COL5A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL5A1
Entrez Gene ID	1289
Full Name	collagen type V alpha 1 chain
Synonyms	EDSC,EDSCL1
General protein information	Preferred Names collagen type V alpha 1 chain Names collagen alpha-1(V) chain collagen, type V, alpha 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.3
Summary	This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013].
Disorder MIM:	120215
Disorder Html:	Ehlers-Danlos syndrome, classic type, 130000 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017014266.1	XP_016869755.1	collagen alpha-1(V) chain isoform X1
NM_000093.5	NP_000084.3	collagen alpha-1(V) chain isoform 1 preproprotein
NM_000093.4	NP_000084.3	collagen alpha-1(V) chain isoform 1 preproprotein
XM_017014266.2	XP_016869755.1	collagen alpha-1(V) chain isoform X1
NM_001278074.1	NP_001265003.1	collagen alpha-1(V) chain isoform 2 preproprotein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col5a1	NP_604447.1
Homo sapiens (human)	COL5A1	NP_001265003.1
Mus musculus (house mouse)	Col5a1	NP_056549.2
Gallus gallus (chicken)	COL5A1	NP_990121.1
Xenopus tropicalis (tropical clawed frog)	col5a1	XP_004916702.1
Danio rerio (zebrafish)	col5a1	XP_002665924.2

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of tissue-specific and developmentally regulated alternative splicing of exon 64 in the COL5A1 gene.
Mitchell AL, Judis LM, Schwarze U, Vaynshtok PM, Drumm ML, Byers PH
Connective tissue research53(3)267-76(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both in cell lines and in mouse model, the extracellular matrix receptors including the integrin ( ITGA3 and ITGA2B), collagen ( COL5A1), and laminin ( LAMA5) were significantly inhibited by curcumin at messenger RNA and protein levels.
Title: Identification of curcumin-inhibited extracellular matrix receptors in non-small cell lung cancer A549 cells by RNA sequencing.

Data suggest that, compared with obese white women, obese black women exhibit higher expression of HIF1A (hypoxia inducible factor 1 alpha), COL5A1 (collagen Valpha1), and COL6A1 (collagen VIalpha1) in gluteal but not abdominal subcutaneous adipose tissue depots; up-regulation of expression of these proteins correlates with reduced insulin sensitivity in black women only.
Title: Hypoxia and extra-cellular matrix gene expression in adipose tissue associates with reduced insulin sensitivity in black South African women.

Variants of the COL5A1 gene seem to influence quadriceps muscle-tendon stiffness.
Title: Human COL5A1 polymorphisms and quadriceps muscle-tendon mechanical stiffness in vivo.

Gene expression levels of three randomly selected DEGs, VCAN, COL5A1 and KCNJ16, were examined using RT-PCR in 10 ATC samples.. angiogenesis was activated by the high expression of CTHRC1, VCAN and POSTN, providing necessary nutrition for tumor cells
Title: Elucidation of the molecular mechanisms of anaplastic thyroid carcinoma by integrated miRNA and mRNA analysis.

COL5A1 mutation is associated with Adrenocortical Adenomas.
Title: Genetic Landscape of Sporadic Unilateral Adrenocortical Adenomas Without PRKACA p.Leu206Arg Mutation.

The following COL5A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL5A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20536	NM_001278074.1 Latest version!	Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu22455	NM_000093.5 Latest version!	Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu98441	XM_017014266.2 Latest version!	Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu22455	NM_000093.4	Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu98441	XM_017014266.1	Homo sapiens collagen type V alpha 1 (COL5A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL5A1 ( NM_001278074.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265003.1 Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant 2, mRNA.

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CloneID	OHu20536
Clone ID Related Accession (Same CDS sequence)	NM_001278074.1
Accession Version	NM_001278074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	collagen alpha-1(V) chain isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591890.31, AB371583.1, CN399221.1, AB209864.1, CN399265.1, CN399198.1, BM051019.1, AI190929.1, CB152831.1, ES314363.1, BC032405.1, CN484933.1 and CA446007.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) contains an alternate in-frame exon in the 3' coding region compared to variant 1. It encodes isoform 2 which is of the same length but contains an alternate segment, compared to isoform 1. This variant is based on experimental data in PMID 22149965 and is supported by partial transcripts and RNA-seq data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 22149965 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGAGT AAAATGGCCC GCTGGCCCAA AGAGCAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGG TCCCTGCTCT CCTATGTGGA CGCCGAGGGC 
AACCCTGTGG GTGTGGTACA GATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACCACTGCTA CCAGTCAGTG GCCTGGCAGG ACGCAGCCAC GGGCAGCTAC 
GACAAGGCCC TCCGCTTCCT GGGCTCCAAC GACGAGGAGA TGTCCTATGA CAACAACCCC 
TACATCCGCG CCCTGGTGGA CGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTTCTG 
GAGATCGACA CCCCCAAAGT GGAGCAGGTG CCCATCGTGG ACATCATGTT CAATGACTTC 
GGTGAAGCGT CACAGAAATT TGGATTTGAA GTGGGGCCGG CTTGCTTCAT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265003.1
CDS	384..5900
Translation	MDVHTRWKARSALRPGAPLLPPLLLLLLWAPPPSRAAQPADLLKVLDFHNLPDGITKTTGFCATRRSSKGPDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQIGLELGRSPVFLYEDHTGKPGPEDYPLFRGINLSDGKWHRIALSVHKKNVTLILDCKKKTTKFLDRSDHPMIDINGIIVFGTRILDEEVFEGDIQQLLFVSDHRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYTEGDGEGETYYYEYPYYEDPEDLGKEPTPSKKPVEAAKETTEVPEELTPTPTEAAPMPETSEGAGKEEDVGIGDYDYVPSEDYYTPSPYDDLTYGEGEENPDQPTDPGAGAEIPTSTADTSNSSNPAPPPGEGADDLEGEFTEETIRNLDENYYDPYYDPTSSPSEIGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPPGTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPVGPPGSGGLKGEPGDVGPQGPRGVQGPPGPAGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGPPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGLAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGNEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEKGESGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGEQGITGPSGPIGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHPGPPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGNGENYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGSKMARWPKEQPSTWYSQYKRGSLLSYVDAEGNPVGVVQMTFLRLLSASAHQNVTYHCYQSVAWQDAATGSYDKALRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFMG

Target ORF information:

RefSeq Version	NM_001278074.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278074.1

ORF Insert Sequence:

ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGAGT AAAATGGCCC GCTGGCCCAA AGAGCAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGG TCCCTGCTCT CCTATGTGGA CGCCGAGGGC 
AACCCTGTGG GTGTGGTACA GATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACCACTGCTA CCAGTCAGTG GCCTGGCAGG ACGCAGCCAC GGGCAGCTAC 
GACAAGGCCC TCCGCTTCCT GGGCTCCAAC GACGAGGAGA TGTCCTATGA CAACAACCCC 
TACATCCGCG CCCTGGTGGA CGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTTCTG 
GAGATCGACA CCCCCAAAGT GGAGCAGGTG CCCATCGTGG ACATCATGTT CAATGACTTC 
GGTGAAGCGT CACAGAAATT TGGATTTGAA GTGGGGCCGG CTTGCTTCAT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A1 ( NM_000093.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000084.3 Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant 1, mRNA.

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CloneID	OHu22455
Clone ID Related Accession (Same CDS sequence)	NM_000093.5 , NM_000093.4
Accession Version	NM_000093.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	collagen alpha-1(V) chain isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591890.31, AB371583.1, CN399221.1, AB209864.1, CN399265.1, BM051019.1, AI190929.1, CB152831.1, ES314363.1, BC032405.1, CN484933.1 and CA446007.1. On Aug 31, 2019 this sequence version replaced NM_000093.4. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB371583.1, D90279.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGGCC AGAATCACTT CTTGGCCCAA AGAAAACCCG 
GGCTCCTGGT TCAGTGAATT CAAGCGTGGG AAACTGCTCT CCTATGTGGA CGCCGAGGGC 
AACCCTGTGG GTGTGGTACA GATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACCACTGCTA CCAGTCAGTG GCCTGGCAGG ACGCAGCCAC GGGCAGCTAC 
GACAAGGCCC TCCGCTTCCT GGGCTCCAAC GACGAGGAGA TGTCCTATGA CAACAACCCC 
TACATCCGCG CCCTGGTGGA CGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTTCTG 
GAGATCGACA CCCCCAAAGT GGAGCAGGTG CCCATCGTGG ACATCATGTT CAATGACTTC 
GGTGAAGCGT CACAGAAATT TGGATTTGAA GTGGGGCCGG CTTGCTTCAT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000084.3
CDS	386..5902
Translation	MDVHTRWKARSALRPGAPLLPPLLLLLLWAPPPSRAAQPADLLKVLDFHNLPDGITKTTGFCATRRSSKGPDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQIGLELGRSPVFLYEDHTGKPGPEDYPLFRGINLSDGKWHRIALSVHKKNVTLILDCKKKTTKFLDRSDHPMIDINGIIVFGTRILDEEVFEGDIQQLLFVSDHRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYTEGDGEGETYYYEYPYYEDPEDLGKEPTPSKKPVEAAKETTEVPEELTPTPTEAAPMPETSEGAGKEEDVGIGDYDYVPSEDYYTPSPYDDLTYGEGEENPDQPTDPGAGAEIPTSTADTSNSSNPAPPPGEGADDLEGEFTEETIRNLDENYYDPYYDPTSSPSEIGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPPGTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPVGPPGSGGLKGEPGDVGPQGPRGVQGPPGPAGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGPPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGLAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGNEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEKGESGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGEQGITGPSGPIGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHPGPPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGNGENYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGARITSWPKENPGSWFSEFKRGKLLSYVDAEGNPVGVVQMTFLRLLSASAHQNVTYHCYQSVAWQDAATGSYDKALRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFMG

Target ORF information:

RefSeq Version	NM_000093.5
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000093.5

ORF Insert Sequence:

ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGGCC AGAATCACTT CTTGGCCCAA AGAAAACCCG 
GGCTCCTGGT TCAGTGAATT CAAGCGTGGG AAACTGCTCT CCTATGTGGA CGCCGAGGGC 
AACCCTGTGG GTGTGGTACA GATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACCACTGCTA CCAGTCAGTG GCCTGGCAGG ACGCAGCCAC GGGCAGCTAC 
GACAAGGCCC TCCGCTTCCT GGGCTCCAAC GACGAGGAGA TGTCCTATGA CAACAACCCC 
TACATCCGCG CCCTGGTGGA CGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTTCTG 
GAGATCGACA CCCCCAAAGT GGAGCAGGTG CCCATCGTGG ACATCATGTT CAATGACTTC 
GGTGAAGCGT CACAGAAATT TGGATTTGAA GTGGGGCCGG CTTGCTTCAT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A1 ( NM_000093.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000084.3 Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant 1, mRNA.

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CloneID	OHu22455
Clone ID Related Accession (Same CDS sequence)	NM_000093.5 , NM_000093.4
Accession Version	NM_000093.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-27
Organism	Homo sapiens(human)
Product	collagen alpha-1(V) chain isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL591890.31, AB371583.1, CN399221.1, AB209864.1, CN399265.1, BM051019.1, AI190929.1, CB152831.1, ES314363.1, BC032405.1, CN484933.1 and CA446007.1. This sequence is a reference standard in the RefSeqGene project. On May 10, 2013 this sequence version replaced NM_000093.3. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB371583.1, D90279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGGCC AGAATCACTT CTTGGCCCAA AGAAAACCCG 
GGCTCCTGGT TCAGTGAATT CAAGCGTGGG AAACTGCTCT CCTATGTGGA CGCCGAGGGC 
AACCCTGTGG GTGTGGTACA GATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACCACTGCTA CCAGTCAGTG GCCTGGCAGG ACGCAGCCAC GGGCAGCTAC 
GACAAGGCCC TCCGCTTCCT GGGCTCCAAC GACGAGGAGA TGTCCTATGA CAACAACCCC 
TACATCCGCG CCCTGGTGGA CGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTTCTG 
GAGATCGACA CCCCCAAAGT GGAGCAGGTG CCCATCGTGG ACATCATGTT CAATGACTTC 
GGTGAAGCGT CACAGAAATT TGGATTTGAA GTGGGGCCGG CTTGCTTCAT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000084.3
CDS	384..5900
Translation	MDVHTRWKARSALRPGAPLLPPLLLLLLWAPPPSRAAQPADLLKVLDFHNLPDGITKTTGFCATRRSSKGPDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQIGLELGRSPVFLYEDHTGKPGPEDYPLFRGINLSDGKWHRIALSVHKKNVTLILDCKKKTTKFLDRSDHPMIDINGIIVFGTRILDEEVFEGDIQQLLFVSDHRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYTEGDGEGETYYYEYPYYEDPEDLGKEPTPSKKPVEAAKETTEVPEELTPTPTEAAPMPETSEGAGKEEDVGIGDYDYVPSEDYYTPSPYDDLTYGEGEENPDQPTDPGAGAEIPTSTADTSNSSNPAPPPGEGADDLEGEFTEETIRNLDENYYDPYYDPTSSPSEIGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPPGTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPVGPPGSGGLKGEPGDVGPQGPRGVQGPPGPAGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGPPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGLAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGNEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEKGESGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGEQGITGPSGPIGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHPGPPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGNGENYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGARITSWPKENPGSWFSEFKRGKLLSYVDAEGNPVGVVQMTFLRLLSASAHQNVTYHCYQSVAWQDAATGSYDKALRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDFGEASQKFGFEVGPACFMG

Target ORF information:

RefSeq Version	NM_000093.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000093.4

ORF Insert Sequence:

ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGGCC AGAATCACTT CTTGGCCCAA AGAAAACCCG 
GGCTCCTGGT TCAGTGAATT CAAGCGTGGG AAACTGCTCT CCTATGTGGA CGCCGAGGGC 
AACCCTGTGG GTGTGGTACA GATGACCTTC CTGCGGCTGC TGAGCGCCTC TGCCCACCAG 
AACGTCACCT ACCACTGCTA CCAGTCAGTG GCCTGGCAGG ACGCAGCCAC GGGCAGCTAC 
GACAAGGCCC TCCGCTTCCT GGGCTCCAAC GACGAGGAGA TGTCCTATGA CAACAACCCC 
TACATCCGCG CCCTGGTGGA CGGCTGTGCT ACCAAGAAAG GCTACCAGAA GACGGTTCTG 
GAGATCGACA CCCCCAAAGT GGAGCAGGTG CCCATCGTGG ACATCATGTT CAATGACTTC 
GGTGAAGCGT CACAGAAATT TGGATTTGAA GTGGGGCCGG CTTGCTTCAT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A1 ( XM_017014266.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869755.1 Homo sapiens collagen type V alpha 1 (COL5A1), transcript variant X2, mRNA.

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CloneID	OHu98441
Clone ID Related Accession (Same CDS sequence)	XM_017014266.1 , XM_017014266.2
Accession Version	XM_017014266.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGAGT AAAATGGCCC GCTGGCCCAA AGAGCAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGG TCCCTGGTTT GCCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869755.1
CDS	399..5546
Translation	MDVHTRWKARSALRPGAPLLPPLLLLLLWAPPPSRAAQPADLLKVLDFHNLPDGITKTTGFCATRRSSKGPDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQIGLELGRSPVFLYEDHTGKPGPEDYPLFRGINLSDGKWHRIALSVHKKNVTLILDCKKKTTKFLDRSDHPMIDINGIIVFGTRILDEEVFEGDIQQLLFVSDHRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYTEGDGEGETYYYEYPYYEDPEDLGKEPTPSKKPVEAAKETTEVPEELTPTPTEAAPMPETSEGAGKEEDVGIGDYDYVPSEDYYTPSPYDDLTYGEGEENPDQPTDPGAGAEIPTSTADTSNSSNPAPPPGEGADDLEGEFTEETIRNLDENYYDPYYDPTSSPSEIGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPPGTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPVGPPGSGGLKGEPGDVGPQGPRGVQGPPGPAGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGPPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGLAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGNEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEKGESGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGEQGITGPSGPIGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHPGPPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGNGENYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGSKMARWPKEQPSTWYSQYKRGSLVCP

Target ORF information:

RefSeq Version	XM_017014266.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 1 (COL5A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014266.1

ORF Insert Sequence:

ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGAGT AAAATGGCCC GCTGGCCCAA AGAGCAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGG TCCCTGGTTT GCCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL5A1 ( XM_017014266.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869755.1 Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu98441
Clone ID Related Accession (Same CDS sequence)	XM_017014266.1 , XM_017014266.2
Accession Version	XM_017014266.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-1(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGAGT AAAATGGCCC GCTGGCCCAA AGAGCAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGG TCCCTGGTTT GCCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869755.1
CDS	400..5547
Translation	MDVHTRWKARSALRPGAPLLPPLLLLLLWAPPPSRAAQPADLLKVLDFHNLPDGITKTTGFCATRRSSKGPDVAYRVTKDAQLSAPTKQLYPASAFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQIGLELGRSPVFLYEDHTGKPGPEDYPLFRGINLSDGKWHRIALSVHKKNVTLILDCKKKTTKFLDRSDHPMIDINGIIVFGTRILDEEVFEGDIQQLLFVSDHRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYTEGDGEGETYYYEYPYYEDPEDLGKEPTPSKKPVEAAKETTEVPEELTPTPTEAAPMPETSEGAGKEEDVGIGDYDYVPSEDYYTPSPYDDLTYGEGEENPDQPTDPGAGAEIPTSTADTSNSSNPAPPPGEGADDLEGEFTEETIRNLDENYYDPYYDPTSSPSEIGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPPGTMGPTGQVGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPVGPPGSGGLKGEPGDVGPQGPRGVQGPPGPAGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGPPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPPGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGLAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGNEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGPRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPPGPKGERGEKGESGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGEQGITGPSGPIGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHPGPPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGNGENYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGSKMARWPKEQPSTWYSQYKRGSLVCP

Target ORF information:

RefSeq Version	XM_017014266.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type V alpha 1 chain (COL5A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014266.2

ORF Insert Sequence:

ATGGACGTCC ATACCCGCTG GAAAGCGCGC AGCGCGCTCC GCCCGGGCGC CCCGCTGCTG 
CCCCCGCTGC TGCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAGCA 
GATCTCCTGA AGGTTCTAGA TTTTCACAAC TTGCCTGATG GAATAACAAA GACAACAGGC 
TTTTGCGCCA CGCGGCGATC TTCCAAAGGC CCGGATGTCG CTTACAGAGT CACCAAAGAC 
GCGCAGCTCA GCGCACCCAC CAAGCAGCTG TACCCTGCGT CTGCATTTCC CGAGGACTTC 
TCCATCCTAA CAACTGTGAA AGCCAAGAAA GGCAGCCAGG CCTTCCTGGT CTCCATCTAC 
AACGAGCAGG GTATCCAGCA GATTGGGCTG GAGCTGGGCC GCTCTCCCGT CTTCCTCTAC 
GAGGACCACA CGGGGAAGCC TGGCCCGGAA GACTACCCCC TCTTCCGGGG CATCAACCTG 
TCAGATGGCA AGTGGCACAG AATTGCTCTC AGCGTCCACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAC CACCAAATTC CTCGACCGCA GCGACCACCC CATGATCGAC 
ATCAATGGCA TCATCGTGTT TGGCACCCGG ATCCTGGATG AGGAGGTGTT TGAGGGTGAC 
ATCCAGCAGC TGCTCTTTGT CTCGGACCAC CGGGCAGCTT ATGATTACTG TGAGCACTAC 
AGCCCTGACT GTGACACCGC AGTACCTGAC ACCCCACAGT CGCAGGACCC CAATCCAGAT 
GAATATTACA CGGAAGGAGA CGGCGAGGGT GAGACCTATT ACTACGAATA CCCCTACTAC 
GAAGACCCCG AAGACCTAGG GAAGGAGCCC ACCCCCAGCA AGAAGCCCGT GGAAGCTGCC 
AAAGAAACCA CAGAGGTCCC CGAGGAGCTG ACCCCGACCC CCACGGAAGC TGCTCCCATG 
CCTGAAACCA GTGAAGGGGC TGGGAAGGAA GAGGACGTCG GCATCGGGGA CTATGACTAC 
GTGCCCAGTG AGGACTACTA CACGCCCTCA CCGTATGATG ACCTCACCTA TGGCGAGGGG 
GAGGAGAACC CCGACCAGCC CACAGACCCA GGCGCTGGGG CCGAAATTCC CACCAGCACC 
GCCGACACCT CCAACTCCTC CAATCCAGCT CCGCCTCCAG GGGAAGGTGC GGATGACTTG 
GAGGGGGAGT TCACTGAGGA AACGATCCGG AACCTTGACG AGAACTACTA CGACCCCTAC 
TACGACCCCA CCAGCTCCCC GTCGGAGATC GGGCCGGGAA TGCCGGCGAA CCAGGATACC 
ATCTATGAAG GGATTGGAGG ACCTCGGGGC GAGAAAGGCC AAAAGGGAGA ACCAGCGATT 
ATCGAGCCGG GCATGCTCAT CGAGGGCCCG CCTGGCCCAG AAGGCCCCGC GGGTCTTCCC 
GGACCTCCAG GAACCATGGG TCCCACTGGC CAAGTCGGGG ACCCTGGAGA AAGGGGCCCC 
CCTGGACGCC CAGGCCTTCC TGGGGCCGAT GGCCTGCCCG GTCCTCCAGG AACCATGCTC 
ATGCTGCCCT TCCGGTTTGG AGGTGGCGGC GATGCGGGCT CCAAAGGCCC CATGGTCTCA 
GCCCAGGAGT CCCAGGCGCA AGCCATTCTC CAGCAGGCCA GGTTGGCACT GAGGGGACCA 
GCTGGCCCGA TGGGTCTCAC AGGGAGACCT GGCCCTGTGG GTCCCCCTGG GAGCGGAGGT 
TTGAAGGGCG AGCCGGGAGA CGTGGGGCCT CAGGGTCCTC GAGGTGTGCA AGGCCCGCCT 
GGTCCGGCCG GGAAGCCCGG AAGACGGGGT CGGGCTGGGA GTGATGGAGC CAGAGGAATG 
CCTGGACAAA CTGGCCCCAA GGGTGACCGG GGTTTCGACG GCCTGGCTGG GTTGCCAGGC 
GAGAAGGGCC ACAGGGGTGA CCCTGGTCCT TCCGGCCCAC CAGGACCTCC GGGAGACGAT 
GGAGAAAGGG GTGACGACGG AGAAGTTGGG CCCAGGGGGC TGCCTGGGGA GCCCGGGCCA 
CGTGGTCTGC TTGGGCCGAA GGGGCCCCCA GGTCCTCCCG GACCTCCCGG TGTCACGGGT 
ATGGACGGCC AGCCGGGGCC AAAAGGAAAT GTGGGTCCCC AGGGAGAGCC TGGCCCCCCA 
GGACAGCAGG GTAATCCAGG CGCCCAGGGT CTTCCAGGCC CCCAGGGTGC AATTGGTCCT 
CCAGGAGAAA AGGGTCCCTT GGGGAAACCA GGCCTTCCAG GAATGCCCGG TGCTGACGGA 
CCCCCGGGAC ACCCTGGCAA AGAAGGCCCT CCAGGAGAGA AAGGAGGTCA GGGTCCACCT 
GGCCCCCAGG GTCCGATTGG CTACCCAGGT CCTCGAGGAG TCAAGGGGGC CGATGGCATC 
CGTGGTCTGA AGGGCACAAA GGGCGAGAAG GGTGAAGACG GCTTTCCTGG GTTTAAAGGA 
GACATGGGCA TCAAGGGTGA TCGGGGGGAG ATCGGCCCAC CCGGTCCCAG GGGAGAAGAT 
GGCCCTGAAG GCCCAAAGGG TCGCGGAGGT CCCAATGGTG ACCCCGGTCC TCTGGGACCC 
CCTGGGGAGA AGGGAAAACT CGGAGTCCCA GGGTTACCAG GGTATCCAGG AAGACAAGGA 
CCAAAGGGCT CTATTGGATT CCCTGGATTT CCTGGCGCCA ATGGAGAGAA GGGCGGCAGG 
GGGACCCCTG GAAAGCCAGG ACCGCGGGGG CAGCGAGGCC CAACGGGTCC GAGGGGTGAA 
AGAGGCCCCC GGGGCATCAC TGGGAAGCCT GGCCCCAAGG GCAACTCCGG AGGTGACGGC 
CCAGCTGGCC CTCCTGGTGA ACGGGGACCC AATGGACCCC AAGGACCCAC AGGATTTCCT 
GGACCAAAGG GCCCCCCTGG CCCTCCAGGC AAGGATGGAC TCCCAGGACA CCCTGGACAG 
AGAGGCGAGA CTGGTTTCCA AGGCAAGACC GGCCCTCCAG GCCCCCCCGG CGTGGTCGGC 
CCTCAGGGTC CCACGGGAGA AACGGGCCCA ATGGGTGAGC GTGGCCACCC TGGGCCCCCT 
GGACCCCCCG GTGAACAGGG GCTTCCGGGC CTTGCTGGAA AAGAAGGGAC GAAGGGTGAC 
CCAGGCCCTG CAGGCCTCCC TGGGAAAGAT GGCCCTCCAG GATTACGTGG TTTCCCTGGG 
GACCGAGGGC TTCCTGGTCC AGTGGGAGCT CTTGGACTGA AAGGCAATGA AGGGCCCCCT 
GGCCCACCAG GCCCTGCGGG ATCTCCAGGG GAGAGAGGTC CAGCTGGAGC CGCTGGGCCC 
ATCGGAATTC CAGGGAGACC TGGGCCCCAG GGACCCCCAG GGCCGGCAGG AGAGAAAGGG 
GCTCCTGGCG AGAAAGGCCC ACAAGGCCCA GCTGGCCGAG ACGGTCTCCA GGGGCCTGTG 
GGGCTCCCGG GTCCAGCTGG CCCTGTGGGT CCCCCTGGAG AAGACGGAGA TAAGGGAGAG 
ATCGGGGAGC CGGGGCAGAA AGGAAGCAAG GGGGACAAAG GAGAACAGGG TCCTCCTGGG 
CCTACAGGTC CTCAAGGCCC CATCGGACAG CCAGGCCCCT CTGGAGCTGA CGGCGAGCCG 
GGGCCTCGGG GCCAGCAGGG CCTTTTCGGG CAGAAAGGTG ATGAAGGTCC CAGAGGCTTT 
CCTGGACCCC CTGGGCCAGT GGGGCTGCAG GGTTTGCCAG GACCTCCAGG CGAGAAGGGT 
GAGACAGGAG ACGTGGGCCA GATGGGCCCC CCGGGTCCCC CTGGCCCCCG AGGACCCTCC 
GGAGCTCCAG GTGCTGATGG CCCACAAGGT CCCCCAGGTG GAATAGGAAA CCCTGGTGCA 
GTGGGAGAGA AGGGCGAGCC TGGCGAAGCA GGTGAGCCTG GCCTTCCGGG AGAAGGCGGC 
CCCCCGGGAC CCAAAGGAGA AAGGGGAGAG AAGGGCGAGT CAGGCCCTTC AGGTGCTGCC 
GGACCCCCTG GACCCAAAGG CCCTCCCGGA GATGATGGTC CCAAAGGCAG CCCTGGCCCA 
GTGGGTTTTC CTGGAGATCC TGGCCCCCCC GGAGAGCCTG GCCCCGCGGG TCAAGATGGT 
CCCCCTGGTG ACAAAGGAGA TGATGGTGAA CCCGGGCAGA CGGGATCCCC CGGCCCTACT 
GGTGAACCAG GTCCATCGGG GCCTCCAGGA AAAAGGGGTC CCCCAGGCCC CGCAGGCCCC 
GAAGGCAGAC AGGGAGAGAA AGGGGCCAAG GGAGAAGCCG GCTTGGAAGG CCCTCCTGGG 
AAGACTGGCC CCATCGGCCC CCAGGGGGCC CCTGGGAAGC CCGGACCGGA TGGCCTTCGA 
GGGATCCCTG GCCCTGTGGG AGAACAAGGT CTCCCAGGAT CCCCAGGCCC GGACGGTCCC 
CCCGGCCCCA TGGGTCCCCC AGGACTTCCC GGCCTCAAAG GAGATTCTGG TCCCAAAGGT 
GAAAAGGGTC ATCCAGGCCT GATCGGGCTC ATCGGTCCTC CGGGTGAACA GGGTGAGAAG 
GGCGACCGTG GTCTCCCTGG CCCCCAGGGC TCCTCCGGTC CTAAGGGAGA ACAGGGTATC 
ACTGGTCCTT CTGGCCCGAT TGGGCCTCCT GGGCCCCCTG GCCTGCCGGG TCCGCCTGGT 
CCAAAAGGTG CTAAGGGCTC CTCGGGTCCA ACTGGCCCGA AGGGTGAGGC AGGCCACCCA 
GGACCCCCAG GCCCCCCGGG CCCCCCGGGA GAGGTCATCC AGCCCCTGCC AATCCAGGCA 
TCCAGGACGC GGCGGAACAT CGACGCCAGC CAGCTGCTGG ACGACGGGAA TGGCGAGAAC 
TACGTGGACT ACGCGGACGG CATGGAAGAG ATCTTCGGCT CTCTCAACTC TCTGAAGCTG 
GAGATTGAGC AGATGAAACG GCCCCTGGGC ACGCAGCAGA ACCCCGCCCG CACCTGCAAG 
GACCTGCAGC TCTGCCACCC CGACTTCCCA GATGGTGAAT ACTGGGTCGA TCCTAACCAA 
GGATGCTCCA GGGATTCCTT CAAGGTTTAC TGCAACTTCA CAGCCGGGGG GTCGACATGC 
GTCTTCCCTG ACAAGAAGTC CGAAGGGAGT AAAATGGCCC GCTGGCCCAA AGAGCAGCCT 
TCCACCTGGT ATAGTCAGTA CAAGCGGGGG TCCCTGGTTT GCCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of tissue-specific and developmentally regulated alternative splicing of exon 64 in the COL5A1 gene.
Connective tissue research53(3)267-76(2012)
Mitchell AL,Judis LM,Schwarze U,Vaynshtok PM,Drumm ML,Byers PH

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COL5A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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