HIST2H3C cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIST2H3C
Entrez Gene ID	126961
Full Name	histone cluster 2 H3 family member c
Synonyms	H3,H3.2,H3/M,H3F2,H3FM,H3FN
General protein information	Preferred Names histone cluster 2 H3 family member c Names histone H3.2 H3 histone family, member M H3 histone, family 2 histone 2, H3c histone H3/m histone cluster 2, H3c
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q21.2
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Aug 2015].
Disorder MIM:	142780

mRNA and Protein(s)

mRNA	Protein	Name
NM_021059.3	NP_066403.2	histone H3.2
NM_021059.2	NP_066403.2	histone H3.2

Pathways from BioSystems

Homologies

	LOC710748	XP_001109042.1
Bos taurus (cattle)	LOC781224	XP_005208273.1
Drosophila melanogaster (fruit fly)	His3:CG33824	NP_001027318.1
Xenopus tropicalis (tropical clawed frog)	LOC100486720	XP_002944964.1
Homo sapiens (human)	HIST2H3C	NP_066403.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012709	XP_307081.3
Rattus norvegicus (Norway rat)	H3f3c	XP_003750396.1
Pan troglodytes (chimpanzee)	LOC469476	XP_524859.2
Canis lupus familiaris (dog)	LOC100856221	XP_003638893.1
Mus musculus (house mouse)	H3f3c	XP_894986.1

Gene Ontology
Bibliography

Related articles in PubMed

Global analysis of H3K27me3 as an epigenetic marker in prostate cancer progression.
Ngollo M, Lebert A, Daures M, Judes G, Rifai K, Dubois L, Kemeny JL, Penault-Llorca F, Bignon YJ, Guy L, Bernard-Gallon D
BMC cancer17(1)261(2017 04)

Molecular basis for the role of oncogenic histone mutations in modulating H3K36 methylation.
Zhang Y, Shan CM, Wang J, Bao K, Tong L, Jia S
Scientific reports743906(2017 Mar)

Functional characterization of a human histone gene cluster duplication.
Braastad CD, Hovhannisyan H, van Wijnen AJ, Stein JL, Stein GS
Gene342(1)35-40(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings point to epigenetic mark H3K27me3 as an important event in prostate carcinogenesis and progression.
Title: Global analysis of H3K27me3 as an epigenetic marker in prostate cancer progression.

This study presented the H3 K27M mutations in posterior fossa ependymomas.
Title: Evidence of H3 K27M mutations in posterior fossa ependymomas.

Results identified histone H3K27me3 as elevated in aggressive melanoma cells relative to less aggressive melanoma cells in cell culture and in patient tissues.
Title: Quantitative Histone Mass Spectrometry Identifies Elevated Histone H3 Lysine 27 (Lys27) Trimethylation in Melanoma.

Study identified phosphorylation of histone H3 at serine 10 (H3S10ph) as a histone post-translational modifications with most consistent and significant difference between gastric cancer (GC) tumor and negative resection margin. H3S10 phosphorylation in GC is mediated by MSK1 through the p38- MAPK pathway.
Title: p38-MAPK/MSK1-mediated overexpression of histone H3 serine 10 phosphorylation defines distance-dependent prognostic value of negative resection margin in gastric cancer.

Study shows proteolytic processing of H2B and H3 in hepatocytes where four different clipping sites were localized in H3 and one in H2B. Also, clipped H3 carry distinct co-existing post-translational modifications but different from those in intact H3.
Title: Top-down and Middle-down Protein Analysis Reveals that Intact and Clipped Human Histones Differ in Post-translational Modification Patterns.

The following HIST2H3C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIST2H3C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13909	NM_021059.3 Latest version!	Homo sapiens H3 clustered histone 14 (H3C14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu13909	NM_021059.2	Homo sapiens histone cluster 2 H3 family member c (HIST2H3C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIST2H3C ( NM_021059.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066403.2 Homo sapiens H3 clustered histone 14 (H3C14), mRNA.

Price & Availability↑

CloneID	OHu13909
Clone ID Related Accession (Same CDS sequence)	NM_021059.3 , NM_021059.2
Accession Version	NM_021059.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	histone H3.2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC239868.2. On Dec 3, 2019 this sequence version replaced NM_021059.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC015544.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCGTA CTAAGCAGAC TGCTCGCAAG TCGACCGGCG GCAAGGCCCC GAGGAAGCAG 
CTGGCCACCA AGGCGGCCCG CAAGAGCGCG CCGGCCACGG GCGGGGTGAA GAAGCCGCAC 
CGCTACCGGC CCGGCACCGT AGCCCTGCGG GAGATCCGGC GCTACCAGAA GTCCACGGAG 
CTGCTGATCC GCAAGCTGCC CTTCCAGCGG CTGGTACGCG AGATCGCGCA GGACTTTAAG 
ACGGACCTGC GCTTCCAGAG CTCGGCCGTG ATGGCGCTGC AGGAGGCCAG CGAGGCCTAC 
CTGGTGGGGC TGTTCGAAGA CACGAACCTG TGCGCCATCC ACGCCAAGCG CGTGACCATT 
ATGCCCAAGG ACATCCAGCT GGCCCGCCGC ATCCGTGGAG AGCGGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066403.2
CDS	52..462
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	NM_021059.3
Organism	Homo sapiens(human)
Definition	Homo sapiens H3 clustered histone 14 (H3C14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021059.3

ORF Insert Sequence:

ATGGCCCGTA CTAAGCAGAC TGCTCGCAAG TCGACCGGCG GCAAGGCCCC GAGGAAGCAG 
CTGGCCACCA AGGCGGCCCG CAAGAGCGCG CCGGCCACGG GCGGGGTGAA GAAGCCGCAC 
CGCTACCGGC CCGGCACCGT AGCCCTGCGG GAGATCCGGC GCTACCAGAA GTCCACGGAG 
CTGCTGATCC GCAAGCTGCC CTTCCAGCGG CTGGTACGCG AGATCGCGCA GGACTTTAAG 
ACGGACCTGC GCTTCCAGAG CTCGGCCGTG ATGGCGCTGC AGGAGGCCAG CGAGGCCTAC 
CTGGTGGGGC TGTTCGAAGA CACGAACCTG TGCGCCATCC ACGCCAAGCG CGTGACCATT 
ATGCCCAAGG ACATCCAGCT GGCCCGCCGC ATCCGTGGAG AGCGGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIST2H3C ( NM_021059.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_066403.2 Homo sapiens histone cluster 2 H3 family member c (HIST2H3C), mRNA.

Price & Availability↑

CloneID	OHu13909
Clone ID Related Accession (Same CDS sequence)	NM_021059.3 , NM_021059.2
Accession Version	NM_021059.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	histone H3.2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF531307.1. On or before Sep 20, 2006 this sequence version replaced XM_001124743.1, NM_152368.1, NM_021059.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCGTA CTAAGCAGAC TGCTCGCAAG TCGACCGGCG GCAAGGCCCC GAGGAAGCAG 
CTGGCCACCA AGGCGGCCCG CAAGAGCGCG CCGGCCACGG GCGGGGTGAA GAAGCCGCAC 
CGCTACCGGC CCGGCACCGT AGCCCTGCGG GAGATCCGGC GCTACCAGAA GTCCACGGAG 
CTGCTGATCC GCAAGCTGCC CTTCCAGCGG CTGGTACGCG AGATCGCGCA GGACTTTAAG 
ACGGACCTGC GCTTCCAGAG CTCGGCCGTG ATGGCGCTGC AGGAGGCCAG CGAGGCCTAC 
CTGGTGGGGC TGTTCGAAGA CACGAACCTG TGCGCCATCC ACGCCAAGCG CGTGACCATT 
ATGCCCAAGG ACATCCAGCT GGCCCGCCGC ATCCGTGGAG AGCGGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066403.2
CDS	37..447
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	NM_021059.2
Organism	Homo sapiens(human)
Definition	Homo sapiens histone cluster 2 H3 family member c (HIST2H3C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021059.2

ORF Insert Sequence:

ATGGCCCGTA CTAAGCAGAC TGCTCGCAAG TCGACCGGCG GCAAGGCCCC GAGGAAGCAG 
CTGGCCACCA AGGCGGCCCG CAAGAGCGCG CCGGCCACGG GCGGGGTGAA GAAGCCGCAC 
CGCTACCGGC CCGGCACCGT AGCCCTGCGG GAGATCCGGC GCTACCAGAA GTCCACGGAG 
CTGCTGATCC GCAAGCTGCC CTTCCAGCGG CTGGTACGCG AGATCGCGCA GGACTTTAAG 
ACGGACCTGC GCTTCCAGAG CTCGGCCGTG ATGGCGCTGC AGGAGGCCAG CGAGGCCTAC 
CTGGTGGGGC TGTTCGAAGA CACGAACCTG TGCGCCATCC ACGCCAAGCG CGTGACCATT 
ATGCCCAAGG ACATCCAGCT GGCCCGCCGC ATCCGTGGAG AGCGGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Global analysis of H3K27me3 as an epigenetic marker in prostate cancer progression.
BMC cancer17(1)261(2017 04)
Ngollo M,Lebert A,Daures M,Judes G,Rifai K,Dubois L,Kemeny JL,Penault-Llorca F,Bignon YJ,Guy L,Bernard-Gallon D

Molecular basis for the role of oncogenic histone mutations in modulating H3K36 methylation.
Scientific reports743906(2017 Mar)
Zhang Y,Shan CM,Wang J,Bao K,Tong L,Jia S

Functional characterization of a human histone gene cluster duplication.
Gene342(1)35-40(2004 Nov)
Braastad CD,Hovhannisyan H,van Wijnen AJ,Stein JL,Stein GS

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

HIST2H3C cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報