CNTF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CNTF
Entrez Gene ID	1270
Full Name	ciliary neurotrophic factor
Synonyms	HCNTF
General protein information	Preferred Names ciliary neurotrophic factor Names ciliary neurotrophic factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q12.1
Summary	The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010].
Disorder MIM:	118945

mRNA and Protein(s)

mRNA	Protein	Name
NM_000614.3	NP_000605.1	ciliary neurotrophic factor
NM_000614.4	NP_000605.1	ciliary neurotrophic factor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CNTF	XP_003318038.1
	CNTF	XP_001093229.1
Canis lupus familiaris (dog)	CNTF	XP_005631246.1
Bos taurus (cattle)	CNTF	XP_003587079.1
Homo sapiens (human)	CNTF	NP_000605.1
Mus musculus (house mouse)	Cntf	NP_740756.1
Rattus norvegicus (Norway rat)	Cntf	NP_037298.1
Gallus gallus (chicken)	CNTF	NP_990823.1

Gene Ontology
Bibliography

Related articles in PubMed

Hypomethylation of CNTFR? is associated with proliferation and poor prognosis in lower grade gliomas.
Fan K, Wang X, Zhang J, Ramos RI, Zhang H, Li C, Ye D, Kang J, Marzese DM, Hoon DSB, Hua W
Scientific reports7(1)7079(2017 Aug)

High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration.
Wang Q, Liu Y, Zhang C, Guo F, Feng C, Li X, Shi H, Su Z
Protein expression and purification133152-159(2017 May)

Cytokines of the LIF/CNTF family and metabolism.
Pasquin S, Sharma M, Gauchat JF
Cytokine82122-4(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration.
Title: High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration.

Cytokines of the LIF/CNTF family and metabolism
Title: Cytokines of the LIF/CNTF family and metabolism.

CNTFR-specific mutants of CNTF have been developed that bind to the CNTFRalpha-LIFRbeta-gp130 receptor.
Title: Ciliary neurotrophic factor (CNTF): New facets of an old molecule for treating neurodegenerative and metabolic syndrome pathologies.

Human CNTF expression through lentiviral gene transfer in the rat striatum significantly decreased the levels of neuronal metabolites (N-acetyl-aspartate, N-acetyl-aspartyl-glutamate, and glutamate).
Title: The neuroprotective agent CNTF decreases neuronal metabolites in the rat striatum: an in vivo multimodal magnetic resonance imaging study.

the biological effects of CNTF on retinoic acid (RA)-predifferentiated SH-SY5Y neuroblastoma cells and the underlying molecular mechanism of this effect were investigated for the first time
Title: Neuroprotective properties of ciliary neurotrophic factor on retinoic acid (RA)-predifferentiated SH-SY5Y neuroblastoma cells.

The following CNTF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNTF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26060	NM_000614.4 Latest version!	Homo sapiens ciliary neurotrophic factor (CNTF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
View Old Accession Versions >>
OHu26060	NM_000614.3	Homo sapiens ciliary neurotrophic factor (CNTF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNTF ( NM_000614.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000605.1 Homo sapiens ciliary neurotrophic factor (CNTF), mRNA.

Price & Availability↑

CloneID	OHu26060
Clone ID Related Accession (Same CDS sequence)	NM_000614.3 , NM_000614.4
Accession Version	NM_000614.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	ciliary neurotrophic factor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB241497.1, BC068030.1 and DB341875.1. This sequence is a reference standard in the RefSeqGene project. On Oct 17, 2008 this sequence version replaced NM_000614.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC068030.1, SRR1660807.25441.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTCA CAGAGCATTC ACCGCTGACC CCTCACCGTC GGGACCTCTG TAGCCGCTCT 
ATCTGGCTAG CAAGGAAGAT TCGTTCAGAC CTGACTGCTC TTACGGAATC CTATGTGAAG 
CATCAGGGCC TGAACAAGAA CATCAACCTG GACTCTGCGG ATGGGATGCC AGTGGCAAGC 
ACTGATCAGT GGAGTGAGCT GACCGAGGCA GAGCGACTCC AAGAGAACCT TCAAGCTTAT 
CGTACCTTCC ATGTTTTGTT GGCCAGGCTC TTAGAAGACC AGCAGGTGCA TTTTACCCCA 
ACCGAAGGTG ACTTCCATCA AGCTATACAT ACCCTTCTTC TCCAAGTCGC TGCCTTTGCA 
TACCAGATAG AGGAGTTAAT GATACTCCTG GAATACAAGA TCCCCCGCAA TGAGGCTGAT 
GGGATGCCTA TTAATGTTGG AGATGGTGGT CTCTTTGAGA AGAAGCTGTG GGGCCTAAAG 
GTGCTGCAGG AGCTTTCACA GTGGACAGTA AGGTCCATCC ATGACCTTCG TTTCATTTCT 
TCTCATCAGA CTGGGATCCC AGCACGTGGG AGCCATTATA TTGCTAACAA CAAGAAAATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000605.1
CDS	81..683
Translation	MAFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFAYQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFISSHQTGIPARGSHYIANNKKM

Target ORF information:

RefSeq Version	NM_000614.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ciliary neurotrophic factor (CNTF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000614.3

ORF Insert Sequence:

ATGGCTTTCA CAGAGCATTC ACCGCTGACC CCTCACCGTC GGGACCTCTG TAGCCGCTCT 
ATCTGGCTAG CAAGGAAGAT TCGTTCAGAC CTGACTGCTC TTACGGAATC CTATGTGAAG 
CATCAGGGCC TGAACAAGAA CATCAACCTG GACTCTGCGG ATGGGATGCC AGTGGCAAGC 
ACTGATCAGT GGAGTGAGCT GACCGAGGCA GAGCGACTCC AAGAGAACCT TCAAGCTTAT 
CGTACCTTCC ATGTTTTGTT GGCCAGGCTC TTAGAAGACC AGCAGGTGCA TTTTACCCCA 
ACCGAAGGTG ACTTCCATCA AGCTATACAT ACCCTTCTTC TCCAAGTCGC TGCCTTTGCA 
TACCAGATAG AGGAGTTAAT GATACTCCTG GAATACAAGA TCCCCCGCAA TGAGGCTGAT 
GGGATGCCTA TTAATGTTGG AGATGGTGGT CTCTTTGAGA AGAAGCTGTG GGGCCTAAAG 
GTGCTGCAGG AGCTTTCACA GTGGACAGTA AGGTCCATCC ATGACCTTCG TTTCATTTCT 
TCTCATCAGA CTGGGATCCC AGCACGTGGG AGCCATTATA TTGCTAACAA CAAGAAAATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNTF ( NM_000614.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000605.1 Homo sapiens ciliary neurotrophic factor (CNTF), mRNA.

Price & Availability↑

CloneID	OHu26060
Clone ID Related Accession (Same CDS sequence)	NM_000614.3 , NM_000614.4
Accession Version	NM_000614.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	ciliary neurotrophic factor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001350.4, DB241497.1, BC068030.1 and DB341875.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000614.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC068030.1, SRR1660807.25441.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361987.6/ ENSP00000355370.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTTTCA CAGAGCATTC ACCGCTGACC CCTCACCGTC GGGACCTCTG TAGCCGCTCT 
ATCTGGCTAG CAAGGAAGAT TCGTTCAGAC CTGACTGCTC TTACGGAATC CTATGTGAAG 
CATCAGGGCC TGAACAAGAA CATCAACCTG GACTCTGCGG ATGGGATGCC AGTGGCAAGC 
ACTGATCAGT GGAGTGAGCT GACCGAGGCA GAGCGACTCC AAGAGAACCT TCAAGCTTAT 
CGTACCTTCC ATGTTTTGTT GGCCAGGCTC TTAGAAGACC AGCAGGTGCA TTTTACCCCA 
ACCGAAGGTG ACTTCCATCA AGCTATACAT ACCCTTCTTC TCCAAGTCGC TGCCTTTGCA 
TACCAGATAG AGGAGTTAAT GATACTCCTG GAATACAAGA TCCCCCGCAA TGAGGCTGAT 
GGGATGCCTA TTAATGTTGG AGATGGTGGT CTCTTTGAGA AGAAGCTGTG GGGCCTAAAG 
GTGCTGCAGG AGCTTTCACA GTGGACAGTA AGGTCCATCC ATGACCTTCG TTTCATTTCT 
TCTCATCAGA CTGGGATCCC AGCACGTGGG AGCCATTATA TTGCTAACAA CAAGAAAATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000605.1
CDS	89..691
Translation	MAFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPTEGDFHQAIHTLLLQVAAFAYQIEELMILLEYKIPRNEADGMPINVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRFISSHQTGIPARGSHYIANNKKM

Target ORF information:

RefSeq Version	NM_000614.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ciliary neurotrophic factor (CNTF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000614.4

ORF Insert Sequence:

ATGGCTTTCA CAGAGCATTC ACCGCTGACC CCTCACCGTC GGGACCTCTG TAGCCGCTCT 
ATCTGGCTAG CAAGGAAGAT TCGTTCAGAC CTGACTGCTC TTACGGAATC CTATGTGAAG 
CATCAGGGCC TGAACAAGAA CATCAACCTG GACTCTGCGG ATGGGATGCC AGTGGCAAGC 
ACTGATCAGT GGAGTGAGCT GACCGAGGCA GAGCGACTCC AAGAGAACCT TCAAGCTTAT 
CGTACCTTCC ATGTTTTGTT GGCCAGGCTC TTAGAAGACC AGCAGGTGCA TTTTACCCCA 
ACCGAAGGTG ACTTCCATCA AGCTATACAT ACCCTTCTTC TCCAAGTCGC TGCCTTTGCA 
TACCAGATAG AGGAGTTAAT GATACTCCTG GAATACAAGA TCCCCCGCAA TGAGGCTGAT 
GGGATGCCTA TTAATGTTGG AGATGGTGGT CTCTTTGAGA AGAAGCTGTG GGGCCTAAAG 
GTGCTGCAGG AGCTTTCACA GTGGACAGTA AGGTCCATCC ATGACCTTCG TTTCATTTCT 
TCTCATCAGA CTGGGATCCC AGCACGTGGG AGCCATTATA TTGCTAACAA CAAGAAAATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hypomethylation of CNTFR? is associated with proliferation and poor prognosis in lower grade gliomas.
Scientific reports7(1)7079(2017 Aug)
Fan K,Wang X,Zhang J,Ramos RI,Zhang H,Li C,Ye D,Kang J,Marzese DM,Hoon DSB,Hua W

High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration.
Protein expression and purification133152-159(2017 May)
Wang Q,Liu Y,Zhang C,Guo F,Feng C,Li X,Shi H,Su Z

Cytokines of the LIF/CNTF family and metabolism.
Cytokine82122-4(2016 Jun)
Pasquin S,Sharma M,Gauchat JF

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CNTF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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