAVGIKNADPEELKRVASQPTSDFFFFVNDFSILRTLLPLVSRRVCTTAGGVPVTRPPDDSTSAPRDLVLSEPSSQSLRVQWTAASGPVTGYKVQYTPLTGLGQPLPSERQEVNVPAGETSVRLRGLRPLTEYQVTVIALYANSIGEAVSGTARTTALEGPELTIQNTTAHSLLVAWRSVPGATGYRVTWRVLSGGPTQQQELGPGQGSVLLRDLEPGTDYEVTVSTLFGRSVGPATSLMARTDASVEQTLRPVILGPTSILLSWNLVPEARGYRLEWRRETGLEPPQKVVLPSDVTRYQLDGLQPGTEYRLTLYTLLEGHEVATPATVVPTGPELPVSPVTDLQATELPGQRVRVSWSPVPGATQYRIIVRSTQGVERTLVLPGSQTAFDLDDVQAGLSYTVRVSARVGPREGSASVLTVRREPETPLAVPGLRVVVSDATRVRVAWGPVPGASGFRISWSTGSGPESSQTLPPDSTATDITGLQPGTTYQVAVSVLRGREEGPAAVIVARTDPLGPVRTVHVTQASSSSVTITWTRVPGATGYRVSWHSAHGPEKSQLVSGEATVAELDGLEPDTEYTVHVRAHVAGVDGPPASVVVRTAPEPVGRVSRLQILNASSDVLRITWVGVTGATAYRLAWGRSEGGPMRHQILPGNTDSAEIRGLEGGVSYSVRVTALVGDREGTPVSIVVTTPPEAPPALGTLHVVQRGEHSLRLRWEPVPRAQGFLLHWQPEGGQEQSRVLGPELSSYHLDGLEPATQYRVRLSVLGPAGEGPSAEVTARTESPRVPSIELRVVDTSIDSVTLAWTPVSRASSYILSWRPLRGPGQEVPGSPQTLPGISSSQRVTGLEPGVSYIFSLTPVLDGVRGPEASVTQTPVCPRGLADVVFLPHATQDNAHRAEATRRVLERLVLALGPLGPQAVQVGLLSYSHRPSPLFPLNGSHDLGIILQRIRDMPYMDPSGNNLGTAVVTAHRYMLAPDAPGRRQHVPGVMVLLVDEPLRGDIFSPIREAQASGLNVVMLGMAGADPEQLRRLAPGMDSVQTFFAVDDGPSLDQAVSGLATALCQASFTTQPRPEPCPVYCPKGQKGEPGEMGLRGQVGPPGDPGLPGRTGAPGPQGPPGSATAKGERGFPGADGRPGSPGRAGNPGTPGAPGLKGSPGLPGPRGDPGERGPRGPKGEPGAPGQVIGGEGPGLPGRKGDPGPSGPPGPRGPLGDPGPRGPPGLPGTAMKGDKGDRGERGPPGPGEGGIAPGEPGLPGLPGSPGPQGPVGPPGKKGEKGDSEDGAPGLPGQPGSPGEQGPRGPPGAIGPKGDRGFPGPLGEAGEKGERGPPGPAGSRGLPGVAGRPGAKGPEGPPGPTGRQGEKGEPGRPGDPAVVGPAVAGPKGEKGDVGPAGPRGATGVQGERGPPGLVLPGDPGPKGDPGDRGPIGLTGRAGPPGDSGPPGEKGDPGRPGPPGPVGPRGRDGEVGEKGDEGPPGDPGLPGKAGERGLRGAPGVRGPVGEKGDQGDPGEDGRNGSPGSSGPKGDRGEPGPPGPPGRLVDTGPGAREKGEPGDRGQEGPRGPKGDPGLPGAPGERGIEGFRGPPGPQGDPGVRGPAGEKGDRGPPGLDGRSGLDGKPGAAGPSGPNGAAGKAGDPGRDGLPGLRGEQGLPGPSGPPGLPGKPGEDGKPGLNGKNGEPGDPGEDGRKGEKGDSGASGREGRDGPKGERGAPGILGPQGPPGLPGPVGPPGQGFPGVPGGTGPKGDRGETGSKGEQGLPGERGLRGEPGSVPNVDRLLETAGIKASALREIVETWDESSGSFLPVPERRRGPKGDSGEQGPPGKEGPIGFPGERGLKGDRGDPGPQGPPGLALGERGPPGPSGLAGEPGKPGIPGLPGRAGGVGEAGRPGERGERGEKGERGEQGRDGPPGLPGTPGPPGPPGPKVSVDEPGPGLSGEQGPPGLKGAKGEPGSNGDQGPKGDRGVPGIKGDRGEPGPRGQDGNPGLPGERGMAGPEGKPGLQGPRGPPGPVGGHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLTGPTGAVGLPGPPGPSGLVGPQGSPGLPGQVGETGKPGAPGRDGASGKDGDRGSPGVPGSPGLPGPVGPKGEPGPTGAPGQAVVGLPGAKGEKGAPGGLAGDLVGEPGAKGDRGLPGPRGEKGEAGRAGEPGDPGEDGQKGAPGPKGFKGDPGVGVPGSPGPPGPPGVKGDLGLPGLPGAPGVVGFPGQTGPRGEMGQPGPSGERGLAGPPGREGIPGPLGPPGPPGSVGPPGASGLKGDKGEDGRPGQEGPRGLTGPPGSRGERGEKGDVGSAGLKGDKGDSAVILGPPGPRGAKGDMGERGPRGLDGDKGPRGDNGDPGDKGSKGEPGDKGSAGLPGLRGLLGPQGQPGAAGIPGDPGSPGKDGVPGIRGEKGDVGFMGPRGLKGERGVKGACGLDGEKGDKGEAGPPGRPGLAGHKGEMGEPGVPGQSGAPGKEGLIGPKGDRGFDGQPGPKGDQGEKGERGTPGIGGFPGPSGNDGSAGPPGPPGSVGPRGPEGLQGQKGERGPPGERVVGAPGVPGAPGERGEQGRPGPAGPRGEKGEAALTEDDIRGFVRQEMSQHCACQGQFIASGSRPLPSYAADTAGSQLHAVPVLRVSHAEEEERVPPEDDEYSEYSEYSVEEYQDPEAPWDSDDPCSLPLDEGSCTAYTLRWYHRAVTGSTEACHPFVYGGCGGNANRFGTREACERRCPPRVVQSQGTGTAQD

Target ORF information:

RefSeq Version	XM_017005688.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005688.1

ORF Insert Sequence:

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGTCA GCCAGGCCCT 
AGTGGAGAGC GGGGTCTGGC AGGCCCCCCA GGGAGAGAAG GAATCCCAGG ACCCCTGGGG 
CCACCTGGAC CACCGGGGTC AGTGGGACCA CCTGGGGCCT CTGGACTCAA AGGAGACAAG 
GGTGAAGATG GCCGCCCCGG CCAGGAGGGA CCCCGAGGAC TCACGGGGCC CCCTGGCAGC 
AGGGGAGAGC GTGGGGAGAA GGGTGATGTT GGGAGTGCAG GACTAAAGGG TGACAAGGGA 
GACTCAGCTG TGATCCTGGG GCCTCCAGGC CCACGGGGTG CCAAGGGGGA CATGGGTGAA 
CGAGGGCCTC GGGGCTTGGA TGGTGACAAA GGACCTCGGG GAGACAATGG GGACCCTGGT 
GACAAGGGCA GCAAGGGAGA GCCTGGTGAC AAGGGCTCAG CCGGGTTGCC AGGACTGCGT 
GGACTCCTGG GACCCCAGGG TCAACCTGGT GCAGCAGGGA TCCCTGGTGA CCCGGGATCC 
CCAGGAAAGG ATGGAGTGCC TGGTATCCGA GGAGAAAAAG GAGATGTTGG CTTCATGGGT 
CCCCGGGGCC TCAAGGGTGA ACGGGGAGTG AAGGGAGCCT GTGGCCTTGA TGGAGAGAAG 
GGAGACAAGG GAGAAGCTGG TCCCCCAGGC CGCCCCGGGC TGGCAGGACA CAAAGGAGAG 
ATGGGGGAGC CTGGTGTGCC GGGCCAGTCG GGGGCCCCTG GCAAGGAGGG CCTGATCGGT 
CCCAAGGGTG ACCGAGGCTT TGACGGGCAG CCAGGCCCCA AGGGTGACCA GGGCGAGAAA 
GGGGAGCGGG GAACCCCAGG AATTGGGGGC TTCCCAGGCC CCAGTGGAAA TGATGGCTCT 
GCTGGTCCCC CAGGGCCACC TGGCAGTGTT GGTCCCAGAG GCCCCGAAGG ACTTCAGGGC 
CAGAAGGGTG AGCGAGGTCC CCCCGGAGAG AGAGTGGTGG GGGCTCCTGG GGTCCCTGGA 
GCTCCTGGCG AGAGAGGGGA GCAGGGGCGG CCAGGGCCTG CCGGTCCTCG AGGCGAGAAG 
GGAGAAGCTG CACTGACGGA GGATGACATC CGGGGCTTTG TGCGCCAAGA GATGAGTCAG 
CACTGTGCCT GCCAGGGCCA GTTCATCGCA TCTGGATCAC GACCCCTCCC TAGTTATGCT 
GCAGACACTG CCGGCTCCCA GCTCCATGCT GTGCCTGTGC TCCGCGTCTC TCATGCAGAG 
GAGGAAGAGC GGGTACCCCC TGAGGATGAT GAGTACTCTG AATACTCCGA GTATTCTGTG 
GAGGAGTACC AGGACCCTGA AGCTCCTTGG GATAGTGATG ACCCCTGTTC CCTGCCACTG 
GATGAGGGCT CCTGCACTGC CTACACCCTG CGCTGGTACC ATCGGGCTGT GACAGGCAGC 
ACAGAGGCCT GTCACCCTTT TGTCTATGGT GGCTGTGGAG GGAATGCCAA CCGTTTTGGG 
ACCCGTGAGG CCTGCGAGCG CCGCTGCCCA CCCCGGGTGG TCCAGAGCCA GGGGACAGGT 
ACTGCCCAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL7A1 ( XM_017005689.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861178.1 Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X6, mRNA.

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CloneID	OHu93794
Clone ID Related Accession (Same CDS sequence)	XM_017005689.1
Accession Version	XM_017005689.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(VII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGTCA GCCAGGCCCT 
AGTGGAGAGC GGGGTCTGGC AGGCCCCCCA GGGAGAGAAG GAATCCCAGG ACCCCTGGGG 
CCACCTGGAC CACCGGGGTC AGTGGGACCA CCTGGGGCCT CTGGACTCAA AGGAGACAAG 
GGAGACCCTG GAGTAGGGCT GCCTGGGCCC CGAGGCGAGC GTGGGGAGCC AGGCATCCGG 
GGTGAAGATG GCCGCCCCGG CCAGGAGGGA CCCCGAGGAC TCACGGGGCC CCCTGGCAGC 
AGGGGAGAGC GTGGGGAGAA GGGTGATGTT GGGAGTGCAG GACTAAAGGG TGACAAGGGA 
GACTCAGCTG TGATCCTGGG GCCTCCAGGC CCACGGGGTG CCAAGGGGGA CATGGGTGAA 
CGAGGGCCTC GGGGCTTGGA TGGTGACAAA GGACCTCGGG GAGACAATGG GGACCCTGGT 
GACAAGGGCA GCAAGGGAGA GCCTGGTGAC AAGGGCTCAG CCGGGTTGCC AGGACTGCGT 
GGACTCCTGG GACCCCAGGG TCAACCTGGT GCAGCAGGGA TCCCTGGTGA CCCGGGCGAT 
TCTCTTTGGT CCCTCACTGA CCTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861178.1
CDS	37..7866
Translation	MTLRLLVAALCAGILAEAPRVRAQHRERVTCTRLYAADIVFLLDGSSSIGRSNFREVRSFLEGLVLPFSGAASAQGVRFATVQYSDDPRTEFGLDALGSGGDVIRAIRELSYKGGNTRTGAAILHVADHVFLPQLARPGVPKVCILITDGKSQDLVDTAAQRLKGQGVKLFAVGIKNADPEELKRVASQPTSDFFFFVNDFSILRTLLPLVSRRVCTTAGGVPVTRPPDDSTSAPRDLVLSEPSSQSLRVQWTAASGPVTGYKVQYTPLTGLGQPLPSERQEVNVPAGETSVRLRGLRPLTEYQVTVIALYANSIGEAVSGTARTTALEGPELTIQNTTAHSLLVAWRSVPGATGYRVTWRVLSGGPTQQQELGPGQGSVLLRDLEPGTDYEVTVSTLFGRSVGPATSLMARTDASVEQTLRPVILGPTSILLSWNLVPEARGYRLEWRRETGLEPPQKVVLPSDVTRYQLDGLQPGTEYRLTLYTLLEGHEVATPATVVPTGPELPVSPVTDLQATELPGQRVRVSWSPVPGATQYRIIVRSTQGVERTLVLPGSQTAFDLDDVQAGLSYTVRVSARVGPREGSASVLTVRREPETPLAVPGLRVVVSDATRVRVAWGPVPGASGFRISWSTGSGPESSQTLPPDSTATDITGLQPGTTYQVAVSVLRGREEGPAAVIVARTDPLGPVRTVHVTQASSSSVTITWTRVPGATGYRVSWHSAHGPEKSQLVSGEATVAELDGLEPDTEYTVHVRAHVAGVDGPPASVVVRTAPEPVGRVSRLQILNASSDVLRITWVGVTGATAYRLAWGRSEGGPMRHQILPGNTDSAEIRGLEGGVSYSVRVTALVGDREGTPVSIVVTTPPEAPPALGTLHVVQRGEHSLRLRWEPVPRAQGFLLHWQPEGGQEQSRVLGPELSSYHLDGLEPATQYRVRLSVLGPAGEGPSAEVTARTESPRVPSIELRVVDTSIDSVTLAWTPVSRASSYILSWRPLRGPGQEVPGSPQTLPGISSSQRVTGLEPGVSYIFSLTPVLDGVRGPEASVTQTPVCPRGLADVVFLPHATQDNAHRAEATRRVLERLVLALGPLGPQAVQVGLLSYSHRPSPLFPLNGSHDLGIILQRIRDMPYMDPSGNNLGTAVVTAHRYMLAPDAPGRRQHVPGVMVLLVDEPLRGDIFSPIREAQASGLNVVMLGMAGADPEQLRRLAPGMDSVQTFFAVDDGPSLDQAVSGLATALCQASFTTQPRPEPCPVYCPKGQKGEPGEMGLRGQVGPPGDPGLPGRTGAPGPQGPPGSATAKGERGFPGADGRPGSPGRAGNPGTPGAPGLKGSPGLPGPRGDPGERGPRGPKGEPGAPGQVIGGEGPGLPGRKGDPGPSGPPGPRGPLGDPGPRGPPGLPGTAMKGDKGDRGERGPPGPGEGGIAPGEPGLPGLPGSPGPQGPVGPPGKKGEKGDSEDGAPGLPGQPGSPGEQGPRGPPGAIGPKGDRGFPGPLGEAGEKGERGPPGPAGSRGLPGVAGRPGAKGPEGPPGPTGRQGEKGEPGRPGDPAVVGPAVAGPKGEKGDVGPAGPRGATGVQGERGPPGLVLPGDPGPKGDPGDRGPIGLTGRAGPPGDSGPPGEKGDPGRPGPPGPVGPRGRDGEVGEKGDEGPPGDPGLPGKAGERGLRGAPGVRGPVGEKGDQGDPGEDGRNGSPGSSGPKGDRGEPGPPGPPGRLVDTGPGAREKGEPGDRGQEGPRGPKGDPGLPGAPGERGIEGFRGPPGPQGDPGVRGPAGEKGDRGPPGLDGRSGLDGKPGAAGPSGPNGAAGKAGDPGRDGLPGLRGEQGLPGPSGPPGLPGKPGEDGKPGLNGKNGEPGDPGEDGRKGEKGDSGASGREGRDGPKGERGAPGILGPQGPPGLPGPVGPPGQGFPGVPGGTGPKGDRGETGSKGEQGLPGERGLRGEPGSVPNVDRLLETAGIKASALREIVETWDESSGSFLPVPERRRGPKGDSGEQGPPGKEGPIGFPGERGLKGDRGDPGPQGPPGLALGERGPPGPSGLAGEPGKPGIPGLPGRAGGVGEAGRPGERGERGEKGERGEQGRDGPPGLPGTPGPPGPPGPKVSVDEPGPGLSGEQGPPGLKGAKGEPGSNGDQGPKGDRGVPGIKGDRGEPGPRGQDGNPGLPGERGMAGPEGKPGLQGPRGPPGPVGGHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLTGPTGAVGLPGPPGPSGLVGPQGSPGLPGQVGETGKPGAPGRDGASGKDGDRGSPGVPGSPGLPGPVGPKGEPGPTGAPGQAVVGLPGAKGEKGAPGGLAGDLVGEPGAKGDRGLPGPRGEKGEAGRAGEPGDPGEDGQKGAPGPKGFKGDPGVGVPGSPGPPGPPGVKGDLGLPGLPGAPGVVGFPGQTGPRGEMGQPGPSGERGLAGPPGREGIPGPLGPPGPPGSVGPPGASGLKGDKGDPGVGLPGPRGERGEPGIRGEDGRPGQEGPRGLTGPPGSRGERGEKGDVGSAGLKGDKGDSAVILGPPGPRGAKGDMGERGPRGLDGDKGPRGDNGDPGDKGSKGEPGDKGSAGLPGLRGLLGPQGQPGAAGIPGDPGDSLWSLTDLL

Target ORF information:

RefSeq Version	XM_017005689.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005689.1

ORF Insert Sequence:

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGTCA GCCAGGCCCT 
AGTGGAGAGC GGGGTCTGGC AGGCCCCCCA GGGAGAGAAG GAATCCCAGG ACCCCTGGGG 
CCACCTGGAC CACCGGGGTC AGTGGGACCA CCTGGGGCCT CTGGACTCAA AGGAGACAAG 
GGAGACCCTG GAGTAGGGCT GCCTGGGCCC CGAGGCGAGC GTGGGGAGCC AGGCATCCGG 
GGTGAAGATG GCCGCCCCGG CCAGGAGGGA CCCCGAGGAC TCACGGGGCC CCCTGGCAGC 
AGGGGAGAGC GTGGGGAGAA GGGTGATGTT GGGAGTGCAG GACTAAAGGG TGACAAGGGA 
GACTCAGCTG TGATCCTGGG GCCTCCAGGC CCACGGGGTG CCAAGGGGGA CATGGGTGAA 
CGAGGGCCTC GGGGCTTGGA TGGTGACAAA GGACCTCGGG GAGACAATGG GGACCCTGGT 
GACAAGGGCA GCAAGGGAGA GCCTGGTGAC AAGGGCTCAG CCGGGTTGCC AGGACTGCGT 
GGACTCCTGG GACCCCAGGG TCAACCTGGT GCAGCAGGGA TCCCTGGTGA CCCGGGCGAT 
TCTCTTTGGT CCCTCACTGA CCTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL7A1 ( XM_017005690.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861179.1 Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X8, mRNA.

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CloneID	OHu93795
Clone ID Related Accession (Same CDS sequence)	XM_017005690.1
Accession Version	XM_017005690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(VII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGTCA GCCAGGCCCT 
AGTGGAGAGC GGGGTCTGGC AGGCCCCCCA GGGAGAGAAG GAATCCCAGG ACCCCTGGGG 
CCACCTGGAC CACCGGGGTC AGTGGGACCA CCTGGGGCCT CTGGACTCAA AGGAGACAAG 
CCGAACTCCC TTCATCCCAG CTCTTGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861179.1
CDS	37..7449
Translation	MTLRLLVAALCAGILAEAPRVRAQHRERVTCTRLYAADIVFLLDGSSSIGRSNFREVRSFLEGLVLPFSGAASAQGVRFATVQYSDDPRTEFGLDALGSGGDVIRAIRELSYKGGNTRTGAAILHVADHVFLPQLARPGVPKVCILITDGKSQDLVDTAAQRLKGQGVKLFAVGIKNADPEELKRVASQPTSDFFFFVNDFSILRTLLPLVSRRVCTTAGGVPVTRPPDDSTSAPRDLVLSEPSSQSLRVQWTAASGPVTGYKVQYTPLTGLGQPLPSERQEVNVPAGETSVRLRGLRPLTEYQVTVIALYANSIGEAVSGTARTTALEGPELTIQNTTAHSLLVAWRSVPGATGYRVTWRVLSGGPTQQQELGPGQGSVLLRDLEPGTDYEVTVSTLFGRSVGPATSLMARTDASVEQTLRPVILGPTSILLSWNLVPEARGYRLEWRRETGLEPPQKVVLPSDVTRYQLDGLQPGTEYRLTLYTLLEGHEVATPATVVPTGPELPVSPVTDLQATELPGQRVRVSWSPVPGATQYRIIVRSTQGVERTLVLPGSQTAFDLDDVQAGLSYTVRVSARVGPREGSASVLTVRREPETPLAVPGLRVVVSDATRVRVAWGPVPGASGFRISWSTGSGPESSQTLPPDSTATDITGLQPGTTYQVAVSVLRGREEGPAAVIVARTDPLGPVRTVHVTQASSSSVTITWTRVPGATGYRVSWHSAHGPEKSQLVSGEATVAELDGLEPDTEYTVHVRAHVAGVDGPPASVVVRTAPEPVGRVSRLQILNASSDVLRITWVGVTGATAYRLAWGRSEGGPMRHQILPGNTDSAEIRGLEGGVSYSVRVTALVGDREGTPVSIVVTTPPEAPPALGTLHVVQRGEHSLRLRWEPVPRAQGFLLHWQPEGGQEQSRVLGPELSSYHLDGLEPATQYRVRLSVLGPAGEGPSAEVTARTESPRVPSIELRVVDTSIDSVTLAWTPVSRASSYILSWRPLRGPGQEVPGSPQTLPGISSSQRVTGLEPGVSYIFSLTPVLDGVRGPEASVTQTPVCPRGLADVVFLPHATQDNAHRAEATRRVLERLVLALGPLGPQAVQVGLLSYSHRPSPLFPLNGSHDLGIILQRIRDMPYMDPSGNNLGTAVVTAHRYMLAPDAPGRRQHVPGVMVLLVDEPLRGDIFSPIREAQASGLNVVMLGMAGADPEQLRRLAPGMDSVQTFFAVDDGPSLDQAVSGLATALCQASFTTQPRPEPCPVYCPKGQKGEPGEMGLRGQVGPPGDPGLPGRTGAPGPQGPPGSATAKGERGFPGADGRPGSPGRAGNPGTPGAPGLKGSPGLPGPRGDPGERGPRGPKGEPGAPGQVIGGEGPGLPGRKGDPGPSGPPGPRGPLGDPGPRGPPGLPGTAMKGDKGDRGERGPPGPGEGGIAPGEPGLPGLPGSPGPQGPVGPPGKKGEKGDSEDGAPGLPGQPGSPGEQGPRGPPGAIGPKGDRGFPGPLGEAGEKGERGPPGPAGSRGLPGVAGRPGAKGPEGPPGPTGRQGEKGEPGRPGDPAVVGPAVAGPKGEKGDVGPAGPRGATGVQGERGPPGLVLPGDPGPKGDPGDRGPIGLTGRAGPPGDSGPPGEKGDPGRPGPPGPVGPRGRDGEVGEKGDEGPPGDPGLPGKAGERGLRGAPGVRGPVGEKGDQGDPGEDGRNGSPGSSGPKGDRGEPGPPGPPGRLVDTGPGAREKGEPGDRGQEGPRGPKGDPGLPGAPGERGIEGFRGPPGPQGDPGVRGPAGEKGDRGPPGLDGRSGLDGKPGAAGPSGPNGAAGKAGDPGRDGLPGLRGEQGLPGPSGPPGLPGKPGEDGKPGLNGKNGEPGDPGEDGRKGEKGDSGASGREGRDGPKGERGAPGILGPQGPPGLPGPVGPPGQGFPGVPGGTGPKGDRGETGSKGEQGLPGERGLRGEPGSVPNVDRLLETAGIKASALREIVETWDESSGSFLPVPERRRGPKGDSGEQGPPGKEGPIGFPGERGLKGDRGDPGPQGPPGLALGERGPPGPSGLAGEPGKPGIPGLPGRAGGVGEAGRPGERGERGEKGERGEQGRDGPPGLPGTPGPPGPPGPKVSVDEPGPGLSGEQGPPGLKGAKGEPGSNGDQGPKGDRGVPGIKGDRGEPGPRGQDGNPGLPGERGMAGPEGKPGLQGPRGPPGPVGGHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLTGPTGAVGLPGPPGPSGLVGPQGSPGLPGQVGETGKPGAPGRDGASGKDGDRGSPGVPGSPGLPGPVGPKGEPGPTGAPGQAVVGLPGAKGEKGAPGGLAGDLVGEPGAKGDRGLPGPRGEKGEAGRAGEPGDPGEDGQKGAPGPKGFKGDPGVGVPGSPGPPGPPGVKGDLGLPGLPGAPGVVGFPGQTGPRGEMGQPGPSGERGLAGPPGREGIPGPLGPPGPPGSVGPPGASGLKGDKPNSLHPSSCL

Target ORF information:

RefSeq Version	XM_017005690.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005690.1

ORF Insert Sequence:

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGTCA GCCAGGCCCT 
AGTGGAGAGC GGGGTCTGGC AGGCCCCCCA GGGAGAGAAG GAATCCCAGG ACCCCTGGGG 
CCACCTGGAC CACCGGGGTC AGTGGGACCA CCTGGGGCCT CTGGACTCAA AGGAGACAAG 
CCGAACTCCC TTCATCCCAG CTCTTGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL7A1 ( XM_017005691.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861180.1 Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X9, mRNA.

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CloneID	OHu93796
Clone ID Related Accession (Same CDS sequence)	XM_017005691.1
Accession Version	XM_017005691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(VII) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGGTC TGGCAGGCCC 
CCCAGGGAGA GAAGGAATCC CAGGACCCCT GGGGCCACCT GGACCACCGG GGTCAGTGGG 
ACCACCTGGG GCCTCTGGAC TCAAAGGAGA CAAGCCGAAC TCCCTTCATC CCAGCTCTTG 
CCTCTGATTT CCAACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861180.1
CDS	37..7437
Translation	MTLRLLVAALCAGILAEAPRVRAQHRERVTCTRLYAADIVFLLDGSSSIGRSNFREVRSFLEGLVLPFSGAASAQGVRFATVQYSDDPRTEFGLDALGSGGDVIRAIRELSYKGGNTRTGAAILHVADHVFLPQLARPGVPKVCILITDGKSQDLVDTAAQRLKGQGVKLFAVGIKNADPEELKRVASQPTSDFFFFVNDFSILRTLLPLVSRRVCTTAGGVPVTRPPDDSTSAPRDLVLSEPSSQSLRVQWTAASGPVTGYKVQYTPLTGLGQPLPSERQEVNVPAGETSVRLRGLRPLTEYQVTVIALYANSIGEAVSGTARTTALEGPELTIQNTTAHSLLVAWRSVPGATGYRVTWRVLSGGPTQQQELGPGQGSVLLRDLEPGTDYEVTVSTLFGRSVGPATSLMARTDASVEQTLRPVILGPTSILLSWNLVPEARGYRLEWRRETGLEPPQKVVLPSDVTRYQLDGLQPGTEYRLTLYTLLEGHEVATPATVVPTGPELPVSPVTDLQATELPGQRVRVSWSPVPGATQYRIIVRSTQGVERTLVLPGSQTAFDLDDVQAGLSYTVRVSARVGPREGSASVLTVRREPETPLAVPGLRVVVSDATRVRVAWGPVPGASGFRISWSTGSGPESSQTLPPDSTATDITGLQPGTTYQVAVSVLRGREEGPAAVIVARTDPLGPVRTVHVTQASSSSVTITWTRVPGATGYRVSWHSAHGPEKSQLVSGEATVAELDGLEPDTEYTVHVRAHVAGVDGPPASVVVRTAPEPVGRVSRLQILNASSDVLRITWVGVTGATAYRLAWGRSEGGPMRHQILPGNTDSAEIRGLEGGVSYSVRVTALVGDREGTPVSIVVTTPPEAPPALGTLHVVQRGEHSLRLRWEPVPRAQGFLLHWQPEGGQEQSRVLGPELSSYHLDGLEPATQYRVRLSVLGPAGEGPSAEVTARTESPRVPSIELRVVDTSIDSVTLAWTPVSRASSYILSWRPLRGPGQEVPGSPQTLPGISSSQRVTGLEPGVSYIFSLTPVLDGVRGPEASVTQTPVCPRGLADVVFLPHATQDNAHRAEATRRVLERLVLALGPLGPQAVQVGLLSYSHRPSPLFPLNGSHDLGIILQRIRDMPYMDPSGNNLGTAVVTAHRYMLAPDAPGRRQHVPGVMVLLVDEPLRGDIFSPIREAQASGLNVVMLGMAGADPEQLRRLAPGMDSVQTFFAVDDGPSLDQAVSGLATALCQASFTTQPRPEPCPVYCPKGQKGEPGEMGLRGQVGPPGDPGLPGRTGAPGPQGPPGSATAKGERGFPGADGRPGSPGRAGNPGTPGAPGLKGSPGLPGPRGDPGERGPRGPKGEPGAPGQVIGGEGPGLPGRKGDPGPSGPPGPRGPLGDPGPRGPPGLPGTAMKGDKGDRGERGPPGPGEGGIAPGEPGLPGLPGSPGPQGPVGPPGKKGEKGDSEDGAPGLPGQPGSPGEQGPRGPPGAIGPKGDRGFPGPLGEAGEKGERGPPGPAGSRGLPGVAGRPGAKGPEGPPGPTGRQGEKGEPGRPGDPAVVGPAVAGPKGEKGDVGPAGPRGATGVQGERGPPGLVLPGDPGPKGDPGDRGPIGLTGRAGPPGDSGPPGEKGDPGRPGPPGPVGPRGRDGEVGEKGDEGPPGDPGLPGKAGERGLRGAPGVRGPVGEKGDQGDPGEDGRNGSPGSSGPKGDRGEPGPPGPPGRLVDTGPGAREKGEPGDRGQEGPRGPKGDPGLPGAPGERGIEGFRGPPGPQGDPGVRGPAGEKGDRGPPGLDGRSGLDGKPGAAGPSGPNGAAGKAGDPGRDGLPGLRGEQGLPGPSGPPGLPGKPGEDGKPGLNGKNGEPGDPGEDGRKGEKGDSGASGREGRDGPKGERGAPGILGPQGPPGLPGPVGPPGQGFPGVPGGTGPKGDRGETGSKGEQGLPGERGLRGEPGSVPNVDRLLETAGIKASALREIVETWDESSGSFLPVPERRRGPKGDSGEQGPPGKEGPIGFPGERGLKGDRGDPGPQGPPGLALGERGPPGPSGLAGEPGKPGIPGLPGRAGGVGEAGRPGERGERGEKGERGEQGRDGPPGLPGTPGPPGPPGPKVSVDEPGPGLSGEQGPPGLKGAKGEPGSNGDQGPKGDRGVPGIKGDRGEPGPRGQDGNPGLPGERGMAGPEGKPGLQGPRGPPGPVGGHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLTGPTGAVGLPGPPGPSGLVGPQGSPGLPGQVGETGKPGAPGRDGASGKDGDRGSPGVPGSPGLPGPVGPKGEPGPTGAPGQAVVGLPGAKGEKGAPGGLAGDLVGEPGAKGDRGLPGPRGEKGEAGRAGEPGDPGEDGQKGAPGPKGFKGDPGVGVPGSPGPPGPPGVKGDLGLPGLPGAPGVVGFPGQTGPRGEMGSGRPPRERRNPRTPGATWTTGVSGTTWGLWTQRRQAELPSSQLLPLISNL

Target ORF information:

RefSeq Version	XM_017005691.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005691.1

ORF Insert Sequence:

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGGTC TGGCAGGCCC 
CCCAGGGAGA GAAGGAATCC CAGGACCCCT GGGGCCACCT GGACCACCGG GGTCAGTGGG 
ACCACCTGGG GCCTCTGGAC TCAAAGGAGA CAAGCCGAAC TCCCTTCATC CCAGCTCTTG 
CCTCTGATTT CCAACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL7A1 ( XM_017005692.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861181.1 Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X10, mRNA.

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CloneID	OHu93797
Clone ID Related Accession (Same CDS sequence)	XM_017005692.1
Accession Version	XM_017005692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(VII) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGGTC TGGCAGGCCC 
CCCAGGGAGA GAAGGAATCC CAGGACCCCT GGGGCCACCT GGACCACCGG GGTCAGTGGG 
ACCACCTGGG GCCTCTGGAC TCAAAGGAGA CAAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861181.1
CDS	37..7395
Translation	MTLRLLVAALCAGILAEAPRVRAQHRERVTCTRLYAADIVFLLDGSSSIGRSNFREVRSFLEGLVLPFSGAASAQGVRFATVQYSDDPRTEFGLDALGSGGDVIRAIRELSYKGGNTRTGAAILHVADHVFLPQLARPGVPKVCILITDGKSQDLVDTAAQRLKGQGVKLFAVGIKNADPEELKRVASQPTSDFFFFVNDFSILRTLLPLVSRRVCTTAGGVPVTRPPDDSTSAPRDLVLSEPSSQSLRVQWTAASGPVTGYKVQYTPLTGLGQPLPSERQEVNVPAGETSVRLRGLRPLTEYQVTVIALYANSIGEAVSGTARTTALEGPELTIQNTTAHSLLVAWRSVPGATGYRVTWRVLSGGPTQQQELGPGQGSVLLRDLEPGTDYEVTVSTLFGRSVGPATSLMARTDASVEQTLRPVILGPTSILLSWNLVPEARGYRLEWRRETGLEPPQKVVLPSDVTRYQLDGLQPGTEYRLTLYTLLEGHEVATPATVVPTGPELPVSPVTDLQATELPGQRVRVSWSPVPGATQYRIIVRSTQGVERTLVLPGSQTAFDLDDVQAGLSYTVRVSARVGPREGSASVLTVRREPETPLAVPGLRVVVSDATRVRVAWGPVPGASGFRISWSTGSGPESSQTLPPDSTATDITGLQPGTTYQVAVSVLRGREEGPAAVIVARTDPLGPVRTVHVTQASSSSVTITWTRVPGATGYRVSWHSAHGPEKSQLVSGEATVAELDGLEPDTEYTVHVRAHVAGVDGPPASVVVRTAPEPVGRVSRLQILNASSDVLRITWVGVTGATAYRLAWGRSEGGPMRHQILPGNTDSAEIRGLEGGVSYSVRVTALVGDREGTPVSIVVTTPPEAPPALGTLHVVQRGEHSLRLRWEPVPRAQGFLLHWQPEGGQEQSRVLGPELSSYHLDGLEPATQYRVRLSVLGPAGEGPSAEVTARTESPRVPSIELRVVDTSIDSVTLAWTPVSRASSYILSWRPLRGPGQEVPGSPQTLPGISSSQRVTGLEPGVSYIFSLTPVLDGVRGPEASVTQTPVCPRGLADVVFLPHATQDNAHRAEATRRVLERLVLALGPLGPQAVQVGLLSYSHRPSPLFPLNGSHDLGIILQRIRDMPYMDPSGNNLGTAVVTAHRYMLAPDAPGRRQHVPGVMVLLVDEPLRGDIFSPIREAQASGLNVVMLGMAGADPEQLRRLAPGMDSVQTFFAVDDGPSLDQAVSGLATALCQASFTTQPRPEPCPVYCPKGQKGEPGEMGLRGQVGPPGDPGLPGRTGAPGPQGPPGSATAKGERGFPGADGRPGSPGRAGNPGTPGAPGLKGSPGLPGPRGDPGERGPRGPKGEPGAPGQVIGGEGPGLPGRKGDPGPSGPPGPRGPLGDPGPRGPPGLPGTAMKGDKGDRGERGPPGPGEGGIAPGEPGLPGLPGSPGPQGPVGPPGKKGEKGDSEDGAPGLPGQPGSPGEQGPRGPPGAIGPKGDRGFPGPLGEAGEKGERGPPGPAGSRGLPGVAGRPGAKGPEGPPGPTGRQGEKGEPGRPGDPAVVGPAVAGPKGEKGDVGPAGPRGATGVQGERGPPGLVLPGDPGPKGDPGDRGPIGLTGRAGPPGDSGPPGEKGDPGRPGPPGPVGPRGRDGEVGEKGDEGPPGDPGLPGKAGERGLRGAPGVRGPVGEKGDQGDPGEDGRNGSPGSSGPKGDRGEPGPPGPPGRLVDTGPGAREKGEPGDRGQEGPRGPKGDPGLPGAPGERGIEGFRGPPGPQGDPGVRGPAGEKGDRGPPGLDGRSGLDGKPGAAGPSGPNGAAGKAGDPGRDGLPGLRGEQGLPGPSGPPGLPGKPGEDGKPGLNGKNGEPGDPGEDGRKGEKGDSGASGREGRDGPKGERGAPGILGPQGPPGLPGPVGPPGQGFPGVPGGTGPKGDRGETGSKGEQGLPGERGLRGEPGSVPNVDRLLETAGIKASALREIVETWDESSGSFLPVPERRRGPKGDSGEQGPPGKEGPIGFPGERGLKGDRGDPGPQGPPGLALGERGPPGPSGLAGEPGKPGIPGLPGRAGGVGEAGRPGERGERGEKGERGEQGRDGPPGLPGTPGPPGPPGPKVSVDEPGPGLSGEQGPPGLKGAKGEPGSNGDQGPKGDRGVPGIKGDRGEPGPRGQDGNPGLPGERGMAGPEGKPGLQGPRGPPGPVGGHGDPGPPGAPGLAGPAGPQGPSGLKGEPGETGPPGRGLTGPTGAVGLPGPPGPSGLVGPQGSPGLPGQVGETGKPGAPGRDGASGKDGDRGSPGVPGSPGLPGPVGPKGEPGPTGAPGQAVVGLPGAKGEKGAPGGLAGDLVGEPGAKGDRGLPGPRGEKGEAGRAGEPGDPGEDGQKGAPGPKGFKGDPGVGVPGSPGPPGPPGVKGDLGLPGLPGAPGVVGFPGQTGPRGEMGSGRPPRERRNPRTPGATWTTGVSGTTWGLWTQRRQG

Target ORF information:

RefSeq Version	XM_017005692.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type VII alpha 1 chain (COL7A1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005692.1

ORF Insert Sequence:

ATGACGCTGC GGCTTCTGGT GGCCGCGCTC TGCGCCGGGA TCCTGGCAGA GGCGCCCCGA 
GTGCGAGCCC AGCACAGGGA GAGAGTGACC TGCACGCGCC TTTACGCCGC TGACATTGTG 
TTCTTACTGG ATGGCTCCTC ATCCATTGGC CGCAGCAATT TCCGCGAGGT CCGCAGCTTT 
CTCGAAGGGC TGGTGCTGCC TTTCTCTGGA GCAGCCAGTG CACAGGGTGT GCGCTTTGCC 
ACAGTGCAGT ACAGCGATGA CCCACGGACA GAGTTCGGCC TGGATGCACT TGGCTCTGGG 
GGTGATGTGA TCCGCGCCAT CCGTGAGCTT AGCTACAAGG GGGGCAACAC TCGCACAGGG 
GCTGCAATTC TCCATGTGGC TGACCATGTC TTCCTGCCCC AGCTGGCCCG ACCTGGTGTC 
CCCAAGGTCT GCATCCTGAT CACAGACGGG AAGTCCCAGG ACCTGGTGGA CACAGCTGCC 
CAAAGGCTGA AGGGGCAGGG GGTCAAGCTA TTTGCTGTGG GGATCAAGAA TGCTGACCCT 
GAGGAGCTGA AGCGAGTTGC CTCACAGCCC ACCAGTGACT TCTTCTTCTT CGTCAATGAC 
TTCAGCATCT TGAGGACACT ACTGCCCCTC GTTTCCCGGA GAGTGTGCAC GACTGCTGGT 
GGCGTGCCTG TGACCCGACC TCCGGATGAC TCGACCTCTG CTCCACGAGA CCTGGTGCTG 
TCTGAGCCAA GCAGCCAATC CTTGAGAGTA CAGTGGACAG CGGCCAGTGG CCCTGTGACT 
GGCTACAAGG TCCAGTACAC TCCTCTGACG GGGCTGGGAC AGCCACTGCC GAGTGAGCGG 
CAGGAGGTGA ACGTCCCAGC TGGTGAGACC AGTGTGCGGC TGCGGGGTCT CCGGCCACTG 
ACCGAGTACC AAGTGACTGT GATTGCCCTC TACGCCAACA GCATCGGGGA GGCTGTGAGC 
GGGACAGCTC GGACCACTGC CCTAGAAGGG CCGGAACTGA CCATCCAGAA TACCACAGCC 
CACAGCCTCC TGGTGGCCTG GCGGAGTGTG CCAGGTGCCA CTGGCTACCG TGTGACATGG 
CGGGTCCTCA GTGGTGGGCC CACACAGCAG CAGGAGCTGG GCCCTGGGCA GGGTTCAGTG 
TTGCTGCGTG ACTTGGAGCC TGGCACGGAC TATGAGGTGA CCGTGAGCAC CCTATTTGGC 
CGCAGTGTGG GGCCCGCCAC TTCCCTGATG GCTCGCACTG ACGCTTCTGT TGAGCAGACC 
CTGCGCCCGG TCATCCTGGG CCCCACATCC ATCCTCCTTT CCTGGAACTT GGTGCCTGAG 
GCCCGTGGCT ACCGGTTGGA ATGGCGGCGT GAGACTGGCT TGGAGCCACC GCAGAAGGTG 
GTACTGCCCT CTGATGTGAC CCGCTACCAG TTGGATGGGC TGCAGCCGGG CACTGAGTAC 
CGCCTCACAC TCTACACTCT GCTGGAGGGC CACGAGGTGG CCACCCCTGC AACCGTGGTT 
CCCACTGGAC CAGAGCTGCC TGTGAGCCCT GTAACAGACC TGCAAGCCAC CGAGCTGCCC 
GGGCAGCGGG TGCGAGTGTC CTGGAGCCCA GTCCCTGGTG CCACCCAGTA CCGCATCATT 
GTGCGCAGCA CCCAGGGGGT TGAGCGGACC CTGGTGCTTC CTGGGAGTCA GACAGCATTC 
GACTTGGATG ACGTTCAGGC TGGGCTTAGC TACACTGTGC GGGTGTCTGC TCGAGTGGGT 
CCCCGTGAGG GCAGTGCCAG TGTCCTCACT GTCCGCCGGG AGCCGGAAAC TCCACTTGCT 
GTTCCAGGGC TGCGGGTTGT GGTGTCAGAT GCAACGCGAG TGAGGGTGGC CTGGGGACCC 
GTCCCTGGAG CCAGTGGATT TCGGATTAGC TGGAGCACAG GCAGTGGTCC GGAGTCCAGC 
CAGACACTGC CCCCAGACTC TACTGCCACA GACATCACAG GGCTGCAGCC TGGAACCACC 
TACCAGGTGG CTGTGTCGGT ACTGCGAGGC AGAGAGGAGG GCCCTGCTGC AGTCATCGTG 
GCTCGAACGG ACCCACTGGG CCCAGTGAGG ACGGTCCATG TGACTCAGGC CAGCAGCTCA 
TCTGTCACCA TTACCTGGAC CAGGGTTCCT GGCGCCACAG GATACAGGGT TTCCTGGCAC 
TCAGCCCACG GCCCAGAGAA ATCCCAGTTG GTTTCTGGGG AGGCCACGGT GGCTGAGCTG 
GATGGACTGG AGCCAGATAC TGAGTATACG GTGCATGTGA GGGCCCATGT GGCTGGCGTG 
GATGGGCCCC CTGCCTCTGT GGTTGTGAGG ACTGCCCCTG AGCCTGTGGG TCGTGTGTCG 
AGGCTGCAGA TCCTCAATGC TTCCAGCGAC GTTCTACGGA TCACCTGGGT AGGGGTCACT 
GGAGCCACAG CTTACAGACT GGCCTGGGGC CGGAGTGAAG GCGGCCCCAT GAGGCACCAG 
ATACTCCCAG GAAACACAGA CTCTGCAGAG ATCCGGGGTC TCGAAGGTGG AGTCAGCTAC 
TCAGTGCGAG TGACTGCACT TGTCGGGGAC CGCGAGGGCA CACCTGTCTC CATTGTTGTC 
ACTACGCCGC CTGAGGCTCC GCCAGCCCTG GGGACGCTTC ACGTGGTGCA GCGCGGGGAG 
CACTCGCTGA GGCTGCGCTG GGAGCCGGTG CCCAGAGCGC AGGGCTTCCT TCTGCACTGG 
CAACCTGAGG GTGGCCAGGA ACAGTCCCGG GTCCTGGGGC CCGAGCTCAG CAGCTATCAC 
CTGGACGGGC TGGAGCCAGC GACACAGTAC CGCGTGAGGC TGAGTGTCCT AGGGCCAGCT 
GGAGAAGGGC CCTCTGCAGA GGTGACTGCG CGCACTGAGT CACCTCGTGT TCCAAGCATT 
GAACTACGTG TGGTGGACAC CTCGATCGAC TCGGTGACTT TGGCCTGGAC TCCAGTGTCC 
AGGGCATCCA GCTACATCCT ATCCTGGCGG CCACTCAGAG GCCCTGGCCA GGAAGTGCCT 
GGGTCCCCGC AGACACTTCC AGGGATCTCA AGCTCCCAGC GGGTGACAGG GCTAGAGCCT 
GGCGTCTCTT ACATCTTCTC CCTGACGCCT GTCCTGGATG GTGTGCGGGG TCCTGAGGCA 
TCTGTCACAC AGACGCCAGT GTGCCCCCGT GGCCTGGCGG ATGTGGTGTT CCTACCACAT 
GCCACTCAAG ACAATGCTCA CCGTGCGGAG GCTACGAGGA GGGTCCTGGA GCGTCTGGTG 
TTGGCACTTG GGCCTCTTGG GCCACAGGCA GTTCAGGTTG GCCTGCTGTC TTACAGTCAT 
CGGCCCTCCC CACTGTTCCC ACTGAATGGC TCCCATGACC TTGGCATTAT CTTGCAAAGG 
ATCCGTGACA TGCCCTACAT GGACCCAAGT GGGAACAACC TGGGCACAGC CGTGGTCACA 
GCTCACAGAT ACATGTTGGC ACCAGATGCT CCTGGGCGCC GCCAGCACGT ACCAGGGGTG 
ATGGTTCTGC TAGTGGATGA ACCCTTGAGA GGTGACATAT TCAGCCCCAT CCGTGAGGCC 
CAGGCTTCTG GGCTTAATGT GGTGATGTTG GGAATGGCTG GAGCGGACCC AGAGCAGCTG 
CGTCGCTTGG CGCCGGGTAT GGACTCTGTC CAGACCTTCT TCGCCGTGGA TGATGGGCCA 
AGCCTGGACC AGGCAGTCAG TGGTCTGGCC ACAGCCCTGT GTCAGGCATC CTTCACTACT 
CAGCCCCGGC CAGAGCCCTG CCCAGTGTAT TGTCCAAAGG GCCAGAAGGG GGAACCTGGA 
GAGATGGGCC TGAGAGGACA AGTTGGGCCT CCTGGCGACC CTGGCCTCCC GGGCAGGACC 
GGTGCTCCCG GCCCCCAGGG GCCCCCTGGA AGTGCCACTG CCAAGGGCGA GAGGGGCTTC 
CCTGGAGCAG ATGGGCGTCC AGGCAGCCCT GGCCGCGCCG GGAATCCTGG GACCCCTGGA 
GCCCCTGGCC TAAAGGGCTC TCCAGGGTTG CCTGGCCCTC GTGGGGACCC GGGAGAGCGA 
GGACCTCGAG GCCCAAAGGG GGAGCCGGGG GCTCCCGGAC AAGTCATCGG AGGTGAAGGA 
CCTGGGCTTC CTGGGCGGAA AGGGGACCCT GGACCATCGG GCCCCCCTGG ACCTCGTGGA 
CCACTGGGGG ACCCAGGACC CCGTGGCCCC CCAGGGCTTC CTGGAACAGC CATGAAGGGT 
GACAAAGGCG ATCGTGGGGA GCGGGGTCCC CCTGGACCAG GTGAAGGTGG CATTGCTCCT 
GGGGAGCCTG GGCTGCCGGG TCTTCCCGGA AGCCCTGGAC CCCAAGGCCC CGTTGGCCCC 
CCTGGAAAGA AAGGAGAAAA AGGTGACTCT GAGGATGGAG CTCCAGGCCT CCCAGGACAA 
CCTGGGTCTC CGGGTGAGCA GGGCCCACGG GGACCTCCTG GAGCTATTGG CCCCAAAGGT 
GACCGGGGCT TTCCAGGGCC CCTGGGTGAG GCTGGAGAGA AGGGCGAACG TGGACCCCCA 
GGCCCAGCGG GATCCCGGGG GCTGCCAGGG GTTGCTGGAC GTCCTGGAGC CAAGGGTCCT 
GAAGGGCCAC CAGGACCCAC TGGCCGCCAA GGAGAGAAGG GGGAGCCTGG TCGCCCTGGG 
GACCCTGCAG TGGTGGGACC TGCTGTTGCT GGACCCAAAG GAGAAAAGGG AGATGTGGGG 
CCCGCTGGGC CCAGAGGAGC TACCGGAGTC CAAGGGGAAC GGGGCCCACC CGGCTTGGTT 
CTTCCTGGAG ACCCTGGCCC CAAGGGAGAC CCTGGAGACC GGGGTCCCAT TGGCCTTACT 
GGCAGAGCAG GACCCCCAGG TGACTCAGGG CCTCCTGGAG AGAAGGGAGA CCCTGGGCGG 
CCTGGCCCCC CAGGACCTGT TGGCCCCCGA GGACGAGATG GTGAAGTTGG AGAGAAAGGT 
GACGAGGGTC CTCCGGGTGA CCCGGGTTTG CCTGGAAAAG CAGGCGAGCG TGGCCTTCGG 
GGGGCACCTG GAGTTCGGGG GCCTGTGGGT GAAAAGGGAG ACCAGGGAGA TCCTGGAGAG 
GATGGACGAA ATGGCAGCCC TGGATCATCT GGACCCAAGG GTGACCGTGG GGAGCCGGGT 
CCCCCAGGAC CCCCGGGACG GCTGGTAGAC ACAGGACCTG GAGCCAGAGA GAAGGGAGAG 
CCTGGGGACC GCGGACAAGA GGGTCCTCGA GGGCCCAAGG GTGATCCTGG CCTCCCTGGA 
GCCCCTGGGG AAAGGGGCAT TGAAGGGTTT CGGGGACCCC CAGGCCCACA GGGGGACCCA 
GGTGTCCGAG GCCCAGCAGG AGAAAAGGGT GACCGGGGTC CCCCTGGGCT GGATGGCCGG 
AGCGGACTGG ATGGGAAACC AGGAGCCGCT GGGCCCTCTG GGCCGAATGG TGCTGCAGGC 
AAAGCTGGGG ACCCAGGGAG AGACGGGCTT CCAGGCCTCC GTGGAGAACA GGGCCTCCCT 
GGCCCCTCTG GTCCCCCTGG ATTACCGGGA AAGCCAGGCG AGGATGGCAA ACCTGGCCTG 
AATGGAAAAA ACGGAGAACC TGGGGACCCT GGAGAAGACG GGAGGAAGGG AGAGAAAGGA 
GATTCAGGCG CCTCTGGGAG AGAAGGTCGT GATGGCCCCA AGGGTGAGCG TGGAGCTCCT 
GGTATCCTTG GACCCCAGGG GCCTCCAGGC CTCCCAGGGC CAGTGGGCCC TCCTGGCCAG 
GGTTTTCCTG GTGTCCCAGG AGGCACGGGC CCCAAGGGTG ACCGTGGGGA GACTGGATCC 
AAAGGGGAGC AGGGCCTCCC TGGAGAGCGT GGCCTGCGAG GAGAGCCTGG AAGTGTGCCG 
AATGTGGATC GGTTGCTGGA AACTGCTGGC ATCAAGGCAT CTGCCCTGCG GGAGATCGTG 
GAGACCTGGG ATGAGAGCTC TGGTAGCTTC CTGCCTGTGC CCGAACGGCG TCGAGGCCCC 
AAGGGGGACT CAGGCGAACA GGGCCCCCCA GGCAAGGAGG GCCCCATCGG CTTTCCTGGA 
GAACGCGGGC TGAAGGGCGA CCGTGGAGAC CCTGGCCCTC AGGGGCCACC TGGTCTGGCC 
CTTGGGGAGA GGGGCCCCCC CGGGCCTTCC GGCCTTGCCG GGGAGCCTGG AAAGCCTGGT 
ATTCCCGGGC TCCCAGGCAG GGCTGGGGGT GTGGGAGAGG CAGGAAGGCC AGGAGAGAGG 
GGAGAACGGG GAGAGAAAGG AGAACGTGGA GAACAGGGCA GAGATGGCCC TCCTGGACTC 
CCTGGAACCC CTGGGCCCCC CGGACCCCCT GGCCCCAAGG TGTCTGTGGA TGAGCCAGGT 
CCTGGACTCT CTGGAGAACA GGGACCCCCT GGACTCAAGG GTGCTAAGGG GGAGCCGGGC 
AGCAATGGTG ACCAAGGTCC CAAAGGAGAC AGGGGTGTGC CAGGCATCAA AGGAGACCGG 
GGAGAGCCTG GACCGAGGGG TCAGGACGGC AACCCGGGTC TACCAGGAGA GCGTGGTATG 
GCTGGGCCTG AAGGGAAGCC GGGTCTGCAG GGTCCAAGAG GCCCCCCTGG CCCAGTGGGT 
GGTCATGGAG ACCCTGGACC ACCTGGTGCC CCGGGTCTTG CTGGCCCTGC AGGACCCCAA 
GGACCTTCTG GCCTGAAGGG GGAGCCTGGA GAGACAGGAC CTCCAGGACG GGGCCTGACT 
GGACCTACTG GAGCTGTGGG ACTTCCTGGA CCCCCCGGCC CTTCAGGCCT TGTGGGTCCA 
CAGGGGTCTC CAGGTTTGCC TGGACAAGTG GGGGAGACAG GGAAGCCGGG AGCCCCAGGT 
CGAGATGGTG CCAGTGGAAA AGATGGAGAC AGAGGGAGCC CTGGTGTGCC AGGGTCACCA 
GGTCTGCCTG GCCCTGTCGG ACCTAAAGGA GAACCTGGCC CCACGGGGGC CCCTGGACAG 
GCTGTGGTCG GGCTCCCTGG AGCAAAGGGA GAGAAGGGAG CCCCTGGAGG CCTTGCTGGA 
GACCTGGTGG GTGAGCCGGG AGCCAAAGGT GACCGAGGAC TGCCAGGGCC GCGAGGCGAG 
AAGGGTGAAG CTGGCCGTGC AGGGGAGCCC GGAGACCCTG GGGAAGATGG TCAGAAAGGG 
GCTCCAGGAC CCAAAGGTTT CAAGGGTGAC CCAGGAGTCG GGGTCCCGGG CTCCCCTGGG 
CCTCCTGGCC CTCCAGGTGT GAAGGGAGAT CTGGGCCTCC CTGGCCTGCC CGGTGCTCCT 
GGTGTTGTTG GGTTCCCGGG TCAGACAGGC CCTCGAGGAG AGATGGGGTC TGGCAGGCCC 
CCCAGGGAGA GAAGGAATCC CAGGACCCCT GGGGCCACCT GGACCACCGG GGTCAGTGGG 
ACCACCTGGG GCCTCTGGAC TCAAAGGAGA CAAGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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COL7A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報