CLCNKA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLCNKA
Entrez Gene ID	1187
Full Name	chloride voltage-gated channel Ka
Synonyms	CLCK1,ClC-K1,hClC-Ka
General protein information	Preferred Names chloride voltage-gated channel Ka Names chloride channel protein ClC-Ka chloride channel Ka chloride channel, kidney, A chloride channel, voltage-sensitive Ka
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.13
Summary	This gene is a member of the CLC family of voltage-gated chloride channels. The encoded protein is predicted to have 12 transmembrane domains, and requires a beta subunit called barttin to form a functional channel. It is thought to function in salt reabsorption in the kidney and potassium recycling in the inner ear. The gene is highly similar to CLCNKB, which is located 10 kb downstream from this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	602024
Disorder Html:	Bartter syndrome, type 4b, digenic, 613090 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004070.3	NP_004061.3	chloride channel protein ClC-Ka isoform 1
NM_001257139.1	NP_001244068.1	chloride channel protein ClC-Ka isoform 3
NM_004070.4	NP_004061.3	chloride channel protein ClC-Ka isoform 1
NM_001042704.1	NP_001036169.1	chloride channel protein ClC-Ka isoform 2
NM_001257139.2	NP_001244068.1	chloride channel protein ClC-Ka isoform 3
NM_001042704.2	NP_001036169.1	chloride channel protein ClC-Ka isoform 2

Pathways from BioSystems

Homologies

	CLCNKA	XP_001091558.2
Homo sapiens (human)	CLCNKA	NP_001036169.1
Rattus norvegicus (Norway rat)	Clcnkb	NP_775126.1
Pan troglodytes (chimpanzee)	CLCNKA	XP_525229.3
Mus musculus (house mouse)	Clcnkb	NP_062675.2

Gene Ontology
Bibliography

Related articles in PubMed

Human CLC-K Channels Require Palmitoylation of Their Accessory Subunit Barttin to Be Functional.
Steinke KV, Gorinski N, Wojciechowski D, Todorov V, Guseva D, Ponimaskin E, Fahlke C, Fischer M
The Journal of biological chemistry290(28)17390-400(2015 Jul)

Associations between CLCNKA_B tag SNPs with essential hypertension and interactions between genetic and environmental factors in an island population in China.
Chen X, Zhou B, Hou X, Xing J, Zou S, Wu X, Ma B, Shi J
Clinical and experimental hypertension (New York, N.Y. : 1993)37(7)519-25(2015)

Targeting kidney CLC-K channels: pharmacological profile in a human cell line versus Xenopus oocytes.
Imbrici P, Liantonio A, Gradogna A, Pusch M, Camerino DC
Biochimica et biophysica acta1838(10)2484-91(2014 Oct)

A CLCNKA polymorphism (rs10927887; p.Arg83Gly) previously linked to heart failure is associated with the estimated glomerular filtration rate in the RENASTUR cohort.
Tavira B, G?mez J, Ortega F, Tranche S, D?az-Corte C, Alvarez F, Ortiz A, Santos F, S?nchez-Ni?o MD, Coto E
Gene527(2)670-2(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

R8W and G47R, two naturally occurring barttin mutations identified in patients with Bartter syndrome type IV, reduce barttin palmitoylation and CLC-K/barttin channel activity.
Title: Human CLC-K Channels Require Palmitoylation of Their Accessory Subunit Barttin to Be Functional.

Single loci of tag Single Nucleotide Polymorphisms of CLCNKA_B are not enough to increase the Essential Hypertension susceptibility, the combination of CLCNKA SNP, salt, marine products, meat, edible oil consumption is associated with elevated risk.
Title: Associations between CLCNKA_B tag SNPs with essential hypertension and interactions between genetic and environmental factors in an island population in China.

HEK293 cells the potentiating effect of niflumic acid (NFA) on CLC-Ka/barttin and CLC-Kb/barttin channels seems to be absent while the blocking efficacy of niflumic acid and benzofuran derivatives observed in oocytes is preserved
Title: Targeting kidney CLC-K channels: pharmacological profile in a human cell line versus Xenopus oocytes.

following variables were significantly associated with an estimated glomerular filtration rate: age, type 2 diabetes, total cholesterol, LDL-cholesterol, and the CLCNKA GG genotype
Title: A CLCNKA polymorphism (rs10927887; p.Arg83Gly) previously linked to heart failure is associated with the estimated glomerular filtration rate in the RENASTUR cohort.

The variant CLCNKA risk allele, telegraphed by linked variants in the adjacent HSPB7 gene, uncovers a previously overlooked genetic mechanism affecting the cardio-renal axis.
Title: Loss-of-function DNA sequence variant in the CLCNKA chloride channel implicates the cardio-renal axis in interindividual heart failure risk variation.

The following CLCNKA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLCNKA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19650	NM_004070.4 Latest version!	Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu12112	NM_001257139.2 Latest version!	Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu30105	NM_001042704.2 Latest version!	Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
View Old Accession Versions >>
OHu19650	NM_004070.3	Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu12112	NM_001257139.1	Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu30105	NM_001042704.1	Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLCNKA ( NM_004070.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004061.3 Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19650
Clone ID Related Accession (Same CDS sequence)	NM_004070.3 , NM_004070.4
Accession Version	NM_004070.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	chloride channel protein ClC-Ka isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC053869.1 and Z30643.1. This sequence is a reference standard in the RefSeqGene project. On Aug 16, 2006 this sequence version replaced NM_004070.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290814.1, Z30643.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGT TGGTGGGGCT GCGTGAGGGC TTCTCAGGGG ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCA CATCCGCCGA GCCATCCAAG GTGGCCTGGA GTGGCTAAAG 
CAGAAGGTGT TCCGCCTGGG AGAAGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTATGCCAT GAACTTTGCC ATCGGGTGTG TGGTCCGAGC ACACCAGTGG 
CTGTACAGGG AGATTGGGGA CAGCCACCTG CTCCGGTATC TTTCCTGGAC TGTGTACCCT 
GTGGCCCTCG TCTCTTTCTC CTCAGGCTTC TCCCAGAGCA TCACGCCCTC CTCTGGAGGT 
TCTGGAATCC CGGAGCTGAA GACCATGTTG GCGGGTGTGA TCTTGGAGGA CTACCTGGAT 
ATCAAGAACT TTGGGGCCAA GGTGGTGGGC CTCTCCTGCA CCCTGGCCAC CGGCAGCACC 
CTGTTCCTGG GCAAAGTGGG CCCTTTCGTG CACCTGTCTG TAATGATCGC TGCCTACCTG 
GGCCGTGTGC GCACCACGAC CATCGGGGAG CCTGAGAACA AGAGCAAGCA AAACGAAATG 
CTGGTGGCAG CGGCGGCAGT GGGCGTGGCC ACAGTCTTTG CAGCTCCCTT CAGCGGCGTC 
CTGTTCAGCA TCGAGGTCAT GTCTTCCCAC TTCTCTGTCC GGGATTACTG GAGGGGCTTC 
TTTGCGGCCA CCTGCGGGGC CTTCATATTC CGGCTCCTGG CAGTCTTCAA CAGCGAGCAG 
GAGACCATCA CCTCCCTCTA CAAGACCAGT TTCCGGGTGG ACGTTCCCTT CGACCTGCCT 
GAGATCTTCT TTTTTGTGGC GCTGGGTGGC ATCTGCGGCG TCCTGAGCTG TGCTTACCTC 
TTCTGTCAGC GAACCTTCCT CAGCTTCATC AAGACCAATC GGTACAGCTC CAAACTGCTG 
GCTACTAGCA AGCCTGTGTA CTCCGCTCTG GCCACCTTGC TTCTCGCCTC CATCACCTAC 
CCGCCTGGTG TGGGCCACTT CCTAGCTTCT CGGCTGTCCA TGAAGCAGCA TCTGGACTCG 
CTGTTCGACA ACCACTCCTG GGCGCTGATG ACCCAGAACT CCAGCCCACC CTGGCCCGAG 
GAGCTCGACC CCCAGCACCT TTGGTGGGAA TGGTACCACC CGCGGTTCAC CATCTTTGGG 
ACCCTTGCCT TCTTCCTGGT TATGAAGTTC TGGATGCTGA TTCTGGCCAC CACCATCCCC 
ATGCCTGCCG GGTACTTCAT GCCCATCTTT ATCCTTGGAG CTGCCATCGG GCGCCTCTTG 
GGAGAGGCTC TTGCCGTCGC CTTCCCTGAG GGCATTGTGA CTGGAGGGGT TACCAATCCC 
ATCATGCCCG GGGGGTATGC TCTGGCAGGG GCTGCAGCCT TCTCAGGGGC TGTGACCCAC 
ACCATCTCCA CGGCGCTGCT GGCCTTTGAG CTGACCGGCC AGATAGTGCA TGCACTGCCC 
GTGCTGATGG CGGTGCTGGC AGCCAACGCC ATTGCACAGA GCTGCCAGCC CTCCTTCTAT 
GATGGCACCA TCATTGTCAA GAAGCTGCCA TACCTGCCAC GGATTCTGGG CCGCAACATC 
GGCTCCCACC ATGTGAGGGT GGAGCACTTC ATGAACCACA GCATCACCAC ACTGGCCAAG 
GACACGCCGC TGGAGGAGGT GGTCAAGGTT GTGACCTCCA CAGACGTGAC CGAGTATCCC 
CTGGTGGAGA GCACAGAGTC CCAGATCCTG GTAGGCATCG TGCAGAGGGC CCAGCTGGTG 
CAGGCCCTCC AGGCTGAGCC TCCTTCCAGG GCTCCAGGAC ACCAGCAGTG TCTCCAGGAC 
ATCTTGGCCA GGGGCTGCCC CACGGAACCA GTGACCCTGA CGCTATTCTC AGAGACCACC 
TTGCACCAGG CACAAAACCT CTTTAAGCTG TTGAACCTTC AGTCCCTCTT CGTGACATCG 
CGGGGCAGAG CTGTGGGCTG CGTGTCCTGG GTGGAGATGA AGAAAGCAAT TTCCAACCTG 
ACAAATCCGC CAGCTCCAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004061.3
CDS	81..2144
Translation	MEELVGLREGFSGDPVTLQELWGPCPHIRRAIQGGLEWLKQKVFRLGEDWYFLMTLGVLMALVSYAMNFAIGCVVRAHQWLYREIGDSHLLRYLSWTVYPVALVSFSSGFSQSITPSSGGSGIPELKTMLAGVILEDYLDIKNFGAKVVGLSCTLATGSTLFLGKVGPFVHLSVMIAAYLGRVRTTTIGEPENKSKQNEMLVAAAAVGVATVFAAPFSGVLFSIEVMSSHFSVRDYWRGFFAATCGAFIFRLLAVFNSEQETITSLYKTSFRVDVPFDLPEIFFFVALGGICGVLSCAYLFCQRTFLSFIKTNRYSSKLLATSKPVYSALATLLLASITYPPGVGHFLASRLSMKQHLDSLFDNHSWALMTQNSSPPWPEELDPQHLWWEWYHPRFTIFGTLAFFLVMKFWMLILATTIPMPAGYFMPIFILGAAIGRLLGEALAVAFPEGIVTGGVTNPIMPGGYALAGAAAFSGAVTHTISTALLAFELTGQIVHALPVLMAVLAANAIAQSCQPSFYDGTIIVKKLPYLPRILGRNIGSHHVRVEHFMNHSITTLAKDTPLEEVVKVVTSTDVTEYPLVESTESQILVGIVQRAQLVQALQAEPPSRAPGHQQCLQDILARGCPTEPVTLTLFSETTLHQAQNLFKLLNLQSLFVTSRGRAVGCVSWVEMKKAISNLTNPPAPK

Target ORF information:

RefSeq Version	NM_004070.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004070.3

ORF Insert Sequence:

ATGGAGGAGT TGGTGGGGCT GCGTGAGGGC TTCTCAGGGG ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCA CATCCGCCGA GCCATCCAAG GTGGCCTGGA GTGGCTAAAG 
CAGAAGGTGT TCCGCCTGGG AGAAGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTATGCCAT GAACTTTGCC ATCGGGTGTG TGGTCCGAGC ACACCAGTGG 
CTGTACAGGG AGATTGGGGA CAGCCACCTG CTCCGGTATC TTTCCTGGAC TGTGTACCCT 
GTGGCCCTCG TCTCTTTCTC CTCAGGCTTC TCCCAGAGCA TCACGCCCTC CTCTGGAGGT 
TCTGGAATCC CGGAGCTGAA GACCATGTTG GCGGGTGTGA TCTTGGAGGA CTACCTGGAT 
ATCAAGAACT TTGGGGCCAA GGTGGTGGGC CTCTCCTGCA CCCTGGCCAC CGGCAGCACC 
CTGTTCCTGG GCAAAGTGGG CCCTTTCGTG CACCTGTCTG TAATGATCGC TGCCTACCTG 
GGCCGTGTGC GCACCACGAC CATCGGGGAG CCTGAGAACA AGAGCAAGCA AAACGAAATG 
CTGGTGGCAG CGGCGGCAGT GGGCGTGGCC ACAGTCTTTG CAGCTCCCTT CAGCGGCGTC 
CTGTTCAGCA TCGAGGTCAT GTCTTCCCAC TTCTCTGTCC GGGATTACTG GAGGGGCTTC 
TTTGCGGCCA CCTGCGGGGC CTTCATATTC CGGCTCCTGG CAGTCTTCAA CAGCGAGCAG 
GAGACCATCA CCTCCCTCTA CAAGACCAGT TTCCGGGTGG ACGTTCCCTT CGACCTGCCT 
GAGATCTTCT TTTTTGTGGC GCTGGGTGGC ATCTGCGGCG TCCTGAGCTG TGCTTACCTC 
TTCTGTCAGC GAACCTTCCT CAGCTTCATC AAGACCAATC GGTACAGCTC CAAACTGCTG 
GCTACTAGCA AGCCTGTGTA CTCCGCTCTG GCCACCTTGC TTCTCGCCTC CATCACCTAC 
CCGCCTGGTG TGGGCCACTT CCTAGCTTCT CGGCTGTCCA TGAAGCAGCA TCTGGACTCG 
CTGTTCGACA ACCACTCCTG GGCGCTGATG ACCCAGAACT CCAGCCCACC CTGGCCCGAG 
GAGCTCGACC CCCAGCACCT TTGGTGGGAA TGGTACCACC CGCGGTTCAC CATCTTTGGG 
ACCCTTGCCT TCTTCCTGGT TATGAAGTTC TGGATGCTGA TTCTGGCCAC CACCATCCCC 
ATGCCTGCCG GGTACTTCAT GCCCATCTTT ATCCTTGGAG CTGCCATCGG GCGCCTCTTG 
GGAGAGGCTC TTGCCGTCGC CTTCCCTGAG GGCATTGTGA CTGGAGGGGT TACCAATCCC 
ATCATGCCCG GGGGGTATGC TCTGGCAGGG GCTGCAGCCT TCTCAGGGGC TGTGACCCAC 
ACCATCTCCA CGGCGCTGCT GGCCTTTGAG CTGACCGGCC AGATAGTGCA TGCACTGCCC 
GTGCTGATGG CGGTGCTGGC AGCCAACGCC ATTGCACAGA GCTGCCAGCC CTCCTTCTAT 
GATGGCACCA TCATTGTCAA GAAGCTGCCA TACCTGCCAC GGATTCTGGG CCGCAACATC 
GGCTCCCACC ATGTGAGGGT GGAGCACTTC ATGAACCACA GCATCACCAC ACTGGCCAAG 
GACACGCCGC TGGAGGAGGT GGTCAAGGTT GTGACCTCCA CAGACGTGAC CGAGTATCCC 
CTGGTGGAGA GCACAGAGTC CCAGATCCTG GTAGGCATCG TGCAGAGGGC CCAGCTGGTG 
CAGGCCCTCC AGGCTGAGCC TCCTTCCAGG GCTCCAGGAC ACCAGCAGTG TCTCCAGGAC 
ATCTTGGCCA GGGGCTGCCC CACGGAACCA GTGACCCTGA CGCTATTCTC AGAGACCACC 
TTGCACCAGG CACAAAACCT CTTTAAGCTG TTGAACCTTC AGTCCCTCTT CGTGACATCG 
CGGGGCAGAG CTGTGGGCTG CGTGTCCTGG GTGGAGATGA AGAAAGCAAT TTCCAACCTG 
ACAAATCCGC CAGCTCCAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCNKA ( NM_004070.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004061.3 Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19650
Clone ID Related Accession (Same CDS sequence)	NM_004070.3 , NM_004070.4
Accession Version	NM_004070.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	chloride channel protein ClC-Ka isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC053869.1 and Z30643.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004070.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290814.1, Z30643.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000331433.5/ ENSP00000332771.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGAGT TGGTGGGGCT GCGTGAGGGC TTCTCAGGGG ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCA CATCCGCCGA GCCATCCAAG GTGGCCTGGA GTGGCTAAAG 
CAGAAGGTGT TCCGCCTGGG AGAAGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTATGCCAT GAACTTTGCC ATCGGGTGTG TGGTCCGAGC ACACCAGTGG 
CTGTACAGGG AGATTGGGGA CAGCCACCTG CTCCGGTATC TTTCCTGGAC TGTGTACCCT 
GTGGCCCTCG TCTCTTTCTC CTCAGGCTTC TCCCAGAGCA TCACGCCCTC CTCTGGAGGT 
TCTGGAATCC CGGAGCTGAA GACCATGTTG GCGGGTGTGA TCTTGGAGGA CTACCTGGAT 
ATCAAGAACT TTGGGGCCAA GGTGGTGGGC CTCTCCTGCA CCCTGGCCAC CGGCAGCACC 
CTGTTCCTGG GCAAAGTGGG CCCTTTCGTG CACCTGTCTG TAATGATCGC TGCCTACCTG 
GGCCGTGTGC GCACCACGAC CATCGGGGAG CCTGAGAACA AGAGCAAGCA AAACGAAATG 
CTGGTGGCAG CGGCGGCAGT GGGCGTGGCC ACAGTCTTTG CAGCTCCCTT CAGCGGCGTC 
CTGTTCAGCA TCGAGGTCAT GTCTTCCCAC TTCTCTGTCC GGGATTACTG GAGGGGCTTC 
TTTGCGGCCA CCTGCGGGGC CTTCATATTC CGGCTCCTGG CAGTCTTCAA CAGCGAGCAG 
GAGACCATCA CCTCCCTCTA CAAGACCAGT TTCCGGGTGG ACGTTCCCTT CGACCTGCCT 
GAGATCTTCT TTTTTGTGGC GCTGGGTGGC ATCTGCGGCG TCCTGAGCTG TGCTTACCTC 
TTCTGTCAGC GAACCTTCCT CAGCTTCATC AAGACCAATC GGTACAGCTC CAAACTGCTG 
GCTACTAGCA AGCCTGTGTA CTCCGCTCTG GCCACCTTGC TTCTCGCCTC CATCACCTAC 
CCGCCTGGTG TGGGCCACTT CCTAGCTTCT CGGCTGTCCA TGAAGCAGCA TCTGGACTCG 
CTGTTCGACA ACCACTCCTG GGCGCTGATG ACCCAGAACT CCAGCCCACC CTGGCCCGAG 
GAGCTCGACC CCCAGCACCT TTGGTGGGAA TGGTACCACC CGCGGTTCAC CATCTTTGGG 
ACCCTTGCCT TCTTCCTGGT TATGAAGTTC TGGATGCTGA TTCTGGCCAC CACCATCCCC 
ATGCCTGCCG GGTACTTCAT GCCCATCTTT ATCCTTGGAG CTGCCATCGG GCGCCTCTTG 
GGAGAGGCTC TTGCCGTCGC CTTCCCTGAG GGCATTGTGA CTGGAGGGGT TACCAATCCC 
ATCATGCCCG GGGGGTATGC TCTGGCAGGG GCTGCAGCCT TCTCAGGGGC TGTGACCCAC 
ACCATCTCCA CGGCGCTGCT GGCCTTTGAG CTGACCGGCC AGATAGTGCA TGCACTGCCC 
GTGCTGATGG CGGTGCTGGC AGCCAACGCC ATTGCACAGA GCTGCCAGCC CTCCTTCTAT 
GATGGCACCA TCATTGTCAA GAAGCTGCCA TACCTGCCAC GGATTCTGGG CCGCAACATC 
GGCTCCCACC ATGTGAGGGT GGAGCACTTC ATGAACCACA GCATCACCAC ACTGGCCAAG 
GACACGCCGC TGGAGGAGGT GGTCAAGGTT GTGACCTCCA CAGACGTGAC CGAGTATCCC 
CTGGTGGAGA GCACAGAGTC CCAGATCCTG GTAGGCATCG TGCAGAGGGC CCAGCTGGTG 
CAGGCCCTCC AGGCTGAGCC TCCTTCCAGG GCTCCAGGAC ACCAGCAGTG TCTCCAGGAC 
ATCTTGGCCA GGGGCTGCCC CACGGAACCA GTGACCCTGA CGCTATTCTC AGAGACCACC 
TTGCACCAGG CACAAAACCT CTTTAAGCTG TTGAACCTTC AGTCCCTCTT CGTGACATCG 
CGGGGCAGAG CTGTGGGCTG CGTGTCCTGG GTGGAGATGA AGAAAGCAAT TTCCAACCTG 
ACAAATCCGC CAGCTCCAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004061.3
CDS	36..2099
Translation	MEELVGLREGFSGDPVTLQELWGPCPHIRRAIQGGLEWLKQKVFRLGEDWYFLMTLGVLMALVSYAMNFAIGCVVRAHQWLYREIGDSHLLRYLSWTVYPVALVSFSSGFSQSITPSSGGSGIPELKTMLAGVILEDYLDIKNFGAKVVGLSCTLATGSTLFLGKVGPFVHLSVMIAAYLGRVRTTTIGEPENKSKQNEMLVAAAAVGVATVFAAPFSGVLFSIEVMSSHFSVRDYWRGFFAATCGAFIFRLLAVFNSEQETITSLYKTSFRVDVPFDLPEIFFFVALGGICGVLSCAYLFCQRTFLSFIKTNRYSSKLLATSKPVYSALATLLLASITYPPGVGHFLASRLSMKQHLDSLFDNHSWALMTQNSSPPWPEELDPQHLWWEWYHPRFTIFGTLAFFLVMKFWMLILATTIPMPAGYFMPIFILGAAIGRLLGEALAVAFPEGIVTGGVTNPIMPGGYALAGAAAFSGAVTHTISTALLAFELTGQIVHALPVLMAVLAANAIAQSCQPSFYDGTIIVKKLPYLPRILGRNIGSHHVRVEHFMNHSITTLAKDTPLEEVVKVVTSTDVTEYPLVESTESQILVGIVQRAQLVQALQAEPPSRAPGHQQCLQDILARGCPTEPVTLTLFSETTLHQAQNLFKLLNLQSLFVTSRGRAVGCVSWVEMKKAISNLTNPPAPK

Target ORF information:

RefSeq Version	NM_004070.4
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004070.4

ORF Insert Sequence:

ATGGAGGAGT TGGTGGGGCT GCGTGAGGGC TTCTCAGGGG ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCA CATCCGCCGA GCCATCCAAG GTGGCCTGGA GTGGCTAAAG 
CAGAAGGTGT TCCGCCTGGG AGAAGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTATGCCAT GAACTTTGCC ATCGGGTGTG TGGTCCGAGC ACACCAGTGG 
CTGTACAGGG AGATTGGGGA CAGCCACCTG CTCCGGTATC TTTCCTGGAC TGTGTACCCT 
GTGGCCCTCG TCTCTTTCTC CTCAGGCTTC TCCCAGAGCA TCACGCCCTC CTCTGGAGGT 
TCTGGAATCC CGGAGCTGAA GACCATGTTG GCGGGTGTGA TCTTGGAGGA CTACCTGGAT 
ATCAAGAACT TTGGGGCCAA GGTGGTGGGC CTCTCCTGCA CCCTGGCCAC CGGCAGCACC 
CTGTTCCTGG GCAAAGTGGG CCCTTTCGTG CACCTGTCTG TAATGATCGC TGCCTACCTG 
GGCCGTGTGC GCACCACGAC CATCGGGGAG CCTGAGAACA AGAGCAAGCA AAACGAAATG 
CTGGTGGCAG CGGCGGCAGT GGGCGTGGCC ACAGTCTTTG CAGCTCCCTT CAGCGGCGTC 
CTGTTCAGCA TCGAGGTCAT GTCTTCCCAC TTCTCTGTCC GGGATTACTG GAGGGGCTTC 
TTTGCGGCCA CCTGCGGGGC CTTCATATTC CGGCTCCTGG CAGTCTTCAA CAGCGAGCAG 
GAGACCATCA CCTCCCTCTA CAAGACCAGT TTCCGGGTGG ACGTTCCCTT CGACCTGCCT 
GAGATCTTCT TTTTTGTGGC GCTGGGTGGC ATCTGCGGCG TCCTGAGCTG TGCTTACCTC 
TTCTGTCAGC GAACCTTCCT CAGCTTCATC AAGACCAATC GGTACAGCTC CAAACTGCTG 
GCTACTAGCA AGCCTGTGTA CTCCGCTCTG GCCACCTTGC TTCTCGCCTC CATCACCTAC 
CCGCCTGGTG TGGGCCACTT CCTAGCTTCT CGGCTGTCCA TGAAGCAGCA TCTGGACTCG 
CTGTTCGACA ACCACTCCTG GGCGCTGATG ACCCAGAACT CCAGCCCACC CTGGCCCGAG 
GAGCTCGACC CCCAGCACCT TTGGTGGGAA TGGTACCACC CGCGGTTCAC CATCTTTGGG 
ACCCTTGCCT TCTTCCTGGT TATGAAGTTC TGGATGCTGA TTCTGGCCAC CACCATCCCC 
ATGCCTGCCG GGTACTTCAT GCCCATCTTT ATCCTTGGAG CTGCCATCGG GCGCCTCTTG 
GGAGAGGCTC TTGCCGTCGC CTTCCCTGAG GGCATTGTGA CTGGAGGGGT TACCAATCCC 
ATCATGCCCG GGGGGTATGC TCTGGCAGGG GCTGCAGCCT TCTCAGGGGC TGTGACCCAC 
ACCATCTCCA CGGCGCTGCT GGCCTTTGAG CTGACCGGCC AGATAGTGCA TGCACTGCCC 
GTGCTGATGG CGGTGCTGGC AGCCAACGCC ATTGCACAGA GCTGCCAGCC CTCCTTCTAT 
GATGGCACCA TCATTGTCAA GAAGCTGCCA TACCTGCCAC GGATTCTGGG CCGCAACATC 
GGCTCCCACC ATGTGAGGGT GGAGCACTTC ATGAACCACA GCATCACCAC ACTGGCCAAG 
GACACGCCGC TGGAGGAGGT GGTCAAGGTT GTGACCTCCA CAGACGTGAC CGAGTATCCC 
CTGGTGGAGA GCACAGAGTC CCAGATCCTG GTAGGCATCG TGCAGAGGGC CCAGCTGGTG 
CAGGCCCTCC AGGCTGAGCC TCCTTCCAGG GCTCCAGGAC ACCAGCAGTG TCTCCAGGAC 
ATCTTGGCCA GGGGCTGCCC CACGGAACCA GTGACCCTGA CGCTATTCTC AGAGACCACC 
TTGCACCAGG CACAAAACCT CTTTAAGCTG TTGAACCTTC AGTCCCTCTT CGTGACATCG 
CGGGGCAGAG CTGTGGGCTG CGTGTCCTGG GTGGAGATGA AGAAAGCAAT TTCCAACCTG 
ACAAATCCGC CAGCTCCAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCNKA ( NM_001257139.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244068.1 Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12112
Clone ID Related Accession (Same CDS sequence)	NM_001257139.1 , NM_001257139.2
Accession Version	NM_001257139.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	chloride channel protein ClC-Ka isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355994.11, AK298285.1 and AI821546.1. On Jul 26, 2012 this sequence version replaced XM_003846620.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting protein is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298285.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162328, SAMN03465407 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGT TGGTGGGGCT GCGTGAGGGC TTCTCAGGGG ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCA CATCCGCCGA GCCATCCAAG GTGGCCTGGA GTGGCTAAAG 
CAGAAGGTGT TCCGCCTGGG AGAAGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTATGCCAT GAACTTTGCC ATCGGGTGTG TGGTCCGAGG TTCTGGAATC 
CCGGAGCTGA AGACCATGTT GGCGGGTGTG ATCTTGGAGG ACTACCTGGA TATCAAGAAC 
TTTGGGGCCA AGGTGGTGGG CCTCTCCTGC ACCCTGGCCA CCGGCAGCAC CCTGTTCCTG 
GGCAAAGTGG GCCCTTTCGT GCACCTGTCT GTAATGATCG CTGCCTACCT GGGCCGTGTG 
CGCACCACGA CCATCGGGGA GCCTGAGAAC AAGAGCAAGC AAAACGAAAT GCTGGTGGCA 
GCGGCGGCAG TGGGCGTGGC CACAGTCTTT GCAGCTCCCT TCAGCGGCGT CCTGTTCAGC 
ATCGAGGTCA TGTCTTCCCA CTTCTCTGTC CGGGATTACT GGAGGGGCTT CTTTGCGGCC 
ACCTGCGGGG CCTTCATATT CCGGCTCCTG GCAGTCTTCA ACAGCGAGCA GGAGACCATC 
ACCTCCCTCT ACAAGACCAG TTTCCGGGTG GACGTTCCCT TCGACCTGCC TGAGATCTTC 
TTTTTTGTGG CGCTGGGTGG CATCTGCGGC GTCCTGAGCT GTGCTTACCT CTTCTGTCAG 
CGAACCTTCC TCAGCTTCAT CAAGACCAAT CGGTACAGCT CCAAACTGCT GGCTACTAGC 
AAGCCTGTGT ACTCCGCTCT GGCCACCTTG CTTCTCGCCT CCATCACCTA CCCGCCTGGT 
GTGGGCCACT TCCTAGCTTC TCGGCTGTCC ATGAAGCAGC ATCTGGACTC GCTGTTCGAC 
AACCACTCCT GGGCGCTGAT GACCCAGAAC TCCAGCCCAC CCTGGCCCGA GGAGCTCGAC 
CCCCAGCACC TTTGGTGGGA ATGGTACCAC CCGCGGTTCA CCATCTTTGG GACCCTTGCC 
TTCTTCCTGG TTATGAAGTT CTGGATGCTG ATTCTGGCCA CCACCATCCC CATGCCTGCC 
GGGTACTTCA TGCCCATCTT TATCCTTGGA GCTGCCATCG GGCGCCTCTT GGGAGAGGCT 
CTTGCCGTCG CCTTCCCTGA GGGCATTGTG ACTGGAGGGG TTACCAATCC CATCATGCCC 
GGGGGGTATG CTCTGGCAGG GGCTGCAGCC TTCTCAGGGG CTGTGACCCA CACCATCTCC 
ACGGCGCTGC TGGCCTTTGA GCTGACCGGC CAGATAGTGC ATGCACTGCC CGTGCTGATG 
GCGGTGCTGG CAGCCAACGC CATTGCACAG AGCTGCCAGC CCTCCTTCTA TGATGGCACC 
ATCATTGTCA AGAAGCTGCC ATACCTGCCA CGGATTCTGG GCCGCAACAT CGGCTCCCAC 
CATGTGAGGG TGGAGCACTT CATGAACCAC AGCATCACCA CACTGGCCAA GGACACGCCG 
CTGGAGGAGG TGGTCAAGGT TGTGACCTCC ACAGACGTGA CCGAGTATCC CCTGGTGGAG 
AGCACAGAGT CCCAGATCCT GGTAGGCATC GTGCAGAGGG CCCAGCTGGT GCAGGCCCTC 
CAGGCTGAGC CTCCTTCCAG GGCTCCAGGA CACCAGCAGT GTCTCCAGGA CATCTTGGCC 
AGGGGCTGCC CCACGGAACC AGTGACCCTG ACGCTATTCT CAGAGACCAC CTTGCACCAG 
GCACAAAACC TCTTTAAGCT GTTGAACCTT CAGTCCCTCT TCGTGACATC GCGGGGCAGA 
GCTGTGGGCT GCGTGTCCTG GGTGGAGATG AAGAAAGCAA TTTCCAACCT GACAAATCCG 
CCAGCTCCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244068.1
CDS	81..2015
Translation	MEELVGLREGFSGDPVTLQELWGPCPHIRRAIQGGLEWLKQKVFRLGEDWYFLMTLGVLMALVSYAMNFAIGCVVRGSGIPELKTMLAGVILEDYLDIKNFGAKVVGLSCTLATGSTLFLGKVGPFVHLSVMIAAYLGRVRTTTIGEPENKSKQNEMLVAAAAVGVATVFAAPFSGVLFSIEVMSSHFSVRDYWRGFFAATCGAFIFRLLAVFNSEQETITSLYKTSFRVDVPFDLPEIFFFVALGGICGVLSCAYLFCQRTFLSFIKTNRYSSKLLATSKPVYSALATLLLASITYPPGVGHFLASRLSMKQHLDSLFDNHSWALMTQNSSPPWPEELDPQHLWWEWYHPRFTIFGTLAFFLVMKFWMLILATTIPMPAGYFMPIFILGAAIGRLLGEALAVAFPEGIVTGGVTNPIMPGGYALAGAAAFSGAVTHTISTALLAFELTGQIVHALPVLMAVLAANAIAQSCQPSFYDGTIIVKKLPYLPRILGRNIGSHHVRVEHFMNHSITTLAKDTPLEEVVKVVTSTDVTEYPLVESTESQILVGIVQRAQLVQALQAEPPSRAPGHQQCLQDILARGCPTEPVTLTLFSETTLHQAQNLFKLLNLQSLFVTSRGRAVGCVSWVEMKKAISNLTNPPAPK

Target ORF information:

RefSeq Version	NM_001257139.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257139.1

ORF Insert Sequence:

ATGGAGGAGT TGGTGGGGCT GCGTGAGGGC TTCTCAGGGG ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCA CATCCGCCGA GCCATCCAAG GTGGCCTGGA GTGGCTAAAG 
CAGAAGGTGT TCCGCCTGGG AGAAGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTATGCCAT GAACTTTGCC ATCGGGTGTG TGGTCCGAGG TTCTGGAATC 
CCGGAGCTGA AGACCATGTT GGCGGGTGTG ATCTTGGAGG ACTACCTGGA TATCAAGAAC 
TTTGGGGCCA AGGTGGTGGG CCTCTCCTGC ACCCTGGCCA CCGGCAGCAC CCTGTTCCTG 
GGCAAAGTGG GCCCTTTCGT GCACCTGTCT GTAATGATCG CTGCCTACCT GGGCCGTGTG 
CGCACCACGA CCATCGGGGA GCCTGAGAAC AAGAGCAAGC AAAACGAAAT GCTGGTGGCA 
GCGGCGGCAG TGGGCGTGGC CACAGTCTTT GCAGCTCCCT TCAGCGGCGT CCTGTTCAGC 
ATCGAGGTCA TGTCTTCCCA CTTCTCTGTC CGGGATTACT GGAGGGGCTT CTTTGCGGCC 
ACCTGCGGGG CCTTCATATT CCGGCTCCTG GCAGTCTTCA ACAGCGAGCA GGAGACCATC 
ACCTCCCTCT ACAAGACCAG TTTCCGGGTG GACGTTCCCT TCGACCTGCC TGAGATCTTC 
TTTTTTGTGG CGCTGGGTGG CATCTGCGGC GTCCTGAGCT GTGCTTACCT CTTCTGTCAG 
CGAACCTTCC TCAGCTTCAT CAAGACCAAT CGGTACAGCT CCAAACTGCT GGCTACTAGC 
AAGCCTGTGT ACTCCGCTCT GGCCACCTTG CTTCTCGCCT CCATCACCTA CCCGCCTGGT 
GTGGGCCACT TCCTAGCTTC TCGGCTGTCC ATGAAGCAGC ATCTGGACTC GCTGTTCGAC 
AACCACTCCT GGGCGCTGAT GACCCAGAAC TCCAGCCCAC CCTGGCCCGA GGAGCTCGAC 
CCCCAGCACC TTTGGTGGGA ATGGTACCAC CCGCGGTTCA CCATCTTTGG GACCCTTGCC 
TTCTTCCTGG TTATGAAGTT CTGGATGCTG ATTCTGGCCA CCACCATCCC CATGCCTGCC 
GGGTACTTCA TGCCCATCTT TATCCTTGGA GCTGCCATCG GGCGCCTCTT GGGAGAGGCT 
CTTGCCGTCG CCTTCCCTGA GGGCATTGTG ACTGGAGGGG TTACCAATCC CATCATGCCC 
GGGGGGTATG CTCTGGCAGG GGCTGCAGCC TTCTCAGGGG CTGTGACCCA CACCATCTCC 
ACGGCGCTGC TGGCCTTTGA GCTGACCGGC CAGATAGTGC ATGCACTGCC CGTGCTGATG 
GCGGTGCTGG CAGCCAACGC CATTGCACAG AGCTGCCAGC CCTCCTTCTA TGATGGCACC 
ATCATTGTCA AGAAGCTGCC ATACCTGCCA CGGATTCTGG GCCGCAACAT CGGCTCCCAC 
CATGTGAGGG TGGAGCACTT CATGAACCAC AGCATCACCA CACTGGCCAA GGACACGCCG 
CTGGAGGAGG TGGTCAAGGT TGTGACCTCC ACAGACGTGA CCGAGTATCC CCTGGTGGAG 
AGCACAGAGT CCCAGATCCT GGTAGGCATC GTGCAGAGGG CCCAGCTGGT GCAGGCCCTC 
CAGGCTGAGC CTCCTTCCAG GGCTCCAGGA CACCAGCAGT GTCTCCAGGA CATCTTGGCC 
AGGGGCTGCC CCACGGAACC AGTGACCCTG ACGCTATTCT CAGAGACCAC CTTGCACCAG 
GCACAAAACC TCTTTAAGCT GTTGAACCTT CAGTCCCTCT TCGTGACATC GCGGGGCAGA 
GCTGTGGGCT GCGTGTCCTG GGTGGAGATG AAGAAAGCAA TTTCCAACCT GACAAATCCG 
CCAGCTCCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCNKA ( NM_001257139.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244068.1 Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12112
Clone ID Related Accession (Same CDS sequence)	NM_001257139.1 , NM_001257139.2
Accession Version	NM_001257139.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	chloride channel protein ClC-Ka isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK298285.1, AL355994.11 and AI821546.1. On May 31, 2019 this sequence version replaced NM_001257139.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting protein is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298285.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162328, SAMN03465407 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGT TGGTGGGGCT GCGTGAGGGC TTCTCAGGGG ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCA CATCCGCCGA GCCATCCAAG GTGGCCTGGA GTGGCTAAAG 
CAGAAGGTGT TCCGCCTGGG AGAAGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTATGCCAT GAACTTTGCC ATCGGGTGTG TGGTCCGAGG TTCTGGAATC 
CCGGAGCTGA AGACCATGTT GGCGGGTGTG ATCTTGGAGG ACTACCTGGA TATCAAGAAC 
TTTGGGGCCA AGGTGGTGGG CCTCTCCTGC ACCCTGGCCA CCGGCAGCAC CCTGTTCCTG 
GGCAAAGTGG GCCCTTTCGT GCACCTGTCT GTAATGATCG CTGCCTACCT GGGCCGTGTG 
CGCACCACGA CCATCGGGGA GCCTGAGAAC AAGAGCAAGC AAAACGAAAT GCTGGTGGCA 
GCGGCGGCAG TGGGCGTGGC CACAGTCTTT GCAGCTCCCT TCAGCGGCGT CCTGTTCAGC 
ATCGAGGTCA TGTCTTCCCA CTTCTCTGTC CGGGATTACT GGAGGGGCTT CTTTGCGGCC 
ACCTGCGGGG CCTTCATATT CCGGCTCCTG GCAGTCTTCA ACAGCGAGCA GGAGACCATC 
ACCTCCCTCT ACAAGACCAG TTTCCGGGTG GACGTTCCCT TCGACCTGCC TGAGATCTTC 
TTTTTTGTGG CGCTGGGTGG CATCTGCGGC GTCCTGAGCT GTGCTTACCT CTTCTGTCAG 
CGAACCTTCC TCAGCTTCAT CAAGACCAAT CGGTACAGCT CCAAACTGCT GGCTACTAGC 
AAGCCTGTGT ACTCCGCTCT GGCCACCTTG CTTCTCGCCT CCATCACCTA CCCGCCTGGT 
GTGGGCCACT TCCTAGCTTC TCGGCTGTCC ATGAAGCAGC ATCTGGACTC GCTGTTCGAC 
AACCACTCCT GGGCGCTGAT GACCCAGAAC TCCAGCCCAC CCTGGCCCGA GGAGCTCGAC 
CCCCAGCACC TTTGGTGGGA ATGGTACCAC CCGCGGTTCA CCATCTTTGG GACCCTTGCC 
TTCTTCCTGG TTATGAAGTT CTGGATGCTG ATTCTGGCCA CCACCATCCC CATGCCTGCC 
GGGTACTTCA TGCCCATCTT TATCCTTGGA GCTGCCATCG GGCGCCTCTT GGGAGAGGCT 
CTTGCCGTCG CCTTCCCTGA GGGCATTGTG ACTGGAGGGG TTACCAATCC CATCATGCCC 
GGGGGGTATG CTCTGGCAGG GGCTGCAGCC TTCTCAGGGG CTGTGACCCA CACCATCTCC 
ACGGCGCTGC TGGCCTTTGA GCTGACCGGC CAGATAGTGC ATGCACTGCC CGTGCTGATG 
GCGGTGCTGG CAGCCAACGC CATTGCACAG AGCTGCCAGC CCTCCTTCTA TGATGGCACC 
ATCATTGTCA AGAAGCTGCC ATACCTGCCA CGGATTCTGG GCCGCAACAT CGGCTCCCAC 
CATGTGAGGG TGGAGCACTT CATGAACCAC AGCATCACCA CACTGGCCAA GGACACGCCG 
CTGGAGGAGG TGGTCAAGGT TGTGACCTCC ACAGACGTGA CCGAGTATCC CCTGGTGGAG 
AGCACAGAGT CCCAGATCCT GGTAGGCATC GTGCAGAGGG CCCAGCTGGT GCAGGCCCTC 
CAGGCTGAGC CTCCTTCCAG GGCTCCAGGA CACCAGCAGT GTCTCCAGGA CATCTTGGCC 
AGGGGCTGCC CCACGGAACC AGTGACCCTG ACGCTATTCT CAGAGACCAC CTTGCACCAG 
GCACAAAACC TCTTTAAGCT GTTGAACCTT CAGTCCCTCT TCGTGACATC GCGGGGCAGA 
GCTGTGGGCT GCGTGTCCTG GGTGGAGATG AAGAAAGCAA TTTCCAACCT GACAAATCCG 
CCAGCTCCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244068.1
CDS	36..1970
Translation	MEELVGLREGFSGDPVTLQELWGPCPHIRRAIQGGLEWLKQKVFRLGEDWYFLMTLGVLMALVSYAMNFAIGCVVRGSGIPELKTMLAGVILEDYLDIKNFGAKVVGLSCTLATGSTLFLGKVGPFVHLSVMIAAYLGRVRTTTIGEPENKSKQNEMLVAAAAVGVATVFAAPFSGVLFSIEVMSSHFSVRDYWRGFFAATCGAFIFRLLAVFNSEQETITSLYKTSFRVDVPFDLPEIFFFVALGGICGVLSCAYLFCQRTFLSFIKTNRYSSKLLATSKPVYSALATLLLASITYPPGVGHFLASRLSMKQHLDSLFDNHSWALMTQNSSPPWPEELDPQHLWWEWYHPRFTIFGTLAFFLVMKFWMLILATTIPMPAGYFMPIFILGAAIGRLLGEALAVAFPEGIVTGGVTNPIMPGGYALAGAAAFSGAVTHTISTALLAFELTGQIVHALPVLMAVLAANAIAQSCQPSFYDGTIIVKKLPYLPRILGRNIGSHHVRVEHFMNHSITTLAKDTPLEEVVKVVTSTDVTEYPLVESTESQILVGIVQRAQLVQALQAEPPSRAPGHQQCLQDILARGCPTEPVTLTLFSETTLHQAQNLFKLLNLQSLFVTSRGRAVGCVSWVEMKKAISNLTNPPAPK

Target ORF information:

RefSeq Version	NM_001257139.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257139.2

ORF Insert Sequence:

ATGGAGGAGT TGGTGGGGCT GCGTGAGGGC TTCTCAGGGG ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCA CATCCGCCGA GCCATCCAAG GTGGCCTGGA GTGGCTAAAG 
CAGAAGGTGT TCCGCCTGGG AGAAGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTATGCCAT GAACTTTGCC ATCGGGTGTG TGGTCCGAGG TTCTGGAATC 
CCGGAGCTGA AGACCATGTT GGCGGGTGTG ATCTTGGAGG ACTACCTGGA TATCAAGAAC 
TTTGGGGCCA AGGTGGTGGG CCTCTCCTGC ACCCTGGCCA CCGGCAGCAC CCTGTTCCTG 
GGCAAAGTGG GCCCTTTCGT GCACCTGTCT GTAATGATCG CTGCCTACCT GGGCCGTGTG 
CGCACCACGA CCATCGGGGA GCCTGAGAAC AAGAGCAAGC AAAACGAAAT GCTGGTGGCA 
GCGGCGGCAG TGGGCGTGGC CACAGTCTTT GCAGCTCCCT TCAGCGGCGT CCTGTTCAGC 
ATCGAGGTCA TGTCTTCCCA CTTCTCTGTC CGGGATTACT GGAGGGGCTT CTTTGCGGCC 
ACCTGCGGGG CCTTCATATT CCGGCTCCTG GCAGTCTTCA ACAGCGAGCA GGAGACCATC 
ACCTCCCTCT ACAAGACCAG TTTCCGGGTG GACGTTCCCT TCGACCTGCC TGAGATCTTC 
TTTTTTGTGG CGCTGGGTGG CATCTGCGGC GTCCTGAGCT GTGCTTACCT CTTCTGTCAG 
CGAACCTTCC TCAGCTTCAT CAAGACCAAT CGGTACAGCT CCAAACTGCT GGCTACTAGC 
AAGCCTGTGT ACTCCGCTCT GGCCACCTTG CTTCTCGCCT CCATCACCTA CCCGCCTGGT 
GTGGGCCACT TCCTAGCTTC TCGGCTGTCC ATGAAGCAGC ATCTGGACTC GCTGTTCGAC 
AACCACTCCT GGGCGCTGAT GACCCAGAAC TCCAGCCCAC CCTGGCCCGA GGAGCTCGAC 
CCCCAGCACC TTTGGTGGGA ATGGTACCAC CCGCGGTTCA CCATCTTTGG GACCCTTGCC 
TTCTTCCTGG TTATGAAGTT CTGGATGCTG ATTCTGGCCA CCACCATCCC CATGCCTGCC 
GGGTACTTCA TGCCCATCTT TATCCTTGGA GCTGCCATCG GGCGCCTCTT GGGAGAGGCT 
CTTGCCGTCG CCTTCCCTGA GGGCATTGTG ACTGGAGGGG TTACCAATCC CATCATGCCC 
GGGGGGTATG CTCTGGCAGG GGCTGCAGCC TTCTCAGGGG CTGTGACCCA CACCATCTCC 
ACGGCGCTGC TGGCCTTTGA GCTGACCGGC CAGATAGTGC ATGCACTGCC CGTGCTGATG 
GCGGTGCTGG CAGCCAACGC CATTGCACAG AGCTGCCAGC CCTCCTTCTA TGATGGCACC 
ATCATTGTCA AGAAGCTGCC ATACCTGCCA CGGATTCTGG GCCGCAACAT CGGCTCCCAC 
CATGTGAGGG TGGAGCACTT CATGAACCAC AGCATCACCA CACTGGCCAA GGACACGCCG 
CTGGAGGAGG TGGTCAAGGT TGTGACCTCC ACAGACGTGA CCGAGTATCC CCTGGTGGAG 
AGCACAGAGT CCCAGATCCT GGTAGGCATC GTGCAGAGGG CCCAGCTGGT GCAGGCCCTC 
CAGGCTGAGC CTCCTTCCAG GGCTCCAGGA CACCAGCAGT GTCTCCAGGA CATCTTGGCC 
AGGGGCTGCC CCACGGAACC AGTGACCCTG ACGCTATTCT CAGAGACCAC CTTGCACCAG 
GCACAAAACC TCTTTAAGCT GTTGAACCTT CAGTCCCTCT TCGTGACATC GCGGGGCAGA 
GCTGTGGGCT GCGTGTCCTG GGTGGAGATG AAGAAAGCAA TTTCCAACCT GACAAATCCG 
CCAGCTCCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCNKA ( NM_001042704.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001036169.1 Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu30105
Clone ID Related Accession (Same CDS sequence)	NM_001042704.1 , NM_001042704.2
Accession Version	NM_001042704.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Homo sapiens(human)
Product	chloride channel protein ClC-Ka isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC053869.1 and AK225550.1. On Nov 2, 2012 this sequence version replaced XM_003960902.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a protein (isoform 2) that is one amino acid shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225550.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGT TGGTGGGGCT GCGTGAGGGC TTCTCAGGGG ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCA CATCCGCCGA GCCATCCAAG GTGGCCTGGA GTGGCTAAAG 
CAGAAGGTGT TCCGCCTGGG AGAAGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTATGCCAT GAACTTTGCC ATCGGGTGTG TGGTCCGAGC ACACCAGTGG 
CTGTACAGGG AGATTGGGGA CAGCCACCTG CTCCGGTATC TTTCCTGGAC TGTGTACCCT 
GTGGCCCTCG TCTCTTTCTC CTCAGGCTTC TCCCAGAGCA TCACGCCCTC CTCTGGAGGT 
TCTGGAATCC CGGAGCTGAA GACCATGTTG GCGGGTGTGA TCTTGGAGGA CTACCTGGAT 
ATCAAGAACT TTGGGGCCAA GGTGGTGGGC CTCTCCTGCA CCCTGGCCAC CGGCAGCACC 
CTGTTCCTGG GCAAAGTGGG CCCTTTCGTG CACCTGTCTG TAATGATCGC TGCCTACCTG 
GGCCGTGTGC GCACCACGAC CATCGGGGAG CCTGAGAACA AGAGCAAGCA AAACGAAATG 
CTGGTGGCAG CGGCGGCAGT GGGCGTGGCC ACAGTCTTTG CAGCTCCCTT CAGCGGCGTC 
CTGTTCAGCA TCGAGGTCAT GTCTTCCCAC TTCTCTGTCC GGGATTACTG GAGGGGCTTC 
TTTGCGGCCA CCTGCGGGGC CTTCATATTC CGGCTCCTGG CAGTCTTCAA CAGCGAGCAG 
GAGACCATCA CCTCCCTCTA CAAGACCAGT TTCCGGGTGG ACGTTCCCTT CGACCTGCCT 
GAGATCTTCT TTTTTGTGGC GCTGGGTGGC ATCTGCGGCG TCCTGAGCTG TGCTTACCTC 
TTCTGTCAGC GAACCTTCCT CAGCTTCATC AAGACCAATC GGTACAGCTC CAAACTGCTG 
GCTACTAGCA AGCCTGTGTA CTCCGCTCTG GCCACCTTGC TTCTCGCCTC CATCACCTAC 
CCGCCTGGTG TGGGCCACTT CCTAGCTTCT CGGCTGTCCA TGAAGCAGCA TCTGGACTCG 
CTGTTCGACA ACCACTCCTG GGCGCTGATG ACCCAGAACT CCAGCCCACC CTGGCCCGAG 
GAGCTCGACC CCCAGCACCT TTGGTGGGAA TGGTACCACC CGCGGTTCAC CATCTTTGGG 
ACCCTTGCCT TCTTCCTGGT TATGAAGTTC TGGATGCTGA TTCTGGCCAC CACCATCCCC 
ATGCCTGCCG GGTACTTCAT GCCCATCTTT ATCCTTGGAG CTGCCATCGG GCGCCTCTTG 
GGAGAGGCTC TTGCCGTCGC CTTCCCTGAG GGCATTGTGA CTGGAGGGGT TACCAATCCC 
ATCATGCCCG GGGGGTATGC TCTGGCAGGG GCTGCAGCCT TCTCAGGGGC TGTGACCCAC 
ACCATCTCCA CGGCGCTGCT GGCCTTTGAG CTGACCGGCC AGATAGTGCA TGCACTGCCC 
GTGCTGATGG CGGTGCTGGC AGCCAACGCC ATTGCACAGA GCTGCCAGCC CTCCTTCTAT 
GATGGCACCA TCATTGTCAA GAAGCTGCCA TACCTGCCAC GGATTCTGGG CCGCAACATC 
GGCTCCCACC ATGTGAGGGT GGAGCACTTC ATGAACCACA GCATCACCAC ACTGGCCAAG 
GACACGCCGC TGGAGGAGGT GGTCAAGGTT GTGACCTCCA CAGACGTGAC CGAGTATCCC 
CTGGTGGAGA GCACAGAGTC CCAGATCCTG GTAGGCATCG TGCAGAGGGC CCAGCTGGTG 
CAGGCCCTCC AGGCTGAGCC TCCTTCCAGG GCTCCAGGAC ACCAGTGTCT CCAGGACATC 
TTGGCCAGGG GCTGCCCCAC GGAACCAGTG ACCCTGACGC TATTCTCAGA GACCACCTTG 
CACCAGGCAC AAAACCTCTT TAAGCTGTTG AACCTTCAGT CCCTCTTCGT GACATCGCGG 
GGCAGAGCTG TGGGCTGCGT GTCCTGGGTG GAGATGAAGA AAGCAATTTC CAACCTGACA 
AATCCGCCAG CTCCAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036169.1
CDS	81..2141
Translation	MEELVGLREGFSGDPVTLQELWGPCPHIRRAIQGGLEWLKQKVFRLGEDWYFLMTLGVLMALVSYAMNFAIGCVVRAHQWLYREIGDSHLLRYLSWTVYPVALVSFSSGFSQSITPSSGGSGIPELKTMLAGVILEDYLDIKNFGAKVVGLSCTLATGSTLFLGKVGPFVHLSVMIAAYLGRVRTTTIGEPENKSKQNEMLVAAAAVGVATVFAAPFSGVLFSIEVMSSHFSVRDYWRGFFAATCGAFIFRLLAVFNSEQETITSLYKTSFRVDVPFDLPEIFFFVALGGICGVLSCAYLFCQRTFLSFIKTNRYSSKLLATSKPVYSALATLLLASITYPPGVGHFLASRLSMKQHLDSLFDNHSWALMTQNSSPPWPEELDPQHLWWEWYHPRFTIFGTLAFFLVMKFWMLILATTIPMPAGYFMPIFILGAAIGRLLGEALAVAFPEGIVTGGVTNPIMPGGYALAGAAAFSGAVTHTISTALLAFELTGQIVHALPVLMAVLAANAIAQSCQPSFYDGTIIVKKLPYLPRILGRNIGSHHVRVEHFMNHSITTLAKDTPLEEVVKVVTSTDVTEYPLVESTESQILVGIVQRAQLVQALQAEPPSRAPGHQCLQDILARGCPTEPVTLTLFSETTLHQAQNLFKLLNLQSLFVTSRGRAVGCVSWVEMKKAISNLTNPPAPK

Target ORF information:

RefSeq Version	NM_001042704.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042704.1

ORF Insert Sequence:

ATGGAGGAGT TGGTGGGGCT GCGTGAGGGC TTCTCAGGGG ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCA CATCCGCCGA GCCATCCAAG GTGGCCTGGA GTGGCTAAAG 
CAGAAGGTGT TCCGCCTGGG AGAAGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTATGCCAT GAACTTTGCC ATCGGGTGTG TGGTCCGAGC ACACCAGTGG 
CTGTACAGGG AGATTGGGGA CAGCCACCTG CTCCGGTATC TTTCCTGGAC TGTGTACCCT 
GTGGCCCTCG TCTCTTTCTC CTCAGGCTTC TCCCAGAGCA TCACGCCCTC CTCTGGAGGT 
TCTGGAATCC CGGAGCTGAA GACCATGTTG GCGGGTGTGA TCTTGGAGGA CTACCTGGAT 
ATCAAGAACT TTGGGGCCAA GGTGGTGGGC CTCTCCTGCA CCCTGGCCAC CGGCAGCACC 
CTGTTCCTGG GCAAAGTGGG CCCTTTCGTG CACCTGTCTG TAATGATCGC TGCCTACCTG 
GGCCGTGTGC GCACCACGAC CATCGGGGAG CCTGAGAACA AGAGCAAGCA AAACGAAATG 
CTGGTGGCAG CGGCGGCAGT GGGCGTGGCC ACAGTCTTTG CAGCTCCCTT CAGCGGCGTC 
CTGTTCAGCA TCGAGGTCAT GTCTTCCCAC TTCTCTGTCC GGGATTACTG GAGGGGCTTC 
TTTGCGGCCA CCTGCGGGGC CTTCATATTC CGGCTCCTGG CAGTCTTCAA CAGCGAGCAG 
GAGACCATCA CCTCCCTCTA CAAGACCAGT TTCCGGGTGG ACGTTCCCTT CGACCTGCCT 
GAGATCTTCT TTTTTGTGGC GCTGGGTGGC ATCTGCGGCG TCCTGAGCTG TGCTTACCTC 
TTCTGTCAGC GAACCTTCCT CAGCTTCATC AAGACCAATC GGTACAGCTC CAAACTGCTG 
GCTACTAGCA AGCCTGTGTA CTCCGCTCTG GCCACCTTGC TTCTCGCCTC CATCACCTAC 
CCGCCTGGTG TGGGCCACTT CCTAGCTTCT CGGCTGTCCA TGAAGCAGCA TCTGGACTCG 
CTGTTCGACA ACCACTCCTG GGCGCTGATG ACCCAGAACT CCAGCCCACC CTGGCCCGAG 
GAGCTCGACC CCCAGCACCT TTGGTGGGAA TGGTACCACC CGCGGTTCAC CATCTTTGGG 
ACCCTTGCCT TCTTCCTGGT TATGAAGTTC TGGATGCTGA TTCTGGCCAC CACCATCCCC 
ATGCCTGCCG GGTACTTCAT GCCCATCTTT ATCCTTGGAG CTGCCATCGG GCGCCTCTTG 
GGAGAGGCTC TTGCCGTCGC CTTCCCTGAG GGCATTGTGA CTGGAGGGGT TACCAATCCC 
ATCATGCCCG GGGGGTATGC TCTGGCAGGG GCTGCAGCCT TCTCAGGGGC TGTGACCCAC 
ACCATCTCCA CGGCGCTGCT GGCCTTTGAG CTGACCGGCC AGATAGTGCA TGCACTGCCC 
GTGCTGATGG CGGTGCTGGC AGCCAACGCC ATTGCACAGA GCTGCCAGCC CTCCTTCTAT 
GATGGCACCA TCATTGTCAA GAAGCTGCCA TACCTGCCAC GGATTCTGGG CCGCAACATC 
GGCTCCCACC ATGTGAGGGT GGAGCACTTC ATGAACCACA GCATCACCAC ACTGGCCAAG 
GACACGCCGC TGGAGGAGGT GGTCAAGGTT GTGACCTCCA CAGACGTGAC CGAGTATCCC 
CTGGTGGAGA GCACAGAGTC CCAGATCCTG GTAGGCATCG TGCAGAGGGC CCAGCTGGTG 
CAGGCCCTCC AGGCTGAGCC TCCTTCCAGG GCTCCAGGAC ACCAGTGTCT CCAGGACATC 
TTGGCCAGGG GCTGCCCCAC GGAACCAGTG ACCCTGACGC TATTCTCAGA GACCACCTTG 
CACCAGGCAC AAAACCTCTT TAAGCTGTTG AACCTTCAGT CCCTCTTCGT GACATCGCGG 
GGCAGAGCTG TGGGCTGCGT GTCCTGGGTG GAGATGAAGA AAGCAATTTC CAACCTGACA 
AATCCGCCAG CTCCAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCNKA ( NM_001042704.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001036169.1 Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 2, mRNA.

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CloneID	OHu30105
Clone ID Related Accession (Same CDS sequence)	NM_001042704.1 , NM_001042704.2
Accession Version	NM_001042704.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	chloride channel protein ClC-Ka isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK225550.1 and BC053869.1. On May 31, 2019 this sequence version replaced NM_001042704.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a protein (isoform 2) that is one amino acid shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225550.1, BC053869.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGT TGGTGGGGCT GCGTGAGGGC TTCTCAGGGG ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCA CATCCGCCGA GCCATCCAAG GTGGCCTGGA GTGGCTAAAG 
CAGAAGGTGT TCCGCCTGGG AGAAGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTATGCCAT GAACTTTGCC ATCGGGTGTG TGGTCCGAGC ACACCAGTGG 
CTGTACAGGG AGATTGGGGA CAGCCACCTG CTCCGGTATC TTTCCTGGAC TGTGTACCCT 
GTGGCCCTCG TCTCTTTCTC CTCAGGCTTC TCCCAGAGCA TCACGCCCTC CTCTGGAGGT 
TCTGGAATCC CGGAGCTGAA GACCATGTTG GCGGGTGTGA TCTTGGAGGA CTACCTGGAT 
ATCAAGAACT TTGGGGCCAA GGTGGTGGGC CTCTCCTGCA CCCTGGCCAC CGGCAGCACC 
CTGTTCCTGG GCAAAGTGGG CCCTTTCGTG CACCTGTCTG TAATGATCGC TGCCTACCTG 
GGCCGTGTGC GCACCACGAC CATCGGGGAG CCTGAGAACA AGAGCAAGCA AAACGAAATG 
CTGGTGGCAG CGGCGGCAGT GGGCGTGGCC ACAGTCTTTG CAGCTCCCTT CAGCGGCGTC 
CTGTTCAGCA TCGAGGTCAT GTCTTCCCAC TTCTCTGTCC GGGATTACTG GAGGGGCTTC 
TTTGCGGCCA CCTGCGGGGC CTTCATATTC CGGCTCCTGG CAGTCTTCAA CAGCGAGCAG 
GAGACCATCA CCTCCCTCTA CAAGACCAGT TTCCGGGTGG ACGTTCCCTT CGACCTGCCT 
GAGATCTTCT TTTTTGTGGC GCTGGGTGGC ATCTGCGGCG TCCTGAGCTG TGCTTACCTC 
TTCTGTCAGC GAACCTTCCT CAGCTTCATC AAGACCAATC GGTACAGCTC CAAACTGCTG 
GCTACTAGCA AGCCTGTGTA CTCCGCTCTG GCCACCTTGC TTCTCGCCTC CATCACCTAC 
CCGCCTGGTG TGGGCCACTT CCTAGCTTCT CGGCTGTCCA TGAAGCAGCA TCTGGACTCG 
CTGTTCGACA ACCACTCCTG GGCGCTGATG ACCCAGAACT CCAGCCCACC CTGGCCCGAG 
GAGCTCGACC CCCAGCACCT TTGGTGGGAA TGGTACCACC CGCGGTTCAC CATCTTTGGG 
ACCCTTGCCT TCTTCCTGGT TATGAAGTTC TGGATGCTGA TTCTGGCCAC CACCATCCCC 
ATGCCTGCCG GGTACTTCAT GCCCATCTTT ATCCTTGGAG CTGCCATCGG GCGCCTCTTG 
GGAGAGGCTC TTGCCGTCGC CTTCCCTGAG GGCATTGTGA CTGGAGGGGT TACCAATCCC 
ATCATGCCCG GGGGGTATGC TCTGGCAGGG GCTGCAGCCT TCTCAGGGGC TGTGACCCAC 
ACCATCTCCA CGGCGCTGCT GGCCTTTGAG CTGACCGGCC AGATAGTGCA TGCACTGCCC 
GTGCTGATGG CGGTGCTGGC AGCCAACGCC ATTGCACAGA GCTGCCAGCC CTCCTTCTAT 
GATGGCACCA TCATTGTCAA GAAGCTGCCA TACCTGCCAC GGATTCTGGG CCGCAACATC 
GGCTCCCACC ATGTGAGGGT GGAGCACTTC ATGAACCACA GCATCACCAC ACTGGCCAAG 
GACACGCCGC TGGAGGAGGT GGTCAAGGTT GTGACCTCCA CAGACGTGAC CGAGTATCCC 
CTGGTGGAGA GCACAGAGTC CCAGATCCTG GTAGGCATCG TGCAGAGGGC CCAGCTGGTG 
CAGGCCCTCC AGGCTGAGCC TCCTTCCAGG GCTCCAGGAC ACCAGTGTCT CCAGGACATC 
TTGGCCAGGG GCTGCCCCAC GGAACCAGTG ACCCTGACGC TATTCTCAGA GACCACCTTG 
CACCAGGCAC AAAACCTCTT TAAGCTGTTG AACCTTCAGT CCCTCTTCGT GACATCGCGG 
GGCAGAGCTG TGGGCTGCGT GTCCTGGGTG GAGATGAAGA AAGCAATTTC CAACCTGACA 
AATCCGCCAG CTCCAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036169.1
CDS	36..2096
Translation	MEELVGLREGFSGDPVTLQELWGPCPHIRRAIQGGLEWLKQKVFRLGEDWYFLMTLGVLMALVSYAMNFAIGCVVRAHQWLYREIGDSHLLRYLSWTVYPVALVSFSSGFSQSITPSSGGSGIPELKTMLAGVILEDYLDIKNFGAKVVGLSCTLATGSTLFLGKVGPFVHLSVMIAAYLGRVRTTTIGEPENKSKQNEMLVAAAAVGVATVFAAPFSGVLFSIEVMSSHFSVRDYWRGFFAATCGAFIFRLLAVFNSEQETITSLYKTSFRVDVPFDLPEIFFFVALGGICGVLSCAYLFCQRTFLSFIKTNRYSSKLLATSKPVYSALATLLLASITYPPGVGHFLASRLSMKQHLDSLFDNHSWALMTQNSSPPWPEELDPQHLWWEWYHPRFTIFGTLAFFLVMKFWMLILATTIPMPAGYFMPIFILGAAIGRLLGEALAVAFPEGIVTGGVTNPIMPGGYALAGAAAFSGAVTHTISTALLAFELTGQIVHALPVLMAVLAANAIAQSCQPSFYDGTIIVKKLPYLPRILGRNIGSHHVRVEHFMNHSITTLAKDTPLEEVVKVVTSTDVTEYPLVESTESQILVGIVQRAQLVQALQAEPPSRAPGHQCLQDILARGCPTEPVTLTLFSETTLHQAQNLFKLLNLQSLFVTSRGRAVGCVSWVEMKKAISNLTNPPAPK

Target ORF information:

RefSeq Version	NM_001042704.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel Ka (CLCNKA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042704.2

ORF Insert Sequence:

ATGGAGGAGT TGGTGGGGCT GCGTGAGGGC TTCTCAGGGG ACCCTGTGAC TCTGCAGGAG 
CTGTGGGGCC CCTGTCCCCA CATCCGCCGA GCCATCCAAG GTGGCCTGGA GTGGCTAAAG 
CAGAAGGTGT TCCGCCTGGG AGAAGACTGG TACTTCCTGA TGACCCTCGG GGTGCTCATG 
GCCCTGGTCA GCTATGCCAT GAACTTTGCC ATCGGGTGTG TGGTCCGAGC ACACCAGTGG 
CTGTACAGGG AGATTGGGGA CAGCCACCTG CTCCGGTATC TTTCCTGGAC TGTGTACCCT 
GTGGCCCTCG TCTCTTTCTC CTCAGGCTTC TCCCAGAGCA TCACGCCCTC CTCTGGAGGT 
TCTGGAATCC CGGAGCTGAA GACCATGTTG GCGGGTGTGA TCTTGGAGGA CTACCTGGAT 
ATCAAGAACT TTGGGGCCAA GGTGGTGGGC CTCTCCTGCA CCCTGGCCAC CGGCAGCACC 
CTGTTCCTGG GCAAAGTGGG CCCTTTCGTG CACCTGTCTG TAATGATCGC TGCCTACCTG 
GGCCGTGTGC GCACCACGAC CATCGGGGAG CCTGAGAACA AGAGCAAGCA AAACGAAATG 
CTGGTGGCAG CGGCGGCAGT GGGCGTGGCC ACAGTCTTTG CAGCTCCCTT CAGCGGCGTC 
CTGTTCAGCA TCGAGGTCAT GTCTTCCCAC TTCTCTGTCC GGGATTACTG GAGGGGCTTC 
TTTGCGGCCA CCTGCGGGGC CTTCATATTC CGGCTCCTGG CAGTCTTCAA CAGCGAGCAG 
GAGACCATCA CCTCCCTCTA CAAGACCAGT TTCCGGGTGG ACGTTCCCTT CGACCTGCCT 
GAGATCTTCT TTTTTGTGGC GCTGGGTGGC ATCTGCGGCG TCCTGAGCTG TGCTTACCTC 
TTCTGTCAGC GAACCTTCCT CAGCTTCATC AAGACCAATC GGTACAGCTC CAAACTGCTG 
GCTACTAGCA AGCCTGTGTA CTCCGCTCTG GCCACCTTGC TTCTCGCCTC CATCACCTAC 
CCGCCTGGTG TGGGCCACTT CCTAGCTTCT CGGCTGTCCA TGAAGCAGCA TCTGGACTCG 
CTGTTCGACA ACCACTCCTG GGCGCTGATG ACCCAGAACT CCAGCCCACC CTGGCCCGAG 
GAGCTCGACC CCCAGCACCT TTGGTGGGAA TGGTACCACC CGCGGTTCAC CATCTTTGGG 
ACCCTTGCCT TCTTCCTGGT TATGAAGTTC TGGATGCTGA TTCTGGCCAC CACCATCCCC 
ATGCCTGCCG GGTACTTCAT GCCCATCTTT ATCCTTGGAG CTGCCATCGG GCGCCTCTTG 
GGAGAGGCTC TTGCCGTCGC CTTCCCTGAG GGCATTGTGA CTGGAGGGGT TACCAATCCC 
ATCATGCCCG GGGGGTATGC TCTGGCAGGG GCTGCAGCCT TCTCAGGGGC TGTGACCCAC 
ACCATCTCCA CGGCGCTGCT GGCCTTTGAG CTGACCGGCC AGATAGTGCA TGCACTGCCC 
GTGCTGATGG CGGTGCTGGC AGCCAACGCC ATTGCACAGA GCTGCCAGCC CTCCTTCTAT 
GATGGCACCA TCATTGTCAA GAAGCTGCCA TACCTGCCAC GGATTCTGGG CCGCAACATC 
GGCTCCCACC ATGTGAGGGT GGAGCACTTC ATGAACCACA GCATCACCAC ACTGGCCAAG 
GACACGCCGC TGGAGGAGGT GGTCAAGGTT GTGACCTCCA CAGACGTGAC CGAGTATCCC 
CTGGTGGAGA GCACAGAGTC CCAGATCCTG GTAGGCATCG TGCAGAGGGC CCAGCTGGTG 
CAGGCCCTCC AGGCTGAGCC TCCTTCCAGG GCTCCAGGAC ACCAGTGTCT CCAGGACATC 
TTGGCCAGGG GCTGCCCCAC GGAACCAGTG ACCCTGACGC TATTCTCAGA GACCACCTTG 
CACCAGGCAC AAAACCTCTT TAAGCTGTTG AACCTTCAGT CCCTCTTCGT GACATCGCGG 
GGCAGAGCTG TGGGCTGCGT GTCCTGGGTG GAGATGAAGA AAGCAATTTC CAACCTGACA 
AATCCGCCAG CTCCAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human CLC-K Channels Require Palmitoylation of Their Accessory Subunit Barttin to Be Functional.
The Journal of biological chemistry290(28)17390-400(2015 Jul)
Steinke KV,Gorinski N,Wojciechowski D,Todorov V,Guseva D,Ponimaskin E,Fahlke C,Fischer M

Associations between CLCNKA_B tag SNPs with essential hypertension and interactions between genetic and environmental factors in an island population in China.
Clinical and experimental hypertension (New York, N.Y. : 1993)37(7)519-25(2015)
Chen X,Zhou B,Hou X,Xing J,Zou S,Wu X,Ma B,Shi J

Targeting kidney CLC-K channels: pharmacological profile in a human cell line versus Xenopus oocytes.
Biochimica et biophysica acta1838(10)2484-91(2014 Oct)
Imbrici P,Liantonio A,Gradogna A,Pusch M,Camerino DC

A CLCNKA polymorphism (rs10927887; p.Arg83Gly) previously linked to heart failure is associated with the estimated glomerular filtration rate in the RENASTUR cohort.
Gene527(2)670-2(2013 Sep)
Tavira B,G?mez J,Ortega F,Tranche S,D?az-Corte C,Alvarez F,Ortiz A,Santos F,S?nchez-Ni?o MD,Coto E

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CLCNKA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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