NAA30 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NAA30
Entrez Gene ID	122830
Full Name	N(alpha)-acetyltransferase 30, NatC catalytic subunit
Synonyms	C14orf35,MAK3,Mak3p,NAT12,NAT12P
General protein information	Preferred Names N(alpha)-acetyltransferase 30, NatC catalytic subunit Names N-alpha-acetyltransferase 30 N-acetyltransferase 12 (GCN5-related, putative) N-acetyltransferase MAK3 homolog natC catalytic subunit putative N-acetyltransferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q22.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001011713.3	NP_001011713.2	N-alpha-acetyltransferase 30
NM_001011713.2	NP_001011713.2	N-alpha-acetyltransferase 30

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NAA30	NP_001192966.1
	NAA30	XP_001090355.1
Homo sapiens (human)	NAA30	NP_001011713.2
Pan troglodytes (chimpanzee)	NAA30	XP_001164011.1
Canis lupus familiaris (dog)	NAA30	XP_851927.1
Mus musculus (house mouse)	Naa30	NP_001074899.1
Xenopus tropicalis (tropical clawed frog)	naa30	XP_004917281.1
Rattus norvegicus (Norway rat)	Naa30	NP_001102569.1
Gallus gallus (chicken)	NAA30	XP_421440.4

Gene Ontology
Bibliography

Related articles in PubMed

Identification of an alternatively spliced nuclear isoform of human N-terminal acetyltransferase Naa30.
Varland S, Myklebust LM, Goks?yr S?, Glomnes N, Torsvik J, Varhaug JE, Arnesen T
Gene64427-37(2018 Feb)

Depletion of the human N-terminal acetyltransferase hNaa30 disrupts Golgi integrity and ARFRP1 localization.
Starheim KK, Kalvik TV, Bj?rk?y G, Arnesen T
Bioscience reports37(2)(2017 Apr)

A Role for Human N-alpha Acetyltransferase 30 (Naa30) in Maintaining Mitochondrial Integrity.
Van Damme P, Kalvik TV, Starheim KK, Jonckheere V, Myklebust LM, Menschaert G, Varhaug JE, Gevaert K, Arnesen T
Molecular & cellular proteomics : MCP15(11)3361-3372(2016 Nov)

Knockdown of NAT12/NAA30 reduces tumorigenic features of glioblastoma-initiating cells.
Mughal AA, Grieg Z, Skjellegrind H, Fayzullin A, Lamkhannat M, Joel M, Ahmed MS, Murrell W, Vik-Mo EO, Langmoen IA, Stangeland B
Molecular cancer14160(2015 Aug)

An organellar n?-acetyltransferase, naa60, acetylates cytosolic N termini of transmembrane proteins and maintains Golgi integrity.
Aksnes H, Van Damme P, Goris M, Starheim KK, Marie M, St?ve SI, Hoel C, Kalvik TV, Hole K, Glomnes N, Furnes C, Ljostveit S, Ziegler M, Niere M, Gevaert K, Arnesen T
Cell reports10(8)1362-74(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of full-length Naa30 increased cell viability.
Title: Identification of an alternatively spliced nuclear isoform of human N-terminal acetyltransferase Naa30.

ARFRP1 shifted from a predominantly cis-Golgi and endosome-to-trans-Golgi network localization to localizing both Golgi and non-Golgi vesicular structures in hNaa30-depleted cells.
Title: Depletion of the human N-terminal acetyltransferase hNaa30 disrupts Golgi integrity and ARFRP1 localization.

Data show that the human NatC complex Nt-acetylates a broad range of cellular proteins, and that depletion of the catalytic subunit Naa30 affects mitochondrial morphology and function.
Title: A Role for Human N-alpha Acetyltransferase 30 (Naa30) in Maintaining Mitochondrial Integrity.

NAT12/NAA30 plays an important role in growth and survival of GICs possibly by regulating hypoxia response (HIF1a), levels of p-MTOR (Ser2448) and the p53 pathway.
Title: Knockdown of NAT12/NAA30 reduces tumorigenic features of glioblastoma-initiating cells.

Human Naa30 (MAK3) is the catalytic subunit of the human NatC N-terminal acetyltransferase complex. NatC acetylates hydrophobic Met-starting N-termini, and NatC knockdown induces p53-dependent apoptosis.
Title: Knockdown of human N alpha-terminal acetyltransferase complex C leads to p53-dependent apoptosis and aberrant human Arl8b localization.

The following NAA30 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAA30 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31443	NM_001011713.3 Latest version!	Homo sapiens N-alpha-acetyltransferase 30, NatC catalytic subunit (NAA30), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu31443	NM_001011713.2	Homo sapiens N(alpha)-acetyltransferase 30, NatC catalytic subunit (NAA30), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAA30 ( NM_001011713.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001011713.2 Homo sapiens N-alpha-acetyltransferase 30, NatC catalytic subunit (NAA30), mRNA.

Price & Availability↑

CloneID	OHu31443
Clone ID Related Accession (Same CDS sequence)	NM_001011713.3 , NM_001011713.2
Accession Version	NM_001011713.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	N-alpha-acetyltransferase 30
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK092674.1, BC118589.1, BC048983.1 and AL355834.4. On Nov 22, 2018 this sequence version replaced NM_001011713.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.151325.1, SRR1660805.250676.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000556492.6/ ENSP00000452521.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGAGG TACCGCCTGG GCCTAGCAGC CTCCTCCCAC CACCAGCACC TCCGGCCCCG 
GCGGCGGTCG AGCCCCGCTG TCCCTTCCCG GCGGGGGCCG CCCTCGCCTG CTGCAGCGAG 
GACGAGGAGG ACGACGAAGA GCACGAAGGC GGCGGCAGCA GGAGCCCGGC GGGCGGAGAG 
TCGGCGACGG TGGCGGCCAA GGGGCATCCG TGCCTCCGCT GCCCTCAGCC GCCGCAGGAG 
CAGCAGCAGC TCAACGGATT GATTAGCCCC GAACTGCGGC ACCTCCGGGC GGCCGCCTCC 
CTCAAGAGCA AGGTCCTGAG CGTAGCAGAG GTGGCCGCGA CCACAGCCAC CCCTGACGGA 
GGCCCCAGAG CGACTGCAAC AAAAGGAGCC GGGGTACACT CGGGCGAGAG GCCCCCTCAC 
TCCCTCTCTA GTAATGCAAG AACTGCGGTC CCCAGCCCGG TGGAGGCAGC GGCGGCGAGC 
GATCCCGCGG CGGCCCGCAA TGGACTGGCC GAGGGCACCG AGCAGGAGGA GGAGGAGGAA 
GACGAGCAGG TGCGGCTGCT GTCTTCGTCC CTGACCGCCG ACTGCAGCTT AAGAAGCCCT 
TCGGGCAGGG AGGTTGAGCC TGGGGAGGAT CGGACGATAC GATATGTCCG ATATGAATCC 
GAGCTACAAA TGCCCGATAT CATGAGACTG ATCACCAAAG ATCTGTCCGA ACCCTACTCC 
ATTTATACCT ATAGATATTT TATCCACAAC TGGCCACAGC TGTGCTTCTT GGCCATGGTA 
GGGGAGGAGT GTGTAGGTGC CATCGTTTGC AAGTTGGATA TGCACAAAAA GATGTTCCGC 
AGAGGTTATA TAGCCATGTT AGCCGTGGAT TCCAAATACA GGAGAAATGG CATTGGTACT 
AACTTGGTTA AGAAAGCTAT ATATGCCATG GTTGAGGGAG ACTGTGATGA GGTTGTTTTG 
GAAACCGAAA TAACAAATAA GTCCGCTTTG AAACTTTATG AAAATCTTGG TTTTGTTCGA 
GATAAGAGGC TGTTCAGATA CTATTTAAAT GGAGTTGATG CACTGCGACT TAAACTGTGG 
CTGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011713.2
CDS	122..1210
Translation	MAEVPPGPSSLLPPPAPPAPAAVEPRCPFPAGAALACCSEDEEDDEEHEGGGSRSPAGGESATVAAKGHPCLRCPQPPQEQQQLNGLISPELRHLRAAASLKSKVLSVAEVAATTATPDGGPRATATKGAGVHSGERPPHSLSSNARTAVPSPVEAAAASDPAAARNGLAEGTEQEEEEEDEQVRLLSSSLTADCSLRSPSGREVEPGEDRTIRYVRYESELQMPDIMRLITKDLSEPYSIYTYRYFIHNWPQLCFLAMVGEECVGAIVCKLDMHKKMFRRGYIAMLAVDSKYRRNGIGTNLVKKAIYAMVEGDCDEVVLETEITNKSALKLYENLGFVRDKRLFRYYLNGVDALRLKLWLR

Target ORF information:

RefSeq Version	NM_001011713.3
Organism	Homo sapiens(human)
Definition	Homo sapiens N-alpha-acetyltransferase 30, NatC catalytic subunit (NAA30), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011713.3

ORF Insert Sequence:

ATGGCGGAGG TACCGCCTGG GCCTAGCAGC CTCCTCCCAC CACCAGCACC TCCGGCCCCG 
GCGGCGGTCG AGCCCCGCTG TCCCTTCCCG GCGGGGGCCG CCCTCGCCTG CTGCAGCGAG 
GACGAGGAGG ACGACGAAGA GCACGAAGGC GGCGGCAGCA GGAGCCCGGC GGGCGGAGAG 
TCGGCGACGG TGGCGGCCAA GGGGCATCCG TGCCTCCGCT GCCCTCAGCC GCCGCAGGAG 
CAGCAGCAGC TCAACGGATT GATTAGCCCC GAACTGCGGC ACCTCCGGGC GGCCGCCTCC 
CTCAAGAGCA AGGTCCTGAG CGTAGCAGAG GTGGCCGCGA CCACAGCCAC CCCTGACGGA 
GGCCCCAGAG CGACTGCAAC AAAAGGAGCC GGGGTACACT CGGGCGAGAG GCCCCCTCAC 
TCCCTCTCTA GTAATGCAAG AACTGCGGTC CCCAGCCCGG TGGAGGCAGC GGCGGCGAGC 
GATCCCGCGG CGGCCCGCAA TGGACTGGCC GAGGGCACCG AGCAGGAGGA GGAGGAGGAA 
GACGAGCAGG TGCGGCTGCT GTCTTCGTCC CTGACCGCCG ACTGCAGCTT AAGAAGCCCT 
TCGGGCAGGG AGGTTGAGCC TGGGGAGGAT CGGACGATAC GATATGTCCG ATATGAATCC 
GAGCTACAAA TGCCCGATAT CATGAGACTG ATCACCAAAG ATCTGTCCGA ACCCTACTCC 
ATTTATACCT ATAGATATTT TATCCACAAC TGGCCACAGC TGTGCTTCTT GGCCATGGTA 
GGGGAGGAGT GTGTAGGTGC CATCGTTTGC AAGTTGGATA TGCACAAAAA GATGTTCCGC 
AGAGGTTATA TAGCCATGTT AGCCGTGGAT TCCAAATACA GGAGAAATGG CATTGGTACT 
AACTTGGTTA AGAAAGCTAT ATATGCCATG GTTGAGGGAG ACTGTGATGA GGTTGTTTTG 
GAAACCGAAA TAACAAATAA GTCCGCTTTG AAACTTTATG AAAATCTTGG TTTTGTTCGA 
GATAAGAGGC TGTTCAGATA CTATTTAAAT GGAGTTGATG CACTGCGACT TAAACTGTGG 
CTGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAA30 ( NM_001011713.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001011713.2 Homo sapiens N(alpha)-acetyltransferase 30, NatC catalytic subunit (NAA30), mRNA.

Price & Availability↑

CloneID	OHu31443
Clone ID Related Accession (Same CDS sequence)	NM_001011713.3 , NM_001011713.2
Accession Version	NM_001011713.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	N-alpha-acetyltransferase 30
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK092674.1, BC118589.1 and BC048983.1. On Jun 13, 2008 this sequence version replaced NM_001011713.1. ##Evidence-Data-START## Transcript exon combination :: AK092674.1, SRR1660805.250676.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGG TACCGCCTGG GCCTAGCAGC CTCCTCCCAC CACCAGCACC TCCGGCCCCG 
GCGGCGGTCG AGCCCCGCTG TCCCTTCCCG GCGGGGGCCG CCCTCGCCTG CTGCAGCGAG 
GACGAGGAGG ACGACGAAGA GCACGAAGGC GGCGGCAGCA GGAGCCCGGC GGGCGGAGAG 
TCGGCGACGG TGGCGGCCAA GGGGCATCCG TGCCTCCGCT GCCCTCAGCC GCCGCAGGAG 
CAGCAGCAGC TCAACGGATT GATTAGCCCC GAACTGCGGC ACCTCCGGGC GGCCGCCTCC 
CTCAAGAGCA AGGTCCTGAG CGTAGCAGAG GTGGCCGCGA CCACAGCCAC CCCTGACGGA 
GGCCCCAGAG CGACTGCAAC AAAAGGAGCC GGGGTACACT CGGGCGAGAG GCCCCCTCAC 
TCCCTCTCTA GTAATGCAAG AACTGCGGTC CCCAGCCCGG TGGAGGCAGC GGCGGCGAGC 
GATCCCGCGG CGGCCCGCAA TGGACTGGCC GAGGGCACCG AGCAGGAGGA GGAGGAGGAA 
GACGAGCAGG TGCGGCTGCT GTCTTCGTCC CTGACCGCCG ACTGCAGCTT AAGAAGCCCT 
TCGGGCAGGG AGGTTGAGCC TGGGGAGGAT CGGACGATAC GATATGTCCG ATATGAATCC 
GAGCTACAAA TGCCCGATAT CATGAGACTG ATCACCAAAG ATCTGTCCGA ACCCTACTCC 
ATTTATACCT ATAGATATTT TATCCACAAC TGGCCACAGC TGTGCTTCTT GGCCATGGTA 
GGGGAGGAGT GTGTAGGTGC CATCGTTTGC AAGTTGGATA TGCACAAAAA GATGTTCCGC 
AGAGGTTATA TAGCCATGTT AGCCGTGGAT TCCAAATACA GGAGAAATGG CATTGGTACT 
AACTTGGTTA AGAAAGCTAT ATATGCCATG GTTGAGGGAG ACTGTGATGA GGTTGTTTTG 
GAAACCGAAA TAACAAATAA GTCCGCTTTG AAACTTTATG AAAATCTTGG TTTTGTTCGA 
GATAAGAGGC TGTTCAGATA CTATTTAAAT GGAGTTGATG CACTGCGACT TAAACTGTGG 
CTGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011713.2
CDS	155..1243
Translation	MAEVPPGPSSLLPPPAPPAPAAVEPRCPFPAGAALACCSEDEEDDEEHEGGGSRSPAGGESATVAAKGHPCLRCPQPPQEQQQLNGLISPELRHLRAAASLKSKVLSVAEVAATTATPDGGPRATATKGAGVHSGERPPHSLSSNARTAVPSPVEAAAASDPAAARNGLAEGTEQEEEEEDEQVRLLSSSLTADCSLRSPSGREVEPGEDRTIRYVRYESELQMPDIMRLITKDLSEPYSIYTYRYFIHNWPQLCFLAMVGEECVGAIVCKLDMHKKMFRRGYIAMLAVDSKYRRNGIGTNLVKKAIYAMVEGDCDEVVLETEITNKSALKLYENLGFVRDKRLFRYYLNGVDALRLKLWLR

Target ORF information:

RefSeq Version	NM_001011713.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N(alpha)-acetyltransferase 30, NatC catalytic subunit (NAA30), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011713.2

ORF Insert Sequence:

ATGGCGGAGG TACCGCCTGG GCCTAGCAGC CTCCTCCCAC CACCAGCACC TCCGGCCCCG 
GCGGCGGTCG AGCCCCGCTG TCCCTTCCCG GCGGGGGCCG CCCTCGCCTG CTGCAGCGAG 
GACGAGGAGG ACGACGAAGA GCACGAAGGC GGCGGCAGCA GGAGCCCGGC GGGCGGAGAG 
TCGGCGACGG TGGCGGCCAA GGGGCATCCG TGCCTCCGCT GCCCTCAGCC GCCGCAGGAG 
CAGCAGCAGC TCAACGGATT GATTAGCCCC GAACTGCGGC ACCTCCGGGC GGCCGCCTCC 
CTCAAGAGCA AGGTCCTGAG CGTAGCAGAG GTGGCCGCGA CCACAGCCAC CCCTGACGGA 
GGCCCCAGAG CGACTGCAAC AAAAGGAGCC GGGGTACACT CGGGCGAGAG GCCCCCTCAC 
TCCCTCTCTA GTAATGCAAG AACTGCGGTC CCCAGCCCGG TGGAGGCAGC GGCGGCGAGC 
GATCCCGCGG CGGCCCGCAA TGGACTGGCC GAGGGCACCG AGCAGGAGGA GGAGGAGGAA 
GACGAGCAGG TGCGGCTGCT GTCTTCGTCC CTGACCGCCG ACTGCAGCTT AAGAAGCCCT 
TCGGGCAGGG AGGTTGAGCC TGGGGAGGAT CGGACGATAC GATATGTCCG ATATGAATCC 
GAGCTACAAA TGCCCGATAT CATGAGACTG ATCACCAAAG ATCTGTCCGA ACCCTACTCC 
ATTTATACCT ATAGATATTT TATCCACAAC TGGCCACAGC TGTGCTTCTT GGCCATGGTA 
GGGGAGGAGT GTGTAGGTGC CATCGTTTGC AAGTTGGATA TGCACAAAAA GATGTTCCGC 
AGAGGTTATA TAGCCATGTT AGCCGTGGAT TCCAAATACA GGAGAAATGG CATTGGTACT 
AACTTGGTTA AGAAAGCTAT ATATGCCATG GTTGAGGGAG ACTGTGATGA GGTTGTTTTG 
GAAACCGAAA TAACAAATAA GTCCGCTTTG AAACTTTATG AAAATCTTGG TTTTGTTCGA 
GATAAGAGGC TGTTCAGATA CTATTTAAAT GGAGTTGATG CACTGCGACT TAAACTGTGG 
CTGCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of an alternatively spliced nuclear isoform of human N-terminal acetyltransferase Naa30.
Gene64427-37(2018 Feb)
Varland S,Myklebust LM,Goks?yr S?,Glomnes N,Torsvik J,Varhaug JE,Arnesen T

Depletion of the human N-terminal acetyltransferase hNaa30 disrupts Golgi integrity and ARFRP1 localization.
Bioscience reports37(2)(2017 Apr)
Starheim KK,Kalvik TV,Bj?rk?y G,Arnesen T

A Role for Human N-alpha Acetyltransferase 30 (Naa30) in Maintaining Mitochondrial Integrity.
Molecular & cellular proteomics : MCP15(11)3361-3372(2016 Nov)
Van Damme P,Kalvik TV,Starheim KK,Jonckheere V,Myklebust LM,Menschaert G,Varhaug JE,Gevaert K,Arnesen T

Knockdown of NAT12/NAA30 reduces tumorigenic features of glioblastoma-initiating cells.
Molecular cancer14160(2015 Aug)
Mughal AA,Grieg Z,Skjellegrind H,Fayzullin A,Lamkhannat M,Joel M,Ahmed MS,Murrell W,Vik-Mo EO,Langmoen IA,Stangeland B

An organellar n?-acetyltransferase, naa60, acetylates cytosolic N termini of transmembrane proteins and maintains Golgi integrity.
Cell reports10(8)1362-74(2015 Mar)
Aksnes H,Van Damme P,Goris M,Starheim KK,Marie M,St?ve SI,Hoel C,Kalvik TV,Hole K,Glomnes N,Furnes C,Ljostveit S,Ziegler M,Niere M,Gevaert K,Arnesen T

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NAA30 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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