COL4A4 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011510560.2	XP_011508862.1	collagen alpha-4(IV) chain isoform X7
XM_011510565.2	XP_011508867.1	collagen alpha-4(IV) chain isoform X11
NM_000092.5	NP_000083.3	collagen alpha-4(IV) chain precursor
XM_017003298.1	XP_016858787.1	collagen alpha-4(IV) chain isoform X8
XM_017003299.1	XP_016858788.1	collagen alpha-4(IV) chain isoform X9
XM_005246282.3	XP_005246339.1	collagen alpha-4(IV) chain isoform X10
XM_011510566.2	XP_011508868.1	collagen alpha-4(IV) chain isoform X12
XM_011510567.2	XP_011508869.1	collagen alpha-4(IV) chain isoform X12
XM_011510572.3	XP_011508874.1	collagen alpha-4(IV) chain isoform X15
NM_000092.4	NP_000083.3	collagen alpha-4(IV) chain precursor
XM_011510568.2	XP_011508870.1	collagen alpha-4(IV) chain isoform X13
XM_011510570.2	XP_011508872.1	collagen alpha-4(IV) chain isoform X14
XM_011510569.2	XP_011508871.1	collagen alpha-4(IV) chain isoform X14
XM_005246281.2	XP_005246338.1	collagen alpha-4(IV) chain isoform X1
XM_011510557.2	XP_011508859.1	collagen alpha-4(IV) chain isoform X2
XM_017003297.1	XP_016858786.1	collagen alpha-4(IV) chain isoform X5
XM_006712246.3	XP_006712309.1	collagen alpha-4(IV) chain isoform X6
XM_011510562.2	XP_011508864.1	collagen alpha-4(IV) chain isoform X8
XM_017003300.1	XP_016858789.1	collagen alpha-4(IV) chain isoform X14
XM_011510572.2	XP_011508874.1	collagen alpha-4(IV) chain isoform X15
XM_011510559.2	XP_011508861.1	collagen alpha-4(IV) chain isoform X7
XM_011510561.2	XP_011508863.1	collagen alpha-4(IV) chain isoform X8
XM_005246281.3	XP_005246338.1	collagen alpha-4(IV) chain isoform X1
XM_017003296.1	XP_016858785.1	collagen alpha-4(IV) chain isoform X3
XM_011510558.2	XP_011508860.1	collagen alpha-4(IV) chain isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19492	NM_000092.5 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu19492	XM_005246281.3 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu35833	XM_006712246.3 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu35834	XM_011510559.2 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu35834	XM_011510560.2 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu35836	XM_017003298.1 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu35836	XM_011510561.2 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu35836	XM_011510562.2 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu35837	XM_005246282.3 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu35839	XM_011510567.2 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu35839	XM_011510566.2 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu56971	XM_011510557.2 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu56972	XM_011510558.2 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu56975	XM_011510565.2 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu56976	XM_011510568.2 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu56977	XM_017003300.1 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu56977	XM_011510570.2 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu56977	XM_011510569.2 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu56978	XM_011510572.3 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu88997	XM_017003296.1 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu88998	XM_017003297.1 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu88999	XM_017003299.1 Latest version!	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
View Old Accession Versions >>
OHu19492	NM_000092.4	Homo sapiens collagen type IV alpha 4 chain (COL4A4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu56978	XM_011510572.2	Homo sapiens collagen type IV alpha 4 (COL4A4), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu19492	XM_005246281.2	Homo sapiens collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

COL4A4 ( NM_000092.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000083.3 Homo sapiens collagen type IV alpha 4 chain (COL4A4), mRNA.

Price & Availability↑

CloneID	OHu19492
Clone ID Related Accession (Same CDS sequence)	NM_000092.5 , NM_000092.4 , XM_005246281.2 , XM_005246281.3
Accession Version	NM_000092.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC079235.7, X81053.1, BX094848.1 and AC073149.5. On Aug 31, 2019 this sequence version replaced NM_000092.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X81053.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000083.3
CDS	301..5373
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	NM_000092.5
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000092.5

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( NM_000092.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000083.3 Homo sapiens collagen type IV alpha 4 chain (COL4A4), mRNA.

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CloneID	OHu19492
Clone ID Related Accession (Same CDS sequence)	NM_000092.5 , NM_000092.4 , XM_005246281.2 , XM_005246281.3
Accession Version	NM_000092.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-06
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC079235.7, X81053.1, BX094848.1 and AC073149.5. This sequence is a reference standard in the RefSeqGene project. On Oct 17, 2006 this sequence version replaced NM_000092.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X81053.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000083.3
CDS	655..5727
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	NM_000092.4
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000092.4

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_005246281.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246338.1 Homo sapiens collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA.

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CloneID	OHu19492
Clone ID Related Accession (Same CDS sequence)	NM_000092.5 , NM_000092.4 , XM_005246281.2 , XM_005246281.3
Accession Version	XM_005246281.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	collagen alpha-4(IV) chain isoform X1
Comment

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005246338.1

Translation

MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_005246281.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246281.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_005246281.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246338.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu19492
Clone ID Related Accession (Same CDS sequence)	NM_000092.5 , NM_000092.4 , XM_005246281.2 , XM_005246281.3
Accession Version	XM_005246281.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005246281.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246338.1
CDS	189..5261
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_005246281.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246281.3

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TCGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_006712246.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712309.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X6, mRNA.

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CloneID	OHu35833
Clone ID Related Accession (Same CDS sequence)	XM_006712246.3
Accession Version	XM_006712246.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712246.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGTCTGGC AGGGTCTTGC CTTCCCGTAT TTAGCACGCT GCCCTTTGCC 
TACTGCAACA TCCACCAGGT GTGCCACTAT GCCCAGAGAA ACGACAGATC CTACTGGCTG 
GCCAGCGCTG CGCCCCTCCC CATGATGCCA CTCTCTGAAG AGGCGATCCG CCCCTATGTC 
AGCCGCTGTG CGGTATGCGA GGCCCCGGCC CAGGCGGTGG CGGTGCACAG CCAGGACCAG 
TCCATCCCCC CATGTCCGCA GACCTGGAGG AGCCTCTGGA TCGGGTATTC ATTCCTGATG 
CACACAGGAG CTGGGGACCA AGGAGGAGGG CAGGCCCTTA TGTCACCTGG CAGCTGCCTG 
GAAGATTTCA GAGCAGCACC ATTCCTTGAA TGCCAGGGCC GGCAGGGAAC TTGCCACTTT 
TTCGCAAATA AGTATAGCTT CTGGCTCACA ACGGTGAAAG CAGACTTGCA GTTTTCCTCT 
GCTCCAGCAC CAGACACCTT AAAAGAAAGC CAGGCCCAAC GCCAGAAAAT CAGCCGGTGC 
CAGGTCTGCG TGAAGTATAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712309.1
CDS	327..5210
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_006712246.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712246.3

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGTCTGGC AGGGTCTTGC CTTCCCGTAT TTAGCACGCT GCCCTTTGCC 
TACTGCAACA TCCACCAGGT GTGCCACTAT GCCCAGAGAA ACGACAGATC CTACTGGCTG 
GCCAGCGCTG CGCCCCTCCC CATGATGCCA CTCTCTGAAG AGGCGATCCG CCCCTATGTC 
AGCCGCTGTG CGGTATGCGA GGCCCCGGCC CAGGCGGTGG CGGTGCACAG CCAGGACCAG 
TCCATCCCCC CATGTCCGCA GACCTGGAGG AGCCTCTGGA TCGGGTATTC ATTCCTGATG 
CACACAGGAG CTGGGGACCA AGGAGGAGGG CAGGCCCTTA TGTCACCTGG CAGCTGCCTG 
GAAGATTTCA GAGCAGCACC ATTCCTTGAA TGCCAGGGCC GGCAGGGAAC TTGCCACTTT 
TTCGCAAATA AGTATAGCTT CTGGCTCACA ACGGTGAAAG CAGACTTGCA GTTTTCCTCT 
GCTCCAGCAC CAGACACCTT AAAAGAAAGC CAGGCCCAAC GCCAGAAAAT CAGCCGGTGC 
CAGGTCTGCG TGAAGTATAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510560.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508862.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X11, mRNA.

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CloneID	OHu35834
Clone ID Related Accession (Same CDS sequence)	XM_011510560.2 , XM_011510559.2
Accession Version	XM_011510560.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGG TGGAACTAGA GGAAGACAAA CTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508862.1
CDS	327..5165
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMVELEEDKLL

Target ORF information:

RefSeq Version	XM_011510560.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510560.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGG TGGAACTAGA GGAAGACAAA CTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510559.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508861.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X9, mRNA.

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CloneID	OHu35834
Clone ID Related Accession (Same CDS sequence)	XM_011510560.2 , XM_011510559.2
Accession Version	XM_011510559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510559.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGG TGGAACTAGA GGAAGACAAA CTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508861.1
CDS	327..5165
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMVELEEDKLL

Target ORF information:

RefSeq Version	XM_011510559.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510559.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGG TGGAACTAGA GGAAGACAAA CTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_017003298.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858787.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X12, mRNA.

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CloneID	OHu35836
Clone ID Related Accession (Same CDS sequence)	XM_017003298.1 , XM_011510562.2 , XM_011510561.2
Accession Version	XM_017003298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858787.1
CDS	327..5153
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMLMGVF

Target ORF information:

RefSeq Version	XM_017003298.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003298.1

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510562.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508864.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X13, mRNA.

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CloneID	OHu35836
Clone ID Related Accession (Same CDS sequence)	XM_017003298.1 , XM_011510562.2 , XM_011510561.2
Accession Version	XM_011510562.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508864.1
CDS	327..5153
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMLMGVF

Target ORF information:

RefSeq Version	XM_011510562.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510562.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510561.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508863.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu35836
Clone ID Related Accession (Same CDS sequence)	XM_017003298.1 , XM_011510562.2 , XM_011510561.2
Accession Version	XM_011510561.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508863.1
CDS	327..5153
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMLMGVF

Target ORF information:

RefSeq Version	XM_011510561.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510561.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_005246282.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246339.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X16, mRNA.

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CloneID	OHu35837
Clone ID Related Accession (Same CDS sequence)	XM_005246282.3
Accession Version	XM_005246282.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005246282.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGAAATG AAGGACTCTG TGCCTGTGAG CCTGGACCCA TGGGCCCCCC TGGCCCTCCA 
GGACTTCCTG GGAGGCAGGG GAGTAAGGGA GACTTGGGGC TCCCTGGCTG GCTTGGAACA 
AAAGGTGACC CAGGACCTCC TGGTGCTGAA GGACCTCCAG GGCTACCAGG AAAGCATGGT 
GCCTCTGGAC CACCTGGCAA CAAAGGGGCG AAGGGTGACA TGGTTGTATC AAGAGTTAAA 
GGGCACAAAG GAGAAAGAGG TCCTGATGGG CCCCCAGGAT TTCCAGGGCA GCCAGGATCA 
CATGGTCGGG ATGGACATGC TGGAGAAAAA GGGGATCCAG GACCTCCAGG GGATCATGAA 
GATGCGACCC CAGGTGGTAA AGGATTTCCT GGACCTCTGG GCCCCCCAGG CAAAGCAGGA 
CCTGTGGGGC CCCCAGGACT GGGATTTCCT GGTCCACCAG GAGAGCGAGG CCACCCAGGA 
GTTCCAGGCC ACCCAGGTGT GAGGGGCCCT GATGGCTTGA AGGGTCAGAA AGGTGACACA 
ATTTCTTGCA ACGTAACCTA CCCTGGGAGG CATGGCCCTC CAGGTTTTGA TGGACCTCCA 
GGTCCGAAGG GATTTCCAGG TCCCCAAGGT GCCCCTGGGC TGAGTGGTTC AGATGGGCAT 
AAAGGCAGAC CTGGCACACC AGGAACAGCG GAAATACCAG GTCCACCTGG TTTTCGTGGT 
GACATGGGAG ATCCGGGTTT TGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGC 
CCTCCCGGCT CACCAGGAGT GAATGGTCAG AAAGGAATCC CGGGAGACCC TGCATTTGGT 
CACCTGGGAC CCCCGGGAAA GAGGGGTCTT TCAGGAGTGC CAGGGATAAA AGGACCCAGA 
GGTGATCCGG GATGTCCAGG GGCTGAAGGG CCAGCTGGCA TTCCTGGATT CCTAGGTCTC 
AAAGGTCCCA AAGGCAGAGA GGGACATGCT GGGTTTCCAG GTGTCCCAGG TCCACCTGGC 
CATTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGACTCCC TGGGTATCCA 
GGTAGCCCAG GTGCTCCAGG TGGGAAAGGA CAGCCGGGAG ATGTGGGGCC TCCCGGGCCA 
GCTGGAATGA AAGGCCTCCC CGGACTCCCA GGACGGCCTG GGGCACATGG TCCCCCAGGC 
CTCCCAGGAA TCCCAGGTCC CTTTGGAGAT GATGGGCTAC CTGGTCCTCC AGGTCCAAAG 
GGACCCCGGG GGCTGCCTGG TTTCCCAGGT TTTCCCGGAG AAAGAGGAAA GCCTGGTGCA 
GAGGGATGTC CTGGCGCAAA GGGAGAACCT GGAGAGAAGG GCATGTCTGG CCTTCCTGGA 
GACCGGGGAC TGAGAGGGGC CAAAGGAGCC ATAGGACCTC CCGGAGATGA AGGAGAAATG 
GCTATCATTT CACAAAAGGG AACACCTGGG GAACCTGGAC CTCCTGGAGA TGATGGATTC 
CCAGGAGAAA GAGGTGATAA AGGAACTCCC GGGATGCAAG GGAGAAGAGG AGAGCCGGGA 
AGATACGGAC CACCTGGATT TCACAGAGGG GAACCTGGTG AGAAAGGTCA GCCAGGGCCT 
CCTGGACCCC CAGGCCCTCC AGGCTCAACT GGTCTAAGAG GGTTCATTGG TTTTCCAGGA 
CTTCCAGGTG ACCAGGGTGA GCCAGGTTCT CCAGGTCCCC CTGGATTTTC AGGAATTGAT 
GGAGCAAGAG GACCTAAAGG AAACAAAGGT GACCCTGCCA GTCACTTTGG TCCACCTGGT 
CCAAAGGGTG AGCCAGGTAG CCCTGGATGT CCAGGGCATT TTGGAGCATC CGGAGAGCAG 
GGCTTGCCTG GTATTCAAGG GCCCAGAGGA TCACCTGGAA GGCCAGGGCC ACCTGGCTCC 
TCTGGACCAC CAGGGTGCCC AGGTGATCAC GGGATGCCTG GGCTGAGGGG ACAGCCAGGA 
GAAATGGGAG ACCCTGGGCC AAGAGGCCTC CAGGGGGATC CAGGGATACC AGGTCCTCCG 
GGAATAAAAG GTCCCTCCGG ATCACCTGGC CTGAACGGCT TGCATGGATT GAAAGGTCAG 
AAAGGAACTA AAGGTGCTTC AGGTTTGCAT GATGTGGGGC CACCTGGTCC AGTGGGAATA 
CCTGGGCTAA AAGGGGAGAG AGGAGACCCT GGGAGCCCAG GAATCTCTCC TCCAGGTCCT 
CGTGGAAAGA AAGGTCCCCC AGGACCCCCA GGGAGTTCAG GACCACCTGG TCCTGCAGGT 
GCCACAGGAA GAGCTCCTAA GGACATTCCT GACCCGGGTC CACCTGGAGA TCAGGGACCT 
CCTGGTCCTG ATGGCCCAAG AGGAGCACCT GGGCCTCCAG GCCTCCCTGG GAGTGTTGAC 
CTTCTGAGAG GGGAGCCAGG TGACTGTGGT CTACCAGGGC CACCAGGTCC CCCTGGCCCA 
CCAGGCCCTC CAGGATACAA AGGCTTTCCA GGATGTGATG GAAAAGATGG CCAGAAAGGA 
CCAGTGGGAT TCCCGGGACC GCAGGGACCA CATGGATTTC CTGGGCCACC TGGAGAGAAG 
GGTTTACCTG GACCTCCAGG GAGAAAAGGG CCCACTGGTC TTCCGGGTCC CAGAGGTGAA 
CCGGGGCCAC CTGCAGATGT GGATGACTGT CCCCGAATCC CAGGCCTTCC TGGGGCGCCA 
GGCATGAGAG GACCAGAAGG AGCCATGGGG CTCCCTGGAA TGAGAGGCCC CTCAGGACCA 
GGGTGCAAAG GAGAGCCTGG GCTGGATGGC AGGAGGGGTG TGGATGGCGT CCCTGGGTCT 
CCTGGGCCTC CCGGACGTAA AGGTGACACA GGAGAAGACG GCTACCCTGG AGGACCAGGG 
CCTCCTGGTC CCATTGGGGA TCCTGGGCCC AAAGGGTTTG GCCCTGGATA CCTCGGTGGC 
TTCCTCCTGG TTCTCCACAG TCAGACGGAC CAGGAGCCCA CCTGCCCCCT GGGCATGCCC 
AGGCTCTGGA CTGGGTATAG TCTGTTATAC CTGGAAGGGC AAGAGAAAGC TCACAATCAA 
GACCTTGGTC TGGCAGGGTC TTGCCTTCCC GTATTTAGCA CGCTGCCCTT TGCCTACTGC 
AACATCCACC AGGTGTGCCA CTATGCCCAG AGAAACGACA GATCCTACTG GCTGGCCAGC 
GCTGCGCCCC TCCCCATGAT GCCACTCTCT GAAGAGGCGA TCCGCCCCTA TGTCAGCCGC 
TGTGCGGTAT GCGAGGCCCC GGCCCAGGCG GTGGCGGTGC ACAGCCAGGA CCAGTCCATC 
CCCCCATGTC CGCAGACCTG GAGGAGCCTC TGGATCGGGT ATTCATTCCT GATGCACACA 
GGAGCTGGGG ACCAAGGAGG AGGGCAGGCC CTTATGTCAC CTGGCAGCTG CCTGGAAGAT 
TTCAGAGCAG CACCATTCCT TGAATGCCAG GGCCGGCAGG GAACTTGCCA CTTTTTCGCA 
AATAAGTATA GCTTCTGGCT CACAACGGTG AAAGCAGACT TGCAGTTTTC CTCTGCTCCA 
GCACCAGACA CCTTAAAAGA AAGCCAGGCC CAACGCCAGA AAATCAGCCG GTGCCAGGTC 
TGCGTGAAGT ATAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246339.1
CDS	327..4844
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_005246282.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246282.3

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGAAATG AAGGACTCTG TGCCTGTGAG CCTGGACCCA TGGGCCCCCC TGGCCCTCCA 
GGACTTCCTG GGAGGCAGGG GAGTAAGGGA GACTTGGGGC TCCCTGGCTG GCTTGGAACA 
AAAGGTGACC CAGGACCTCC TGGTGCTGAA GGACCTCCAG GGCTACCAGG AAAGCATGGT 
GCCTCTGGAC CACCTGGCAA CAAAGGGGCG AAGGGTGACA TGGTTGTATC AAGAGTTAAA 
GGGCACAAAG GAGAAAGAGG TCCTGATGGG CCCCCAGGAT TTCCAGGGCA GCCAGGATCA 
CATGGTCGGG ATGGACATGC TGGAGAAAAA GGGGATCCAG GACCTCCAGG GGATCATGAA 
GATGCGACCC CAGGTGGTAA AGGATTTCCT GGACCTCTGG GCCCCCCAGG CAAAGCAGGA 
CCTGTGGGGC CCCCAGGACT GGGATTTCCT GGTCCACCAG GAGAGCGAGG CCACCCAGGA 
GTTCCAGGCC ACCCAGGTGT GAGGGGCCCT GATGGCTTGA AGGGTCAGAA AGGTGACACA 
ATTTCTTGCA ACGTAACCTA CCCTGGGAGG CATGGCCCTC CAGGTTTTGA TGGACCTCCA 
GGTCCGAAGG GATTTCCAGG TCCCCAAGGT GCCCCTGGGC TGAGTGGTTC AGATGGGCAT 
AAAGGCAGAC CTGGCACACC AGGAACAGCG GAAATACCAG GTCCACCTGG TTTTCGTGGT 
GACATGGGAG ATCCGGGTTT TGGAGGTGAA AAGGGGTCCT CCCCTGTTGG GCCCCCAGGC 
CCTCCCGGCT CACCAGGAGT GAATGGTCAG AAAGGAATCC CGGGAGACCC TGCATTTGGT 
CACCTGGGAC CCCCGGGAAA GAGGGGTCTT TCAGGAGTGC CAGGGATAAA AGGACCCAGA 
GGTGATCCGG GATGTCCAGG GGCTGAAGGG CCAGCTGGCA TTCCTGGATT CCTAGGTCTC 
AAAGGTCCCA AAGGCAGAGA GGGACATGCT GGGTTTCCAG GTGTCCCAGG TCCACCTGGC 
CATTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGACTCCC TGGGTATCCA 
GGTAGCCCAG GTGCTCCAGG TGGGAAAGGA CAGCCGGGAG ATGTGGGGCC TCCCGGGCCA 
GCTGGAATGA AAGGCCTCCC CGGACTCCCA GGACGGCCTG GGGCACATGG TCCCCCAGGC 
CTCCCAGGAA TCCCAGGTCC CTTTGGAGAT GATGGGCTAC CTGGTCCTCC AGGTCCAAAG 
GGACCCCGGG GGCTGCCTGG TTTCCCAGGT TTTCCCGGAG AAAGAGGAAA GCCTGGTGCA 
GAGGGATGTC CTGGCGCAAA GGGAGAACCT GGAGAGAAGG GCATGTCTGG CCTTCCTGGA 
GACCGGGGAC TGAGAGGGGC CAAAGGAGCC ATAGGACCTC CCGGAGATGA AGGAGAAATG 
GCTATCATTT CACAAAAGGG AACACCTGGG GAACCTGGAC CTCCTGGAGA TGATGGATTC 
CCAGGAGAAA GAGGTGATAA AGGAACTCCC GGGATGCAAG GGAGAAGAGG AGAGCCGGGA 
AGATACGGAC CACCTGGATT TCACAGAGGG GAACCTGGTG AGAAAGGTCA GCCAGGGCCT 
CCTGGACCCC CAGGCCCTCC AGGCTCAACT GGTCTAAGAG GGTTCATTGG TTTTCCAGGA 
CTTCCAGGTG ACCAGGGTGA GCCAGGTTCT CCAGGTCCCC CTGGATTTTC AGGAATTGAT 
GGAGCAAGAG GACCTAAAGG AAACAAAGGT GACCCTGCCA GTCACTTTGG TCCACCTGGT 
CCAAAGGGTG AGCCAGGTAG CCCTGGATGT CCAGGGCATT TTGGAGCATC CGGAGAGCAG 
GGCTTGCCTG GTATTCAAGG GCCCAGAGGA TCACCTGGAA GGCCAGGGCC ACCTGGCTCC 
TCTGGACCAC CAGGGTGCCC AGGTGATCAC GGGATGCCTG GGCTGAGGGG ACAGCCAGGA 
GAAATGGGAG ACCCTGGGCC AAGAGGCCTC CAGGGGGATC CAGGGATACC AGGTCCTCCG 
GGAATAAAAG GTCCCTCCGG ATCACCTGGC CTGAACGGCT TGCATGGATT GAAAGGTCAG 
AAAGGAACTA AAGGTGCTTC AGGTTTGCAT GATGTGGGGC CACCTGGTCC AGTGGGAATA 
CCTGGGCTAA AAGGGGAGAG AGGAGACCCT GGGAGCCCAG GAATCTCTCC TCCAGGTCCT 
CGTGGAAAGA AAGGTCCCCC AGGACCCCCA GGGAGTTCAG GACCACCTGG TCCTGCAGGT 
GCCACAGGAA GAGCTCCTAA GGACATTCCT GACCCGGGTC CACCTGGAGA TCAGGGACCT 
CCTGGTCCTG ATGGCCCAAG AGGAGCACCT GGGCCTCCAG GCCTCCCTGG GAGTGTTGAC 
CTTCTGAGAG GGGAGCCAGG TGACTGTGGT CTACCAGGGC CACCAGGTCC CCCTGGCCCA 
CCAGGCCCTC CAGGATACAA AGGCTTTCCA GGATGTGATG GAAAAGATGG CCAGAAAGGA 
CCAGTGGGAT TCCCGGGACC GCAGGGACCA CATGGATTTC CTGGGCCACC TGGAGAGAAG 
GGTTTACCTG GACCTCCAGG GAGAAAAGGG CCCACTGGTC TTCCGGGTCC CAGAGGTGAA 
CCGGGGCCAC CTGCAGATGT GGATGACTGT CCCCGAATCC CAGGCCTTCC TGGGGCGCCA 
GGCATGAGAG GACCAGAAGG AGCCATGGGG CTCCCTGGAA TGAGAGGCCC CTCAGGACCA 
GGGTGCAAAG GAGAGCCTGG GCTGGATGGC AGGAGGGGTG TGGATGGCGT CCCTGGGTCT 
CCTGGGCCTC CCGGACGTAA AGGTGACACA GGAGAAGACG GCTACCCTGG AGGACCAGGG 
CCTCCTGGTC CCATTGGGGA TCCTGGGCCC AAAGGGTTTG GCCCTGGATA CCTCGGTGGC 
TTCCTCCTGG TTCTCCACAG TCAGACGGAC CAGGAGCCCA CCTGCCCCCT GGGCATGCCC 
AGGCTCTGGA CTGGGTATAG TCTGTTATAC CTGGAAGGGC AAGAGAAAGC TCACAATCAA 
GACCTTGGTC TGGCAGGGTC TTGCCTTCCC GTATTTAGCA CGCTGCCCTT TGCCTACTGC 
AACATCCACC AGGTGTGCCA CTATGCCCAG AGAAACGACA GATCCTACTG GCTGGCCAGC 
GCTGCGCCCC TCCCCATGAT GCCACTCTCT GAAGAGGCGA TCCGCCCCTA TGTCAGCCGC 
TGTGCGGTAT GCGAGGCCCC GGCCCAGGCG GTGGCGGTGC ACAGCCAGGA CCAGTCCATC 
CCCCCATGTC CGCAGACCTG GAGGAGCCTC TGGATCGGGT ATTCATTCCT GATGCACACA 
GGAGCTGGGG ACCAAGGAGG AGGGCAGGCC CTTATGTCAC CTGGCAGCTG CCTGGAAGAT 
TTCAGAGCAG CACCATTCCT TGAATGCCAG GGCCGGCAGG GAACTTGCCA CTTTTTCGCA 
AATAAGTATA GCTTCTGGCT CACAACGGTG AAAGCAGACT TGCAGTTTTC CTCTGCTCCA 
GCACCAGACA CCTTAAAAGA AAGCCAGGCC CAACGCCAGA AAATCAGCCG GTGCCAGGTC 
TGCGTGAAGT ATAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510566.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508868.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X21, mRNA.

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CloneID	OHu35839
Clone ID Related Accession (Same CDS sequence)	XM_011510566.2 , XM_011510567.2
Accession Version	XM_011510566.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGTGG AACTAGAGGA AGACAAACTG CTCTGAGAAA AACCTGCAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508868.1
CDS	327..4589
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGGTRGRQTALRKTCN

Target ORF information:

RefSeq Version	XM_011510566.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510566.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGTGG AACTAGAGGA AGACAAACTG CTCTGAGAAA AACCTGCAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510567.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508869.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OHu35839
Clone ID Related Accession (Same CDS sequence)	XM_011510566.2 , XM_011510567.2
Accession Version	XM_011510567.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510567.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGTGG AACTAGAGGA AGACAAACTG CTCTGAGAAA AACCTGCAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508869.1
CDS	327..4589
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGGTRGRQTALRKTCN

Target ORF information:

RefSeq Version	XM_011510567.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510567.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGTGG AACTAGAGGA AGACAAACTG CTCTGAGAAA AACCTGCAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510557.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508859.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu56971
Clone ID Related Accession (Same CDS sequence)	XM_011510557.2
Accession Version	XM_011510557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGACTTCCA 
GGTGACCAGG GTGAGCCAGG TTCTCCAGGT CCCCCTGGAT TTTCAGGAAT TGATGGAGCA 
AGAGGACCTA AAGGAAACAA AGGTGACCCT GCCAGTCACT TTGGTCCACC TGGTCCAAAG 
GGTGAGCCAG GTAGCCCTGG ATGTCCAGGG CATTTTGGAG CATCCGGAGA GCAGGGCTTG 
CCTGGTATTC AAGGGCCCAG AGGATCACCT GGAAGGCCAG GGCCACCTGG CTCCTCTGGA 
CCACCAGGGT GCCCAGGTGA TCACGGGATG CCTGGGCTGA GGGGACAGCC AGGAGAAATG 
GGAGACCCTG GGCCAAGAGG CCTCCAGGGG GATCCAGGGA TACCAGGTCC TCCGGGAATA 
AAAGGTCCCT CCGGATCACC TGGCCTGAAC GGCTTGCATG GATTGAAAGG TCAGAAAGGA 
ACTAAAGGTG CTTCAGGTTT GCATGATGTG GGGCCACCTG GTCCAGTGGG AATACCTGGG 
CTAAAAGGGG AGAGAGGAGA CCCTGGGAGC CCAGGAATCT CTCCTCCAGG TCCTCGTGGA 
AAGAAAGGTC CCCCAGGACC CCCAGGGAGT TCAGGACCAC CTGGTCCTGC AGGTGCCACA 
GGAAGAGCTC CTAAGGACAT TCCTGACCCG GGTCCACCTG GAGATCAGGG ACCTCCTGGT 
CCTGATGGCC CAAGAGGAGC ACCTGGGCCT CCAGGCCTCC CTGGGAGTGT TGACCTTCTG 
AGAGGGGAGC CAGGTGACTG TGGTCTACCA GGGCCACCAG GTCCCCCTGG CCCACCAGGC 
CCTCCAGGAT ACAAAGGCTT TCCAGGATGT GATGGAAAAG ATGGCCAGAA AGGACCAGTG 
GGATTCCCGG GACCGCAGGG ACCACATGGA TTTCCTGGGC CACCTGGAGA GAAGGGTTTA 
CCTGGACCTC CAGGGAGAAA AGGGCCCACT GGTCTTCCGG GTCCCAGAGG TGAACCGGGG 
CCACCTGCAG ATGTGGATGA CTGTCCCCGA ATCCCAGGCC TTCCTGGGGC GCCAGGCATG 
AGAGGACCAG AAGGAGCCAT GGGGCTCCCT GGAATGAGAG GCCCCTCAGG ACCAGGGTGC 
AAAGGAGAGC CTGGGCTGGA TGGCAGGAGG GGTGTGGATG GCGTCCCTGG GTCTCCTGGG 
CCTCCCGGAC GTAAAGGTGA CACAGGAGAA GACGGCTACC CTGGAGGACC AGGGCCTCCT 
GGTCCCATTG GGGATCCTGG GCCCAAAGGG TTTGGCCCTG GATACCTCGG TGGCTTCCTC 
CTGGTTCTCC ACAGTCAGAC GGACCAGGAG CCCACCTGCC CCCTGGGCAT GCCCAGGCTC 
TGGACTGGGT ATAGTCTGTT ATACCTGGAA GGGCAAGAGA AAGCTCACAA TCAAGACCTT 
GGTCTGGCAG GGTCTTGCCT TCCCGTATTT AGCACGCTGC CCTTTGCCTA CTGCAACATC 
CACCAGGTGT GCCACTATGC CCAGAGAAAC GACAGATCCT ACTGGCTGGC CAGCGCTGCG 
CCCCTCCCCA TGATGCCACT CTCTGAAGAG GCGATCCGCC CCTATGTCAG CCGCTGTGCG 
GTATGCGAGG CCCCGGCCCA GGCGGTGGCG GTGCACAGCC AGGACCAGTC CATCCCCCCA 
TGTCCGCAGA CCTGGAGGAG CCTCTGGATC GGGTATTCAT TCCTGATGCA CACAGGAGCT 
GGGGACCAAG GAGGAGGGCA GGCCCTTATG TCACCTGGCA GCTGCCTGGA AGATTTCAGA 
GCAGCACCAT TCCTTGAATG CCAGGGCCGG CAGGGAACTT GCCACTTTTT CGCAAATAAG 
TATAGCTTCT GGCTCACAAC GGTGAAAGCA GACTTGCAGT TTTCCTCTGC TCCAGCACCA 
GACACCTTAA AAGAAAGCCA GGCCCAACGC CAGAAAATCA GCCGGTGCCA GGTCTGCGTG 
AAGTATAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508859.1
CDS	327..5318
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_011510557.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510557.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGACTTCCA 
GGTGACCAGG GTGAGCCAGG TTCTCCAGGT CCCCCTGGAT TTTCAGGAAT TGATGGAGCA 
AGAGGACCTA AAGGAAACAA AGGTGACCCT GCCAGTCACT TTGGTCCACC TGGTCCAAAG 
GGTGAGCCAG GTAGCCCTGG ATGTCCAGGG CATTTTGGAG CATCCGGAGA GCAGGGCTTG 
CCTGGTATTC AAGGGCCCAG AGGATCACCT GGAAGGCCAG GGCCACCTGG CTCCTCTGGA 
CCACCAGGGT GCCCAGGTGA TCACGGGATG CCTGGGCTGA GGGGACAGCC AGGAGAAATG 
GGAGACCCTG GGCCAAGAGG CCTCCAGGGG GATCCAGGGA TACCAGGTCC TCCGGGAATA 
AAAGGTCCCT CCGGATCACC TGGCCTGAAC GGCTTGCATG GATTGAAAGG TCAGAAAGGA 
ACTAAAGGTG CTTCAGGTTT GCATGATGTG GGGCCACCTG GTCCAGTGGG AATACCTGGG 
CTAAAAGGGG AGAGAGGAGA CCCTGGGAGC CCAGGAATCT CTCCTCCAGG TCCTCGTGGA 
AAGAAAGGTC CCCCAGGACC CCCAGGGAGT TCAGGACCAC CTGGTCCTGC AGGTGCCACA 
GGAAGAGCTC CTAAGGACAT TCCTGACCCG GGTCCACCTG GAGATCAGGG ACCTCCTGGT 
CCTGATGGCC CAAGAGGAGC ACCTGGGCCT CCAGGCCTCC CTGGGAGTGT TGACCTTCTG 
AGAGGGGAGC CAGGTGACTG TGGTCTACCA GGGCCACCAG GTCCCCCTGG CCCACCAGGC 
CCTCCAGGAT ACAAAGGCTT TCCAGGATGT GATGGAAAAG ATGGCCAGAA AGGACCAGTG 
GGATTCCCGG GACCGCAGGG ACCACATGGA TTTCCTGGGC CACCTGGAGA GAAGGGTTTA 
CCTGGACCTC CAGGGAGAAA AGGGCCCACT GGTCTTCCGG GTCCCAGAGG TGAACCGGGG 
CCACCTGCAG ATGTGGATGA CTGTCCCCGA ATCCCAGGCC TTCCTGGGGC GCCAGGCATG 
AGAGGACCAG AAGGAGCCAT GGGGCTCCCT GGAATGAGAG GCCCCTCAGG ACCAGGGTGC 
AAAGGAGAGC CTGGGCTGGA TGGCAGGAGG GGTGTGGATG GCGTCCCTGG GTCTCCTGGG 
CCTCCCGGAC GTAAAGGTGA CACAGGAGAA GACGGCTACC CTGGAGGACC AGGGCCTCCT 
GGTCCCATTG GGGATCCTGG GCCCAAAGGG TTTGGCCCTG GATACCTCGG TGGCTTCCTC 
CTGGTTCTCC ACAGTCAGAC GGACCAGGAG CCCACCTGCC CCCTGGGCAT GCCCAGGCTC 
TGGACTGGGT ATAGTCTGTT ATACCTGGAA GGGCAAGAGA AAGCTCACAA TCAAGACCTT 
GGTCTGGCAG GGTCTTGCCT TCCCGTATTT AGCACGCTGC CCTTTGCCTA CTGCAACATC 
CACCAGGTGT GCCACTATGC CCAGAGAAAC GACAGATCCT ACTGGCTGGC CAGCGCTGCG 
CCCCTCCCCA TGATGCCACT CTCTGAAGAG GCGATCCGCC CCTATGTCAG CCGCTGTGCG 
GTATGCGAGG CCCCGGCCCA GGCGGTGGCG GTGCACAGCC AGGACCAGTC CATCCCCCCA 
TGTCCGCAGA CCTGGAGGAG CCTCTGGATC GGGTATTCAT TCCTGATGCA CACAGGAGCT 
GGGGACCAAG GAGGAGGGCA GGCCCTTATG TCACCTGGCA GCTGCCTGGA AGATTTCAGA 
GCAGCACCAT TCCTTGAATG CCAGGGCCGG CAGGGAACTT GCCACTTTTT CGCAAATAAG 
TATAGCTTCT GGCTCACAAC GGTGAAAGCA GACTTGCAGT TTTCCTCTGC TCCAGCACCA 
GACACCTTAA AAGAAAGCCA GGCCCAACGC CAGAAAATCA GCCGGTGCCA GGTCTGCGTG 
AAGTATAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510558.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508860.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.

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CloneID	OHu56972
Clone ID Related Accession (Same CDS sequence)	XM_011510558.2
Accession Version	XM_011510558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTTT TCGTGGTGAC ATGGGAGATC CGGGTTTTGG AGGTGAAAAG 
GGGTCCTCCC CTGTTGGGCC CCCAGGCCCT CCCGGCTCAC CAGGAGTGAA TGGTCAGAAA 
GGAATCCCGG GAGACCCTGC ATTTGGTCAC CTGGGACCCC CGGGAAAGAG GGGTCTTTCA 
GGAGTGCCAG GGATAAAAGG ACCCAGAGGT GATCCGGGAT GTCCAGGGGC TGAAGGGCCA 
GCTGGCATTC CTGGATTCCT AGGTCTCAAA GGTCCCAAAG GCAGAGAGGG ACATGCTGGG 
TTTCCAGGTG TCCCAGGTCC ACCTGGCCAT TCCTGTGAAA GAGGTGCTCC AGGGATACCA 
GGGCAACCGG GACTCCCTGG GTATCCAGGT AGCCCAGGTG CTCCAGGTGG GAAAGGACAG 
CCGGGAGATG TGGGGCCTCC CGGGCCAGCT GGAATGAAAG GCCTCCCCGG ACTCCCAGGA 
CGGCCTGGGG CACATGGTCC CCCAGGCCTC CCAGGAATCC CAGGTCCCTT TGGAGATGAT 
GGGCTACCTG GTCCTCCAGG TCCAAAGGGA CCCCGGGGGC TGCCTGGTTT CCCAGGTTTT 
CCCGGAGAAA GAGGAAAGCC TGGTGCAGAG GGATGTCCTG GCGCAAAGGG AGAACCTGGA 
GAGAAGGGCA TGTCTGGCCT TCCTGGAGAC CGGGGACTGA GAGGGGCCAA AGGAGCCATA 
GGACCTCCCG GAGATGAAGG AGAAATGGCT ATCATTTCAC AAAAGGGAAC ACCTGGGGAA 
CCTGGACCTC CTGGAGATGA TGGATTCCCA GGAGAAAGAG GTGATAAAGG AACTCCCGGG 
ATGCAAGGGA GAAGAGGAGA GCCGGGAAGA TACGGACCAC CTGGATTTCA CAGAGGGGAA 
CCTGGTGAGA AAGGTCAGCC AGGGCCTCCT GGACCCCCAG GCCCTCCAGG CTCAACTGGT 
CTAAGAGGGT TCATTGGTTT TCCAGGACTT CCAGGTGACC AGGGTGAGCC AGGTTCTCCA 
GGTCCCCCTG GATTTTCAGG AATTGATGGA GCAAGAGGAC CTAAAGGAAA CAAAGGTGAC 
CCTGCCAGTC ACTTTGGTCC ACCTGGTCCA AAGGGTGAGC CAGGTAGCCC TGGATGTCCA 
GGGCATTTTG GAGCATCCGG AGAGCAGGGC TTGCCTGGTA TTCAAGGGCC CAGAGGATCA 
CCTGGAAGGC CAGGGCCACC TGGCTCCTCT GGACCACCAG GGTGCCCAGG TGATCACGGG 
ATGCCTGGGC TGAGGGGACA GCCAGGAGAA ATGGGAGACC CTGGGCCAAG AGGCCTCCAG 
GGGGATCCAG GGATACCAGG TCCTCCGGGA ATAAAAGGTC CCTCCGGATC ACCTGGCCTG 
AACGGCTTGC ATGGATTGAA AGGTCAGAAA GGAACTAAAG GTGCTTCAGG TTTGCATGAT 
GTGGGGCCAC CTGGTCCAGT GGGAATACCT GGGCTAAAAG GGGAGAGAGG AGACCCTGGG 
AGCCCAGGAA TCTCTCCTCC AGGTCCTCGT GGAAAGAAAG GTCCCCCAGG ACCCCCAGGG 
AGTTCAGGAC CACCTGGTCC TGCAGGTGCC ACAGGAAGAG CTCCTAAGGA CATTCCTGAC 
CCGGGTCCAC CTGGAGATCA GGGACCTCCT GGTCCTGATG GCCCAAGAGG AGCACCTGGG 
CCTCCAGGCC TCCCTGGGAG TGTTGACCTT CTGAGAGGGG AGCCAGGTGA CTGTGGTCTA 
CCAGGGCCAC CAGGTCCCCC TGGCCCACCA GGCCCTCCAG GATACAAAGG CTTTCCAGGA 
TGTGATGGAA AAGATGGCCA GAAAGGACCA GTGGGATTCC CGGGACCGCA GGGACCACAT 
GGATTTCCTG GGCCACCTGG AGAGAAGGGT TTACCTGGAC CTCCAGGGAG AAAAGGGCCC 
ACTGGTCTTC CGGGTCCCAG AGGTGAACCG GGGCCACCTG CAGATGTGGA TGACTGTCCC 
CGAATCCCAG GCCTTCCTGG GGCGCCAGGC ATGAGAGGAC CAGAAGGAGC CATGGGGCTC 
CCTGGAATGA GAGGCCCCTC AGGACCAGGG TGCAAAGGAG AGCCTGGGCT GGATGGCAGG 
AGGGGTGTGG ATGGCGTCCC TGGGTCTCCT GGGCCTCCCG GACGTAAAGG TGACACAGGA 
GAAGACGGCT ACCCTGGAGG ACCAGGGCCT CCTGGTCCCA TTGGGGATCC TGGGCCCAAA 
GGGTTTGGCC CTGGATACCT CGGTGGCTTC CTCCTGGTTC TCCACAGTCA GACGGACCAG 
GAGCCCACCT GCCCCCTGGG CATGCCCAGG CTCTGGACTG GGTATAGTCT GTTATACCTG 
GAAGGGCAAG AGAAAGCTCA CAATCAAGAC CTTGGTCTGG CAGGGTCTTG CCTTCCCGTA 
TTTAGCACGC TGCCCTTTGC CTACTGCAAC ATCCACCAGG TGTGCCACTA TGCCCAGAGA 
AACGACAGAT CCTACTGGCT GGCCAGCGCT GCGCCCCTCC CCATGATGCC ACTCTCTGAA 
GAGGCGATCC GCCCCTATGT CAGCCGCTGT GCGGTATGCG AGGCCCCGGC CCAGGCGGTG 
GCGGTGCACA GCCAGGACCA GTCCATCCCC CCATGTCCGC AGACCTGGAG GAGCCTCTGG 
ATCGGGTATT CATTCCTGAT GCACACAGGA GCTGGGGACC AAGGAGGAGG GCAGGCCCTT 
ATGTCACCTG GCAGCTGCCT GGAAGATTTC AGAGCAGCAC CATTCCTTGA ATGCCAGGGC 
CGGCAGGGAA CTTGCCACTT TTTCGCAAAT AAGTATAGCT TCTGGCTCAC AACGGTGAAA 
GCAGACTTGC AGTTTTCCTC TGCTCCAGCA CCAGACACCT TAAAAGAAAG CCAGGCCCAA 
CGCCAGAAAA TCAGCCGGTG CCAGGTCTGC GTGAAGTATA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508860.1
CDS	327..5291
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_011510558.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510558.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTTT TCGTGGTGAC ATGGGAGATC CGGGTTTTGG AGGTGAAAAG 
GGGTCCTCCC CTGTTGGGCC CCCAGGCCCT CCCGGCTCAC CAGGAGTGAA TGGTCAGAAA 
GGAATCCCGG GAGACCCTGC ATTTGGTCAC CTGGGACCCC CGGGAAAGAG GGGTCTTTCA 
GGAGTGCCAG GGATAAAAGG ACCCAGAGGT GATCCGGGAT GTCCAGGGGC TGAAGGGCCA 
GCTGGCATTC CTGGATTCCT AGGTCTCAAA GGTCCCAAAG GCAGAGAGGG ACATGCTGGG 
TTTCCAGGTG TCCCAGGTCC ACCTGGCCAT TCCTGTGAAA GAGGTGCTCC AGGGATACCA 
GGGCAACCGG GACTCCCTGG GTATCCAGGT AGCCCAGGTG CTCCAGGTGG GAAAGGACAG 
CCGGGAGATG TGGGGCCTCC CGGGCCAGCT GGAATGAAAG GCCTCCCCGG ACTCCCAGGA 
CGGCCTGGGG CACATGGTCC CCCAGGCCTC CCAGGAATCC CAGGTCCCTT TGGAGATGAT 
GGGCTACCTG GTCCTCCAGG TCCAAAGGGA CCCCGGGGGC TGCCTGGTTT CCCAGGTTTT 
CCCGGAGAAA GAGGAAAGCC TGGTGCAGAG GGATGTCCTG GCGCAAAGGG AGAACCTGGA 
GAGAAGGGCA TGTCTGGCCT TCCTGGAGAC CGGGGACTGA GAGGGGCCAA AGGAGCCATA 
GGACCTCCCG GAGATGAAGG AGAAATGGCT ATCATTTCAC AAAAGGGAAC ACCTGGGGAA 
CCTGGACCTC CTGGAGATGA TGGATTCCCA GGAGAAAGAG GTGATAAAGG AACTCCCGGG 
ATGCAAGGGA GAAGAGGAGA GCCGGGAAGA TACGGACCAC CTGGATTTCA CAGAGGGGAA 
CCTGGTGAGA AAGGTCAGCC AGGGCCTCCT GGACCCCCAG GCCCTCCAGG CTCAACTGGT 
CTAAGAGGGT TCATTGGTTT TCCAGGACTT CCAGGTGACC AGGGTGAGCC AGGTTCTCCA 
GGTCCCCCTG GATTTTCAGG AATTGATGGA GCAAGAGGAC CTAAAGGAAA CAAAGGTGAC 
CCTGCCAGTC ACTTTGGTCC ACCTGGTCCA AAGGGTGAGC CAGGTAGCCC TGGATGTCCA 
GGGCATTTTG GAGCATCCGG AGAGCAGGGC TTGCCTGGTA TTCAAGGGCC CAGAGGATCA 
CCTGGAAGGC CAGGGCCACC TGGCTCCTCT GGACCACCAG GGTGCCCAGG TGATCACGGG 
ATGCCTGGGC TGAGGGGACA GCCAGGAGAA ATGGGAGACC CTGGGCCAAG AGGCCTCCAG 
GGGGATCCAG GGATACCAGG TCCTCCGGGA ATAAAAGGTC CCTCCGGATC ACCTGGCCTG 
AACGGCTTGC ATGGATTGAA AGGTCAGAAA GGAACTAAAG GTGCTTCAGG TTTGCATGAT 
GTGGGGCCAC CTGGTCCAGT GGGAATACCT GGGCTAAAAG GGGAGAGAGG AGACCCTGGG 
AGCCCAGGAA TCTCTCCTCC AGGTCCTCGT GGAAAGAAAG GTCCCCCAGG ACCCCCAGGG 
AGTTCAGGAC CACCTGGTCC TGCAGGTGCC ACAGGAAGAG CTCCTAAGGA CATTCCTGAC 
CCGGGTCCAC CTGGAGATCA GGGACCTCCT GGTCCTGATG GCCCAAGAGG AGCACCTGGG 
CCTCCAGGCC TCCCTGGGAG TGTTGACCTT CTGAGAGGGG AGCCAGGTGA CTGTGGTCTA 
CCAGGGCCAC CAGGTCCCCC TGGCCCACCA GGCCCTCCAG GATACAAAGG CTTTCCAGGA 
TGTGATGGAA AAGATGGCCA GAAAGGACCA GTGGGATTCC CGGGACCGCA GGGACCACAT 
GGATTTCCTG GGCCACCTGG AGAGAAGGGT TTACCTGGAC CTCCAGGGAG AAAAGGGCCC 
ACTGGTCTTC CGGGTCCCAG AGGTGAACCG GGGCCACCTG CAGATGTGGA TGACTGTCCC 
CGAATCCCAG GCCTTCCTGG GGCGCCAGGC ATGAGAGGAC CAGAAGGAGC CATGGGGCTC 
CCTGGAATGA GAGGCCCCTC AGGACCAGGG TGCAAAGGAG AGCCTGGGCT GGATGGCAGG 
AGGGGTGTGG ATGGCGTCCC TGGGTCTCCT GGGCCTCCCG GACGTAAAGG TGACACAGGA 
GAAGACGGCT ACCCTGGAGG ACCAGGGCCT CCTGGTCCCA TTGGGGATCC TGGGCCCAAA 
GGGTTTGGCC CTGGATACCT CGGTGGCTTC CTCCTGGTTC TCCACAGTCA GACGGACCAG 
GAGCCCACCT GCCCCCTGGG CATGCCCAGG CTCTGGACTG GGTATAGTCT GTTATACCTG 
GAAGGGCAAG AGAAAGCTCA CAATCAAGAC CTTGGTCTGG CAGGGTCTTG CCTTCCCGTA 
TTTAGCACGC TGCCCTTTGC CTACTGCAAC ATCCACCAGG TGTGCCACTA TGCCCAGAGA 
AACGACAGAT CCTACTGGCT GGCCAGCGCT GCGCCCCTCC CCATGATGCC ACTCTCTGAA 
GAGGCGATCC GCCCCTATGT CAGCCGCTGT GCGGTATGCG AGGCCCCGGC CCAGGCGGTG 
GCGGTGCACA GCCAGGACCA GTCCATCCCC CCATGTCCGC AGACCTGGAG GAGCCTCTGG 
ATCGGGTATT CATTCCTGAT GCACACAGGA GCTGGGGACC AAGGAGGAGG GCAGGCCCTT 
ATGTCACCTG GCAGCTGCCT GGAAGATTTC AGAGCAGCAC CATTCCTTGA ATGCCAGGGC 
CGGCAGGGAA CTTGCCACTT TTTCGCAAAT AAGTATAGCT TCTGGCTCAC AACGGTGAAA 
GCAGACTTGC AGTTTTCCTC TGCTCCAGCA CCAGACACCT TAAAAGAAAG CCAGGCCCAA 
CGCCAGAAAA TCAGCCGGTG CCAGGTCTGC GTGAAGTATA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510565.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508867.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X18, mRNA.

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CloneID	OHu56975
Clone ID Related Accession (Same CDS sequence)	XM_011510565.2
Accession Version	XM_011510565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGTGG TGAGTGCCTC TCGCTATGTT TGATGCTACA CACCTGCTGC 
CTGAGCAGCC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508867.1
CDS	327..4601
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGGECLSLCLMLHTCCLSSP

Target ORF information:

RefSeq Version	XM_011510565.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510565.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGTGG TGAGTGCCTC TCGCTATGTT TGATGCTACA CACCTGCTGC 
CTGAGCAGCC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510568.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508870.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X23, mRNA.

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CloneID	OHu56976
Clone ID Related Accession (Same CDS sequence)	XM_011510568.2
Accession Version	XM_011510568.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510568.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGTGC TGCCGCTGCT GCTGCTGGTC CAGTAACTAC TCTGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508870.1
CDS	327..4586
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGAAAAAAGPVTTLI

Target ORF information:

RefSeq Version	XM_011510568.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510568.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGTGC TGCCGCTGCT GCTGCTGGTC CAGTAACTAC TCTGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510570.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508872.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OHu56977
Clone ID Related Accession (Same CDS sequence)	XM_011510570.2 , XM_011510569.2 , XM_017003300.1
Accession Version	XM_011510570.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508872.1
CDS	327..4571
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPAHGCVLRTK

Target ORF information:

RefSeq Version	XM_011510570.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510570.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510569.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508871.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OHu56977
Clone ID Related Accession (Same CDS sequence)	XM_011510570.2 , XM_011510569.2 , XM_017003300.1
Accession Version	XM_011510569.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508871.1
CDS	327..4571
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPAHGCVLRTK

Target ORF information:

RefSeq Version	XM_011510569.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510569.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_017003300.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858789.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X24, mRNA.

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CloneID	OHu56977
Clone ID Related Accession (Same CDS sequence)	XM_011510570.2 , XM_011510569.2 , XM_017003300.1
Accession Version	XM_017003300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858789.1
CDS	327..4571
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPAHGCVLRTK

Target ORF information:

RefSeq Version	XM_017003300.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003300.1

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510572.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508874.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X27, mRNA.

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CloneID	OHu56978
Clone ID Related Accession (Same CDS sequence)	XM_011510572.3 , XM_011510572.2
Accession Version	XM_011510572.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510572.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAT CAAGAGTTAA AGGGCACAAA GGAGAAAGAG GTCCTGATGG GCCCCCAGGA 
TTTCCAGGGC AGCCAGGATC ACATGGTCGG GATGGACATG CTGGAGAAAA AGGGGATCCA 
GGACCTCCAG GGGATCATGA AGATGCGACC CCAGGTGGTA AAGGATTTCC TGGACCTCTG 
GGCCCCCCAG GCAAAGCAGG ACCTGTGGGG CCCCCAGGAC TGGGATTTCC TGGTCCACCA 
GGAGAGCGAG GCCACCCAGG AGTTCCAGGC CACCCAGGTG TGAGGGGCCC TGATGGCTTG 
AAGGGTCAGA AAGGTGACAC AATTTCTTGC AACGTAACCT ACCCTGGGAG GCATGGCCCT 
CCAGGTTTTG ATGGACCTCC AGGTCCGAAG GGATTTCCAG GTCCCCAAGG TGCCCCTGGG 
CTGAGTGGTT CAGATGGGCA TAAAGGCAGA CCTGGCACAC CAGGAACAGC GGAAATACCA 
GGTCCACCTG GTTTTCGTGG TGACATGGGA GATCCGGGTT TTGGAGGTGA AAAGGGGTCC 
TCCCCTGTTG GGCCCCCAGG CCCTCCCGGC TCACCAGGAG TGAATGGTCA GAAAGGAATC 
CCGGGAGACC CTGCATTTGG TCACCTGGGA CCCCCGGGAA AGAGGGGTCT TTCAGGAGTG 
CCAGGGATAA AAGGACCCAG AGGTGATCCG GGATGTCCAG GGGCTGAAGG GCCAGCTGGC 
ATTCCTGGAT TCCTAGGTCT CAAAGGTCCC AAAGGCAGAG AGGGACATGC TGGGTTTCCA 
GGTGTCCCAG GTCCACCTGG CCATTCCTGT GAAAGAGGTG CTCCAGGGAT ACCAGGGCAA 
CCGGGACTCC CTGGGTATCC AGGTAGCCCA GGTGCTCCAG GTGGGAAAGG ACAGCCGGGA 
GATGTGGGGC CTCCCGGGCC AGCTGGAATG AAAGGCCTCC CCGGACTCCC AGGACGGCCT 
GGGGCACATG GTCCCCCAGG CCTCCCAGGA ATCCCAGGTC CCTTTGGAGA TGATGGGCTA 
CCTGGTCCTC CAGGTCCAAA GGGACCCCGG GGGCTGCCTG GTTTCCCAGG TTTTCCCGGA 
GAAAGAGGAA AGCCTGGTGC AGAGGGATGT CCTGGCGCAA AGGGAGAACC TGGAGAGAAG 
GGCATGTCTG GCCTTCCTGG AGACCGGGGA CTGAGAGGGG CCAAAGGAGC CATAGGACCT 
CCCGGAGATG AAGGAGAAAT GGCTATCATT TCACAAAAGG GAACACCTGG GGAACCTGGA 
CCTCCTGGAG ATGATGGATT CCCAGGAGAA AGAGGTGATA AAGGAACTCC CGGGATGCAA 
GGGAGAAGAG GAGAGCCGGG AAGATACGGA CCACCTGGAT TTCACAGAGG GGAACCTGGT 
GAGAAAGGTC AGCCAGGGCC TCCTGGACCC CCAGGCCCTC CAGGCTCAAC TGGTCTAAGA 
GGGTTCATTG GTTTTCCAGG ACTTCCAGGT GACCAGGGTG AGCCAGGTTC TCCAGGTCCC 
CCTGGATTTT CAGGAATTGA TGGAGCAAGA GGACCTAAAG GAAACAAAGG TGACCCTGCC 
AGTCACTTTG GTCCACCTGG TCCAAAGGGT GAGCCAGGTA GCCCTGGATG TCCAGGGCAT 
TTTGGAGCAT CCGGAGAGCA GGGCTTGCCT GGTATTCAAG GGCCCAGAGG ATCACCTGGA 
AGGCCAGGGC CACCTGGCTC CTCTGGACCA CCAGGGTGCC CAGGTGATCA CGGGATGCCT 
GGGCTGAGGG GACAGCCAGG AGAAATGGGA GACCCTGGGC CAAGAGGCCT CCAGGGGGAT 
CCAGGGATAC CAGGTCCTCC GGGAATAAAA GGTCCCTCCG GATCACCTGG CCTGAACGGC 
TTGCATGGAT TGAAAGGTCA GAAAGGAACT AAAGGTGCTT CAGGTTTGCA TGATGTGGGG 
CCACCTGGTC CAGTGGGAAT ACCTGGGCTA AAAGGGGAGA GAGGAGACCC TGGGAGCCCA 
GGAATCTCTC CTCCAGGTCC TCGTGGAAAG AAAGGTCCCC CAGGACCCCC AGGGAGTTCA 
GGACCACCTG GTCCTGCAGG TGCCACAGGA AGAGCTCCTA AGGACATTCC TGACCCGGGT 
CCACCTGGAG ATCAGGGACC TCCTGGTCCT GATGGCCCAA GAGGAGCACC TGGGCCTCCA 
GGCCTCCCTG GGAGTGTTGA CCTTCTGAGA GGGGAGCCAG GTGACTGTGG TCTACCAGGG 
CCACCAGGTC CCCCTGGCCC ACCAGGCCCT CCAGGATACA AAGGCTTTCC AGGATGTGAT 
GGAAAAGATG GCCAGAAAGG ACCAGTGGGA TTCCCGGGAC CGCAGGGACC ACATGGATTT 
CCTGGGCCAC CTGGAGAGAA GGGTTTACCT GGACCTCCAG GGAGAAAAGG GCCCACTGGT 
CTTCCGGGTC CCAGAGGTGA ACCGGGGCCA CCTGCAGATG TGGATGACTG TCCCCGAATC 
CCAGGCCTTC CTGGGGCGCC AGGCATGAGA GGACCAGAAG GAGCCATGGG GCTCCCTGGA 
ATGAGAGGCC CCTCAGGACC AGGGTGCAAA GGAGAGCCTG GGCTGGATGG CAGGAGGGGT 
GTGGATGGCG TCCCTGGGTC TCCTGGGCCT CCCGGACGTA AAGGTGACAC AGGAGAAGAC 
GGCTACCCTG GAGGACCAGG GCCTCCTGGT CCCATTGGGG ATCCTGGGCC CAAAGGGTTT 
GGCCCTGGAT ACCTCGGTGG CTTCCTCCTG GTTCTCCACA GTCAGACGGA CCAGGAGCCC 
ACCTGCCCCC TGGGCATGCC CAGGCTCTGG ACTGGGTATA GTCTGTTATA CCTGGAAGGG 
CAAGAGAAAG CTCACAATCA AGACCTTGGT CTGGCAGGGT CTTGCCTTCC CGTATTTAGC 
ACGCTGCCCT TTGCCTACTG CAACATCCAC CAGGTGTGCC ACTATGCCCA GAGAAACGAC 
AGATCCTACT GGCTGGCCAG CGCTGCGCCC CTCCCCATGA TGCCACTCTC TGAAGAGGCG 
ATCCGCCCCT ATGTCAGCCG CTGTGCGGTA TGCGAGGCCC CGGCCCAGGC GGTGGCGGTG 
CACAGCCAGG ACCAGTCCAT CCCCCCATGT CCGCAGACCT GGAGGAGCCT CTGGATCGGG 
TATTCATTCC TGATGCACAC AGGAGCTGGG GACCAAGGAG GAGGGCAGGC CCTTATGTCA 
CCTGGCAGCT GCCTGGAAGA TTTCAGAGCA GCACCATTCC TTGAATGCCA GGGCCGGCAG 
GGAACTTGCC ACTTTTTCGC AAATAAGTAT AGCTTCTGGC TCACAACGGT GAAAGCAGAC 
TTGCAGTTTT CCTCTGCTCC AGCACCAGAC ACCTTAAAAG AAAGCCAGGC CCAACGCCAG 
AAAATCAGCC GGTGCCAGGT CTGCGTGAAG TATAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508874.1
CDS	229..3627
Translation	MVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_011510572.3
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510572.3

ORF Insert Sequence:

ATGGTTGTAT CAAGAGTTAA AGGGCACAAA GGAGAAAGAG GTCCTGATGG GCCCCCAGGA 
TTTCCAGGGC AGCCAGGATC ACATGGTCGG GATGGACATG CTGGAGAAAA AGGGGATCCA 
GGACCTCCAG GGGATCATGA AGATGCGACC CCAGGTGGTA AAGGATTTCC TGGACCTCTG 
GGCCCCCCAG GCAAAGCAGG ACCTGTGGGG CCCCCAGGAC TGGGATTTCC TGGTCCACCA 
GGAGAGCGAG GCCACCCAGG AGTTCCAGGC CACCCAGGTG TGAGGGGCCC TGATGGCTTG 
AAGGGTCAGA AAGGTGACAC AATTTCTTGC AACGTAACCT ACCCTGGGAG GCATGGCCCT 
CCAGGTTTTG ATGGACCTCC AGGTCCGAAG GGATTTCCAG GTCCCCAAGG TGCCCCTGGG 
CTGAGTGGTT CAGATGGGCA TAAAGGCAGA CCTGGCACAC CAGGAACAGC GGAAATACCA 
GGTCCACCTG GTTTTCGTGG TGACATGGGA GATCCGGGTT TTGGAGGTGA AAAGGGGTCC 
TCCCCTGTTG GGCCCCCAGG CCCTCCCGGC TCACCAGGAG TGAATGGTCA GAAAGGAATC 
CCGGGAGACC CTGCATTTGG TCACCTGGGA CCCCCGGGAA AGAGGGGTCT TTCAGGAGTG 
CCAGGGATAA AAGGACCCAG AGGTGATCCG GGATGTCCAG GGGCTGAAGG GCCAGCTGGC 
ATTCCTGGAT TCCTAGGTCT CAAAGGTCCC AAAGGCAGAG AGGGACATGC TGGGTTTCCA 
GGTGTCCCAG GTCCACCTGG CCATTCCTGT GAAAGAGGTG CTCCAGGGAT ACCAGGGCAA 
CCGGGACTCC CTGGGTATCC AGGTAGCCCA GGTGCTCCAG GTGGGAAAGG ACAGCCGGGA 
GATGTGGGGC CTCCCGGGCC AGCTGGAATG AAAGGCCTCC CCGGACTCCC AGGACGGCCT 
GGGGCACATG GTCCCCCAGG CCTCCCAGGA ATCCCAGGTC CCTTTGGAGA TGATGGGCTA 
CCTGGTCCTC CAGGTCCAAA GGGACCCCGG GGGCTGCCTG GTTTCCCAGG TTTTCCCGGA 
GAAAGAGGAA AGCCTGGTGC AGAGGGATGT CCTGGCGCAA AGGGAGAACC TGGAGAGAAG 
GGCATGTCTG GCCTTCCTGG AGACCGGGGA CTGAGAGGGG CCAAAGGAGC CATAGGACCT 
CCCGGAGATG AAGGAGAAAT GGCTATCATT TCACAAAAGG GAACACCTGG GGAACCTGGA 
CCTCCTGGAG ATGATGGATT CCCAGGAGAA AGAGGTGATA AAGGAACTCC CGGGATGCAA 
GGGAGAAGAG GAGAGCCGGG AAGATACGGA CCACCTGGAT TTCACAGAGG GGAACCTGGT 
GAGAAAGGTC AGCCAGGGCC TCCTGGACCC CCAGGCCCTC CAGGCTCAAC TGGTCTAAGA 
GGGTTCATTG GTTTTCCAGG ACTTCCAGGT GACCAGGGTG AGCCAGGTTC TCCAGGTCCC 
CCTGGATTTT CAGGAATTGA TGGAGCAAGA GGACCTAAAG GAAACAAAGG TGACCCTGCC 
AGTCACTTTG GTCCACCTGG TCCAAAGGGT GAGCCAGGTA GCCCTGGATG TCCAGGGCAT 
TTTGGAGCAT CCGGAGAGCA GGGCTTGCCT GGTATTCAAG GGCCCAGAGG ATCACCTGGA 
AGGCCAGGGC CACCTGGCTC CTCTGGACCA CCAGGGTGCC CAGGTGATCA CGGGATGCCT 
GGGCTGAGGG GACAGCCAGG AGAAATGGGA GACCCTGGGC CAAGAGGCCT CCAGGGGGAT 
CCAGGGATAC CAGGTCCTCC GGGAATAAAA GGTCCCTCCG GATCACCTGG CCTGAACGGC 
TTGCATGGAT TGAAAGGTCA GAAAGGAACT AAAGGTGCTT CAGGTTTGCA TGATGTGGGG 
CCACCTGGTC CAGTGGGAAT ACCTGGGCTA AAAGGGGAGA GAGGAGACCC TGGGAGCCCA 
GGAATCTCTC CTCCAGGTCC TCGTGGAAAG AAAGGTCCCC CAGGACCCCC AGGGAGTTCA 
GGACCACCTG GTCCTGCAGG TGCCACAGGA AGAGCTCCTA AGGACATTCC TGACCCGGGT 
CCACCTGGAG ATCAGGGACC TCCTGGTCCT GATGGCCCAA GAGGAGCACC TGGGCCTCCA 
GGCCTCCCTG GGAGTGTTGA CCTTCTGAGA GGGGAGCCAG GTGACTGTGG TCTACCAGGG 
CCACCAGGTC CCCCTGGCCC ACCAGGCCCT CCAGGATACA AAGGCTTTCC AGGATGTGAT 
GGAAAAGATG GCCAGAAAGG ACCAGTGGGA TTCCCGGGAC CGCAGGGACC ACATGGATTT 
CCTGGGCCAC CTGGAGAGAA GGGTTTACCT GGACCTCCAG GGAGAAAAGG GCCCACTGGT 
CTTCCGGGTC CCAGAGGTGA ACCGGGGCCA CCTGCAGATG TGGATGACTG TCCCCGAATC 
CCAGGCCTTC CTGGGGCGCC AGGCATGAGA GGACCAGAAG GAGCCATGGG GCTCCCTGGA 
ATGAGAGGCC CCTCAGGACC AGGGTGCAAA GGAGAGCCTG GGCTGGATGG CAGGAGGGGT 
GTGGATGGCG TCCCTGGGTC TCCTGGGCCT CCCGGACGTA AAGGTGACAC AGGAGAAGAC 
GGCTACCCTG GAGGACCAGG GCCTCCTGGT CCCATTGGGG ATCCTGGGCC CAAAGGGTTT 
GGCCCTGGAT ACCTCGGTGG CTTCCTCCTG GTTCTCCACA GTCAGACGGA CCAGGAGCCC 
ACCTGCCCCC TGGGCATGCC CAGGCTCTGG ACTGGGTATA GTCTGTTATA CCTGGAAGGG 
CAAGAGAAAG CTCACAATCA AGACCTTGGT CTGGCAGGGT CTTGCCTTCC CGTATTTAGC 
ACGCTGCCCT TTGCCTACTG CAACATCCAC CAGGTGTGCC ACTATGCCCA GAGAAACGAC 
AGATCCTACT GGCTGGCCAG CGCTGCGCCC CTCCCCATGA TGCCACTCTC TGAAGAGGCG 
ATCCGCCCCT ATGTCAGCCG CTGTGCGGTA TGCGAGGCCC CGGCCCAGGC GGTGGCGGTG 
CACAGCCAGG ACCAGTCCAT CCCCCCATGT CCGCAGACCT GGAGGAGCCT CTGGATCGGG 
TATTCATTCC TGATGCACAC AGGAGCTGGG GACCAAGGAG GAGGGCAGGC CCTTATGTCA 
CCTGGCAGCT GCCTGGAAGA TTTCAGAGCA GCACCATTCC TTGAATGCCA GGGCCGGCAG 
GGAACTTGCC ACTTTTTCGC AAATAAGTAT AGCTTCTGGC TCACAACGGT GAAAGCAGAC 
TTGCAGTTTT CCTCTGCTCC AGCACCAGAC ACCTTAAAAG AAAGCCAGGC CCAACGCCAG 
AAAATCAGCC GGTGCCAGGT CTGCGTGAAG TATAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_011510572.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508874.1 Homo sapiens collagen type IV alpha 4 (COL4A4), transcript variant X28, mRNA.

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CloneID	OHu56978
Clone ID Related Accession (Same CDS sequence)	XM_011510572.3 , XM_011510572.2
Accession Version	XM_011510572.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510572.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGTAT CAAGAGTTAA AGGGCACAAA GGAGAAAGAG GTCCTGATGG GCCCCCAGGA 
TTTCCAGGGC AGCCAGGATC ACATGGTCGG GATGGACATG CTGGAGAAAA AGGGGATCCA 
GGACCTCCAG GGGATCATGA AGATGCGACC CCAGGTGGTA AAGGATTTCC TGGACCTCTG 
GGCCCCCCAG GCAAAGCAGG ACCTGTGGGG CCCCCAGGAC TGGGATTTCC TGGTCCACCA 
GGAGAGCGAG GCCACCCAGG AGTTCCAGGC CACCCAGGTG TGAGGGGCCC TGATGGCTTG 
AAGGGTCAGA AAGGTGACAC AATTTCTTGC AACGTAACCT ACCCTGGGAG GCATGGCCCT 
CCAGGTTTTG ATGGACCTCC AGGTCCGAAG GGATTTCCAG GTCCCCAAGG TGCCCCTGGG 
CTGAGTGGTT CAGATGGGCA TAAAGGCAGA CCTGGCACAC CAGGAACAGC GGAAATACCA 
GGTCCACCTG GTTTTCGTGG TGACATGGGA GATCCGGGTT TTGGAGGTGA AAAGGGGTCC 
TCCCCTGTTG GGCCCCCAGG CCCTCCCGGC TCACCAGGAG TGAATGGTCA GAAAGGAATC 
CCGGGAGACC CTGCATTTGG TCACCTGGGA CCCCCGGGAA AGAGGGGTCT TTCAGGAGTG 
CCAGGGATAA AAGGACCCAG AGGTGATCCG GGATGTCCAG GGGCTGAAGG GCCAGCTGGC 
ATTCCTGGAT TCCTAGGTCT CAAAGGTCCC AAAGGCAGAG AGGGACATGC TGGGTTTCCA 
GGTGTCCCAG GTCCACCTGG CCATTCCTGT GAAAGAGGTG CTCCAGGGAT ACCAGGGCAA 
CCGGGACTCC CTGGGTATCC AGGTAGCCCA GGTGCTCCAG GTGGGAAAGG ACAGCCGGGA 
GATGTGGGGC CTCCCGGGCC AGCTGGAATG AAAGGCCTCC CCGGACTCCC AGGACGGCCT 
GGGGCACATG GTCCCCCAGG CCTCCCAGGA ATCCCAGGTC CCTTTGGAGA TGATGGGCTA 
CCTGGTCCTC CAGGTCCAAA GGGACCCCGG GGGCTGCCTG GTTTCCCAGG TTTTCCCGGA 
GAAAGAGGAA AGCCTGGTGC AGAGGGATGT CCTGGCGCAA AGGGAGAACC TGGAGAGAAG 
GGCATGTCTG GCCTTCCTGG AGACCGGGGA CTGAGAGGGG CCAAAGGAGC CATAGGACCT 
CCCGGAGATG AAGGAGAAAT GGCTATCATT TCACAAAAGG GAACACCTGG GGAACCTGGA 
CCTCCTGGAG ATGATGGATT CCCAGGAGAA AGAGGTGATA AAGGAACTCC CGGGATGCAA 
GGGAGAAGAG GAGAGCCGGG AAGATACGGA CCACCTGGAT TTCACAGAGG GGAACCTGGT 
GAGAAAGGTC AGCCAGGGCC TCCTGGACCC CCAGGCCCTC CAGGCTCAAC TGGTCTAAGA 
GGGTTCATTG GTTTTCCAGG ACTTCCAGGT GACCAGGGTG AGCCAGGTTC TCCAGGTCCC 
CCTGGATTTT CAGGAATTGA TGGAGCAAGA GGACCTAAAG GAAACAAAGG TGACCCTGCC 
AGTCACTTTG GTCCACCTGG TCCAAAGGGT GAGCCAGGTA GCCCTGGATG TCCAGGGCAT 
TTTGGAGCAT CCGGAGAGCA GGGCTTGCCT GGTATTCAAG GGCCCAGAGG ATCACCTGGA 
AGGCCAGGGC CACCTGGCTC CTCTGGACCA CCAGGGTGCC CAGGTGATCA CGGGATGCCT 
GGGCTGAGGG GACAGCCAGG AGAAATGGGA GACCCTGGGC CAAGAGGCCT CCAGGGGGAT 
CCAGGGATAC CAGGTCCTCC GGGAATAAAA GGTCCCTCCG GATCACCTGG CCTGAACGGC 
TTGCATGGAT TGAAAGGTCA GAAAGGAACT AAAGGTGCTT CAGGTTTGCA TGATGTGGGG 
CCACCTGGTC CAGTGGGAAT ACCTGGGCTA AAAGGGGAGA GAGGAGACCC TGGGAGCCCA 
GGAATCTCTC CTCCAGGTCC TCGTGGAAAG AAAGGTCCCC CAGGACCCCC AGGGAGTTCA 
GGACCACCTG GTCCTGCAGG TGCCACAGGA AGAGCTCCTA AGGACATTCC TGACCCGGGT 
CCACCTGGAG ATCAGGGACC TCCTGGTCCT GATGGCCCAA GAGGAGCACC TGGGCCTCCA 
GGCCTCCCTG GGAGTGTTGA CCTTCTGAGA GGGGAGCCAG GTGACTGTGG TCTACCAGGG 
CCACCAGGTC CCCCTGGCCC ACCAGGCCCT CCAGGATACA AAGGCTTTCC AGGATGTGAT 
GGAAAAGATG GCCAGAAAGG ACCAGTGGGA TTCCCGGGAC CGCAGGGACC ACATGGATTT 
CCTGGGCCAC CTGGAGAGAA GGGTTTACCT GGACCTCCAG GGAGAAAAGG GCCCACTGGT 
CTTCCGGGTC CCAGAGGTGA ACCGGGGCCA CCTGCAGATG TGGATGACTG TCCCCGAATC 
CCAGGCCTTC CTGGGGCGCC AGGCATGAGA GGACCAGAAG GAGCCATGGG GCTCCCTGGA 
ATGAGAGGCC CCTCAGGACC AGGGTGCAAA GGAGAGCCTG GGCTGGATGG CAGGAGGGGT 
GTGGATGGCG TCCCTGGGTC TCCTGGGCCT CCCGGACGTA AAGGTGACAC AGGAGAAGAC 
GGCTACCCTG GAGGACCAGG GCCTCCTGGT CCCATTGGGG ATCCTGGGCC CAAAGGGTTT 
GGCCCTGGAT ACCTCGGTGG CTTCCTCCTG GTTCTCCACA GTCAGACGGA CCAGGAGCCC 
ACCTGCCCCC TGGGCATGCC CAGGCTCTGG ACTGGGTATA GTCTGTTATA CCTGGAAGGG 
CAAGAGAAAG CTCACAATCA AGACCTTGGT CTGGCAGGGT CTTGCCTTCC CGTATTTAGC 
ACGCTGCCCT TTGCCTACTG CAACATCCAC CAGGTGTGCC ACTATGCCCA GAGAAACGAC 
AGATCCTACT GGCTGGCCAG CGCTGCGCCC CTCCCCATGA TGCCACTCTC TGAAGAGGCG 
ATCCGCCCCT ATGTCAGCCG CTGTGCGGTA TGCGAGGCCC CGGCCCAGGC GGTGGCGGTG 
CACAGCCAGG ACCAGTCCAT CCCCCCATGT CCGCAGACCT GGAGGAGCCT CTGGATCGGG 
TATTCATTCC TGATGCACAC AGGAGCTGGG GACCAAGGAG GAGGGCAGGC CCTTATGTCA 
CCTGGCAGCT GCCTGGAAGA TTTCAGAGCA GCACCATTCC TTGAATGCCA GGGCCGGCAG 
GGAACTTGCC ACTTTTTCGC AAATAAGTAT AGCTTCTGGC TCACAACGGT GAAAGCAGAC 
TTGCAGTTTT CCTCTGCTCC AGCACCAGAC ACCTTAAAAG AAAGCCAGGC CCAACGCCAG 
AAAATCAGCC GGTGCCAGGT CTGCGTGAAG TATAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508874.1
CDS	226..3624
Translation	MVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_011510572.2
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 (COL4A4), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510572.2

ORF Insert Sequence:

ATGGTTGTAT CAAGAGTTAA AGGGCACAAA GGAGAAAGAG GTCCTGATGG GCCCCCAGGA 
TTTCCAGGGC AGCCAGGATC ACATGGTCGG GATGGACATG CTGGAGAAAA AGGGGATCCA 
GGACCTCCAG GGGATCATGA AGATGCGACC CCAGGTGGTA AAGGATTTCC TGGACCTCTG 
GGCCCCCCAG GCAAAGCAGG ACCTGTGGGG CCCCCAGGAC TGGGATTTCC TGGTCCACCA 
GGAGAGCGAG GCCACCCAGG AGTTCCAGGC CACCCAGGTG TGAGGGGCCC TGATGGCTTG 
AAGGGTCAGA AAGGTGACAC AATTTCTTGC AACGTAACCT ACCCTGGGAG GCATGGCCCT 
CCAGGTTTTG ATGGACCTCC AGGTCCGAAG GGATTTCCAG GTCCCCAAGG TGCCCCTGGG 
CTGAGTGGTT CAGATGGGCA TAAAGGCAGA CCTGGCACAC CAGGAACAGC GGAAATACCA 
GGTCCACCTG GTTTTCGTGG TGACATGGGA GATCCGGGTT TTGGAGGTGA AAAGGGGTCC 
TCCCCTGTTG GGCCCCCAGG CCCTCCCGGC TCACCAGGAG TGAATGGTCA GAAAGGAATC 
CCGGGAGACC CTGCATTTGG TCACCTGGGA CCCCCGGGAA AGAGGGGTCT TTCAGGAGTG 
CCAGGGATAA AAGGACCCAG AGGTGATCCG GGATGTCCAG GGGCTGAAGG GCCAGCTGGC 
ATTCCTGGAT TCCTAGGTCT CAAAGGTCCC AAAGGCAGAG AGGGACATGC TGGGTTTCCA 
GGTGTCCCAG GTCCACCTGG CCATTCCTGT GAAAGAGGTG CTCCAGGGAT ACCAGGGCAA 
CCGGGACTCC CTGGGTATCC AGGTAGCCCA GGTGCTCCAG GTGGGAAAGG ACAGCCGGGA 
GATGTGGGGC CTCCCGGGCC AGCTGGAATG AAAGGCCTCC CCGGACTCCC AGGACGGCCT 
GGGGCACATG GTCCCCCAGG CCTCCCAGGA ATCCCAGGTC CCTTTGGAGA TGATGGGCTA 
CCTGGTCCTC CAGGTCCAAA GGGACCCCGG GGGCTGCCTG GTTTCCCAGG TTTTCCCGGA 
GAAAGAGGAA AGCCTGGTGC AGAGGGATGT CCTGGCGCAA AGGGAGAACC TGGAGAGAAG 
GGCATGTCTG GCCTTCCTGG AGACCGGGGA CTGAGAGGGG CCAAAGGAGC CATAGGACCT 
CCCGGAGATG AAGGAGAAAT GGCTATCATT TCACAAAAGG GAACACCTGG GGAACCTGGA 
CCTCCTGGAG ATGATGGATT CCCAGGAGAA AGAGGTGATA AAGGAACTCC CGGGATGCAA 
GGGAGAAGAG GAGAGCCGGG AAGATACGGA CCACCTGGAT TTCACAGAGG GGAACCTGGT 
GAGAAAGGTC AGCCAGGGCC TCCTGGACCC CCAGGCCCTC CAGGCTCAAC TGGTCTAAGA 
GGGTTCATTG GTTTTCCAGG ACTTCCAGGT GACCAGGGTG AGCCAGGTTC TCCAGGTCCC 
CCTGGATTTT CAGGAATTGA TGGAGCAAGA GGACCTAAAG GAAACAAAGG TGACCCTGCC 
AGTCACTTTG GTCCACCTGG TCCAAAGGGT GAGCCAGGTA GCCCTGGATG TCCAGGGCAT 
TTTGGAGCAT CCGGAGAGCA GGGCTTGCCT GGTATTCAAG GGCCCAGAGG ATCACCTGGA 
AGGCCAGGGC CACCTGGCTC CTCTGGACCA CCAGGGTGCC CAGGTGATCA CGGGATGCCT 
GGGCTGAGGG GACAGCCAGG AGAAATGGGA GACCCTGGGC CAAGAGGCCT CCAGGGGGAT 
CCAGGGATAC CAGGTCCTCC GGGAATAAAA GGTCCCTCCG GATCACCTGG CCTGAACGGC 
TTGCATGGAT TGAAAGGTCA GAAAGGAACT AAAGGTGCTT CAGGTTTGCA TGATGTGGGG 
CCACCTGGTC CAGTGGGAAT ACCTGGGCTA AAAGGGGAGA GAGGAGACCC TGGGAGCCCA 
GGAATCTCTC CTCCAGGTCC TCGTGGAAAG AAAGGTCCCC CAGGACCCCC AGGGAGTTCA 
GGACCACCTG GTCCTGCAGG TGCCACAGGA AGAGCTCCTA AGGACATTCC TGACCCGGGT 
CCACCTGGAG ATCAGGGACC TCCTGGTCCT GATGGCCCAA GAGGAGCACC TGGGCCTCCA 
GGCCTCCCTG GGAGTGTTGA CCTTCTGAGA GGGGAGCCAG GTGACTGTGG TCTACCAGGG 
CCACCAGGTC CCCCTGGCCC ACCAGGCCCT CCAGGATACA AAGGCTTTCC AGGATGTGAT 
GGAAAAGATG GCCAGAAAGG ACCAGTGGGA TTCCCGGGAC CGCAGGGACC ACATGGATTT 
CCTGGGCCAC CTGGAGAGAA GGGTTTACCT GGACCTCCAG GGAGAAAAGG GCCCACTGGT 
CTTCCGGGTC CCAGAGGTGA ACCGGGGCCA CCTGCAGATG TGGATGACTG TCCCCGAATC 
CCAGGCCTTC CTGGGGCGCC AGGCATGAGA GGACCAGAAG GAGCCATGGG GCTCCCTGGA 
ATGAGAGGCC CCTCAGGACC AGGGTGCAAA GGAGAGCCTG GGCTGGATGG CAGGAGGGGT 
GTGGATGGCG TCCCTGGGTC TCCTGGGCCT CCCGGACGTA AAGGTGACAC AGGAGAAGAC 
GGCTACCCTG GAGGACCAGG GCCTCCTGGT CCCATTGGGG ATCCTGGGCC CAAAGGGTTT 
GGCCCTGGAT ACCTCGGTGG CTTCCTCCTG GTTCTCCACA GTCAGACGGA CCAGGAGCCC 
ACCTGCCCCC TGGGCATGCC CAGGCTCTGG ACTGGGTATA GTCTGTTATA CCTGGAAGGG 
CAAGAGAAAG CTCACAATCA AGACCTTGGT CTGGCAGGGT CTTGCCTTCC CGTATTTAGC 
ACGCTGCCCT TTGCCTACTG CAACATCCAC CAGGTGTGCC ACTATGCCCA GAGAAACGAC 
AGATCCTACT GGCTGGCCAG CGCTGCGCCC CTCCCCATGA TGCCACTCTC TGAAGAGGCG 
ATCCGCCCCT ATGTCAGCCG CTGTGCGGTA TGCGAGGCCC CGGCCCAGGC GGTGGCGGTG 
CACAGCCAGG ACCAGTCCAT CCCCCCATGT CCGCAGACCT GGAGGAGCCT CTGGATCGGG 
TATTCATTCC TGATGCACAC AGGAGCTGGG GACCAAGGAG GAGGGCAGGC CCTTATGTCA 
CCTGGCAGCT GCCTGGAAGA TTTCAGAGCA GCACCATTCC TTGAATGCCA GGGCCGGCAG 
GGAACTTGCC ACTTTTTCGC AAATAAGTAT AGCTTCTGGC TCACAACGGT GAAAGCAGAC 
TTGCAGTTTT CCTCTGCTCC AGCACCAGAC ACCTTAAAAG AAAGCCAGGC CCAACGCCAG 
AAAATCAGCC GGTGCCAGGT CTGCGTGAAG TATAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_017003296.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858785.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

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CloneID	OHu88997
Clone ID Related Accession (Same CDS sequence)	XM_017003296.1
Accession Version	XM_017003296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGG TGAGTCCCGT GCCCTTGAAA ACCCTGGCTC ATTCACCATT TCCTAACACC 
TTCTCCCGCC ATTTCTCTCT GGATTGGACA GTAATTCTGA GCACTCTGCG CTTAGGACAT 
AAGGTTACGG GGACTGTTGC AAACCATAGC CTGTTGGGAT GCAGGGCCAT TATCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858785.1
CDS	327..5306
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMVSPVPLKTLAHSPFPNTFSRHFSLDWTVILSTLRLGHKVTGTVANHSLLGCRAIIF

Target ORF information:

RefSeq Version	XM_017003296.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003296.1

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTATT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGG TGAGTCCCGT GCCCTTGAAA ACCCTGGCTC ATTCACCATT TCCTAACACC 
TTCTCCCGCC ATTTCTCTCT GGATTGGACA GTAATTCTGA GCACTCTGCG CTTAGGACAT 
AAGGTTACGG GGACTGTTGC AAACCATAGC CTGTTGGGAT GCAGGGCCAT TATCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_017003297.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858786.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X5, mRNA.

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CloneID	OHu88998
Clone ID Related Accession (Same CDS sequence)	XM_017003297.1
Accession Version	XM_017003297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGGCCAC CTGGCTCCTC TGGACCACCA GGGTGCCCAG GTGATCACGG GATGCCTGGG 
CTGAGGGGAC AGCCAGGAGA AATGGGAGAC CCTGGGCCAA GAGGCCTCCA GGGGGATCCA 
GGGATACCAG GTCCTCCGGG AATAAAAGGT CCCTCCGGAT CACCTGGCCT GAACGGCTTG 
CATGGATTGA AAGGTCAGAA AGGAACTAAA GGTGCTTCAG GTTTGCATGA TGTGGGGCCA 
CCTGGTCCAG TGGGAATACC TGGGCTAAAA GGGGAGAGAG GAGACCCTGG GAGCCCAGGA 
ATCTCTCCTC CAGGTCCTCG TGGAAAGAAA GGTCCCCCAG GACCCCCAGG GAGTTCAGGA 
CCACCTGGTC CTGCAGGTGC CACAGGAAGA GCTCCTAAGG ACATTCCTGA CCCGGGTCCA 
CCTGGAGATC AGGGACCTCC TGGTCCTGAT GGCCCAAGAG GAGCACCTGG GCCTCCAGGC 
CTCCCTGGGA GTGTTGACCT TCTGAGAGGG GAGCCAGGTG ACTGTGGTCT ACCAGGGCCA 
CCAGGTCCCC CTGGCCCACC AGGCCCTCCA GGATACAAAG GCTTTCCAGG ATGTGATGGA 
AAAGATGGCC AGAAAGGACC AGTGGGATTC CCGGGACCGC AGGGACCACA TGGATTTCCT 
GGGCCACCTG GAGAGAAGGG TTTACCTGGA CCTCCAGGGA GAAAAGGGCC CACTGGTCTT 
CCGGGTCCCA GAGGTGAACC GGGGCCACCT GCAGATGTGG ATGACTGTCC CCGAATCCCA 
GGCCTTCCTG GGGCGCCAGG CATGAGAGGA CCAGAAGGAG CCATGGGGCT CCCTGGAATG 
AGAGGCCCCT CAGGACCAGG GTGCAAAGGA GAGCCTGGGC TGGATGGCAG GAGGGGTGTG 
GATGGCGTCC CTGGGTCTCC TGGGCCTCCC GGACGTAAAG GTGACACAGG AGAAGACGGC 
TACCCTGGAG GACCAGGGCC TCCTGGTCCC ATTGGGGATC CTGGGCCCAA AGGGTTTGGC 
CCTGGATACC TCGGTGGCTT CCTCCTGGTT CTCCACAGTC AGACGGACCA GGAGCCCACC 
TGCCCCCTGG GCATGCCCAG GCTCTGGACT GGGTATAGTC TGTTATACCT GGAAGGGCAA 
GAGAAAGCTC ACAATCAAGA CCTTGGTCTG GCAGGGTCTT GCCTTCCCGT ATTTAGCACG 
CTGCCCTTTG CCTACTGCAA CATCCACCAG GTGTGCCACT ATGCCCAGAG AAACGACAGA 
TCCTACTGGC TGGCCAGCGC TGCGCCCCTC CCCATGATGC CACTCTCTGA AGAGGCGATC 
CGCCCCTATG TCAGCCGCTG TGCGGTATGC GAGGCCCCGG CCCAGGCGGT GGCGGTGCAC 
AGCCAGGACC AGTCCATCCC CCCATGTCCG CAGACCTGGA GGAGCCTCTG GATCGGGTAT 
TCATTCCTGA TGCACACAGG AGCTGGGGAC CAAGGAGGAG GGCAGGCCCT TATGTCACCT 
GGCAGCTGCC TGGAAGATTT CAGAGCAGCA CCATTCCTTG AATGCCAGGG CCGGCAGGGA 
ACTTGCCACT TTTTCGCAAA TAAGTATAGC TTCTGGCTCA CAACGGTGAA AGCAGACTTG 
CAGTTTTCCT CTGCTCCAGC ACCAGACACC TTAAAAGAAA GCCAGGCCCA ACGCCAGAAA 
ATCAGCCGGT GCCAGGTCTG CGTGAAGTAT AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858786.1
CDS	327..5282
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_017003297.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003297.1

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGGCCAC CTGGCTCCTC TGGACCACCA GGGTGCCCAG GTGATCACGG GATGCCTGGG 
CTGAGGGGAC AGCCAGGAGA AATGGGAGAC CCTGGGCCAA GAGGCCTCCA GGGGGATCCA 
GGGATACCAG GTCCTCCGGG AATAAAAGGT CCCTCCGGAT CACCTGGCCT GAACGGCTTG 
CATGGATTGA AAGGTCAGAA AGGAACTAAA GGTGCTTCAG GTTTGCATGA TGTGGGGCCA 
CCTGGTCCAG TGGGAATACC TGGGCTAAAA GGGGAGAGAG GAGACCCTGG GAGCCCAGGA 
ATCTCTCCTC CAGGTCCTCG TGGAAAGAAA GGTCCCCCAG GACCCCCAGG GAGTTCAGGA 
CCACCTGGTC CTGCAGGTGC CACAGGAAGA GCTCCTAAGG ACATTCCTGA CCCGGGTCCA 
CCTGGAGATC AGGGACCTCC TGGTCCTGAT GGCCCAAGAG GAGCACCTGG GCCTCCAGGC 
CTCCCTGGGA GTGTTGACCT TCTGAGAGGG GAGCCAGGTG ACTGTGGTCT ACCAGGGCCA 
CCAGGTCCCC CTGGCCCACC AGGCCCTCCA GGATACAAAG GCTTTCCAGG ATGTGATGGA 
AAAGATGGCC AGAAAGGACC AGTGGGATTC CCGGGACCGC AGGGACCACA TGGATTTCCT 
GGGCCACCTG GAGAGAAGGG TTTACCTGGA CCTCCAGGGA GAAAAGGGCC CACTGGTCTT 
CCGGGTCCCA GAGGTGAACC GGGGCCACCT GCAGATGTGG ATGACTGTCC CCGAATCCCA 
GGCCTTCCTG GGGCGCCAGG CATGAGAGGA CCAGAAGGAG CCATGGGGCT CCCTGGAATG 
AGAGGCCCCT CAGGACCAGG GTGCAAAGGA GAGCCTGGGC TGGATGGCAG GAGGGGTGTG 
GATGGCGTCC CTGGGTCTCC TGGGCCTCCC GGACGTAAAG GTGACACAGG AGAAGACGGC 
TACCCTGGAG GACCAGGGCC TCCTGGTCCC ATTGGGGATC CTGGGCCCAA AGGGTTTGGC 
CCTGGATACC TCGGTGGCTT CCTCCTGGTT CTCCACAGTC AGACGGACCA GGAGCCCACC 
TGCCCCCTGG GCATGCCCAG GCTCTGGACT GGGTATAGTC TGTTATACCT GGAAGGGCAA 
GAGAAAGCTC ACAATCAAGA CCTTGGTCTG GCAGGGTCTT GCCTTCCCGT ATTTAGCACG 
CTGCCCTTTG CCTACTGCAA CATCCACCAG GTGTGCCACT ATGCCCAGAG AAACGACAGA 
TCCTACTGGC TGGCCAGCGC TGCGCCCCTC CCCATGATGC CACTCTCTGA AGAGGCGATC 
CGCCCCTATG TCAGCCGCTG TGCGGTATGC GAGGCCCCGG CCCAGGCGGT GGCGGTGCAC 
AGCCAGGACC AGTCCATCCC CCCATGTCCG CAGACCTGGA GGAGCCTCTG GATCGGGTAT 
TCATTCCTGA TGCACACAGG AGCTGGGGAC CAAGGAGGAG GGCAGGCCCT TATGTCACCT 
GGCAGCTGCC TGGAAGATTT CAGAGCAGCA CCATTCCTTG AATGCCAGGG CCGGCAGGGA 
ACTTGCCACT TTTTCGCAAA TAAGTATAGC TTCTGGCTCA CAACGGTGAA AGCAGACTTG 
CAGTTTTCCT CTGCTCCAGC ACCAGACACC TTAAAAGAAA GCCAGGCCCA ACGCCAGAAA 
ATCAGCCGGT GCCAGGTCTG CGTGAAGTAT AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_017003299.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858788.1 Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X15, mRNA.

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CloneID	OHu88999
Clone ID Related Accession (Same CDS sequence)	XM_017003299.1
Accession Version	XM_017003299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	collagen alpha-4(IV) chain isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTATGGAT ACAGGATCGC CCATCTCCAT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858788.1
CDS	327..4883
Translation	MWSLHIVLMRCSFRLTKSLATGPWSLILILFSVQYVYGSGKKYIGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGPKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPGLGFPGPPGERGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLPGRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFHRGEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGKKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGMDTGSPISIA

Target ORF information:

RefSeq Version	XM_017003299.1
Organism	Homo sapiens(human)
Definition	Homo sapiens collagen type IV alpha 4 chain (COL4A4), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003299.1

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGGTCCCT GGTCACTTAT ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACA TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT CCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGTGCAG GAGGACCGGC AGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTA 
GAGCCACCTG ACTTTTGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG GTATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ACCCCGGACC ACAAGGAATA AAAGGCAAAG TTGGTCCCCC AGGAGGAAGA 
GGCCCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCT 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCAGGCAAAG CAGGACCTGT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGAGAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCATGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GTTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCTAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCC GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCCGGAC TCCCAGGACG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCGGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC GCAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CTGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCACAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCACA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCCTCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCCGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AAAGAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAGT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCG 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCCC TGGAATGAGA 
GGCCCCTCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTATGGAT ACAGGATCGC CCATCTCCAT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	COL4A4
Entrez Gene ID	1286
Full Name	collagen type IV alpha 4 chain
Synonyms	CA44
General protein information	Preferred Names collagen type IV alpha 4 chain Names collagen alpha-4(IV) chain Collagen IV, alpha-4 polypeptide collagen of basement membrane, alpha-4 chain collagen, type IV, alpha 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q36.3
Summary	This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. [provided by RefSeq, Jul 2008].
Disorder MIM:	120131
Disorder Html:	Alport syndrome, autosomal recessive, 203780 (3); Hematuria, familial benign (3)

Pan troglodytes (chimpanzee)	COL4A4	XP_516128.2
	COL4A4	XP_001110249.1
Canis lupus familiaris (dog)	COL4A4	NP_001026988.1
Homo sapiens (human)	COL4A4	NP_000083.3
Mus musculus (house mouse)	Col4a4	NP_031761.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

COL4A4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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