ANKLE1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKLE1
Entrez Gene ID	126549
Full Name	ankyrin repeat and LEM domain containing 1
Synonyms	ANKRD41,LEM3,LEMD6
General protein information	Preferred Names ankyrin repeat and LEM domain containing 1 Names ankyrin repeat and LEM domain-containing protein 1 LEM domain containing 6 LEM-domain containing 3 ankyrin repeat domain-containing protein 41
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001278444.1	NP_001265373.1	ankyrin repeat and LEM domain-containing protein 1 isoform 3
NM_001278445.2	NP_001265374.2	ankyrin repeat and LEM domain-containing protein 1 isoform 4
NM_152363.6	NP_689576.6	ankyrin repeat and LEM domain-containing protein 1 isoform 1
NM_001278445.1	NP_001265374.1	ankyrin repeat and LEM domain-containing protein 1 isoform 4
NM_001278443.2	NP_001265372.2	ankyrin repeat and LEM domain-containing protein 1 isoform 2
NM_001278444.2	NP_001265373.2	ankyrin repeat and LEM domain-containing protein 1 isoform 3
NM_152363.5	NP_689576.5	ankyrin repeat and LEM domain-containing protein 1 isoform 1
NM_001278443.1	NP_001265372.1	ankyrin repeat and LEM domain-containing protein 1 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ANKLE1	NP_689576.5
Bos taurus (cattle)	ANKLE1	XP_005208615.1
	ANKLE1	XP_001114324.2
Rattus norvegicus (Norway rat)	Ankle1	XP_006253022.1
Pan troglodytes (chimpanzee)	ANKLE1	XP_001173375.2
Canis lupus familiaris (dog)	ANKLE1	XP_852664.2
Mus musculus (house mouse)	Ankle1	NP_766344.2

Gene Ontology
Bibliography

Related articles in PubMed

Identification of breast cancer associated variants that modulate transcription factor binding.
Liu Y, Walavalkar NM, Dozmorov MG, Rich SS, Civelek M, Guertin MJ
PLoS genetics13(9)e1006761(2017 Sep)

Cis-eQTL-based trans-ethnic meta-analysis reveals novel genes associated with breast cancer risk.
Hoffman JD, Graff RE, Emami NC, Tai CG, Passarelli MN, Hu D, Huntsman S, Hadley D, Leong L, Majumdar A, Zaitlen N, Ziv E, Witte JS
PLoS genetics13(3)e1006690(2017 Mar)

Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals.
Zlopasa L, Brachner A, Foisner R
BMC cell biology17(1)23(2016 06)

Genome-wide association study identifies 25 known breast cancer susceptibility loci as risk factors for triple-negative breast cancer.
Purrington KS, Slager S, Eccles D, Yannoukakos D, Fasching PA, Miron P, Carpenter J, Chang-Claude J, Martin NG, Montgomery GW, Kristensen V, Anton-Culver H, Goodfellow P, Tapper WJ, Rafiq S, Gerty SM, Durcan L, Konstantopoulou I, Fostira F, Vratimos A, Apostolou P, Konstanta I, Kotoula V, Lakis S, Dimopoulos MA, Skarlos D, Pectasides D, Fountzilas G, Beckmann MW, Hein A, Ruebner M, Ekici AB, Hartmann A, Schulz-Wendtland R, Renner SP, Janni W, Rack B, Scholz C, Neugebauer J, Andergassen U, Lux MP, Haeberle L, Clarke C, Pathmanathan N, Rudolph A, Flesch-Janys D, Nickels S, Olson JE, Ingle JN, Olswold C, Slettedahl S, Eckel-Passow JE, Anderson SK, Visscher DW, Cafourek VL, Sicotte H, Prodduturi N, Weiderpass E, Bernstein L, Ziogas A, Ivanovich J, Giles GG, Baglietto L, Southey M, Kosma VM, Fischer HP, , Reed MW, Cross SS, Deming-Halverson S, Shrubsole M, Cai Q, Shu XO, Daly M, Weaver J, Ross E, Klemp J, Sharma P, Torres D, R?diger T, W?lfing H, Ulmer HU, F?rsti A, Khoury T, Kumar S, Pilarski R, Shapiro CL, Greco D, Heikkil? P, Aittom?ki K, Blomqvist C, Irwanto A, Liu J, Pankratz VS, Wang X, Severi G, Mannermaa A, Easton D, Hall P, Brauch H, Cox A, Zheng W, Godwin AK, Hamann U, Ambrosone C, Toland AE, Nevanlinna H, Vachon CM, Couch FJ
Carcinogenesis35(5)1012-9(2014 May)

A meta-analysis of genome-wide association studies of breast cancer identifies two novel susceptibility loci at 6q14 and 20q11.
Siddiq A, Couch FJ, Chen GK, Lindstr?m S, Eccles D, Millikan RC, Michailidou K, Stram DO, Beckmann L, Rhie SK, Ambrosone CB, Aittom?ki K, Amiano P, Apicella C, , Baglietto L, Bandera EV, Beckmann MW, Berg CD, Bernstein L, Blomqvist C, Brauch H, Brinton L, Bui QM, Buring JE, Buys SS, Campa D, Carpenter JE, Chasman DI, Chang-Claude J, Chen C, Clavel-Chapelon F, Cox A, Cross SS, Czene K, Deming SL, Diasio RB, Diver WR, Dunning AM, Durcan L, Ekici AB, Fasching PA, , Feigelson HS, Fejerman L, Figueroa JD, Fletcher O, Flesch-Janys D, Gaudet MM, , Gerty SM, Rodriguez-Gil JL, Giles GG, van Gils CH, Godwin AK, Graham N, Greco D, Hall P, Hankinson SE, Hartmann A, Hein R, Heinz J, Hoover RN, Hopper JL, Hu JJ, Huntsman S, Ingles SA, Irwanto A, Isaacs C, Jacobs KB, John EM, Justenhoven C, Kaaks R, Kolonel LN, Coetzee GA, Lathrop M, Le Marchand L, Lee AM, Lee IM, Lesnick T, Lichtner P, Liu J, Lund E, Makalic E, Martin NG, McLean CA, Meijers-Heijboer H, Meindl A, Miron P, Monroe KR, Montgomery GW, M?ller-Myhsok B, Nickels S, Nyante SJ, Olswold C, Overvad K, Palli D, Park DJ, Palmer JR, Pathak H, Peto J, Pharoah P, Rahman N, Rivadeneira F, Schmidt DF, Schmutzler RK, Slager S, Southey MC, Stevens KN, Sinn HP, Press MF, Ross E, Riboli E, Ridker PM, Schumacher FR, Severi G, Dos Santos Silva I, Stone J, Sund M, Tapper WJ, Thun MJ, Travis RC, Turnbull C, Uitterlinden AG, Waisfisz Q, Wang X, Wang Z, Weaver J, Schulz-Wendtland R, Wilkens LR, Van Den Berg D, Zheng W, Ziegler RG, Ziv E, Nevanlinna H, Easton DF, Hunter DJ, Henderson BE, Chanock SJ, Garcia-Closas M, Kraft P, Haiman CA, Vachon CM
Human molecular genetics21(24)5373-84(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We used The Cancer Genome Atlas breast cancer patient data to identify ANKLE1 and ZNF404 as the target genes of candidate TF binding site SNPs in the 19p13.11 and 19q13.31 GWAS-identified loci. These SNPs are associated with the expression of ZNF404 and ANKLE1 in breast tissue.
Title: Identification of breast cancer associated variants that modulate transcription factor binding.

Expression of RCCD1 in whole blood was also suggestively associated with disease risk (p-value: 1.2x10-05), as were expression of ACAP1 (p-value: 1.9x10-05) and LRRC25 (p-value: 5.2x10-05). While genome-wide association studies (GWAS) have implicated RCCD1 and ANKLE1 in breast cancer risk, they have not identified the remaining three genes
Title: Cis-eQTL-based trans-ethnic meta-analysis reveals novel genes associated with breast cancer risk.

the domains mediating nuclear import and export of Ankle1, were identified.
Title: Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals.

Ankle1 is conserved in metazoans and contains a unique C-terminal GIY-YIG motif that confers endonuclease activity in vitro and in vivo
Title: The endonuclease Ankle1 requires its LEM and GIY-YIG motifs for DNA cleavage in vivo.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Common variants at 19p13 are associated with susceptibility to ovarian cancer.

The following ANKLE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKLE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12449	NM_152363.5 Latest version!	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$259.50-$363.30 $519.00
OHu00061	NM_001278445.1 Latest version!	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu11312	NM_001278443.1 Latest version!	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu107147	NM_152363.6 Latest version!	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu107149	NM_001278445.2 Latest version!	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu107152	NM_001278444.2 Latest version!	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu107156	NM_001278443.2 Latest version!	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu11133	NM_001278444.1 Latest version!	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKLE1 ( NM_152363.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_689576.5 Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12449
Clone ID Related Accession (Same CDS sequence)	NM_152363.5
Accession Version	NM_152363.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Homo sapiens(human)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK303628.1, AK096688.1 and AC010463.6. On Jun 6, 2013 this sequence version replaced NM_152363.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.66042.1, SRR1803614.280551.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004, SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGACCC CCGTGCGGCG CGGGGCAGCC TGGCTGGGAA CCCTGCTCGG CCCTGGAGAG 
GGGCCCCCCG AGCCGAGGGG AGGAGCGGCG CATGCCCACA AGCCCCCCGC GGGAAGTAGG 
AAATCGACCG ACCAGGCGGT GCGCTTCGGA CCCAGCCAGG GCATGTGCTC GGAGGCCCGC 
CTGGCTCGCA GGTTGCGGGA TGCGCTGCGG GAGGAGGAGC CGTGGGCAGT AGAGGAGCTG 
CTGCGCTGCG GCGCGGACCC TAATTTGGTG CTAGAGGACG GCGCAGCGGC TGTGCACTTG 
GCGGCCGGAG CCCGGCACCC GCGCGGCCTG CGTTGCCTCG GGGCCCTACT GCGCCAAGGC 
GGGGACCCCA ACGCTCGATC TGTCGAGGCA CTGACGCCGC TGCATGTGGC CGCCGCGTGG 
GGCTGCCGCC GCGGCCTGGA GCTGCTGCTG AGCCAAGGAG CGGACCCGGC GCTGCGCGAC 
CAGGACGGAC TCCGGCCGCT GGACCTGGCC CTGCAGCAGG GACACCTGGA GTGCGCGCGA 
GTCCTGCAGG ATCTCGACAC GCGGACCAGG ACCCGGACCC GGATCGGGGC AGAGACTCAG 
GAGCCCGAGC CTGCACCTGG CACCCCAGGC CTCTCTGGAC CTACCGATGA GACGCTGGAC 
TCCATAGCAC TCCAAAAGCA GCCATGCAGA GGTGACAACA GGGACATTGG CTTGGAGGCT 
GACCCAGGAC CCCCCAGCCT CCCTGTTCCC CTTGAAACTG TGGACAAACA TGGGAGCTCG 
GCGTCCCCTC CAGGGCACTG GGATTACAGC TCAGACGCCT CTTTCGTCAC AGCGGTTGAG 
GTCTCTGGAG CTGAGGACCC AGCCTCGGAC ACTCCCCCCT GGGCTGGGTC ATTGCCACCG 
ACCAGGCAGG GACTTCTGCA TGTTGTCCAT GCCAACCAGA GGGTACCTAG GTCTCAGGGC 
ACGGAGGCAG AACTGAATGC CCGTCTGCAG GCCCTGACTC TGACCCCACC AAATGCTGCT 
GGCTTCCAGT CCTCCCCTTC CTCCATGCCT CTCCTGGACA GGAGTCCAGC TCATAGCCCC 
CCACGGACAC CAACCCCTGG AGCTTCTGAC TGCCACTGCC TGTGGGAGCA CCAGACATCC 
ATTGATAGTG ACATGGCCAC GCTCTGGCTG ACAGAGGATG AGGCAAGCTC TACAGGTGGC 
AGGGAACCTG TCGGCCCTTG CCGGCACCTG CCAGTCTCCA CTGTGTCTGA CTTGGAGTTG 
CTGAAGGGAC TCCGAGCACT TGGTGAGAAT CCTCACCCCA TCACACCCTT CACCAGGCAG 
TTGTACCACC AGCAGCTGGA AGAAGCCCAG ATTGCTCCTG GCCCAGAGTT TTCAGGGCAC 
AGCCTAGAAC TGGCTGCAGC CCTGCGGACG GGCTGTATTC CAGATGTCCA GGCAGATGAA 
GACGCGCTGG CCCAGCAGTT TGAGCAGCCA GATCCTGCCA GGAGGTGGCG GGAGGGGGTC 
GTGAAGTCTA GCTTCACCTA TCTGCTGCTG GACCCCAGGG AGACTCAGGA CCTGCCAGCC 
CGAGCCTTCT CACTGACCCC AGCTGAGCGC CTTCAGACTT TCATCCGTGC CATCTTCTAC 
GTGGGCAAAG GGACGAGGGC CCGGCCATAT GTCCACCTCT GGGAGGCCCT TGGTCACCAT 
GGGCGGTCAA GAAAACAGCC CCACCAGGCC TGCCCCAAGG TGCGTCAGAT CTTGGACATC 
TGGGCCAGTG GTTGCGGTGT TGTGTCCCTA CATTGCTTCC AGCACGTGGT CGCTGTGGAG 
GCTTATACAC GGGAGGCGTG TATTGTGGAA GCCCTAGGGA TCCAGACGCT CACCAACCAG 
AAGCAAGGGC ACTGCTATGG AGTGGTGGCA GGCTGGCCAC CTGCTCGTCG CCGGCGCTTG 
GGGGTGCACC TGCTGCACCG TGCCCTCCTT GTCTTCCTGG CTGAAGGCGA GCGACAGCTT 
CATCCCCAGG ACATCCAGGC CCGGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689576.5
CDS	115..2124
Translation	MWTPVRRGAAWLGTLLGPGEGPPEPRGGAAHAHKPPAGSRKSTDQAVRFGPSQGMCSEARLARRLRDALREEEPWAVEELLRCGADPNLVLEDGAAAVHLAAGARHPRGLRCLGALLRQGGDPNARSVEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGLRPLDLALQQGHLECARVLQDLDTRTRTRTRIGAETQEPEPAPGTPGLSGPTDETLDSIALQKQPCRGDNRDIGLEADPGPPSLPVPLETVDKHGSSASPPGHWDYSSDASFVTAVEVSGAEDPASDTPPWAGSLPPTRQGLLHVVHANQRVPRSQGTEAELNARLQALTLTPPNAAGFQSSPSSMPLLDRSPAHSPPRTPTPGASDCHCLWEHQTSIDSDMATLWLTEDEASSTGGREPVGPCRHLPVSTVSDLELLKGLRALGENPHPITPFTRQLYHQQLEEAQIAPGPEFSGHSLELAAALRTGCIPDVQADEDALAQQFEQPDPARRWREGVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKGTRARPYVHLWEALGHHGRSRKQPHQACPKVRQILDIWASGCGVVSLHCFQHVVAVEAYTREACIVEALGIQTLTNQKQGHCYGVVAGWPPARRRRLGVHLLHRALLVFLAEGERQLHPQDIQARG

Target ORF information:

RefSeq Version	NM_152363.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152363.5

ORF Insert Sequence:

ATGTGGACCC CCGTGCGGCG CGGGGCAGCC TGGCTGGGAA CCCTGCTCGG CCCTGGAGAG 
GGGCCCCCCG AGCCGAGGGG AGGAGCGGCG CATGCCCACA AGCCCCCCGC GGGAAGTAGG 
AAATCGACCG ACCAGGCGGT GCGCTTCGGA CCCAGCCAGG GCATGTGCTC GGAGGCCCGC 
CTGGCTCGCA GGTTGCGGGA TGCGCTGCGG GAGGAGGAGC CGTGGGCAGT AGAGGAGCTG 
CTGCGCTGCG GCGCGGACCC TAATTTGGTG CTAGAGGACG GCGCAGCGGC TGTGCACTTG 
GCGGCCGGAG CCCGGCACCC GCGCGGCCTG CGTTGCCTCG GGGCCCTACT GCGCCAAGGC 
GGGGACCCCA ACGCTCGATC TGTCGAGGCA CTGACGCCGC TGCATGTGGC CGCCGCGTGG 
GGCTGCCGCC GCGGCCTGGA GCTGCTGCTG AGCCAAGGAG CGGACCCGGC GCTGCGCGAC 
CAGGACGGAC TCCGGCCGCT GGACCTGGCC CTGCAGCAGG GACACCTGGA GTGCGCGCGA 
GTCCTGCAGG ATCTCGACAC GCGGACCAGG ACCCGGACCC GGATCGGGGC AGAGACTCAG 
GAGCCCGAGC CTGCACCTGG CACCCCAGGC CTCTCTGGAC CTACCGATGA GACGCTGGAC 
TCCATAGCAC TCCAAAAGCA GCCATGCAGA GGTGACAACA GGGACATTGG CTTGGAGGCT 
GACCCAGGAC CCCCCAGCCT CCCTGTTCCC CTTGAAACTG TGGACAAACA TGGGAGCTCG 
GCGTCCCCTC CAGGGCACTG GGATTACAGC TCAGACGCCT CTTTCGTCAC AGCGGTTGAG 
GTCTCTGGAG CTGAGGACCC AGCCTCGGAC ACTCCCCCCT GGGCTGGGTC ATTGCCACCG 
ACCAGGCAGG GACTTCTGCA TGTTGTCCAT GCCAACCAGA GGGTACCTAG GTCTCAGGGC 
ACGGAGGCAG AACTGAATGC CCGTCTGCAG GCCCTGACTC TGACCCCACC AAATGCTGCT 
GGCTTCCAGT CCTCCCCTTC CTCCATGCCT CTCCTGGACA GGAGTCCAGC TCATAGCCCC 
CCACGGACAC CAACCCCTGG AGCTTCTGAC TGCCACTGCC TGTGGGAGCA CCAGACATCC 
ATTGATAGTG ACATGGCCAC GCTCTGGCTG ACAGAGGATG AGGCAAGCTC TACAGGTGGC 
AGGGAACCTG TCGGCCCTTG CCGGCACCTG CCAGTCTCCA CTGTGTCTGA CTTGGAGTTG 
CTGAAGGGAC TCCGAGCACT TGGTGAGAAT CCTCACCCCA TCACACCCTT CACCAGGCAG 
TTGTACCACC AGCAGCTGGA AGAAGCCCAG ATTGCTCCTG GCCCAGAGTT TTCAGGGCAC 
AGCCTAGAAC TGGCTGCAGC CCTGCGGACG GGCTGTATTC CAGATGTCCA GGCAGATGAA 
GACGCGCTGG CCCAGCAGTT TGAGCAGCCA GATCCTGCCA GGAGGTGGCG GGAGGGGGTC 
GTGAAGTCTA GCTTCACCTA TCTGCTGCTG GACCCCAGGG AGACTCAGGA CCTGCCAGCC 
CGAGCCTTCT CACTGACCCC AGCTGAGCGC CTTCAGACTT TCATCCGTGC CATCTTCTAC 
GTGGGCAAAG GGACGAGGGC CCGGCCATAT GTCCACCTCT GGGAGGCCCT TGGTCACCAT 
GGGCGGTCAA GAAAACAGCC CCACCAGGCC TGCCCCAAGG TGCGTCAGAT CTTGGACATC 
TGGGCCAGTG GTTGCGGTGT TGTGTCCCTA CATTGCTTCC AGCACGTGGT CGCTGTGGAG 
GCTTATACAC GGGAGGCGTG TATTGTGGAA GCCCTAGGGA TCCAGACGCT CACCAACCAG 
AAGCAAGGGC ACTGCTATGG AGTGGTGGCA GGCTGGCCAC CTGCTCGTCG CCGGCGCTTG 
GGGGTGCACC TGCTGCACCG TGCCCTCCTT GTCTTCCTGG CTGAAGGCGA GCGACAGCTT 
CATCCCCAGG ACATCCAGGC CCGGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE1 ( NM_001278445.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265374.1 Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu00061
Clone ID Related Accession (Same CDS sequence)	NM_001278445.1
Accession Version	NM_001278445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Homo sapiens(human)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK303628.1, AK096688.1 and AC010463.6. Transcript Variant: This variant (4) uses two alternate splice sites in the coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK092706.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCACAA GCCCCCCGCG GGAAGTAGGA AATCGACCGA CCAGGCGGTG CGCTTCGGAC 
CCAGCCAGGG CATGTGCTCG GAGGCCCGCC TGGCTCGCAG GTTGCGGGAT GCGCTGCGGG 
AGGAGGAGCC GTGTAGAGGA GCTGCTGCGC TGCGGCGCGG ACCCTAATTT GGTGCTAGAG 
GACGGCGCAG CGGCTGTGCA CTTGGCGGCC GGAGCCCGGC ACCCGCGCGG CCTGCGTTGC 
CTCGGGGCCC TACTGCGCCA AGGCGGGGAC CCCAACGCTC GATCTGTCGA GGCACTGACG 
CCGCTGCATG TGGCCGCCGC GTGGGGCTGC CGCCGCGGCC TGGAGCTGCT GCTGAGCCAA 
GGAGCGGACC CGGCGCTGCG CGACCAGGAC GGACTCCGGC CGCTGGACCT GGCCCTGCAG 
CAGGGACACC TGGAGTGCGC GCGAGTCCTG CAGGATCTCG ACACGCGGAC CAGGACCCGG 
ACCCGGATCG GGGCAGAGAC TCAGGAGCCC GAGCCTGCAC CTGGCACCCC AGGCCTCTCT 
GGACCTACCG ATGAGACGCT GGACTCCATA GCACTCCAAA AGCAGCCATG CAGAGGTGAC 
AACAGGGACA TTGGCTTGGA GGCTGACCCA GGACCCCCCA GCCTCCCTGT TCCCCTTGAA 
ACTGTGGACA AACATGGGAG CTCGGCGTCC CCTCCAGGGC ACTGGGATTA CAGCTCAGAC 
GCCTCTTTCG TCACAGCGGT TGAGGTCTCT GGAGCTGAGG ACCCAGCCTC GGACACTCCC 
CCCTGGGCTG GGTCATTGCC ACCGACCAGG CAGGGACTTC TGCATGTTGT CCATGCCAAC 
CAGAGGGTAC CTAGGTCTCA GGGCACGGAG GCAGAACTGA ATGCCCGTCT GCAGGCCCTG 
ACTCTGACCC CACCAAATGC TGCTGGCTTC CAGTCCTCCC CTTCCTCCAT GCCTCTCCTG 
GACAGGAGTC CAGCTCATAG CCCCCCACGG ACACCAACCC CTGGAGCTTC TGACTGCCAC 
TGCCTGTGGG AGCACCAGAC ATCCATTGAT AGTGACATGG CCACGCTCTG GCTGACAGAG 
GATGAGGCAA GCTCTACAGG TGGCAGGGAA CCTGTCGGCC CTTGCCGGCA CCTGCCAGTC 
TCCACTGTGT CTGACTTGGA GTTGCTGAAG GGACTCCGAG CACTTGGCCC AGAGTTTTCA 
GGGCACAGCC TAGAACTGGC TGCAGCCCTG CGGACGGGCT GTATTCCAGA TGTCCAGGCA 
GATGAAGACG CGCTGGCCCA GCAGTTTGAG CAGCCAGATC CTGCCAGGAG GTGGCGGGAG 
GGGGTCGTGA AGTCTAGCTT CACCTATCTG CTGCTGGACC CCAGGGAGAC TCAGGACCTG 
CCAGCCCGAG CCTTCTCACT GACCCCAGCT GAGCGCCTTC AGACTTTCAT CCGTGCCATC 
TTCTACGTGG GCAAAGGGAC GAGGGCCCGG CCATATGTCC ACCTCTGGGA GGCCCTTGGT 
CACCATGGGC GGTCAAGAAA ACAGCCCCAC CAGGCCTGCC CCAAGGTGCG TCAGATCTTG 
GACATCTGGG CCAGTGGTTG CGGTGTTGTG TCCCTACATT GCTTCCAGCA CGTGGTCGCT 
GTGGAGGCTT ATACACGGGA GGCGTGTATT GTGGAAGCCC TAGGGATCCA GACGCTCACC 
AACCAGAAGC AAGGGCACTG CTATGGAGTG GTGGCAGGCT GGCCACCTGC TCGTCGCCGG 
CGCTTGGGGG TGCACCTGCT GCACCGTGCC CTCCTTGTCT TCCTGGCTGA AGGCGAGCGA 
CAGCTTCATC CCCAGGACAT CCAGGCCCGG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265374.1
CDS	206..2041
Translation	MPTSPPREVGNRPTRRCASDPARACARRPAWLAGCGMRCGRRSRVEELLRCGADPNLVLEDGAAAVHLAAGARHPRGLRCLGALLRQGGDPNARSVEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGLRPLDLALQQGHLECARVLQDLDTRTRTRTRIGAETQEPEPAPGTPGLSGPTDETLDSIALQKQPCRGDNRDIGLEADPGPPSLPVPLETVDKHGSSASPPGHWDYSSDASFVTAVEVSGAEDPASDTPPWAGSLPPTRQGLLHVVHANQRVPRSQGTEAELNARLQALTLTPPNAAGFQSSPSSMPLLDRSPAHSPPRTPTPGASDCHCLWEHQTSIDSDMATLWLTEDEASSTGGREPVGPCRHLPVSTVSDLELLKGLRALGPEFSGHSLELAAALRTGCIPDVQADEDALAQQFEQPDPARRWREGVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKGTRARPYVHLWEALGHHGRSRKQPHQACPKVRQILDIWASGCGVVSLHCFQHVVAVEAYTREACIVEALGIQTLTNQKQGHCYGVVAGWPPARRRRLGVHLLHRALLVFLAEGERQLHPQDIQARG

Target ORF information:

RefSeq Version	NM_001278445.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278445.1

ORF Insert Sequence:

ATGCCCACAA GCCCCCCGCG GGAAGTAGGA AATCGACCGA CCAGGCGGTG CGCTTCGGAC 
CCAGCCAGGG CATGTGCTCG GAGGCCCGCC TGGCTCGCAG GTTGCGGGAT GCGCTGCGGG 
AGGAGGAGCC GTGTAGAGGA GCTGCTGCGC TGCGGCGCGG ACCCTAATTT GGTGCTAGAG 
GACGGCGCAG CGGCTGTGCA CTTGGCGGCC GGAGCCCGGC ACCCGCGCGG CCTGCGTTGC 
CTCGGGGCCC TACTGCGCCA AGGCGGGGAC CCCAACGCTC GATCTGTCGA GGCACTGACG 
CCGCTGCATG TGGCCGCCGC GTGGGGCTGC CGCCGCGGCC TGGAGCTGCT GCTGAGCCAA 
GGAGCGGACC CGGCGCTGCG CGACCAGGAC GGACTCCGGC CGCTGGACCT GGCCCTGCAG 
CAGGGACACC TGGAGTGCGC GCGAGTCCTG CAGGATCTCG ACACGCGGAC CAGGACCCGG 
ACCCGGATCG GGGCAGAGAC TCAGGAGCCC GAGCCTGCAC CTGGCACCCC AGGCCTCTCT 
GGACCTACCG ATGAGACGCT GGACTCCATA GCACTCCAAA AGCAGCCATG CAGAGGTGAC 
AACAGGGACA TTGGCTTGGA GGCTGACCCA GGACCCCCCA GCCTCCCTGT TCCCCTTGAA 
ACTGTGGACA AACATGGGAG CTCGGCGTCC CCTCCAGGGC ACTGGGATTA CAGCTCAGAC 
GCCTCTTTCG TCACAGCGGT TGAGGTCTCT GGAGCTGAGG ACCCAGCCTC GGACACTCCC 
CCCTGGGCTG GGTCATTGCC ACCGACCAGG CAGGGACTTC TGCATGTTGT CCATGCCAAC 
CAGAGGGTAC CTAGGTCTCA GGGCACGGAG GCAGAACTGA ATGCCCGTCT GCAGGCCCTG 
ACTCTGACCC CACCAAATGC TGCTGGCTTC CAGTCCTCCC CTTCCTCCAT GCCTCTCCTG 
GACAGGAGTC CAGCTCATAG CCCCCCACGG ACACCAACCC CTGGAGCTTC TGACTGCCAC 
TGCCTGTGGG AGCACCAGAC ATCCATTGAT AGTGACATGG CCACGCTCTG GCTGACAGAG 
GATGAGGCAA GCTCTACAGG TGGCAGGGAA CCTGTCGGCC CTTGCCGGCA CCTGCCAGTC 
TCCACTGTGT CTGACTTGGA GTTGCTGAAG GGACTCCGAG CACTTGGCCC AGAGTTTTCA 
GGGCACAGCC TAGAACTGGC TGCAGCCCTG CGGACGGGCT GTATTCCAGA TGTCCAGGCA 
GATGAAGACG CGCTGGCCCA GCAGTTTGAG CAGCCAGATC CTGCCAGGAG GTGGCGGGAG 
GGGGTCGTGA AGTCTAGCTT CACCTATCTG CTGCTGGACC CCAGGGAGAC TCAGGACCTG 
CCAGCCCGAG CCTTCTCACT GACCCCAGCT GAGCGCCTTC AGACTTTCAT CCGTGCCATC 
TTCTACGTGG GCAAAGGGAC GAGGGCCCGG CCATATGTCC ACCTCTGGGA GGCCCTTGGT 
CACCATGGGC GGTCAAGAAA ACAGCCCCAC CAGGCCTGCC CCAAGGTGCG TCAGATCTTG 
GACATCTGGG CCAGTGGTTG CGGTGTTGTG TCCCTACATT GCTTCCAGCA CGTGGTCGCT 
GTGGAGGCTT ATACACGGGA GGCGTGTATT GTGGAAGCCC TAGGGATCCA GACGCTCACC 
AACCAGAAGC AAGGGCACTG CTATGGAGTG GTGGCAGGCT GGCCACCTGC TCGTCGCCGG 
CGCTTGGGGG TGCACCTGCT GCACCGTGCC CTCCTTGTCT TCCTGGCTGA AGGCGAGCGA 
CAGCTTCATC CCCAGGACAT CCAGGCCCGG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE1 ( NM_001278443.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265372.1 Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 2, mRNA.

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CloneID	OHu11312
Clone ID Related Accession (Same CDS sequence)	NM_001278443.1
Accession Version	NM_001278443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-02
Organism	Homo sapiens(human)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK303628.1, AK096688.1 and AC010463.6. Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC125274.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGACCC CCGTGCGGCG CGGGGCAGCC TGGCTGGGAA CCCTGCTCGG CCCTGGAGAG 
GGGCCCCCCG AGCCGAGGGG AGGAGCGGCG CATGCCCACA AGCCCCCCGC GGGAAGTAGG 
AAATCGACCG ACCAGGCGGT GCGCTTCGGA CCCAGCCAGG GCATGTGCTC GGAGGCCCGC 
CTGGCTCGCA GGGCAGTAGA GGAGCTGCTG CGCTGCGGCG CGGACCCTAA TTTGGTGCTA 
GAGGACGGCG CAGCGGCTGT GCACTTGGCG GCCGGAGCCC GGCACCCGCG CGGCCTGCGT 
TGCCTCGGGG CCCTACTGCG CCAAGGCGGG GACCCCAACG CTCGATCTGT CGAGGCACTG 
ACGCCGCTGC ATGTGGCCGC CGCGTGGGGC TGCCGCCGCG GCCTGGAGCT GCTGCTGAGC 
CAAGGAGCGG ACCCGGCGCT GCGCGACCAG GACGGACTCC GGCCGCTGGA CCTGGCCCTG 
CAGCAGGGAC ACCTGGAGTG CGCGCGAGTC CTGCAGGATC TCGACACGCG GACCAGGACC 
CGGACCCGGA TCGGGGCAGA GACTCAGGAG CCCGAGCCTG CACCTGGCAC CCCAGGCCTC 
TCTGGACCTA CCGATGAGAC GCTGGACTCC ATAGCACTCC AAAAGCAGCC ATGCAGAGGT 
GACAACAGGG ACATTGGCTT GGAGGCTGAC CCAGGACCCC CCAGCCTCCC TGTTCCCCTT 
GAAACTGTGG ACAAACATGG GAGCTCGGCG TCCCCTCCAG GGCACTGGGA TTACAGCTCA 
GACGCCTCTT TCGTCACAGC GGTTGAGGTC TCTGGAGCTG AGGACCCAGC CTCGGACACT 
CCCCCCTGGG CTGGGTCATT GCCACCGACC AGGCAGGGAC TTCTGCATGT TGTCCATGCC 
AACCAGAGGG TACCTAGGTC TCAGGGCACG GAGGCAGAAC TGAATGCCCG TCTGCAGGCC 
CTGACTCTGA CCCCACCAAA TGCTGCTGGC TTCCAGTCCT CCCCTTCCTC CATGCCTCTC 
CTGGACAGGA GTCCAGCTCA TAGCCCCCCA CGGACACCAA CCCCTGGAGC TTCTGACTGC 
CACTGCCTGT GGGAGCACCA GACATCCATT GATAGTGACA TGGCCACGCT CTGGCTGACA 
GAGGATGAGG CAAGCTCTAC AGGTGGCAGG GAACCTGTCG GCCCTTGCCG GCACCTGCCA 
GTCTCCACTG TGTCTGACTT GGAGTTGCTG AAGGGACTCC GAGCACTTGG CCCAGAGTTT 
TCAGGGCACA GCCTAGAACT GGCTGCAGCC CTGCGGACGG GCTGTATTCC AGATGTCCAG 
GCAGATGAAG ACGCGCTGGC CCAGCAGTTT GAGCAGCCAG ATCCTGCCAG GAGGTGGCGG 
GAGGGGGTCG TGAAGTCTAG CTTCACCTAT CTGCTGCTGG ACCCCAGGGA GACTCAGGAC 
CTGCCAGCCC GAGCCTTCTC ACTGACCCCA GCTGAGCGCC TTCAGACTTT CATCCGTGCC 
ATCTTCTACG TGGGCAAAGG GACGAGGGCC CGGCCATATG TCCACCTCTG GGAGGCCCTT 
GGTCACCATG GGCGGTCAAG AAAACAGCCC CACCAGGCCT GCCCCAAGGT GCGTCAGATC 
TTGGACATCT GGGCCAGTGG TTGCGGTGTT GTGTCCCTAC ATTGCTTCCA GCACGTGGTC 
GCTGTGGAGG CTTATACACG GGAGGCGTGT ATTGTGGAAG CCCTAGGGAT CCAGACGCTC 
ACCAACCAGA AGCAAGGGCA CTGCTATGGA GTGGTGGCAG GCTGGCCACC TGCTCGTCGC 
CGGCGCTTGG GGGTGCACCT GCTGCACCGT GCCCTCCTTG TCTTCCTGGC TGAAGGCGAG 
CGACAGCTTC ATCCCCAGGA CATCCAGGCC CGGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265372.1
CDS	115..2013
Translation	MWTPVRRGAAWLGTLLGPGEGPPEPRGGAAHAHKPPAGSRKSTDQAVRFGPSQGMCSEARLARRAVEELLRCGADPNLVLEDGAAAVHLAAGARHPRGLRCLGALLRQGGDPNARSVEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGLRPLDLALQQGHLECARVLQDLDTRTRTRTRIGAETQEPEPAPGTPGLSGPTDETLDSIALQKQPCRGDNRDIGLEADPGPPSLPVPLETVDKHGSSASPPGHWDYSSDASFVTAVEVSGAEDPASDTPPWAGSLPPTRQGLLHVVHANQRVPRSQGTEAELNARLQALTLTPPNAAGFQSSPSSMPLLDRSPAHSPPRTPTPGASDCHCLWEHQTSIDSDMATLWLTEDEASSTGGREPVGPCRHLPVSTVSDLELLKGLRALGPEFSGHSLELAAALRTGCIPDVQADEDALAQQFEQPDPARRWREGVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKGTRARPYVHLWEALGHHGRSRKQPHQACPKVRQILDIWASGCGVVSLHCFQHVVAVEAYTREACIVEALGIQTLTNQKQGHCYGVVAGWPPARRRRLGVHLLHRALLVFLAEGERQLHPQDIQARG

Target ORF information:

RefSeq Version	NM_001278443.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278443.1

ORF Insert Sequence:

ATGTGGACCC CCGTGCGGCG CGGGGCAGCC TGGCTGGGAA CCCTGCTCGG CCCTGGAGAG 
GGGCCCCCCG AGCCGAGGGG AGGAGCGGCG CATGCCCACA AGCCCCCCGC GGGAAGTAGG 
AAATCGACCG ACCAGGCGGT GCGCTTCGGA CCCAGCCAGG GCATGTGCTC GGAGGCCCGC 
CTGGCTCGCA GGGCAGTAGA GGAGCTGCTG CGCTGCGGCG CGGACCCTAA TTTGGTGCTA 
GAGGACGGCG CAGCGGCTGT GCACTTGGCG GCCGGAGCCC GGCACCCGCG CGGCCTGCGT 
TGCCTCGGGG CCCTACTGCG CCAAGGCGGG GACCCCAACG CTCGATCTGT CGAGGCACTG 
ACGCCGCTGC ATGTGGCCGC CGCGTGGGGC TGCCGCCGCG GCCTGGAGCT GCTGCTGAGC 
CAAGGAGCGG ACCCGGCGCT GCGCGACCAG GACGGACTCC GGCCGCTGGA CCTGGCCCTG 
CAGCAGGGAC ACCTGGAGTG CGCGCGAGTC CTGCAGGATC TCGACACGCG GACCAGGACC 
CGGACCCGGA TCGGGGCAGA GACTCAGGAG CCCGAGCCTG CACCTGGCAC CCCAGGCCTC 
TCTGGACCTA CCGATGAGAC GCTGGACTCC ATAGCACTCC AAAAGCAGCC ATGCAGAGGT 
GACAACAGGG ACATTGGCTT GGAGGCTGAC CCAGGACCCC CCAGCCTCCC TGTTCCCCTT 
GAAACTGTGG ACAAACATGG GAGCTCGGCG TCCCCTCCAG GGCACTGGGA TTACAGCTCA 
GACGCCTCTT TCGTCACAGC GGTTGAGGTC TCTGGAGCTG AGGACCCAGC CTCGGACACT 
CCCCCCTGGG CTGGGTCATT GCCACCGACC AGGCAGGGAC TTCTGCATGT TGTCCATGCC 
AACCAGAGGG TACCTAGGTC TCAGGGCACG GAGGCAGAAC TGAATGCCCG TCTGCAGGCC 
CTGACTCTGA CCCCACCAAA TGCTGCTGGC TTCCAGTCCT CCCCTTCCTC CATGCCTCTC 
CTGGACAGGA GTCCAGCTCA TAGCCCCCCA CGGACACCAA CCCCTGGAGC TTCTGACTGC 
CACTGCCTGT GGGAGCACCA GACATCCATT GATAGTGACA TGGCCACGCT CTGGCTGACA 
GAGGATGAGG CAAGCTCTAC AGGTGGCAGG GAACCTGTCG GCCCTTGCCG GCACCTGCCA 
GTCTCCACTG TGTCTGACTT GGAGTTGCTG AAGGGACTCC GAGCACTTGG CCCAGAGTTT 
TCAGGGCACA GCCTAGAACT GGCTGCAGCC CTGCGGACGG GCTGTATTCC AGATGTCCAG 
GCAGATGAAG ACGCGCTGGC CCAGCAGTTT GAGCAGCCAG ATCCTGCCAG GAGGTGGCGG 
GAGGGGGTCG TGAAGTCTAG CTTCACCTAT CTGCTGCTGG ACCCCAGGGA GACTCAGGAC 
CTGCCAGCCC GAGCCTTCTC ACTGACCCCA GCTGAGCGCC TTCAGACTTT CATCCGTGCC 
ATCTTCTACG TGGGCAAAGG GACGAGGGCC CGGCCATATG TCCACCTCTG GGAGGCCCTT 
GGTCACCATG GGCGGTCAAG AAAACAGCCC CACCAGGCCT GCCCCAAGGT GCGTCAGATC 
TTGGACATCT GGGCCAGTGG TTGCGGTGTT GTGTCCCTAC ATTGCTTCCA GCACGTGGTC 
GCTGTGGAGG CTTATACACG GGAGGCGTGT ATTGTGGAAG CCCTAGGGAT CCAGACGCTC 
ACCAACCAGA AGCAAGGGCA CTGCTATGGA GTGGTGGCAG GCTGGCCACC TGCTCGTCGC 
CGGCGCTTGG GGGTGCACCT GCTGCACCGT GCCCTCCTTG TCTTCCTGGC TGAAGGCGAG 
CGACAGCTTC ATCCCCAGGA CATCCAGGCC CGGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE1 ( NM_152363.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_689576.6 Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 1, mRNA.

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CloneID	OHu107147
Clone ID Related Accession (Same CDS sequence)	NM_152363.6
Accession Version	NM_152363.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Homo sapiens(human)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK303628.1, AK096688.1 and AC010463.6. On Nov 9, 2018 this sequence version replaced NM_152363.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.280551.1, SRR1163657.66042.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004, SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000404085.7/ ENSP00000384008.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGCTCGG AGGCCCGCCT GGCTCGCAGG TTGCGGGATG CGCTGCGGGA GGAGGAGCCG 
TGGGCAGTAG AGGAGCTGCT GCGCTGCGGC GCGGACCCTA ATTTGGTGCT AGAGGACGGC 
GCAGCGGCTG TGCACTTGGC GGCCGGAGCC CGGCACCCGC GCGGCCTGCG TTGCCTCGGG 
GCCCTACTGC GCCAAGGCGG GGACCCCAAC GCTCGATCTG TCGAGGCACT GACGCCGCTG 
CATGTGGCCG CCGCGTGGGG CTGCCGCCGC GGCCTGGAGC TGCTGCTGAG CCAAGGAGCG 
GACCCGGCGC TGCGCGACCA GGACGGACTC CGGCCGCTGG ACCTGGCCCT GCAGCAGGGA 
CACCTGGAGT GCGCGCGAGT CCTGCAGGAT CTCGACACGC GGACCAGGAC CCGGACCCGG 
ATCGGGGCAG AGACTCAGGA GCCCGAGCCT GCACCTGGCA CCCCAGGCCT CTCTGGACCT 
ACCGATGAGA CGCTGGACTC CATAGCACTC CAAAAGCAGC CATGCAGAGG TGACAACAGG 
GACATTGGCT TGGAGGCTGA CCCAGGACCC CCCAGCCTCC CTGTTCCCCT TGAAACTGTG 
GACAAACATG GGAGCTCGGC GTCCCCTCCA GGGCACTGGG ATTACAGCTC AGACGCCTCT 
TTCGTCACAG CGGTTGAGGT CTCTGGAGCT GAGGACCCAG CCTCGGACAC TCCCCCCTGG 
GCTGGGTCAT TGCCACCGAC CAGGCAGGGA CTTCTGCATG TTGTCCATGC CAACCAGAGG 
GTACCTAGGT CTCAGGGCAC GGAGGCAGAA CTGAATGCCC GTCTGCAGGC CCTGACTCTG 
ACCCCACCAA ATGCTGCTGG CTTCCAGTCC TCCCCTTCCT CCATGCCTCT CCTGGACAGG 
AGTCCAGCTC ATAGCCCCCC ACGGACACCA ACCCCTGGAG CTTCTGACTG CCACTGCCTG 
TGGGAGCACC AGACATCCAT TGATAGTGAC ATGGCCACGC TCTGGCTGAC AGAGGATGAG 
GCAAGCTCTA CAGGTGGCAG GGAACCTGTC GGCCCTTGCC GGCACCTGCC AGTCTCCACT 
GTGTCTGACT TGGAGTTGCT GAAGGGACTC CGAGCACTTG GTGAGAATCC TCACCCCATC 
ACACCCTTCA CCAGGCAGTT GTACCACCAG CAGCTGGAAG AAGCCCAGAT TGCTCCTGGC 
CCAGAGTTTT CAGGGCACAG CCTAGAACTG GCTGCAGCCC TGCGGACGGG CTGTATTCCA 
GATGTCCAGG CAGATGAAGA CGCGCTGGCC CAGCAGTTTG AGCAGCCAGA TCCTGCCAGG 
AGGTGGCGGG AGGGGGTCGT GAAGTCTAGC TTCACCTATC TGCTGCTGGA CCCCAGGGAG 
ACTCAGGACC TGCCAGCCCG AGCCTTCTCA CTGACCCCAG CTGAGCGCCT TCAGACTTTC 
ATCCGTGCCA TCTTCTACGT GGGCAAAGGG ACGAGGGCCC GGCCATATGT CCACCTCTGG 
GAGGCCCTTG GTCACCATGG GCGGTCAAGA AAACAGCCCC ACCAGGCCTG CCCCAAGGTG 
CGTCAGATCT TGGACATCTG GGCCAGTGGT TGCGGTGTTG TGTCCCTACA TTGCTTCCAG 
CACGTGGTCG CTGTGGAGGC TTATACACGG GAGGCGTGTA TTGTGGAAGC CCTAGGGATC 
CAGACGCTCA CCAACCAGAA GCAAGGGCAC TGCTATGGAG TGGTGGCAGG CTGGCCACCT 
GCTCGTCGCC GGCGCTTGGG GGTGCACCTG CTGCACCGTG CCCTCCTTGT CTTCCTGGCT 
GAAGGCGAGC GACAGCTTCA TCCCCAGGAC ATCCAGGCCC GGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689576.6
CDS	21..1868
Translation	MCSEARLARRLRDALREEEPWAVEELLRCGADPNLVLEDGAAAVHLAAGARHPRGLRCLGALLRQGGDPNARSVEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGLRPLDLALQQGHLECARVLQDLDTRTRTRTRIGAETQEPEPAPGTPGLSGPTDETLDSIALQKQPCRGDNRDIGLEADPGPPSLPVPLETVDKHGSSASPPGHWDYSSDASFVTAVEVSGAEDPASDTPPWAGSLPPTRQGLLHVVHANQRVPRSQGTEAELNARLQALTLTPPNAAGFQSSPSSMPLLDRSPAHSPPRTPTPGASDCHCLWEHQTSIDSDMATLWLTEDEASSTGGREPVGPCRHLPVSTVSDLELLKGLRALGENPHPITPFTRQLYHQQLEEAQIAPGPEFSGHSLELAAALRTGCIPDVQADEDALAQQFEQPDPARRWREGVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKGTRARPYVHLWEALGHHGRSRKQPHQACPKVRQILDIWASGCGVVSLHCFQHVVAVEAYTREACIVEALGIQTLTNQKQGHCYGVVAGWPPARRRRLGVHLLHRALLVFLAEGERQLHPQDIQARG

Target ORF information:

RefSeq Version	NM_152363.6
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152363.6

ORF Insert Sequence:

ATGTGCTCGG AGGCCCGCCT GGCTCGCAGG TTGCGGGATG CGCTGCGGGA GGAGGAGCCG 
TGGGCAGTAG AGGAGCTGCT GCGCTGCGGC GCGGACCCTA ATTTGGTGCT AGAGGACGGC 
GCAGCGGCTG TGCACTTGGC GGCCGGAGCC CGGCACCCGC GCGGCCTGCG TTGCCTCGGG 
GCCCTACTGC GCCAAGGCGG GGACCCCAAC GCTCGATCTG TCGAGGCACT GACGCCGCTG 
CATGTGGCCG CCGCGTGGGG CTGCCGCCGC GGCCTGGAGC TGCTGCTGAG CCAAGGAGCG 
GACCCGGCGC TGCGCGACCA GGACGGACTC CGGCCGCTGG ACCTGGCCCT GCAGCAGGGA 
CACCTGGAGT GCGCGCGAGT CCTGCAGGAT CTCGACACGC GGACCAGGAC CCGGACCCGG 
ATCGGGGCAG AGACTCAGGA GCCCGAGCCT GCACCTGGCA CCCCAGGCCT CTCTGGACCT 
ACCGATGAGA CGCTGGACTC CATAGCACTC CAAAAGCAGC CATGCAGAGG TGACAACAGG 
GACATTGGCT TGGAGGCTGA CCCAGGACCC CCCAGCCTCC CTGTTCCCCT TGAAACTGTG 
GACAAACATG GGAGCTCGGC GTCCCCTCCA GGGCACTGGG ATTACAGCTC AGACGCCTCT 
TTCGTCACAG CGGTTGAGGT CTCTGGAGCT GAGGACCCAG CCTCGGACAC TCCCCCCTGG 
GCTGGGTCAT TGCCACCGAC CAGGCAGGGA CTTCTGCATG TTGTCCATGC CAACCAGAGG 
GTACCTAGGT CTCAGGGCAC GGAGGCAGAA CTGAATGCCC GTCTGCAGGC CCTGACTCTG 
ACCCCACCAA ATGCTGCTGG CTTCCAGTCC TCCCCTTCCT CCATGCCTCT CCTGGACAGG 
AGTCCAGCTC ATAGCCCCCC ACGGACACCA ACCCCTGGAG CTTCTGACTG CCACTGCCTG 
TGGGAGCACC AGACATCCAT TGATAGTGAC ATGGCCACGC TCTGGCTGAC AGAGGATGAG 
GCAAGCTCTA CAGGTGGCAG GGAACCTGTC GGCCCTTGCC GGCACCTGCC AGTCTCCACT 
GTGTCTGACT TGGAGTTGCT GAAGGGACTC CGAGCACTTG GTGAGAATCC TCACCCCATC 
ACACCCTTCA CCAGGCAGTT GTACCACCAG CAGCTGGAAG AAGCCCAGAT TGCTCCTGGC 
CCAGAGTTTT CAGGGCACAG CCTAGAACTG GCTGCAGCCC TGCGGACGGG CTGTATTCCA 
GATGTCCAGG CAGATGAAGA CGCGCTGGCC CAGCAGTTTG AGCAGCCAGA TCCTGCCAGG 
AGGTGGCGGG AGGGGGTCGT GAAGTCTAGC TTCACCTATC TGCTGCTGGA CCCCAGGGAG 
ACTCAGGACC TGCCAGCCCG AGCCTTCTCA CTGACCCCAG CTGAGCGCCT TCAGACTTTC 
ATCCGTGCCA TCTTCTACGT GGGCAAAGGG ACGAGGGCCC GGCCATATGT CCACCTCTGG 
GAGGCCCTTG GTCACCATGG GCGGTCAAGA AAACAGCCCC ACCAGGCCTG CCCCAAGGTG 
CGTCAGATCT TGGACATCTG GGCCAGTGGT TGCGGTGTTG TGTCCCTACA TTGCTTCCAG 
CACGTGGTCG CTGTGGAGGC TTATACACGG GAGGCGTGTA TTGTGGAAGC CCTAGGGATC 
CAGACGCTCA CCAACCAGAA GCAAGGGCAC TGCTATGGAG TGGTGGCAGG CTGGCCACCT 
GCTCGTCGCC GGCGCTTGGG GGTGCACCTG CTGCACCGTG CCCTCCTTGT CTTCCTGGCT 
GAAGGCGAGC GACAGCTTCA TCCCCAGGAC ATCCAGGCCC GGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE1 ( NM_001278445.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265374.2 Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 4, mRNA.

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CloneID	OHu107149
Clone ID Related Accession (Same CDS sequence)	NM_001278445.2
Accession Version	NM_001278445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Homo sapiens(human)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK303628.1, AK096688.1 and AC010463.6. On Nov 10, 2018 this sequence version replaced NM_001278445.1. Transcript Variant: This variant (4) uses two alternate splice sites in the coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK092706.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCTGCG GGAGGAGGAG CCGTGTAGAG GAGCTGCTGC GCTGCGGCGC GGACCCTAAT 
TTGGTGCTAG AGGACGGCGC AGCGGCTGTG CACTTGGCGG CCGGAGCCCG GCACCCGCGC 
GGCCTGCGTT GCCTCGGGGC CCTACTGCGC CAAGGCGGGG ACCCCAACGC TCGATCTGTC 
GAGGCACTGA CGCCGCTGCA TGTGGCCGCC GCGTGGGGCT GCCGCCGCGG CCTGGAGCTG 
CTGCTGAGCC AAGGAGCGGA CCCGGCGCTG CGCGACCAGG ACGGACTCCG GCCGCTGGAC 
CTGGCCCTGC AGCAGGGACA CCTGGAGTGC GCGCGAGTCC TGCAGGATCT CGACACGCGG 
ACCAGGACCC GGACCCGGAT CGGGGCAGAG ACTCAGGAGC CCGAGCCTGC ACCTGGCACC 
CCAGGCCTCT CTGGACCTAC CGATGAGACG CTGGACTCCA TAGCACTCCA AAAGCAGCCA 
TGCAGAGGTG ACAACAGGGA CATTGGCTTG GAGGCTGACC CAGGACCCCC CAGCCTCCCT 
GTTCCCCTTG AAACTGTGGA CAAACATGGG AGCTCGGCGT CCCCTCCAGG GCACTGGGAT 
TACAGCTCAG ACGCCTCTTT CGTCACAGCG GTTGAGGTCT CTGGAGCTGA GGACCCAGCC 
TCGGACACTC CCCCCTGGGC TGGGTCATTG CCACCGACCA GGCAGGGACT TCTGCATGTT 
GTCCATGCCA ACCAGAGGGT ACCTAGGTCT CAGGGCACGG AGGCAGAACT GAATGCCCGT 
CTGCAGGCCC TGACTCTGAC CCCACCAAAT GCTGCTGGCT TCCAGTCCTC CCCTTCCTCC 
ATGCCTCTCC TGGACAGGAG TCCAGCTCAT AGCCCCCCAC GGACACCAAC CCCTGGAGCT 
TCTGACTGCC ACTGCCTGTG GGAGCACCAG ACATCCATTG ATAGTGACAT GGCCACGCTC 
TGGCTGACAG AGGATGAGGC AAGCTCTACA GGTGGCAGGG AACCTGTCGG CCCTTGCCGG 
CACCTGCCAG TCTCCACTGT GTCTGACTTG GAGTTGCTGA AGGGACTCCG AGCACTTGGC 
CCAGAGTTTT CAGGGCACAG CCTAGAACTG GCTGCAGCCC TGCGGACGGG CTGTATTCCA 
GATGTCCAGG CAGATGAAGA CGCGCTGGCC CAGCAGTTTG AGCAGCCAGA TCCTGCCAGG 
AGGTGGCGGG AGGGGGTCGT GAAGTCTAGC TTCACCTATC TGCTGCTGGA CCCCAGGGAG 
ACTCAGGACC TGCCAGCCCG AGCCTTCTCA CTGACCCCAG CTGAGCGCCT TCAGACTTTC 
ATCCGTGCCA TCTTCTACGT GGGCAAAGGG ACGAGGGCCC GGCCATATGT CCACCTCTGG 
GAGGCCCTTG GTCACCATGG GCGGTCAAGA AAACAGCCCC ACCAGGCCTG CCCCAAGGTG 
CGTCAGATCT TGGACATCTG GGCCAGTGGT TGCGGTGTTG TGTCCCTACA TTGCTTCCAG 
CACGTGGTCG CTGTGGAGGC TTATACACGG GAGGCGTGTA TTGTGGAAGC CCTAGGGATC 
CAGACGCTCA CCAACCAGAA GCAAGGGCAC TGCTATGGAG TGGTGGCAGG CTGGCCACCT 
GCTCGTCGCC GGCGCTTGGG GGTGCACCTG CTGCACCGTG CCCTCCTTGT CTTCCTGGCT 
GAAGGCGAGC GACAGCTTCA TCCCCAGGAC ATCCAGGCCC GGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265374.2
CDS	58..1785
Translation	MRCGRRSRVEELLRCGADPNLVLEDGAAAVHLAAGARHPRGLRCLGALLRQGGDPNARSVEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGLRPLDLALQQGHLECARVLQDLDTRTRTRTRIGAETQEPEPAPGTPGLSGPTDETLDSIALQKQPCRGDNRDIGLEADPGPPSLPVPLETVDKHGSSASPPGHWDYSSDASFVTAVEVSGAEDPASDTPPWAGSLPPTRQGLLHVVHANQRVPRSQGTEAELNARLQALTLTPPNAAGFQSSPSSMPLLDRSPAHSPPRTPTPGASDCHCLWEHQTSIDSDMATLWLTEDEASSTGGREPVGPCRHLPVSTVSDLELLKGLRALGPEFSGHSLELAAALRTGCIPDVQADEDALAQQFEQPDPARRWREGVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKGTRARPYVHLWEALGHHGRSRKQPHQACPKVRQILDIWASGCGVVSLHCFQHVVAVEAYTREACIVEALGIQTLTNQKQGHCYGVVAGWPPARRRRLGVHLLHRALLVFLAEGERQLHPQDIQARG

Target ORF information:

RefSeq Version	NM_001278445.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278445.2

ORF Insert Sequence:

ATGCGCTGCG GGAGGAGGAG CCGTGTAGAG GAGCTGCTGC GCTGCGGCGC GGACCCTAAT 
TTGGTGCTAG AGGACGGCGC AGCGGCTGTG CACTTGGCGG CCGGAGCCCG GCACCCGCGC 
GGCCTGCGTT GCCTCGGGGC CCTACTGCGC CAAGGCGGGG ACCCCAACGC TCGATCTGTC 
GAGGCACTGA CGCCGCTGCA TGTGGCCGCC GCGTGGGGCT GCCGCCGCGG CCTGGAGCTG 
CTGCTGAGCC AAGGAGCGGA CCCGGCGCTG CGCGACCAGG ACGGACTCCG GCCGCTGGAC 
CTGGCCCTGC AGCAGGGACA CCTGGAGTGC GCGCGAGTCC TGCAGGATCT CGACACGCGG 
ACCAGGACCC GGACCCGGAT CGGGGCAGAG ACTCAGGAGC CCGAGCCTGC ACCTGGCACC 
CCAGGCCTCT CTGGACCTAC CGATGAGACG CTGGACTCCA TAGCACTCCA AAAGCAGCCA 
TGCAGAGGTG ACAACAGGGA CATTGGCTTG GAGGCTGACC CAGGACCCCC CAGCCTCCCT 
GTTCCCCTTG AAACTGTGGA CAAACATGGG AGCTCGGCGT CCCCTCCAGG GCACTGGGAT 
TACAGCTCAG ACGCCTCTTT CGTCACAGCG GTTGAGGTCT CTGGAGCTGA GGACCCAGCC 
TCGGACACTC CCCCCTGGGC TGGGTCATTG CCACCGACCA GGCAGGGACT TCTGCATGTT 
GTCCATGCCA ACCAGAGGGT ACCTAGGTCT CAGGGCACGG AGGCAGAACT GAATGCCCGT 
CTGCAGGCCC TGACTCTGAC CCCACCAAAT GCTGCTGGCT TCCAGTCCTC CCCTTCCTCC 
ATGCCTCTCC TGGACAGGAG TCCAGCTCAT AGCCCCCCAC GGACACCAAC CCCTGGAGCT 
TCTGACTGCC ACTGCCTGTG GGAGCACCAG ACATCCATTG ATAGTGACAT GGCCACGCTC 
TGGCTGACAG AGGATGAGGC AAGCTCTACA GGTGGCAGGG AACCTGTCGG CCCTTGCCGG 
CACCTGCCAG TCTCCACTGT GTCTGACTTG GAGTTGCTGA AGGGACTCCG AGCACTTGGC 
CCAGAGTTTT CAGGGCACAG CCTAGAACTG GCTGCAGCCC TGCGGACGGG CTGTATTCCA 
GATGTCCAGG CAGATGAAGA CGCGCTGGCC CAGCAGTTTG AGCAGCCAGA TCCTGCCAGG 
AGGTGGCGGG AGGGGGTCGT GAAGTCTAGC TTCACCTATC TGCTGCTGGA CCCCAGGGAG 
ACTCAGGACC TGCCAGCCCG AGCCTTCTCA CTGACCCCAG CTGAGCGCCT TCAGACTTTC 
ATCCGTGCCA TCTTCTACGT GGGCAAAGGG ACGAGGGCCC GGCCATATGT CCACCTCTGG 
GAGGCCCTTG GTCACCATGG GCGGTCAAGA AAACAGCCCC ACCAGGCCTG CCCCAAGGTG 
CGTCAGATCT TGGACATCTG GGCCAGTGGT TGCGGTGTTG TGTCCCTACA TTGCTTCCAG 
CACGTGGTCG CTGTGGAGGC TTATACACGG GAGGCGTGTA TTGTGGAAGC CCTAGGGATC 
CAGACGCTCA CCAACCAGAA GCAAGGGCAC TGCTATGGAG TGGTGGCAGG CTGGCCACCT 
GCTCGTCGCC GGCGCTTGGG GGTGCACCTG CTGCACCGTG CCCTCCTTGT CTTCCTGGCT 
GAAGGCGAGC GACAGCTTCA TCCCCAGGAC ATCCAGGCCC GGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE1 ( NM_001278444.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265373.2 Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 3, mRNA.

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CloneID	OHu107152
Clone ID Related Accession (Same CDS sequence)	NM_001278444.2
Accession Version	NM_001278444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK303628.1, AK299977.1 and AC010463.6. On Nov 10, 2018 this sequence version replaced NM_001278444.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region and lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (3) is shorter and contains a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK299977.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004, SAMEA2163105 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCTCGG AGGCCCGCCT GGCTCGCAGG TTGCGGGATG CGCTGCGGGA GGAGGAGCCG 
TGGGCAGTAG AGGAGCTGCT GCGCTGCGGC GCGGACCCTA ATTTGGTGCT AGAGGACGGC 
GCAGCGGCTG TGCACTTGGC GGCCGGAGCC CGGCACCCGC GCGGCCTGCG TTGCCTCGGG 
GCCCTACTGC GCCAAGGCGG GGACCCCAAC GCTCGATCTG TCGAGGCACT GACGCCGCTG 
CATGTGGCCG CCGCGTGGGG CTGCCGCCGC GGCCTGGAGC TGCTGCTGAG CCAAGGAGCG 
GACCCGGCGC TGCGCGACCA GGACGGACTC CGGCCGCTGG ACCTGGCCCT GCAGCAGGGA 
CACCTGGAGT GCGCGCGAGT CCTGCAGGAT CTCGACACGC GGACCAGGAC CCGGACCCGG 
ATCGGGGCAG AGACTCAGGA GCCCGAGCCT GCACCTGGCA CCCCAGGCCT CTCTGGACCT 
ACCGATGAGA CGCTGGACTC CATAGCACTC CAAAAGCAGC CATGCAGAGG TGACAACAGG 
GACATTGGCT TGGAGGCTGA CCCAGGACCC CCCAGCCTCC CTGTTCCCCT TGAAACTGTG 
GACAAACATG GGAGCTCGGC GTCCCCTCCA GGGCACTGGG ATTACAGCTC AGACGCCTCT 
TTCGTCACAG CGGTTGAGGT CTCTGGAGCT GAGGACCCAG CCTCGGACAC TCCCCCCTGG 
GCTGGGTCAT TGCCACCGAC CAGGCAGGGA CTTCTGCATG TTGTCCATGC CAACCAGAGG 
GTACCTAGGT CTCAGGGCAC GGAGGCAGAA CTGAATGCCC GTCTGCAGGC CCTGACTCTG 
ACCCCACCAA ATGCTGCTGG CTTCCAGTCC TCCCCTTCCT CCATGCCTCT CCTGGACAGG 
AGTCCAGCTC ATAGCCCCCC ACGGACACCA ACCCCTGGAG CTTCTGACTG CCACTGCCTG 
TGGGAGCACC AGACATCCAT TGATAGTGAC ATGGCCACGC TCTGGCTGAC AGAGGATGAG 
GCAAGCTCTA CAGGTGGCAG GGAACCTGTC GGCCCTTGCC GGCACCTGCC AGTCTCCACT 
GTGTCTGACT TGGAGTTGCT GAAGGGACTC CGAGCACTTG GCCCAGAGTT TTCAGGGCAC 
AGCCTAGAAC TGGCTGCAGC CCTGCGGACG GGCTGTATTC CAGATGTCCA GGCAGATGAA 
GACGCGCTGG CCCAGCAGTT TGAGCAGCCA GATCCTGCCA GGAGGTGGCG GGAGGGGGTC 
GTGAAGTCTA GCTTCACCTA TCTGCTGCTG GACCCCAGGG AGACTCAGGA CCTGCCAGCC 
CGAGCCTTCT CACTGACCCC AGCTGAGCGC CTTCAGACTT TCATCCGTGC CATCTTCTAC 
GTGGGCAAAG GGACGAGGGC CCGGCCATAT GTCCACCTCT GGGAGGCCCT TGGTCACCAT 
GGGCGGTCAA GAAAACAGGG ATCCAGACGC TCACCAACCA GAAGCAAGGG CACTGCTATG 
GAGTGGTGGC AGGCTGGCCA CCTGCTCGTC GCCGGCGCTT GGGGGTGCAC CTGCTGCACC 
GTGCCCTCCT TGTCTTCCTG GCTGAAGGCG AGCGACAGCT TCATCCCCAG GACATCCAGG 
CCCGGGGCTG AGTGCTGGGG AGTTGGCCAT CCAGCCTGGG GAGAAATGTT TGAATGTTCC 
AGCTGCCCCA TGCTGACAGC AGCCCCCATC TCTGGTTTCA GAAGGGGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT TTGTGTGTGT GTGTGTGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265373.2
CDS	21..1814
Translation	MCSEARLARRLRDALREEEPWAVEELLRCGADPNLVLEDGAAAVHLAAGARHPRGLRCLGALLRQGGDPNARSVEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGLRPLDLALQQGHLECARVLQDLDTRTRTRTRIGAETQEPEPAPGTPGLSGPTDETLDSIALQKQPCRGDNRDIGLEADPGPPSLPVPLETVDKHGSSASPPGHWDYSSDASFVTAVEVSGAEDPASDTPPWAGSLPPTRQGLLHVVHANQRVPRSQGTEAELNARLQALTLTPPNAAGFQSSPSSMPLLDRSPAHSPPRTPTPGASDCHCLWEHQTSIDSDMATLWLTEDEASSTGGREPVGPCRHLPVSTVSDLELLKGLRALGPEFSGHSLELAAALRTGCIPDVQADEDALAQQFEQPDPARRWREGVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKGTRARPYVHLWEALGHHGRSRKQGSRRSPTRSKGTAMEWWQAGHLLVAGAWGCTCCTVPSLSSWLKASDSFIPRTSRPGAECWGVGHPAWGEMFECSSCPMLTAAPISGFRRGVCVCVCVCVCVCVCLCVCVCV

Target ORF information:

RefSeq Version	NM_001278444.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278444.2

ORF Insert Sequence:

ATGTGCTCGG AGGCCCGCCT GGCTCGCAGG TTGCGGGATG CGCTGCGGGA GGAGGAGCCG 
TGGGCAGTAG AGGAGCTGCT GCGCTGCGGC GCGGACCCTA ATTTGGTGCT AGAGGACGGC 
GCAGCGGCTG TGCACTTGGC GGCCGGAGCC CGGCACCCGC GCGGCCTGCG TTGCCTCGGG 
GCCCTACTGC GCCAAGGCGG GGACCCCAAC GCTCGATCTG TCGAGGCACT GACGCCGCTG 
CATGTGGCCG CCGCGTGGGG CTGCCGCCGC GGCCTGGAGC TGCTGCTGAG CCAAGGAGCG 
GACCCGGCGC TGCGCGACCA GGACGGACTC CGGCCGCTGG ACCTGGCCCT GCAGCAGGGA 
CACCTGGAGT GCGCGCGAGT CCTGCAGGAT CTCGACACGC GGACCAGGAC CCGGACCCGG 
ATCGGGGCAG AGACTCAGGA GCCCGAGCCT GCACCTGGCA CCCCAGGCCT CTCTGGACCT 
ACCGATGAGA CGCTGGACTC CATAGCACTC CAAAAGCAGC CATGCAGAGG TGACAACAGG 
GACATTGGCT TGGAGGCTGA CCCAGGACCC CCCAGCCTCC CTGTTCCCCT TGAAACTGTG 
GACAAACATG GGAGCTCGGC GTCCCCTCCA GGGCACTGGG ATTACAGCTC AGACGCCTCT 
TTCGTCACAG CGGTTGAGGT CTCTGGAGCT GAGGACCCAG CCTCGGACAC TCCCCCCTGG 
GCTGGGTCAT TGCCACCGAC CAGGCAGGGA CTTCTGCATG TTGTCCATGC CAACCAGAGG 
GTACCTAGGT CTCAGGGCAC GGAGGCAGAA CTGAATGCCC GTCTGCAGGC CCTGACTCTG 
ACCCCACCAA ATGCTGCTGG CTTCCAGTCC TCCCCTTCCT CCATGCCTCT CCTGGACAGG 
AGTCCAGCTC ATAGCCCCCC ACGGACACCA ACCCCTGGAG CTTCTGACTG CCACTGCCTG 
TGGGAGCACC AGACATCCAT TGATAGTGAC ATGGCCACGC TCTGGCTGAC AGAGGATGAG 
GCAAGCTCTA CAGGTGGCAG GGAACCTGTC GGCCCTTGCC GGCACCTGCC AGTCTCCACT 
GTGTCTGACT TGGAGTTGCT GAAGGGACTC CGAGCACTTG GCCCAGAGTT TTCAGGGCAC 
AGCCTAGAAC TGGCTGCAGC CCTGCGGACG GGCTGTATTC CAGATGTCCA GGCAGATGAA 
GACGCGCTGG CCCAGCAGTT TGAGCAGCCA GATCCTGCCA GGAGGTGGCG GGAGGGGGTC 
GTGAAGTCTA GCTTCACCTA TCTGCTGCTG GACCCCAGGG AGACTCAGGA CCTGCCAGCC 
CGAGCCTTCT CACTGACCCC AGCTGAGCGC CTTCAGACTT TCATCCGTGC CATCTTCTAC 
GTGGGCAAAG GGACGAGGGC CCGGCCATAT GTCCACCTCT GGGAGGCCCT TGGTCACCAT 
GGGCGGTCAA GAAAACAGGG ATCCAGACGC TCACCAACCA GAAGCAAGGG CACTGCTATG 
GAGTGGTGGC AGGCTGGCCA CCTGCTCGTC GCCGGCGCTT GGGGGTGCAC CTGCTGCACC 
GTGCCCTCCT TGTCTTCCTG GCTGAAGGCG AGCGACAGCT TCATCCCCAG GACATCCAGG 
CCCGGGGCTG AGTGCTGGGG AGTTGGCCAT CCAGCCTGGG GAGAAATGTT TGAATGTTCC 
AGCTGCCCCA TGCTGACAGC AGCCCCCATC TCTGGTTTCA GAAGGGGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT TTGTGTGTGT GTGTGTGTGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE1 ( NM_001278443.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265372.2 Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 2, mRNA.

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CloneID	OHu107156
Clone ID Related Accession (Same CDS sequence)	NM_001278443.2
Accession Version	NM_001278443.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Homo sapiens(human)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK303628.1, AK096688.1 and AC010463.6. On Nov 10, 2018 this sequence version replaced NM_001278443.1. Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC125274.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCTCGG AGGCCCGCCT GGCTCGCAGG GCAGTAGAGG AGCTGCTGCG CTGCGGCGCG 
GACCCTAATT TGGTGCTAGA GGACGGCGCA GCGGCTGTGC ACTTGGCGGC CGGAGCCCGG 
CACCCGCGCG GCCTGCGTTG CCTCGGGGCC CTACTGCGCC AAGGCGGGGA CCCCAACGCT 
CGATCTGTCG AGGCACTGAC GCCGCTGCAT GTGGCCGCCG CGTGGGGCTG CCGCCGCGGC 
CTGGAGCTGC TGCTGAGCCA AGGAGCGGAC CCGGCGCTGC GCGACCAGGA CGGACTCCGG 
CCGCTGGACC TGGCCCTGCA GCAGGGACAC CTGGAGTGCG CGCGAGTCCT GCAGGATCTC 
GACACGCGGA CCAGGACCCG GACCCGGATC GGGGCAGAGA CTCAGGAGCC CGAGCCTGCA 
CCTGGCACCC CAGGCCTCTC TGGACCTACC GATGAGACGC TGGACTCCAT AGCACTCCAA 
AAGCAGCCAT GCAGAGGTGA CAACAGGGAC ATTGGCTTGG AGGCTGACCC AGGACCCCCC 
AGCCTCCCTG TTCCCCTTGA AACTGTGGAC AAACATGGGA GCTCGGCGTC CCCTCCAGGG 
CACTGGGATT ACAGCTCAGA CGCCTCTTTC GTCACAGCGG TTGAGGTCTC TGGAGCTGAG 
GACCCAGCCT CGGACACTCC CCCCTGGGCT GGGTCATTGC CACCGACCAG GCAGGGACTT 
CTGCATGTTG TCCATGCCAA CCAGAGGGTA CCTAGGTCTC AGGGCACGGA GGCAGAACTG 
AATGCCCGTC TGCAGGCCCT GACTCTGACC CCACCAAATG CTGCTGGCTT CCAGTCCTCC 
CCTTCCTCCA TGCCTCTCCT GGACAGGAGT CCAGCTCATA GCCCCCCACG GACACCAACC 
CCTGGAGCTT CTGACTGCCA CTGCCTGTGG GAGCACCAGA CATCCATTGA TAGTGACATG 
GCCACGCTCT GGCTGACAGA GGATGAGGCA AGCTCTACAG GTGGCAGGGA ACCTGTCGGC 
CCTTGCCGGC ACCTGCCAGT CTCCACTGTG TCTGACTTGG AGTTGCTGAA GGGACTCCGA 
GCACTTGGCC CAGAGTTTTC AGGGCACAGC CTAGAACTGG CTGCAGCCCT GCGGACGGGC 
TGTATTCCAG ATGTCCAGGC AGATGAAGAC GCGCTGGCCC AGCAGTTTGA GCAGCCAGAT 
CCTGCCAGGA GGTGGCGGGA GGGGGTCGTG AAGTCTAGCT TCACCTATCT GCTGCTGGAC 
CCCAGGGAGA CTCAGGACCT GCCAGCCCGA GCCTTCTCAC TGACCCCAGC TGAGCGCCTT 
CAGACTTTCA TCCGTGCCAT CTTCTACGTG GGCAAAGGGA CGAGGGCCCG GCCATATGTC 
CACCTCTGGG AGGCCCTTGG TCACCATGGG CGGTCAAGAA AACAGCCCCA CCAGGCCTGC 
CCCAAGGTGC GTCAGATCTT GGACATCTGG GCCAGTGGTT GCGGTGTTGT GTCCCTACAT 
TGCTTCCAGC ACGTGGTCGC TGTGGAGGCT TATACACGGG AGGCGTGTAT TGTGGAAGCC 
CTAGGGATCC AGACGCTCAC CAACCAGAAG CAAGGGCACT GCTATGGAGT GGTGGCAGGC 
TGGCCACCTG CTCGTCGCCG GCGCTTGGGG GTGCACCTGC TGCACCGTGC CCTCCTTGTC 
TTCCTGGCTG AAGGCGAGCG ACAGCTTCAT CCCCAGGACA TCCAGGCCCG GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265372.2
CDS	21..1757
Translation	MCSEARLARRAVEELLRCGADPNLVLEDGAAAVHLAAGARHPRGLRCLGALLRQGGDPNARSVEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGLRPLDLALQQGHLECARVLQDLDTRTRTRTRIGAETQEPEPAPGTPGLSGPTDETLDSIALQKQPCRGDNRDIGLEADPGPPSLPVPLETVDKHGSSASPPGHWDYSSDASFVTAVEVSGAEDPASDTPPWAGSLPPTRQGLLHVVHANQRVPRSQGTEAELNARLQALTLTPPNAAGFQSSPSSMPLLDRSPAHSPPRTPTPGASDCHCLWEHQTSIDSDMATLWLTEDEASSTGGREPVGPCRHLPVSTVSDLELLKGLRALGPEFSGHSLELAAALRTGCIPDVQADEDALAQQFEQPDPARRWREGVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKGTRARPYVHLWEALGHHGRSRKQPHQACPKVRQILDIWASGCGVVSLHCFQHVVAVEAYTREACIVEALGIQTLTNQKQGHCYGVVAGWPPARRRRLGVHLLHRALLVFLAEGERQLHPQDIQARG

Target ORF information:

RefSeq Version	NM_001278443.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278443.2

ORF Insert Sequence:

ATGTGCTCGG AGGCCCGCCT GGCTCGCAGG GCAGTAGAGG AGCTGCTGCG CTGCGGCGCG 
GACCCTAATT TGGTGCTAGA GGACGGCGCA GCGGCTGTGC ACTTGGCGGC CGGAGCCCGG 
CACCCGCGCG GCCTGCGTTG CCTCGGGGCC CTACTGCGCC AAGGCGGGGA CCCCAACGCT 
CGATCTGTCG AGGCACTGAC GCCGCTGCAT GTGGCCGCCG CGTGGGGCTG CCGCCGCGGC 
CTGGAGCTGC TGCTGAGCCA AGGAGCGGAC CCGGCGCTGC GCGACCAGGA CGGACTCCGG 
CCGCTGGACC TGGCCCTGCA GCAGGGACAC CTGGAGTGCG CGCGAGTCCT GCAGGATCTC 
GACACGCGGA CCAGGACCCG GACCCGGATC GGGGCAGAGA CTCAGGAGCC CGAGCCTGCA 
CCTGGCACCC CAGGCCTCTC TGGACCTACC GATGAGACGC TGGACTCCAT AGCACTCCAA 
AAGCAGCCAT GCAGAGGTGA CAACAGGGAC ATTGGCTTGG AGGCTGACCC AGGACCCCCC 
AGCCTCCCTG TTCCCCTTGA AACTGTGGAC AAACATGGGA GCTCGGCGTC CCCTCCAGGG 
CACTGGGATT ACAGCTCAGA CGCCTCTTTC GTCACAGCGG TTGAGGTCTC TGGAGCTGAG 
GACCCAGCCT CGGACACTCC CCCCTGGGCT GGGTCATTGC CACCGACCAG GCAGGGACTT 
CTGCATGTTG TCCATGCCAA CCAGAGGGTA CCTAGGTCTC AGGGCACGGA GGCAGAACTG 
AATGCCCGTC TGCAGGCCCT GACTCTGACC CCACCAAATG CTGCTGGCTT CCAGTCCTCC 
CCTTCCTCCA TGCCTCTCCT GGACAGGAGT CCAGCTCATA GCCCCCCACG GACACCAACC 
CCTGGAGCTT CTGACTGCCA CTGCCTGTGG GAGCACCAGA CATCCATTGA TAGTGACATG 
GCCACGCTCT GGCTGACAGA GGATGAGGCA AGCTCTACAG GTGGCAGGGA ACCTGTCGGC 
CCTTGCCGGC ACCTGCCAGT CTCCACTGTG TCTGACTTGG AGTTGCTGAA GGGACTCCGA 
GCACTTGGCC CAGAGTTTTC AGGGCACAGC CTAGAACTGG CTGCAGCCCT GCGGACGGGC 
TGTATTCCAG ATGTCCAGGC AGATGAAGAC GCGCTGGCCC AGCAGTTTGA GCAGCCAGAT 
CCTGCCAGGA GGTGGCGGGA GGGGGTCGTG AAGTCTAGCT TCACCTATCT GCTGCTGGAC 
CCCAGGGAGA CTCAGGACCT GCCAGCCCGA GCCTTCTCAC TGACCCCAGC TGAGCGCCTT 
CAGACTTTCA TCCGTGCCAT CTTCTACGTG GGCAAAGGGA CGAGGGCCCG GCCATATGTC 
CACCTCTGGG AGGCCCTTGG TCACCATGGG CGGTCAAGAA AACAGCCCCA CCAGGCCTGC 
CCCAAGGTGC GTCAGATCTT GGACATCTGG GCCAGTGGTT GCGGTGTTGT GTCCCTACAT 
TGCTTCCAGC ACGTGGTCGC TGTGGAGGCT TATACACGGG AGGCGTGTAT TGTGGAAGCC 
CTAGGGATCC AGACGCTCAC CAACCAGAAG CAAGGGCACT GCTATGGAGT GGTGGCAGGC 
TGGCCACCTG CTCGTCGCCG GCGCTTGGGG GTGCACCTGC TGCACCGTGC CCTCCTTGTC 
TTCCTGGCTG AAGGCGAGCG ACAGCTTCAT CCCCAGGACA TCCAGGCCCG GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE1 ( NM_001278444.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265373.1 Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu11133
Clone ID Related Accession (Same CDS sequence)	NM_001278444.1
Accession Version	NM_001278444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Homo sapiens(human)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK303628.1, AK299977.1 and AC010463.6. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region and lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (3) is shorter and contains a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK299977.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004, SAMEA2163105 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGACCC CCGTGCGGCG CGGGGCAGCC TGGCTGGGAA CCCTGCTCGG CCCTGGAGAG 
GGGCCCCCCG AGCCGAGGGG AGGAGCGGCG CATGCCCACA AGCCCCCCGC GGGAAGTAGG 
AAATCGACCG ACCAGGCGGT GCGCTTCGGA CCCAGCCAGG GCATGTGCTC GGAGGCCCGC 
CTGGCTCGCA GGTTGCGGGA TGCGCTGCGG GAGGAGGAGC CGTGGGCAGT AGAGGAGCTG 
CTGCGCTGCG GCGCGGACCC TAATTTGGTG CTAGAGGACG GCGCAGCGGC TGTGCACTTG 
GCGGCCGGAG CCCGGCACCC GCGCGGCCTG CGTTGCCTCG GGGCCCTACT GCGCCAAGGC 
GGGGACCCCA ACGCTCGATC TGTCGAGGCA CTGACGCCGC TGCATGTGGC CGCCGCGTGG 
GGCTGCCGCC GCGGCCTGGA GCTGCTGCTG AGCCAAGGAG CGGACCCGGC GCTGCGCGAC 
CAGGACGGAC TCCGGCCGCT GGACCTGGCC CTGCAGCAGG GACACCTGGA GTGCGCGCGA 
GTCCTGCAGG ATCTCGACAC GCGGACCAGG ACCCGGACCC GGATCGGGGC AGAGACTCAG 
GAGCCCGAGC CTGCACCTGG CACCCCAGGC CTCTCTGGAC CTACCGATGA GACGCTGGAC 
TCCATAGCAC TCCAAAAGCA GCCATGCAGA GGTGACAACA GGGACATTGG CTTGGAGGCT 
GACCCAGGAC CCCCCAGCCT CCCTGTTCCC CTTGAAACTG TGGACAAACA TGGGAGCTCG 
GCGTCCCCTC CAGGGCACTG GGATTACAGC TCAGACGCCT CTTTCGTCAC AGCGGTTGAG 
GTCTCTGGAG CTGAGGACCC AGCCTCGGAC ACTCCCCCCT GGGCTGGGTC ATTGCCACCG 
ACCAGGCAGG GACTTCTGCA TGTTGTCCAT GCCAACCAGA GGGTACCTAG GTCTCAGGGC 
ACGGAGGCAG AACTGAATGC CCGTCTGCAG GCCCTGACTC TGACCCCACC AAATGCTGCT 
GGCTTCCAGT CCTCCCCTTC CTCCATGCCT CTCCTGGACA GGAGTCCAGC TCATAGCCCC 
CCACGGACAC CAACCCCTGG AGCTTCTGAC TGCCACTGCC TGTGGGAGCA CCAGACATCC 
ATTGATAGTG ACATGGCCAC GCTCTGGCTG ACAGAGGATG AGGCAAGCTC TACAGGTGGC 
AGGGAACCTG TCGGCCCTTG CCGGCACCTG CCAGTCTCCA CTGTGTCTGA CTTGGAGTTG 
CTGAAGGGAC TCCGAGCACT TGGCCCAGAG TTTTCAGGGC ACAGCCTAGA ACTGGCTGCA 
GCCCTGCGGA CGGGCTGTAT TCCAGATGTC CAGGCAGATG AAGACGCGCT GGCCCAGCAG 
TTTGAGCAGC CAGATCCTGC CAGGAGGTGG CGGGAGGGGG TCGTGAAGTC TAGCTTCACC 
TATCTGCTGC TGGACCCCAG GGAGACTCAG GACCTGCCAG CCCGAGCCTT CTCACTGACC 
CCAGCTGAGC GCCTTCAGAC TTTCATCCGT GCCATCTTCT ACGTGGGCAA AGGGACGAGG 
GCCCGGCCAT ATGTCCACCT CTGGGAGGCC CTTGGTCACC ATGGGCGGTC AAGAAAACAG 
GGATCCAGAC GCTCACCAAC CAGAAGCAAG GGCACTGCTA TGGAGTGGTG GCAGGCTGGC 
CACCTGCTCG TCGCCGGCGC TTGGGGGTGC ACCTGCTGCA CCGTGCCCTC CTTGTCTTCC 
TGGCTGAAGG CGAGCGACAG CTTCATCCCC AGGACATCCA GGCCCGGGGC TGAGTGCTGG 
GGAGTTGGCC ATCCAGCCTG GGGAGAAATG TTTGAATGTT CCAGCTGCCC CATGCTGACA 
GCAGCCCCCA TCTCTGGTTT CAGAAGGGGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTTTGTGTGT GTGTGTGTGT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265373.1
CDS	115..2070
Translation	MWTPVRRGAAWLGTLLGPGEGPPEPRGGAAHAHKPPAGSRKSTDQAVRFGPSQGMCSEARLARRLRDALREEEPWAVEELLRCGADPNLVLEDGAAAVHLAAGARHPRGLRCLGALLRQGGDPNARSVEALTPLHVAAAWGCRRGLELLLSQGADPALRDQDGLRPLDLALQQGHLECARVLQDLDTRTRTRTRIGAETQEPEPAPGTPGLSGPTDETLDSIALQKQPCRGDNRDIGLEADPGPPSLPVPLETVDKHGSSASPPGHWDYSSDASFVTAVEVSGAEDPASDTPPWAGSLPPTRQGLLHVVHANQRVPRSQGTEAELNARLQALTLTPPNAAGFQSSPSSMPLLDRSPAHSPPRTPTPGASDCHCLWEHQTSIDSDMATLWLTEDEASSTGGREPVGPCRHLPVSTVSDLELLKGLRALGPEFSGHSLELAAALRTGCIPDVQADEDALAQQFEQPDPARRWREGVVKSSFTYLLLDPRETQDLPARAFSLTPAERLQTFIRAIFYVGKGTRARPYVHLWEALGHHGRSRKQGSRRSPTRSKGTAMEWWQAGHLLVAGAWGCTCCTVPSLSSWLKASDSFIPRTSRPGAECWGVGHPAWGEMFECSSCPMLTAAPISGFRRGVCVCVCVCVCVCVCLCVCVCV

Target ORF information:

RefSeq Version	NM_001278444.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and LEM domain containing 1 (ANKLE1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278444.1

ORF Insert Sequence:

ATGTGGACCC CCGTGCGGCG CGGGGCAGCC TGGCTGGGAA CCCTGCTCGG CCCTGGAGAG 
GGGCCCCCCG AGCCGAGGGG AGGAGCGGCG CATGCCCACA AGCCCCCCGC GGGAAGTAGG 
AAATCGACCG ACCAGGCGGT GCGCTTCGGA CCCAGCCAGG GCATGTGCTC GGAGGCCCGC 
CTGGCTCGCA GGTTGCGGGA TGCGCTGCGG GAGGAGGAGC CGTGGGCAGT AGAGGAGCTG 
CTGCGCTGCG GCGCGGACCC TAATTTGGTG CTAGAGGACG GCGCAGCGGC TGTGCACTTG 
GCGGCCGGAG CCCGGCACCC GCGCGGCCTG CGTTGCCTCG GGGCCCTACT GCGCCAAGGC 
GGGGACCCCA ACGCTCGATC TGTCGAGGCA CTGACGCCGC TGCATGTGGC CGCCGCGTGG 
GGCTGCCGCC GCGGCCTGGA GCTGCTGCTG AGCCAAGGAG CGGACCCGGC GCTGCGCGAC 
CAGGACGGAC TCCGGCCGCT GGACCTGGCC CTGCAGCAGG GACACCTGGA GTGCGCGCGA 
GTCCTGCAGG ATCTCGACAC GCGGACCAGG ACCCGGACCC GGATCGGGGC AGAGACTCAG 
GAGCCCGAGC CTGCACCTGG CACCCCAGGC CTCTCTGGAC CTACCGATGA GACGCTGGAC 
TCCATAGCAC TCCAAAAGCA GCCATGCAGA GGTGACAACA GGGACATTGG CTTGGAGGCT 
GACCCAGGAC CCCCCAGCCT CCCTGTTCCC CTTGAAACTG TGGACAAACA TGGGAGCTCG 
GCGTCCCCTC CAGGGCACTG GGATTACAGC TCAGACGCCT CTTTCGTCAC AGCGGTTGAG 
GTCTCTGGAG CTGAGGACCC AGCCTCGGAC ACTCCCCCCT GGGCTGGGTC ATTGCCACCG 
ACCAGGCAGG GACTTCTGCA TGTTGTCCAT GCCAACCAGA GGGTACCTAG GTCTCAGGGC 
ACGGAGGCAG AACTGAATGC CCGTCTGCAG GCCCTGACTC TGACCCCACC AAATGCTGCT 
GGCTTCCAGT CCTCCCCTTC CTCCATGCCT CTCCTGGACA GGAGTCCAGC TCATAGCCCC 
CCACGGACAC CAACCCCTGG AGCTTCTGAC TGCCACTGCC TGTGGGAGCA CCAGACATCC 
ATTGATAGTG ACATGGCCAC GCTCTGGCTG ACAGAGGATG AGGCAAGCTC TACAGGTGGC 
AGGGAACCTG TCGGCCCTTG CCGGCACCTG CCAGTCTCCA CTGTGTCTGA CTTGGAGTTG 
CTGAAGGGAC TCCGAGCACT TGGCCCAGAG TTTTCAGGGC ACAGCCTAGA ACTGGCTGCA 
GCCCTGCGGA CGGGCTGTAT TCCAGATGTC CAGGCAGATG AAGACGCGCT GGCCCAGCAG 
TTTGAGCAGC CAGATCCTGC CAGGAGGTGG CGGGAGGGGG TCGTGAAGTC TAGCTTCACC 
TATCTGCTGC TGGACCCCAG GGAGACTCAG GACCTGCCAG CCCGAGCCTT CTCACTGACC 
CCAGCTGAGC GCCTTCAGAC TTTCATCCGT GCCATCTTCT ACGTGGGCAA AGGGACGAGG 
GCCCGGCCAT ATGTCCACCT CTGGGAGGCC CTTGGTCACC ATGGGCGGTC AAGAAAACAG 
GGATCCAGAC GCTCACCAAC CAGAAGCAAG GGCACTGCTA TGGAGTGGTG GCAGGCTGGC 
CACCTGCTCG TCGCCGGCGC TTGGGGGTGC ACCTGCTGCA CCGTGCCCTC CTTGTCTTCC 
TGGCTGAAGG CGAGCGACAG CTTCATCCCC AGGACATCCA GGCCCGGGGC TGAGTGCTGG 
GGAGTTGGCC ATCCAGCCTG GGGAGAAATG TTTGAATGTT CCAGCTGCCC CATGCTGACA 
GCAGCCCCCA TCTCTGGTTT CAGAAGGGGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTTTGTGTGT GTGTGTGTGT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of breast cancer associated variants that modulate transcription factor binding.
PLoS genetics13(9)e1006761(2017 Sep)
Liu Y,Walavalkar NM,Dozmorov MG,Rich SS,Civelek M,Guertin MJ

Cis-eQTL-based trans-ethnic meta-analysis reveals novel genes associated with breast cancer risk.
PLoS genetics13(3)e1006690(2017 Mar)
Hoffman JD,Graff RE,Emami NC,Tai CG,Passarelli MN,Hu D,Huntsman S,Hadley D,Leong L,Majumdar A,Zaitlen N,Ziv E,Witte JS

Nucleo-cytoplasmic shuttling of the endonuclease ankyrin repeats and LEM domain-containing protein 1 (Ankle1) is mediated by canonical nuclear export- and nuclear import signals.
BMC cell biology17(1)23(2016 06)
Zlopasa L,Brachner A,Foisner R

Genome-wide association study identifies 25 known breast cancer susceptibility loci as risk factors for triple-negative breast cancer.
Carcinogenesis35(5)1012-9(2014 May)
Purrington KS,Slager S,Eccles D,Yannoukakos D,Fasching PA,Miron P,Carpenter J,Chang-Claude J,Martin NG,Montgomery GW,Kristensen V,Anton-Culver H,Goodfellow P,Tapper WJ,Rafiq S,Gerty SM,Durcan L,Konstantopoulou I,Fostira F,Vratimos A,Apostolou P,Konstanta I,Kotoula V,Lakis S,Dimopoulos MA,Skarlos D,Pectasides D,Fountzilas G,Beckmann MW,Hein A,Ruebner M,Ekici AB,Hartmann A,Schulz-Wendtland R,Renner SP,Janni W,Rack B,Scholz C,Neugebauer J,Andergassen U,Lux MP,Haeberle L,Clarke C,Pathmanathan N,Rudolph A,Flesch-Janys D,Nickels S,Olson JE,Ingle JN,Olswold C,Slettedahl S,Eckel-Passow JE,Anderson SK,Visscher DW,Cafourek VL,Sicotte H,Prodduturi N,Weiderpass E,Bernstein L,Ziogas A,Ivanovich J,Giles GG,Baglietto L,Southey M,Kosma VM,Fischer HP,,Reed MW,Cross SS,Deming-Halverson S,Shrubsole M,Cai Q,Shu XO,Daly M,Weaver J,Ross E,Klemp J,Sharma P,Torres D,R?diger T,W?lfing H,Ulmer HU,F?rsti A,Khoury T,Kumar S,Pilarski R,Shapiro CL,Greco D,Heikkil? P,Aittom?ki K,Blomqvist C,Irwanto A,Liu J,Pankratz VS,Wang X,Severi G,Mannermaa A,Easton D,Hall P,Brauch H,Cox A,Zheng W,Godwin AK,Hamann U,Ambrosone C,Toland AE,Nevanlinna H,Vachon CM,Couch FJ

A meta-analysis of genome-wide association studies of breast cancer identifies two novel susceptibility loci at 6q14 and 20q11.
Human molecular genetics21(24)5373-84(2012 Dec)
Siddiq A,Couch FJ,Chen GK,Lindstr?m S,Eccles D,Millikan RC,Michailidou K,Stram DO,Beckmann L,Rhie SK,Ambrosone CB,Aittom?ki K,Amiano P,Apicella C,,Baglietto L,Bandera EV,Beckmann MW,Berg CD,Bernstein L,Blomqvist C,Brauch H,Brinton L,Bui QM,Buring JE,Buys SS,Campa D,Carpenter JE,Chasman DI,Chang-Claude J,Chen C,Clavel-Chapelon F,Cox A,Cross SS,Czene K,Deming SL,Diasio RB,Diver WR,Dunning AM,Durcan L,Ekici AB,Fasching PA,,Feigelson HS,Fejerman L,Figueroa JD,Fletcher O,Flesch-Janys D,Gaudet MM,,Gerty SM,Rodriguez-Gil JL,Giles GG,van Gils CH,Godwin AK,Graham N,Greco D,Hall P,Hankinson SE,Hartmann A,Hein R,Heinz J,Hoover RN,Hopper JL,Hu JJ,Huntsman S,Ingles SA,Irwanto A,Isaacs C,Jacobs KB,John EM,Justenhoven C,Kaaks R,Kolonel LN,Coetzee GA,Lathrop M,Le Marchand L,Lee AM,Lee IM,Lesnick T,Lichtner P,Liu J,Lund E,Makalic E,Martin NG,McLean CA,Meijers-Heijboer H,Meindl A,Miron P,Monroe KR,Montgomery GW,M?ller-Myhsok B,Nickels S,Nyante SJ,Olswold C,Overvad K,Palli D,Park DJ,Palmer JR,Pathak H,Peto J,Pharoah P,Rahman N,Rivadeneira F,Schmidt DF,Schmutzler RK,Slager S,Southey MC,Stevens KN,Sinn HP,Press MF,Ross E,Riboli E,Ridker PM,Schumacher FR,Severi G,Dos Santos Silva I,Stone J,Sund M,Tapper WJ,Thun MJ,Travis RC,Turnbull C,Uitterlinden AG,Waisfisz Q,Wang X,Wang Z,Weaver J,Schulz-Wendtland R,Wilkens LR,Van Den Berg D,Zheng W,Ziegler RG,Ziv E,Nevanlinna H,Easton DF,Hunter DJ,Henderson BE,Chanock SJ,Garcia-Closas M,Kraft P,Haiman CA,Vachon CM

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ANKLE1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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