AP1S1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AP1S1
Entrez Gene ID	1174
Full Name	adaptor related protein complex 1 sigma 1 subunit
Synonyms	AP19,CLAPS1,EKV3,MEDNIK,SIGMA1A
General protein information	Preferred Names adaptor related protein complex 1 sigma 1 subunit Names AP-1 complex subunit sigma-1A HA1 19 kDa subunit adapter-related protein complex 1 sigma-1A subunit adaptor protein complex AP-1 subunit sigma-1A adaptor-related protein complex 1 subunit sigma-1A clathrin assembly protein complex 1 sigma-1A small chain clathrin coat assembly protein AP19 clathrin-associated/assembly/adaptor protein, small 1 (19kD) golgi adaptor HA1/AP1 adaptin sigma-1A subunit sigma1A subunit of AP-1 clathrin adaptor complex sigma1A-adaptin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q22.1
Summary	The protein encoded by this gene is part of the clathrin coat assembly complex which links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. This protein, as well as beta-prime-adaptin, gamma-adaptin, and the medium (mu) chain AP47, form the AP-1 assembly protein complex located at the Golgi vesicle. [provided by RefSeq, Jul 2008].
Disorder MIM:	603531
Disorder Html:	MEDNIK syndrome, 609313 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001283.5	NP_001274.1	AP-1 complex subunit sigma-1A
NM_001283.3	NP_001274.1	AP-1 complex subunit sigma-1A
NM_001283.4	NP_001274.1	AP-1 complex subunit sigma-1A

Pathways from BioSystems

Homologies

Homo sapiens (human)	AP1S1	NP_001274.1
Mus musculus (house mouse)	Ap1s1	NP_031483.1
Rattus norvegicus (Norway rat)	Ap1s1	NP_001101801.2
Xenopus tropicalis (tropical clawed frog)	ap1s1	NP_989338.1
Danio rerio (zebrafish)	zgc:162858	NP_001082809.1
Pan troglodytes (chimpanzee)	AP1S1	XP_001153078.2
Bos taurus (cattle)	AP1S1	NP_001070578.1
Canis lupus familiaris (dog)	AP1S1	XP_003434784.1

Gene Ontology
Bibliography

Related articles in PubMed

MEDNIK syndrome: a novel defect of copper metabolism treatable by zinc acetate therapy.
Martinelli D, Travaglini L, Drouin CA, Ceballos-Picot I, Rizza T, Bertini E, Carrozzo R, Petrini S, de Lonlay P, El Hachem M, Hubert L, Montpetit A, Torre G, Dionisi-Vici C
Brain : a journal of neurology136(Pt 3)872-81(2013 Mar)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Disruption of AP1S1, causing a novel neurocutaneous syndrome, perturbs development of the skin and spinal cord.
Montpetit A, C?t? S, Brustein E, Drouin CA, Lapointe L, Boudreau M, Meloche C, Drouin R, Hudson TJ, Drapeau P, Cossette P
PLoS genetics4(12)e1000296(2008 Dec)

Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection.
Leong WF, Chow VT
Cellular microbiology8(4)565-80(2006 Apr)

An atypical form of erythrokeratodermia variabilis maps to chromosome 7q22.
Saba TG, Montpetit A, Verner A, Rioux P, Hudson TJ, Drouin R, Drouin CA
Human genetics116(3)167-71(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We solved the pathogenetic mechanism of MEDNIK syndrome, demonstrating that AP1S1 regulates intracellular copper machinery mediated by copper-pump proteins
Title: MEDNIK syndrome: a novel defect of copper metabolism treatable by zinc acetate therapy.

A splice mutation in AP1S1 was found in four families with a neurocutaneous syndrome.
Title: Disruption of AP1S1, causing a novel neurocutaneous syndrome, perturbs development of the skin and spinal cord.

Isolation and description of the rat and mouse genes.
Title: AP17 and AP19, the mammalian small chains of the clathrin-associated protein complexes show homology to Yap17p, their putative homolog in yeast.

The following AP1S1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AP1S1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18755	NM_001283.5 Latest version!	Homo sapiens adaptor related protein complex 1 subunit sigma 1 (AP1S1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu18755	NM_001283.3	Homo sapiens adaptor related protein complex 1 sigma 1 subunit (AP1S1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu18755	NM_001283.4	Homo sapiens adaptor related protein complex 1 sigma 1 subunit (AP1S1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AP1S1 ( NM_001283.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274.1 Homo sapiens adaptor related protein complex 1 subunit sigma 1 (AP1S1), mRNA.

Price & Availability↑

CloneID	OHu18755
Clone ID Related Accession (Same CDS sequence)	NM_001283.5 , NM_001283.3 , NM_001283.4
Accession Version	NM_001283.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	AP-1 complex subunit sigma-1A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK312151.1, AL578356.3, CA426442.1 and AC004876.2. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001283.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.116834.1, SRR1163655.331106.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000337619.11/ ENSP00000336666.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGCGGT TCATGCTATT ATTCAGCCGG CAGGGAAAAC TGCGGCTGCA AAAATGGTAC 
CTGGCCACTT CGGACAAGGA ACGGAAGAAG ATGGTGCGCG AGCTCATGCA GGTTGTCCTG 
GCTCGAAAGC CCAAGATGTG CAGCTTCCTG GAGTGGAGGG ACCTCAAAGT TGTCTATAAG 
AGATATGCCA GCCTCTACTT CTGCTGCGCC ATCGAGGGCC AAGACAATGA GCTCATCACA 
CTGGAGCTGA TCCACCGATA CGTGGAGCTC TTAGACAAAT ACTTTGGCAG TGTGTGCGAG 
CTGGACATCA TCTTCAACTT TGAGAAGGCC TACTTCATCC TGGATGAGTT TTTGATGGGG 
GGGGATGTCC AGGACACCTC CAAGAAGAGT GTGCTGAAAG CCATCGAGCA GGCTGACCTA 
CTGCAAGAGG AGGATGAGTC GCCACGGAGT GTGCTGGAGG AGATGGGTTT GGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274.1
CDS	40..516
Translation	MMRFMLLFSRQGKLRLQKWYLATSDKERKKMVRELMQVVLARKPKMCSFLEWRDLKVVYKRYASLYFCCAIEGQDNELITLELIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLMGGDVQDTSKKSVLKAIEQADLLQEEDESPRSVLEEMGLA

Target ORF information:

RefSeq Version	NM_001283.5
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 1 subunit sigma 1 (AP1S1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001283.5

ORF Insert Sequence:

ATGATGCGGT TCATGCTATT ATTCAGCCGG CAGGGAAAAC TGCGGCTGCA AAAATGGTAC 
CTGGCCACTT CGGACAAGGA ACGGAAGAAG ATGGTGCGCG AGCTCATGCA GGTTGTCCTG 
GCTCGAAAGC CCAAGATGTG CAGCTTCCTG GAGTGGAGGG ACCTCAAAGT TGTCTATAAG 
AGATATGCCA GCCTCTACTT CTGCTGCGCC ATCGAGGGCC AAGACAATGA GCTCATCACA 
CTGGAGCTGA TCCACCGATA CGTGGAGCTC TTAGACAAAT ACTTTGGCAG TGTGTGCGAG 
CTGGACATCA TCTTCAACTT TGAGAAGGCC TACTTCATCC TGGATGAGTT TTTGATGGGG 
GGGGATGTCC AGGACACCTC CAAGAAGAGT GTGCTGAAAG CCATCGAGCA GGCTGACCTA 
CTGCAAGAGG AGGATGAGTC GCCACGGAGT GTGCTGGAGG AGATGGGTTT GGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP1S1 ( NM_001283.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274.1 Homo sapiens adaptor related protein complex 1 sigma 1 subunit (AP1S1), mRNA.

Price & Availability↑

CloneID	OHu18755
Clone ID Related Accession (Same CDS sequence)	NM_001283.5 , NM_001283.3 , NM_001283.4
Accession Version	NM_001283.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-29
Organism	Homo sapiens(Human)
Product	AP-1 complex subunit sigma-1A
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI598615.1, AB015319.1 and AC004876.2. This sequence is a reference standard in the RefSeqGene project. On Jun 1, 2007 this sequence version replaced NM_001283.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.116834.1, SRR1163655.420840.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGCGGT TCATGCTATT ATTCAGCCGG CAGGGAAAAC TGCGGCTGCA AAAATGGTAC 
CTGGCCACTT CGGACAAGGA ACGGAAGAAG ATGGTGCGCG AGCTCATGCA GGTTGTCCTG 
GCTCGAAAGC CCAAGATGTG CAGCTTCCTG GAGTGGAGGG ACCTCAAAGT TGTCTATAAG 
AGATATGCCA GCCTCTACTT CTGCTGCGCC ATCGAGGGCC AAGACAATGA GCTCATCACA 
CTGGAGCTGA TCCACCGATA CGTGGAGCTC TTAGACAAAT ACTTTGGCAG TGTGTGCGAG 
CTGGACATCA TCTTCAACTT TGAGAAGGCC TACTTCATCC TGGATGAGTT TTTGATGGGG 
GGGGATGTCC AGGACACCTC CAAGAAGAGT GTGCTGAAAG CCATCGAGCA GGCTGACCTA 
CTGCAAGAGG AGGATGAGTC GCCACGGAGT GTGCTGGAGG AGATGGGTTT GGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274.1
CDS	111..587
Translation	MMRFMLLFSRQGKLRLQKWYLATSDKERKKMVRELMQVVLARKPKMCSFLEWRDLKVVYKRYASLYFCCAIEGQDNELITLELIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLMGGDVQDTSKKSVLKAIEQADLLQEEDESPRSVLEEMGLA

Target ORF information:

RefSeq Version	NM_001283.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens adaptor related protein complex 1 sigma 1 subunit (AP1S1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001283.3

ORF Insert Sequence:

ATGATGCGGT TCATGCTATT ATTCAGCCGG CAGGGAAAAC TGCGGCTGCA AAAATGGTAC 
CTGGCCACTT CGGACAAGGA ACGGAAGAAG ATGGTGCGCG AGCTCATGCA GGTTGTCCTG 
GCTCGAAAGC CCAAGATGTG CAGCTTCCTG GAGTGGAGGG ACCTCAAAGT TGTCTATAAG 
AGATATGCCA GCCTCTACTT CTGCTGCGCC ATCGAGGGCC AAGACAATGA GCTCATCACA 
CTGGAGCTGA TCCACCGATA CGTGGAGCTC TTAGACAAAT ACTTTGGCAG TGTGTGCGAG 
CTGGACATCA TCTTCAACTT TGAGAAGGCC TACTTCATCC TGGATGAGTT TTTGATGGGG 
GGGGATGTCC AGGACACCTC CAAGAAGAGT GTGCTGAAAG CCATCGAGCA GGCTGACCTA 
CTGCAAGAGG AGGATGAGTC GCCACGGAGT GTGCTGGAGG AGATGGGTTT GGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP1S1 ( NM_001283.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274.1 Homo sapiens adaptor related protein complex 1 sigma 1 subunit (AP1S1), mRNA.

Price & Availability↑

CloneID	OHu18755
Clone ID Related Accession (Same CDS sequence)	NM_001283.5 , NM_001283.3 , NM_001283.4
Accession Version	NM_001283.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	AP-1 complex subunit sigma-1A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI598615.1, HY123366.1, AK312151.1, AL578356.3, CA426442.1 and AC004876.2. This sequence is a reference standard in the RefSeqGene project. On Jul 19, 2017 this sequence version replaced NM_001283.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.253516.1, SRR1163655.420840.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGCGGT TCATGCTATT ATTCAGCCGG CAGGGAAAAC TGCGGCTGCA AAAATGGTAC 
CTGGCCACTT CGGACAAGGA ACGGAAGAAG ATGGTGCGCG AGCTCATGCA GGTTGTCCTG 
GCTCGAAAGC CCAAGATGTG CAGCTTCCTG GAGTGGAGGG ACCTCAAAGT TGTCTATAAG 
AGATATGCCA GCCTCTACTT CTGCTGCGCC ATCGAGGGCC AAGACAATGA GCTCATCACA 
CTGGAGCTGA TCCACCGATA CGTGGAGCTC TTAGACAAAT ACTTTGGCAG TGTGTGCGAG 
CTGGACATCA TCTTCAACTT TGAGAAGGCC TACTTCATCC TGGATGAGTT TTTGATGGGG 
GGGGATGTCC AGGACACCTC CAAGAAGAGT GTGCTGAAAG CCATCGAGCA GGCTGACCTA 
CTGCAAGAGG AGGATGAGTC GCCACGGAGT GTGCTGGAGG AGATGGGTTT GGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274.1
CDS	118..594
Translation	MMRFMLLFSRQGKLRLQKWYLATSDKERKKMVRELMQVVLARKPKMCSFLEWRDLKVVYKRYASLYFCCAIEGQDNELITLELIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLMGGDVQDTSKKSVLKAIEQADLLQEEDESPRSVLEEMGLA

Target ORF information:

RefSeq Version	NM_001283.4
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 1 sigma 1 subunit (AP1S1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001283.4

ORF Insert Sequence:

ATGATGCGGT TCATGCTATT ATTCAGCCGG CAGGGAAAAC TGCGGCTGCA AAAATGGTAC 
CTGGCCACTT CGGACAAGGA ACGGAAGAAG ATGGTGCGCG AGCTCATGCA GGTTGTCCTG 
GCTCGAAAGC CCAAGATGTG CAGCTTCCTG GAGTGGAGGG ACCTCAAAGT TGTCTATAAG 
AGATATGCCA GCCTCTACTT CTGCTGCGCC ATCGAGGGCC AAGACAATGA GCTCATCACA 
CTGGAGCTGA TCCACCGATA CGTGGAGCTC TTAGACAAAT ACTTTGGCAG TGTGTGCGAG 
CTGGACATCA TCTTCAACTT TGAGAAGGCC TACTTCATCC TGGATGAGTT TTTGATGGGG 
GGGGATGTCC AGGACACCTC CAAGAAGAGT GTGCTGAAAG CCATCGAGCA GGCTGACCTA 
CTGCAAGAGG AGGATGAGTC GCCACGGAGT GTGCTGGAGG AGATGGGTTT GGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MEDNIK syndrome: a novel defect of copper metabolism treatable by zinc acetate therapy.
Brain : a journal of neurology136(Pt 3)872-81(2013 Mar)
Martinelli D,Travaglini L,Drouin CA,Ceballos-Picot I,Rizza T,Bertini E,Carrozzo R,Petrini S,de Lonlay P,El Hachem M,Hubert L,Montpetit A,Torre G,Dionisi-Vici C

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Disruption of AP1S1, causing a novel neurocutaneous syndrome, perturbs development of the skin and spinal cord.
PLoS genetics4(12)e1000296(2008 Dec)
Montpetit A,C?t? S,Brustein E,Drouin CA,Lapointe L,Boudreau M,Meloche C,Drouin R,Hudson TJ,Drapeau P,Cossette P

Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection.
Cellular microbiology8(4)565-80(2006 Apr)
Leong WF,Chow VT

An atypical form of erythrokeratodermia variabilis maps to chromosome 7q22.
Human genetics116(3)167-71(2005 Feb)
Saba TG,Montpetit A,Verner A,Rioux P,Hudson TJ,Drouin R,Drouin CA

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AP1S1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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