CLCA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLCA1
Entrez Gene ID	1179
Full Name	chloride channel accessory 1
Synonyms	CACC,CACC1,CLCRG1,CaCC-1,GOB5,hCLCA1,hCaCC-1
General protein information	Preferred Names chloride channel accessory 1 Names calcium-activated chloride channel regulator 1 CLCA family member 1, chloride channel regulator calcium-activated chloride channel family member 1 calcium-activated chloride channel protein 1 calcium-dependent chloride channel-1 chloride channel regulator 1 chloride channel, calcium activated, family member 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p22.3
Summary	This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine. [provided by RefSeq, Jul 2008].
Disorder MIM:	603906

mRNA and Protein(s)

mRNA	Protein	Name
NM_001285.3	NP_001276.2	calcium-activated chloride channel regulator 1 precursor
NM_001285.4	NP_001276.3	calcium-activated chloride channel regulator 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLCA1	XP_001143250.1
	CLCA1	NP_001028084.1
Mus musculus (house mouse)	Clca3	NP_059502.1
Canis lupus familiaris (dog)	CLCA1	XP_547299.2
Bos taurus (cattle)	CLCA1	NP_001193105.1
Homo sapiens (human)	CLCA1	NP_001276.2
Rattus norvegicus (Norway rat)	Clca1	NP_001100919.1

Gene Ontology
Bibliography

Related articles in PubMed

CLCA1 suppresses colorectal cancer aggressiveness via inhibition of the Wnt/beta-catenin signaling pathway.
Li X, Hu W, Zhou J, Huang Y, Peng J, Yuan Y, Yu J, Zheng S
Cell communication and signaling : CCS15(1)38(2017 Oct)

Modulation of TMEM16A channel activity by the von Willebrand factor type A (VWA) domain of the calcium-activated chloride channel regulator 1 (CLCA1).
Sala-Rabanal M, Yurtsever Z, Berry KN, Nichols CG, Brett TJ
The Journal of biological chemistry292(22)9164-9174(2017 06)

Comparative Proteomics of Ovarian Cancer Aggregate Formation Reveals an Increased Expression of Calcium-activated Chloride Channel Regulator 1 (CLCA1).
Musrap N, Tuccitto A, Karagiannis GS, Saraon P, Batruch I, Diamandis EP
The Journal of biological chemistry290(28)17218-27(2015 Jul)

Secreted CLCA1 modulates TMEM16A to activate Ca(2+)-dependent chloride currents in human cells.
Sala-Rabanal M, Yurtsever Z, Nichols CG, Brett TJ
eLife4(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increase in TMEM16A activity occurred within minutes of exposure to CLCA1 or after a short treatment with nocodazole, consistent with the hypothesis that CLCA1 stabilizes TMEM16A at the cell surface by preventing its internalization.
Title: Modulation of TMEM16A channel activity by the von Willebrand factor type A (VWA) domain of the calcium-activated chloride channel regulator 1 (CLCA1).

inhibitor and siRNA transfection studies demonstrated an apparent effect of CLCA1 on ovarian cancer cell aggregation
Title: Comparative Proteomics of Ovarian Cancer Aggregate Formation Reveals an Increased Expression of Calcium-activated Chloride Channel Regulator 1 (CLCA1).

Results confirmed that the low expression of CLCA1 in CRC was associated with tumorigenesis, early metastasis, and high chromosomal instability. Low expression of CLCA1 predicts recurrence and lower survival.
Title: Low expression of chloride channel accessory 1 predicts a poor prognosis in colorectal cancer.

Data show that calcium-activated chloride channel regulator 1 (CLCA1) and transmembrane protein 16A (TMEM16A) co-localize and physically interact and that CLCA1 increases the level of TMEM16A protein at the cell surface.
Title: Secreted CLCA1 modulates TMEM16A to activate Ca(2+)-dependent chloride currents in human cells.

These findings demonstrate the ability of hCLCA1 to function as a signaling molecule and activate macrophages, central regulators of airway inflammation.
Title: Secreted hCLCA1 is a signaling molecule that activates airway macrophages.

The following CLCA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLCA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu30018	NM_001285.3 Latest version!	Homo sapiens chloride channel accessory 1 (CLCA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu108580	NM_001285.4 Latest version!	Homo sapiens chloride channel accessory 1 (CLCA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLCA1 ( NM_001285.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276.2 Homo sapiens chloride channel accessory 1 (CLCA1), mRNA.

Price & Availability↑

CloneID	OHu30018
Clone ID Related Accession (Same CDS sequence)	NM_001285.3
Accession Version	NM_001285.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	calcium-activated chloride channel regulator 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA428844.1, AF127036.1 and AL122002.16. On Oct 10, 2006 this sequence version replaced NM_001285.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291999.1, AF127036.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCCAT TTAAGAGTTC TGTGTTCATC TTGATTCTTC ACCTTCTAGA AGGGGCCCTG 
AGTAATTCAC TCATTCAGCT GAACAACAAT GGCTATGAAG GCATTGTCGT TGCAATCGAC 
CCCAATGTGC CAGAAGATGA AACACTCATT CAACAAATAA AGGACATGGT GACCCAGGCA 
TCTCTGTATC TGTTTGAAGC TACAGGAAAG CGATTTTATT TCAAAAATGT TGCCATTTTG 
ATTCCTGAAA CATGGAAGAC AAAGGCTGAC TATGTGAGAC CAAAACTTGA GACCTACAAA 
AATGCTGATG TTCTGGTTGC TGAGTCTACT CCTCCAGGTA ATGATGAACC CTACACTGAG 
CAGATGGGCA ACTGTGGAGA GAAGGGTGAA AGGATCCACC TCACTCCTGA TTTCATTGCA 
GGAAAAAAGT TAGCTGAATA TGGACCACAA GGTAGGGCAT TTGTCCATGA GTGGGCTCAT 
CTACGATGGG GAGTATTTGA CGAGTACAAT AATGATGAGA AATTCTACTT ATCCAATGGA 
AGAATACAAG CAGTAAGATG TTCAGCAGGT ATTACTGGTA CAAATGTAGT AAAGAAGTGT 
CAGGGAGGCA GCTGTTACAC CAAAAGATGC ACATTCAATA AAGTAACAGG ACTCTATGAA 
AAAGGATGTG AGTTTGTTCT CCAATCCCGC CAGACGGAGA AGGCTTCTAT AATGTTTGCA 
CAACATGTTG ATTCTATAGT TGAATTCTGT ACAGAACAAA ACCACAACAA AGAAGCTCCA 
AACAAGCAAA ATCAAAAATG CAATCTCCGA AGCACATGGG AAGTGATCCG TGATTCTGAG 
GACTTTAAGA AAACCACTCC TATGACAACA CAGCCACCAA ATCCCACCTT CTCATTGCTG 
CAGATTGGAC AAAGAATTGT GTGTTTAGTC CTTGACAAAT CTGGAAGCAT GGCGACTGGT 
AACCGCCTCA ATCGACTGAA TCAAGCAGGC CAGCTTTTCC TGCTGCAGAC AGTTGAGCTG 
GGGTCCTGGG TTGGGATGGT GACATTTGAC AGTGCTGCCC ATGTACAAAA TGAACTCATA 
CAGATAAACA GTGGCAGTGA CAGGGACACA CTCGCCAAAA GATTACCTGC AGCAGCTTCA 
GGAGGGACGT CCATCTGCAG CGGGCTTCGA TCGGCATTTA CTGTGATTAG GAAGAAATAT 
CCAACTGATG GATCTGAAAT TGTGCTGCTG ACGGATGGGG AAGACAACAC TATAAGTGGG 
TGCTTTAACG AGGTCAAACA AAGTGGTGCC ATCATCCACA CAGTCGCTTT GGGGCCCTCT 
GCAGCTCAAG AACTAGAGGA GCTGTCCAAA ATGACAGGAG GTTTACAGAC ATATGCTTCA 
GATCAAGTTC AGAACAATGG CCTCATTGAT GCTTTTGGGG CCCTTTCATC AGGAAATGGA 
GCTGTCTCTC AGCGCTCCAT CCAGCTTGAG AGTAAGGGAT TAACCCTCCA GAACAGCCAG 
TGGATGAATG GCACAGTGAT CGTGGACAGC ACCGTGGGAA AGGACACTTT GTTTCTTATC 
ACCTGGACAA TGCAGCCTCC CCAAATCCTT CTCTGGGATC CCAGTGGACA GAAGCAAGGT 
GGCTTTGTAG TGGACAAAAA CACCAAAATG GCCTACCTCC AAATCCCAGG CATTGCTAAG 
GTTGGCACTT GGAAATACAG TCTGCAAGCA AGCTCACAAA CCTTGACCCT GACTGTCACG 
TCCCGTGCGT CCAATGCTAC CCTGCCTCCA ATTACAGTGA CTTCCAAAAC GAACAAGGAC 
ACCAGCAAAT TCCCCAGCCC TCTGGTAGTT TATGCAAATA TTCGCCAAGG AGCCTCCCCA 
ATTCTCAGGG CCAGTGTCAC AGCCCTGATT GAATCAGTGA ATGGAAAAAC AGTTACCTTG 
GAACTACTGG ATAATGGAGC AGGTGCTGAT GCTACTAAGG ATGACGGTGT CTACTCAAGG 
TATTTCACAA CTTATGACAC GAATGGTAGA TACAGTGTAA AAGTGCGGGC TCTGGGAGGA 
GTTAACGCAG CCAGACGGAG AGTGATACCC CAGCAGAGTG GAGCACTGTA CATACCTGGC 
TGGATTGAGA ATGATGAAAT ACAATGGAAT CCACCAAGAC CTGAAATTAA TAAGGATGAT 
GTTCAACACA AGCAAGTGTG TTTCAGCAGA ACATCCTCGG GAGGCTCATT TGTGGCTTCT 
GATGTCCCAA ATGCTCCCAT ACCTGATCTC TTCCCACCTG GCCAAATCAC CGACCTGAAG 
GCGGAAATTC ACGGGGGCAG TCTCATTAAT CTGACTTGGA CAGCTCCTGG GGATGATTAT 
GACCATGGAA CAGCTCACAA GTATATCATT CGAATAAGTA CAAGTATTCT TGATCTCAGA 
GACAAGTTCA ATGAATCTCT TCAAGTGAAT ACTACTGCTC TCATCCCAAA GGAAGCCAAC 
TCTGAGGAAG TCTTTTTGTT TAAACCAGAA AACATTACTT TTGAAAATGG CACAGATCTT 
TTCATTGCTA TTCAGGCTGT TGATAAGGTC GATCTGAAAT CAGAAATATC CAACATTGCA 
CGAGTATCTT TGTTTATTCC TCCACAGACT CCGCCAGAGA CACCTAGTCC TGATGAAACG 
TCTGCTCCTT GTCCTAATAT TCATATCAAC AGCACCATTC CTGGCATTCA CATTTTAAAA 
ATTATGTGGA AGTGGATAGG AGAACTGCAG CTGTCAATAG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276.2
CDS	130..2874
Translation	MGPFKSSVFILILHLLEGALSNSLIQLNNNGYEGIVVAIDPNVPEDETLIQQIKDMVTQASLYLFEATGKRFYFKNVAILIPETWKTKADYVRPKLETYKNADVLVAESTPPGNDEPYTEQMGNCGEKGERIHLTPDFIAGKKLAEYGPQGRAFVHEWAHLRWGVFDEYNNDEKFYLSNGRIQAVRCSAGITGTNVVKKCQGGSCYTKRCTFNKVTGLYEKGCEFVLQSRQTEKASIMFAQHVDSIVEFCTEQNHNKEAPNKQNQKCNLRSTWEVIRDSEDFKKTTPMTTQPPNPTFSLLQIGQRIVCLVLDKSGSMATGNRLNRLNQAGQLFLLQTVELGSWVGMVTFDSAAHVQNELIQINSGSDRDTLAKRLPAAASGGTSICSGLRSAFTVIRKKYPTDGSEIVLLTDGEDNTISGCFNEVKQSGAIIHTVALGPSAAQELEELSKMTGGLQTYASDQVQNNGLIDAFGALSSGNGAVSQRSIQLESKGLTLQNSQWMNGTVIVDSTVGKDTLFLITWTMQPPQILLWDPSGQKQGGFVVDKNTKMAYLQIPGIAKVGTWKYSLQASSQTLTLTVTSRASNATLPPITVTSKTNKDTSKFPSPLVVYANIRQGASPILRASVTALIESVNGKTVTLELLDNGAGADATKDDGVYSRYFTTYDTNGRYSVKVRALGGVNAARRRVIPQQSGALYIPGWIENDEIQWNPPRPEINKDDVQHKQVCFSRTSSGGSFVASDVPNAPIPDLFPPGQITDLKAEIHGGSLINLTWTAPGDDYDHGTAHKYIIRISTSILDLRDKFNESLQVNTTALIPKEANSEEVFLFKPENITFENGTDLFIAIQAVDKVDLKSEISNIARVSLFIPPQTPPETPSPDETSAPCPNIHINSTIPGIHILKIMWKWIGELQLSIA

Target ORF information:

RefSeq Version	NM_001285.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride channel accessory 1 (CLCA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001285.3

ORF Insert Sequence:

ATGGGGCCAT TTAAGAGTTC TGTGTTCATC TTGATTCTTC ACCTTCTAGA AGGGGCCCTG 
AGTAATTCAC TCATTCAGCT GAACAACAAT GGCTATGAAG GCATTGTCGT TGCAATCGAC 
CCCAATGTGC CAGAAGATGA AACACTCATT CAACAAATAA AGGACATGGT GACCCAGGCA 
TCTCTGTATC TGTTTGAAGC TACAGGAAAG CGATTTTATT TCAAAAATGT TGCCATTTTG 
ATTCCTGAAA CATGGAAGAC AAAGGCTGAC TATGTGAGAC CAAAACTTGA GACCTACAAA 
AATGCTGATG TTCTGGTTGC TGAGTCTACT CCTCCAGGTA ATGATGAACC CTACACTGAG 
CAGATGGGCA ACTGTGGAGA GAAGGGTGAA AGGATCCACC TCACTCCTGA TTTCATTGCA 
GGAAAAAAGT TAGCTGAATA TGGACCACAA GGTAGGGCAT TTGTCCATGA GTGGGCTCAT 
CTACGATGGG GAGTATTTGA CGAGTACAAT AATGATGAGA AATTCTACTT ATCCAATGGA 
AGAATACAAG CAGTAAGATG TTCAGCAGGT ATTACTGGTA CAAATGTAGT AAAGAAGTGT 
CAGGGAGGCA GCTGTTACAC CAAAAGATGC ACATTCAATA AAGTAACAGG ACTCTATGAA 
AAAGGATGTG AGTTTGTTCT CCAATCCCGC CAGACGGAGA AGGCTTCTAT AATGTTTGCA 
CAACATGTTG ATTCTATAGT TGAATTCTGT ACAGAACAAA ACCACAACAA AGAAGCTCCA 
AACAAGCAAA ATCAAAAATG CAATCTCCGA AGCACATGGG AAGTGATCCG TGATTCTGAG 
GACTTTAAGA AAACCACTCC TATGACAACA CAGCCACCAA ATCCCACCTT CTCATTGCTG 
CAGATTGGAC AAAGAATTGT GTGTTTAGTC CTTGACAAAT CTGGAAGCAT GGCGACTGGT 
AACCGCCTCA ATCGACTGAA TCAAGCAGGC CAGCTTTTCC TGCTGCAGAC AGTTGAGCTG 
GGGTCCTGGG TTGGGATGGT GACATTTGAC AGTGCTGCCC ATGTACAAAA TGAACTCATA 
CAGATAAACA GTGGCAGTGA CAGGGACACA CTCGCCAAAA GATTACCTGC AGCAGCTTCA 
GGAGGGACGT CCATCTGCAG CGGGCTTCGA TCGGCATTTA CTGTGATTAG GAAGAAATAT 
CCAACTGATG GATCTGAAAT TGTGCTGCTG ACGGATGGGG AAGACAACAC TATAAGTGGG 
TGCTTTAACG AGGTCAAACA AAGTGGTGCC ATCATCCACA CAGTCGCTTT GGGGCCCTCT 
GCAGCTCAAG AACTAGAGGA GCTGTCCAAA ATGACAGGAG GTTTACAGAC ATATGCTTCA 
GATCAAGTTC AGAACAATGG CCTCATTGAT GCTTTTGGGG CCCTTTCATC AGGAAATGGA 
GCTGTCTCTC AGCGCTCCAT CCAGCTTGAG AGTAAGGGAT TAACCCTCCA GAACAGCCAG 
TGGATGAATG GCACAGTGAT CGTGGACAGC ACCGTGGGAA AGGACACTTT GTTTCTTATC 
ACCTGGACAA TGCAGCCTCC CCAAATCCTT CTCTGGGATC CCAGTGGACA GAAGCAAGGT 
GGCTTTGTAG TGGACAAAAA CACCAAAATG GCCTACCTCC AAATCCCAGG CATTGCTAAG 
GTTGGCACTT GGAAATACAG TCTGCAAGCA AGCTCACAAA CCTTGACCCT GACTGTCACG 
TCCCGTGCGT CCAATGCTAC CCTGCCTCCA ATTACAGTGA CTTCCAAAAC GAACAAGGAC 
ACCAGCAAAT TCCCCAGCCC TCTGGTAGTT TATGCAAATA TTCGCCAAGG AGCCTCCCCA 
ATTCTCAGGG CCAGTGTCAC AGCCCTGATT GAATCAGTGA ATGGAAAAAC AGTTACCTTG 
GAACTACTGG ATAATGGAGC AGGTGCTGAT GCTACTAAGG ATGACGGTGT CTACTCAAGG 
TATTTCACAA CTTATGACAC GAATGGTAGA TACAGTGTAA AAGTGCGGGC TCTGGGAGGA 
GTTAACGCAG CCAGACGGAG AGTGATACCC CAGCAGAGTG GAGCACTGTA CATACCTGGC 
TGGATTGAGA ATGATGAAAT ACAATGGAAT CCACCAAGAC CTGAAATTAA TAAGGATGAT 
GTTCAACACA AGCAAGTGTG TTTCAGCAGA ACATCCTCGG GAGGCTCATT TGTGGCTTCT 
GATGTCCCAA ATGCTCCCAT ACCTGATCTC TTCCCACCTG GCCAAATCAC CGACCTGAAG 
GCGGAAATTC ACGGGGGCAG TCTCATTAAT CTGACTTGGA CAGCTCCTGG GGATGATTAT 
GACCATGGAA CAGCTCACAA GTATATCATT CGAATAAGTA CAAGTATTCT TGATCTCAGA 
GACAAGTTCA ATGAATCTCT TCAAGTGAAT ACTACTGCTC TCATCCCAAA GGAAGCCAAC 
TCTGAGGAAG TCTTTTTGTT TAAACCAGAA AACATTACTT TTGAAAATGG CACAGATCTT 
TTCATTGCTA TTCAGGCTGT TGATAAGGTC GATCTGAAAT CAGAAATATC CAACATTGCA 
CGAGTATCTT TGTTTATTCC TCCACAGACT CCGCCAGAGA CACCTAGTCC TGATGAAACG 
TCTGCTCCTT GTCCTAATAT TCATATCAAC AGCACCATTC CTGGCATTCA CATTTTAAAA 
ATTATGTGGA AGTGGATAGG AGAACTGCAG CTGTCAATAG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLCA1 ( NM_001285.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276.3 Homo sapiens chloride channel accessory 1 (CLCA1), mRNA.

Price & Availability↑

CloneID	OHu108580
Clone ID Related Accession (Same CDS sequence)	NM_001285.4
Accession Version	NM_001285.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Homo sapiens(human)
Product	calcium-activated chloride channel regulator 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA428844.1, AL122002.16 and AF127036.1. On Jun 28, 2019 this sequence version replaced NM_001285.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291999.1, AF127036.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCCAT TTAAGAGTTC TGTGTTCATC TTGATTCTTC ACCTTCTAGA AGGGGCCCTG 
AGTAATTCAC TCATTCAGCT GAACAACAAT GGCTATGAAG GCATTGTCGT TGCAATCGAC 
CCCAATGTGC CAGAAGATGA AACACTCATT CAACAAATAA AGGACATGGT GACCCAGGCA 
TCTCTGTATC TGCTTGAAGC TACAGGAAAG CGATTTTATT TCAAAAATGT TGCCATTTTG 
ATTCCTGAAA CATGGAAGAC AAAGGCTGAC TATGTGAGAC CAAAACTTGA GACCTACAAA 
AATGCTGATG TTCTGGTTGC TGAGTCTACT CCTCCAGGTA ATGATGAACC CTACACTGAG 
CAGATGGGCA ACTGTGGAGA GAAGGGTGAA AGGATCCACC TCACTCCTGA TTTCATTGCA 
GGAAAAAAGT TAGCTGAATA TGGACCACAA GGTAGGGCAT TTGTCCATGA GTGGGCTCAT 
CTACGATGGG GAGTATTTGA CGAGTACAAT AATGATGAGA AATTCTACTT ATCCAATGGA 
AGAATACAAG CAGTAAGATG TTCAGCAGGT ATTACTGGTA CAAATGTAGT AAAGAAGTGT 
CAGGGAGGCA GCTGTTACAC CAAAAGATGC ACATTCAATA AAGTAACAGG ACTCTATGAA 
AAAGGATGTG AGTTTGTTCT CCAATCCCGC CAGACGGAGA AGGCTTCTAT AATGTTTGCA 
CAACATGTTG ATTCTATAGT TGAATTCTGT ACAGAACAAA ACCACAACAA AGAAGCTCCA 
AACAAGCAAA ATCAAAAATG CAATCTCCGA AGCACATGGG AAGTGATCCG TGATTCTGAG 
GACTTTAAGA AAACCACTCC TATGACAACA CAGCCACCAA ATCCCACCTT CTCATTGCTG 
CAGATTGGAC AAAGAATTGT GTGTTTAGTC CTTGACAAAT CTGGAAGCAT GGCGACTGGT 
AACCGCCTCA ATCGACTGAA TCAAGCAGGC CAGCTTTTCC TGCTGCAGAC AGTTGAGCTG 
GGGTCCTGGG TTGGGATGGT GACATTTGAC AGTGCTGCCC ATGTACAAAA TGAACTCATA 
CAGATAAACA GTGGCAGTGA CAGGGACACA CTCGCCAAAA GATTACCTGC AGCAGCTTCA 
GGAGGGACGT CCATCTGCAG CGGGCTTCGA TCGGCATTTA CTGTGATTAG GAAGAAATAT 
CCAACTGATG GATCTGAAAT TGTGCTGCTG ACGGATGGGG AAGACAACAC TATAAGTGGG 
TGCTTTAACG AGGTCAAACA AAGTGGTGCC ATCATCCACA CAGTCGCTTT GGGGCCCTCT 
GCAGCTCAAG AACTAGAGGA GCTGTCCAAA ATGACAGGAG GTTTACAGAC ATATGCTTCA 
GATCAAGTTC AGAACAATGG CCTCATTGAT GCTTTTGGGG CCCTTTCATC AGGAAATGGA 
GCTGTCTCTC AGCGCTCCAT CCAGCTTGAG AGTAAGGGAT TAACCCTCCA GAACAGCCAG 
TGGATGAATG GCACAGTGAT CGTGGACAGC ACCGTGGGAA AGGACACTTT GTTTCTTATC 
ACCTGGACAA TGCAGCCTCC CCAAATCCTT CTCTGGGATC CCAGTGGACA GAAGCAAGGT 
GGCTTTGTAG TGGACAAAAA CACCAAAATG GCCTACCTCC AAATCCCAGG CATTGCTAAG 
GTTGGCACTT GGAAATACAG TCTGCAAGCA AGCTCACAAA CCTTGACCCT GACTGTCACG 
TCCCGTGCGT CCAATGCTAC CCTGCCTCCA ATTACAGTGA CTTCCAAAAC GAACAAGGAC 
ACCAGCAAAT TCCCCAGCCC TCTGGTAGTT TATGCAAATA TTCGCCAAGG AGCCTCCCCA 
ATTCTCAGGG CCAGTGTCAC AGCCCTGATT GAATCAGTGA ATGGAAAAAC AGTTACCTTG 
GAACTACTGG ATAATGGAGC AGGTGCTGAT GCTACTAAGG ATGACGGTGT CTACTCAAGG 
TATTTCACAA CTTATGACAC GAATGGTAGA TACAGTGTAA AAGTGCGGGC TCTGGGAGGA 
GTTAACGCAG CCAGACGGAG AGTGATACCC CAGCAGAGTG GAGCACTGTA CATACCTGGC 
TGGATTGAGA ATGATGAAAT ACAATGGAAT CCACCAAGAC CTGAAATTAA TAAGGATGAT 
GTTCAACACA AGCAAGTGTG TTTCAGCAGA ACATCCTCGG GAGGCTCATT TGTGGCTTCT 
GATGTCCCAA ATGCTCCCAT ACCTGATCTC TTCCCACCTG GCCAAATCAC CGACCTGAAG 
GCGGAAATTC ACGGGGGCAG TCTCATTAAT CTGACTTGGA CAGCTCCTGG GGATGATTAT 
GACCATGGAA CAGCTCACAA GTATATCATT CGAATAAGTA CAAGTATTCT TGATCTCAGA 
GACAAGTTCA ATGAATCTCT TCAAGTGAAT ACTACTGCTC TCATCCCAAA GGAAGCCAAC 
TCTGAGGAAG TCTTTTTGTT TAAACCAGAA AACATTACTT TTGAAAATGG CACAGATCTT 
TTCATTGCTA TTCAGGCTGT TGATAAGGTC GATCTGAAAT CAGAAATATC CAACATTGCA 
CGAGTATCTT TGTTTATTCC TCCACAGACT CCGCCAGAGA CACCTAGTCC TGATGAAACG 
TCTGCTCCTT GTCCTAATAT TCATATCAAC AGCACCATTC CTGGCATTCA CATTTTAAAA 
ATTATGTGGA AGTGGATAGG AGAACTGCAG CTGTCAATAG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276.3
CDS	46..2790
Translation	MGPFKSSVFILILHLLEGALSNSLIQLNNNGYEGIVVAIDPNVPEDETLIQQIKDMVTQASLYLLEATGKRFYFKNVAILIPETWKTKADYVRPKLETYKNADVLVAESTPPGNDEPYTEQMGNCGEKGERIHLTPDFIAGKKLAEYGPQGRAFVHEWAHLRWGVFDEYNNDEKFYLSNGRIQAVRCSAGITGTNVVKKCQGGSCYTKRCTFNKVTGLYEKGCEFVLQSRQTEKASIMFAQHVDSIVEFCTEQNHNKEAPNKQNQKCNLRSTWEVIRDSEDFKKTTPMTTQPPNPTFSLLQIGQRIVCLVLDKSGSMATGNRLNRLNQAGQLFLLQTVELGSWVGMVTFDSAAHVQNELIQINSGSDRDTLAKRLPAAASGGTSICSGLRSAFTVIRKKYPTDGSEIVLLTDGEDNTISGCFNEVKQSGAIIHTVALGPSAAQELEELSKMTGGLQTYASDQVQNNGLIDAFGALSSGNGAVSQRSIQLESKGLTLQNSQWMNGTVIVDSTVGKDTLFLITWTMQPPQILLWDPSGQKQGGFVVDKNTKMAYLQIPGIAKVGTWKYSLQASSQTLTLTVTSRASNATLPPITVTSKTNKDTSKFPSPLVVYANIRQGASPILRASVTALIESVNGKTVTLELLDNGAGADATKDDGVYSRYFTTYDTNGRYSVKVRALGGVNAARRRVIPQQSGALYIPGWIENDEIQWNPPRPEINKDDVQHKQVCFSRTSSGGSFVASDVPNAPIPDLFPPGQITDLKAEIHGGSLINLTWTAPGDDYDHGTAHKYIIRISTSILDLRDKFNESLQVNTTALIPKEANSEEVFLFKPENITFENGTDLFIAIQAVDKVDLKSEISNIARVSLFIPPQTPPETPSPDETSAPCPNIHINSTIPGIHILKIMWKWIGELQLSIA

Target ORF information:

RefSeq Version	NM_001285.4
Organism	Homo sapiens(human)
Definition	Homo sapiens chloride channel accessory 1 (CLCA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001285.4

ORF Insert Sequence:

ATGGGGCCAT TTAAGAGTTC TGTGTTCATC TTGATTCTTC ACCTTCTAGA AGGGGCCCTG 
AGTAATTCAC TCATTCAGCT GAACAACAAT GGCTATGAAG GCATTGTCGT TGCAATCGAC 
CCCAATGTGC CAGAAGATGA AACACTCATT CAACAAATAA AGGACATGGT GACCCAGGCA 
TCTCTGTATC TGCTTGAAGC TACAGGAAAG CGATTTTATT TCAAAAATGT TGCCATTTTG 
ATTCCTGAAA CATGGAAGAC AAAGGCTGAC TATGTGAGAC CAAAACTTGA GACCTACAAA 
AATGCTGATG TTCTGGTTGC TGAGTCTACT CCTCCAGGTA ATGATGAACC CTACACTGAG 
CAGATGGGCA ACTGTGGAGA GAAGGGTGAA AGGATCCACC TCACTCCTGA TTTCATTGCA 
GGAAAAAAGT TAGCTGAATA TGGACCACAA GGTAGGGCAT TTGTCCATGA GTGGGCTCAT 
CTACGATGGG GAGTATTTGA CGAGTACAAT AATGATGAGA AATTCTACTT ATCCAATGGA 
AGAATACAAG CAGTAAGATG TTCAGCAGGT ATTACTGGTA CAAATGTAGT AAAGAAGTGT 
CAGGGAGGCA GCTGTTACAC CAAAAGATGC ACATTCAATA AAGTAACAGG ACTCTATGAA 
AAAGGATGTG AGTTTGTTCT CCAATCCCGC CAGACGGAGA AGGCTTCTAT AATGTTTGCA 
CAACATGTTG ATTCTATAGT TGAATTCTGT ACAGAACAAA ACCACAACAA AGAAGCTCCA 
AACAAGCAAA ATCAAAAATG CAATCTCCGA AGCACATGGG AAGTGATCCG TGATTCTGAG 
GACTTTAAGA AAACCACTCC TATGACAACA CAGCCACCAA ATCCCACCTT CTCATTGCTG 
CAGATTGGAC AAAGAATTGT GTGTTTAGTC CTTGACAAAT CTGGAAGCAT GGCGACTGGT 
AACCGCCTCA ATCGACTGAA TCAAGCAGGC CAGCTTTTCC TGCTGCAGAC AGTTGAGCTG 
GGGTCCTGGG TTGGGATGGT GACATTTGAC AGTGCTGCCC ATGTACAAAA TGAACTCATA 
CAGATAAACA GTGGCAGTGA CAGGGACACA CTCGCCAAAA GATTACCTGC AGCAGCTTCA 
GGAGGGACGT CCATCTGCAG CGGGCTTCGA TCGGCATTTA CTGTGATTAG GAAGAAATAT 
CCAACTGATG GATCTGAAAT TGTGCTGCTG ACGGATGGGG AAGACAACAC TATAAGTGGG 
TGCTTTAACG AGGTCAAACA AAGTGGTGCC ATCATCCACA CAGTCGCTTT GGGGCCCTCT 
GCAGCTCAAG AACTAGAGGA GCTGTCCAAA ATGACAGGAG GTTTACAGAC ATATGCTTCA 
GATCAAGTTC AGAACAATGG CCTCATTGAT GCTTTTGGGG CCCTTTCATC AGGAAATGGA 
GCTGTCTCTC AGCGCTCCAT CCAGCTTGAG AGTAAGGGAT TAACCCTCCA GAACAGCCAG 
TGGATGAATG GCACAGTGAT CGTGGACAGC ACCGTGGGAA AGGACACTTT GTTTCTTATC 
ACCTGGACAA TGCAGCCTCC CCAAATCCTT CTCTGGGATC CCAGTGGACA GAAGCAAGGT 
GGCTTTGTAG TGGACAAAAA CACCAAAATG GCCTACCTCC AAATCCCAGG CATTGCTAAG 
GTTGGCACTT GGAAATACAG TCTGCAAGCA AGCTCACAAA CCTTGACCCT GACTGTCACG 
TCCCGTGCGT CCAATGCTAC CCTGCCTCCA ATTACAGTGA CTTCCAAAAC GAACAAGGAC 
ACCAGCAAAT TCCCCAGCCC TCTGGTAGTT TATGCAAATA TTCGCCAAGG AGCCTCCCCA 
ATTCTCAGGG CCAGTGTCAC AGCCCTGATT GAATCAGTGA ATGGAAAAAC AGTTACCTTG 
GAACTACTGG ATAATGGAGC AGGTGCTGAT GCTACTAAGG ATGACGGTGT CTACTCAAGG 
TATTTCACAA CTTATGACAC GAATGGTAGA TACAGTGTAA AAGTGCGGGC TCTGGGAGGA 
GTTAACGCAG CCAGACGGAG AGTGATACCC CAGCAGAGTG GAGCACTGTA CATACCTGGC 
TGGATTGAGA ATGATGAAAT ACAATGGAAT CCACCAAGAC CTGAAATTAA TAAGGATGAT 
GTTCAACACA AGCAAGTGTG TTTCAGCAGA ACATCCTCGG GAGGCTCATT TGTGGCTTCT 
GATGTCCCAA ATGCTCCCAT ACCTGATCTC TTCCCACCTG GCCAAATCAC CGACCTGAAG 
GCGGAAATTC ACGGGGGCAG TCTCATTAAT CTGACTTGGA CAGCTCCTGG GGATGATTAT 
GACCATGGAA CAGCTCACAA GTATATCATT CGAATAAGTA CAAGTATTCT TGATCTCAGA 
GACAAGTTCA ATGAATCTCT TCAAGTGAAT ACTACTGCTC TCATCCCAAA GGAAGCCAAC 
TCTGAGGAAG TCTTTTTGTT TAAACCAGAA AACATTACTT TTGAAAATGG CACAGATCTT 
TTCATTGCTA TTCAGGCTGT TGATAAGGTC GATCTGAAAT CAGAAATATC CAACATTGCA 
CGAGTATCTT TGTTTATTCC TCCACAGACT CCGCCAGAGA CACCTAGTCC TGATGAAACG 
TCTGCTCCTT GTCCTAATAT TCATATCAAC AGCACCATTC CTGGCATTCA CATTTTAAAA 
ATTATGTGGA AGTGGATAGG AGAACTGCAG CTGTCAATAG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CLCA1 suppresses colorectal cancer aggressiveness via inhibition of the Wnt/beta-catenin signaling pathway.
Cell communication and signaling : CCS15(1)38(2017 Oct)
Li X,Hu W,Zhou J,Huang Y,Peng J,Yuan Y,Yu J,Zheng S

Modulation of TMEM16A channel activity by the von Willebrand factor type A (VWA) domain of the calcium-activated chloride channel regulator 1 (CLCA1).
The Journal of biological chemistry292(22)9164-9174(2017 06)
Sala-Rabanal M,Yurtsever Z,Berry KN,Nichols CG,Brett TJ

Comparative Proteomics of Ovarian Cancer Aggregate Formation Reveals an Increased Expression of Calcium-activated Chloride Channel Regulator 1 (CLCA1).
The Journal of biological chemistry290(28)17218-27(2015 Jul)
Musrap N,Tuccitto A,Karagiannis GS,Saraon P,Batruch I,Diamandis EP

Secreted CLCA1 modulates TMEM16A to activate Ca(2+)-dependent chloride currents in human cells.
eLife4(2015 Mar)
Sala-Rabanal M,Yurtsever Z,Nichols CG,Brett TJ

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CLCA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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