CLIC2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLIC2
Entrez Gene ID	1193
Full Name	chloride intracellular channel 2
Synonyms	CLIC2b,MRXS32,XAP121
General protein information	Preferred Names chloride intracellular channel 2 Names chloride intracellular channel protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq28
Summary	This gene encodes a chloride intracellular channel protein. Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. This protein plays a role in inhibiting the function of ryanodine receptor 2. A mutation in this gene is the cause of an X-linked form of cognitive disability. [provided by RefSeq, Jul 2017].
Disorder MIM:	300138
Disorder Html:	?Mental retardation, X-linked, syndromic 32, 300886 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001289.6	NP_001280.3	chloride intracellular channel protein 2
NM_001289.5	NP_001280.3	chloride intracellular channel protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLIC2	XP_001144952.1
Gallus gallus (chicken)	CLIC2	NP_001026285.1
Oryza sativa (rice)	Os06g0232600	NP_001057228.1
	CLIC2	XP_001099159.1
Arabidopsis thaliana (thale cress)	DHAR3	NP_568336.1
Homo sapiens (human)	CLIC2	NP_001280.3
Canis lupus familiaris (dog)	CLIC2	NP_001182083.1
Bos taurus (cattle)	CLIC2	NP_001075196.1
Rattus norvegicus (Norway rat)	Clic2	NP_001009651.1
Danio rerio (zebrafish)	clic2	NP_001002561.1

Gene Ontology
Bibliography

Related articles in PubMed

Tilapia and human CLIC2 structures are highly conserved.
Zeng J, Li Z, Lui EY, Lam SH, Swaminathan K
Biochemical and biophysical research communications495(2)1752-1757(2018 Jan)

XLID-causing mutations and associated genes challenged in light of data from large-scale human exome sequencing.
Piton A, Redin C, Mandel JL
American journal of human genetics93(2)368-83(2013 Aug)

An X-linked channelopathy with cardiomegaly due to a CLIC2 mutation enhancing ryanodine receptor channel activity.
Takano K, Liu D, Tarpey P, Gallant E, Lam A, Witham S, Alexov E, Chaubey A, Stevenson RE, Schwartz CE, Board PG, Dulhunty AF
Human molecular genetics21(20)4497-507(2012 Oct)

A missense mutation in CLIC2 associated with intellectual disability is predicted by in silico modeling to affect protein stability and dynamics.
Witham S, Takano K, Schwartz C, Alexov E
Proteins79(8)2444-54(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a vital role for the CLIC2 protein in maintaining normal cognitive function via its interaction with RyRs in the brain.
Title: An X-linked channelopathy with cardiomegaly due to a CLIC2 mutation enhancing ryanodine receptor channel activity.

crystal structure of soluble human CICL2 and implications for function
Title: The crystal structure of human chloride intracellular channel protein 2: a disulfide bond with functional implications.

Human CLIC2 was crystallized in 2 different forms, in presence of GSSH. Form A displayed P2(1)2(1)2(1) symmetry, with unit-cell parameters a=44.0, b=74.7, c=79.8 A. Form B displayed P2(1) symmetry, with unit-cell parameters a=36.0, b=66.9, c=44.1 A.
Title: Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2).

CLIC2 forms pH-dependent chloride channels in vitro with higher channel activity at low pH levels and that the channels are subject to redox regulation
Title: Structure of the Janus protein human CLIC2.

CLIC2 inhibited cardiac ryanodine receptor Ca2+ release channels in lipid bilayers when added to the cytoplasmic side of the channels and inhibited Ca2+ release from cardiac sarcoplasmic reticulum vesicles
Title: CLIC-2 modulates cardiac ryanodine receptor Ca2+ release channels.

The following CLIC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLIC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20656	NM_001289.6 Latest version!	Homo sapiens chloride intracellular channel 2 (CLIC2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu20656	NM_001289.5	Homo sapiens chloride intracellular channel 2 (CLIC2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLIC2 ( NM_001289.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_001280.3 Homo sapiens chloride intracellular channel 2 (CLIC2), mRNA.

Price & Availability↑

CloneID	OHu20656
Clone ID Related Accession (Same CDS sequence)	NM_001289.6 , NM_001289.5
Accession Version	NM_001289.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	chloride intracellular channel protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292785.1, BC022305.1 and AC234781.7. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001289.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292785.1, SRR1660809.202711.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000369449.7/ ENSP00000358460.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCAGGCC TGCGGCCCGG CACTCAAGTG GACCCTGAGA TTGAGCTTTT TGTAAAGGCT 
GGAAGTGATG GAGAGAGTAT TGGAAACTGT CCCTTTTGCC AACGCCTTTT CATGATCCTC 
TGGCTTAAAG GAGTTAAATT TAATGTGACA ACTGTTGACA TGACCAGAAA GCCTGAAGAA 
CTAAAGGACT TAGCCCCAGG TACCAATCCT CCGTTCCTGG TGTATAACAA GGAGTTGAAA 
ACAGACTTCA TTAAAATTGA GGAGTTTTTA GAACAAACCC TGGCTCCTCC AAGGTACCCT 
CACCTGAGTC CCAAGTACAA GGAGTCTTTT GATGTGGGCT GTAACCTCTT TGCCAAGTTT 
TCTGCATACA TTAAGAATAC ACAAAAGGAG GCAAATAAGA ATTTTGAAAA ATCTCTGCTC 
AAAGAATTCA AGCGTCTGGA TGACTACTTA AACACCCCAC TTCTGGATGA AATTGATCCA 
GACAGTGCTG AGGAACCCCC AGTTTCCAGA AGACTATTCT TGGATGGGGA CCAGCTAACA 
CTGGCTGATT GTAGCTTGTT ACCCAAGCTG AACATTATTA AAGTTGCTGC CAAGAAATAT 
CGTGACTTTG ACATTCCAGC AGAATTCTCA GGAGTCTGGC GTTATCTCCA CAATGCCTAT 
GCCCGTGAAG AATTTACCCA CACGTGTCCT GAAGACAAAG AAATTGAAAA TACTTACGCA 
AATGTGGCTA AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280.3
CDS	188..931
Translation	MSGLRPGTQVDPEIELFVKAGSDGESIGNCPFCQRLFMILWLKGVKFNVTTVDMTRKPEELKDLAPGTNPPFLVYNKELKTDFIKIEEFLEQTLAPPRYPHLSPKYKESFDVGCNLFAKFSAYIKNTQKEANKNFEKSLLKEFKRLDDYLNTPLLDEIDPDSAEEPPVSRRLFLDGDQLTLADCSLLPKLNIIKVAAKKYRDFDIPAEFSGVWRYLHNAYAREEFTHTCPEDKEIENTYANVAKQKS

Target ORF information:

RefSeq Version	NM_001289.6
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride intracellular channel 2 (CLIC2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289.6

ORF Insert Sequence:

ATGTCAGGCC TGCGGCCCGG CACTCAAGTG GACCCTGAGA TTGAGCTTTT TGTAAAGGCT 
GGAAGTGATG GAGAGAGTAT TGGAAACTGT CCCTTTTGCC AACGCCTTTT CATGATCCTC 
TGGCTTAAAG GAGTTAAATT TAATGTGACA ACTGTTGACA TGACCAGAAA GCCTGAAGAA 
CTAAAGGACT TAGCCCCAGG TACCAATCCT CCGTTCCTGG TGTATAACAA GGAGTTGAAA 
ACAGACTTCA TTAAAATTGA GGAGTTTTTA GAACAAACCC TGGCTCCTCC AAGGTACCCT 
CACCTGAGTC CCAAGTACAA GGAGTCTTTT GATGTGGGCT GTAACCTCTT TGCCAAGTTT 
TCTGCATACA TTAAGAATAC ACAAAAGGAG GCAAATAAGA ATTTTGAAAA ATCTCTGCTC 
AAAGAATTCA AGCGTCTGGA TGACTACTTA AACACCCCAC TTCTGGATGA AATTGATCCA 
GACAGTGCTG AGGAACCCCC AGTTTCCAGA AGACTATTCT TGGATGGGGA CCAGCTAACA 
CTGGCTGATT GTAGCTTGTT ACCCAAGCTG AACATTATTA AAGTTGCTGC CAAGAAATAT 
CGTGACTTTG ACATTCCAGC AGAATTCTCA GGAGTCTGGC GTTATCTCCA CAATGCCTAT 
GCCCGTGAAG AATTTACCCA CACGTGTCCT GAAGACAAAG AAATTGAAAA TACTTACGCA 
AATGTGGCTA AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLIC2 ( NM_001289.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001280.3 Homo sapiens chloride intracellular channel 2 (CLIC2), mRNA.

Price & Availability↑

CloneID	OHu20656
Clone ID Related Accession (Same CDS sequence)	NM_001289.6 , NM_001289.5
Accession Version	NM_001289.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	chloride intracellular channel protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA901964.1, AK292785.1, BC022305.1 and AC234781.7. This sequence is a reference standard in the RefSeqGene project. On Aug 18, 2013 this sequence version replaced NM_001289.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.100853.1, SRR1803613.149627.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGGCC TGCGGCCCGG CACTCAAGTG GACCCTGAGA TTGAGCTTTT TGTAAAGGCT 
GGAAGTGATG GAGAGAGTAT TGGAAACTGT CCCTTTTGCC AACGCCTTTT CATGATCCTC 
TGGCTTAAAG GAGTTAAATT TAATGTGACA ACTGTTGACA TGACCAGAAA GCCTGAAGAA 
CTAAAGGACT TAGCCCCAGG TACCAATCCT CCGTTCCTGG TGTATAACAA GGAGTTGAAA 
ACAGACTTCA TTAAAATTGA GGAGTTTTTA GAACAAACCC TGGCTCCTCC AAGGTACCCT 
CACCTGAGTC CCAAGTACAA GGAGTCTTTT GATGTGGGCT GTAACCTCTT TGCCAAGTTT 
TCTGCATACA TTAAGAATAC ACAAAAGGAG GCAAATAAGA ATTTTGAAAA ATCTCTGCTC 
AAAGAATTCA AGCGTCTGGA TGACTACTTA AACACCCCAC TTCTGGATGA AATTGATCCA 
GACAGTGCTG AGGAACCCCC AGTTTCCAGA AGACTATTCT TGGATGGGGA CCAGCTAACA 
CTGGCTGATT GTAGCTTGTT ACCCAAGCTG AACATTATTA AAGTTGCTGC CAAGAAATAT 
CGTGACTTTG ACATTCCAGC AGAATTCTCA GGAGTCTGGC GTTATCTCCA CAATGCCTAT 
GCCCGTGAAG AATTTACCCA CACGTGTCCT GAAGACAAAG AAATTGAAAA TACTTACGCA 
AATGTGGCTA AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280.3
CDS	255..998
Translation	MSGLRPGTQVDPEIELFVKAGSDGESIGNCPFCQRLFMILWLKGVKFNVTTVDMTRKPEELKDLAPGTNPPFLVYNKELKTDFIKIEEFLEQTLAPPRYPHLSPKYKESFDVGCNLFAKFSAYIKNTQKEANKNFEKSLLKEFKRLDDYLNTPLLDEIDPDSAEEPPVSRRLFLDGDQLTLADCSLLPKLNIIKVAAKKYRDFDIPAEFSGVWRYLHNAYAREEFTHTCPEDKEIENTYANVAKQKS

Target ORF information:

RefSeq Version	NM_001289.5
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride intracellular channel 2 (CLIC2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289.5

ORF Insert Sequence:

ATGTCAGGCC TGCGGCCCGG CACTCAAGTG GACCCTGAGA TTGAGCTTTT TGTAAAGGCT 
GGAAGTGATG GAGAGAGTAT TGGAAACTGT CCCTTTTGCC AACGCCTTTT CATGATCCTC 
TGGCTTAAAG GAGTTAAATT TAATGTGACA ACTGTTGACA TGACCAGAAA GCCTGAAGAA 
CTAAAGGACT TAGCCCCAGG TACCAATCCT CCGTTCCTGG TGTATAACAA GGAGTTGAAA 
ACAGACTTCA TTAAAATTGA GGAGTTTTTA GAACAAACCC TGGCTCCTCC AAGGTACCCT 
CACCTGAGTC CCAAGTACAA GGAGTCTTTT GATGTGGGCT GTAACCTCTT TGCCAAGTTT 
TCTGCATACA TTAAGAATAC ACAAAAGGAG GCAAATAAGA ATTTTGAAAA ATCTCTGCTC 
AAAGAATTCA AGCGTCTGGA TGACTACTTA AACACCCCAC TTCTGGATGA AATTGATCCA 
GACAGTGCTG AGGAACCCCC AGTTTCCAGA AGACTATTCT TGGATGGGGA CCAGCTAACA 
CTGGCTGATT GTAGCTTGTT ACCCAAGCTG AACATTATTA AAGTTGCTGC CAAGAAATAT 
CGTGACTTTG ACATTCCAGC AGAATTCTCA GGAGTCTGGC GTTATCTCCA CAATGCCTAT 
GCCCGTGAAG AATTTACCCA CACGTGTCCT GAAGACAAAG AAATTGAAAA TACTTACGCA 
AATGTGGCTA AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tilapia and human CLIC2 structures are highly conserved.
Biochemical and biophysical research communications495(2)1752-1757(2018 Jan)
Zeng J,Li Z,Lui EY,Lam SH,Swaminathan K

XLID-causing mutations and associated genes challenged in light of data from large-scale human exome sequencing.
American journal of human genetics93(2)368-83(2013 Aug)
Piton A,Redin C,Mandel JL

An X-linked channelopathy with cardiomegaly due to a CLIC2 mutation enhancing ryanodine receptor channel activity.
Human molecular genetics21(20)4497-507(2012 Oct)
Takano K,Liu D,Tarpey P,Gallant E,Lam A,Witham S,Alexov E,Chaubey A,Stevenson RE,Schwartz CE,Board PG,Dulhunty AF

A missense mutation in CLIC2 associated with intellectual disability is predicted by in silico modeling to affect protein stability and dynamics.
Proteins79(8)2444-54(2011 Aug)
Witham S,Takano K,Schwartz C,Alexov E

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CLIC2 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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