TAGAP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TAGAP
Entrez Gene ID	117289
Full Name	T cell activation RhoGTPase activating protein
Synonyms	ARHGAP47,FKSG15,IDDM21,TAGAP1
General protein information	Preferred Names T cell activation RhoGTPase activating protein Names T-cell activation Rho GTPase-activating protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q25.3
Summary	This gene encodes a member of the Rho GTPase-activator protein superfamily. The encoded protein may function as a Rho GTPase-activating protein. Alterations in this gene may be associated with several diseases, including rheumatoid arthritis, celiac disease, and multiple sclerosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2013].
Disorder MIM:	609667

mRNA and Protein(s)

mRNA	Protein	Name
NM_001278733.1	NP_001265662.1	T-cell activation Rho GTPase-activating protein isoform d
NM_152133.2	NP_687034.1	T-cell activation Rho GTPase-activating protein isoform a
NM_054114.4	NP_473455.2	T-cell activation Rho GTPase-activating protein isoform b
NM_138810.3	NP_620165.1	T-cell activation Rho GTPase-activating protein isoform c
NM_054114.5	NP_473455.2	T-cell activation Rho GTPase-activating protein isoform b
NM_001278733.2	NP_001265662.1	T-cell activation Rho GTPase-activating protein isoform d
NM_138810.4	NP_620165.1	T-cell activation Rho GTPase-activating protein isoform c

Pathways from BioSystems

Homologies

Homo sapiens (human)	TAGAP	NP_473455.2
Pan troglodytes (chimpanzee)	TAGAP	XP_518833.3
	TAGAP	XP_001095675.1
Xenopus tropicalis (tropical clawed frog)	tagap	NP_001072700.1
Bos taurus (cattle)	TAGAP	NP_001070291.1
Danio rerio (zebrafish)	tagapb	NP_001004548.1
Canis lupus familiaris (dog)	TAGAP	XP_541176.2
Mus musculus (house mouse)	Tagap	NP_666080.1
Rattus norvegicus (Norway rat)	Tagap	XP_003748743.1
Gallus gallus (chicken)	TAGAP	XP_419701.3

Gene Ontology
Bibliography

Related articles in PubMed

Identification and in silico analysis of functional SNPs of human TAGAP protein: A comprehensive study.
Arshad M, Bhatti A, John P
PloS one13(1)e0188143(2018)

T cell activation Rho GTPase activating protein (TAGAP) is upregulated in clinical and experimental arthritis.
Arshad M, Bhatti A, John P, Jalil F, Borghese F, Kawalkowska JZ, Williams RO, Clanchy FIL
Cytokine(2017 Oct)

Association of rs1738074 polymorphism of TAGAP gene with susceptibility to multiple sclerosis in the ian population.
Jazaeri A, Vallian S
Neuroscience letters64866-69(2017 May)

Association of LPP and TAGAP Polymorphisms with Celiac Disease Risk: A Meta-Analysis.
Huang SQ, Zhang N, Zhou ZX, Huang CC, Zeng CL, Xiao D, Guo CC, Han YJ, Ye XH, Ye XG, Ou ML, Zhang BH, Liu Y, Zeng EY, Yang G, Jing CX
International journal of environmental research and public health14(2)(2017 02)

The multiple sclerosis susceptibility genes TAGAP and IL2RA are regulated by vitamin D in CD4+ T cells.
Berge T, Leikfoss IS, Brorson IS, Bos SD, Page CM, Gustavsen MW, Bj?lgerud A, Holm?y T, Celius EG, Damoiseaux J, Smolders J, Harbo HF, Spurkland A
Genes and immunity17(2)118-27(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggested that TAGAP rs1738074 polymorphism could be considered as a risk factor in the prevalence of multiple sclerosis in the ian population
Title: Association of rs1738074 polymorphism of TAGAP gene with susceptibility to multiple sclerosis in the ian population.

meta-analysis provides robust estimates that polymorphisms in LPP and TAGAP genes are potential risk factors for celiac disease in Europeans and Americans
Title: Association of LPP and TAGAP Polymorphisms with Celiac Disease Risk: A Meta-Analysis.

IL2RA and TAGAP are novel vitamin D target genes. The vitamin D response is observed in samples from both the multiple sclerosis (MS) patients and controls, and is not dependent on the genotype of MS-associated SNPs in the respective genes.
Title: The multiple sclerosis susceptibility genes TAGAP and IL2RA are regulated by vitamin D in CD4+ T cells.

SNPs in TAGAP are associated with increased risk of candidemia.
Title: Immunochip SNP array identifies novel genetic variants conferring susceptibility to candidaemia.

Colonic expression of TAGAP in Crohn's disease varies according to disease severity and location, being the most elevated in patients with severe disease in the sigmoid colon
Title: T-cell activation Rho GTPase-activating protein expression varies with inflammation location and severity in Crohn's disease.

The following TAGAP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TAGAP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20802	NM_152133.2 Latest version!	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu21226	NM_001278733.2 Latest version!	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu21713	NM_054114.5 Latest version!	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu20790	NM_138810.4 Latest version!	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
View Old Accession Versions >>
OHu21713	NM_054114.4	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu21226	NM_001278733.1	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu20790	NM_138810.3	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TAGAP ( NM_152133.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_687034.1 Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 1, mRNA.

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CloneID	OHu20802
Clone ID Related Accession (Same CDS sequence)	NM_152133.2
Accession Version	NM_152133.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	T-cell activation Rho GTPase-activating protein isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF385430.1 and AL035530.11. On Jul 18, 2013 this sequence version replaced NM_152133.1. Transcript Variant: This variant (1) lacks an exon in the coding region compared to variant 2, resulting in the use of a downstream start codon. The encoded isoform (a) has a shorter N-terminus compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF385430.1, CR997002.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGCTC TGGAGATGCA GGACGAGGAG GACAGAATCG AGGCCCTGAA ACAGGTTGCA 
GATAAGCTCC CCCGGCCCAA CCTCCTGCTA CTCAAGCACT TGGTCTATGT GCTGCACCTC 
ATCAGCAAGA ACTCTGAGGT GAACAGGATG GACTCCAGCA ATCTGGCCAT CTGCATTGGA 
CCCAACATGC TCACCCTGGA GAATGACCAG AGCCTGTCAT TTGAAGCCCA GAAGGACCTG 
AACAACAAGG TGAAGACACT GGTGGAATTC CTCATTGATA ACTGCTTTGA AATATTTGGG 
GAGAACATTC CAGTGCATTC CAGTATCACT TCTGATGACT CCCTGGAGCA CACTGACAGT 
TCAGATGTGT CGACCCTGCA GAATGACTCA GCCTACGACA GCAACGACCC TGATGTGGAA 
TCCAACAGCA GCAGTGGCAT CAGCTCTCCC AGCAGGCAGC CCCAGGTGCC CATGGCCACA 
GCTGCTGGCT TGGATAGCGC GGGCCCACAG GATGCCCGAG AGGTCAGCCC AGAGCCCATT 
GTGAGCACCG TGGCCAGGCT GAAAAGCTCC CTCGCACAGC CCGATAGGAG ATACTCAGAG 
CCCAGCATGC CATCCTCCCA GGAGTGCCTC GAGAGCCGGG TGACAAACCA AACACTAACA 
AAGAGTGAAG GGGACTTCCC CGTGCCCCGG GTAGGCTCTC GTTTGGAAAG TGAGGAGGCT 
GAAGACCCAT TTCCAGAGGA GGTCTTCCCT GCAGTGCAAG GCAAAACCAA GAGGCCGGTG 
GACCTGAAGA TCAAGAACTT GGCCCCGGGT TCGGTGCTCC CGCGGGCACT GGTTCTCAAA 
GCCTTCTCCA GCAGCTCGCT GGACGCGTCC TCTGACAGCT CGCCCGTGGC TTCTCCTTCC 
AGTCCCAAAA GAAATTTCTT CAGCAGACAT CAGTCTTTCA CCACAAAGAC AGAGAAAGGC 
AAGCCCAGCC GAGAAATTAA AAAGCACTCC ATGTCTTTCA CCTTTGCCCC TCACAAAAAA 
GTGCTGACCA AAAACCTCAG CGCGGGCTCT GGGAAATCGC AAGACTTTAC CAGGGACCAC 
GTCCCGAGGG GTGTCAGAAA GGAAAGCCAG CTTGCCGGCC GAATCGTGCA GGAAAATGGG 
TGTGAAACCC ACAACCAAAC AGCCCGCGGC TTCTGCCTGA GACCCCACGC CCTCTCGGTG 
GATGATGTGT TCCAGGGAGC TGACTGGGAG AGGCCTGGAA GCCCACCCTC TTATGAAGAG 
GCCATGCAGG GCCCGGCAGC CAGACTAGTG GCCTCCGAGA GCCAGACCGT GGGGAGCATG 
ACGGTGGGGA GCATGAGGGC GAGGATGCTG GAGGCGCACT GCCTCCTACC CCCTCTTCCA 
CCTGCTCACC ACGTAGAGGA CTCAAGACAC AGGGGCAGCA AAGAGCCACT CCCTGGCCAC 
GGACTCTCTC CCCTGCCTGA GCGATGGAAA CAGAGCAGAA CTGTCCATGC TTCTGGGGAC 
TCTCTGGGGC ACGTGTCTGG CCCAGGGAGA CCTGAGCTCC TCCCGCTGAG GACCGTCTCC 
GAGTCCGTGC AGAGGAATAA GCGGGACTGT CTCGTGCGAC GATGTAGCCA GCCGGTCTTT 
GAGGCTGACC AATTCCAATA TGCCAAAGAA TCGTATATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_687034.1
CDS	700..2361
Translation	MGALEMQDEEDRIEALKQVADKLPRPNLLLLKHLVYVLHLISKNSEVNRMDSSNLAICIGPNMLTLENDQSLSFEAQKDLNNKVKTLVEFLIDNCFEIFGENIPVHSSITSDDSLEHTDSSDVSTLQNDSAYDSNDPDVESNSSSGISSPSRQPQVPMATAAGLDSAGPQDAREVSPEPIVSTVARLKSSLAQPDRRYSEPSMPSSQECLESRVTNQTLTKSEGDFPVPRVGSRLESEEAEDPFPEEVFPAVQGKTKRPVDLKIKNLAPGSVLPRALVLKAFSSSSLDASSDSSPVASPSSPKRNFFSRHQSFTTKTEKGKPSREIKKHSMSFTFAPHKKVLTKNLSAGSGKSQDFTRDHVPRGVRKESQLAGRIVQENGCETHNQTARGFCLRPHALSVDDVFQGADWERPGSPPSYEEAMQGPAARLVASESQTVGSMTVGSMRARMLEAHCLLPPLPPAHHVEDSRHRGSKEPLPGHGLSPLPERWKQSRTVHASGDSLGHVSGPGRPELLPLRTVSESVQRNKRDCLVRRCSQPVFEADQFQYAKESYI

Target ORF information:

RefSeq Version	NM_152133.2
Organism	Homo sapiens(human)
Definition	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152133.2

ORF Insert Sequence:

ATGGGTGCTC TGGAGATGCA GGACGAGGAG GACAGAATCG AGGCCCTGAA ACAGGTTGCA 
GATAAGCTCC CCCGGCCCAA CCTCCTGCTA CTCAAGCACT TGGTCTATGT GCTGCACCTC 
ATCAGCAAGA ACTCTGAGGT GAACAGGATG GACTCCAGCA ATCTGGCCAT CTGCATTGGA 
CCCAACATGC TCACCCTGGA GAATGACCAG AGCCTGTCAT TTGAAGCCCA GAAGGACCTG 
AACAACAAGG TGAAGACACT GGTGGAATTC CTCATTGATA ACTGCTTTGA AATATTTGGG 
GAGAACATTC CAGTGCATTC CAGTATCACT TCTGATGACT CCCTGGAGCA CACTGACAGT 
TCAGATGTGT CGACCCTGCA GAATGACTCA GCCTACGACA GCAACGACCC TGATGTGGAA 
TCCAACAGCA GCAGTGGCAT CAGCTCTCCC AGCAGGCAGC CCCAGGTGCC CATGGCCACA 
GCTGCTGGCT TGGATAGCGC GGGCCCACAG GATGCCCGAG AGGTCAGCCC AGAGCCCATT 
GTGAGCACCG TGGCCAGGCT GAAAAGCTCC CTCGCACAGC CCGATAGGAG ATACTCAGAG 
CCCAGCATGC CATCCTCCCA GGAGTGCCTC GAGAGCCGGG TGACAAACCA AACACTAACA 
AAGAGTGAAG GGGACTTCCC CGTGCCCCGG GTAGGCTCTC GTTTGGAAAG TGAGGAGGCT 
GAAGACCCAT TTCCAGAGGA GGTCTTCCCT GCAGTGCAAG GCAAAACCAA GAGGCCGGTG 
GACCTGAAGA TCAAGAACTT GGCCCCGGGT TCGGTGCTCC CGCGGGCACT GGTTCTCAAA 
GCCTTCTCCA GCAGCTCGCT GGACGCGTCC TCTGACAGCT CGCCCGTGGC TTCTCCTTCC 
AGTCCCAAAA GAAATTTCTT CAGCAGACAT CAGTCTTTCA CCACAAAGAC AGAGAAAGGC 
AAGCCCAGCC GAGAAATTAA AAAGCACTCC ATGTCTTTCA CCTTTGCCCC TCACAAAAAA 
GTGCTGACCA AAAACCTCAG CGCGGGCTCT GGGAAATCGC AAGACTTTAC CAGGGACCAC 
GTCCCGAGGG GTGTCAGAAA GGAAAGCCAG CTTGCCGGCC GAATCGTGCA GGAAAATGGG 
TGTGAAACCC ACAACCAAAC AGCCCGCGGC TTCTGCCTGA GACCCCACGC CCTCTCGGTG 
GATGATGTGT TCCAGGGAGC TGACTGGGAG AGGCCTGGAA GCCCACCCTC TTATGAAGAG 
GCCATGCAGG GCCCGGCAGC CAGACTAGTG GCCTCCGAGA GCCAGACCGT GGGGAGCATG 
ACGGTGGGGA GCATGAGGGC GAGGATGCTG GAGGCGCACT GCCTCCTACC CCCTCTTCCA 
CCTGCTCACC ACGTAGAGGA CTCAAGACAC AGGGGCAGCA AAGAGCCACT CCCTGGCCAC 
GGACTCTCTC CCCTGCCTGA GCGATGGAAA CAGAGCAGAA CTGTCCATGC TTCTGGGGAC 
TCTCTGGGGC ACGTGTCTGG CCCAGGGAGA CCTGAGCTCC TCCCGCTGAG GACCGTCTCC 
GAGTCCGTGC AGAGGAATAA GCGGGACTGT CTCGTGCGAC GATGTAGCCA GCCGGTCTTT 
GAGGCTGACC AATTCCAATA TGCCAAAGAA TCGTATATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAGAP ( NM_001278733.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265662.1 Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu21226
Clone ID Related Accession (Same CDS sequence)	NM_001278733.1 , NM_001278733.2
Accession Version	NM_001278733.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	T-cell activation Rho GTPase-activating protein isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA819174.1, AF385429.1 and AL035530.11. Transcript Variant: This variant (4) uses an alternate 5'-most exon, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 2. The encoded isoform (d) has a shorter N-terminus, compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR982931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAGGC TCTCCCCTGC ATCAGATTTT TCTGGGGCTT TGGAGACAGA CTTGAAAGCA 
TCGCTATTTG ATCAGCCCTT GTCAATTATC TGCGGTGACA GTGACACACT CCCCAGACCC 
ATCCAGGACA TTCTCACTAT TCTATGCCTT AAAGGCCCTT CAACGGAAGG GATATTCAGG 
AGAGCAGCCA ACGAGAAAGC CCGTAAGGAG CTGAAGGAGG AGCTCAACTC TGGGGATGCG 
GTGGATCTGG AGAGGCTCCC CGTGCACCTC CTCGCTGTGG TCTTTAAGGA CTTCCTCAGA 
AGTATCCCCC GGAAGCTACT TTCAAGCGAC CTCTTTGAGG AGTGGATGGG TGCTCTGGAG 
ATGCAGGACG AGGAGGACAG AATCGAGGCC CTGAAACAGG TTGCAGATAA GCTCCCCCGG 
CCCAACCTCC TGCTACTCAA GCACTTGGTC TATGTGCTGC ACCTCATCAG CAAGAACTCT 
GAGGTGAACA GGATGGACTC CAGCAATCTG GCCATCTGCA TTGGACCCAA CATGCTCACC 
CTGGAGAATG ACCAGAGCCT GTCATTTGAA GCCCAGAAGG ACCTGAACAA CAAGGTGAAG 
ACACTGGTGG AATTCCTCAT TGATAACTGC TTTGAAATAT TTGGGGAGAA CATTCCAGTG 
CATTCCAGTA TCACTTCTGA TGACTCCCTG GAGCACACTG ACAGTTCAGA TGTGTCGACC 
CTGCAGAATG ACTCAGCCTA CGACAGCAAC GACCCTGATG TGGAATCCAA CAGCAGCAGT 
GGCATCAGCT CTCCCAGCAG GCAGCCCCAG GTGCCCATGG CCACAGCTGC TGGCTTGGAT 
AGCGCGGGCC CACAGGATGC CCGAGAGGTC AGCCCAGAGC CCATTGTGAG CACCGTGGCC 
AGGCTGAAAA GCTCCCTCGC ACAGCCCGAT AGGAGATACT CAGAGCCCAG CATGCCATCC 
TCCCAGGAGT GCCTCGAGAG CCGGGTGACA AACCAAACAC TAACAAAGAG TGAAGGGGAC 
TTCCCCGTGC CCCGGGTAGG CTCTCGTTTG GAAAGTGAGG AGGCTGAAGA CCCATTTCCA 
GAGGAGGTCT TCCCTGCAGT GCAAGGCAAA ACCAAGAGGC CGGTGGACCT GAAGATCAAG 
AACTTGGCCC CGGGTTCGGT GCTCCCGCGG GCACTGGTTC TCAAAGCCTT CTCCAGCAGC 
TCGCTGGACG CGTCCTCTGA CAGCTCGCCC GTGGCTTCTC CTTCCAGTCC CAAAAGAAAT 
TTCTTCAGCA GACATCAGTC TTTCACCACA AAGACAGAGA AAGGCAAGCC CAGCCGAGAA 
ATTAAAAAGC ACTCCATGTC TTTCACCTTT GCCCCTCACA AAAAAGTGCT GACCAAAAAC 
CTCAGCGCGG GCTCTGGGAA ATCGCAAGAC TTTACCAGGG ACCACGTCCC GAGGGGTGTC 
AGAAAGGAAA GCCAGCTTGC CGGCCGAATC GTGCAGGAAA ATGGGTGTGA AACCCACAAC 
CAAACAGCCC GCGGCTTCTG CCTGAGACCC CACGCCCTCT CGGTGGATGA TGTGTTCCAG 
GGAGCTGACT GGGAGAGGCC TGGAAGCCCA CCCTCTTATG AAGAGGCCAT GCAGGGCCCG 
GCAGCCAGAC TAGTGGCCTC CGAGAGCCAG ACCGTGGGGA GCATGACGGT GGGGAGCATG 
AGGGCGAGGA TGCTGGAGGC GCACTGCCTC CTACCCCCTC TTCCACCTGC TCACCACGTA 
GAGGACTCAA GACACAGGGG CAGCAAAGAG CCACTCCCTG GCCACGGACT CTCTCCCCTG 
CCTGAGCGAT GGAAACAGAG CAGAACTGTC CATGCTTCTG GGGACTCTCT GGGGCACGTG 
TCTGGCCCAG GGAGACCTGA GCTCCTCCCG CTGAGGACCG TCTCCGAGTC CGTGCAGAGG 
AATAAGCGGG ACTGTCTCGT GCGACGATGT AGCCAGCCGG TCTTTGAGGC TGACCAATTC 
CAATATGCCA AAGAATCGTA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265662.1
CDS	151..2157
Translation	MRRLSPASDFSGALETDLKASLFDQPLSIICGDSDTLPRPIQDILTILCLKGPSTEGIFRRAANEKARKELKEELNSGDAVDLERLPVHLLAVVFKDFLRSIPRKLLSSDLFEEWMGALEMQDEEDRIEALKQVADKLPRPNLLLLKHLVYVLHLISKNSEVNRMDSSNLAICIGPNMLTLENDQSLSFEAQKDLNNKVKTLVEFLIDNCFEIFGENIPVHSSITSDDSLEHTDSSDVSTLQNDSAYDSNDPDVESNSSSGISSPSRQPQVPMATAAGLDSAGPQDAREVSPEPIVSTVARLKSSLAQPDRRYSEPSMPSSQECLESRVTNQTLTKSEGDFPVPRVGSRLESEEAEDPFPEEVFPAVQGKTKRPVDLKIKNLAPGSVLPRALVLKAFSSSSLDASSDSSPVASPSSPKRNFFSRHQSFTTKTEKGKPSREIKKHSMSFTFAPHKKVLTKNLSAGSGKSQDFTRDHVPRGVRKESQLAGRIVQENGCETHNQTARGFCLRPHALSVDDVFQGADWERPGSPPSYEEAMQGPAARLVASESQTVGSMTVGSMRARMLEAHCLLPPLPPAHHVEDSRHRGSKEPLPGHGLSPLPERWKQSRTVHASGDSLGHVSGPGRPELLPLRTVSESVQRNKRDCLVRRCSQPVFEADQFQYAKESYI

Target ORF information:

RefSeq Version	NM_001278733.1
Organism	Homo sapiens(human)
Definition	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278733.1

ORF Insert Sequence:

ATGAGAAGGC TCTCCCCTGC ATCAGATTTT TCTGGGGCTT TGGAGACAGA CTTGAAAGCA 
TCGCTATTTG ATCAGCCCTT GTCAATTATC TGCGGTGACA GTGACACACT CCCCAGACCC 
ATCCAGGACA TTCTCACTAT TCTATGCCTT AAAGGCCCTT CAACGGAAGG GATATTCAGG 
AGAGCAGCCA ACGAGAAAGC CCGTAAGGAG CTGAAGGAGG AGCTCAACTC TGGGGATGCG 
GTGGATCTGG AGAGGCTCCC CGTGCACCTC CTCGCTGTGG TCTTTAAGGA CTTCCTCAGA 
AGTATCCCCC GGAAGCTACT TTCAAGCGAC CTCTTTGAGG AGTGGATGGG TGCTCTGGAG 
ATGCAGGACG AGGAGGACAG AATCGAGGCC CTGAAACAGG TTGCAGATAA GCTCCCCCGG 
CCCAACCTCC TGCTACTCAA GCACTTGGTC TATGTGCTGC ACCTCATCAG CAAGAACTCT 
GAGGTGAACA GGATGGACTC CAGCAATCTG GCCATCTGCA TTGGACCCAA CATGCTCACC 
CTGGAGAATG ACCAGAGCCT GTCATTTGAA GCCCAGAAGG ACCTGAACAA CAAGGTGAAG 
ACACTGGTGG AATTCCTCAT TGATAACTGC TTTGAAATAT TTGGGGAGAA CATTCCAGTG 
CATTCCAGTA TCACTTCTGA TGACTCCCTG GAGCACACTG ACAGTTCAGA TGTGTCGACC 
CTGCAGAATG ACTCAGCCTA CGACAGCAAC GACCCTGATG TGGAATCCAA CAGCAGCAGT 
GGCATCAGCT CTCCCAGCAG GCAGCCCCAG GTGCCCATGG CCACAGCTGC TGGCTTGGAT 
AGCGCGGGCC CACAGGATGC CCGAGAGGTC AGCCCAGAGC CCATTGTGAG CACCGTGGCC 
AGGCTGAAAA GCTCCCTCGC ACAGCCCGAT AGGAGATACT CAGAGCCCAG CATGCCATCC 
TCCCAGGAGT GCCTCGAGAG CCGGGTGACA AACCAAACAC TAACAAAGAG TGAAGGGGAC 
TTCCCCGTGC CCCGGGTAGG CTCTCGTTTG GAAAGTGAGG AGGCTGAAGA CCCATTTCCA 
GAGGAGGTCT TCCCTGCAGT GCAAGGCAAA ACCAAGAGGC CGGTGGACCT GAAGATCAAG 
AACTTGGCCC CGGGTTCGGT GCTCCCGCGG GCACTGGTTC TCAAAGCCTT CTCCAGCAGC 
TCGCTGGACG CGTCCTCTGA CAGCTCGCCC GTGGCTTCTC CTTCCAGTCC CAAAAGAAAT 
TTCTTCAGCA GACATCAGTC TTTCACCACA AAGACAGAGA AAGGCAAGCC CAGCCGAGAA 
ATTAAAAAGC ACTCCATGTC TTTCACCTTT GCCCCTCACA AAAAAGTGCT GACCAAAAAC 
CTCAGCGCGG GCTCTGGGAA ATCGCAAGAC TTTACCAGGG ACCACGTCCC GAGGGGTGTC 
AGAAAGGAAA GCCAGCTTGC CGGCCGAATC GTGCAGGAAA ATGGGTGTGA AACCCACAAC 
CAAACAGCCC GCGGCTTCTG CCTGAGACCC CACGCCCTCT CGGTGGATGA TGTGTTCCAG 
GGAGCTGACT GGGAGAGGCC TGGAAGCCCA CCCTCTTATG AAGAGGCCAT GCAGGGCCCG 
GCAGCCAGAC TAGTGGCCTC CGAGAGCCAG ACCGTGGGGA GCATGACGGT GGGGAGCATG 
AGGGCGAGGA TGCTGGAGGC GCACTGCCTC CTACCCCCTC TTCCACCTGC TCACCACGTA 
GAGGACTCAA GACACAGGGG CAGCAAAGAG CCACTCCCTG GCCACGGACT CTCTCCCCTG 
CCTGAGCGAT GGAAACAGAG CAGAACTGTC CATGCTTCTG GGGACTCTCT GGGGCACGTG 
TCTGGCCCAG GGAGACCTGA GCTCCTCCCG CTGAGGACCG TCTCCGAGTC CGTGCAGAGG 
AATAAGCGGG ACTGTCTCGT GCGACGATGT AGCCAGCCGG TCTTTGAGGC TGACCAATTC 
CAATATGCCA AAGAATCGTA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAGAP ( NM_001278733.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265662.1 Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 4, mRNA.

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CloneID	OHu21226
Clone ID Related Accession (Same CDS sequence)	NM_001278733.1 , NM_001278733.2
Accession Version	NM_001278733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	T-cell activation Rho GTPase-activating protein isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA819174.1, AF385429.1 and AL035530.11. On Jun 2, 2019 this sequence version replaced NM_001278733.1. Transcript Variant: This variant (4) uses an alternate 5'-most exon, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 2. The encoded isoform (d) has a shorter N-terminus, compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR982931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAGGC TCTCCCCTGC ATCAGATTTT TCTGGGGCTT TGGAGACAGA CTTGAAAGCA 
TCGCTATTTG ATCAGCCCTT GTCAATTATC TGCGGTGACA GTGACACACT CCCCAGACCC 
ATCCAGGACA TTCTCACTAT TCTATGCCTT AAAGGCCCTT CAACGGAAGG GATATTCAGG 
AGAGCAGCCA ACGAGAAAGC CCGTAAGGAG CTGAAGGAGG AGCTCAACTC TGGGGATGCG 
GTGGATCTGG AGAGGCTCCC CGTGCACCTC CTCGCTGTGG TCTTTAAGGA CTTCCTCAGA 
AGTATCCCCC GGAAGCTACT TTCAAGCGAC CTCTTTGAGG AGTGGATGGG TGCTCTGGAG 
ATGCAGGACG AGGAGGACAG AATCGAGGCC CTGAAACAGG TTGCAGATAA GCTCCCCCGG 
CCCAACCTCC TGCTACTCAA GCACTTGGTC TATGTGCTGC ACCTCATCAG CAAGAACTCT 
GAGGTGAACA GGATGGACTC CAGCAATCTG GCCATCTGCA TTGGACCCAA CATGCTCACC 
CTGGAGAATG ACCAGAGCCT GTCATTTGAA GCCCAGAAGG ACCTGAACAA CAAGGTGAAG 
ACACTGGTGG AATTCCTCAT TGATAACTGC TTTGAAATAT TTGGGGAGAA CATTCCAGTG 
CATTCCAGTA TCACTTCTGA TGACTCCCTG GAGCACACTG ACAGTTCAGA TGTGTCGACC 
CTGCAGAATG ACTCAGCCTA CGACAGCAAC GACCCTGATG TGGAATCCAA CAGCAGCAGT 
GGCATCAGCT CTCCCAGCAG GCAGCCCCAG GTGCCCATGG CCACAGCTGC TGGCTTGGAT 
AGCGCGGGCC CACAGGATGC CCGAGAGGTC AGCCCAGAGC CCATTGTGAG CACCGTGGCC 
AGGCTGAAAA GCTCCCTCGC ACAGCCCGAT AGGAGATACT CAGAGCCCAG CATGCCATCC 
TCCCAGGAGT GCCTCGAGAG CCGGGTGACA AACCAAACAC TAACAAAGAG TGAAGGGGAC 
TTCCCCGTGC CCCGGGTAGG CTCTCGTTTG GAAAGTGAGG AGGCTGAAGA CCCATTTCCA 
GAGGAGGTCT TCCCTGCAGT GCAAGGCAAA ACCAAGAGGC CGGTGGACCT GAAGATCAAG 
AACTTGGCCC CGGGTTCGGT GCTCCCGCGG GCACTGGTTC TCAAAGCCTT CTCCAGCAGC 
TCGCTGGACG CGTCCTCTGA CAGCTCGCCC GTGGCTTCTC CTTCCAGTCC CAAAAGAAAT 
TTCTTCAGCA GACATCAGTC TTTCACCACA AAGACAGAGA AAGGCAAGCC CAGCCGAGAA 
ATTAAAAAGC ACTCCATGTC TTTCACCTTT GCCCCTCACA AAAAAGTGCT GACCAAAAAC 
CTCAGCGCGG GCTCTGGGAA ATCGCAAGAC TTTACCAGGG ACCACGTCCC GAGGGGTGTC 
AGAAAGGAAA GCCAGCTTGC CGGCCGAATC GTGCAGGAAA ATGGGTGTGA AACCCACAAC 
CAAACAGCCC GCGGCTTCTG CCTGAGACCC CACGCCCTCT CGGTGGATGA TGTGTTCCAG 
GGAGCTGACT GGGAGAGGCC TGGAAGCCCA CCCTCTTATG AAGAGGCCAT GCAGGGCCCG 
GCAGCCAGAC TAGTGGCCTC CGAGAGCCAG ACCGTGGGGA GCATGACGGT GGGGAGCATG 
AGGGCGAGGA TGCTGGAGGC GCACTGCCTC CTACCCCCTC TTCCACCTGC TCACCACGTA 
GAGGACTCAA GACACAGGGG CAGCAAAGAG CCACTCCCTG GCCACGGACT CTCTCCCCTG 
CCTGAGCGAT GGAAACAGAG CAGAACTGTC CATGCTTCTG GGGACTCTCT GGGGCACGTG 
TCTGGCCCAG GGAGACCTGA GCTCCTCCCG CTGAGGACCG TCTCCGAGTC CGTGCAGAGG 
AATAAGCGGG ACTGTCTCGT GCGACGATGT AGCCAGCCGG TCTTTGAGGC TGACCAATTC 
CAATATGCCA AAGAATCGTA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265662.1
CDS	151..2157
Translation	MRRLSPASDFSGALETDLKASLFDQPLSIICGDSDTLPRPIQDILTILCLKGPSTEGIFRRAANEKARKELKEELNSGDAVDLERLPVHLLAVVFKDFLRSIPRKLLSSDLFEEWMGALEMQDEEDRIEALKQVADKLPRPNLLLLKHLVYVLHLISKNSEVNRMDSSNLAICIGPNMLTLENDQSLSFEAQKDLNNKVKTLVEFLIDNCFEIFGENIPVHSSITSDDSLEHTDSSDVSTLQNDSAYDSNDPDVESNSSSGISSPSRQPQVPMATAAGLDSAGPQDAREVSPEPIVSTVARLKSSLAQPDRRYSEPSMPSSQECLESRVTNQTLTKSEGDFPVPRVGSRLESEEAEDPFPEEVFPAVQGKTKRPVDLKIKNLAPGSVLPRALVLKAFSSSSLDASSDSSPVASPSSPKRNFFSRHQSFTTKTEKGKPSREIKKHSMSFTFAPHKKVLTKNLSAGSGKSQDFTRDHVPRGVRKESQLAGRIVQENGCETHNQTARGFCLRPHALSVDDVFQGADWERPGSPPSYEEAMQGPAARLVASESQTVGSMTVGSMRARMLEAHCLLPPLPPAHHVEDSRHRGSKEPLPGHGLSPLPERWKQSRTVHASGDSLGHVSGPGRPELLPLRTVSESVQRNKRDCLVRRCSQPVFEADQFQYAKESYI

Target ORF information:

RefSeq Version	NM_001278733.2
Organism	Homo sapiens(human)
Definition	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278733.2

ORF Insert Sequence:

ATGAGAAGGC TCTCCCCTGC ATCAGATTTT TCTGGGGCTT TGGAGACAGA CTTGAAAGCA 
TCGCTATTTG ATCAGCCCTT GTCAATTATC TGCGGTGACA GTGACACACT CCCCAGACCC 
ATCCAGGACA TTCTCACTAT TCTATGCCTT AAAGGCCCTT CAACGGAAGG GATATTCAGG 
AGAGCAGCCA ACGAGAAAGC CCGTAAGGAG CTGAAGGAGG AGCTCAACTC TGGGGATGCG 
GTGGATCTGG AGAGGCTCCC CGTGCACCTC CTCGCTGTGG TCTTTAAGGA CTTCCTCAGA 
AGTATCCCCC GGAAGCTACT TTCAAGCGAC CTCTTTGAGG AGTGGATGGG TGCTCTGGAG 
ATGCAGGACG AGGAGGACAG AATCGAGGCC CTGAAACAGG TTGCAGATAA GCTCCCCCGG 
CCCAACCTCC TGCTACTCAA GCACTTGGTC TATGTGCTGC ACCTCATCAG CAAGAACTCT 
GAGGTGAACA GGATGGACTC CAGCAATCTG GCCATCTGCA TTGGACCCAA CATGCTCACC 
CTGGAGAATG ACCAGAGCCT GTCATTTGAA GCCCAGAAGG ACCTGAACAA CAAGGTGAAG 
ACACTGGTGG AATTCCTCAT TGATAACTGC TTTGAAATAT TTGGGGAGAA CATTCCAGTG 
CATTCCAGTA TCACTTCTGA TGACTCCCTG GAGCACACTG ACAGTTCAGA TGTGTCGACC 
CTGCAGAATG ACTCAGCCTA CGACAGCAAC GACCCTGATG TGGAATCCAA CAGCAGCAGT 
GGCATCAGCT CTCCCAGCAG GCAGCCCCAG GTGCCCATGG CCACAGCTGC TGGCTTGGAT 
AGCGCGGGCC CACAGGATGC CCGAGAGGTC AGCCCAGAGC CCATTGTGAG CACCGTGGCC 
AGGCTGAAAA GCTCCCTCGC ACAGCCCGAT AGGAGATACT CAGAGCCCAG CATGCCATCC 
TCCCAGGAGT GCCTCGAGAG CCGGGTGACA AACCAAACAC TAACAAAGAG TGAAGGGGAC 
TTCCCCGTGC CCCGGGTAGG CTCTCGTTTG GAAAGTGAGG AGGCTGAAGA CCCATTTCCA 
GAGGAGGTCT TCCCTGCAGT GCAAGGCAAA ACCAAGAGGC CGGTGGACCT GAAGATCAAG 
AACTTGGCCC CGGGTTCGGT GCTCCCGCGG GCACTGGTTC TCAAAGCCTT CTCCAGCAGC 
TCGCTGGACG CGTCCTCTGA CAGCTCGCCC GTGGCTTCTC CTTCCAGTCC CAAAAGAAAT 
TTCTTCAGCA GACATCAGTC TTTCACCACA AAGACAGAGA AAGGCAAGCC CAGCCGAGAA 
ATTAAAAAGC ACTCCATGTC TTTCACCTTT GCCCCTCACA AAAAAGTGCT GACCAAAAAC 
CTCAGCGCGG GCTCTGGGAA ATCGCAAGAC TTTACCAGGG ACCACGTCCC GAGGGGTGTC 
AGAAAGGAAA GCCAGCTTGC CGGCCGAATC GTGCAGGAAA ATGGGTGTGA AACCCACAAC 
CAAACAGCCC GCGGCTTCTG CCTGAGACCC CACGCCCTCT CGGTGGATGA TGTGTTCCAG 
GGAGCTGACT GGGAGAGGCC TGGAAGCCCA CCCTCTTATG AAGAGGCCAT GCAGGGCCCG 
GCAGCCAGAC TAGTGGCCTC CGAGAGCCAG ACCGTGGGGA GCATGACGGT GGGGAGCATG 
AGGGCGAGGA TGCTGGAGGC GCACTGCCTC CTACCCCCTC TTCCACCTGC TCACCACGTA 
GAGGACTCAA GACACAGGGG CAGCAAAGAG CCACTCCCTG GCCACGGACT CTCTCCCCTG 
CCTGAGCGAT GGAAACAGAG CAGAACTGTC CATGCTTCTG GGGACTCTCT GGGGCACGTG 
TCTGGCCCAG GGAGACCTGA GCTCCTCCCG CTGAGGACCG TCTCCGAGTC CGTGCAGAGG 
AATAAGCGGG ACTGTCTCGT GCGACGATGT AGCCAGCCGG TCTTTGAGGC TGACCAATTC 
CAATATGCCA AAGAATCGTA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAGAP ( NM_054114.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_473455.2 Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 2, mRNA.

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CloneID	OHu21713
Clone ID Related Accession (Same CDS sequence)	NM_054114.4 , NM_054114.5
Accession Version	NM_054114.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	T-cell activation Rho GTPase-activating protein isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF385429.1 and AL035530.11. This sequence is a reference standard in the RefSeqGene project. On Jul 18, 2013 this sequence version replaced NM_054114.3. Transcript Variant: This variant (2) encodes the longest isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF385429.1, SRR1803616.259414.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCTGA GAAGCAGCCA CAATGCTTCA AAAACACTAA ACGCCAATAA TATGGAGACA 
CTAATCGAAT GTCAATCAGA GGGTGATATC AAGGAACATC CCCTGTTGGC ATCATGTGAG 
AGTGAAGACA GTATTTGCCA GCTCATTGAA GTTAAGAAGA GAAAGAAGGT GCTGTCCTGG 
CCCTTTCTCA TGAGAAGGCT CTCCCCTGCA TCAGATTTTT CTGGGGCTTT GGAGACAGAC 
TTGAAAGCAT CGCTATTTGA TCAGCCCTTG TCAATTATCT GCGGTGACAG TGACACACTC 
CCCAGACCCA TCCAGGACAT TCTCACTATT CTATGCCTTA AAGGCCCTTC AACGGAAGGG 
ATATTCAGGA GAGCAGCCAA CGAGAAAGCC CGTAAGGAGC TGAAGGAGGA GCTCAACTCT 
GGGGATGCGG TGGATCTGGA GAGGCTCCCC GTGCACCTCC TCGCTGTGGT CTTTAAGGAC 
TTCCTCAGAA GTATCCCCCG GAAGCTACTT TCAAGCGACC TCTTTGAGGA GTGGATGGGT 
GCTCTGGAGA TGCAGGACGA GGAGGACAGA ATCGAGGCCC TGAAACAGGT TGCAGATAAG 
CTCCCCCGGC CCAACCTCCT GCTACTCAAG CACTTGGTCT ATGTGCTGCA CCTCATCAGC 
AAGAACTCTG AGGTGAACAG GATGGACTCC AGCAATCTGG CCATCTGCAT TGGACCCAAC 
ATGCTCACCC TGGAGAATGA CCAGAGCCTG TCATTTGAAG CCCAGAAGGA CCTGAACAAC 
AAGGTGAAGA CACTGGTGGA ATTCCTCATT GATAACTGCT TTGAAATATT TGGGGAGAAC 
ATTCCAGTGC ATTCCAGTAT CACTTCTGAT GACTCCCTGG AGCACACTGA CAGTTCAGAT 
GTGTCGACCC TGCAGAATGA CTCAGCCTAC GACAGCAACG ACCCTGATGT GGAATCCAAC 
AGCAGCAGTG GCATCAGCTC TCCCAGCAGG CAGCCCCAGG TGCCCATGGC CACAGCTGCT 
GGCTTGGATA GCGCGGGCCC ACAGGATGCC CGAGAGGTCA GCCCAGAGCC CATTGTGAGC 
ACCGTGGCCA GGCTGAAAAG CTCCCTCGCA CAGCCCGATA GGAGATACTC AGAGCCCAGC 
ATGCCATCCT CCCAGGAGTG CCTCGAGAGC CGGGTGACAA ACCAAACACT AACAAAGAGT 
GAAGGGGACT TCCCCGTGCC CCGGGTAGGC TCTCGTTTGG AAAGTGAGGA GGCTGAAGAC 
CCATTTCCAG AGGAGGTCTT CCCTGCAGTG CAAGGCAAAA CCAAGAGGCC GGTGGACCTG 
AAGATCAAGA ACTTGGCCCC GGGTTCGGTG CTCCCGCGGG CACTGGTTCT CAAAGCCTTC 
TCCAGCAGCT CGCTGGACGC GTCCTCTGAC AGCTCGCCCG TGGCTTCTCC TTCCAGTCCC 
AAAAGAAATT TCTTCAGCAG ACATCAGTCT TTCACCACAA AGACAGAGAA AGGCAAGCCC 
AGCCGAGAAA TTAAAAAGCA CTCCATGTCT TTCACCTTTG CCCCTCACAA AAAAGTGCTG 
ACCAAAAACC TCAGCGCGGG CTCTGGGAAA TCGCAAGACT TTACCAGGGA CCACGTCCCG 
AGGGGTGTCA GAAAGGAAAG CCAGCTTGCC GGCCGAATCG TGCAGGAAAA TGGGTGTGAA 
ACCCACAACC AAACAGCCCG CGGCTTCTGC CTGAGACCCC ACGCCCTCTC GGTGGATGAT 
GTGTTCCAGG GAGCTGACTG GGAGAGGCCT GGAAGCCCAC CCTCTTATGA AGAGGCCATG 
CAGGGCCCGG CAGCCAGACT AGTGGCCTCC GAGAGCCAGA CCGTGGGGAG CATGACGGTG 
GGGAGCATGA GGGCGAGGAT GCTGGAGGCG CACTGCCTCC TACCCCCTCT TCCACCTGCT 
CACCACGTAG AGGACTCAAG ACACAGGGGC AGCAAAGAGC CACTCCCTGG CCACGGACTC 
TCTCCCCTGC CTGAGCGATG GAAACAGAGC AGAACTGTCC ATGCTTCTGG GGACTCTCTG 
GGGCACGTGT CTGGCCCAGG GAGACCTGAG CTCCTCCCGC TGAGGACCGT CTCCGAGTCC 
GTGCAGAGGA ATAAGCGGGA CTGTCTCGTG CGACGATGTA GCCAGCCGGT CTTTGAGGCT 
GACCAATTCC AATATGCCAA AGAATCGTAT ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_473455.2
CDS	333..2528
Translation	MKLRSSHNASKTLNANNMETLIECQSEGDIKEHPLLASCESEDSICQLIEVKKRKKVLSWPFLMRRLSPASDFSGALETDLKASLFDQPLSIICGDSDTLPRPIQDILTILCLKGPSTEGIFRRAANEKARKELKEELNSGDAVDLERLPVHLLAVVFKDFLRSIPRKLLSSDLFEEWMGALEMQDEEDRIEALKQVADKLPRPNLLLLKHLVYVLHLISKNSEVNRMDSSNLAICIGPNMLTLENDQSLSFEAQKDLNNKVKTLVEFLIDNCFEIFGENIPVHSSITSDDSLEHTDSSDVSTLQNDSAYDSNDPDVESNSSSGISSPSRQPQVPMATAAGLDSAGPQDAREVSPEPIVSTVARLKSSLAQPDRRYSEPSMPSSQECLESRVTNQTLTKSEGDFPVPRVGSRLESEEAEDPFPEEVFPAVQGKTKRPVDLKIKNLAPGSVLPRALVLKAFSSSSLDASSDSSPVASPSSPKRNFFSRHQSFTTKTEKGKPSREIKKHSMSFTFAPHKKVLTKNLSAGSGKSQDFTRDHVPRGVRKESQLAGRIVQENGCETHNQTARGFCLRPHALSVDDVFQGADWERPGSPPSYEEAMQGPAARLVASESQTVGSMTVGSMRARMLEAHCLLPPLPPAHHVEDSRHRGSKEPLPGHGLSPLPERWKQSRTVHASGDSLGHVSGPGRPELLPLRTVSESVQRNKRDCLVRRCSQPVFEADQFQYAKESYI

Target ORF information:

RefSeq Version	NM_054114.4
Organism	Homo sapiens(human)
Definition	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_054114.4

ORF Insert Sequence:

ATGAAGCTGA GAAGCAGCCA CAATGCTTCA AAAACACTAA ACGCCAATAA TATGGAGACA 
CTAATCGAAT GTCAATCAGA GGGTGATATC AAGGAACATC CCCTGTTGGC ATCATGTGAG 
AGTGAAGACA GTATTTGCCA GCTCATTGAA GTTAAGAAGA GAAAGAAGGT GCTGTCCTGG 
CCCTTTCTCA TGAGAAGGCT CTCCCCTGCA TCAGATTTTT CTGGGGCTTT GGAGACAGAC 
TTGAAAGCAT CGCTATTTGA TCAGCCCTTG TCAATTATCT GCGGTGACAG TGACACACTC 
CCCAGACCCA TCCAGGACAT TCTCACTATT CTATGCCTTA AAGGCCCTTC AACGGAAGGG 
ATATTCAGGA GAGCAGCCAA CGAGAAAGCC CGTAAGGAGC TGAAGGAGGA GCTCAACTCT 
GGGGATGCGG TGGATCTGGA GAGGCTCCCC GTGCACCTCC TCGCTGTGGT CTTTAAGGAC 
TTCCTCAGAA GTATCCCCCG GAAGCTACTT TCAAGCGACC TCTTTGAGGA GTGGATGGGT 
GCTCTGGAGA TGCAGGACGA GGAGGACAGA ATCGAGGCCC TGAAACAGGT TGCAGATAAG 
CTCCCCCGGC CCAACCTCCT GCTACTCAAG CACTTGGTCT ATGTGCTGCA CCTCATCAGC 
AAGAACTCTG AGGTGAACAG GATGGACTCC AGCAATCTGG CCATCTGCAT TGGACCCAAC 
ATGCTCACCC TGGAGAATGA CCAGAGCCTG TCATTTGAAG CCCAGAAGGA CCTGAACAAC 
AAGGTGAAGA CACTGGTGGA ATTCCTCATT GATAACTGCT TTGAAATATT TGGGGAGAAC 
ATTCCAGTGC ATTCCAGTAT CACTTCTGAT GACTCCCTGG AGCACACTGA CAGTTCAGAT 
GTGTCGACCC TGCAGAATGA CTCAGCCTAC GACAGCAACG ACCCTGATGT GGAATCCAAC 
AGCAGCAGTG GCATCAGCTC TCCCAGCAGG CAGCCCCAGG TGCCCATGGC CACAGCTGCT 
GGCTTGGATA GCGCGGGCCC ACAGGATGCC CGAGAGGTCA GCCCAGAGCC CATTGTGAGC 
ACCGTGGCCA GGCTGAAAAG CTCCCTCGCA CAGCCCGATA GGAGATACTC AGAGCCCAGC 
ATGCCATCCT CCCAGGAGTG CCTCGAGAGC CGGGTGACAA ACCAAACACT AACAAAGAGT 
GAAGGGGACT TCCCCGTGCC CCGGGTAGGC TCTCGTTTGG AAAGTGAGGA GGCTGAAGAC 
CCATTTCCAG AGGAGGTCTT CCCTGCAGTG CAAGGCAAAA CCAAGAGGCC GGTGGACCTG 
AAGATCAAGA ACTTGGCCCC GGGTTCGGTG CTCCCGCGGG CACTGGTTCT CAAAGCCTTC 
TCCAGCAGCT CGCTGGACGC GTCCTCTGAC AGCTCGCCCG TGGCTTCTCC TTCCAGTCCC 
AAAAGAAATT TCTTCAGCAG ACATCAGTCT TTCACCACAA AGACAGAGAA AGGCAAGCCC 
AGCCGAGAAA TTAAAAAGCA CTCCATGTCT TTCACCTTTG CCCCTCACAA AAAAGTGCTG 
ACCAAAAACC TCAGCGCGGG CTCTGGGAAA TCGCAAGACT TTACCAGGGA CCACGTCCCG 
AGGGGTGTCA GAAAGGAAAG CCAGCTTGCC GGCCGAATCG TGCAGGAAAA TGGGTGTGAA 
ACCCACAACC AAACAGCCCG CGGCTTCTGC CTGAGACCCC ACGCCCTCTC GGTGGATGAT 
GTGTTCCAGG GAGCTGACTG GGAGAGGCCT GGAAGCCCAC CCTCTTATGA AGAGGCCATG 
CAGGGCCCGG CAGCCAGACT AGTGGCCTCC GAGAGCCAGA CCGTGGGGAG CATGACGGTG 
GGGAGCATGA GGGCGAGGAT GCTGGAGGCG CACTGCCTCC TACCCCCTCT TCCACCTGCT 
CACCACGTAG AGGACTCAAG ACACAGGGGC AGCAAAGAGC CACTCCCTGG CCACGGACTC 
TCTCCCCTGC CTGAGCGATG GAAACAGAGC AGAACTGTCC ATGCTTCTGG GGACTCTCTG 
GGGCACGTGT CTGGCCCAGG GAGACCTGAG CTCCTCCCGC TGAGGACCGT CTCCGAGTCC 
GTGCAGAGGA ATAAGCGGGA CTGTCTCGTG CGACGATGTA GCCAGCCGGT CTTTGAGGCT 
GACCAATTCC AATATGCCAA AGAATCGTAT ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAGAP ( NM_054114.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_473455.2 Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21713
Clone ID Related Accession (Same CDS sequence)	NM_054114.4 , NM_054114.5
Accession Version	NM_054114.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	T-cell activation Rho GTPase-activating protein isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF385429.1 and AL035530.11. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_054114.4. Transcript Variant: This variant (2) encodes the longest isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.259414.1, AK097090.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGCTGA GAAGCAGCCA CAATGCTTCA AAAACACTAA ACGCCAATAA TATGGAGACA 
CTAATCGAAT GTCAATCAGA GGGTGATATC AAGGAACATC CCCTGTTGGC ATCATGTGAG 
AGTGAAGACA GTATTTGCCA GCTCATTGAA GTTAAGAAGA GAAAGAAGGT GCTGTCCTGG 
CCCTTTCTCA TGAGAAGGCT CTCCCCTGCA TCAGATTTTT CTGGGGCTTT GGAGACAGAC 
TTGAAAGCAT CGCTATTTGA TCAGCCCTTG TCAATTATCT GCGGTGACAG TGACACACTC 
CCCAGACCCA TCCAGGACAT TCTCACTATT CTATGCCTTA AAGGCCCTTC AACGGAAGGG 
ATATTCAGGA GAGCAGCCAA CGAGAAAGCC CGTAAGGAGC TGAAGGAGGA GCTCAACTCT 
GGGGATGCGG TGGATCTGGA GAGGCTCCCC GTGCACCTCC TCGCTGTGGT CTTTAAGGAC 
TTCCTCAGAA GTATCCCCCG GAAGCTACTT TCAAGCGACC TCTTTGAGGA GTGGATGGGT 
GCTCTGGAGA TGCAGGACGA GGAGGACAGA ATCGAGGCCC TGAAACAGGT TGCAGATAAG 
CTCCCCCGGC CCAACCTCCT GCTACTCAAG CACTTGGTCT ATGTGCTGCA CCTCATCAGC 
AAGAACTCTG AGGTGAACAG GATGGACTCC AGCAATCTGG CCATCTGCAT TGGACCCAAC 
ATGCTCACCC TGGAGAATGA CCAGAGCCTG TCATTTGAAG CCCAGAAGGA CCTGAACAAC 
AAGGTGAAGA CACTGGTGGA ATTCCTCATT GATAACTGCT TTGAAATATT TGGGGAGAAC 
ATTCCAGTGC ATTCCAGTAT CACTTCTGAT GACTCCCTGG AGCACACTGA CAGTTCAGAT 
GTGTCGACCC TGCAGAATGA CTCAGCCTAC GACAGCAACG ACCCTGATGT GGAATCCAAC 
AGCAGCAGTG GCATCAGCTC TCCCAGCAGG CAGCCCCAGG TGCCCATGGC CACAGCTGCT 
GGCTTGGATA GCGCGGGCCC ACAGGATGCC CGAGAGGTCA GCCCAGAGCC CATTGTGAGC 
ACCGTGGCCA GGCTGAAAAG CTCCCTCGCA CAGCCCGATA GGAGATACTC AGAGCCCAGC 
ATGCCATCCT CCCAGGAGTG CCTCGAGAGC CGGGTGACAA ACCAAACACT AACAAAGAGT 
GAAGGGGACT TCCCCGTGCC CCGGGTAGGC TCTCGTTTGG AAAGTGAGGA GGCTGAAGAC 
CCATTTCCAG AGGAGGTCTT CCCTGCAGTG CAAGGCAAAA CCAAGAGGCC GGTGGACCTG 
AAGATCAAGA ACTTGGCCCC GGGTTCGGTG CTCCCGCGGG CACTGGTTCT CAAAGCCTTC 
TCCAGCAGCT CGCTGGACGC GTCCTCTGAC AGCTCGCCCG TGGCTTCTCC TTCCAGTCCC 
AAAAGAAATT TCTTCAGCAG ACATCAGTCT TTCACCACAA AGACAGAGAA AGGCAAGCCC 
AGCCGAGAAA TTAAAAAGCA CTCCATGTCT TTCACCTTTG CCCCTCACAA AAAAGTGCTG 
ACCAAAAACC TCAGCGCGGG CTCTGGGAAA TCGCAAGACT TTACCAGGGA CCACGTCCCG 
AGGGGTGTCA GAAAGGAAAG CCAGCTTGCC GGCCGAATCG TGCAGGAAAA TGGGTGTGAA 
ACCCACAACC AAACAGCCCG CGGCTTCTGC CTGAGACCCC ACGCCCTCTC GGTGGATGAT 
GTGTTCCAGG GAGCTGACTG GGAGAGGCCT GGAAGCCCAC CCTCTTATGA AGAGGCCATG 
CAGGGCCCGG CAGCCAGACT AGTGGCCTCC GAGAGCCAGA CCGTGGGGAG CATGACGGTG 
GGGAGCATGA GGGCGAGGAT GCTGGAGGCG CACTGCCTCC TACCCCCTCT TCCACCTGCT 
CACCACGTAG AGGACTCAAG ACACAGGGGC AGCAAAGAGC CACTCCCTGG CCACGGACTC 
TCTCCCCTGC CTGAGCGATG GAAACAGAGC AGAACTGTCC ATGCTTCTGG GGACTCTCTG 
GGGCACGTGT CTGGCCCAGG GAGACCTGAG CTCCTCCCGC TGAGGACCGT CTCCGAGTCC 
GTGCAGAGGA ATAAGCGGGA CTGTCTCGTG CGACGATGTA GCCAGCCGGT CTTTGAGGCT 
GACCAATTCC AATATGCCAA AGAATCGTAT ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_473455.2
CDS	172..2367
Translation	MKLRSSHNASKTLNANNMETLIECQSEGDIKEHPLLASCESEDSICQLIEVKKRKKVLSWPFLMRRLSPASDFSGALETDLKASLFDQPLSIICGDSDTLPRPIQDILTILCLKGPSTEGIFRRAANEKARKELKEELNSGDAVDLERLPVHLLAVVFKDFLRSIPRKLLSSDLFEEWMGALEMQDEEDRIEALKQVADKLPRPNLLLLKHLVYVLHLISKNSEVNRMDSSNLAICIGPNMLTLENDQSLSFEAQKDLNNKVKTLVEFLIDNCFEIFGENIPVHSSITSDDSLEHTDSSDVSTLQNDSAYDSNDPDVESNSSSGISSPSRQPQVPMATAAGLDSAGPQDAREVSPEPIVSTVARLKSSLAQPDRRYSEPSMPSSQECLESRVTNQTLTKSEGDFPVPRVGSRLESEEAEDPFPEEVFPAVQGKTKRPVDLKIKNLAPGSVLPRALVLKAFSSSSLDASSDSSPVASPSSPKRNFFSRHQSFTTKTEKGKPSREIKKHSMSFTFAPHKKVLTKNLSAGSGKSQDFTRDHVPRGVRKESQLAGRIVQENGCETHNQTARGFCLRPHALSVDDVFQGADWERPGSPPSYEEAMQGPAARLVASESQTVGSMTVGSMRARMLEAHCLLPPLPPAHHVEDSRHRGSKEPLPGHGLSPLPERWKQSRTVHASGDSLGHVSGPGRPELLPLRTVSESVQRNKRDCLVRRCSQPVFEADQFQYAKESYI

Target ORF information:

RefSeq Version	NM_054114.5
Organism	Homo sapiens(human)
Definition	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_054114.5

ORF Insert Sequence:

ATGAAGCTGA GAAGCAGCCA CAATGCTTCA AAAACACTAA ACGCCAATAA TATGGAGACA 
CTAATCGAAT GTCAATCAGA GGGTGATATC AAGGAACATC CCCTGTTGGC ATCATGTGAG 
AGTGAAGACA GTATTTGCCA GCTCATTGAA GTTAAGAAGA GAAAGAAGGT GCTGTCCTGG 
CCCTTTCTCA TGAGAAGGCT CTCCCCTGCA TCAGATTTTT CTGGGGCTTT GGAGACAGAC 
TTGAAAGCAT CGCTATTTGA TCAGCCCTTG TCAATTATCT GCGGTGACAG TGACACACTC 
CCCAGACCCA TCCAGGACAT TCTCACTATT CTATGCCTTA AAGGCCCTTC AACGGAAGGG 
ATATTCAGGA GAGCAGCCAA CGAGAAAGCC CGTAAGGAGC TGAAGGAGGA GCTCAACTCT 
GGGGATGCGG TGGATCTGGA GAGGCTCCCC GTGCACCTCC TCGCTGTGGT CTTTAAGGAC 
TTCCTCAGAA GTATCCCCCG GAAGCTACTT TCAAGCGACC TCTTTGAGGA GTGGATGGGT 
GCTCTGGAGA TGCAGGACGA GGAGGACAGA ATCGAGGCCC TGAAACAGGT TGCAGATAAG 
CTCCCCCGGC CCAACCTCCT GCTACTCAAG CACTTGGTCT ATGTGCTGCA CCTCATCAGC 
AAGAACTCTG AGGTGAACAG GATGGACTCC AGCAATCTGG CCATCTGCAT TGGACCCAAC 
ATGCTCACCC TGGAGAATGA CCAGAGCCTG TCATTTGAAG CCCAGAAGGA CCTGAACAAC 
AAGGTGAAGA CACTGGTGGA ATTCCTCATT GATAACTGCT TTGAAATATT TGGGGAGAAC 
ATTCCAGTGC ATTCCAGTAT CACTTCTGAT GACTCCCTGG AGCACACTGA CAGTTCAGAT 
GTGTCGACCC TGCAGAATGA CTCAGCCTAC GACAGCAACG ACCCTGATGT GGAATCCAAC 
AGCAGCAGTG GCATCAGCTC TCCCAGCAGG CAGCCCCAGG TGCCCATGGC CACAGCTGCT 
GGCTTGGATA GCGCGGGCCC ACAGGATGCC CGAGAGGTCA GCCCAGAGCC CATTGTGAGC 
ACCGTGGCCA GGCTGAAAAG CTCCCTCGCA CAGCCCGATA GGAGATACTC AGAGCCCAGC 
ATGCCATCCT CCCAGGAGTG CCTCGAGAGC CGGGTGACAA ACCAAACACT AACAAAGAGT 
GAAGGGGACT TCCCCGTGCC CCGGGTAGGC TCTCGTTTGG AAAGTGAGGA GGCTGAAGAC 
CCATTTCCAG AGGAGGTCTT CCCTGCAGTG CAAGGCAAAA CCAAGAGGCC GGTGGACCTG 
AAGATCAAGA ACTTGGCCCC GGGTTCGGTG CTCCCGCGGG CACTGGTTCT CAAAGCCTTC 
TCCAGCAGCT CGCTGGACGC GTCCTCTGAC AGCTCGCCCG TGGCTTCTCC TTCCAGTCCC 
AAAAGAAATT TCTTCAGCAG ACATCAGTCT TTCACCACAA AGACAGAGAA AGGCAAGCCC 
AGCCGAGAAA TTAAAAAGCA CTCCATGTCT TTCACCTTTG CCCCTCACAA AAAAGTGCTG 
ACCAAAAACC TCAGCGCGGG CTCTGGGAAA TCGCAAGACT TTACCAGGGA CCACGTCCCG 
AGGGGTGTCA GAAAGGAAAG CCAGCTTGCC GGCCGAATCG TGCAGGAAAA TGGGTGTGAA 
ACCCACAACC AAACAGCCCG CGGCTTCTGC CTGAGACCCC ACGCCCTCTC GGTGGATGAT 
GTGTTCCAGG GAGCTGACTG GGAGAGGCCT GGAAGCCCAC CCTCTTATGA AGAGGCCATG 
CAGGGCCCGG CAGCCAGACT AGTGGCCTCC GAGAGCCAGA CCGTGGGGAG CATGACGGTG 
GGGAGCATGA GGGCGAGGAT GCTGGAGGCG CACTGCCTCC TACCCCCTCT TCCACCTGCT 
CACCACGTAG AGGACTCAAG ACACAGGGGC AGCAAAGAGC CACTCCCTGG CCACGGACTC 
TCTCCCCTGC CTGAGCGATG GAAACAGAGC AGAACTGTCC ATGCTTCTGG GGACTCTCTG 
GGGCACGTGT CTGGCCCAGG GAGACCTGAG CTCCTCCCGC TGAGGACCGT CTCCGAGTCC 
GTGCAGAGGA ATAAGCGGGA CTGTCTCGTG CGACGATGTA GCCAGCCGGT CTTTGAGGCT 
GACCAATTCC AATATGCCAA AGAATCGTAT ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAGAP ( NM_138810.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_620165.1 Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 3, mRNA.

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CloneID	OHu20790
Clone ID Related Accession (Same CDS sequence)	NM_138810.3 , NM_138810.4
Accession Version	NM_138810.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	T-cell activation Rho GTPase-activating protein isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF385429.1, BC015859.1 and AL035530.11. On Jul 18, 2013 this sequence version replaced NM_138810.2. Transcript Variant: This variant (3) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 2. The encoded isoform (c) has a shorter and distinct C-terminus, compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015859.1, SRR1163657.68875.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCTGA GAAGCAGCCA CAATGCTTCA AAAACACTAA ACGCCAATAA TATGGAGACA 
CTAATCGAAT GTCAATCAGA GGGTGATATC AAGGAACATC CCCTGTTGGC ATCATGTGAG 
AGTGAAGACA GTATTTGCCA GCTCATTGAA GTTAAGAAGA GAAAGAAGGT GCTGTCCTGG 
CCCTTTCTCA TGAGAAGGCT CTCCCCTGCA TCAGATTTTT CTGGGGCTTT GGAGACAGAC 
TTGAAAGCAT CGCTATTTGA TCAGCCCTTG TCAATTATCT GCGGTGACAG TGACACACTC 
CCCAGACCCA TCCAGGACAT TCTCACTATT CTATGCCTTA AAGGCCCTTC AACGGAAGGG 
ATATTCAGGA GAGCAGCCAA CGAGAAAGCC CGTAAGGAGC TGAAGGAGGA GCTCAACTCT 
GGGGATGCGG TGGATCTGGA GAGGCTCCCC GTGCACCTCC TCGCTGTGGT CTTTAAGGAC 
TTCCTCAGAA GTATCCCCCG GAAGCTACTT TCAAGCGACC TCTTTGAGGA GTGGATGGGT 
GCTCTGGAGA TGCAGGACGA GGAGGACAGA ATCGAGGCCC TGAAACAGGT TGCAGATAAG 
CTCCCCCGGC CCAACCTCCT GCTACTCAAG CACTTGGTCT ATGTGCTGCA CCTCATCAGC 
AAGAACTCTG AGGTGAACAG GATGGACTCC AGCAATCTGG CCATCTGCAT TGGACCCAAC 
ATGCTCACCC TGGAGAATGA CCAGAGCCTG TCATTTGAAG CCCAGAAGGA CCTGAACAAC 
AAGGTTTGTT CTGCTTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620165.1
CDS	333..1133
Translation	MKLRSSHNASKTLNANNMETLIECQSEGDIKEHPLLASCESEDSICQLIEVKKRKKVLSWPFLMRRLSPASDFSGALETDLKASLFDQPLSIICGDSDTLPRPIQDILTILCLKGPSTEGIFRRAANEKARKELKEELNSGDAVDLERLPVHLLAVVFKDFLRSIPRKLLSSDLFEEWMGALEMQDEEDRIEALKQVADKLPRPNLLLLKHLVYVLHLISKNSEVNRMDSSNLAICIGPNMLTLENDQSLSFEAQKDLNNKVCSAY

Target ORF information:

RefSeq Version	NM_138810.3
Organism	Homo sapiens(human)
Definition	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138810.3

ORF Insert Sequence:

ATGAAGCTGA GAAGCAGCCA CAATGCTTCA AAAACACTAA ACGCCAATAA TATGGAGACA 
CTAATCGAAT GTCAATCAGA GGGTGATATC AAGGAACATC CCCTGTTGGC ATCATGTGAG 
AGTGAAGACA GTATTTGCCA GCTCATTGAA GTTAAGAAGA GAAAGAAGGT GCTGTCCTGG 
CCCTTTCTCA TGAGAAGGCT CTCCCCTGCA TCAGATTTTT CTGGGGCTTT GGAGACAGAC 
TTGAAAGCAT CGCTATTTGA TCAGCCCTTG TCAATTATCT GCGGTGACAG TGACACACTC 
CCCAGACCCA TCCAGGACAT TCTCACTATT CTATGCCTTA AAGGCCCTTC AACGGAAGGG 
ATATTCAGGA GAGCAGCCAA CGAGAAAGCC CGTAAGGAGC TGAAGGAGGA GCTCAACTCT 
GGGGATGCGG TGGATCTGGA GAGGCTCCCC GTGCACCTCC TCGCTGTGGT CTTTAAGGAC 
TTCCTCAGAA GTATCCCCCG GAAGCTACTT TCAAGCGACC TCTTTGAGGA GTGGATGGGT 
GCTCTGGAGA TGCAGGACGA GGAGGACAGA ATCGAGGCCC TGAAACAGGT TGCAGATAAG 
CTCCCCCGGC CCAACCTCCT GCTACTCAAG CACTTGGTCT ATGTGCTGCA CCTCATCAGC 
AAGAACTCTG AGGTGAACAG GATGGACTCC AGCAATCTGG CCATCTGCAT TGGACCCAAC 
ATGCTCACCC TGGAGAATGA CCAGAGCCTG TCATTTGAAG CCCAGAAGGA CCTGAACAAC 
AAGGTTTGTT CTGCTTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAGAP ( NM_138810.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_620165.1 Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu20790
Clone ID Related Accession (Same CDS sequence)	NM_138810.3 , NM_138810.4
Accession Version	NM_138810.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-08
Organism	Homo sapiens(human)
Product	T-cell activation Rho GTPase-activating protein isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF385429.1, BC015859.1 and AL035530.11. On Jun 2, 2019 this sequence version replaced NM_138810.3. Transcript Variant: This variant (3) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 2. The encoded isoform (c) has a shorter and distinct C-terminus, compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.68875.1, BC015859.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCTGA GAAGCAGCCA CAATGCTTCA AAAACACTAA ACGCCAATAA TATGGAGACA 
CTAATCGAAT GTCAATCAGA GGGTGATATC AAGGAACATC CCCTGTTGGC ATCATGTGAG 
AGTGAAGACA GTATTTGCCA GCTCATTGAA GTTAAGAAGA GAAAGAAGGT GCTGTCCTGG 
CCCTTTCTCA TGAGAAGGCT CTCCCCTGCA TCAGATTTTT CTGGGGCTTT GGAGACAGAC 
TTGAAAGCAT CGCTATTTGA TCAGCCCTTG TCAATTATCT GCGGTGACAG TGACACACTC 
CCCAGACCCA TCCAGGACAT TCTCACTATT CTATGCCTTA AAGGCCCTTC AACGGAAGGG 
ATATTCAGGA GAGCAGCCAA CGAGAAAGCC CGTAAGGAGC TGAAGGAGGA GCTCAACTCT 
GGGGATGCGG TGGATCTGGA GAGGCTCCCC GTGCACCTCC TCGCTGTGGT CTTTAAGGAC 
TTCCTCAGAA GTATCCCCCG GAAGCTACTT TCAAGCGACC TCTTTGAGGA GTGGATGGGT 
GCTCTGGAGA TGCAGGACGA GGAGGACAGA ATCGAGGCCC TGAAACAGGT TGCAGATAAG 
CTCCCCCGGC CCAACCTCCT GCTACTCAAG CACTTGGTCT ATGTGCTGCA CCTCATCAGC 
AAGAACTCTG AGGTGAACAG GATGGACTCC AGCAATCTGG CCATCTGCAT TGGACCCAAC 
ATGCTCACCC TGGAGAATGA CCAGAGCCTG TCATTTGAAG CCCAGAAGGA CCTGAACAAC 
AAGGTTTGTT CTGCTTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620165.1
CDS	172..972
Translation	MKLRSSHNASKTLNANNMETLIECQSEGDIKEHPLLASCESEDSICQLIEVKKRKKVLSWPFLMRRLSPASDFSGALETDLKASLFDQPLSIICGDSDTLPRPIQDILTILCLKGPSTEGIFRRAANEKARKELKEELNSGDAVDLERLPVHLLAVVFKDFLRSIPRKLLSSDLFEEWMGALEMQDEEDRIEALKQVADKLPRPNLLLLKHLVYVLHLISKNSEVNRMDSSNLAICIGPNMLTLENDQSLSFEAQKDLNNKVCSAY

Target ORF information:

RefSeq Version	NM_138810.4
Organism	Homo sapiens(human)
Definition	Homo sapiens T cell activation RhoGTPase activating protein (TAGAP), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138810.4

ORF Insert Sequence:

ATGAAGCTGA GAAGCAGCCA CAATGCTTCA AAAACACTAA ACGCCAATAA TATGGAGACA 
CTAATCGAAT GTCAATCAGA GGGTGATATC AAGGAACATC CCCTGTTGGC ATCATGTGAG 
AGTGAAGACA GTATTTGCCA GCTCATTGAA GTTAAGAAGA GAAAGAAGGT GCTGTCCTGG 
CCCTTTCTCA TGAGAAGGCT CTCCCCTGCA TCAGATTTTT CTGGGGCTTT GGAGACAGAC 
TTGAAAGCAT CGCTATTTGA TCAGCCCTTG TCAATTATCT GCGGTGACAG TGACACACTC 
CCCAGACCCA TCCAGGACAT TCTCACTATT CTATGCCTTA AAGGCCCTTC AACGGAAGGG 
ATATTCAGGA GAGCAGCCAA CGAGAAAGCC CGTAAGGAGC TGAAGGAGGA GCTCAACTCT 
GGGGATGCGG TGGATCTGGA GAGGCTCCCC GTGCACCTCC TCGCTGTGGT CTTTAAGGAC 
TTCCTCAGAA GTATCCCCCG GAAGCTACTT TCAAGCGACC TCTTTGAGGA GTGGATGGGT 
GCTCTGGAGA TGCAGGACGA GGAGGACAGA ATCGAGGCCC TGAAACAGGT TGCAGATAAG 
CTCCCCCGGC CCAACCTCCT GCTACTCAAG CACTTGGTCT ATGTGCTGCA CCTCATCAGC 
AAGAACTCTG AGGTGAACAG GATGGACTCC AGCAATCTGG CCATCTGCAT TGGACCCAAC 
ATGCTCACCC TGGAGAATGA CCAGAGCCTG TCATTTGAAG CCCAGAAGGA CCTGAACAAC 
AAGGTTTGTT CTGCTTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification and in silico analysis of functional SNPs of human TAGAP protein: A comprehensive study.
PloS one13(1)e0188143(2018)
Arshad M,Bhatti A,John P

T cell activation Rho GTPase activating protein (TAGAP) is upregulated in clinical and experimental arthritis.
Cytokine(2017 Oct)
Arshad M,Bhatti A,John P,Jalil F,Borghese F,Kawalkowska JZ,Williams RO,Clanchy FIL

Association of rs1738074 polymorphism of TAGAP gene with susceptibility to multiple sclerosis in the Iranian population.
Neuroscience letters64866-69(2017 May)
Jazaeri A,Vallian S

Association of LPP and TAGAP Polymorphisms with Celiac Disease Risk: A Meta-Analysis.
International journal of environmental research and public health14(2)(2017 02)
Huang SQ,Zhang N,Zhou ZX,Huang CC,Zeng CL,Xiao D,Guo CC,Han YJ,Ye XH,Ye XG,Ou ML,Zhang BH,Liu Y,Zeng EY,Yang G,Jing CX

The multiple sclerosis susceptibility genes TAGAP and IL2RA are regulated by vitamin D in CD4+ T cells.
Genes and immunity17(2)118-27(2016 Mar)
Berge T,Leikfoss IS,Brorson IS,Bos SD,Page CM,Gustavsen MW,Bj?lgerud A,Holm?y T,Celius EG,Damoiseaux J,Smolders J,Harbo HF,Spurkland A

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TAGAP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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