CLCN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLCN1
Entrez Gene ID	1180
Full Name	chloride voltage-gated channel 1
Synonyms	CLC1
General protein information	Preferred Names chloride voltage-gated channel 1 Names chloride channel protein 1 chloride channel 1, skeletal muscle chloride channel protein, skeletal muscle chloride channel, voltage-sensitive 1 clC-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q34
Summary	The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. The protein encoded by this gene regulates the electric excitability of the skeletal muscle membrane. Mutations in this gene cause two forms of inherited human muscle disorders: recessive generalized myotonia congenita (Becker) and dominant myotonia (Thomsen). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012].
Disorder MIM:	118425
Disorder Html:	Myotonia congenita, recessive, 255700 (3); Myotonia congenita, dominant, 160800 (3); Myotonia levior, recessive (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000083.3	NP_000074.3	chloride channel protein 1
NM_000083.2	NP_000074.2	chloride channel protein 1
XM_017011739.1	XP_016867228.1	chloride channel protein 1 isoform X1
XM_017011740.1	XP_016867229.1	chloride channel protein 1 isoform X2
XM_011515782.2	XP_011514084.1	chloride channel protein 1 isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Clcn1	NP_038519.1
Gallus gallus (chicken)	CLCN1	XP_004938180.1
Danio rerio (zebrafish)	LOC567478	XP_695866.4
	CLCN1	XP_001092282.1
Danio rerio (zebrafish)	clcn1a	NP_001076379.1
Homo sapiens (human)	CLCN1	NP_000074.2
Pan troglodytes (chimpanzee)	CLCN1	XP_527935.2
Canis lupus familiaris (dog)	CLCN1	NP_001003124.1
Bos taurus (cattle)	CLCN1	NP_001137343.1
Rattus norvegicus (Norway rat)	Clcn1	NP_037279.1

Gene Ontology

The following CLCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26773	NM_000083.2 Latest version!	Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu56922	XM_011515782.2 Latest version!	Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu108602	NM_000083.3 Latest version!	Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu97036	XM_017011739.1 Latest version!	Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu97037	XM_017011740.1 Latest version!	Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLCN1 ( NM_000083.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000074.2 Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26773
Clone ID Related Accession (Same CDS sequence)	NM_000083.2
Accession Version	NM_000083.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	chloride channel protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z25884.1, BC112156.1 and AC073342.3. This sequence is a reference standard in the RefSeqGene project. On Dec 15, 2006 this sequence version replaced NM_000083.1. Transcript Variant: This variant (1) encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z25884.1, BC112156.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158800, SAMN03465413 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAT CCCGGTCACA GCAGCGTGGG GGTGAACAAA GCTGGTGGGG TAGTGACCCC 
CAGTACCAGT ATATGCCCTT TGAACACTGC ACCAGCTACG GACTGCCCTC TGAGAATGGG 
GGCCTCCAGC ACAGGCTCCG GAAGGATGCA GGCCCCCGCC ACAACGTCCA CCCCACACAG 
ATTTATGGCC ATCACAAAGA ACAATTCTCA GACAGGGAGC AGGACATAGG GATGCCCAAG 
AAGACAGGCT CCAGTTCTAC CGTGGACAGC AAGGATGAGG ATCACTATTC TAAATGTCAA 
GATTGTATCC ACCGCCTGGG ACAGGTGGTG AGAAGAAAAT TAGGGGAAGA CTGGATCTTT 
CTGGTGCTTC TGGGACTGCT GATGGCTCTG GTCAGCTGGA GCATGGACTA CGTCAGTGCC 
AAAAGCCTTC AGGCCTACAA GTGGTCCTAC GCGCAGATGC AGCCCAGCCT TCCTCTGCAG 
TTCCTGGTCT GGGTCACCTT CCCACTAGTC CTCATCCTCT TCAGCGCCCT CTTCTGCCAC 
CTCATCTCTC CCCAGGCTGT TGGCTCTGGA ATCCCCGAAA TGAAGACAAT ACTTCGTGGG 
GTTGTCCTGA AGGAATACCT CACAATGAAA GCCTTTGTGG CCAAGGTTGT CGCCCTGACT 
GCGGGCCTGG GCAGTGGCAT CCCCGTGGGG AAAGAGGGCC CCTTCGTCCA CATTGCCAGC 
ATCTGTGCTG CTGTCCTCAG CAAATTCATG TCTGTGTTCT GCGGGGTATA TGAGCAGCCA 
TACTACTACT CTGATATCCT GACGGTGGGC TGTGCTGTGG GAGTCGGCTG TTGTTTTGGG 
ACACCACTTG GAGGAGTGCT ATTTAGCATC GAGGTCACCT CCACCTACTT TGCTGTTCGG 
AACTACTGGA GAGGATTCTT TGCAGCCACG TTCAGCGCCT TTGTGTTTCG AGTGCTGGCA 
GTGTGGAACA AGGATGCTGT CACCATCACT GCTCTGTTCA GAACCAATTT CCGAATGGAT 
TTCCCCTTTG ACCTGAAGGA ACTACCAGCT TTTGCTGCCA TCGGGATTTG CTGTGGGCTC 
CTGGGAGCTG TATTTGTGTA TCTGCATCGC CAAGTCATGC TCGGTGTCCG AAAGCACAAG 
GCCCTCAGCC AGTTTCTTGC TAAGCACCGC CTGCTGTATC CTGGAATTGT TACCTTTGTC 
ATTGCCTCAT TCACCTTCCC ACCAGGAATG GGTCAATTCA TGGCTGGAGA GTTGATGCCC 
CGCGAAGCCA TCAGTACTTT GTTTGACAAC AATACATGGG TGAAACACGC GGGTGATCCT 
GAGAGCCTGG GCCAGTCAGC TGTGTGGATT CACCCCCGGG TCAACGTTGT CATCATCATC 
TTTCTCTTCT TCGTCATGAA GTTCTGGATG TCCATCGTGG CCACCACTAT GCCCATACCC 
TGCGGAGGCT TCATGCCTGT GTTTGTGCTA GGAGCTGCAT TTGGAAGGCT GGTAGGAGAA 
ATCATGGCCA TGCTCTTTCC TGATGGTATT TTGTTTGATG ACATCATCTA CAAGATCCTA 
CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA GCGCTGACTG GTGCCGTTTC CCACACAGTC 
TCCACAGCTG TGATTTGCTT CGAATTAACG GGTCAGATTG CTCACATCCT GCCCATGATG 
GTGGCTGTTA TCTTGGCCAA CATGGTGGCC CAGAGCCTGC AGCCCTCTCT CTATGACAGC 
ATCATCCAGG TCAAGAAGCT ACCCTACTTG CCTGACCTTG GCTGGAACCA GCTCAGCAAA 
TATACCATCT TTGTTGAGGA CATCATGGTA CGTGATGTGA AGTTTGTTTC AGCTTCTTAC 
ACATATGGGG AGTTGCGAAC CCTGCTCCAG ACCACCACAG TCAAGACTTT ACCACTGGTT 
GACTCAAAAG ATTCAATGAT CCTGCTGGGC TCGGTGGAGC GGTCGGAACT GCAGGCCCTC 
CTGCAGCGCC ACCTGTGTCC TGAGCGCAGG CTGCGCGCAG CCCAAGAGAT GGCGCGGAAG 
TTGTCGGAGC TGCCTTACGA CGGGAAGGCG CGGCTGGCTG GGGAGGGGCT CCCCGGCGCG 
CCTCCAGGCC GGCCCGAGTC CTTCGCCTTT GTGGATGAGG ATGAGGACGA AGACCTCTCT 
GGCAAGAGCG AGCTTCCTCC TTCCCTTGCT CTCCACCCCT CTACTACTGC CCCTCTGTCC 
CCAGAAGAGC CCAATGGGCC TCTGCCTGGC CACAAACAGC AGCCGGAAGC ACCAGAGCCT 
GCAGGTCAAA GACCCTCCAT CTTCCAGTCC CTGCTTCACT GCTTGCTGGG CAGAGCTCGC 
CCCACAAAGA AGAAAACAAC CCAGGATTCC ACAGATTTAG TGGATAACAT GTCACCTGAA 
GAGATTGAGG CCTGGGAGCA GGAGCAGCTG AGCCAGCCTG TCTGTTTTGA TTCCTGCTGT 
ATTGACCAGT CTCCCTTCCA GCTGGTGGAG CAGACAACCC TGCACAAGAC TCATACCCTG 
TTTTCACTCC TTGGCCTCCA CCTCGCTTAC GTGACCAGCA TGGGGAAGCT CAGGGGCGTC 
CTGGCCCTGG AGGAGCTACA GAAGGCCATT GAGGGGCACA CCAAGTCTGG GGTGCAGCTC 
CGCCCTCCCC TTGCCAGCTT CCGGAACACG ACTTCAACTC GAAAGAGTAC CGGGGCACCT 
CCATCTTCTG CAGAGAACTG GAACCTGCCT GAGGACAGGC CTGGGGCCAC TGGAACAGGG 
GATGTGATTG CTGCCTCCCC AGAGACCCCT GTGCCATCTC CTTCCCCAGA GCCCCCTCTC 
TCCCTGGCCC CAGGCAAGGT AGAGGGCGAG TTGGAGGAGC TGGAGCTGGT GGAGAGTCCA 
GGGCTGGAAG AGGAGCTGGC CGACATCTTG CAGGGCCCCA GCCTGCGATC CACAGACGAG 
GAGGATGAGG ATGAACTGAT CCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000074.2
CDS	88..3054
Translation	MEQSRSQQRGGEQSWWGSDPQYQYMPFEHCTSYGLPSENGGLQHRLRKDAGPRHNVHPTQIYGHHKEQFSDREQDIGMPKKTGSSSTVDSKDEDHYSKCQDCIHRLGQVVRRKLGEDWIFLVLLGLLMALVSWSMDYVSAKSLQAYKWSYAQMQPSLPLQFLVWVTFPLVLILFSALFCHLISPQAVGSGIPEMKTILRGVVLKEYLTMKAFVAKVVALTAGLGSGIPVGKEGPFVHIASICAAVLSKFMSVFCGVYEQPYYYSDILTVGCAVGVGCCFGTPLGGVLFSIEVTSTYFAVRNYWRGFFAATFSAFVFRVLAVWNKDAVTITALFRTNFRMDFPFDLKELPAFAAIGICCGLLGAVFVYLHRQVMLGVRKHKALSQFLAKHRLLYPGIVTFVIASFTFPPGMGQFMAGELMPREAISTLFDNNTWVKHAGDPESLGQSAVWIHPRVNVVIIIFLFFVMKFWMSIVATTMPIPCGGFMPVFVLGAAFGRLVGEIMAMLFPDGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMMVAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKYTIFVEDIMVRDVKFVSASYTYGELRTLLQTTTVKTLPLVDSKDSMILLGSVERSELQALLQRHLCPERRLRAAQEMARKLSELPYDGKARLAGEGLPGAPPGRPESFAFVDEDEDEDLSGKSELPPSLALHPSTTAPLSPEEPNGPLPGHKQQPEAPEPAGQRPSIFQSLLHCLLGRARPTKKKTTQDSTDLVDNMSPEEIEAWEQEQLSQPVCFDSCCIDQSPFQLVEQTTLHKTHTLFSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSTRKSTGAPPSSAENWNLPEDRPGATGTGDVIAASPETPVPSPSPEPPLSLAPGKVEGELEELELVESPGLEEELADILQGPSLRSTDEEDEDELIL

Target ORF information:

RefSeq Version	NM_000083.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000083.2

ORF Insert Sequence:

ATGGAGCAAT CCCGGTCACA GCAGCGTGGG GGTGAACAAA GCTGGTGGGG TAGTGACCCC 
CAGTACCAGT ATATGCCCTT TGAACACTGC ACCAGCTACG GACTGCCCTC TGAGAATGGG 
GGCCTCCAGC ACAGGCTCCG GAAGGATGCA GGCCCCCGCC ACAACGTCCA CCCCACACAG 
ATTTATGGCC ATCACAAAGA ACAATTCTCA GACAGGGAGC AGGACATAGG GATGCCCAAG 
AAGACAGGCT CCAGTTCTAC CGTGGACAGC AAGGATGAGG ATCACTATTC TAAATGTCAA 
GATTGTATCC ACCGCCTGGG ACAGGTGGTG AGAAGAAAAT TAGGGGAAGA CTGGATCTTT 
CTGGTGCTTC TGGGACTGCT GATGGCTCTG GTCAGCTGGA GCATGGACTA CGTCAGTGCC 
AAAAGCCTTC AGGCCTACAA GTGGTCCTAC GCGCAGATGC AGCCCAGCCT TCCTCTGCAG 
TTCCTGGTCT GGGTCACCTT CCCACTAGTC CTCATCCTCT TCAGCGCCCT CTTCTGCCAC 
CTCATCTCTC CCCAGGCTGT TGGCTCTGGA ATCCCCGAAA TGAAGACAAT ACTTCGTGGG 
GTTGTCCTGA AGGAATACCT CACAATGAAA GCCTTTGTGG CCAAGGTTGT CGCCCTGACT 
GCGGGCCTGG GCAGTGGCAT CCCCGTGGGG AAAGAGGGCC CCTTCGTCCA CATTGCCAGC 
ATCTGTGCTG CTGTCCTCAG CAAATTCATG TCTGTGTTCT GCGGGGTATA TGAGCAGCCA 
TACTACTACT CTGATATCCT GACGGTGGGC TGTGCTGTGG GAGTCGGCTG TTGTTTTGGG 
ACACCACTTG GAGGAGTGCT ATTTAGCATC GAGGTCACCT CCACCTACTT TGCTGTTCGG 
AACTACTGGA GAGGATTCTT TGCAGCCACG TTCAGCGCCT TTGTGTTTCG AGTGCTGGCA 
GTGTGGAACA AGGATGCTGT CACCATCACT GCTCTGTTCA GAACCAATTT CCGAATGGAT 
TTCCCCTTTG ACCTGAAGGA ACTACCAGCT TTTGCTGCCA TCGGGATTTG CTGTGGGCTC 
CTGGGAGCTG TATTTGTGTA TCTGCATCGC CAAGTCATGC TCGGTGTCCG AAAGCACAAG 
GCCCTCAGCC AGTTTCTTGC TAAGCACCGC CTGCTGTATC CTGGAATTGT TACCTTTGTC 
ATTGCCTCAT TCACCTTCCC ACCAGGAATG GGTCAATTCA TGGCTGGAGA GTTGATGCCC 
CGCGAAGCCA TCAGTACTTT GTTTGACAAC AATACATGGG TGAAACACGC GGGTGATCCT 
GAGAGCCTGG GCCAGTCAGC TGTGTGGATT CACCCCCGGG TCAACGTTGT CATCATCATC 
TTTCTCTTCT TCGTCATGAA GTTCTGGATG TCCATCGTGG CCACCACTAT GCCCATACCC 
TGCGGAGGCT TCATGCCTGT GTTTGTGCTA GGAGCTGCAT TTGGAAGGCT GGTAGGAGAA 
ATCATGGCCA TGCTCTTTCC TGATGGTATT TTGTTTGATG ACATCATCTA CAAGATCCTA 
CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA GCGCTGACTG GTGCCGTTTC CCACACAGTC 
TCCACAGCTG TGATTTGCTT CGAATTAACG GGTCAGATTG CTCACATCCT GCCCATGATG 
GTGGCTGTTA TCTTGGCCAA CATGGTGGCC CAGAGCCTGC AGCCCTCTCT CTATGACAGC 
ATCATCCAGG TCAAGAAGCT ACCCTACTTG CCTGACCTTG GCTGGAACCA GCTCAGCAAA 
TATACCATCT TTGTTGAGGA CATCATGGTA CGTGATGTGA AGTTTGTTTC AGCTTCTTAC 
ACATATGGGG AGTTGCGAAC CCTGCTCCAG ACCACCACAG TCAAGACTTT ACCACTGGTT 
GACTCAAAAG ATTCAATGAT CCTGCTGGGC TCGGTGGAGC GGTCGGAACT GCAGGCCCTC 
CTGCAGCGCC ACCTGTGTCC TGAGCGCAGG CTGCGCGCAG CCCAAGAGAT GGCGCGGAAG 
TTGTCGGAGC TGCCTTACGA CGGGAAGGCG CGGCTGGCTG GGGAGGGGCT CCCCGGCGCG 
CCTCCAGGCC GGCCCGAGTC CTTCGCCTTT GTGGATGAGG ATGAGGACGA AGACCTCTCT 
GGCAAGAGCG AGCTTCCTCC TTCCCTTGCT CTCCACCCCT CTACTACTGC CCCTCTGTCC 
CCAGAAGAGC CCAATGGGCC TCTGCCTGGC CACAAACAGC AGCCGGAAGC ACCAGAGCCT 
GCAGGTCAAA GACCCTCCAT CTTCCAGTCC CTGCTTCACT GCTTGCTGGG CAGAGCTCGC 
CCCACAAAGA AGAAAACAAC CCAGGATTCC ACAGATTTAG TGGATAACAT GTCACCTGAA 
GAGATTGAGG CCTGGGAGCA GGAGCAGCTG AGCCAGCCTG TCTGTTTTGA TTCCTGCTGT 
ATTGACCAGT CTCCCTTCCA GCTGGTGGAG CAGACAACCC TGCACAAGAC TCATACCCTG 
TTTTCACTCC TTGGCCTCCA CCTCGCTTAC GTGACCAGCA TGGGGAAGCT CAGGGGCGTC 
CTGGCCCTGG AGGAGCTACA GAAGGCCATT GAGGGGCACA CCAAGTCTGG GGTGCAGCTC 
CGCCCTCCCC TTGCCAGCTT CCGGAACACG ACTTCAACTC GAAAGAGTAC CGGGGCACCT 
CCATCTTCTG CAGAGAACTG GAACCTGCCT GAGGACAGGC CTGGGGCCAC TGGAACAGGG 
GATGTGATTG CTGCCTCCCC AGAGACCCCT GTGCCATCTC CTTCCCCAGA GCCCCCTCTC 
TCCCTGGCCC CAGGCAAGGT AGAGGGCGAG TTGGAGGAGC TGGAGCTGGT GGAGAGTCCA 
GGGCTGGAAG AGGAGCTGGC CGACATCTTG CAGGGCCCCA GCCTGCGATC CACAGACGAG 
GAGGATGAGG ATGAACTGAT CCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCN1 ( XM_011515782.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514084.1 Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu56922
Clone ID Related Accession (Same CDS sequence)	XM_011515782.2
Accession Version	XM_011515782.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	chloride channel protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCGCG AAGCCATCAG TACTTTGTTT GACAACAATA CATGGGTGAA ACACGCGGGT 
GATCCTGAGA GCCTGGGCCA GTCAGCTGTG TGGATTCACC CCCGGGTCAA CGTTGTCATC 
ATCATCTTTC TCTTCTTCGT CATGAAGTTC TGGATGTCCA TCGTGGCCAC CACTATGCCC 
ATACCCTGCG GAGGCTTCAT GCCTGTGTTT GTGCTAGGAG CTGCATTTGG AAGGCTGGTA 
GGAGAAATCA TGGCCATGCT CTTTCCTGAT GGTATTTTGT TTGATGACAT CATCTACAAG 
ATCCTACCTG GGGGCTATGC AGTAATTGGA GCAGCAGCGC TGACTGGTGC CGTTTCCCAC 
ACAGTCTCCA CAGCTGTGAT TTGCTTCGAA TTAACGGGTC AGATTGCTCA CATCCTGCCC 
ATGATGGTGG CTGTTATCTT GGCCAACATG GTGGCCCAGA GCCTGCAGCC CTCTCTCTAT 
GACAGCATCA TCCAGGTCAA GAAGCTACCC TACTTGCCTG ACCTTGGCTG GAACCAGCTC 
AGCAAATATA CCATCTTTGT TGAGGACATC ATGGTACGTG ATGTGAAGTT TGTTTCAGCT 
TCTTACACAT ATGGGGAGTT GCGAACCCTG CTCCAGACCA CCACAGTCAA GACTTTACCA 
CTGGTTGACT CAAAAGATTC AATGATCCTG CTGGGCTCGG TGGAGCGGTC GGAACTGCAG 
GCCCTCCTGC AGCGCCACCT GTGTCCTGAG CGCAGGCTGC GCGCAGCCCA AGAGATGGCG 
CGGAAGTTGT CGGAGCTGCC TTACGACGGG AAGGCGCGGC TGGCTGGGGA GGGGCTCCCC 
GGCGCGCCTC CAGGCCGGCC CGAGTCCTTC GCCTTTGTGG ATGAGGATGA GGACGAAGAC 
CTCTCTGGCA AGAGCGAGCT TCCTCCTTCC CTTGCTCTCC ACCCCTCTAC TACTGCCCCT 
CTGTCCCCAG AAGAGCCCAA TGGGCCTCTG CCTGGCCACA AACAGCAGCC GGAAGCACCA 
GAGCCTGCAG GTCAAAGACC CTCCATCTTC CAGTCCCTGC TTCACTGCTT GCTGGGCAGA 
GCTCGCCCCA CAAAGAAGAA AACAACCCAG GATTCCACAG ATTTAGTGGA TAACATGTCA 
CCTGAAGAGA TTGAGGCCTG GGAGCAGGAG CAGCTGAGCC AGCCTGTCTG TTTTGATTCC 
TGCTGTATTG ACCAGTCTCC CTTCCAGCTG GTGGAGCAGA CAACCCTGCA CAAGACTCAT 
ACCCTGTTTT CACTCCTTGG CCTCCACCTC GCTTACGTGA CCAGCATGGG GAAGCTCAGG 
GGCGTCCTGG CCCTGGAGGA GCTACAGAAG GCCATTGAGG GGCACACCAA GTCTGGGGTG 
CAGCTCCGCC CTCCCCTTGC CAGCTTCCGG AACACGACTT CAACTCGAAA GAGTACCGGG 
GCACCTCCAT CTTCTGCAGA GAACTGGAAC CTGCCTGAGG ACAGGCCTGG GGCCACTGGA 
ACAGGGGATG TGATTGCTGC CTCCCCAGAG ACCCCTGTGC CATCTCCTTC CCCAGAGCCC 
CCTCTCTCCC TGGCCCCAGG CAAGGTAGAG GGCGAGTTGG AGGAGCTGGA GCTGGTGGAG 
AGTCCAGGGC TGGAAGAGGA GCTGGCCGAC ATCTTGCAGG GCCCCAGCCT GCGATCCACA 
GACGAGGAGG ATGAGGATGA ACTGATCCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514084.1
CDS	90..1802
Translation	MPREAISTLFDNNTWVKHAGDPESLGQSAVWIHPRVNVVIIIFLFFVMKFWMSIVATTMPIPCGGFMPVFVLGAAFGRLVGEIMAMLFPDGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMMVAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKYTIFVEDIMVRDVKFVSASYTYGELRTLLQTTTVKTLPLVDSKDSMILLGSVERSELQALLQRHLCPERRLRAAQEMARKLSELPYDGKARLAGEGLPGAPPGRPESFAFVDEDEDEDLSGKSELPPSLALHPSTTAPLSPEEPNGPLPGHKQQPEAPEPAGQRPSIFQSLLHCLLGRARPTKKKTTQDSTDLVDNMSPEEIEAWEQEQLSQPVCFDSCCIDQSPFQLVEQTTLHKTHTLFSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSTRKSTGAPPSSAENWNLPEDRPGATGTGDVIAASPETPVPSPSPEPPLSLAPGKVEGELEELELVESPGLEEELADILQGPSLRSTDEEDEDELIL

Target ORF information:

RefSeq Version	XM_011515782.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515782.2

ORF Insert Sequence:

ATGCCCCGCG AAGCCATCAG TACTTTGTTT GACAACAATA CATGGGTGAA ACACGCGGGT 
GATCCTGAGA GCCTGGGCCA GTCAGCTGTG TGGATTCACC CCCGGGTCAA CGTTGTCATC 
ATCATCTTTC TCTTCTTCGT CATGAAGTTC TGGATGTCCA TCGTGGCCAC CACTATGCCC 
ATACCCTGCG GAGGCTTCAT GCCTGTGTTT GTGCTAGGAG CTGCATTTGG AAGGCTGGTA 
GGAGAAATCA TGGCCATGCT CTTTCCTGAT GGTATTTTGT TTGATGACAT CATCTACAAG 
ATCCTACCTG GGGGCTATGC AGTAATTGGA GCAGCAGCGC TGACTGGTGC CGTTTCCCAC 
ACAGTCTCCA CAGCTGTGAT TTGCTTCGAA TTAACGGGTC AGATTGCTCA CATCCTGCCC 
ATGATGGTGG CTGTTATCTT GGCCAACATG GTGGCCCAGA GCCTGCAGCC CTCTCTCTAT 
GACAGCATCA TCCAGGTCAA GAAGCTACCC TACTTGCCTG ACCTTGGCTG GAACCAGCTC 
AGCAAATATA CCATCTTTGT TGAGGACATC ATGGTACGTG ATGTGAAGTT TGTTTCAGCT 
TCTTACACAT ATGGGGAGTT GCGAACCCTG CTCCAGACCA CCACAGTCAA GACTTTACCA 
CTGGTTGACT CAAAAGATTC AATGATCCTG CTGGGCTCGG TGGAGCGGTC GGAACTGCAG 
GCCCTCCTGC AGCGCCACCT GTGTCCTGAG CGCAGGCTGC GCGCAGCCCA AGAGATGGCG 
CGGAAGTTGT CGGAGCTGCC TTACGACGGG AAGGCGCGGC TGGCTGGGGA GGGGCTCCCC 
GGCGCGCCTC CAGGCCGGCC CGAGTCCTTC GCCTTTGTGG ATGAGGATGA GGACGAAGAC 
CTCTCTGGCA AGAGCGAGCT TCCTCCTTCC CTTGCTCTCC ACCCCTCTAC TACTGCCCCT 
CTGTCCCCAG AAGAGCCCAA TGGGCCTCTG CCTGGCCACA AACAGCAGCC GGAAGCACCA 
GAGCCTGCAG GTCAAAGACC CTCCATCTTC CAGTCCCTGC TTCACTGCTT GCTGGGCAGA 
GCTCGCCCCA CAAAGAAGAA AACAACCCAG GATTCCACAG ATTTAGTGGA TAACATGTCA 
CCTGAAGAGA TTGAGGCCTG GGAGCAGGAG CAGCTGAGCC AGCCTGTCTG TTTTGATTCC 
TGCTGTATTG ACCAGTCTCC CTTCCAGCTG GTGGAGCAGA CAACCCTGCA CAAGACTCAT 
ACCCTGTTTT CACTCCTTGG CCTCCACCTC GCTTACGTGA CCAGCATGGG GAAGCTCAGG 
GGCGTCCTGG CCCTGGAGGA GCTACAGAAG GCCATTGAGG GGCACACCAA GTCTGGGGTG 
CAGCTCCGCC CTCCCCTTGC CAGCTTCCGG AACACGACTT CAACTCGAAA GAGTACCGGG 
GCACCTCCAT CTTCTGCAGA GAACTGGAAC CTGCCTGAGG ACAGGCCTGG GGCCACTGGA 
ACAGGGGATG TGATTGCTGC CTCCCCAGAG ACCCCTGTGC CATCTCCTTC CCCAGAGCCC 
CCTCTCTCCC TGGCCCCAGG CAAGGTAGAG GGCGAGTTGG AGGAGCTGGA GCTGGTGGAG 
AGTCCAGGGC TGGAAGAGGA GCTGGCCGAC ATCTTGCAGG GCCCCAGCCT GCGATCCACA 
GACGAGGAGG ATGAGGATGA ACTGATCCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCN1 ( NM_000083.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000074.3 Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant 1, mRNA.

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CloneID	OHu108602
Clone ID Related Accession (Same CDS sequence)	NM_000083.3
Accession Version	NM_000083.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	chloride channel protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073342.3 and AC093673.4. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_000083.2. Transcript Variant: This variant (1) encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z25884.1, BC113495.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158800, SAMN03465413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000343257.7/ ENSP00000339867.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAT CCCGGTCACA GCAGCGTGGG GGTGAACAAA GCTGGTGGGG TAGTGACCCC 
CAGTACCAGT ATATGCCCTT TGAACACTGC ACCAGCTACG GACTGCCCTC TGAGAATGGG 
GGCCTCCAGC ACAGGCTCCG GAAGGATGCA GGCCCCCGCC ACAACGTCCA CCCCACACAG 
ATTTATGGCC ATCACAAAGA ACAATTCTCA GACAGGGAGC AGGACATAGG GATGCCCAAG 
AAGACAGGCT CCAGTTCTAC CGTGGACAGC AAGGATGAGG ATCACTATTC TAAATGTCAA 
GATTGTATCC ACCGCCTGGG ACAGGTGGTG AGAAGAAAAT TAGGGGAAGA CGGGATCTTT 
CTGGTGCTTC TGGGACTGCT GATGGCTCTG GTCAGCTGGA GCATGGACTA CGTCAGTGCC 
AAAAGCCTTC AGGCCTACAA GTGGTCCTAC GCGCAGATGC AGCCCAGCCT TCCTCTGCAG 
TTCCTGGTCT GGGTCACCTT CCCACTAGTC CTCATCCTCT TCAGCGCCCT CTTCTGCCAC 
CTCATCTCTC CCCAGGCTGT TGGCTCTGGA ATCCCCGAAA TGAAGACAAT ACTTCGTGGG 
GTTGTCCTGA AGGAATACCT CACAATGAAA GCCTTTGTGG CCAAGGTTGT CGCCCTGACT 
GCGGGCCTGG GCAGTGGCAT CCCCGTGGGG AAAGAGGGCC CCTTCGTCCA CATTGCCAGC 
ATCTGTGCTG CTGTCCTCAG CAAATTCATG TCTGTGTTCT GCGGGGTATA TGAGCAGCCA 
TACTACTACT CTGATATCCT GACGGTGGGC TGTGCTGTGG GAGTCGGCTG TTGTTTTGGG 
ACACCACTTG GAGGAGTGCT ATTTAGCATC GAGGTCACCT CCACCTACTT TGCTGTTCGG 
AACTACTGGA GAGGATTCTT TGCAGCCACG TTCAGCGCCT TTGTGTTTCG AGTGCTGGCA 
GTGTGGAACA AGGATGCTGT CACCATCACT GCTCTGTTCA GAACCAATTT CCGAATGGAT 
TTCCCCTTTG ACCTGAAGGA ACTACCAGCT TTTGCTGCCA TCGGGATTTG CTGTGGGCTC 
CTGGGAGCTG TATTTGTGTA TCTGCATCGC CAAGTCATGC TCGGTGTCCG AAAGCACAAG 
GCCCTCAGCC AGTTTCTTGC TAAGCACCGC CTGCTGTATC CTGGAATTGT TACCTTTGTC 
ATTGCCTCAT TCACCTTCCC ACCAGGAATG GGTCAATTCA TGGCTGGAGA GTTGATGCCC 
CGCGAAGCCA TCAGTACTTT GTTTGACAAC AATACATGGG TGAAACACGC GGGTGATCCT 
GAGAGCCTGG GCCAGTCAGC TGTGTGGATT CACCCCCGGG TCAACGTTGT CATCATCATC 
TTTCTCTTCT TCGTCATGAA GTTCTGGATG TCCATCGTGG CCACCACTAT GCCCATACCC 
TGCGGAGGCT TCATGCCTGT GTTTGTGCTA GGAGCTGCAT TTGGAAGGCT GGTAGGAGAA 
ATCATGGCCA TGCTCTTTCC TGATGGTATT TTGTTTGATG ACATCATCTA CAAGATCCTA 
CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA GCGCTGACTG GTGCCGTTTC CCACACAGTC 
TCCACAGCTG TGATTTGCTT CGAATTAACG GGTCAGATTG CTCACATCCT GCCCATGATG 
GTGGCTGTTA TCTTGGCCAA CATGGTGGCC CAGAGCCTGC AGCCCTCTCT CTATGACAGC 
ATCATCCAGG TCAAGAAGCT ACCCTACTTG CCTGACCTTG GCTGGAACCA GCTCAGCAAA 
TATACCATCT TTGTTGAGGA CATCATGGTA CGTGATGTGA AGTTTGTTTC AGCTTCTTAC 
ACATATGGGG AGTTGCGAAC CCTGCTCCAG ACCACCACAG TCAAGACTTT ACCACTGGTT 
GACTCAAAAG ATTCAATGAT CCTGCTGGGC TCGGTGGAGC GGTCGGAACT GCAGGCCCTC 
CTGCAGCGCC ACCTGTGTCC TGAGCGCAGG CTGCGCGCAG CCCAAGAGAT GGCGCGGAAG 
TTGTCGGAGC TGCCTTACGA CGGGAAGGCG CGGCTGGCTG GGGAGGGGCT CCCCGGCGCG 
CCTCCAGGCC GGCCCGAGTC CTTCGCCTTT GTGGATGAGG ATGAGGACGA AGACCTCTCT 
GGCAAGAGCG AGCTTCCTCC TTCCCTTGCT CTCCACCCCT CTACTACTGC CCCTCTGTCC 
CCAGAAGAGC CCAATGGGCC TCTGCCTGGC CACAAACAGC AGCCGGAAGC ACCAGAGCCT 
GCAGGTCAAA GACCCTCCAT CTTCCAGTCC CTGCTTCACT GCTTGCTGGG CAGAGCTCGC 
CCCACAAAGA AGAAAACAAC CCAGGATTCC ACAGATTTAG TGGATAACAT GTCACCTGAA 
GAGATTGAGG CCTGGGAGCA GGAGCAGCTG AGCCAGCCTG TCTGTTTTGA TTCCTGCTGT 
ATTGACCAGT CTCCCTTCCA GCTGGTGGAG CAGACAACCC TGCACAAGAC TCATACCCTG 
TTTTCACTCC TTGGCCTCCA CCTCGCTTAC GTGACCAGCA TGGGGAAGCT CAGGGGCGTC 
CTGGCCCTGG AGGAGCTACA GAAGGCCATT GAGGGGCACA CCAAGTCTGG GGTGCAGCTC 
CGCCCTCCCC TTGCCAGCTT CCGGAACACG ACTTCAACTC GAAAGAGTAC CGGGGCACCT 
CCATCTTCTG CAGAGAACTG GAACCTGCCT GAGGACAGGC CTGGGGCCAC TGGAACAGGG 
GATGTGATTG CTGCCTCCCC AGAGACCCCT GTGCCATCTC CTTCCCCAGA GCCCCCTCTC 
TCCCTGGCCC CAGGCAAGGT AGAGGGCGAG TTGGAGGAGC TGGAGCTGGT GGAGAGTCCA 
GGGCTGGAAG AGGAGCTGGC CGACATCTTG CAGGGCCCCA GCCTGCGATC CACAGACGAG 
GAGGATGAGG ATGAACTGAT CCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000074.3
CDS	103..3069
Translation	MEQSRSQQRGGEQSWWGSDPQYQYMPFEHCTSYGLPSENGGLQHRLRKDAGPRHNVHPTQIYGHHKEQFSDREQDIGMPKKTGSSSTVDSKDEDHYSKCQDCIHRLGQVVRRKLGEDGIFLVLLGLLMALVSWSMDYVSAKSLQAYKWSYAQMQPSLPLQFLVWVTFPLVLILFSALFCHLISPQAVGSGIPEMKTILRGVVLKEYLTMKAFVAKVVALTAGLGSGIPVGKEGPFVHIASICAAVLSKFMSVFCGVYEQPYYYSDILTVGCAVGVGCCFGTPLGGVLFSIEVTSTYFAVRNYWRGFFAATFSAFVFRVLAVWNKDAVTITALFRTNFRMDFPFDLKELPAFAAIGICCGLLGAVFVYLHRQVMLGVRKHKALSQFLAKHRLLYPGIVTFVIASFTFPPGMGQFMAGELMPREAISTLFDNNTWVKHAGDPESLGQSAVWIHPRVNVVIIIFLFFVMKFWMSIVATTMPIPCGGFMPVFVLGAAFGRLVGEIMAMLFPDGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMMVAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKYTIFVEDIMVRDVKFVSASYTYGELRTLLQTTTVKTLPLVDSKDSMILLGSVERSELQALLQRHLCPERRLRAAQEMARKLSELPYDGKARLAGEGLPGAPPGRPESFAFVDEDEDEDLSGKSELPPSLALHPSTTAPLSPEEPNGPLPGHKQQPEAPEPAGQRPSIFQSLLHCLLGRARPTKKKTTQDSTDLVDNMSPEEIEAWEQEQLSQPVCFDSCCIDQSPFQLVEQTTLHKTHTLFSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSTRKSTGAPPSSAENWNLPEDRPGATGTGDVIAASPETPVPSPSPEPPLSLAPGKVEGELEELELVESPGLEEELADILQGPSLRSTDEEDEDELIL

Target ORF information:

RefSeq Version	NM_000083.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000083.3

ORF Insert Sequence:

ATGGAGCAAT CCCGGTCACA GCAGCGTGGG GGTGAACAAA GCTGGTGGGG TAGTGACCCC 
CAGTACCAGT ATATGCCCTT TGAACACTGC ACCAGCTACG GACTGCCCTC TGAGAATGGG 
GGCCTCCAGC ACAGGCTCCG GAAGGATGCA GGCCCCCGCC ACAACGTCCA CCCCACACAG 
ATTTATGGCC ATCACAAAGA ACAATTCTCA GACAGGGAGC AGGACATAGG GATGCCCAAG 
AAGACAGGCT CCAGTTCTAC CGTGGACAGC AAGGATGAGG ATCACTATTC TAAATGTCAA 
GATTGTATCC ACCGCCTGGG ACAGGTGGTG AGAAGAAAAT TAGGGGAAGA CGGGATCTTT 
CTGGTGCTTC TGGGACTGCT GATGGCTCTG GTCAGCTGGA GCATGGACTA CGTCAGTGCC 
AAAAGCCTTC AGGCCTACAA GTGGTCCTAC GCGCAGATGC AGCCCAGCCT TCCTCTGCAG 
TTCCTGGTCT GGGTCACCTT CCCACTAGTC CTCATCCTCT TCAGCGCCCT CTTCTGCCAC 
CTCATCTCTC CCCAGGCTGT TGGCTCTGGA ATCCCCGAAA TGAAGACAAT ACTTCGTGGG 
GTTGTCCTGA AGGAATACCT CACAATGAAA GCCTTTGTGG CCAAGGTTGT CGCCCTGACT 
GCGGGCCTGG GCAGTGGCAT CCCCGTGGGG AAAGAGGGCC CCTTCGTCCA CATTGCCAGC 
ATCTGTGCTG CTGTCCTCAG CAAATTCATG TCTGTGTTCT GCGGGGTATA TGAGCAGCCA 
TACTACTACT CTGATATCCT GACGGTGGGC TGTGCTGTGG GAGTCGGCTG TTGTTTTGGG 
ACACCACTTG GAGGAGTGCT ATTTAGCATC GAGGTCACCT CCACCTACTT TGCTGTTCGG 
AACTACTGGA GAGGATTCTT TGCAGCCACG TTCAGCGCCT TTGTGTTTCG AGTGCTGGCA 
GTGTGGAACA AGGATGCTGT CACCATCACT GCTCTGTTCA GAACCAATTT CCGAATGGAT 
TTCCCCTTTG ACCTGAAGGA ACTACCAGCT TTTGCTGCCA TCGGGATTTG CTGTGGGCTC 
CTGGGAGCTG TATTTGTGTA TCTGCATCGC CAAGTCATGC TCGGTGTCCG AAAGCACAAG 
GCCCTCAGCC AGTTTCTTGC TAAGCACCGC CTGCTGTATC CTGGAATTGT TACCTTTGTC 
ATTGCCTCAT TCACCTTCCC ACCAGGAATG GGTCAATTCA TGGCTGGAGA GTTGATGCCC 
CGCGAAGCCA TCAGTACTTT GTTTGACAAC AATACATGGG TGAAACACGC GGGTGATCCT 
GAGAGCCTGG GCCAGTCAGC TGTGTGGATT CACCCCCGGG TCAACGTTGT CATCATCATC 
TTTCTCTTCT TCGTCATGAA GTTCTGGATG TCCATCGTGG CCACCACTAT GCCCATACCC 
TGCGGAGGCT TCATGCCTGT GTTTGTGCTA GGAGCTGCAT TTGGAAGGCT GGTAGGAGAA 
ATCATGGCCA TGCTCTTTCC TGATGGTATT TTGTTTGATG ACATCATCTA CAAGATCCTA 
CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA GCGCTGACTG GTGCCGTTTC CCACACAGTC 
TCCACAGCTG TGATTTGCTT CGAATTAACG GGTCAGATTG CTCACATCCT GCCCATGATG 
GTGGCTGTTA TCTTGGCCAA CATGGTGGCC CAGAGCCTGC AGCCCTCTCT CTATGACAGC 
ATCATCCAGG TCAAGAAGCT ACCCTACTTG CCTGACCTTG GCTGGAACCA GCTCAGCAAA 
TATACCATCT TTGTTGAGGA CATCATGGTA CGTGATGTGA AGTTTGTTTC AGCTTCTTAC 
ACATATGGGG AGTTGCGAAC CCTGCTCCAG ACCACCACAG TCAAGACTTT ACCACTGGTT 
GACTCAAAAG ATTCAATGAT CCTGCTGGGC TCGGTGGAGC GGTCGGAACT GCAGGCCCTC 
CTGCAGCGCC ACCTGTGTCC TGAGCGCAGG CTGCGCGCAG CCCAAGAGAT GGCGCGGAAG 
TTGTCGGAGC TGCCTTACGA CGGGAAGGCG CGGCTGGCTG GGGAGGGGCT CCCCGGCGCG 
CCTCCAGGCC GGCCCGAGTC CTTCGCCTTT GTGGATGAGG ATGAGGACGA AGACCTCTCT 
GGCAAGAGCG AGCTTCCTCC TTCCCTTGCT CTCCACCCCT CTACTACTGC CCCTCTGTCC 
CCAGAAGAGC CCAATGGGCC TCTGCCTGGC CACAAACAGC AGCCGGAAGC ACCAGAGCCT 
GCAGGTCAAA GACCCTCCAT CTTCCAGTCC CTGCTTCACT GCTTGCTGGG CAGAGCTCGC 
CCCACAAAGA AGAAAACAAC CCAGGATTCC ACAGATTTAG TGGATAACAT GTCACCTGAA 
GAGATTGAGG CCTGGGAGCA GGAGCAGCTG AGCCAGCCTG TCTGTTTTGA TTCCTGCTGT 
ATTGACCAGT CTCCCTTCCA GCTGGTGGAG CAGACAACCC TGCACAAGAC TCATACCCTG 
TTTTCACTCC TTGGCCTCCA CCTCGCTTAC GTGACCAGCA TGGGGAAGCT CAGGGGCGTC 
CTGGCCCTGG AGGAGCTACA GAAGGCCATT GAGGGGCACA CCAAGTCTGG GGTGCAGCTC 
CGCCCTCCCC TTGCCAGCTT CCGGAACACG ACTTCAACTC GAAAGAGTAC CGGGGCACCT 
CCATCTTCTG CAGAGAACTG GAACCTGCCT GAGGACAGGC CTGGGGCCAC TGGAACAGGG 
GATGTGATTG CTGCCTCCCC AGAGACCCCT GTGCCATCTC CTTCCCCAGA GCCCCCTCTC 
TCCCTGGCCC CAGGCAAGGT AGAGGGCGAG TTGGAGGAGC TGGAGCTGGT GGAGAGTCCA 
GGGCTGGAAG AGGAGCTGGC CGACATCTTG CAGGGCCCCA GCCTGCGATC CACAGACGAG 
GAGGATGAGG ATGAACTGAT CCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCN1 ( XM_017011739.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867228.1 Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu97036
Clone ID Related Accession (Same CDS sequence)	XM_017011739.1
Accession Version	XM_017011739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	chloride channel protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAGAT TGTATCCACC GCCTGGGACA GGTGGTGAGA AGAAAATTAG GGGAAGACGG 
GATCTTTCTG GTGCTTCTGG GACTGCTGAT GGCTCTGGTC AGCTGGAGCA TGGACTACGT 
CAGTGCCAAA AGCCTTCAGG CCTACAAGTG GTCCTACGCG CAGATGCAGC CCAGCCTTCC 
TCTGCAGTTC CTGGTCTGGG TCACCTTCCC ACTAGTCCTC ATCCTCTTCA GCGCCCTCTT 
CTGCCACCTC ATCTCTCCCC AGGCTGTTGG CTCTGGAATC CCCGAAATGA AGACAATACT 
TCGTGGGGTT GTCCTGAAGG AATACCTCAC AATGAAAGCC TTTGTGGCCA AGGTTGTCGC 
CCTGACTGCG GGCCTGGGCA GTGGCATCCC CGTGGGGAAA GAGGAGTGCT ATTTAGCATC 
GAGGTCACCT CCACCTACTT TGCTGTTCGG AACTACTGGA GAGGATTCTT TGCAGCCACG 
TTCAGCGCCT TTGTGTTTCG AGTGCTGGCA GTGTGGAACA AGGATGCTGT CACCATCACT 
GCTCTGTTCA GAACCAATTT CCGAATGGAT TTCCCCTTTG ACCTGAAGGA ACTACCAGCT 
TTTGCTGCCA TCGGGATTTG CTGTGGGCTC CTGGGAGCTG TATTTGTGTA TCTGCATCGC 
CAAGTCATGC TCGGTGTCCG AAAGCACAAG GCCCTCAGCC AGTTTCTTGC TAAGCACCGC 
CTGCTGTATC CTGGAATTGT TACCTTTGTC ATTGCCTCAT TCACCTTCCC ACCAGGAATG 
GGTCAATTCA TGGCTGGAGA GGTCAGCTGT TGGTGGGGCC ACATGTTGAT GCCCCGCGAA 
GCCATCAGTA CTTTGTTTGA CAACAATACA TGGGTGAAAC ACGCGGGTGA TCCTGAGAGC 
CTGGGCCAGT CAGCTGTGTG GATTCACCCC CGGGTCAACG TTGTCATCAT CATCTTTCTC 
TTCTTCGTCA TGAAGTTCTG GATGTCCATC GTGGCCACCA CTATGCCCAT ACCCTGCGGA 
GGCTTCATGC CTGTGTTTGT GCTAGGAGCT GCATTTGGAA GGCTGGTAGG AGAAATCATG 
GCCATGCTCT TTCCTGATGG TATTTTGTTT GATGACATCA TCTACAAGAT CCTACCTGGG 
GGCTATGCAG TAATTGGAGC AGCAGCGCTG ACTGGTGCCG TTTCCCACAC AGTCTCCACA 
GCTGTGATTT GCTTCGAATT AACGGGTCAG ATTGCTCACA TCCTGCCCAT GATGGTGGCT 
GTTATCTTGG CCAACATGGT GGCCCAGAGC CTGCAGCCCT CTCTCTATGA CAGCATCATC 
CAGGTCAAGA AGCTACCCTA CTTGCCTGAC CTTGGCTGGA ACCAGCTCAG CAAATATACC 
ATCTTTGTTG AGGACATCAT GGTACGTGAT GTGAAGTTTG TTTCAGCTTC TTACACATAT 
GGGGAGTTGC GAACCCTGCT CCAGACCACC ACAGTCAAGA CTTTACCACT GGTTGACTCA 
AAAGATTCAA TGATCCTGCT GGGCTCGGTG GAGCGGTCGG AACTGCAGGC CCTCCTGCAG 
CGCCACCTGT GTCCTGAGCG CAGGCTGCGC GCAGCCCAAG AGATGGCGCG GAAGTTGTCG 
GAGCTGCCTT ACGACGGGAA GGCGCGGCTG GCTGGGGAGG GGCTCCCCGG CGCGCCTCCA 
GGCCGGCCCG AGTCCTTCGC CTTTGTGGAT GAGGATGAGG ACGAAGACCT CTCTGGCAAG 
AGCGAGCTTC CTCCTTCCCT TGCTCTCCAC CCCTCTACTA CTGCCCCTCT GTCCCCAGAA 
GAGCCCAATG GGCCTCTGCC TGGCCACAAA CAGCAGCCGG AAGCACCAGA GCCTGCAGGT 
CAAAGACCCT CCATCTTCCA GTCCCTGCTT CACTGCTTGC TGGGCAGAGC TCGCCCCACA 
AAGAAGAAAA CAACCCAGGA TTCCACAGAT TTAGTGGATA ACATGTCACC TGAAGAGATT 
GAGGCCTGGG AGCAGGAGCA GCTGAGCCAG CCTGTCTGTT TTGATTCCTG CTGTATTGAC 
CAGTCTCCCT TCCAGCTGGT GGAGCAGACA ACCCTGCACA AGACTCATAC CCTGTTTTCA 
CTCCTTGGCC TCCACCTCGC TTACGTGACC AGCATGGGGA AGCTCAGGGG CGTCCTGGCC 
CTGGAGGAGC TACAGAAGGC CATTGAGGGG CACACCAAGT CTGGGGTGCA GCTCCGCCCT 
CCCCTTGCCA GCTTCCGGAA CACGACTTCA ACTCGAAAGA GTACCGGGGC ACCTCCATCT 
TCTGCAGAGA ACTGGAACCT GCCTGAGGAC AGGCCTGGGG CCACTGGAAC AGGGGATGTG 
ATTGCTGCCT CCCCAGAGAC CCCTGTGCCA TCTCCTTCCC CAGAGCCCCC TCTCTCCCTG 
GCCCCAGGCA AGGTAGAGGG CGAGTTGGAG GAGCTGGAGC TGGTGGAGAG TCCAGGGCTG 
GAAGAGGAGC TGGCCGACAT CTTGCAGGGC CCCAGCCTGC GATCCACAGA CGAGGAGGAT 
GAGGATGAAC TGATCCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867228.1
CDS	239..2779
Translation	MSRLYPPPGTGGEKKIRGRRDLSGASGTADGSGQLEHGLRQCQKPSGLQVVLRADAAQPSSAVPGLGHLPTSPHPLQRPLLPPHLSPGCWLWNPRNEDNTSWGCPEGIPHNESLCGQGCRPDCGPGQWHPRGERGVLFSIEVTSTYFAVRNYWRGFFAATFSAFVFRVLAVWNKDAVTITALFRTNFRMDFPFDLKELPAFAAIGICCGLLGAVFVYLHRQVMLGVRKHKALSQFLAKHRLLYPGIVTFVIASFTFPPGMGQFMAGEVSCWWGHMLMPREAISTLFDNNTWVKHAGDPESLGQSAVWIHPRVNVVIIIFLFFVMKFWMSIVATTMPIPCGGFMPVFVLGAAFGRLVGEIMAMLFPDGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMMVAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKYTIFVEDIMVRDVKFVSASYTYGELRTLLQTTTVKTLPLVDSKDSMILLGSVERSELQALLQRHLCPERRLRAAQEMARKLSELPYDGKARLAGEGLPGAPPGRPESFAFVDEDEDEDLSGKSELPPSLALHPSTTAPLSPEEPNGPLPGHKQQPEAPEPAGQRPSIFQSLLHCLLGRARPTKKKTTQDSTDLVDNMSPEEIEAWEQEQLSQPVCFDSCCIDQSPFQLVEQTTLHKTHTLFSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSTRKSTGAPPSSAENWNLPEDRPGATGTGDVIAASPETPVPSPSPEPPLSLAPGKVEGELEELELVESPGLEEELADILQGPSLRSTDEEDEDELIL

Target ORF information:

RefSeq Version	XM_017011739.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011739.1

ORF Insert Sequence:

ATGTCAAGAT TGTATCCACC GCCTGGGACA GGTGGTGAGA AGAAAATTAG GGGAAGACGG 
GATCTTTCTG GTGCTTCTGG GACTGCTGAT GGCTCTGGTC AGCTGGAGCA TGGACTACGT 
CAGTGCCAAA AGCCTTCAGG CCTACAAGTG GTCCTACGCG CAGATGCAGC CCAGCCTTCC 
TCTGCAGTTC CTGGTCTGGG TCACCTTCCC ACTAGTCCTC ATCCTCTTCA GCGCCCTCTT 
CTGCCACCTC ATCTCTCCCC AGGCTGTTGG CTCTGGAATC CCCGAAATGA AGACAATACT 
TCGTGGGGTT GTCCTGAAGG AATACCTCAC AATGAAAGCC TTTGTGGCCA AGGTTGTCGC 
CCTGACTGCG GGCCTGGGCA GTGGCATCCC CGTGGGGAAA GAGGAGTGCT ATTTAGCATC 
GAGGTCACCT CCACCTACTT TGCTGTTCGG AACTACTGGA GAGGATTCTT TGCAGCCACG 
TTCAGCGCCT TTGTGTTTCG AGTGCTGGCA GTGTGGAACA AGGATGCTGT CACCATCACT 
GCTCTGTTCA GAACCAATTT CCGAATGGAT TTCCCCTTTG ACCTGAAGGA ACTACCAGCT 
TTTGCTGCCA TCGGGATTTG CTGTGGGCTC CTGGGAGCTG TATTTGTGTA TCTGCATCGC 
CAAGTCATGC TCGGTGTCCG AAAGCACAAG GCCCTCAGCC AGTTTCTTGC TAAGCACCGC 
CTGCTGTATC CTGGAATTGT TACCTTTGTC ATTGCCTCAT TCACCTTCCC ACCAGGAATG 
GGTCAATTCA TGGCTGGAGA GGTCAGCTGT TGGTGGGGCC ACATGTTGAT GCCCCGCGAA 
GCCATCAGTA CTTTGTTTGA CAACAATACA TGGGTGAAAC ACGCGGGTGA TCCTGAGAGC 
CTGGGCCAGT CAGCTGTGTG GATTCACCCC CGGGTCAACG TTGTCATCAT CATCTTTCTC 
TTCTTCGTCA TGAAGTTCTG GATGTCCATC GTGGCCACCA CTATGCCCAT ACCCTGCGGA 
GGCTTCATGC CTGTGTTTGT GCTAGGAGCT GCATTTGGAA GGCTGGTAGG AGAAATCATG 
GCCATGCTCT TTCCTGATGG TATTTTGTTT GATGACATCA TCTACAAGAT CCTACCTGGG 
GGCTATGCAG TAATTGGAGC AGCAGCGCTG ACTGGTGCCG TTTCCCACAC AGTCTCCACA 
GCTGTGATTT GCTTCGAATT AACGGGTCAG ATTGCTCACA TCCTGCCCAT GATGGTGGCT 
GTTATCTTGG CCAACATGGT GGCCCAGAGC CTGCAGCCCT CTCTCTATGA CAGCATCATC 
CAGGTCAAGA AGCTACCCTA CTTGCCTGAC CTTGGCTGGA ACCAGCTCAG CAAATATACC 
ATCTTTGTTG AGGACATCAT GGTACGTGAT GTGAAGTTTG TTTCAGCTTC TTACACATAT 
GGGGAGTTGC GAACCCTGCT CCAGACCACC ACAGTCAAGA CTTTACCACT GGTTGACTCA 
AAAGATTCAA TGATCCTGCT GGGCTCGGTG GAGCGGTCGG AACTGCAGGC CCTCCTGCAG 
CGCCACCTGT GTCCTGAGCG CAGGCTGCGC GCAGCCCAAG AGATGGCGCG GAAGTTGTCG 
GAGCTGCCTT ACGACGGGAA GGCGCGGCTG GCTGGGGAGG GGCTCCCCGG CGCGCCTCCA 
GGCCGGCCCG AGTCCTTCGC CTTTGTGGAT GAGGATGAGG ACGAAGACCT CTCTGGCAAG 
AGCGAGCTTC CTCCTTCCCT TGCTCTCCAC CCCTCTACTA CTGCCCCTCT GTCCCCAGAA 
GAGCCCAATG GGCCTCTGCC TGGCCACAAA CAGCAGCCGG AAGCACCAGA GCCTGCAGGT 
CAAAGACCCT CCATCTTCCA GTCCCTGCTT CACTGCTTGC TGGGCAGAGC TCGCCCCACA 
AAGAAGAAAA CAACCCAGGA TTCCACAGAT TTAGTGGATA ACATGTCACC TGAAGAGATT 
GAGGCCTGGG AGCAGGAGCA GCTGAGCCAG CCTGTCTGTT TTGATTCCTG CTGTATTGAC 
CAGTCTCCCT TCCAGCTGGT GGAGCAGACA ACCCTGCACA AGACTCATAC CCTGTTTTCA 
CTCCTTGGCC TCCACCTCGC TTACGTGACC AGCATGGGGA AGCTCAGGGG CGTCCTGGCC 
CTGGAGGAGC TACAGAAGGC CATTGAGGGG CACACCAAGT CTGGGGTGCA GCTCCGCCCT 
CCCCTTGCCA GCTTCCGGAA CACGACTTCA ACTCGAAAGA GTACCGGGGC ACCTCCATCT 
TCTGCAGAGA ACTGGAACCT GCCTGAGGAC AGGCCTGGGG CCACTGGAAC AGGGGATGTG 
ATTGCTGCCT CCCCAGAGAC CCCTGTGCCA TCTCCTTCCC CAGAGCCCCC TCTCTCCCTG 
GCCCCAGGCA AGGTAGAGGG CGAGTTGGAG GAGCTGGAGC TGGTGGAGAG TCCAGGGCTG 
GAAGAGGAGC TGGCCGACAT CTTGCAGGGC CCCAGCCTGC GATCCACAGA CGAGGAGGAT 
GAGGATGAAC TGATCCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCN1 ( XM_017011740.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867229.1 Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant X2, mRNA.

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CloneID	OHu97037
Clone ID Related Accession (Same CDS sequence)	XM_017011740.1
Accession Version	XM_017011740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	chloride channel protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAGAT TGTATCCACC GCCTGGGACA GGTGGTGAGA AGAAAATTAG GGGAAGACGG 
GATCTTTCTG GTGCTTCTGG GACTGCTGAT GGCTCTGGTC AGCTGGAGCA TGGACTACGT 
CAGTGCCAAA AGCCTTCAGG CCTACAAGTG GTCCTACGCG CAGATGCAGC CCAGCCTTCC 
TCTGCAGTTC CTGGTCTGGG TCACCTTCCC ACTAGTCCTC ATCCTCTTCA GCGCCCTCTT 
CTGCCACCTC ATCTCTCCCC AGGCTGTTGG CTCTGGAATC CCCGAAATGA AGACAATACT 
TCGTGGGGTT GTCCTGAAGG AATACCTCAC AATGAAAGCC TTTGTGGCCA AGGTTGTCGC 
CCTGACTGCG GGCCTGGGCA GTGGCATCCC CGTGGGGAAA GAGGAGTGCT ATTTAGCATC 
GAGGTCACCT CCACCTACTT TGCTGTTCGG AACTACTGGA GAGGATTCTT TGCAGCCACG 
TTCAGCGCCT TTGTGTTTCG AGTGCTGGCA GTGTGGAACA AGGATGCTGT CACCATCACT 
GCTCTGTTCA GAACCAATTT CCGAATGGAT TTCCCCTTTG ACCTGAAGGA ACTACCAGCT 
TTTGCTGCCA TCGGGATTTG CTGTGGGCTC CTGGGAGCTG TATTTGTGTA TCTGCATCGC 
CAAGTCATGC TCGGTGTCCG AAAGCACAAG GCCCTCAGCC AGTTTCTTGC TAAGCACCGC 
CTGCTGTATC CTGGAATTGT TACCTTTGTC ATTGCCTCAT TCACCTTCCC ACCAGGAATG 
GGTCAATTCA TGGCTGGAGA GTTGATGCCC CGCGAAGCCA TCAGTACTTT GTTTGACAAC 
AATACATGGG TGAAACACGC GGGTGATCCT GAGAGCCTGG GCCAGTCAGC TGTGTGGATT 
CACCCCCGGG TCAACGTTGT CATCATCATC TTTCTCTTCT TCGTCATGAA GTTCTGGATG 
TCCATCGTGG CCACCACTAT GCCCATACCC TGCGGAGGCT TCATGCCTGT GTTTGTGCTA 
GGAGCTGCAT TTGGAAGGCT GGTAGGAGAA ATCATGGCCA TGCTCTTTCC TGATGGTATT 
TTGTTTGATG ACATCATCTA CAAGATCCTA CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA 
GCGCTGACTG GTGCCGTTTC CCACACAGTC TCCACAGCTG TGATTTGCTT CGAATTAACG 
GGTCAGATTG CTCACATCCT GCCCATGATG GTGGCTGTTA TCTTGGCCAA CATGGTGGCC 
CAGAGCCTGC AGCCCTCTCT CTATGACAGC ATCATCCAGG TCAAGAAGCT ACCCTACTTG 
CCTGACCTTG GCTGGAACCA GCTCAGCAAA TATACCATCT TTGTTGAGGA CATCATGGTA 
CGTGATGTGA AGTTTGTTTC AGCTTCTTAC ACATATGGGG AGTTGCGAAC CCTGCTCCAG 
ACCACCACAG TCAAGACTTT ACCACTGGTT GACTCAAAAG ATTCAATGAT CCTGCTGGGC 
TCGGTGGAGC GGTCGGAACT GCAGGCCCTC CTGCAGCGCC ACCTGTGTCC TGAGCGCAGG 
CTGCGCGCAG CCCAAGAGAT GGCGCGGAAG TTGTCGGAGC TGCCTTACGA CGGGAAGGCG 
CGGCTGGCTG GGGAGGGGCT CCCCGGCGCG CCTCCAGGCC GGCCCGAGTC CTTCGCCTTT 
GTGGATGAGG ATGAGGACGA AGACCTCTCT GGCAAGAGCG AGCTTCCTCC TTCCCTTGCT 
CTCCACCCCT CTACTACTGC CCCTCTGTCC CCAGAAGAGC CCAATGGGCC TCTGCCTGGC 
CACAAACAGC AGCCGGAAGC ACCAGAGCCT GCAGGTCAAA GACCCTCCAT CTTCCAGTCC 
CTGCTTCACT GCTTGCTGGG CAGAGCTCGC CCCACAAAGA AGAAAACAAC CCAGGATTCC 
ACAGATTTAG TGGATAACAT GTCACCTGAA GAGATTGAGG CCTGGGAGCA GGAGCAGCTG 
AGCCAGCCTG TCTGTTTTGA TTCCTGCTGT ATTGACCAGT CTCCCTTCCA GCTGGTGGAG 
CAGACAACCC TGCACAAGAC TCATACCCTG TTTTCACTCC TTGGCCTCCA CCTCGCTTAC 
GTGACCAGCA TGGGGAAGCT CAGGGGCGTC CTGGCCCTGG AGGAGCTACA GAAGGCCATT 
GAGGGGCACA CCAAGTCTGG GGTGCAGCTC CGCCCTCCCC TTGCCAGCTT CCGGAACACG 
ACTTCAACTC GAAAGAGTAC CGGGGCACCT CCATCTTCTG CAGAGAACTG GAACCTGCCT 
GAGGACAGGC CTGGGGCCAC TGGAACAGGG GATGTGATTG CTGCCTCCCC AGAGACCCCT 
GTGCCATCTC CTTCCCCAGA GCCCCCTCTC TCCCTGGCCC CAGGCAAGGT AGAGGGCGAG 
TTGGAGGAGC TGGAGCTGGT GGAGAGTCCA GGGCTGGAAG AGGAGCTGGC CGACATCTTG 
CAGGGCCCCA GCCTGCGATC CACAGACGAG GAGGATGAGG ATGAACTGAT CCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867229.1
CDS	239..2755
Translation	MSRLYPPPGTGGEKKIRGRRDLSGASGTADGSGQLEHGLRQCQKPSGLQVVLRADAAQPSSAVPGLGHLPTSPHPLQRPLLPPHLSPGCWLWNPRNEDNTSWGCPEGIPHNESLCGQGCRPDCGPGQWHPRGERGVLFSIEVTSTYFAVRNYWRGFFAATFSAFVFRVLAVWNKDAVTITALFRTNFRMDFPFDLKELPAFAAIGICCGLLGAVFVYLHRQVMLGVRKHKALSQFLAKHRLLYPGIVTFVIASFTFPPGMGQFMAGELMPREAISTLFDNNTWVKHAGDPESLGQSAVWIHPRVNVVIIIFLFFVMKFWMSIVATTMPIPCGGFMPVFVLGAAFGRLVGEIMAMLFPDGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMMVAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKYTIFVEDIMVRDVKFVSASYTYGELRTLLQTTTVKTLPLVDSKDSMILLGSVERSELQALLQRHLCPERRLRAAQEMARKLSELPYDGKARLAGEGLPGAPPGRPESFAFVDEDEDEDLSGKSELPPSLALHPSTTAPLSPEEPNGPLPGHKQQPEAPEPAGQRPSIFQSLLHCLLGRARPTKKKTTQDSTDLVDNMSPEEIEAWEQEQLSQPVCFDSCCIDQSPFQLVEQTTLHKTHTLFSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSTRKSTGAPPSSAENWNLPEDRPGATGTGDVIAASPETPVPSPSPEPPLSLAPGKVEGELEELELVESPGLEEELADILQGPSLRSTDEEDEDELIL

Target ORF information:

RefSeq Version	XM_017011740.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride voltage-gated channel 1 (CLCN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011740.1

ORF Insert Sequence:

ATGTCAAGAT TGTATCCACC GCCTGGGACA GGTGGTGAGA AGAAAATTAG GGGAAGACGG 
GATCTTTCTG GTGCTTCTGG GACTGCTGAT GGCTCTGGTC AGCTGGAGCA TGGACTACGT 
CAGTGCCAAA AGCCTTCAGG CCTACAAGTG GTCCTACGCG CAGATGCAGC CCAGCCTTCC 
TCTGCAGTTC CTGGTCTGGG TCACCTTCCC ACTAGTCCTC ATCCTCTTCA GCGCCCTCTT 
CTGCCACCTC ATCTCTCCCC AGGCTGTTGG CTCTGGAATC CCCGAAATGA AGACAATACT 
TCGTGGGGTT GTCCTGAAGG AATACCTCAC AATGAAAGCC TTTGTGGCCA AGGTTGTCGC 
CCTGACTGCG GGCCTGGGCA GTGGCATCCC CGTGGGGAAA GAGGAGTGCT ATTTAGCATC 
GAGGTCACCT CCACCTACTT TGCTGTTCGG AACTACTGGA GAGGATTCTT TGCAGCCACG 
TTCAGCGCCT TTGTGTTTCG AGTGCTGGCA GTGTGGAACA AGGATGCTGT CACCATCACT 
GCTCTGTTCA GAACCAATTT CCGAATGGAT TTCCCCTTTG ACCTGAAGGA ACTACCAGCT 
TTTGCTGCCA TCGGGATTTG CTGTGGGCTC CTGGGAGCTG TATTTGTGTA TCTGCATCGC 
CAAGTCATGC TCGGTGTCCG AAAGCACAAG GCCCTCAGCC AGTTTCTTGC TAAGCACCGC 
CTGCTGTATC CTGGAATTGT TACCTTTGTC ATTGCCTCAT TCACCTTCCC ACCAGGAATG 
GGTCAATTCA TGGCTGGAGA GTTGATGCCC CGCGAAGCCA TCAGTACTTT GTTTGACAAC 
AATACATGGG TGAAACACGC GGGTGATCCT GAGAGCCTGG GCCAGTCAGC TGTGTGGATT 
CACCCCCGGG TCAACGTTGT CATCATCATC TTTCTCTTCT TCGTCATGAA GTTCTGGATG 
TCCATCGTGG CCACCACTAT GCCCATACCC TGCGGAGGCT TCATGCCTGT GTTTGTGCTA 
GGAGCTGCAT TTGGAAGGCT GGTAGGAGAA ATCATGGCCA TGCTCTTTCC TGATGGTATT 
TTGTTTGATG ACATCATCTA CAAGATCCTA CCTGGGGGCT ATGCAGTAAT TGGAGCAGCA 
GCGCTGACTG GTGCCGTTTC CCACACAGTC TCCACAGCTG TGATTTGCTT CGAATTAACG 
GGTCAGATTG CTCACATCCT GCCCATGATG GTGGCTGTTA TCTTGGCCAA CATGGTGGCC 
CAGAGCCTGC AGCCCTCTCT CTATGACAGC ATCATCCAGG TCAAGAAGCT ACCCTACTTG 
CCTGACCTTG GCTGGAACCA GCTCAGCAAA TATACCATCT TTGTTGAGGA CATCATGGTA 
CGTGATGTGA AGTTTGTTTC AGCTTCTTAC ACATATGGGG AGTTGCGAAC CCTGCTCCAG 
ACCACCACAG TCAAGACTTT ACCACTGGTT GACTCAAAAG ATTCAATGAT CCTGCTGGGC 
TCGGTGGAGC GGTCGGAACT GCAGGCCCTC CTGCAGCGCC ACCTGTGTCC TGAGCGCAGG 
CTGCGCGCAG CCCAAGAGAT GGCGCGGAAG TTGTCGGAGC TGCCTTACGA CGGGAAGGCG 
CGGCTGGCTG GGGAGGGGCT CCCCGGCGCG CCTCCAGGCC GGCCCGAGTC CTTCGCCTTT 
GTGGATGAGG ATGAGGACGA AGACCTCTCT GGCAAGAGCG AGCTTCCTCC TTCCCTTGCT 
CTCCACCCCT CTACTACTGC CCCTCTGTCC CCAGAAGAGC CCAATGGGCC TCTGCCTGGC 
CACAAACAGC AGCCGGAAGC ACCAGAGCCT GCAGGTCAAA GACCCTCCAT CTTCCAGTCC 
CTGCTTCACT GCTTGCTGGG CAGAGCTCGC CCCACAAAGA AGAAAACAAC CCAGGATTCC 
ACAGATTTAG TGGATAACAT GTCACCTGAA GAGATTGAGG CCTGGGAGCA GGAGCAGCTG 
AGCCAGCCTG TCTGTTTTGA TTCCTGCTGT ATTGACCAGT CTCCCTTCCA GCTGGTGGAG 
CAGACAACCC TGCACAAGAC TCATACCCTG TTTTCACTCC TTGGCCTCCA CCTCGCTTAC 
GTGACCAGCA TGGGGAAGCT CAGGGGCGTC CTGGCCCTGG AGGAGCTACA GAAGGCCATT 
GAGGGGCACA CCAAGTCTGG GGTGCAGCTC CGCCCTCCCC TTGCCAGCTT CCGGAACACG 
ACTTCAACTC GAAAGAGTAC CGGGGCACCT CCATCTTCTG CAGAGAACTG GAACCTGCCT 
GAGGACAGGC CTGGGGCCAC TGGAACAGGG GATGTGATTG CTGCCTCCCC AGAGACCCCT 
GTGCCATCTC CTTCCCCAGA GCCCCCTCTC TCCCTGGCCC CAGGCAAGGT AGAGGGCGAG 
TTGGAGGAGC TGGAGCTGGT GGAGAGTCCA GGGCTGGAAG AGGAGCTGGC CGACATCTTG 
CAGGGCCCCA GCCTGCGATC CACAGACGAG GAGGATGAGG ATGAACTGAT CCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CLCN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

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FAX

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