GPHB5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPHB5
Entrez Gene ID	122876
Full Name	glycoprotein hormone beta 5
Synonyms	B5,GPB5,ZLUT1
General protein information	Preferred Names glycoprotein hormone beta 5 Names glycoprotein hormone beta-5 glycoprotein beta 5 glycoprotein hormone beta subunit thyrostimulin subunit beta
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q23.2
Summary	GPHB5 is a cystine knot-forming polypeptide and a subunit of the dimeric glycoprotein hormone family (Hsu et al., 2002 [PubMed 12089349]).[supplied by OMIM, Mar 2008].
Disorder MIM:	609652

mRNA and Protein(s)

mRNA	Protein	Name
NM_145171.3	NP_660154.3	glycoprotein hormone beta-5 precursor
NM_145171.4	NP_660154.3	glycoprotein hormone beta-5 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GPHB5	XP_547848.1
Pan troglodytes (chimpanzee)	GPHB5	XP_003314412.1
Bos taurus (cattle)	GPHB5	XP_002691074.1
Gallus gallus (chicken)	LOC428919	XP_003641489.1
Danio rerio (zebrafish)	gphb5	NP_001159810.1
	GPHB5	XP_001100410.1
Rattus norvegicus (Norway rat)	Gphb5	NP_001007014.1
Homo sapiens (human)	GPHB5	NP_660154.3
Mus musculus (house mouse)	Gphb5	NP_783575.2
Xenopus tropicalis (tropical clawed frog)	gphb5	XP_002939523.1

Gene Ontology
Bibliography

Related articles in PubMed

Inflammatory cytokines regulate glycoprotein subunit beta5 of thyrostimulin through nuclear factor-kappaB.
Suzuki C, Nagasaki H, Okajima Y, Suga H, Ozaki N, Arima H, Iwasaki Y, Oiso Y
Endocrinology150(5)2237-43(2009 May)

Biochemical roles of the oligosaccharide chains in thyrostimulin, a heterodimeric hormone of glycoprotein hormone subunits alpha 2 (GPA2) and beta 5 (GPB5).
Okajima Y, Nagasaki H, Suzuki C, Suga H, Ozaki N, Arima H, Hamada Y, Civelli O, Oiso Y
Regulatory peptides148(1-3)62-7(2008 Jun)

A glycoprotein hormone expressed in corticotrophs exhibits unique binding properties on thyroid-stimulating hormone receptor.
Okada SL, Ellsworth JL, Durnam DM, Haugen HS, Holloway JL, Kelley ML, Lewis KE, Ren H, Sheppard PO, Storey HM, Waggie KS, Wolf AC, Yao LY, Webster PJ
Molecular endocrinology (Baltimore, Md.)20(2)414-25(2006 Feb)

Heterodimeric fly glycoprotein hormone-alpha2 (GPA2) and glycoprotein hormone-beta5 (GPB5) activate fly leucine-rich repeat-containing G protein-coupled receptor-1 (DLGR1) and stimulation of human thyrotropin receptors by chimeric fly GPA2 and human GPB5.
Sudo S, Kuwabara Y, Park JI, Hsu SY, Hsueh AJ
Endocrinology146(8)3596-604(2005 Aug)

The DNA sequence and analysis of human chromosome 14.
Heilig R, Eckenberg R, Petit JL, Fonknechten N, Da Silva C, Cattolico L, Levy M, Barbe V, de Berardinis V, Ureta-Vidal A, Pelletier E, Vico V, Anthouard V, Rowen L, Madan A, Qin S, Sun H, Du H, Pepin K, Artiguenave F, Robert C, Cruaud C, Br?ls T, Jaillon O, Friedlander L, Samson G, Brottier P, Cure S, S?gurens B, Ani?re F, Samain S, Crespeau H, Abbasi N, Aiach N, Boscus D, Dickhoff R, Dors M, Dubois I, Friedman C, Gouyvenoux M, James R, Madan A, Mairey-Estrada B, Mangenot S, Martins N, M?nard M, Oztas S, Ratcliffe A, Shaffer T, Trask B, Vacherie B, Bellemere C, Belser C, Besnard-Gonnet M, Bartol-Mavel D, Boutard M, Briez-Silla S, Combette S, Dufoss?-Laurent V, Ferron C, Lechaplais C, Louesse C, Muselet D, Magdelenat G, Pateau E, Petit E, Sirvain-Trukniewicz P, Trybou A, Vega-Czarny N, Bataille E, Bluet E, Bordelais I, Dubois M, Dumont C, Gu?rin T, Haffray S, Hammadi R, Muanga J, Pellouin V, Robert D, Wunderle E, Gauguet G, Roy A, Sainte-Marthe L, Verdier J, Verdier-Discala C, Hillier L, Fulton L, McPherson J, Matsuda F, Wilson R, Scarpelli C, Gyapay G, Wincker P, Saurin W, Qu?tier F, Waterston R, Hood L, Weissenbach J
Nature421(6923)601-7(2003 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inflammatory cytokines positively regulate thyrostimulin through NF-kappaB activation.
Title: Inflammatory cytokines regulate glycoprotein subunit beta5 of thyrostimulin through nuclear factor-kappaB.

Dual site-disrupted GPA2 and the GPB5 mutant were not expressed in either the conditioned medium or cell lysate.
Title: Biochemical roles of the oligosaccharide chains in thyrostimulin, a heterodimeric hormone of glycoprotein hormone subunits alpha 2 (GPA2) and beta 5 (GPB5).

Srosophila GPA2/GPB5 heterodimers did not activate human TSH or gonadotropin receptors, chimeric fly GPA2/human GPB5 heterodimers stimulated human TSH receptors. (GPA2 in drosophila)
Title: Heterodimeric fly glycoprotein hormone-alpha2 (GPA2) and glycoprotein hormone-beta5 (GPB5) activate fly leucine-rich repeat-containing G protein-coupled receptor-1 (DLGR1) and stimulation of human thyrotropin receptors by chimeric fly GPA2 and human GPB5.

identification and characterization of GPB5 genes
Title: Evolution of glycoprotein hormone subunit genes in bilateral metazoa: identification of two novel human glycoprotein hormone subunit family genes, GPA2 and GPB5.

Thyrostimulin, a heterodimer of two new human glycoprotein hormone subunits, activates the thyroid-stimulating hormone receptor (thyrostimulin)
Title: Thyrostimulin, a heterodimer of two new human glycoprotein hormone subunits, activates the thyroid-stimulating hormone receptor.

The following GPHB5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPHB5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55827	NM_145171.4 Latest version!	Homo sapiens glycoprotein hormone subunit beta 5 (GPHB5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu55827	NM_145171.3	Homo sapiens glycoprotein hormone beta 5 (GPHB5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPHB5 ( NM_145171.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_660154.3 Homo sapiens glycoprotein hormone beta 5 (GPHB5), mRNA.

Price & Availability↑

CloneID	OHu55827
Clone ID Related Accession (Same CDS sequence)	NM_145171.3 , NM_145171.4
Accession Version	NM_145171.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	glycoprotein hormone beta-5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM709443.1. On Sep 13, 2011 this sequence version replaced NM_145171.2. ##Evidence-Data-START## Transcript exon combination :: BM709443.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END##

ATGAAGCTGG CATTCCTCTT CCTTGGCCCC ATGGCCCTCC TCCTTCTGGC TGGCTATGGC 
TGTGTCCTCG GTGCCTCCAG TGGGAACCTG CGCACCTTTG TGGGCTGTGC CGTGAGGGAG 
TTTACTTTCC TGGCCAAGAA GCCAGGCTGC AGGGGCCTTC GGATCACCAC GGATGCCTGC 
TGGGGTCGCT GTGAGACCTG GGAGAAACCC ATTCTGGAAC CCCCCTATAT TGAAGCCCAT 
CATCGAGTCT GTACCTACAA CGAGACCAAA CAGGTGACTG TCAAGCTGCC CAACTGTGCC 
CCGGGAGTCG ACCCCTTCTA CACCTATCCC GTGGCCATCC GCTGTGACTG CGGAGCCTGC 
TCCACTGCCA CCACGGAGTG TGAGACCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660154.3
CDS	58..450
Translation	MKLAFLFLGPMALLLLAGYGCVLGASSGNLRTFVGCAVREFTFLAKKPGCRGLRITTDACWGRCETWEKPILEPPYIEAHHRVCTYNETKQVTVKLPNCAPGVDPFYTYPVAIRCDCGACSTATTECETI

Target ORF information:

RefSeq Version	NM_145171.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glycoprotein hormone beta 5 (GPHB5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145171.3

ORF Insert Sequence:

ATGAAGCTGG CATTCCTCTT CCTTGGCCCC ATGGCCCTCC TCCTTCTGGC TGGCTATGGC 
TGTGTCCTCG GTGCCTCCAG TGGGAACCTG CGCACCTTTG TGGGCTGTGC CGTGAGGGAG 
TTTACTTTCC TGGCCAAGAA GCCAGGCTGC AGGGGCCTTC GGATCACCAC GGATGCCTGC 
TGGGGTCGCT GTGAGACCTG GGAGAAACCC ATTCTGGAAC CCCCCTATAT TGAAGCCCAT 
CATCGAGTCT GTACCTACAA CGAGACCAAA CAGGTGACTG TCAAGCTGCC CAACTGTGCC 
CCGGGAGTCG ACCCCTTCTA CACCTATCCC GTGGCCATCC GCTGTGACTG CGGAGCCTGC 
TCCACTGCCA CCACGGAGTG TGAGACCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPHB5 ( NM_145171.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_660154.3 Homo sapiens glycoprotein hormone subunit beta 5 (GPHB5), mRNA.

Price & Availability↑

CloneID	OHu55827
Clone ID Related Accession (Same CDS sequence)	NM_145171.3 , NM_145171.4
Accession Version	NM_145171.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	glycoprotein hormone beta-5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL118555.6 and BM709443.1. On May 17, 2019 this sequence version replaced NM_145171.3. ##Evidence-Data-START## Transcript exon combination :: BM709443.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000621500.2/ ENSP00000478993.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGAAGCTGG CATTCCTCTT CCTTGGCCCC ATGGCCCTCC TCCTTCTGGC TGGCTATGGC 
TGTGTCCTCG GTGCCTCCAG TGGGAACCTG CGCACCTTTG TGGGCTGTGC CGTGAGGGAG 
TTTACTTTCC TGGCCAAGAA GCCAGGCTGC AGGGGCCTTC GGATCACCAC GGATGCCTGC 
TGGGGTCGCT GTGAGACCTG GGAGAAACCC ATTCTGGAAC CCCCCTATAT TGAAGCCCAT 
CATCGAGTCT GTACCTACAA CGAGACCAAA CAGGTGACTG TCAAGCTGCC CAACTGTGCC 
CCGGGAGTCG ACCCCTTCTA CACCTATCCC GTGGCCATCC GCTGTGACTG CGGAGCCTGC 
TCCACTGCCA CCACGGAGTG TGAGACCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660154.3
CDS	114..506
Translation	MKLAFLFLGPMALLLLAGYGCVLGASSGNLRTFVGCAVREFTFLAKKPGCRGLRITTDACWGRCETWEKPILEPPYIEAHHRVCTYNETKQVTVKLPNCAPGVDPFYTYPVAIRCDCGACSTATTECETI

Target ORF information:

RefSeq Version	NM_145171.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glycoprotein hormone subunit beta 5 (GPHB5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145171.4

ORF Insert Sequence:

ATGAAGCTGG CATTCCTCTT CCTTGGCCCC ATGGCCCTCC TCCTTCTGGC TGGCTATGGC 
TGTGTCCTCG GTGCCTCCAG TGGGAACCTG CGCACCTTTG TGGGCTGTGC CGTGAGGGAG 
TTTACTTTCC TGGCCAAGAA GCCAGGCTGC AGGGGCCTTC GGATCACCAC GGATGCCTGC 
TGGGGTCGCT GTGAGACCTG GGAGAAACCC ATTCTGGAAC CCCCCTATAT TGAAGCCCAT 
CATCGAGTCT GTACCTACAA CGAGACCAAA CAGGTGACTG TCAAGCTGCC CAACTGTGCC 
CCGGGAGTCG ACCCCTTCTA CACCTATCCC GTGGCCATCC GCTGTGACTG CGGAGCCTGC 
TCCACTGCCA CCACGGAGTG TGAGACCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inflammatory cytokines regulate glycoprotein subunit beta5 of thyrostimulin through nuclear factor-kappaB.
Endocrinology150(5)2237-43(2009 May)
Suzuki C,Nagasaki H,Okajima Y,Suga H,Ozaki N,Arima H,Iwasaki Y,Oiso Y

Biochemical roles of the oligosaccharide chains in thyrostimulin, a heterodimeric hormone of glycoprotein hormone subunits alpha 2 (GPA2) and beta 5 (GPB5).
Regulatory peptides148(1-3)62-7(2008 Jun)
Okajima Y,Nagasaki H,Suzuki C,Suga H,Ozaki N,Arima H,Hamada Y,Civelli O,Oiso Y

A glycoprotein hormone expressed in corticotrophs exhibits unique binding properties on thyroid-stimulating hormone receptor.
Molecular endocrinology (Baltimore, Md.)20(2)414-25(2006 Feb)
Okada SL,Ellsworth JL,Durnam DM,Haugen HS,Holloway JL,Kelley ML,Lewis KE,Ren H,Sheppard PO,Storey HM,Waggie KS,Wolf AC,Yao LY,Webster PJ

Heterodimeric fly glycoprotein hormone-alpha2 (GPA2) and glycoprotein hormone-beta5 (GPB5) activate fly leucine-rich repeat-containing G protein-coupled receptor-1 (DLGR1) and stimulation of human thyrotropin receptors by chimeric fly GPA2 and human GPB5.
Endocrinology146(8)3596-604(2005 Aug)
Sudo S,Kuwabara Y,Park JI,Hsu SY,Hsueh AJ

The DNA sequence and analysis of human chromosome 14.
Nature421(6923)601-7(2003 Feb)
Heilig R,Eckenberg R,Petit JL,Fonknechten N,Da Silva C,Cattolico L,Levy M,Barbe V,de Berardinis V,Ureta-Vidal A,Pelletier E,Vico V,Anthouard V,Rowen L,Madan A,Qin S,Sun H,Du H,Pepin K,Artiguenave F,Robert C,Cruaud C,Br?ls T,Jaillon O,Friedlander L,Samson G,Brottier P,Cure S,S?gurens B,Ani?re F,Samain S,Crespeau H,Abbasi N,Aiach N,Boscus D,Dickhoff R,Dors M,Dubois I,Friedman C,Gouyvenoux M,James R,Madan A,Mairey-Estrada B,Mangenot S,Martins N,M?nard M,Oztas S,Ratcliffe A,Shaffer T,Trask B,Vacherie B,Bellemere C,Belser C,Besnard-Gonnet M,Bartol-Mavel D,Boutard M,Briez-Silla S,Combette S,Dufoss?-Laurent V,Ferron C,Lechaplais C,Louesse C,Muselet D,Magdelenat G,Pateau E,Petit E,Sirvain-Trukniewicz P,Trybou A,Vega-Czarny N,Bataille E,Bluet E,Bordelais I,Dubois M,Dumont C,Gu?rin T,Haffray S,Hammadi R,Muanga J,Pellouin V,Robert D,Wunderle E,Gauguet G,Roy A,Sainte-Marthe L,Verdier J,Verdier-Discala C,Hillier L,Fulton L,McPherson J,Matsuda F,Wilson R,Scarpelli C,Gyapay G,Wincker P,Saurin W,Qu?tier F,Waterston R,Hood L,Weissenbach J

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GPHB5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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