EARS2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EARS2
Entrez Gene ID	124454
Full Name	glutamyl-tRNA synthetase 2, mitochondrial
Synonyms	COXPD12,MSE1,gluRS
General protein information	Preferred Names glutamyl-tRNA synthetase 2, mitochondrial Names probable glutamate--tRNA ligase, mitochondrial glutamate tRNA ligase 2, mitochondrial glutamate--tRNA ligase probable glutamyl-tRNA synthetase, mitochondrial
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p12.2
Summary	This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of glutamate to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 12 (COXPD12). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015].
Disorder MIM:	612799
Disorder Html:	Combined oxidative phosphorylation deficiency 12, 614924 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001083614.1	NP_001077083.1	probable glutamate--tRNA ligase, mitochondrial isoform 1
XM_011545739.1	XP_011544041.1	probable glutamate--tRNA ligase, mitochondrial isoform X2
NM_001083614.2	NP_001077083.1	probable glutamate--tRNA ligase, mitochondrial isoform 1
XM_011545738.1	XP_011544040.1	probable glutamate--tRNA ligase, mitochondrial isoform X1
NM_001308211.1	NP_001295140.1	probable glutamate--tRNA ligase, mitochondrial isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	EARS2	NP_001077083.1
	EARS2	XP_001094523.1
Drosophila melanogaster (fruit fly)	CG4573	NP_648894.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002570	XP_312366.5
Saccharomyces cerevisiae (baker's yeast)	MSE1	NP_014609.1
Oryza sativa (rice)	Os02g0121000	NP_001045714.1
Xenopus tropicalis (tropical clawed frog)	ears2	NP_001008042.1
Canis lupus familiaris (dog)	EARS2	XP_005621472.1
Bos taurus (cattle)	EARS2	XP_002698070.2
Kluyveromyces lactis	KLLA0F07975g	XP_455441.1
Rattus norvegicus (Norway rat)	Ears2	NP_001152965.1
Gallus gallus (chicken)	EARS2	NP_001026638.1
Arabidopsis thaliana (thale cress)	ERS	NP_201210.1
Mus musculus (house mouse)	Ears2	NP_080416.1
Eremothecium gossypii	AGOS_AEL338C	NP_984522.1
Magnaporthe oryzae (rice blast fungus)	MGG_05275	XP_003712834.1
Pan troglodytes (chimpanzee)	EARS2	XP_001162700.1
Rattus norvegicus (Norway rat)	Ears2l1	XP_006231368.1
Danio rerio (zebrafish)	zgc:153247	NP_001038907.1
Caenorhabditis elegans (roundworm)	ears-2	NP_499917.2
Schizosaccharomyces pombe (fission yeast)	mse1	NP_593525.1

Gene Ontology
Bibliography

Related articles in PubMed

Multisystem fatal infantile disease caused by a novel homozygous EARS2 mutation.
Talim B, Pyle A, Griffin H, Topaloglu H, Tokatli A, Keogh MJ, Santibanez-Koref M, Chinnery PF, Horvath R
Brain : a journal of neurology136(Pt 2)e228(2013 Feb)

Leukoencephalopathy with thalamus and brainstem involvement and high lactate 'LTBL' caused by EARS2 mutations.
Steenweg ME, Ghezzi D, Haack T, Abbink TE, Martinelli D, van Berkel CG, Bley A, Diogo L, Grillo E, Te Water Naud? J, Strom TM, Bertini E, Prokisch H, van der Knaap MS, Zeviani M
Brain : a journal of neurology135(Pt 5)1387-94(2012 May)

Molecular diagnosis of infantile mitochondrial disease with targeted next-generation sequencing.
Calvo SE, Compton AG, Hershman SG, Lim SC, Lieber DS, Tucker EJ, Laskowski A, Garone C, Liu S, Jaffe DB, Christodoulou J, Fletcher JM, Bruno DL, Goldblatt J, Dimauro S, Thorburn DR, Mootha VK
Science translational medicine4(118)118ra10(2012 Jan)

Biogenesis of glutaminyl-mt tRNAGln in human mitochondria.
Nagao A, Suzuki T, Katoh T, Sakaguchi Y, Suzuki T
Proceedings of the National Academy of Sciences of the United States of America106(38)16209-14(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study presented that a new neurological disease, early-onset leukoencephalopathy with thalamus and brainstem involvement and high lactate, defined by a peculiar biphasic clinical course and caused by mutations in a single gene, EARS2.
Title: Leukoencephalopathy with thalamus and brainstem involvement and high lactate 'LTBL' caused by EARS2 mutations.

Strong candidate gene for mitochondrial disease, based on recessive mutations detected in infantile patients
Title: Molecular diagnosis of infantile mitochondrial disease with targeted next-generation sequencing.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Studies showed in vitro Gln-tRNA(Gln) formation catalyzed by the recombinant mtGluRS and hGatCAB.
Title: Biogenesis of glutaminyl-mt tRNAGln in human mitochondria.

The following EARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu49080	NM_001308211.1 Latest version!	Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu25880	NM_001083614.2 Latest version!	Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu75297	XM_011545738.1 Latest version!	Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu75298	XM_011545739.1 Latest version!	Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu25880	NM_001083614.1	Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EARS2 ( NM_001308211.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001295140.1 Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu49080
Clone ID Related Accession (Same CDS sequence)	NM_001308211.1
Accession Version	NM_001308211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	probable glutamate--tRNA ligase, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA692403.1, BC040013.1 and AC002400.1. On Apr 28, 2015 this sequence version replaced XM_006721020.1. Transcript Variant: This variant (3) an exon and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC040013.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC TCCTGAGGAG ACTGCTGCAG CGCGAGAGGC CTTCGGCGGC CTCTGGCCGC 
CCCGTAGGAC GGCGCGAGGC CAACCTGGGC ACTGATGCCG GGGTTGCGGT GCGAGTGCGG 
TTCGCTCCCA GCCCCACAGG CTTCTTGCAC CTGGGTGGCC TCCGCACTGC CTTGTACAAC 
TACATCTTTG CTAAGAAGTA CCAGGGGAGC TTCATCCTGA GGCTAGAGGA CACAGATCAG 
ACTCGCGTTG TGCCTGGGGC AGCGGAGAAT ATTGAGGACA TGCTGGAGTG GGCAGGCATC 
CCGCCTGATG AGAGCCCCCG CCGGGGCGGT CCTGCTGGGC CCTACCAGCA ATCTCAGCGG 
TTGGAGCTGT ATGCCCAGGC CACAGAAGCG CTGCTGAAGA CCGGAGCTGC TTACCCCTGT 
TTCTGCTCAC CCCAGCGGCT GGAGCTCCTG AAGAAGGAGG CCTTGCGGAA CCACCAGACG 
CCCCGGTATG ACAATCGGTG CAGGAACATG AGCCAGGAGC AGGTGGCCCA GAAGCTGGCC 
AAGGACCCCA AGCCTGCGAT CCGCTTCCGC CTGGAGCAGG TGGTGCCAGC CTTCCAGGAC 
CTGGTCTATG GCTGGAATAG GCATGAAGTG GCCAGCGTGG AGGGAGACCC AGTCATCATG 
AAGAGCGACG GCTTCCCCAC ATACCACCTG GCCTGCGTGG TGGACGACCA CCACATGGGC 
ATCAGCCACG TGCTGCGAGG CTCTGAGTGG CTCGTCTCCA CTGCCAAGCA CCTGCTCCTC 
TACCAGGCCC TGGGCTGGCA GCCACCCCAC TTCGCCCACC TGCCCCTGCT CCTCAACAGG 
GATGGCAGCA AGCTCTCCAA GAGGCAAGGG GACGTTTTCC TGGAGCACTT TGCTGCTGAT 
GGCTTCCTGC CCGATTCCTT GTTGGACATC ATCACCAACT GTGGCTCAGG TTTTGCAGAG 
AACCAAATGG GCAGGACCCT GCCGGAGCTG ATCACACAGT TCAACCTGAC ACAGGTCACC 
TGTCACTCAG CCCTGCTGGA CCTGGAGAAG CTCCCAGAAT TCAACAGACT GCACCTCCAG 
CGGCTGGTGA GCAATGAGAG CCAGAGGCGC CAGCTGGTGG GGAAGCTGCA GGTCCTTGTG 
GAGGAGGCCT TTGGTTGCCA GCTGCAAAAC AGGGATGTCC TCAACCCAGT CTACGTGGAG 
AGGATCCTCC TGCTGAGACA GGGTCACATT TGCCGCCTGC AGGACTTGGT GTCCCCAGTA 
TACTCTTACC TGTGGACTCG CCCTGCAGTA GGTCGAGCAC AGCTGGACGC CATCTCGGAG 
AAGGTGGATG TGATTGCCAA GCGTGTGCTG GGGCTTCTAG AAAGATCTAG TATGAGCTTA 
ACTCAGGATA TGCTGAATGG AGAACTGAAG AAGCTATCAG AAGGTCTGGA AGGCACCAAG 
TACAGTAATG TGATGAAACT CCTTCGGATG GCCCTCAGTG GACAGCAGGT GAGGCAGGGA 
CACGGGTTGG ATTGTTCCCT GGAGCCCCTC ATTGATCCTT TGAACTTACA TTTCCTGGCA 
GGCACTGAGC TCAATATTGA GTATACAAAG GTGAATGAAA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295140.1
CDS	33..1637
Translation	MAALLRRLLQRERPSAASGRPVGRREANLGTDAGVAVRVRFAPSPTGFLHLGGLRTALYNYIFAKKYQGSFILRLEDTDQTRVVPGAAENIEDMLEWAGIPPDESPRRGGPAGPYQQSQRLELYAQATEALLKTGAAYPCFCSPQRLELLKKEALRNHQTPRYDNRCRNMSQEQVAQKLAKDPKPAIRFRLEQVVPAFQDLVYGWNRHEVASVEGDPVIMKSDGFPTYHLACVVDDHHMGISHVLRGSEWLVSTAKHLLLYQALGWQPPHFAHLPLLLNRDGSKLSKRQGDVFLEHFAADGFLPDSLLDIITNCGSGFAENQMGRTLPELITQFNLTQVTCHSALLDLEKLPEFNRLHLQRLVSNESQRRQLVGKLQVLVEEAFGCQLQNRDVLNPVYVERILLLRQGHICRLQDLVSPVYSYLWTRPAVGRAQLDAISEKVDVIAKRVLGLLERSSMSLTQDMLNGELKKLSEGLEGTKYSNVMKLLRMALSGQQVRQGHGLDCSLEPLIDPLNLHFLAGTELNIEYTKVNET

Target ORF information:

RefSeq Version	NM_001308211.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308211.1

ORF Insert Sequence:

ATGGCGGCGC TCCTGAGGAG ACTGCTGCAG CGCGAGAGGC CTTCGGCGGC CTCTGGCCGC 
CCCGTAGGAC GGCGCGAGGC CAACCTGGGC ACTGATGCCG GGGTTGCGGT GCGAGTGCGG 
TTCGCTCCCA GCCCCACAGG CTTCTTGCAC CTGGGTGGCC TCCGCACTGC CTTGTACAAC 
TACATCTTTG CTAAGAAGTA CCAGGGGAGC TTCATCCTGA GGCTAGAGGA CACAGATCAG 
ACTCGCGTTG TGCCTGGGGC AGCGGAGAAT ATTGAGGACA TGCTGGAGTG GGCAGGCATC 
CCGCCTGATG AGAGCCCCCG CCGGGGCGGT CCTGCTGGGC CCTACCAGCA ATCTCAGCGG 
TTGGAGCTGT ATGCCCAGGC CACAGAAGCG CTGCTGAAGA CCGGAGCTGC TTACCCCTGT 
TTCTGCTCAC CCCAGCGGCT GGAGCTCCTG AAGAAGGAGG CCTTGCGGAA CCACCAGACG 
CCCCGGTATG ACAATCGGTG CAGGAACATG AGCCAGGAGC AGGTGGCCCA GAAGCTGGCC 
AAGGACCCCA AGCCTGCGAT CCGCTTCCGC CTGGAGCAGG TGGTGCCAGC CTTCCAGGAC 
CTGGTCTATG GCTGGAATAG GCATGAAGTG GCCAGCGTGG AGGGAGACCC AGTCATCATG 
AAGAGCGACG GCTTCCCCAC ATACCACCTG GCCTGCGTGG TGGACGACCA CCACATGGGC 
ATCAGCCACG TGCTGCGAGG CTCTGAGTGG CTCGTCTCCA CTGCCAAGCA CCTGCTCCTC 
TACCAGGCCC TGGGCTGGCA GCCACCCCAC TTCGCCCACC TGCCCCTGCT CCTCAACAGG 
GATGGCAGCA AGCTCTCCAA GAGGCAAGGG GACGTTTTCC TGGAGCACTT TGCTGCTGAT 
GGCTTCCTGC CCGATTCCTT GTTGGACATC ATCACCAACT GTGGCTCAGG TTTTGCAGAG 
AACCAAATGG GCAGGACCCT GCCGGAGCTG ATCACACAGT TCAACCTGAC ACAGGTCACC 
TGTCACTCAG CCCTGCTGGA CCTGGAGAAG CTCCCAGAAT TCAACAGACT GCACCTCCAG 
CGGCTGGTGA GCAATGAGAG CCAGAGGCGC CAGCTGGTGG GGAAGCTGCA GGTCCTTGTG 
GAGGAGGCCT TTGGTTGCCA GCTGCAAAAC AGGGATGTCC TCAACCCAGT CTACGTGGAG 
AGGATCCTCC TGCTGAGACA GGGTCACATT TGCCGCCTGC AGGACTTGGT GTCCCCAGTA 
TACTCTTACC TGTGGACTCG CCCTGCAGTA GGTCGAGCAC AGCTGGACGC CATCTCGGAG 
AAGGTGGATG TGATTGCCAA GCGTGTGCTG GGGCTTCTAG AAAGATCTAG TATGAGCTTA 
ACTCAGGATA TGCTGAATGG AGAACTGAAG AAGCTATCAG AAGGTCTGGA AGGCACCAAG 
TACAGTAATG TGATGAAACT CCTTCGGATG GCCCTCAGTG GACAGCAGGT GAGGCAGGGA 
CACGGGTTGG ATTGTTCCCT GGAGCCCCTC ATTGATCCTT TGAACTTACA TTTCCTGGCA 
GGCACTGAGC TCAATATTGA GTATACAAAG GTGAATGAAA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EARS2 ( NM_001083614.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077083.1 Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25880
Clone ID Related Accession (Same CDS sequence)	NM_001083614.1 , NM_001083614.2
Accession Version	NM_001083614.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	probable glutamate--tRNA ligase, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA692403.1, AB075850.1, AC002400.1 and CA306283.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.74198.1, SRR1660805.73000.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC TCCTGAGGAG ACTGCTGCAG CGCGAGAGGC CTTCGGCGGC CTCTGGCCGC 
CCCGTAGGAC GGCGCGAGGC CAACCTGGGC ACTGATGCCG GGGTTGCGGT GCGAGTGCGG 
TTCGCTCCCA GCCCCACAGG CTTCTTGCAC CTGGGTGGCC TCCGCACTGC CTTGTACAAC 
TACATCTTTG CTAAGAAGTA CCAGGGGAGC TTCATCCTGA GGCTAGAGGA CACAGATCAG 
ACTCGCGTTG TGCCTGGGGC AGCGGAGAAT ATTGAGGACA TGCTGGAGTG GGCAGGCATC 
CCGCCTGATG AGAGCCCCCG CCGGGGCGGT CCTGCTGGGC CCTACCAGCA ATCTCAGCGG 
TTGGAGCTGT ATGCCCAGGC CACAGAAGCG CTGCTGAAGA CCGGAGCTGC TTACCCCTGT 
TTCTGCTCAC CCCAGCGGCT GGAGCTCCTG AAGAAGGAGG CCTTGCGGAA CCACCAGACG 
CCCCGGTATG ACAATCGGTG CAGGAACATG AGCCAGGAGC AGGTGGCCCA GAAGCTGGCC 
AAGGACCCCA AGCCTGCGAT CCGCTTCCGC CTGGAGCAGG TGGTGCCAGC CTTCCAGGAC 
CTGGTCTATG GCTGGAATAG GCATGAAGTG GCCAGCGTGG AGGGAGACCC AGTCATCATG 
AAGAGCGACG GCTTCCCCAC ATACCACCTG GCCTGCGTGG TGGACGACCA CCACATGGGC 
ATCAGCCACG TGCTGCGAGG CTCTGAGTGG CTCGTCTCCA CTGCCAAGCA CCTGCTCCTC 
TACCAGGCCC TGGGCTGGCA GCCACCCCAC TTCGCCCACC TGCCCCTGCT CCTCAACAGG 
GATGGCAGCA AGCTCTCCAA GAGGCAAGGG GACGTTTTCC TGGAGCACTT TGCTGCTGAT 
GGCTTCCTGC CCGATTCCTT GTTGGACATC ATCACCAACT GTGGCTCAGG TTTTGCAGAG 
AACCAAATGG GCAGGACCCT GCCGGAGCTG ATCACACAGT TCAACCTGAC ACAGGTCACC 
TGTCACTCAG CCCTGCTGGA CCTGGAGAAG CTCCCAGAAT TCAACAGACT GCACCTCCAG 
CGGCTGGTGA GCAATGAGAG CCAGAGGCGC CAGCTGGTGG GGAAGCTGCA GGTCCTTGTG 
GAGGAGGCCT TTGGTTGCCA GCTGCAAAAC AGGGATGTCC TCAACCCAGT CTACGTGGAG 
AGGATCCTCC TGCTGAGACA GGGTCACATT TGCCGCCTGC AGGACTTGGT GTCCCCAGTA 
TACTCTTACC TGTGGACTCG CCCTGCAGTA GGTCGAGCAC AGCTGGACGC CATCTCGGAG 
AAGGTGGATG TGATTGCCAA GCGTGTGCTG GGGCTTCTAG AAAGATCTAG TATGAGCTTA 
ACTCAGGATA TGCTGAATGG AGAACTGAAG AAGCTATCAG AAGGTCTGGA AGGCACCAAG 
TACAGTAATG TGATGAAACT CCTTCGGATG GCCCTCAGTG GACAGCAGCA AGGACCTCCT 
GTAGCTGAGA TGATGTTGGC CTTGGGACCA AAGGAAGTAC GGGAACGGAT CCAGAAGGTG 
GTTTCCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077083.1
CDS	33..1604
Translation	MAALLRRLLQRERPSAASGRPVGRREANLGTDAGVAVRVRFAPSPTGFLHLGGLRTALYNYIFAKKYQGSFILRLEDTDQTRVVPGAAENIEDMLEWAGIPPDESPRRGGPAGPYQQSQRLELYAQATEALLKTGAAYPCFCSPQRLELLKKEALRNHQTPRYDNRCRNMSQEQVAQKLAKDPKPAIRFRLEQVVPAFQDLVYGWNRHEVASVEGDPVIMKSDGFPTYHLACVVDDHHMGISHVLRGSEWLVSTAKHLLLYQALGWQPPHFAHLPLLLNRDGSKLSKRQGDVFLEHFAADGFLPDSLLDIITNCGSGFAENQMGRTLPELITQFNLTQVTCHSALLDLEKLPEFNRLHLQRLVSNESQRRQLVGKLQVLVEEAFGCQLQNRDVLNPVYVERILLLRQGHICRLQDLVSPVYSYLWTRPAVGRAQLDAISEKVDVIAKRVLGLLERSSMSLTQDMLNGELKKLSEGLEGTKYSNVMKLLRMALSGQQQGPPVAEMMLALGPKEVRERIQKVVSS

Target ORF information:

RefSeq Version	NM_001083614.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083614.1

ORF Insert Sequence:

ATGGCGGCGC TCCTGAGGAG ACTGCTGCAG CGCGAGAGGC CTTCGGCGGC CTCTGGCCGC 
CCCGTAGGAC GGCGCGAGGC CAACCTGGGC ACTGATGCCG GGGTTGCGGT GCGAGTGCGG 
TTCGCTCCCA GCCCCACAGG CTTCTTGCAC CTGGGTGGCC TCCGCACTGC CTTGTACAAC 
TACATCTTTG CTAAGAAGTA CCAGGGGAGC TTCATCCTGA GGCTAGAGGA CACAGATCAG 
ACTCGCGTTG TGCCTGGGGC AGCGGAGAAT ATTGAGGACA TGCTGGAGTG GGCAGGCATC 
CCGCCTGATG AGAGCCCCCG CCGGGGCGGT CCTGCTGGGC CCTACCAGCA ATCTCAGCGG 
TTGGAGCTGT ATGCCCAGGC CACAGAAGCG CTGCTGAAGA CCGGAGCTGC TTACCCCTGT 
TTCTGCTCAC CCCAGCGGCT GGAGCTCCTG AAGAAGGAGG CCTTGCGGAA CCACCAGACG 
CCCCGGTATG ACAATCGGTG CAGGAACATG AGCCAGGAGC AGGTGGCCCA GAAGCTGGCC 
AAGGACCCCA AGCCTGCGAT CCGCTTCCGC CTGGAGCAGG TGGTGCCAGC CTTCCAGGAC 
CTGGTCTATG GCTGGAATAG GCATGAAGTG GCCAGCGTGG AGGGAGACCC AGTCATCATG 
AAGAGCGACG GCTTCCCCAC ATACCACCTG GCCTGCGTGG TGGACGACCA CCACATGGGC 
ATCAGCCACG TGCTGCGAGG CTCTGAGTGG CTCGTCTCCA CTGCCAAGCA CCTGCTCCTC 
TACCAGGCCC TGGGCTGGCA GCCACCCCAC TTCGCCCACC TGCCCCTGCT CCTCAACAGG 
GATGGCAGCA AGCTCTCCAA GAGGCAAGGG GACGTTTTCC TGGAGCACTT TGCTGCTGAT 
GGCTTCCTGC CCGATTCCTT GTTGGACATC ATCACCAACT GTGGCTCAGG TTTTGCAGAG 
AACCAAATGG GCAGGACCCT GCCGGAGCTG ATCACACAGT TCAACCTGAC ACAGGTCACC 
TGTCACTCAG CCCTGCTGGA CCTGGAGAAG CTCCCAGAAT TCAACAGACT GCACCTCCAG 
CGGCTGGTGA GCAATGAGAG CCAGAGGCGC CAGCTGGTGG GGAAGCTGCA GGTCCTTGTG 
GAGGAGGCCT TTGGTTGCCA GCTGCAAAAC AGGGATGTCC TCAACCCAGT CTACGTGGAG 
AGGATCCTCC TGCTGAGACA GGGTCACATT TGCCGCCTGC AGGACTTGGT GTCCCCAGTA 
TACTCTTACC TGTGGACTCG CCCTGCAGTA GGTCGAGCAC AGCTGGACGC CATCTCGGAG 
AAGGTGGATG TGATTGCCAA GCGTGTGCTG GGGCTTCTAG AAAGATCTAG TATGAGCTTA 
ACTCAGGATA TGCTGAATGG AGAACTGAAG AAGCTATCAG AAGGTCTGGA AGGCACCAAG 
TACAGTAATG TGATGAAACT CCTTCGGATG GCCCTCAGTG GACAGCAGCA AGGACCTCCT 
GTAGCTGAGA TGATGTTGGC CTTGGGACCA AAGGAAGTAC GGGAACGGAT CCAGAAGGTG 
GTTTCCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EARS2 ( NM_001083614.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001077083.1 Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25880
Clone ID Related Accession (Same CDS sequence)	NM_001083614.1 , NM_001083614.2
Accession Version	NM_001083614.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	probable glutamate--tRNA ligase, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002400.1. This sequence is a reference standard in the RefSeqGene project. On Jul 1, 2019 this sequence version replaced NM_001083614.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGC TCCTGAGGAG ACTGCTGCAG CGCGAGAGGC CTTCGGCGGC CTCTGGCCGC 
CCCGTAGGAC GGCGCGAGGC CAACCTGGGC ACTGATGCCG GGGTTGCGGT GCGAGTGCGG 
TTCGCTCCCA GCCCCACAGG CTTCTTGCAC CTGGGTGGCC TCCGCACTGC CTTGTACAAC 
TACATCTTTG CTAAGAAGTA CCAGGGGAGC TTCATCCTGA GGCTAGAGGA CACAGATCAG 
ACTCGCGTTG TGCCTGGGGC AGCGGAGAAT ATTGAGGACA TGCTGGAGTG GGCAGGCATC 
CCGCCTGATG AGAGCCCCCG CCGGGGCGGT CCTGCTGGGC CCTACCAGCA ATCTCAGCGG 
TTGGAGCTGT ATGCCCAGGC CACAGAAGCG CTGCTGAAGA CCGGAGCTGC TTACCCCTGT 
TTCTGCTCAC CCCAGCGGCT GGAGCTCCTG AAGAAGGAGG CCTTGCGGAA CCACCAGACG 
CCCCGGTATG ACAATCGGTG CAGGAACATG AGCCAGGAGC AGGTGGCCCA GAAGCTGGCC 
AAGGACCCCA AGCCTGCGAT CCGCTTCCGC CTGGAGCAGG TGGTGCCAGC CTTCCAGGAC 
CTGGTCTATG GCTGGAATAG GCATGAAGTG GCCAGCGTGG AGGGAGACCC AGTCATCATG 
AAGAGCGACG GCTTCCCCAC ATACCACCTG GCCTGCGTGG TGGACGACCA CCACATGGGC 
ATCAGCCACG TGCTGCGAGG CTCTGAGTGG CTCGTCTCCA CTGCCAAGCA CCTGCTCCTC 
TACCAGGCCC TGGGCTGGCA GCCACCCCAC TTCGCCCACC TGCCCCTGCT CCTCAACAGG 
GATGGCAGCA AGCTCTCCAA GAGGCAAGGG GACGTTTTCC TGGAGCACTT TGCTGCTGAT 
GGCTTCCTGC CCGATTCCTT GTTGGACATC ATCACCAACT GTGGCTCAGG TTTTGCAGAG 
AACCAAATGG GCAGGACCCT GCCGGAGCTG ATCACACAGT TCAACCTGAC ACAGGTCACC 
TGTCACTCAG CCCTGCTGGA CCTGGAGAAG CTCCCAGAAT TCAACAGACT GCACCTCCAG 
CGGCTGGTGA GCAATGAGAG CCAGAGGCGC CAGCTGGTGG GGAAGCTGCA GGTCCTTGTG 
GAGGAGGCCT TTGGTTGCCA GCTGCAAAAC AGGGATGTCC TCAACCCAGT CTACGTGGAG 
AGGATCCTCC TGCTGAGACA GGGTCACATT TGCCGCCTGC AGGACTTGGT GTCCCCAGTA 
TACTCTTACC TGTGGACTCG CCCTGCAGTA GGTCGAGCAC AGCTGGACGC CATCTCGGAG 
AAGGTGGATG TGATTGCCAA GCGTGTGCTG GGGCTTCTAG AAAGATCTAG TATGAGCTTA 
ACTCAGGATA TGCTGAATGG AGAACTGAAG AAGCTATCAG AAGGTCTGGA AGGCACCAAG 
TACAGTAATG TGATGAAACT CCTTCGGATG GCCCTCAGTG GACAGCAGCA AGGACCTCCT 
GTAGCTGAGA TGATGTTGGC CTTGGGACCA AAGGAAGTAC GGGAACGGAT CCAGAAGGTG 
GTTTCCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077083.1
CDS	8..1579
Translation	MAALLRRLLQRERPSAASGRPVGRREANLGTDAGVAVRVRFAPSPTGFLHLGGLRTALYNYIFAKKYQGSFILRLEDTDQTRVVPGAAENIEDMLEWAGIPPDESPRRGGPAGPYQQSQRLELYAQATEALLKTGAAYPCFCSPQRLELLKKEALRNHQTPRYDNRCRNMSQEQVAQKLAKDPKPAIRFRLEQVVPAFQDLVYGWNRHEVASVEGDPVIMKSDGFPTYHLACVVDDHHMGISHVLRGSEWLVSTAKHLLLYQALGWQPPHFAHLPLLLNRDGSKLSKRQGDVFLEHFAADGFLPDSLLDIITNCGSGFAENQMGRTLPELITQFNLTQVTCHSALLDLEKLPEFNRLHLQRLVSNESQRRQLVGKLQVLVEEAFGCQLQNRDVLNPVYVERILLLRQGHICRLQDLVSPVYSYLWTRPAVGRAQLDAISEKVDVIAKRVLGLLERSSMSLTQDMLNGELKKLSEGLEGTKYSNVMKLLRMALSGQQQGPPVAEMMLALGPKEVRERIQKVVSS

Target ORF information:

RefSeq Version	NM_001083614.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083614.2

ORF Insert Sequence:

ATGGCGGCGC TCCTGAGGAG ACTGCTGCAG CGCGAGAGGC CTTCGGCGGC CTCTGGCCGC 
CCCGTAGGAC GGCGCGAGGC CAACCTGGGC ACTGATGCCG GGGTTGCGGT GCGAGTGCGG 
TTCGCTCCCA GCCCCACAGG CTTCTTGCAC CTGGGTGGCC TCCGCACTGC CTTGTACAAC 
TACATCTTTG CTAAGAAGTA CCAGGGGAGC TTCATCCTGA GGCTAGAGGA CACAGATCAG 
ACTCGCGTTG TGCCTGGGGC AGCGGAGAAT ATTGAGGACA TGCTGGAGTG GGCAGGCATC 
CCGCCTGATG AGAGCCCCCG CCGGGGCGGT CCTGCTGGGC CCTACCAGCA ATCTCAGCGG 
TTGGAGCTGT ATGCCCAGGC CACAGAAGCG CTGCTGAAGA CCGGAGCTGC TTACCCCTGT 
TTCTGCTCAC CCCAGCGGCT GGAGCTCCTG AAGAAGGAGG CCTTGCGGAA CCACCAGACG 
CCCCGGTATG ACAATCGGTG CAGGAACATG AGCCAGGAGC AGGTGGCCCA GAAGCTGGCC 
AAGGACCCCA AGCCTGCGAT CCGCTTCCGC CTGGAGCAGG TGGTGCCAGC CTTCCAGGAC 
CTGGTCTATG GCTGGAATAG GCATGAAGTG GCCAGCGTGG AGGGAGACCC AGTCATCATG 
AAGAGCGACG GCTTCCCCAC ATACCACCTG GCCTGCGTGG TGGACGACCA CCACATGGGC 
ATCAGCCACG TGCTGCGAGG CTCTGAGTGG CTCGTCTCCA CTGCCAAGCA CCTGCTCCTC 
TACCAGGCCC TGGGCTGGCA GCCACCCCAC TTCGCCCACC TGCCCCTGCT CCTCAACAGG 
GATGGCAGCA AGCTCTCCAA GAGGCAAGGG GACGTTTTCC TGGAGCACTT TGCTGCTGAT 
GGCTTCCTGC CCGATTCCTT GTTGGACATC ATCACCAACT GTGGCTCAGG TTTTGCAGAG 
AACCAAATGG GCAGGACCCT GCCGGAGCTG ATCACACAGT TCAACCTGAC ACAGGTCACC 
TGTCACTCAG CCCTGCTGGA CCTGGAGAAG CTCCCAGAAT TCAACAGACT GCACCTCCAG 
CGGCTGGTGA GCAATGAGAG CCAGAGGCGC CAGCTGGTGG GGAAGCTGCA GGTCCTTGTG 
GAGGAGGCCT TTGGTTGCCA GCTGCAAAAC AGGGATGTCC TCAACCCAGT CTACGTGGAG 
AGGATCCTCC TGCTGAGACA GGGTCACATT TGCCGCCTGC AGGACTTGGT GTCCCCAGTA 
TACTCTTACC TGTGGACTCG CCCTGCAGTA GGTCGAGCAC AGCTGGACGC CATCTCGGAG 
AAGGTGGATG TGATTGCCAA GCGTGTGCTG GGGCTTCTAG AAAGATCTAG TATGAGCTTA 
ACTCAGGATA TGCTGAATGG AGAACTGAAG AAGCTATCAG AAGGTCTGGA AGGCACCAAG 
TACAGTAATG TGATGAAACT CCTTCGGATG GCCCTCAGTG GACAGCAGCA AGGACCTCCT 
GTAGCTGAGA TGATGTTGGC CTTGGGACCA AAGGAAGTAC GGGAACGGAT CCAGAAGGTG 
GTTTCCAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EARS2 ( XM_011545738.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544040.1 Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant X1, mRNA.

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CloneID	OHu75297
Clone ID Related Accession (Same CDS sequence)	XM_011545738.1
Accession Version	XM_011545738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable glutamate--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCATTG ATGACAACCT GTGTAACAGG TGTTGTAGAC GCTGGGATAC AGTGATCATT 
GAGAAAGGCT TCTTGCACCT GGGTGGCCTC CGCACTGCCT TGTACAACTA CATCTTTGCT 
AAGAAGTACC AGGGGAGCTT CATCCTGAGG CTAGAGGACA CAGATCAGAC TCGCGTTGTG 
CCTGGGGCAG CGGAGAATAT TGAGGACATG CTGGAGTGGG CAGGCATCCC GCCTGATGAG 
AGCCCCCGCC GGGGCGGTCC TGCTGGGCCC TACCAGCAAT CTCAGCGGTT GGAGCTGTAT 
GCCCAGGCCA CAGAAGCGCT GCTGAAGACC GGAGCTGCTT ACCCCTGTTT CTGCTCACCC 
CAGCGGCTGG AGCTCCTGAA GAAGGAGGCC TTGCGGAACC ACCAGACGCC CCGGTATGAC 
AATCGGTGCA GGAACATGAG CCAGGAGCAG GTGGCCCAGA AGCTGGCCAA GGACCCCAAG 
CCTGCGATCC GCTTCCGCCT GGAGCAGGTG GTGCCAGCCT TCCAGGACCT GGTCTATGGC 
TGGAATAGGC ATGAAGTGGC CAGCGTGGAG GGAGACCCAG TCATCATGAA GAGCGACGGC 
TTCCCCACAT ACCACCTGGC CTGCGTGGTG GACGACCACC ACATGGGCAT CAGCCACGTG 
CTGCGAGGCT CTGAGTGGCT CGTCTCCACT GCCAAGCACC TGCTCCTCTA CCAGGCCCTG 
GGCTGGCAGC CACCCCACTT CGCCCACCTG CCCCTGCTCC TCAACAGGGA TGGCAGCAAG 
CTCTCCAAGA GGCAAGGGGA CGTTTTCCTG GAGCACTTTG CTGCTGATGG CTTCCTGCCC 
GATTCCTTGT TGGACATCAT CACCAACTGT GGCTCAGGTT TTGCAGAGAA CCAAATGGGC 
AGGACCCTGC CGGAGCTGAT CACACAGTTC AACCTGACAC AGGTCACCTG TCACTCAGCC 
CTGCTGGACC TGGAGAAGCT CCCAGAATTC AACAGACTGC ACCTCCAGCG GCTGGTGAGC 
AATGAGAGCC AGAGGCGCCA GCTGGTGGGG AAGCTGCAGG TCCTTGTGGA GGAGGCCTTT 
GGTTGCCAGC TGCAAAACAG GGATGTCCTC AACCCAGTCT ACGTGGAGAG GATCCTCCTG 
CTGAGACAGG GTCACATTTG CCGCCTGCAG GACTTGGTGT CCCCAGTATA CTCTTACCTG 
TGGACTCGCC CTGCAGTAGG TCGAGCACAG CTGGACGCCA TCTCGGAGAA GGTGGATGTG 
ATTGCCAAGC GTGTGCTGGG GCTTCTAGAA AGATCTAGTA TGAGCTTAAC TCAGGATATG 
CTGAATGGAG AACTGAAGAA GCTATCAGAA GGTCTGGAAG GCACCAAGTA CAGTAATGTG 
ATGAAACTCC TTCGGATGGC CCTCAGTGGA CAGCAGCAAG GACCTCCTGT AGCTGAGATG 
ATGTTGGCCT TGGGACCAAA GGAAGTACGG GAACGGATCC AGAAGGTGGT TTCCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544040.1
CDS	395..1894
Translation	MCIDDNLCNRCCRRWDTVIIEKGFLHLGGLRTALYNYIFAKKYQGSFILRLEDTDQTRVVPGAAENIEDMLEWAGIPPDESPRRGGPAGPYQQSQRLELYAQATEALLKTGAAYPCFCSPQRLELLKKEALRNHQTPRYDNRCRNMSQEQVAQKLAKDPKPAIRFRLEQVVPAFQDLVYGWNRHEVASVEGDPVIMKSDGFPTYHLACVVDDHHMGISHVLRGSEWLVSTAKHLLLYQALGWQPPHFAHLPLLLNRDGSKLSKRQGDVFLEHFAADGFLPDSLLDIITNCGSGFAENQMGRTLPELITQFNLTQVTCHSALLDLEKLPEFNRLHLQRLVSNESQRRQLVGKLQVLVEEAFGCQLQNRDVLNPVYVERILLLRQGHICRLQDLVSPVYSYLWTRPAVGRAQLDAISEKVDVIAKRVLGLLERSSMSLTQDMLNGELKKLSEGLEGTKYSNVMKLLRMALSGQQQGPPVAEMMLALGPKEVRERIQKVVSS

Target ORF information:

RefSeq Version	XM_011545738.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545738.1

ORF Insert Sequence:

ATGTGCATTG ATGACAACCT GTGTAACAGG TGTTGTAGAC GCTGGGATAC AGTGATCATT 
GAGAAAGGCT TCTTGCACCT GGGTGGCCTC CGCACTGCCT TGTACAACTA CATCTTTGCT 
AAGAAGTACC AGGGGAGCTT CATCCTGAGG CTAGAGGACA CAGATCAGAC TCGCGTTGTG 
CCTGGGGCAG CGGAGAATAT TGAGGACATG CTGGAGTGGG CAGGCATCCC GCCTGATGAG 
AGCCCCCGCC GGGGCGGTCC TGCTGGGCCC TACCAGCAAT CTCAGCGGTT GGAGCTGTAT 
GCCCAGGCCA CAGAAGCGCT GCTGAAGACC GGAGCTGCTT ACCCCTGTTT CTGCTCACCC 
CAGCGGCTGG AGCTCCTGAA GAAGGAGGCC TTGCGGAACC ACCAGACGCC CCGGTATGAC 
AATCGGTGCA GGAACATGAG CCAGGAGCAG GTGGCCCAGA AGCTGGCCAA GGACCCCAAG 
CCTGCGATCC GCTTCCGCCT GGAGCAGGTG GTGCCAGCCT TCCAGGACCT GGTCTATGGC 
TGGAATAGGC ATGAAGTGGC CAGCGTGGAG GGAGACCCAG TCATCATGAA GAGCGACGGC 
TTCCCCACAT ACCACCTGGC CTGCGTGGTG GACGACCACC ACATGGGCAT CAGCCACGTG 
CTGCGAGGCT CTGAGTGGCT CGTCTCCACT GCCAAGCACC TGCTCCTCTA CCAGGCCCTG 
GGCTGGCAGC CACCCCACTT CGCCCACCTG CCCCTGCTCC TCAACAGGGA TGGCAGCAAG 
CTCTCCAAGA GGCAAGGGGA CGTTTTCCTG GAGCACTTTG CTGCTGATGG CTTCCTGCCC 
GATTCCTTGT TGGACATCAT CACCAACTGT GGCTCAGGTT TTGCAGAGAA CCAAATGGGC 
AGGACCCTGC CGGAGCTGAT CACACAGTTC AACCTGACAC AGGTCACCTG TCACTCAGCC 
CTGCTGGACC TGGAGAAGCT CCCAGAATTC AACAGACTGC ACCTCCAGCG GCTGGTGAGC 
AATGAGAGCC AGAGGCGCCA GCTGGTGGGG AAGCTGCAGG TCCTTGTGGA GGAGGCCTTT 
GGTTGCCAGC TGCAAAACAG GGATGTCCTC AACCCAGTCT ACGTGGAGAG GATCCTCCTG 
CTGAGACAGG GTCACATTTG CCGCCTGCAG GACTTGGTGT CCCCAGTATA CTCTTACCTG 
TGGACTCGCC CTGCAGTAGG TCGAGCACAG CTGGACGCCA TCTCGGAGAA GGTGGATGTG 
ATTGCCAAGC GTGTGCTGGG GCTTCTAGAA AGATCTAGTA TGAGCTTAAC TCAGGATATG 
CTGAATGGAG AACTGAAGAA GCTATCAGAA GGTCTGGAAG GCACCAAGTA CAGTAATGTG 
ATGAAACTCC TTCGGATGGC CCTCAGTGGA CAGCAGCAAG GACCTCCTGT AGCTGAGATG 
ATGTTGGCCT TGGGACCAAA GGAAGTACGG GAACGGATCC AGAAGGTGGT TTCCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EARS2 ( XM_011545739.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544041.1 Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant X3, mRNA.

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CloneID	OHu75298
Clone ID Related Accession (Same CDS sequence)	XM_011545739.1
Accession Version	XM_011545739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable glutamate--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGT GGGCAGGCAT CCCGCCTGAT GAGAGCCCCC GCCGGGGCGG TCCTGCTGGG 
CCCTACCAGC AATCTCAGCG GTTGGAGCTG TATGCCCAGG CCACAGAAGC GCTGCTGAAG 
ACCGGAGCTG CTTACCCCTG TTTCTGCTCA CCCCAGCGGC TGGAGCTCCT GAAGAAGGAG 
GCCTTGCGGA ACCACCAGAC GCCCCGGTAT GACAATCGGT GCAGGAACAT GAGCCAGGAG 
CAGGTGGCCC AGAAGCTGGC CAAGGACCCC AAGCCTGCGA TCCGCTTCCG CCTGGAGCAG 
GTGGTGCCAG CCTTCCAGGA CCTGGTCTAT GGCTGGAATA GGCATGAAGT GGCCAGCGTG 
GAGGGAGACC CAGTCATCAT GAAGAGCGAC GGCTTCCCCA CATACCACCT GGCCTGCGTG 
GTGGACGACC ACCACATGGG CATCAGCCAC GTGCTGCGAG GCTCTGAGTG GCTCGTCTCC 
ACTGCCAAGC ACCTGCTCCT CTACCAGGCC CTGGGCTGGC AGCCACCCCA CTTCGCCCAC 
CTGCCCCTGC TCCTCAACAG GGATGGCAGC AAGCTCTCCA AGAGGCAAGG GGACGTTTTC 
CTGGAGCACT TTGCTGCTGA TGGCTTCCTG CCCGATTCCT TGTTGGACAT CATCACCAAC 
TGTGGCTCAG GTTTTGCAGA GAACCAAATG GGCAGGACCC TGCCGGAGCT GATCACACAG 
TTCAACCTGA CACAGGTCAC CTGTCACTCA GCCCTGCTGG ACCTGGAGAA GCTCCCAGAA 
TTCAACAGAC TGCACCTCCA GCGGCTGGTG AGCAATGAGA GCCAGAGGCG CCAGCTGGTG 
GGGAAGCTGC AGGTCCTTGT GGAGGAGGCC TTTGGTTGCC AGCTGCAAAA CAGGGATGTC 
CTCAACCCAG TCTACGTGGA GAGGATCCTC CTGCTGAGAC AGGGTCACAT TTGCCGCCTG 
CAGGACTTGG TGTCCCCAGT ATACTCTTAC CTGTGGACTC GCCCTGCAGT AGGTCGAGCA 
CAGCTGGACG CCATCTCGGA GAAGGTGGAT GTGATTGCCA AGCGTGTGCT GGGGCTTCTA 
GAAAGATCTA GTATGAGCTT AACTCAGGAT ATGCTGAATG GAGAACTGAA GAAGCTATCA 
GAAGGTCTGG AAGGCACCAA GTACAGTAAT GTGATGAAAC TCCTTCGGAT GGCCCTCAGT 
GGACAGCAGC AAGGACCTCC TGTAGCTGAG ATGATGTTGG CCTTGGGACC AAAGGAAGTA 
CGGGAACGGA TCCAGAAGGT GGTTTCCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544041.1
CDS	221..1513
Translation	MLEWAGIPPDESPRRGGPAGPYQQSQRLELYAQATEALLKTGAAYPCFCSPQRLELLKKEALRNHQTPRYDNRCRNMSQEQVAQKLAKDPKPAIRFRLEQVVPAFQDLVYGWNRHEVASVEGDPVIMKSDGFPTYHLACVVDDHHMGISHVLRGSEWLVSTAKHLLLYQALGWQPPHFAHLPLLLNRDGSKLSKRQGDVFLEHFAADGFLPDSLLDIITNCGSGFAENQMGRTLPELITQFNLTQVTCHSALLDLEKLPEFNRLHLQRLVSNESQRRQLVGKLQVLVEEAFGCQLQNRDVLNPVYVERILLLRQGHICRLQDLVSPVYSYLWTRPAVGRAQLDAISEKVDVIAKRVLGLLERSSMSLTQDMLNGELKKLSEGLEGTKYSNVMKLLRMALSGQQQGPPVAEMMLALGPKEVRERIQKVVSS

Target ORF information:

RefSeq Version	XM_011545739.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545739.1

ORF Insert Sequence:

ATGCTGGAGT GGGCAGGCAT CCCGCCTGAT GAGAGCCCCC GCCGGGGCGG TCCTGCTGGG 
CCCTACCAGC AATCTCAGCG GTTGGAGCTG TATGCCCAGG CCACAGAAGC GCTGCTGAAG 
ACCGGAGCTG CTTACCCCTG TTTCTGCTCA CCCCAGCGGC TGGAGCTCCT GAAGAAGGAG 
GCCTTGCGGA ACCACCAGAC GCCCCGGTAT GACAATCGGT GCAGGAACAT GAGCCAGGAG 
CAGGTGGCCC AGAAGCTGGC CAAGGACCCC AAGCCTGCGA TCCGCTTCCG CCTGGAGCAG 
GTGGTGCCAG CCTTCCAGGA CCTGGTCTAT GGCTGGAATA GGCATGAAGT GGCCAGCGTG 
GAGGGAGACC CAGTCATCAT GAAGAGCGAC GGCTTCCCCA CATACCACCT GGCCTGCGTG 
GTGGACGACC ACCACATGGG CATCAGCCAC GTGCTGCGAG GCTCTGAGTG GCTCGTCTCC 
ACTGCCAAGC ACCTGCTCCT CTACCAGGCC CTGGGCTGGC AGCCACCCCA CTTCGCCCAC 
CTGCCCCTGC TCCTCAACAG GGATGGCAGC AAGCTCTCCA AGAGGCAAGG GGACGTTTTC 
CTGGAGCACT TTGCTGCTGA TGGCTTCCTG CCCGATTCCT TGTTGGACAT CATCACCAAC 
TGTGGCTCAG GTTTTGCAGA GAACCAAATG GGCAGGACCC TGCCGGAGCT GATCACACAG 
TTCAACCTGA CACAGGTCAC CTGTCACTCA GCCCTGCTGG ACCTGGAGAA GCTCCCAGAA 
TTCAACAGAC TGCACCTCCA GCGGCTGGTG AGCAATGAGA GCCAGAGGCG CCAGCTGGTG 
GGGAAGCTGC AGGTCCTTGT GGAGGAGGCC TTTGGTTGCC AGCTGCAAAA CAGGGATGTC 
CTCAACCCAG TCTACGTGGA GAGGATCCTC CTGCTGAGAC AGGGTCACAT TTGCCGCCTG 
CAGGACTTGG TGTCCCCAGT ATACTCTTAC CTGTGGACTC GCCCTGCAGT AGGTCGAGCA 
CAGCTGGACG CCATCTCGGA GAAGGTGGAT GTGATTGCCA AGCGTGTGCT GGGGCTTCTA 
GAAAGATCTA GTATGAGCTT AACTCAGGAT ATGCTGAATG GAGAACTGAA GAAGCTATCA 
GAAGGTCTGG AAGGCACCAA GTACAGTAAT GTGATGAAAC TCCTTCGGAT GGCCCTCAGT 
GGACAGCAGC AAGGACCTCC TGTAGCTGAG ATGATGTTGG CCTTGGGACC AAAGGAAGTA 
CGGGAACGGA TCCAGAAGGT GGTTTCCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Multisystem fatal infantile disease caused by a novel homozygous EARS2 mutation.
Brain : a journal of neurology136(Pt 2)e228(2013 Feb)
Talim B,Pyle A,Griffin H,Topaloglu H,Tokatli A,Keogh MJ,Santibanez-Koref M,Chinnery PF,Horvath R

Leukoencephalopathy with thalamus and brainstem involvement and high lactate 'LTBL' caused by EARS2 mutations.
Brain : a journal of neurology135(Pt 5)1387-94(2012 May)
Steenweg ME,Ghezzi D,Haack T,Abbink TE,Martinelli D,van Berkel CG,Bley A,Diogo L,Grillo E,Te Water Naud? J,Strom TM,Bertini E,Prokisch H,van der Knaap MS,Zeviani M

Molecular diagnosis of infantile mitochondrial disease with targeted next-generation sequencing.
Science translational medicine4(118)118ra10(2012 Jan)
Calvo SE,Compton AG,Hershman SG,Lim SC,Lieber DS,Tucker EJ,Laskowski A,Garone C,Liu S,Jaffe DB,Christodoulou J,Fletcher JM,Bruno DL,Goldblatt J,Dimauro S,Thorburn DR,Mootha VK

Biogenesis of glutaminyl-mt tRNAGln in human mitochondria.
Proceedings of the National Academy of Sciences of the United States of America106(38)16209-14(2009 Sep)
Nagao A,Suzuki T,Katoh T,Sakaguchi Y,Suzuki T

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EARS2 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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