ADH5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADH5
Entrez Gene ID	128
Full Name	alcohol dehydrogenase 5 (class III), chi polypeptide
Synonyms	ADH-3,ADHX,FALDH,FDH,GSH-FDH,GSNOR,HEL-S-60p
General protein information	Preferred Names alcohol dehydrogenase 5 (class III), chi polypeptide Names alcohol dehydrogenase class-3 S-(hydroxymethyl)glutathione dehydrogenase S-nitrosoglutathione reductase alcohol dehydrogenase (class III), chi polypeptide alcohol dehydrogenase class chi chain alcohol dehydrogenase class-III epididymis secretory sperm binding protein Li 60p formaldehyde dehydrogenase glutathione-dependent formaldehyde dehydrogenase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q23
Summary	This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008].
Disorder MIM:	103710

mRNA and Protein(s)

mRNA	Protein	Name
NM_000671.4	NP_000662.3	alcohol dehydrogenase class-3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ADH5	NP_001029421.1
Eremothecium gossypii	AGOS_ACL148C	NP_983256.1
Schizosaccharomyces pombe (fission yeast)	SPCC13B11.04c	NP_588247.1
Xenopus tropicalis (tropical clawed frog)	adh5	NP_001011502.1
Canis lupus familiaris (dog)	ADH5	NP_001239084.1
Saccharomyces cerevisiae (baker's yeast)	SFA1	NP_010113.1
Kluyveromyces lactis	KLLA0D12342g	XP_453612.1
Oryza sativa (rice)	Os02g0815500	NP_001048503.1
Homo sapiens (human)	ADH5	NP_000662.3
Pan troglodytes (chimpanzee)	ADH5	XP_001166206.1
Mus musculus (house mouse)	Adh5	NP_031436.2
Rattus norvegicus (Norway rat)	Adh5	NP_001119592.1
Gallus gallus (chicken)	ADH5	NP_001026323.1
Caenorhabditis elegans (roundworm)	H24K24.3	NP_001024016.1
Caenorhabditis elegans (roundworm)	Y50D4C.2	NP_503357.1
Magnaporthe oryzae (rice blast fungus)	MGG_06011	XP_003711858.1
	LOC697703	XP_001088376.1
Danio rerio (zebrafish)	adh5	NP_571924.2
Drosophila melanogaster (fruit fly)	Fdh	NP_524310.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010499	XP_314472.4
Neurospora crassa	NCU06652	XP_960697.1
Arabidopsis thaliana (thale cress)	HOT5	NP_199207.1

Gene Ontology
Bibliography

Related articles in PubMed

Qian Q, Zhang Z, Orwig A, Chen S, Ding WX, Xu Y, Kunz RC, Lind NRL, Stamler JS, Yang L
Diabetes67(2)193-207(2018 Feb)

Genetic variant repressing ADH1A expression confers susceptibility to esophageal squamous-cell carcinoma.
Cui Q, Peng L, Wei L, Chang J, Tan W, Luo Y, Huang X, Zhao Y, Li J, Chu J, Shao M, Zhang C, Li C, Tan W, Lin D, Wu C
Cancer letters(2017 Dec)

Chronicles of a reductase: Biochemistry, genetics and physio-pathological role of GSNOR.
Rizza S, Filomeni G
Free radical biology & medicine11019-30(2017 Sep)

Posttranscriptional regulation of human ADH5/FDH and Myf6 gene expression by upstream AUG codons.
Kwon HS, Lee DK, Lee JJ, Edenberg HJ, Ahn YH, Hur MW
Archives of biochemistry and biophysics386(2)163-71(2001 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It was concluded that in HepG2 cells, ADH5 is a source of formate for de novo purine biosynthesis, especially during folate deficiency when folate-dependent formate production is limited.
Title: Alcohol Dehydrogenase 5 Is a Source of Formate for De Novo Purine Biosynthesis in HepG2 Cells.

GSNOR expression has different effect on neuronal viability in dependence on the stimulus applied, and plays opposite roles in SH-SY5Y models of Parkinson's disease and amyotrophic lateral sclerosis
Title: S-Nitrosoglutathione Reductase Plays Opposite Roles in SH-SY5Y Models of Parkinson's Disease and Amyotrophic Lateral Sclerosis.

ADH5 counteracts neuronal differentiation of neural stem cells and this effect can be reversed by pharmacological inhibition of ADH5.
Title: A novel suppressive effect of alcohol dehydrogenase 5 in neuronal differentiation.

common ADH variants conferred risk for both schizophrenia in African-Americans and autism in European-Americans.
Title: Association between common alcohol dehydrogenase gene (ADH) variants and schizophrenia and autism.

study compared individuals occupationally exposed to formaldehyde and controls to effects of XRCC3 Thr241Met, ADH5 Val309Ile and Asp353Glu polymorphisms; ADH5 polymorphisms did not show significant association with genotoxicity biomarkers
Title: The influence of genetic polymorphisms in XRCC3 and ADH5 genes on the frequency of genotoxicity biomarkers in workers exposed to formaldehyde.

The following ADH5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADH5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu24218	NM_000671.4 Latest version!	Homo sapiens alcohol dehydrogenase 5 (class III), chi polypeptide (ADH5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADH5 ( NM_000671.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000662.3 Homo sapiens alcohol dehydrogenase 5 (class III), chi polypeptide (ADH5), mRNA.

Price & Availability↑

CloneID	OHu24218
Clone ID Related Accession (Same CDS sequence)	NM_000671.4
Accession Version	NM_000671.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	alcohol dehydrogenase class-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA737788.1, M30471.1 and AC019131.7. On Dec 21, 2013 this sequence version replaced NM_000671.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M30471.1, SRR3476690.213168.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript regulatory uORF :: PMID: 11368338 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGAACG AGGTTATCAA GTGCAAGGCT GCAGTTGCTT GGGAGGCTGG AAAGCCTCTC 
TCCATAGAGG AGATAGAGGT GGCACCCCCA AAGGCTCATG AAGTTCGAAT CAAGATCATT 
GCCACTGCGG TTTGCCACAC CGATGCCTAT ACCCTGAGTG GAGCTGATCC TGAGGGTTGT 
TTTCCAGTGA TCTTGGGACA TGAAGGTGCT GGAATTGTGG AAAGTGTTGG TGAGGGAGTT 
ACTAAGCTGA AGGCGGGTGA CACTGTCATC CCACTTTACA TCCCACAGTG TGGAGAATGC 
AAATTTTGTC TAAATCCTAA AACTAACCTT TGCCAGAAGA TAAGAGTCAC TCAAGGGAAA 
GGATTAATGC CAGATGGTAC CAGCAGATTT ACTTGCAAAG GAAAGACAAT TTTGCATTAC 
ATGGGAACCA GCACATTTTC TGAATACACA GTTGTGGCTG ATATCTCTGT TGCTAAAATA 
GATCCTTTAG CACCTTTGGA TAAAGTCTGC CTTCTAGGTT GTGGCATTTC AACCGGTTAT 
GGTGCTGCTG TGAACACTGC CAAGTTGGAG CCTGGCTCTG TTTGTGCCGT CTTTGGTCTG 
GGAGGAGTCG GATTGGCAGT TATCATGGGC TGTAAAGTGG CTGGTGCTTC CCGGATCATT 
GGTGTGGACA TCAATAAAGA TAAATTTGCA AGGGCCAAAG AGTTTGGAGC CACTGAATGT 
ATTAACCCTC AGGATTTTAG TAAACCCATC CAGGAAGTGC TCATTGAGAT GACCGATGGA 
GGAGTGGACT ATTCCTTTGA ATGTATTGGT AATGTGAAGG TCATGAGAGC AGCACTTGAG 
GCATGTCACA AGGGCTGGGG CGTCAGCGTC GTGGTTGGAG TAGCTGCTTC AGGTGAAGAA 
ATTGCCACTC GTCCATTCCA GCTGGTAACA GGTCGCACAT GGAAAGGCAC TGCCTTTGGA 
GGATGGAAGA GTGTAGAAAG TGTCCCAAAG TTGGTGTCTG AATATATGTC CAAAAAGATA 
AAAGTTGATG AATTTGTGAC TCACAATCTG TCTTTTGATG AAATCAACAA AGCCTTTGAA 
CTGATGCATT CTGGAAAGAG CATTCGAACT GTTGTAAAGA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000662.3
CDS	89..1213
Translation	MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI

Target ORF information:

RefSeq Version	NM_000671.4
Organism	Homo sapiens(human)
Definition	Homo sapiens alcohol dehydrogenase 5 (class III), chi polypeptide (ADH5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000671.4

ORF Insert Sequence:

ATGGCGAACG AGGTTATCAA GTGCAAGGCT GCAGTTGCTT GGGAGGCTGG AAAGCCTCTC 
TCCATAGAGG AGATAGAGGT GGCACCCCCA AAGGCTCATG AAGTTCGAAT CAAGATCATT 
GCCACTGCGG TTTGCCACAC CGATGCCTAT ACCCTGAGTG GAGCTGATCC TGAGGGTTGT 
TTTCCAGTGA TCTTGGGACA TGAAGGTGCT GGAATTGTGG AAAGTGTTGG TGAGGGAGTT 
ACTAAGCTGA AGGCGGGTGA CACTGTCATC CCACTTTACA TCCCACAGTG TGGAGAATGC 
AAATTTTGTC TAAATCCTAA AACTAACCTT TGCCAGAAGA TAAGAGTCAC TCAAGGGAAA 
GGATTAATGC CAGATGGTAC CAGCAGATTT ACTTGCAAAG GAAAGACAAT TTTGCATTAC 
ATGGGAACCA GCACATTTTC TGAATACACA GTTGTGGCTG ATATCTCTGT TGCTAAAATA 
GATCCTTTAG CACCTTTGGA TAAAGTCTGC CTTCTAGGTT GTGGCATTTC AACCGGTTAT 
GGTGCTGCTG TGAACACTGC CAAGTTGGAG CCTGGCTCTG TTTGTGCCGT CTTTGGTCTG 
GGAGGAGTCG GATTGGCAGT TATCATGGGC TGTAAAGTGG CTGGTGCTTC CCGGATCATT 
GGTGTGGACA TCAATAAAGA TAAATTTGCA AGGGCCAAAG AGTTTGGAGC CACTGAATGT 
ATTAACCCTC AGGATTTTAG TAAACCCATC CAGGAAGTGC TCATTGAGAT GACCGATGGA 
GGAGTGGACT ATTCCTTTGA ATGTATTGGT AATGTGAAGG TCATGAGAGC AGCACTTGAG 
GCATGTCACA AGGGCTGGGG CGTCAGCGTC GTGGTTGGAG TAGCTGCTTC AGGTGAAGAA 
ATTGCCACTC GTCCATTCCA GCTGGTAACA GGTCGCACAT GGAAAGGCAC TGCCTTTGGA 
GGATGGAAGA GTGTAGAAAG TGTCCCAAAG TTGGTGTCTG AATATATGTC CAAAAAGATA 
AAAGTTGATG AATTTGTGAC TCACAATCTG TCTTTTGATG AAATCAACAA AGCCTTTGAA 
CTGATGCATT CTGGAAAGAG CATTCGAACT GTTGTAAAGA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diabetes67(2)193-207(2018 Feb)
Qian Q,Zhang Z,Orwig A,Chen S,Ding WX,Xu Y,Kunz RC,Lind NRL,Stamler JS,Yang L

Genetic variant repressing ADH1A expression confers susceptibility to esophageal squamous-cell carcinoma.
Cancer letters(2017 Dec)
Cui Q,Peng L,Wei L,Chang J,Tan W,Luo Y,Huang X,Zhao Y,Li J,Chu J,Shao M,Zhang C,Li C,Tan W,Lin D,Wu C

Chronicles of a reductase: Biochemistry, genetics and physio-pathological role of GSNOR.
Free radical biology & medicine11019-30(2017 Sep)
Rizza S,Filomeni G

Posttranscriptional regulation of human ADH5/FDH and Myf6 gene expression by upstream AUG codons.
Archives of biochemistry and biophysics386(2)163-71(2001 Feb)
Kwon HS,Lee DK,Lee JJ,Edenberg HJ,Ahn YH,Hur MW

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ADH5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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