CLCNKB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLCNKB
Entrez Gene ID	1188
Full Name	chloride voltage-gated channel Kb
Synonyms	CLCKB,ClC-K2,ClC-Kb
General protein information	Preferred Names chloride voltage-gated channel Kb Names chloride channel protein ClC-Kb chloride channel, kidney, B chloride channel, voltage-sensitive Kb
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.13
Summary	The protein encoded by this gene is a member of the family of voltage-gated chloride channels. Chloride channels have several functions, including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. This gene is expressed predominantly in the kidney and may be important for renal salt reabsorption. Mutations in this gene are associated with autosomal recessive Bartter syndrome type 3 (BS3). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009].
Disorder MIM:	602023
Disorder Html:	Bartter syndrome, type 3, 607364 (3); Bartter syndrome, type 4b, digenic, 613090 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000085.5	NP_000076.2	chloride channel protein ClC-Kb isoform 1
NM_000085.4	NP_000076.2	chloride channel protein ClC-Kb isoform 1
NM_001165945.2	NP_001159417.2	chloride channel protein ClC-Kb isoform 2

Pathways from BioSystems

Homologies

	CLCNKB	XP_001091784.2
Canis lupus familiaris (dog)	CLCNKA	XP_544547.2
Bos taurus (cattle)	CLCNKA	XP_005203433.1
Rattus norvegicus (Norway rat)	Clcnka	NP_445779.1
Homo sapiens (human)	CLCNKB	NP_000076.2
Mus musculus (house mouse)	Clcnka	NP_001139779.1
Pan troglodytes (chimpanzee)	CLCNKB	XP_003949353.1
Gallus gallus (chicken)	CLCNKB	XP_004947529.1
Danio rerio (zebrafish)	clcnk	NP_956676.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional severity of CLCNKB mutations correlates with phenotypes in patients with classic Bartter's syndrome.
Cheng CJ, Lo YF, Chen JC, Huang CL, Lin SH
The Journal of physiology595(16)5573-5586(2017 Aug)

Mutations in SLC12A3 and CLCNKB and Their Correlation with Clinical Phenotype in Patients with Gitelman and Gitelman-like Syndrome.
Lee JW, Lee J, Heo NJ, Cheong HI, Han JS
Journal of Korean medical science31(1)47-54(2016 Jan)

Carboxyl-terminal Truncations of ClC-Kb Abolish Channel Activation by Barttin Via Modified Common Gating and Trafficking.
St?lting G, Bungert-Pl?mke S, Franzen A, Fahlke C
The Journal of biological chemistry290(51)30406-16(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Five patients had 1 or more mutations in CLCNKB, of whom 3 had homozygous mutations and 2 had single heterozygous mutations and only in CLCNKB had hypocalciuria.
Title: Mutations in SLC12A3 and CLCNKB and Their Correlation with Clinical Phenotype in Patients with Gitelman and Gitelman-like Syndrome.

results demonstrate that the carboxyl terminus of hClC-Kb is not part of the binding site for barttin, but functionally modifies the interplay with barttin.
Title: Carboxyl-terminal Truncations of ClC-Kb Abolish Channel Activation by Barttin Via Modified Common Gating and Trafficking.

These results demonstrate that mutations in a cluster of hydrophobic residues within transmembrane domain 1 affect barttin-CLC-K interaction and impair gating modification by the accessory subunit
Title: Tryptophan Scanning Mutagenesis Identifies the Molecular Determinants of Distinct Barttin Functions.

R8W and G47R, two naturally occurring barttin mutations identified in patients with Bartter syndrome type IV, reduce barttin palmitoylation and CLC-K/barttin channel activity.
Title: Human CLC-K Channels Require Palmitoylation of Their Accessory Subunit Barttin to Be Functional.

Case Report: 2 mutations in the CLCNKB gene, leading to a molecular diagnosis of Bartter syndrome type III in case of sudden infant death.
Title: Whole-Exome Sequencing Reveals CLCNKB Mutations in a Case of Sudden Unexpected Infant Death.

The following CLCNKB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLCNKB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10515	NM_001165945.2 Latest version!	Homo sapiens chloride voltage-gated channel Kb (CLCNKB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00
OHu20894	NM_000085.5 Latest version!	Homo sapiens chloride voltage-gated channel Kb (CLCNKB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu20894	NM_000085.4	Homo sapiens chloride voltage-gated channel Kb (CLCNKB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$482.30 $689.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLCNKB ( NM_001165945.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159417.2 Homo sapiens chloride voltage-gated channel Kb (CLCNKB), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10515
Clone ID Related Accession (Same CDS sequence)	NM_001165945.2
Accession Version	NM_001165945.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	chloride channel protein ClC-Kb isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA424588.1, DA369686.1, AK098217.1 and AL355994.11. On or before Feb 19, 2014 this sequence version replaced XM_006710070.1, XM_003960900.1, NM_001165945.1. Transcript Variant: This variant (2) has a different 5' UTR, uses an alternate splice site in the 3' coding region, and initiates translation from a different start codon compared to variant 1. This results in a shorter isoform (2) with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK098217.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTGCC CTCCCCTCCT GTCTGTCCCT GTCCGGGCTG CAGGAGAGCA GGACAGATGG 
GTCAGGGAGG AGGTGACATG GGGAGGGGGT CCTACAGTCA CAGGTGGGTG GGGGTGGAGG 
GCCCACCTGA GATCAGTGTC GCCCCCAGGC GTCCTGTTCA GCATCGAGGT CATGTCTTCC 
CACTTCTCTG TCTGGGATTA CTGGAGGGGC TTCTTTGCGG CCACCTGCGG GGCCTTCATG 
TTCCGGCTCC TGGCGGTCTT CAACAGCGAG CAGGAGACCA TCACCTCCCT CTACAAGACC 
AGTTTCCGGG TGGACGTTCC CTTCGACCTG CCTGAGATCT TCTTTTTTGT GGCGCTGGGG 
GGTCTCTGTG GCATCCTGGG CAGCGCTTAC CTCTTCTGTC AGCGAATCTT CTTTGGCTTC 
ATCAGGAACA ATAGGTTCAG CTCCAAACTG CTGGCCACCA GCAAGCCTGT GTACTCCGCT 
CTGGCCACCT TGGTTCTCGC CTCCATCACC TACCCACCCA GCGCCGGCCG CTTCCTAGCT 
TCTCGGCTGT CCATGAAGCA GCATCTGGAC TCGCTGTTCG ACAACCACTC CTGGGCGCTG 
ATGACCCAGA ACTCCAGCCC ACCCTGGCCC GAGGAGCTCG ACCCCCAGCA CCTGTGGTGG 
GAATGGTACC ACCCGCGGTT CACCATCTTT GGGACCCTTG CCTTCTTCCT GGTTATGAAG 
TTCTGGATGC TGATTCTGGC CACCACCATC CCCATGCCTG CCGGGTACTT CATGCCCATC 
TTTGTCTATG GAGCTGCTAT CGGGCGCCTC TTTGGGGAGA CTCTCTCTTT TATCTTCCCT 
GAGGGCATCG TGGCTGGAGG GATCACCAAT CCCATCATGC CAGGGGGGTA TGCTCTGGCA 
GGGGCTGCAG CCTTCTCAGG GGCTGTGACC CACACCATCT CCACGGCGCT GCTGGCCTTC 
GAGGTGACCG GCCAGATAGT GCATGCACTG CCCGTGCTGA TGGCGGTGCT GGCAGCCAAC 
GCCATTGCAC AGAGCTGCCA GCCCTCCTTC TATGATGGCA CCGTCATTGT CAAGAAGCTG 
CCATACCTGC CACGGATTCT GGGCCGCAAC ATCGGTTCCC ACCGCGTGAG GGTGGAGCAC 
TTCATGAACC ACAGCATCAC CACACTGGCC AAGGACATGC CACTGGAGGA GGTGGTCAAG 
GTTGTGACCT CCACAGACGT GGCCAAGTAT CCCCTGGTGG AGAGCACAGA GTCCCAGATC 
CTGGTGGGCA TAGTGCGAAG GGCCCAGCTG GTGCAGGCCC TGAAGGCTGA GCCTCCTTCC 
TGGGCTCCTG GACACCAGTG TCTCCAGGAC ATCTTGGCTG CAGGCTGCCC CACAGAACCA 
GTGACCCTGA AGCTGTCCCC AGAGACTTCC CTGCATGAGG CACACAACCT CTTTGAGCTG 
TTGAACCTTC ATTCCCTCTT TGTGACGTCG CGGGGCAGAG CTGTGGGCTG CGTGTCCTGG 
GTGGAGATGA AGAAAGCAAT TTCCAACCTG ACAAATCCGC CAGCCCCAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159417.2
CDS	211..1764
Translation	MPCPPLLSVPVRAAGEQDRWVREEVTWGGGPTVTGGWGWRAHLRSVSPPGVLFSIEVMSSHFSVWDYWRGFFAATCGAFMFRLLAVFNSEQETITSLYKTSFRVDVPFDLPEIFFFVALGGLCGILGSAYLFCQRIFFGFIRNNRFSSKLLATSKPVYSALATLVLASITYPPSAGRFLASRLSMKQHLDSLFDNHSWALMTQNSSPPWPEELDPQHLWWEWYHPRFTIFGTLAFFLVMKFWMLILATTIPMPAGYFMPIFVYGAAIGRLFGETLSFIFPEGIVAGGITNPIMPGGYALAGAAAFSGAVTHTISTALLAFEVTGQIVHALPVLMAVLAANAIAQSCQPSFYDGTVIVKKLPYLPRILGRNIGSHRVRVEHFMNHSITTLAKDMPLEEVVKVVTSTDVAKYPLVESTESQILVGIVRRAQLVQALKAEPPSWAPGHQCLQDILAAGCPTEPVTLKLSPETSLHEAHNLFELLNLHSLFVTSRGRAVGCVSWVEMKKAISNLTNPPAPK

Target ORF information:

RefSeq Version	NM_001165945.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel Kb (CLCNKB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165945.2

ORF Insert Sequence:

ATGCCCTGCC CTCCCCTCCT GTCTGTCCCT GTCCGGGCTG CAGGAGAGCA GGACAGATGG 
GTCAGGGAGG AGGTGACATG GGGAGGGGGT CCTACAGTCA CAGGTGGGTG GGGGTGGAGG 
GCCCACCTGA GATCAGTGTC GCCCCCAGGC GTCCTGTTCA GCATCGAGGT CATGTCTTCC 
CACTTCTCTG TCTGGGATTA CTGGAGGGGC TTCTTTGCGG CCACCTGCGG GGCCTTCATG 
TTCCGGCTCC TGGCGGTCTT CAACAGCGAG CAGGAGACCA TCACCTCCCT CTACAAGACC 
AGTTTCCGGG TGGACGTTCC CTTCGACCTG CCTGAGATCT TCTTTTTTGT GGCGCTGGGG 
GGTCTCTGTG GCATCCTGGG CAGCGCTTAC CTCTTCTGTC AGCGAATCTT CTTTGGCTTC 
ATCAGGAACA ATAGGTTCAG CTCCAAACTG CTGGCCACCA GCAAGCCTGT GTACTCCGCT 
CTGGCCACCT TGGTTCTCGC CTCCATCACC TACCCACCCA GCGCCGGCCG CTTCCTAGCT 
TCTCGGCTGT CCATGAAGCA GCATCTGGAC TCGCTGTTCG ACAACCACTC CTGGGCGCTG 
ATGACCCAGA ACTCCAGCCC ACCCTGGCCC GAGGAGCTCG ACCCCCAGCA CCTGTGGTGG 
GAATGGTACC ACCCGCGGTT CACCATCTTT GGGACCCTTG CCTTCTTCCT GGTTATGAAG 
TTCTGGATGC TGATTCTGGC CACCACCATC CCCATGCCTG CCGGGTACTT CATGCCCATC 
TTTGTCTATG GAGCTGCTAT CGGGCGCCTC TTTGGGGAGA CTCTCTCTTT TATCTTCCCT 
GAGGGCATCG TGGCTGGAGG GATCACCAAT CCCATCATGC CAGGGGGGTA TGCTCTGGCA 
GGGGCTGCAG CCTTCTCAGG GGCTGTGACC CACACCATCT CCACGGCGCT GCTGGCCTTC 
GAGGTGACCG GCCAGATAGT GCATGCACTG CCCGTGCTGA TGGCGGTGCT GGCAGCCAAC 
GCCATTGCAC AGAGCTGCCA GCCCTCCTTC TATGATGGCA CCGTCATTGT CAAGAAGCTG 
CCATACCTGC CACGGATTCT GGGCCGCAAC ATCGGTTCCC ACCGCGTGAG GGTGGAGCAC 
TTCATGAACC ACAGCATCAC CACACTGGCC AAGGACATGC CACTGGAGGA GGTGGTCAAG 
GTTGTGACCT CCACAGACGT GGCCAAGTAT CCCCTGGTGG AGAGCACAGA GTCCCAGATC 
CTGGTGGGCA TAGTGCGAAG GGCCCAGCTG GTGCAGGCCC TGAAGGCTGA GCCTCCTTCC 
TGGGCTCCTG GACACCAGTG TCTCCAGGAC ATCTTGGCTG CAGGCTGCCC CACAGAACCA 
GTGACCCTGA AGCTGTCCCC AGAGACTTCC CTGCATGAGG CACACAACCT CTTTGAGCTG 
TTGAACCTTC ATTCCCTCTT TGTGACGTCG CGGGGCAGAG CTGTGGGCTG CGTGTCCTGG 
GTGGAGATGA AGAAAGCAAT TTCCAACCTG ACAAATCCGC CAGCCCCAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCNKB ( NM_000085.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000076.2 Homo sapiens chloride voltage-gated channel Kb (CLCNKB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20894
Clone ID Related Accession (Same CDS sequence)	NM_000085.5 , NM_000085.4
Accession Version	NM_000085.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	chloride channel protein ClC-Kb isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA624942.1, DA627682.1, DA633177.1, AK292335.1, AL355994.11 and AK098217.1. This sequence is a reference standard in the RefSeqGene project. On or before Jul 26, 2012 this sequence version replaced XM_003846621.1, NM_000085.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033806.1, AK292335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGT TTGTGGGGCT GCGTGAAGGC TCCTCAGGGA ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCG CATCCGCCGA GGCATCCGAG GTGGCCTGGA GTGGCTGAAG 
CAGAAGCTCT TCCGCCTGGG CGAGGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTGTGCCAT GGACTTGGCT GTTGAGAGTG TGGTCCGAGC GCACCAGTGG 
CTGTACAGGG AGATTGGGGA CAGCCACCTG CTCCGGTATC TCTCCTGGAC TGTGTACCCT 
GTGGCCCTCG TCTCTTTCTC TTCAGGCTTC TCTCAGAGCA TCACACCCTC CTCTGGAGGT 
TCTGGAATCC CGGAGGTGAA GACCATGTTG GCGGGTGTGG TCTTGGAGGA CTACCTGGAT 
ATCAAGAACT TTGGGGCCAA AGTGGTGGGC CTCTCCTGCA CCCTGGCCTG TGGCAGCACC 
CTCTTCCTCG GGAAAGTGGG CCCTTTCGTG CACCTGTCTG TGATGATGGC TGCCTACCTG 
GGCCGTGTGC GCACCACGAC CATCGGGGAG CCTGAGAACA AGAGCAAGCA AAACGAAATG 
CTGGTGGCAG CGGCGGCAGT GGGCGTGGCC ACAGTCTTTG CAGCTCCCTT CAGCGGCGTC 
CTGTTCAGCA TCGAGGTCAT GTCTTCCCAC TTCTCTGTCT GGGATTACTG GAGGGGCTTC 
TTTGCGGCCA CCTGCGGGGC CTTCATGTTC CGGCTCCTGG CGGTCTTCAA CAGCGAGCAG 
GAGACCATCA CCTCCCTCTA CAAGACCAGT TTCCGGGTGG ACGTTCCCTT CGACCTGCCT 
GAGATCTTCT TTTTTGTGGC GCTGGGGGGT CTCTGTGGCA TCCTGGGCAG CGCTTACCTC 
TTCTGTCAGC GAATCTTCTT TGGCTTCATC AGGAACAATA GGTTCAGCTC CAAACTGCTG 
GCCACCAGCA AGCCTGTGTA CTCCGCTCTG GCCACCTTGG TTCTCGCCTC CATCACCTAC 
CCACCCAGCG CCGGCCGCTT CCTAGCTTCT CGGCTGTCCA TGAAGCAGCA TCTGGACTCG 
CTGTTCGACA ACCACTCCTG GGCGCTGATG ACCCAGAACT CCAGCCCACC CTGGCCCGAG 
GAGCTCGACC CCCAGCACCT GTGGTGGGAA TGGTACCACC CGCGGTTCAC CATCTTTGGG 
ACCCTTGCCT TCTTCCTGGT TATGAAGTTC TGGATGCTGA TTCTGGCCAC CACCATCCCC 
ATGCCTGCCG GGTACTTCAT GCCCATCTTT GTCTATGGAG CTGCTATCGG GCGCCTCTTT 
GGGGAGACTC TCTCTTTTAT CTTCCCTGAG GGCATCGTGG CTGGAGGGAT CACCAATCCC 
ATCATGCCAG GGGGGTATGC TCTGGCAGGG GCTGCAGCCT TCTCAGGGGC TGTGACCCAC 
ACCATCTCCA CGGCGCTGCT GGCCTTCGAG GTGACCGGCC AGATAGTGCA TGCACTGCCC 
GTGCTGATGG CGGTGCTGGC AGCCAACGCC ATTGCACAGA GCTGCCAGCC CTCCTTCTAT 
GATGGCACCG TCATTGTCAA GAAGCTGCCA TACCTGCCAC GGATTCTGGG CCGCAACATC 
GGTTCCCACC GCGTGAGGGT GGAGCACTTC ATGAACCACA GCATCACCAC ACTGGCCAAG 
GACATGCCAC TGGAGGAGGT GGTCAAGGTT GTGACCTCCA CAGACGTGGC CAAGTATCCC 
CTGGTGGAGA GCACAGAGTC CCAGATCCTG GTGGGCATAG TGCGAAGGGC CCAGCTGGTG 
CAGGCCCTGA AGGCTGAGCC TCCTTCCTGG GCTCCTGGAC ACCAGCAGTG TCTCCAGGAC 
ATCTTGGCTG CAGGCTGCCC CACAGAACCA GTGACCCTGA AGCTGTCCCC AGAGACTTCC 
CTGCATGAGG CACACAACCT CTTTGAGCTG TTGAACCTTC ATTCCCTCTT TGTGACGTCG 
CGGGGCAGAG CTGTGGGCTG CGTGTCCTGG GTGGAGATGA AGAAAGCAAT TTCCAACCTG 
ACAAATCCGC CAGCCCCAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000076.2
CDS	153..2216
Translation	MEEFVGLREGSSGNPVTLQELWGPCPRIRRGIRGGLEWLKQKLFRLGEDWYFLMTLGVLMALVSCAMDLAVESVVRAHQWLYREIGDSHLLRYLSWTVYPVALVSFSSGFSQSITPSSGGSGIPEVKTMLAGVVLEDYLDIKNFGAKVVGLSCTLACGSTLFLGKVGPFVHLSVMMAAYLGRVRTTTIGEPENKSKQNEMLVAAAAVGVATVFAAPFSGVLFSIEVMSSHFSVWDYWRGFFAATCGAFMFRLLAVFNSEQETITSLYKTSFRVDVPFDLPEIFFFVALGGLCGILGSAYLFCQRIFFGFIRNNRFSSKLLATSKPVYSALATLVLASITYPPSAGRFLASRLSMKQHLDSLFDNHSWALMTQNSSPPWPEELDPQHLWWEWYHPRFTIFGTLAFFLVMKFWMLILATTIPMPAGYFMPIFVYGAAIGRLFGETLSFIFPEGIVAGGITNPIMPGGYALAGAAAFSGAVTHTISTALLAFEVTGQIVHALPVLMAVLAANAIAQSCQPSFYDGTVIVKKLPYLPRILGRNIGSHRVRVEHFMNHSITTLAKDMPLEEVVKVVTSTDVAKYPLVESTESQILVGIVRRAQLVQALKAEPPSWAPGHQQCLQDILAAGCPTEPVTLKLSPETSLHEAHNLFELLNLHSLFVTSRGRAVGCVSWVEMKKAISNLTNPPAPK

Target ORF information:

RefSeq Version	NM_000085.4
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel Kb (CLCNKB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000085.4

ORF Insert Sequence:

ATGGAGGAGT TTGTGGGGCT GCGTGAAGGC TCCTCAGGGA ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCG CATCCGCCGA GGCATCCGAG GTGGCCTGGA GTGGCTGAAG 
CAGAAGCTCT TCCGCCTGGG CGAGGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTGTGCCAT GGACTTGGCT GTTGAGAGTG TGGTCCGAGC GCACCAGTGG 
CTGTACAGGG AGATTGGGGA CAGCCACCTG CTCCGGTATC TCTCCTGGAC TGTGTACCCT 
GTGGCCCTCG TCTCTTTCTC TTCAGGCTTC TCTCAGAGCA TCACACCCTC CTCTGGAGGT 
TCTGGAATCC CGGAGGTGAA GACCATGTTG GCGGGTGTGG TCTTGGAGGA CTACCTGGAT 
ATCAAGAACT TTGGGGCCAA AGTGGTGGGC CTCTCCTGCA CCCTGGCCTG TGGCAGCACC 
CTCTTCCTCG GGAAAGTGGG CCCTTTCGTG CACCTGTCTG TGATGATGGC TGCCTACCTG 
GGCCGTGTGC GCACCACGAC CATCGGGGAG CCTGAGAACA AGAGCAAGCA AAACGAAATG 
CTGGTGGCAG CGGCGGCAGT GGGCGTGGCC ACAGTCTTTG CAGCTCCCTT CAGCGGCGTC 
CTGTTCAGCA TCGAGGTCAT GTCTTCCCAC TTCTCTGTCT GGGATTACTG GAGGGGCTTC 
TTTGCGGCCA CCTGCGGGGC CTTCATGTTC CGGCTCCTGG CGGTCTTCAA CAGCGAGCAG 
GAGACCATCA CCTCCCTCTA CAAGACCAGT TTCCGGGTGG ACGTTCCCTT CGACCTGCCT 
GAGATCTTCT TTTTTGTGGC GCTGGGGGGT CTCTGTGGCA TCCTGGGCAG CGCTTACCTC 
TTCTGTCAGC GAATCTTCTT TGGCTTCATC AGGAACAATA GGTTCAGCTC CAAACTGCTG 
GCCACCAGCA AGCCTGTGTA CTCCGCTCTG GCCACCTTGG TTCTCGCCTC CATCACCTAC 
CCACCCAGCG CCGGCCGCTT CCTAGCTTCT CGGCTGTCCA TGAAGCAGCA TCTGGACTCG 
CTGTTCGACA ACCACTCCTG GGCGCTGATG ACCCAGAACT CCAGCCCACC CTGGCCCGAG 
GAGCTCGACC CCCAGCACCT GTGGTGGGAA TGGTACCACC CGCGGTTCAC CATCTTTGGG 
ACCCTTGCCT TCTTCCTGGT TATGAAGTTC TGGATGCTGA TTCTGGCCAC CACCATCCCC 
ATGCCTGCCG GGTACTTCAT GCCCATCTTT GTCTATGGAG CTGCTATCGG GCGCCTCTTT 
GGGGAGACTC TCTCTTTTAT CTTCCCTGAG GGCATCGTGG CTGGAGGGAT CACCAATCCC 
ATCATGCCAG GGGGGTATGC TCTGGCAGGG GCTGCAGCCT TCTCAGGGGC TGTGACCCAC 
ACCATCTCCA CGGCGCTGCT GGCCTTCGAG GTGACCGGCC AGATAGTGCA TGCACTGCCC 
GTGCTGATGG CGGTGCTGGC AGCCAACGCC ATTGCACAGA GCTGCCAGCC CTCCTTCTAT 
GATGGCACCG TCATTGTCAA GAAGCTGCCA TACCTGCCAC GGATTCTGGG CCGCAACATC 
GGTTCCCACC GCGTGAGGGT GGAGCACTTC ATGAACCACA GCATCACCAC ACTGGCCAAG 
GACATGCCAC TGGAGGAGGT GGTCAAGGTT GTGACCTCCA CAGACGTGGC CAAGTATCCC 
CTGGTGGAGA GCACAGAGTC CCAGATCCTG GTGGGCATAG TGCGAAGGGC CCAGCTGGTG 
CAGGCCCTGA AGGCTGAGCC TCCTTCCTGG GCTCCTGGAC ACCAGCAGTG TCTCCAGGAC 
ATCTTGGCTG CAGGCTGCCC CACAGAACCA GTGACCCTGA AGCTGTCCCC AGAGACTTCC 
CTGCATGAGG CACACAACCT CTTTGAGCTG TTGAACCTTC ATTCCCTCTT TGTGACGTCG 
CGGGGCAGAG CTGTGGGCTG CGTGTCCTGG GTGGAGATGA AGAAAGCAAT TTCCAACCTG 
ACAAATCCGC CAGCCCCAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCNKB ( NM_000085.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000076.2 Homo sapiens chloride voltage-gated channel Kb (CLCNKB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20894
Clone ID Related Accession (Same CDS sequence)	NM_000085.5 , NM_000085.4
Accession Version	NM_000085.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	chloride channel protein ClC-Kb isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355994.11. On Aug 31, 2019 this sequence version replaced NM_000085.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033806.1, AK292335.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGT TTGTGGGGCT GCGTGAAGGC TCCTCAGGGA ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCG CATCCGCCGA GGCATCCGAG GTGGCCTGGA GTGGCTGAAG 
CAGAAGCTCT TCCGCCTGGG CGAGGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTGTGCCAT GGACTTGGCT GTTGAGAGTG TGGTCCGAGC GCACCAGTGG 
CTGTACAGGG AGATTGGGGA CAGCCACCTG CTCCGGTATC TCTCCTGGAC TGTGTACCCT 
GTGGCCCTCG TCTCTTTCTC TTCAGGCTTC TCTCAGAGCA TCACACCCTC CTCTGGAGGT 
TCTGGAATCC CGGAGGTGAA GACCATGTTG GCGGGTGTGG TCTTGGAGGA CTACCTGGAT 
ATCAAGAACT TTGGGGCCAA AGTGGTGGGC CTCTCCTGCA CCCTGGCCTG TGGCAGCACC 
CTCTTCCTCG GGAAAGTGGG CCCTTTCGTG CACCTGTCTG TGATGATGGC TGCCTACCTG 
GGCCGTGTGC GCACCACGAC CATCGGGGAG CCTGAGAACA AGAGCAAGCA AAACGAAATG 
CTGGTGGCAG CGGCGGCAGT GGGCGTGGCC ACAGTCTTTG CAGCTCCCTT CAGCGGCGTC 
CTGTTCAGCA TCGAGGTCAT GTCTTCCCAC TTCTCTGTCT GGGATTACTG GAGGGGCTTC 
TTTGCGGCCA CCTGCGGGGC CTTCATGTTC CGGCTCCTGG CGGTCTTCAA CAGCGAGCAG 
GAGACCATCA CCTCCCTCTA CAAGACCAGT TTCCGGGTGG ACGTTCCCTT CGACCTGCCT 
GAGATCTTCT TTTTTGTGGC GCTGGGGGGT CTCTGTGGCA TCCTGGGCAG CGCTTACCTC 
TTCTGTCAGC GAATCTTCTT TGGCTTCATC AGGAACAATA GGTTCAGCTC CAAACTGCTG 
GCCACCAGCA AGCCTGTGTA CTCCGCTCTG GCCACCTTGG TTCTCGCCTC CATCACCTAC 
CCACCCAGCG CCGGCCGCTT CCTAGCTTCT CGGCTGTCCA TGAAGCAGCA TCTGGACTCG 
CTGTTCGACA ACCACTCCTG GGCGCTGATG ACCCAGAACT CCAGCCCACC CTGGCCCGAG 
GAGCTCGACC CCCAGCACCT GTGGTGGGAA TGGTACCACC CGCGGTTCAC CATCTTTGGG 
ACCCTTGCCT TCTTCCTGGT TATGAAGTTC TGGATGCTGA TTCTGGCCAC CACCATCCCC 
ATGCCTGCCG GGTACTTCAT GCCCATCTTT GTCTATGGAG CTGCTATCGG GCGCCTCTTT 
GGGGAGACTC TCTCTTTTAT CTTCCCTGAG GGCATCGTGG CTGGAGGGAT CACCAATCCC 
ATCATGCCAG GGGGGTATGC TCTGGCAGGG GCTGCAGCCT TCTCAGGGGC TGTGACCCAC 
ACCATCTCCA CGGCGCTGCT GGCCTTCGAG GTGACCGGCC AGATAGTGCA TGCACTGCCC 
GTGCTGATGG CGGTGCTGGC AGCCAACGCC ATTGCACAGA GCTGCCAGCC CTCCTTCTAT 
GATGGCACCG TCATTGTCAA GAAGCTGCCA TACCTGCCAC GGATTCTGGG CCGCAACATC 
GGTTCCCACC GCGTGAGGGT GGAGCACTTC ATGAACCACA GCATCACCAC ACTGGCCAAG 
GACATGCCAC TGGAGGAGGT GGTCAAGGTT GTGACCTCCA CAGACGTGGC CAAGTATCCC 
CTGGTGGAGA GCACAGAGTC CCAGATCCTG GTGGGCATAG TGCGAAGGGC CCAGCTGGTG 
CAGGCCCTGA AGGCTGAGCC TCCTTCCTGG GCTCCTGGAC ACCAGCAGTG TCTCCAGGAC 
ATCTTGGCTG CAGGCTGCCC CACAGAACCA GTGACCCTGA AGCTGTCCCC AGAGACTTCC 
CTGCATGAGG CACACAACCT CTTTGAGCTG TTGAACCTTC ATTCCCTCTT TGTGACGTCG 
CGGGGCAGAG CTGTGGGCTG CGTGTCCTGG GTGGAGATGA AGAAAGCAAT TTCCAACCTG 
ACAAATCCGC CAGCCCCAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000076.2
CDS	107..2170
Translation	MEEFVGLREGSSGNPVTLQELWGPCPRIRRGIRGGLEWLKQKLFRLGEDWYFLMTLGVLMALVSCAMDLAVESVVRAHQWLYREIGDSHLLRYLSWTVYPVALVSFSSGFSQSITPSSGGSGIPEVKTMLAGVVLEDYLDIKNFGAKVVGLSCTLACGSTLFLGKVGPFVHLSVMMAAYLGRVRTTTIGEPENKSKQNEMLVAAAAVGVATVFAAPFSGVLFSIEVMSSHFSVWDYWRGFFAATCGAFMFRLLAVFNSEQETITSLYKTSFRVDVPFDLPEIFFFVALGGLCGILGSAYLFCQRIFFGFIRNNRFSSKLLATSKPVYSALATLVLASITYPPSAGRFLASRLSMKQHLDSLFDNHSWALMTQNSSPPWPEELDPQHLWWEWYHPRFTIFGTLAFFLVMKFWMLILATTIPMPAGYFMPIFVYGAAIGRLFGETLSFIFPEGIVAGGITNPIMPGGYALAGAAAFSGAVTHTISTALLAFEVTGQIVHALPVLMAVLAANAIAQSCQPSFYDGTVIVKKLPYLPRILGRNIGSHRVRVEHFMNHSITTLAKDMPLEEVVKVVTSTDVAKYPLVESTESQILVGIVRRAQLVQALKAEPPSWAPGHQQCLQDILAAGCPTEPVTLKLSPETSLHEAHNLFELLNLHSLFVTSRGRAVGCVSWVEMKKAISNLTNPPAPK

Target ORF information:

RefSeq Version	NM_000085.5
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel Kb (CLCNKB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000085.5

ORF Insert Sequence:

ATGGAGGAGT TTGTGGGGCT GCGTGAAGGC TCCTCAGGGA ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCG CATCCGCCGA GGCATCCGAG GTGGCCTGGA GTGGCTGAAG 
CAGAAGCTCT TCCGCCTGGG CGAGGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTGTGCCAT GGACTTGGCT GTTGAGAGTG TGGTCCGAGC GCACCAGTGG 
CTGTACAGGG AGATTGGGGA CAGCCACCTG CTCCGGTATC TCTCCTGGAC TGTGTACCCT 
GTGGCCCTCG TCTCTTTCTC TTCAGGCTTC TCTCAGAGCA TCACACCCTC CTCTGGAGGT 
TCTGGAATCC CGGAGGTGAA GACCATGTTG GCGGGTGTGG TCTTGGAGGA CTACCTGGAT 
ATCAAGAACT TTGGGGCCAA AGTGGTGGGC CTCTCCTGCA CCCTGGCCTG TGGCAGCACC 
CTCTTCCTCG GGAAAGTGGG CCCTTTCGTG CACCTGTCTG TGATGATGGC TGCCTACCTG 
GGCCGTGTGC GCACCACGAC CATCGGGGAG CCTGAGAACA AGAGCAAGCA AAACGAAATG 
CTGGTGGCAG CGGCGGCAGT GGGCGTGGCC ACAGTCTTTG CAGCTCCCTT CAGCGGCGTC 
CTGTTCAGCA TCGAGGTCAT GTCTTCCCAC TTCTCTGTCT GGGATTACTG GAGGGGCTTC 
TTTGCGGCCA CCTGCGGGGC CTTCATGTTC CGGCTCCTGG CGGTCTTCAA CAGCGAGCAG 
GAGACCATCA CCTCCCTCTA CAAGACCAGT TTCCGGGTGG ACGTTCCCTT CGACCTGCCT 
GAGATCTTCT TTTTTGTGGC GCTGGGGGGT CTCTGTGGCA TCCTGGGCAG CGCTTACCTC 
TTCTGTCAGC GAATCTTCTT TGGCTTCATC AGGAACAATA GGTTCAGCTC CAAACTGCTG 
GCCACCAGCA AGCCTGTGTA CTCCGCTCTG GCCACCTTGG TTCTCGCCTC CATCACCTAC 
CCACCCAGCG CCGGCCGCTT CCTAGCTTCT CGGCTGTCCA TGAAGCAGCA TCTGGACTCG 
CTGTTCGACA ACCACTCCTG GGCGCTGATG ACCCAGAACT CCAGCCCACC CTGGCCCGAG 
GAGCTCGACC CCCAGCACCT GTGGTGGGAA TGGTACCACC CGCGGTTCAC CATCTTTGGG 
ACCCTTGCCT TCTTCCTGGT TATGAAGTTC TGGATGCTGA TTCTGGCCAC CACCATCCCC 
ATGCCTGCCG GGTACTTCAT GCCCATCTTT GTCTATGGAG CTGCTATCGG GCGCCTCTTT 
GGGGAGACTC TCTCTTTTAT CTTCCCTGAG GGCATCGTGG CTGGAGGGAT CACCAATCCC 
ATCATGCCAG GGGGGTATGC TCTGGCAGGG GCTGCAGCCT TCTCAGGGGC TGTGACCCAC 
ACCATCTCCA CGGCGCTGCT GGCCTTCGAG GTGACCGGCC AGATAGTGCA TGCACTGCCC 
GTGCTGATGG CGGTGCTGGC AGCCAACGCC ATTGCACAGA GCTGCCAGCC CTCCTTCTAT 
GATGGCACCG TCATTGTCAA GAAGCTGCCA TACCTGCCAC GGATTCTGGG CCGCAACATC 
GGTTCCCACC GCGTGAGGGT GGAGCACTTC ATGAACCACA GCATCACCAC ACTGGCCAAG 
GACATGCCAC TGGAGGAGGT GGTCAAGGTT GTGACCTCCA CAGACGTGGC CAAGTATCCC 
CTGGTGGAGA GCACAGAGTC CCAGATCCTG GTGGGCATAG TGCGAAGGGC CCAGCTGGTG 
CAGGCCCTGA AGGCTGAGCC TCCTTCCTGG GCTCCTGGAC ACCAGCAGTG TCTCCAGGAC 
ATCTTGGCTG CAGGCTGCCC CACAGAACCA GTGACCCTGA AGCTGTCCCC AGAGACTTCC 
CTGCATGAGG CACACAACCT CTTTGAGCTG TTGAACCTTC ATTCCCTCTT TGTGACGTCG 
CGGGGCAGAG CTGTGGGCTG CGTGTCCTGG GTGGAGATGA AGAAAGCAAT TTCCAACCTG 
ACAAATCCGC CAGCCCCAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional severity of CLCNKB mutations correlates with phenotypes in patients with classic Bartter's syndrome.
The Journal of physiology595(16)5573-5586(2017 Aug)
Cheng CJ,Lo YF,Chen JC,Huang CL,Lin SH

Mutations in SLC12A3 and CLCNKB and Their Correlation with Clinical Phenotype in Patients with Gitelman and Gitelman-like Syndrome.
Journal of Korean medical science31(1)47-54(2016 Jan)
Lee JW,Lee J,Heo NJ,Cheong HI,Han JS

Carboxyl-terminal Truncations of ClC-Kb Abolish Channel Activation by Barttin Via Modified Common Gating and Trafficking.
The Journal of biological chemistry290(51)30406-16(2015 Dec)
St?lting G,Bungert-Pl?mke S,Franzen A,Fahlke C

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CLCNKB cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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