AGAP2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGAP2
Entrez Gene ID	116986
Full Name	ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Synonyms	CENTG1,GGAP2,PIKE
General protein information	Preferred Names ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 Names arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2 GTP-binding and GTPase activating protein 2 centaurin, gamma 1 cnt-g1 phosphatidylinositol 3-kinase enhancer phosphoinositide 3-kinase enhancer
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q14.1
Summary	The protein encoded by this gene belongs to the centaurin gamma-like family. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. It is overexpressed in cancer cells, and promotes cancer cell invasion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011].
Disorder MIM:	605476

mRNA and Protein(s)

mRNA	Protein	Name
NM_014770.4	NP_055585.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform PIKE-A
XM_017018772.1	XP_016874261.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1
XM_005268626.2	XP_005268683.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X3
XM_017018772.2	XP_016874261.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1
XM_005268625.3	XP_005268682.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X2
NM_001122772.2	NP_001116244.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform PIKE-L
NM_014770.3	NP_055585.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform PIKE-A

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100496997	XP_002933734.2
Bos taurus (cattle)	AGAP2	NP_001178968.1
Rattus norvegicus (Norway rat)	Agap2	NP_075415.2
Danio rerio (zebrafish)	agap2	NP_001073487.1
Pan troglodytes (chimpanzee)	AGAP2	XP_509171.3
Mus musculus (house mouse)	Agap2	NP_001028435.1
	AGAP2	XP_001116285.1
Homo sapiens (human)	AGAP2	NP_001116244.1

Gene Ontology
Bibliography

Related articles in PubMed

Co-amplification of phosphoinositide 3-kinase enhancer A and cyclin-dependent kinase 4 triggers glioblastoma progression.
Qi Q, Kang SS, Zhang S, Pham C, Fu H, Brat DJ, Ye K
Oncogene36(32)4562-4572(2017 Aug)

?-Synuclein binds and sequesters PIKE-L into Lewy bodies, triggering dopaminergic cell death via AMPK hyperactivation.
Kang SS, Zhang Z, Liu X, Manfredsson FP, He L, Iuvone PM, Cao X, Sun YE, Jin L, Ye K
Proceedings of the National Academy of Sciences of the United States of America114(5)1183-1188(2017 Jan)

Phosphoinositide-3-Kinase Enhancers, PIKEs: Their Biological Functions and Roles in Cancer.
Jia W, Feng YI, Sanders AJ, Davies EL, Jiang WG
Anticancer research36(3)1103-9(2016 Mar)

Reduced expression of E-cadherin and p120-catenin and elevated expression of PLC-?1 and PIKE are associated with aggressiveness of oral squamous cell carcinoma.
Jiang Y, Liao L, Shrestha C, Ji S, Chen Y, Peng J, Wang L, Liao E, Xie Z
International journal of clinical and experimental pathology8(8)9042-51(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these findings support that PIKE amplification or overexpression coordinately acts with CDK4 to drive glioblastoma tumorigenesis.
Title: Co-amplification of phosphoinositide 3-kinase enhancer A and cyclin-dependent kinase 4 triggers glioblastoma progression.

Studies showed that overexpression or mutation of PIKE has been observed in a variety of tumors, promoting cancer cell growth, transformation and invasion through AKT signaling or other signaling pathways, such as focal adhesion kinase. [review]
Title: Phosphoinositide-3-Kinase Enhancers, PIKEs: Their Biological Functions and Roles in Cancer.

Mutation of Fyn phosphorylation sites on PIKE-A, depletion of Fyn, or pharmacological inhibition of Fyn blunts the association between PIKE-A and AMPK, resulting in loss of its inhibitory effect on AMPK.
Title: Fyn-phosphorylated PIKE-A binds and inhibits AMPK signaling, blocking its tumor suppressive activity.

PIKE reduction rescued PI3K-dependent and -independent neuronal defects in fragile X syndrome.
Title: Increased expression of the PI3K enhancer PIKE mediates deficits in synaptic plasticity and behavior in fragile X syndrome.

expression of PLC-gamma1 and PIKE positively correlated with the tumor differentiation of oral squamous cell carcinoma.
Title: Reduced expression of E-cadherin and p120-catenin and elevated expression of PLC-?1 and PIKE are associated with aggressiveness of oral squamous cell carcinoma.

The following AGAP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGAP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18742	NM_014770.4 Latest version!	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu11929	NM_001122772.2 Latest version!	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$839.30 $1199.00
OHu11929	XM_017018772.2 Latest version!	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$839.30 $1199.00
OHu46299	XM_005268625.3 Latest version!	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu46300	XM_005268626.2 Latest version!	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu11929	XM_017018772.1	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$839.30 $1199.00
OHu18742	NM_014770.3	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGAP2 ( NM_014770.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055585.1 Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18742
Clone ID Related Accession (Same CDS sequence)	NM_014770.4 , NM_014770.3
Accession Version	NM_014770.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform PIKE-A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB165417.1, D79989.1, BI755875.1, BE280072.1, AV740795.1 and AC025165.27. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_014770.3. Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. This results in a shorter isoform (PIKE-A) with a distinct N-terminus compared to isoform PIKE-L. This isoform is reported to be overexpressed in brain tumors and promote cancer cell invasion (PMID:14761976). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D79989.1, AF384128.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGCCC AGAGGCAGTT CGTTGTAGCT GCAGTGAGAG CAGAAGTCAG ACGACATGAG 
GTGGCCAAGC AGGCTCTAAA CCGCCTCAGG AAGCTGGCAG AGAGGGTGGA CGACCCCGAA 
CTCCAGGACA GCATCCAGGC CTCATTGGAC AGCATTCGAG AGGCTGTGAT CAATAGCCAG 
GAATGGACTT TGAGCCGCTC CATTCCTGAA CTGCGCCTGG GTGTGCTGGG CGATGCCAGG 
AGTGGGAAGT CATCGCTCAT CCACCGATTC CTGACTGGCT CATACCAGGT GCTGGAGAAG 
ACAGAGAGTG AGCAGTACAA GAAAGAAATG TTGGTGGATG GACAGACACA TCTGGTGCTA 
ATCCGAGAGG AAGCTGGGGC ACCTGATGCC AAGTTCTCAG GCTGGGCAGA TGCTGTGATC 
TTCGTCTTCA GCCTGGAGGA TGAGAACAGT TTCCAGGCTG TGAGCCGTCT CCATGGGCAG 
CTGAGTTCCC TTCGCGGGGA GGGACGAGGA GGCCTGGCCT TGGCACTGGT GGGGACACAA 
GACAGGATCA GTGCTTCCTC CCCTCGGGTG GTGGGAGATG CTCGTGCCAG AGCTCTGTGC 
GCGGACATGA AACGCTGCAG CTACTATGAG ACTTGTGCAA CCTATGGGCT CAATGTGGAT 
CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG GTGACCTTGC GCAAGCAGCA ACAGCTTCTG 
GCTGCCTGCA AGTCCCTGCC CAGCTCCCCA AGCCACTCAG CTGCATCCAC TCCGGTAGCT 
GGCCAGGCTA GTAACGGGGG CCACACTAGC GACTACTCTT CTTCCCTCCC GTCCTCACCG 
AATGTTGGTC ACCGGGAGCT CCGAGCCGAG GCAGCTGCAG TGGCTGGATT GAGCACCCCA 
GGGTCCCTGC ACCGGGCAGC CAAGCGCAGG ACCAGCCTTT TTGCGAATCG TCGGGGTAGT 
GACTCCGAGA AACGAAGCTT GGATAGTCGG GGAGAGACAA CAGGGAGTGG GCGAGCCATC 
CCCATCAAAC AGAGCTTCCT ACTAAAACGA AGTGGCAATT CCTTGAACAA AGAATGGAAG 
AAGAAATATG TAACCCTGTC CAGTAATGGC TTTCTACTCT ACCACCCCAG TATTAACGAT 
TACATCCACA GTACCCACGG CAAGGAGATG GACTTGCTGC GAACAACAGT CAAAGTCCCG 
GGCAAGCGGC CCCCGAGGGC CATCTCTGCC TTTGGCCCCT CAGCCAGCAT TAACGGGCTC 
GTCAAGGACA TGAGCACTGT CCAGATGGGT GAAGGCCTGG AAGCCACTAC TCCCATGCCA 
AGCCCTAGCC CCAGCCCCAG TTCCCTGCAG CCACCACCAG ATCAGACATC CAAACACCTG 
CTGAAGCCAG ACCGGAATTT GGCCCGAGCC CTCAGCACGG ACTGTACCCC ATCTGGAGAC 
CTGAGCCCCC TGAGTCGGGA ACCCCCTCCT TCTCCCATGG TGAAGAAGCA GAGGAGGAAA 
AAATTGACAA CACCATCCAA GACTGAAGGC TCGGCTGGGC AGGCTGAAGA GGAAAACTTT 
GAGTTCCTGA TCGTGTCCAG CACGGGTCAG ACGTGGCACT TTGAGGCAGC CAGTTTTGAG 
GAGCGGGATG CCTGGGTCCA GGCCATCGAG AGTCAGATCC TAGCCAGTCT GCAATGCTGT 
GAGAGCAGCA AGGTCAAGCT GCGCACAGAC AGCCAAAGCG AGGCCGTGGC CATCCAGGCG 
ATCCGGAACG CCAAGGGGAA TTCAATCTGC GTGGACTGCG GGGCCCCCAA CCCCACGTGG 
GCCAGCTTGA ACCTGGGCGC CCTCATCTGC ATCGAGTGTT CTGGCATCCA CCGCAACCTG 
GGCACACACC TGTCCCGCGT TCGCTCGCTG GACTTGGACG ACTGGCCACG GGAGCTGACC 
CTGGTGCTGA CGGCTATTGG CAACGACACG GCCAACCGCG TGTGGGAAAG CGACACGCGA 
GGCCGTGCCA AGCCCTCGCG GGACTCTTCG CGGGAGGAGC GCGAGTCGTG GATTCGCGCC 
AAGTACGAGC AGCTACTGTT CCTGGCGCCG CTGAGCACCT CGGAGGAGCC GCTGGGCCGC 
CAGCTGTGGG CCGCCGTGCA GGCCCAGGAC GTGGCTACCG TTCTCCTGCT TTTGGCCCAT 
GCGCGACACG GGCCGCTCGA CACCAGCGTA GAGGACCCAC AGCTGCGCTC CCCACTCCAC 
CTGGCGGCCG AGCTCGCCCA CGTCGTCATC ACGCAACTGC TGCTGTGGTA CGGCGCGGAC 
GTGGCGGCCC GTGACGCCCA GGGCCGCACG GCGCTGTTCT ACGCCCGCCA GGCTGGAAGC 
CAGCTGTGCG CCGACATCCT TCTCCAGCAC GGCTGCCCGG GTGAGGGCGG CAGCGCGGCC 
ACCACGCCCA GCGCGGCCAC CACGCCCAGC ATCACCGCCA CGCCCAGCCC CCGCCGCCGG 
AGCAGCGCCG CTAGCGTGGG CCGCGCCGAC GCCCCGGTTG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055585.1
CDS	91..2601
Translation	MHAQRQFVVAAVRAEVRRHEVAKQALNRLRKLAERVDDPELQDSIQASLDSIREAVINSQEWTLSRSIPELRLGVLGDARSGKSSLIHRFLTGSYQVLEKTESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDENSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCADMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGLEATTPMPSPSPSPSSLQPPPDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLTTPSKTEGSAGQAEEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSICVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPSRDSSREERESWIRAKYEQLLFLAPLSTSEEPLGRQLWAAVQAQDVATVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSAATTPSAATTPSITATPSPRRRSSAASVGRADAPVALV

Target ORF information:

RefSeq Version	NM_014770.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014770.4

ORF Insert Sequence:

ATGCATGCCC AGAGGCAGTT CGTTGTAGCT GCAGTGAGAG CAGAAGTCAG ACGACATGAG 
GTGGCCAAGC AGGCTCTAAA CCGCCTCAGG AAGCTGGCAG AGAGGGTGGA CGACCCCGAA 
CTCCAGGACA GCATCCAGGC CTCATTGGAC AGCATTCGAG AGGCTGTGAT CAATAGCCAG 
GAATGGACTT TGAGCCGCTC CATTCCTGAA CTGCGCCTGG GTGTGCTGGG CGATGCCAGG 
AGTGGGAAGT CATCGCTCAT CCACCGATTC CTGACTGGCT CATACCAGGT GCTGGAGAAG 
ACAGAGAGTG AGCAGTACAA GAAAGAAATG TTGGTGGATG GACAGACACA TCTGGTGCTA 
ATCCGAGAGG AAGCTGGGGC ACCTGATGCC AAGTTCTCAG GCTGGGCAGA TGCTGTGATC 
TTCGTCTTCA GCCTGGAGGA TGAGAACAGT TTCCAGGCTG TGAGCCGTCT CCATGGGCAG 
CTGAGTTCCC TTCGCGGGGA GGGACGAGGA GGCCTGGCCT TGGCACTGGT GGGGACACAA 
GACAGGATCA GTGCTTCCTC CCCTCGGGTG GTGGGAGATG CTCGTGCCAG AGCTCTGTGC 
GCGGACATGA AACGCTGCAG CTACTATGAG ACTTGTGCAA CCTATGGGCT CAATGTGGAT 
CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG GTGACCTTGC GCAAGCAGCA ACAGCTTCTG 
GCTGCCTGCA AGTCCCTGCC CAGCTCCCCA AGCCACTCAG CTGCATCCAC TCCGGTAGCT 
GGCCAGGCTA GTAACGGGGG CCACACTAGC GACTACTCTT CTTCCCTCCC GTCCTCACCG 
AATGTTGGTC ACCGGGAGCT CCGAGCCGAG GCAGCTGCAG TGGCTGGATT GAGCACCCCA 
GGGTCCCTGC ACCGGGCAGC CAAGCGCAGG ACCAGCCTTT TTGCGAATCG TCGGGGTAGT 
GACTCCGAGA AACGAAGCTT GGATAGTCGG GGAGAGACAA CAGGGAGTGG GCGAGCCATC 
CCCATCAAAC AGAGCTTCCT ACTAAAACGA AGTGGCAATT CCTTGAACAA AGAATGGAAG 
AAGAAATATG TAACCCTGTC CAGTAATGGC TTTCTACTCT ACCACCCCAG TATTAACGAT 
TACATCCACA GTACCCACGG CAAGGAGATG GACTTGCTGC GAACAACAGT CAAAGTCCCG 
GGCAAGCGGC CCCCGAGGGC CATCTCTGCC TTTGGCCCCT CAGCCAGCAT TAACGGGCTC 
GTCAAGGACA TGAGCACTGT CCAGATGGGT GAAGGCCTGG AAGCCACTAC TCCCATGCCA 
AGCCCTAGCC CCAGCCCCAG TTCCCTGCAG CCACCACCAG ATCAGACATC CAAACACCTG 
CTGAAGCCAG ACCGGAATTT GGCCCGAGCC CTCAGCACGG ACTGTACCCC ATCTGGAGAC 
CTGAGCCCCC TGAGTCGGGA ACCCCCTCCT TCTCCCATGG TGAAGAAGCA GAGGAGGAAA 
AAATTGACAA CACCATCCAA GACTGAAGGC TCGGCTGGGC AGGCTGAAGA GGAAAACTTT 
GAGTTCCTGA TCGTGTCCAG CACGGGTCAG ACGTGGCACT TTGAGGCAGC CAGTTTTGAG 
GAGCGGGATG CCTGGGTCCA GGCCATCGAG AGTCAGATCC TAGCCAGTCT GCAATGCTGT 
GAGAGCAGCA AGGTCAAGCT GCGCACAGAC AGCCAAAGCG AGGCCGTGGC CATCCAGGCG 
ATCCGGAACG CCAAGGGGAA TTCAATCTGC GTGGACTGCG GGGCCCCCAA CCCCACGTGG 
GCCAGCTTGA ACCTGGGCGC CCTCATCTGC ATCGAGTGTT CTGGCATCCA CCGCAACCTG 
GGCACACACC TGTCCCGCGT TCGCTCGCTG GACTTGGACG ACTGGCCACG GGAGCTGACC 
CTGGTGCTGA CGGCTATTGG CAACGACACG GCCAACCGCG TGTGGGAAAG CGACACGCGA 
GGCCGTGCCA AGCCCTCGCG GGACTCTTCG CGGGAGGAGC GCGAGTCGTG GATTCGCGCC 
AAGTACGAGC AGCTACTGTT CCTGGCGCCG CTGAGCACCT CGGAGGAGCC GCTGGGCCGC 
CAGCTGTGGG CCGCCGTGCA GGCCCAGGAC GTGGCTACCG TTCTCCTGCT TTTGGCCCAT 
GCGCGACACG GGCCGCTCGA CACCAGCGTA GAGGACCCAC AGCTGCGCTC CCCACTCCAC 
CTGGCGGCCG AGCTCGCCCA CGTCGTCATC ACGCAACTGC TGCTGTGGTA CGGCGCGGAC 
GTGGCGGCCC GTGACGCCCA GGGCCGCACG GCGCTGTTCT ACGCCCGCCA GGCTGGAAGC 
CAGCTGTGCG CCGACATCCT TCTCCAGCAC GGCTGCCCGG GTGAGGGCGG CAGCGCGGCC 
ACCACGCCCA GCGCGGCCAC CACGCCCAGC ATCACCGCCA CGCCCAGCCC CCGCCGCCGG 
AGCAGCGCCG CTAGCGTGGG CCGCGCCGAC GCCCCGGTTG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGAP2 ( NM_014770.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055585.1 Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18742
Clone ID Related Accession (Same CDS sequence)	NM_014770.4 , NM_014770.3
Accession Version	NM_014770.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform PIKE-A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB165417.1, D79989.1, BI755875.1, BE280072.1 and AV740795.1. This sequence is a reference standard in the RefSeqGene project. On Aug 13, 2011 this sequence version replaced NM_014770.2. Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. This results in a shorter isoform (PIKE-A) with a distinct N-terminus compared to isoform PIKE-L. This isoform is reported to be overexpressed in brain tumors and promote cancer cell invasion (PMID:14761976). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D79989.1, AF384128.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATGCCC AGAGGCAGTT CGTTGTAGCT GCAGTGAGAG CAGAAGTCAG ACGACATGAG 
GTGGCCAAGC AGGCTCTAAA CCGCCTCAGG AAGCTGGCAG AGAGGGTGGA CGACCCCGAA 
CTCCAGGACA GCATCCAGGC CTCATTGGAC AGCATTCGAG AGGCTGTGAT CAATAGCCAG 
GAATGGACTT TGAGCCGCTC CATTCCTGAA CTGCGCCTGG GTGTGCTGGG CGATGCCAGG 
AGTGGGAAGT CATCGCTCAT CCACCGATTC CTGACTGGCT CATACCAGGT GCTGGAGAAG 
ACAGAGAGTG AGCAGTACAA GAAAGAAATG TTGGTGGATG GACAGACACA TCTGGTGCTA 
ATCCGAGAGG AAGCTGGGGC ACCTGATGCC AAGTTCTCAG GCTGGGCAGA TGCTGTGATC 
TTCGTCTTCA GCCTGGAGGA TGAGAACAGT TTCCAGGCTG TGAGCCGTCT CCATGGGCAG 
CTGAGTTCCC TTCGCGGGGA GGGACGAGGA GGCCTGGCCT TGGCACTGGT GGGGACACAA 
GACAGGATCA GTGCTTCCTC CCCTCGGGTG GTGGGAGATG CTCGTGCCAG AGCTCTGTGC 
GCGGACATGA AACGCTGCAG CTACTATGAG ACTTGTGCAA CCTATGGGCT CAATGTGGAT 
CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG GTGACCTTGC GCAAGCAGCA ACAGCTTCTG 
GCTGCCTGCA AGTCCCTGCC CAGCTCCCCA AGCCACTCAG CTGCATCCAC TCCGGTAGCT 
GGCCAGGCTA GTAACGGGGG CCACACTAGC GACTACTCTT CTTCCCTCCC GTCCTCACCG 
AATGTTGGTC ACCGGGAGCT CCGAGCCGAG GCAGCTGCAG TGGCTGGATT GAGCACCCCA 
GGGTCCCTGC ACCGGGCAGC CAAGCGCAGG ACCAGCCTTT TTGCGAATCG TCGGGGTAGT 
GACTCCGAGA AACGAAGCTT GGATAGTCGG GGAGAGACAA CAGGGAGTGG GCGAGCCATC 
CCCATCAAAC AGAGCTTCCT ACTAAAACGA AGTGGCAATT CCTTGAACAA AGAATGGAAG 
AAGAAATATG TAACCCTGTC CAGTAATGGC TTTCTACTCT ACCACCCCAG TATTAACGAT 
TACATCCACA GTACCCACGG CAAGGAGATG GACTTGCTGC GAACAACAGT CAAAGTCCCG 
GGCAAGCGGC CCCCGAGGGC CATCTCTGCC TTTGGCCCCT CAGCCAGCAT TAACGGGCTC 
GTCAAGGACA TGAGCACTGT CCAGATGGGT GAAGGCCTGG AAGCCACTAC TCCCATGCCA 
AGCCCTAGCC CCAGCCCCAG TTCCCTGCAG CCACCACCAG ATCAGACATC CAAACACCTG 
CTGAAGCCAG ACCGGAATTT GGCCCGAGCC CTCAGCACGG ACTGTACCCC ATCTGGAGAC 
CTGAGCCCCC TGAGTCGGGA ACCCCCTCCT TCTCCCATGG TGAAGAAGCA GAGGAGGAAA 
AAATTGACAA CACCATCCAA GACTGAAGGC TCGGCTGGGC AGGCTGAAGA GGAAAACTTT 
GAGTTCCTGA TCGTGTCCAG CACGGGTCAG ACGTGGCACT TTGAGGCAGC CAGTTTTGAG 
GAGCGGGATG CCTGGGTCCA GGCCATCGAG AGTCAGATCC TAGCCAGTCT GCAATGCTGT 
GAGAGCAGCA AGGTCAAGCT GCGCACAGAC AGCCAAAGCG AGGCCGTGGC CATCCAGGCG 
ATCCGGAACG CCAAGGGGAA TTCAATCTGC GTGGACTGCG GGGCCCCCAA CCCCACGTGG 
GCCAGCTTGA ACCTGGGCGC CCTCATCTGC ATCGAGTGTT CTGGCATCCA CCGCAACCTG 
GGCACACACC TGTCCCGCGT TCGCTCGCTG GACTTGGACG ACTGGCCACG GGAGCTGACC 
CTGGTGCTGA CGGCTATTGG CAACGACACG GCCAACCGCG TGTGGGAAAG CGACACGCGA 
GGCCGTGCCA AGCCCTCGCG GGACTCTTCG CGGGAGGAGC GCGAGTCGTG GATTCGCGCC 
AAGTACGAGC AGCTACTGTT CCTGGCGCCG CTGAGCACCT CGGAGGAGCC GCTGGGCCGC 
CAGCTGTGGG CCGCCGTGCA GGCCCAGGAC GTGGCTACCG TTCTCCTGCT TTTGGCCCAT 
GCGCGACACG GGCCGCTCGA CACCAGCGTA GAGGACCCAC AGCTGCGCTC CCCACTCCAC 
CTGGCGGCCG AGCTCGCCCA CGTCGTCATC ACGCAACTGC TGCTGTGGTA CGGCGCGGAC 
GTGGCGGCCC GTGACGCCCA GGGCCGCACG GCGCTGTTCT ACGCCCGCCA GGCTGGAAGC 
CAGCTGTGCG CCGACATCCT TCTCCAGCAC GGCTGCCCGG GTGAGGGCGG CAGCGCGGCC 
ACCACGCCCA GCGCGGCCAC CACGCCCAGC ATCACCGCCA CGCCCAGCCC CCGCCGCCGG 
AGCAGCGCCG CTAGCGTGGG CCGCGCCGAC GCCCCGGTTG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055585.1
CDS	91..2601
Translation	MHAQRQFVVAAVRAEVRRHEVAKQALNRLRKLAERVDDPELQDSIQASLDSIREAVINSQEWTLSRSIPELRLGVLGDARSGKSSLIHRFLTGSYQVLEKTESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDENSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCADMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGLEATTPMPSPSPSPSSLQPPPDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLTTPSKTEGSAGQAEEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSICVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPSRDSSREERESWIRAKYEQLLFLAPLSTSEEPLGRQLWAAVQAQDVATVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSAATTPSAATTPSITATPSPRRRSSAASVGRADAPVALV

Target ORF information:

RefSeq Version	NM_014770.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014770.3

ORF Insert Sequence:

ATGCATGCCC AGAGGCAGTT CGTTGTAGCT GCAGTGAGAG CAGAAGTCAG ACGACATGAG 
GTGGCCAAGC AGGCTCTAAA CCGCCTCAGG AAGCTGGCAG AGAGGGTGGA CGACCCCGAA 
CTCCAGGACA GCATCCAGGC CTCATTGGAC AGCATTCGAG AGGCTGTGAT CAATAGCCAG 
GAATGGACTT TGAGCCGCTC CATTCCTGAA CTGCGCCTGG GTGTGCTGGG CGATGCCAGG 
AGTGGGAAGT CATCGCTCAT CCACCGATTC CTGACTGGCT CATACCAGGT GCTGGAGAAG 
ACAGAGAGTG AGCAGTACAA GAAAGAAATG TTGGTGGATG GACAGACACA TCTGGTGCTA 
ATCCGAGAGG AAGCTGGGGC ACCTGATGCC AAGTTCTCAG GCTGGGCAGA TGCTGTGATC 
TTCGTCTTCA GCCTGGAGGA TGAGAACAGT TTCCAGGCTG TGAGCCGTCT CCATGGGCAG 
CTGAGTTCCC TTCGCGGGGA GGGACGAGGA GGCCTGGCCT TGGCACTGGT GGGGACACAA 
GACAGGATCA GTGCTTCCTC CCCTCGGGTG GTGGGAGATG CTCGTGCCAG AGCTCTGTGC 
GCGGACATGA AACGCTGCAG CTACTATGAG ACTTGTGCAA CCTATGGGCT CAATGTGGAT 
CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG GTGACCTTGC GCAAGCAGCA ACAGCTTCTG 
GCTGCCTGCA AGTCCCTGCC CAGCTCCCCA AGCCACTCAG CTGCATCCAC TCCGGTAGCT 
GGCCAGGCTA GTAACGGGGG CCACACTAGC GACTACTCTT CTTCCCTCCC GTCCTCACCG 
AATGTTGGTC ACCGGGAGCT CCGAGCCGAG GCAGCTGCAG TGGCTGGATT GAGCACCCCA 
GGGTCCCTGC ACCGGGCAGC CAAGCGCAGG ACCAGCCTTT TTGCGAATCG TCGGGGTAGT 
GACTCCGAGA AACGAAGCTT GGATAGTCGG GGAGAGACAA CAGGGAGTGG GCGAGCCATC 
CCCATCAAAC AGAGCTTCCT ACTAAAACGA AGTGGCAATT CCTTGAACAA AGAATGGAAG 
AAGAAATATG TAACCCTGTC CAGTAATGGC TTTCTACTCT ACCACCCCAG TATTAACGAT 
TACATCCACA GTACCCACGG CAAGGAGATG GACTTGCTGC GAACAACAGT CAAAGTCCCG 
GGCAAGCGGC CCCCGAGGGC CATCTCTGCC TTTGGCCCCT CAGCCAGCAT TAACGGGCTC 
GTCAAGGACA TGAGCACTGT CCAGATGGGT GAAGGCCTGG AAGCCACTAC TCCCATGCCA 
AGCCCTAGCC CCAGCCCCAG TTCCCTGCAG CCACCACCAG ATCAGACATC CAAACACCTG 
CTGAAGCCAG ACCGGAATTT GGCCCGAGCC CTCAGCACGG ACTGTACCCC ATCTGGAGAC 
CTGAGCCCCC TGAGTCGGGA ACCCCCTCCT TCTCCCATGG TGAAGAAGCA GAGGAGGAAA 
AAATTGACAA CACCATCCAA GACTGAAGGC TCGGCTGGGC AGGCTGAAGA GGAAAACTTT 
GAGTTCCTGA TCGTGTCCAG CACGGGTCAG ACGTGGCACT TTGAGGCAGC CAGTTTTGAG 
GAGCGGGATG CCTGGGTCCA GGCCATCGAG AGTCAGATCC TAGCCAGTCT GCAATGCTGT 
GAGAGCAGCA AGGTCAAGCT GCGCACAGAC AGCCAAAGCG AGGCCGTGGC CATCCAGGCG 
ATCCGGAACG CCAAGGGGAA TTCAATCTGC GTGGACTGCG GGGCCCCCAA CCCCACGTGG 
GCCAGCTTGA ACCTGGGCGC CCTCATCTGC ATCGAGTGTT CTGGCATCCA CCGCAACCTG 
GGCACACACC TGTCCCGCGT TCGCTCGCTG GACTTGGACG ACTGGCCACG GGAGCTGACC 
CTGGTGCTGA CGGCTATTGG CAACGACACG GCCAACCGCG TGTGGGAAAG CGACACGCGA 
GGCCGTGCCA AGCCCTCGCG GGACTCTTCG CGGGAGGAGC GCGAGTCGTG GATTCGCGCC 
AAGTACGAGC AGCTACTGTT CCTGGCGCCG CTGAGCACCT CGGAGGAGCC GCTGGGCCGC 
CAGCTGTGGG CCGCCGTGCA GGCCCAGGAC GTGGCTACCG TTCTCCTGCT TTTGGCCCAT 
GCGCGACACG GGCCGCTCGA CACCAGCGTA GAGGACCCAC AGCTGCGCTC CCCACTCCAC 
CTGGCGGCCG AGCTCGCCCA CGTCGTCATC ACGCAACTGC TGCTGTGGTA CGGCGCGGAC 
GTGGCGGCCC GTGACGCCCA GGGCCGCACG GCGCTGTTCT ACGCCCGCCA GGCTGGAAGC 
CAGCTGTGCG CCGACATCCT TCTCCAGCAC GGCTGCCCGG GTGAGGGCGG CAGCGCGGCC 
ACCACGCCCA GCGCGGCCAC CACGCCCAGC ATCACCGCCA CGCCCAGCCC CCGCCGCCGG 
AGCAGCGCCG CTAGCGTGGG CCGCGCCGAC GCCCCGGTTG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGAP2 ( NM_001122772.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001116244.1 Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 1, mRNA.

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CloneID	OHu11929
Clone ID Related Accession (Same CDS sequence)	XM_017018772.1 , XM_017018772.2 , NM_001122772.2
Accession Version	NM_001122772.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform PIKE-L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY128689.1, AC025165.27, D79989.1, BI755875.1, BE280072.1 and AV740795.1. On Aug 13, 2011 this sequence version replaced NM_001122772.1. Transcript Variant: This variant (1) encodes the longer isoform (PIKE-L). This isoform is thought to participate in the prevention of neuronal apoptosis by enhancing nuclear phosphoinositide 3-kinase activity (PMID:14528310). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY128689.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCCGGG GCGCGGGCGC GCTTCAGCGC CGGACAACGA CCTACCTCAT CTCGCTGACC 
CTGGTTAAGC TCGAGTCGGT GCCTCCGCCG CCGCCTTCTC CGTCTGCGGC CGCGGCCGGC 
GCCGCCGGTG CCAGAGGCTC CGAGACTGGG GATCCTGGCA GCCCCCGAGG CGCGGAGGAG 
CCGGGCAAGA AGCGGCACGA ACGTCTCTTC CACCGGCAGG ATGCGCTGTG GATCAGCACG 
AGCAGCGCGG GCACCGGGGG CGCGGAGCCC CCAGCCCTGT CCCCGGCTCC GGCCAGTCCG 
GCCCGCCCAG TCTCCCCCGC TCCCGGCCGC CGCCTCTCCC TCTGGGCCGT CCCTCCGGGA 
CCCCCGCTCT CCGGGGGACT GAGCCCCGAC CCCAAGCCTG GGGGCGCCCC CACCTCCTCC 
CGGCGCCCCC TGCTCAGCAG CCCGAGCTGG GGCGGCCCGG AGCCCGAAGG CCGGGCGGGC 
GGCGGCATCC CTGGCTCATC CTCTCCGCAC CCTGGCACCG GCAGCCGGAG GCTCAAGGTG 
GCGCCTCCTC CGCCGGCTCC CAAGCCTTGC AAGACCGTGA CCACGAGTGG AGCCAAAGCC 
GGCGGGGGCA AGGGCGCGGG TAGCCGCCTG TCATGGCCCG AAAGCGAGGG CAAGCCCAGG 
GTCAAGGGGT CAAAGAGCAG CGCCGGGACT GGAGCTTCGG TCTCTGCCGC CGCCACCGCC 
GCCGCCGCCG GGGGAGGGGG CTCTACAGCT TCGACCTCTG GTGGGGTCGG GGCTGGGGCT 
GGAGCCCGAG GGAAGTTGTC CCCTCGGAAA GGCAAGAGTA AGACCTTGGA CAACAGTGAC 
TTGCATCCGG GACCGCCTGC CGGCTCTCCT CCTCCGCTAA CCCTCCCACC AACTCCGAGT 
CCAGCCACTG CTGTCACCGC TGCTTCCGCG CAGCCCCCCG GGCCTGCACC TCCAATCACT 
CTGGAGCCTC CAGCTCCGGG GCTGAAACGG GGCCGGGAGG GGGGCCGAGC ATCCACTCGT 
GACCGCAAGA TGCTCAAGTT TATCAGCGGC ATCTTCACCA AGAGCACAGG AGGGCCTCCT 
GGCTCCGGGC CCCTTCCCGG ACCCCCCAGC CTGTCTTCTG GCAGCGGGTC CAGGGAGCTG 
CTGGGCGCCG AGCTCCGCGC TTCCCCTAAG GCTGTGATCA ATAGCCAGGA ATGGACTTTG 
AGCCGCTCCA TTCCTGAACT GCGCCTGGGT GTGCTGGGCG ATGCCAGGAG TGGGAAGTCA 
TCGCTCATCC ACCGATTCCT GACTGGCTCA TACCAGGTGC TGGAGAAGAC AGAGAGTGAG 
CAGTACAAGA AAGAAATGTT GGTGGATGGA CAGACACATC TGGTGCTAAT CCGAGAGGAA 
GCTGGGGCAC CTGATGCCAA GTTCTCAGGC TGGGCAGATG CTGTGATCTT CGTCTTCAGC 
CTGGAGGATG AGAACAGTTT CCAGGCTGTG AGCCGTCTCC ATGGGCAGCT GAGTTCCCTT 
CGCGGGGAGG GACGAGGAGG CCTGGCCTTG GCACTGGTGG GGACACAAGA CAGGATCAGT 
GCTTCCTCCC CTCGGGTGGT GGGAGATGCT CGTGCCAGAG CTCTGTGCGC GGACATGAAA 
CGCTGCAGCT ACTATGAGAC TTGTGCAACC TATGGGCTCA ATGTGGATCG GGTCTTCCAG 
GAGGTGGCCC AGAAGGTGGT GACCTTGCGC AAGCAGCAAC AGCTTCTGGC TGCCTGCAAG 
TCCCTGCCCA GCTCCCCAAG CCACTCAGCT GCATCCACTC CGGTAGCTGG CCAGGCTAGT 
AACGGGGGCC ACACTAGCGA CTACTCTTCT TCCCTCCCGT CCTCACCGAA TGTTGGTCAC 
CGGGAGCTCC GAGCCGAGGC AGCTGCAGTG GCTGGATTGA GCACCCCAGG GTCCCTGCAC 
CGGGCAGCCA AGCGCAGGAC CAGCCTTTTT GCGAATCGTC GGGGTAGTGA CTCCGAGAAA 
CGAAGCTTGG ATAGTCGGGG AGAGACAACA GGGAGTGGGC GAGCCATCCC CATCAAACAG 
AGCTTCCTAC TAAAACGAAG TGGCAATTCC TTGAACAAAG AATGGAAGAA GAAATATGTA 
ACCCTGTCCA GTAATGGCTT TCTACTCTAC CACCCCAGTA TTAACGATTA CATCCACAGT 
ACCCACGGCA AGGAGATGGA CTTGCTGCGA ACAACAGTCA AAGTCCCGGG CAAGCGGCCC 
CCGAGGGCCA TCTCTGCCTT TGGCCCCTCA GCCAGCATTA ACGGGCTCGT CAAGGACATG 
AGCACTGTCC AGATGGGTGA AGGCCTGGAA GCCACTACTC CCATGCCAAG CCCTAGCCCC 
AGCCCCAGTT CCCTGCAGCC ACCACCAGAT CAGACATCCA AACACCTGCT GAAGCCAGAC 
CGGAATTTGG CCCGAGCCCT CAGCACGGAC TGTACCCCAT CTGGAGACCT GAGCCCCCTG 
AGTCGGGAAC CCCCTCCTTC TCCCATGGTG AAGAAGCAGA GGAGGAAAAA ATTGACAACA 
CCATCCAAGA CTGAAGGCTC GGCTGGGCAG GCTGAAGCCA AGCGCAAAAT GTGGAAACTA 
AAATCCTTTG GTAGTTTAAG AAATATTTAT AAAGCAGAGG AAAACTTTGA GTTCCTGATC 
GTGTCCAGCA CGGGTCAGAC GTGGCACTTT GAGGCAGCCA GTTTTGAGGA GCGGGATGCC 
TGGGTCCAGG CCATCGAGAG TCAGATCCTA GCCAGTCTGC AATGCTGTGA GAGCAGCAAG 
GTCAAGCTGC GCACAGACAG CCAAAGCGAG GCCGTGGCCA TCCAGGCGAT CCGGAACGCC 
AAGGGGAATT CAATCTGCGT GGACTGCGGG GCCCCCAACC CCACGTGGGC CAGCTTGAAC 
CTGGGCGCCC TCATCTGCAT CGAGTGTTCT GGCATCCACC GCAACCTGGG CACACACCTG 
TCCCGCGTTC GCTCGCTGGA CTTGGACGAC TGGCCACGGG AGCTGACCCT GGTGCTGACG 
GCTATTGGCA ACGACACGGC CAACCGCGTG TGGGAAAGCG ACACGCGAGG CCGTGCCAAG 
CCCTCGCGGG ACTCTTCGCG GGAGGAGCGC GAGTCGTGGA TTCGCGCCAA GTACGAGCAG 
CTACTGTTCC TGGCGCCGCT GAGCACCTCG GAGGAGCCGC TGGGCCGCCA GCTGTGGGCC 
GCCGTGCAGG CCCAGGACGT GGCTACCGTT CTCCTGCTTT TGGCCCATGC GCGACACGGG 
CCGCTCGACA CCAGCGTAGA GGACCCACAG CTGCGCTCCC CACTCCACCT GGCGGCCGAG 
CTCGCCCACG TCGTCATCAC GCAACTGCTG CTGTGGTACG GCGCGGACGT GGCGGCCCGT 
GACGCCCAGG GCCGCACGGC GCTGTTCTAC GCCCGCCAGG CTGGAAGCCA GCTGTGCGCC 
GACATCCTTC TCCAGCACGG CTGCCCGGGT GAGGGCGGCA GCGCGGCCAC CACGCCCAGC 
GCGGCCACCA CGCCCAGCAT CACCGCCACG CCCAGCCCCC GCCGCCGGAG CAGCGCCGCT 
AGCGTGGGCC GCGCCGACGC CCCGGTTGCG CTGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116244.1
CDS	1..3579
Translation	MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAAGAAGARGSETGDPGSPRGAEEPGKKRHERLFHRQDALWISTSSAGTGGAEPPALSPAPASPARPVSPAPGRRLSLWAVPPGPPLSGGLSPDPKPGGAPTSSRRPLLSSPSWGGPEPEGRAGGGIPGSSSPHPGTGSRRLKVAPPPPAPKPCKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSSAGTGASVSAAATAAAAGGGGSTASTSGGVGAGAGARGKLSPRKGKSKTLDNSDLHPGPPAGSPPPLTLPPTPSPATAVTAASAQPPGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGSGPLPGPPSLSSGSGSRELLGAELRASPKAVINSQEWTLSRSIPELRLGVLGDARSGKSSLIHRFLTGSYQVLEKTESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDENSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCADMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGLEATTPMPSPSPSPSSLQPPPDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLTTPSKTEGSAGQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSICVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPSRDSSREERESWIRAKYEQLLFLAPLSTSEEPLGRQLWAAVQAQDVATVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSAATTPSAATTPSITATPSPRRRSSAASVGRADAPVALV

Target ORF information:

RefSeq Version	NM_001122772.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122772.2

ORF Insert Sequence:

ATGAGCCGGG GCGCGGGCGC GCTTCAGCGC CGGACAACGA CCTACCTCAT CTCGCTGACC 
CTGGTTAAGC TCGAGTCGGT GCCTCCGCCG CCGCCTTCTC CGTCTGCGGC CGCGGCCGGC 
GCCGCCGGTG CCAGAGGCTC CGAGACTGGG GATCCTGGCA GCCCCCGAGG CGCGGAGGAG 
CCGGGCAAGA AGCGGCACGA ACGTCTCTTC CACCGGCAGG ATGCGCTGTG GATCAGCACG 
AGCAGCGCGG GCACCGGGGG CGCGGAGCCC CCAGCCCTGT CCCCGGCTCC GGCCAGTCCG 
GCCCGCCCAG TCTCCCCCGC TCCCGGCCGC CGCCTCTCCC TCTGGGCCGT CCCTCCGGGA 
CCCCCGCTCT CCGGGGGACT GAGCCCCGAC CCCAAGCCTG GGGGCGCCCC CACCTCCTCC 
CGGCGCCCCC TGCTCAGCAG CCCGAGCTGG GGCGGCCCGG AGCCCGAAGG CCGGGCGGGC 
GGCGGCATCC CTGGCTCATC CTCTCCGCAC CCTGGCACCG GCAGCCGGAG GCTCAAGGTG 
GCGCCTCCTC CGCCGGCTCC CAAGCCTTGC AAGACCGTGA CCACGAGTGG AGCCAAAGCC 
GGCGGGGGCA AGGGCGCGGG TAGCCGCCTG TCATGGCCCG AAAGCGAGGG CAAGCCCAGG 
GTCAAGGGGT CAAAGAGCAG CGCCGGGACT GGAGCTTCGG TCTCTGCCGC CGCCACCGCC 
GCCGCCGCCG GGGGAGGGGG CTCTACAGCT TCGACCTCTG GTGGGGTCGG GGCTGGGGCT 
GGAGCCCGAG GGAAGTTGTC CCCTCGGAAA GGCAAGAGTA AGACCTTGGA CAACAGTGAC 
TTGCATCCGG GACCGCCTGC CGGCTCTCCT CCTCCGCTAA CCCTCCCACC AACTCCGAGT 
CCAGCCACTG CTGTCACCGC TGCTTCCGCG CAGCCCCCCG GGCCTGCACC TCCAATCACT 
CTGGAGCCTC CAGCTCCGGG GCTGAAACGG GGCCGGGAGG GGGGCCGAGC ATCCACTCGT 
GACCGCAAGA TGCTCAAGTT TATCAGCGGC ATCTTCACCA AGAGCACAGG AGGGCCTCCT 
GGCTCCGGGC CCCTTCCCGG ACCCCCCAGC CTGTCTTCTG GCAGCGGGTC CAGGGAGCTG 
CTGGGCGCCG AGCTCCGCGC TTCCCCTAAG GCTGTGATCA ATAGCCAGGA ATGGACTTTG 
AGCCGCTCCA TTCCTGAACT GCGCCTGGGT GTGCTGGGCG ATGCCAGGAG TGGGAAGTCA 
TCGCTCATCC ACCGATTCCT GACTGGCTCA TACCAGGTGC TGGAGAAGAC AGAGAGTGAG 
CAGTACAAGA AAGAAATGTT GGTGGATGGA CAGACACATC TGGTGCTAAT CCGAGAGGAA 
GCTGGGGCAC CTGATGCCAA GTTCTCAGGC TGGGCAGATG CTGTGATCTT CGTCTTCAGC 
CTGGAGGATG AGAACAGTTT CCAGGCTGTG AGCCGTCTCC ATGGGCAGCT GAGTTCCCTT 
CGCGGGGAGG GACGAGGAGG CCTGGCCTTG GCACTGGTGG GGACACAAGA CAGGATCAGT 
GCTTCCTCCC CTCGGGTGGT GGGAGATGCT CGTGCCAGAG CTCTGTGCGC GGACATGAAA 
CGCTGCAGCT ACTATGAGAC TTGTGCAACC TATGGGCTCA ATGTGGATCG GGTCTTCCAG 
GAGGTGGCCC AGAAGGTGGT GACCTTGCGC AAGCAGCAAC AGCTTCTGGC TGCCTGCAAG 
TCCCTGCCCA GCTCCCCAAG CCACTCAGCT GCATCCACTC CGGTAGCTGG CCAGGCTAGT 
AACGGGGGCC ACACTAGCGA CTACTCTTCT TCCCTCCCGT CCTCACCGAA TGTTGGTCAC 
CGGGAGCTCC GAGCCGAGGC AGCTGCAGTG GCTGGATTGA GCACCCCAGG GTCCCTGCAC 
CGGGCAGCCA AGCGCAGGAC CAGCCTTTTT GCGAATCGTC GGGGTAGTGA CTCCGAGAAA 
CGAAGCTTGG ATAGTCGGGG AGAGACAACA GGGAGTGGGC GAGCCATCCC CATCAAACAG 
AGCTTCCTAC TAAAACGAAG TGGCAATTCC TTGAACAAAG AATGGAAGAA GAAATATGTA 
ACCCTGTCCA GTAATGGCTT TCTACTCTAC CACCCCAGTA TTAACGATTA CATCCACAGT 
ACCCACGGCA AGGAGATGGA CTTGCTGCGA ACAACAGTCA AAGTCCCGGG CAAGCGGCCC 
CCGAGGGCCA TCTCTGCCTT TGGCCCCTCA GCCAGCATTA ACGGGCTCGT CAAGGACATG 
AGCACTGTCC AGATGGGTGA AGGCCTGGAA GCCACTACTC CCATGCCAAG CCCTAGCCCC 
AGCCCCAGTT CCCTGCAGCC ACCACCAGAT CAGACATCCA AACACCTGCT GAAGCCAGAC 
CGGAATTTGG CCCGAGCCCT CAGCACGGAC TGTACCCCAT CTGGAGACCT GAGCCCCCTG 
AGTCGGGAAC CCCCTCCTTC TCCCATGGTG AAGAAGCAGA GGAGGAAAAA ATTGACAACA 
CCATCCAAGA CTGAAGGCTC GGCTGGGCAG GCTGAAGCCA AGCGCAAAAT GTGGAAACTA 
AAATCCTTTG GTAGTTTAAG AAATATTTAT AAAGCAGAGG AAAACTTTGA GTTCCTGATC 
GTGTCCAGCA CGGGTCAGAC GTGGCACTTT GAGGCAGCCA GTTTTGAGGA GCGGGATGCC 
TGGGTCCAGG CCATCGAGAG TCAGATCCTA GCCAGTCTGC AATGCTGTGA GAGCAGCAAG 
GTCAAGCTGC GCACAGACAG CCAAAGCGAG GCCGTGGCCA TCCAGGCGAT CCGGAACGCC 
AAGGGGAATT CAATCTGCGT GGACTGCGGG GCCCCCAACC CCACGTGGGC CAGCTTGAAC 
CTGGGCGCCC TCATCTGCAT CGAGTGTTCT GGCATCCACC GCAACCTGGG CACACACCTG 
TCCCGCGTTC GCTCGCTGGA CTTGGACGAC TGGCCACGGG AGCTGACCCT GGTGCTGACG 
GCTATTGGCA ACGACACGGC CAACCGCGTG TGGGAAAGCG ACACGCGAGG CCGTGCCAAG 
CCCTCGCGGG ACTCTTCGCG GGAGGAGCGC GAGTCGTGGA TTCGCGCCAA GTACGAGCAG 
CTACTGTTCC TGGCGCCGCT GAGCACCTCG GAGGAGCCGC TGGGCCGCCA GCTGTGGGCC 
GCCGTGCAGG CCCAGGACGT GGCTACCGTT CTCCTGCTTT TGGCCCATGC GCGACACGGG 
CCGCTCGACA CCAGCGTAGA GGACCCACAG CTGCGCTCCC CACTCCACCT GGCGGCCGAG 
CTCGCCCACG TCGTCATCAC GCAACTGCTG CTGTGGTACG GCGCGGACGT GGCGGCCCGT 
GACGCCCAGG GCCGCACGGC GCTGTTCTAC GCCCGCCAGG CTGGAAGCCA GCTGTGCGCC 
GACATCCTTC TCCAGCACGG CTGCCCGGGT GAGGGCGGCA GCGCGGCCAC CACGCCCAGC 
GCGGCCACCA CGCCCAGCAT CACCGCCACG CCCAGCCCCC GCCGCCGGAG CAGCGCCGCT 
AGCGTGGGCC GCGCCGACGC CCCGGTTGCG CTGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGAP2 ( XM_017018772.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874261.1 Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant X1, mRNA.

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CloneID	OHu11929
Clone ID Related Accession (Same CDS sequence)	XM_017018772.1 , XM_017018772.2 , NM_001122772.2
Accession Version	XM_017018772.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGG GCGCGGGCGC GCTTCAGCGC CGGACAACGA CCTACCTCAT CTCGCTGACC 
CTGGTTAAGC TCGAGTCGGT GCCTCCGCCG CCGCCTTCTC CGTCTGCGGC CGCGGCCGGC 
GCCGCCGGTG CCAGAGGCTC CGAGACTGGG GATCCTGGCA GCCCCCGAGG CGCGGAGGAG 
CCGGGCAAGA AGCGGCACGA ACGTCTCTTC CACCGGCAGG ATGCGCTGTG GATCAGCACG 
AGCAGCGCGG GCACCGGGGG CGCGGAGCCC CCAGCCCTGT CCCCGGCTCC GGCCAGTCCG 
GCCCGCCCAG TCTCCCCCGC TCCCGGCCGC CGCCTCTCCC TCTGGGCCGT CCCTCCGGGA 
CCCCCGCTCT CCGGGGGACT GAGCCCCGAC CCCAAGCCTG GGGGCGCCCC CACCTCCTCC 
CGGCGCCCCC TGCTCAGCAG CCCGAGCTGG GGCGGCCCGG AGCCCGAAGG CCGGGCGGGC 
GGCGGCATCC CTGGCTCATC CTCTCCGCAC CCTGGCACCG GCAGCCGGAG GCTCAAGGTG 
GCGCCTCCTC CGCCGGCTCC CAAGCCTTGC AAGACCGTGA CCACGAGTGG AGCCAAAGCC 
GGCGGGGGCA AGGGCGCGGG TAGCCGCCTG TCATGGCCCG AAAGCGAGGG CAAGCCCAGG 
GTCAAGGGGT CAAAGAGCAG CGCCGGGACT GGAGCTTCGG TCTCTGCCGC CGCCACCGCC 
GCCGCCGCCG GGGGAGGGGG CTCTACAGCT TCGACCTCTG GTGGGGTCGG GGCTGGGGCT 
GGAGCCCGAG GGAAGTTGTC CCCTCGGAAA GGCAAGAGTA AGACCTTGGA CAACAGTGAC 
TTGCATCCGG GACCGCCTGC CGGCTCTCCT CCTCCGCTAA CCCTCCCACC AACTCCGAGT 
CCAGCCACTG CTGTCACCGC TGCTTCCGCG CAGCCCCCCG GGCCTGCACC TCCAATCACT 
CTGGAGCCTC CAGCTCCGGG GCTGAAACGG GGCCGGGAGG GGGGCCGAGC ATCCACTCGT 
GACCGCAAGA TGCTCAAGTT TATCAGCGGC ATCTTCACCA AGAGCACAGG AGGGCCTCCT 
GGCTCCGGGC CCCTTCCCGG ACCCCCCAGC CTGTCTTCTG GCAGCGGGTC CAGGGAGCTG 
CTGGGCGCCG AGCTCCGCGC TTCCCCTAAG GCTGTGATCA ATAGCCAGGA ATGGACTTTG 
AGCCGCTCCA TTCCTGAACT GCGCCTGGGT GTGCTGGGCG ATGCCAGGAG TGGGAAGTCA 
TCGCTCATCC ACCGATTCCT GACTGGCTCA TACCAGGTGC TGGAGAAGAC AGAGAGTGAG 
CAGTACAAGA AAGAAATGTT GGTGGATGGA CAGACACATC TGGTGCTAAT CCGAGAGGAA 
GCTGGGGCAC CTGATGCCAA GTTCTCAGGC TGGGCAGATG CTGTGATCTT CGTCTTCAGC 
CTGGAGGATG AGAACAGTTT CCAGGCTGTG AGCCGTCTCC ATGGGCAGCT GAGTTCCCTT 
CGCGGGGAGG GACGAGGAGG CCTGGCCTTG GCACTGGTGG GGACACAAGA CAGGATCAGT 
GCTTCCTCCC CTCGGGTGGT GGGAGATGCT CGTGCCAGAG CTCTGTGCGC GGACATGAAA 
CGCTGCAGCT ACTATGAGAC TTGTGCAACC TATGGGCTCA ATGTGGATCG GGTCTTCCAG 
GAGGTGGCCC AGAAGGTGGT GACCTTGCGC AAGCAGCAAC AGCTTCTGGC TGCCTGCAAG 
TCCCTGCCCA GCTCCCCAAG CCACTCAGCT GCATCCACTC CGGTAGCTGG CCAGGCTAGT 
AACGGGGGCC ACACTAGCGA CTACTCTTCT TCCCTCCCGT CCTCACCGAA TGTTGGTCAC 
CGGGAGCTCC GAGCCGAGGC AGCTGCAGTG GCTGGATTGA GCACCCCAGG GTCCCTGCAC 
CGGGCAGCCA AGCGCAGGAC CAGCCTTTTT GCGAATCGTC GGGGTAGTGA CTCCGAGAAA 
CGAAGCTTGG ATAGTCGGGG AGAGACAACA GGGAGTGGGC GAGCCATCCC CATCAAACAG 
AGCTTCCTAC TAAAACGAAG TGGCAATTCC TTGAACAAAG AATGGAAGAA GAAATATGTA 
ACCCTGTCCA GTAATGGCTT TCTACTCTAC CACCCCAGTA TTAACGATTA CATCCACAGT 
ACCCACGGCA AGGAGATGGA CTTGCTGCGA ACAACAGTCA AAGTCCCGGG CAAGCGGCCC 
CCGAGGGCCA TCTCTGCCTT TGGCCCCTCA GCCAGCATTA ACGGGCTCGT CAAGGACATG 
AGCACTGTCC AGATGGGTGA AGGCCTGGAA GCCACTACTC CCATGCCAAG CCCTAGCCCC 
AGCCCCAGTT CCCTGCAGCC ACCACCAGAT CAGACATCCA AACACCTGCT GAAGCCAGAC 
CGGAATTTGG CCCGAGCCCT CAGCACGGAC TGTACCCCAT CTGGAGACCT GAGCCCCCTG 
AGTCGGGAAC CCCCTCCTTC TCCCATGGTG AAGAAGCAGA GGAGGAAAAA ATTGACAACA 
CCATCCAAGA CTGAAGGCTC GGCTGGGCAG GCTGAAGCCA AGCGCAAAAT GTGGAAACTA 
AAATCCTTTG GTAGTTTAAG AAATATTTAT AAAGCAGAGG AAAACTTTGA GTTCCTGATC 
GTGTCCAGCA CGGGTCAGAC GTGGCACTTT GAGGCAGCCA GTTTTGAGGA GCGGGATGCC 
TGGGTCCAGG CCATCGAGAG TCAGATCCTA GCCAGTCTGC AATGCTGTGA GAGCAGCAAG 
GTCAAGCTGC GCACAGACAG CCAAAGCGAG GCCGTGGCCA TCCAGGCGAT CCGGAACGCC 
AAGGGGAATT CAATCTGCGT GGACTGCGGG GCCCCCAACC CCACGTGGGC CAGCTTGAAC 
CTGGGCGCCC TCATCTGCAT CGAGTGTTCT GGCATCCACC GCAACCTGGG CACACACCTG 
TCCCGCGTTC GCTCGCTGGA CTTGGACGAC TGGCCACGGG AGCTGACCCT GGTGCTGACG 
GCTATTGGCA ACGACACGGC CAACCGCGTG TGGGAAAGCG ACACGCGAGG CCGTGCCAAG 
CCCTCGCGGG ACTCTTCGCG GGAGGAGCGC GAGTCGTGGA TTCGCGCCAA GTACGAGCAG 
CTACTGTTCC TGGCGCCGCT GAGCACCTCG GAGGAGCCGC TGGGCCGCCA GCTGTGGGCC 
GCCGTGCAGG CCCAGGACGT GGCTACCGTT CTCCTGCTTT TGGCCCATGC GCGACACGGG 
CCGCTCGACA CCAGCGTAGA GGACCCACAG CTGCGCTCCC CACTCCACCT GGCGGCCGAG 
CTCGCCCACG TCGTCATCAC GCAACTGCTG CTGTGGTACG GCGCGGACGT GGCGGCCCGT 
GACGCCCAGG GCCGCACGGC GCTGTTCTAC GCCCGCCAGG CTGGAAGCCA GCTGTGCGCC 
GACATCCTTC TCCAGCACGG CTGCCCGGGT GAGGGCGGCA GCGCGGCCAC CACGCCCAGC 
GCGGCCACCA CGCCCAGCAT CACCGCCACG CCCAGCCCCC GCCGCCGGAG CAGCGCCGCT 
AGCGTGGGCC GCGCCGACGC CCCGGTTGCG CTGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874261.1
CDS	128..3706
Translation	MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAAGAAGARGSETGDPGSPRGAEEPGKKRHERLFHRQDALWISTSSAGTGGAEPPALSPAPASPARPVSPAPGRRLSLWAVPPGPPLSGGLSPDPKPGGAPTSSRRPLLSSPSWGGPEPEGRAGGGIPGSSSPHPGTGSRRLKVAPPPPAPKPCKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSSAGTGASVSAAATAAAAGGGGSTASTSGGVGAGAGARGKLSPRKGKSKTLDNSDLHPGPPAGSPPPLTLPPTPSPATAVTAASAQPPGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGSGPLPGPPSLSSGSGSRELLGAELRASPKAVINSQEWTLSRSIPELRLGVLGDARSGKSSLIHRFLTGSYQVLEKTESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDENSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCADMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGLEATTPMPSPSPSPSSLQPPPDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLTTPSKTEGSAGQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSICVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPSRDSSREERESWIRAKYEQLLFLAPLSTSEEPLGRQLWAAVQAQDVATVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSAATTPSAATTPSITATPSPRRRSSAASVGRADAPVALV

Target ORF information:

RefSeq Version	XM_017018772.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018772.1

ORF Insert Sequence:

ATGAGCCGGG GCGCGGGCGC GCTTCAGCGC CGGACAACGA CCTACCTCAT CTCGCTGACC 
CTGGTTAAGC TCGAGTCGGT GCCTCCGCCG CCGCCTTCTC CGTCTGCGGC CGCGGCCGGC 
GCCGCCGGTG CCAGAGGCTC CGAGACTGGG GATCCTGGCA GCCCCCGAGG CGCGGAGGAG 
CCGGGCAAGA AGCGGCACGA ACGTCTCTTC CACCGGCAGG ATGCGCTGTG GATCAGCACG 
AGCAGCGCGG GCACCGGGGG CGCGGAGCCC CCAGCCCTGT CCCCGGCTCC GGCCAGTCCG 
GCCCGCCCAG TCTCCCCCGC TCCCGGCCGC CGCCTCTCCC TCTGGGCCGT CCCTCCGGGA 
CCCCCGCTCT CCGGGGGACT GAGCCCCGAC CCCAAGCCTG GGGGCGCCCC CACCTCCTCC 
CGGCGCCCCC TGCTCAGCAG CCCGAGCTGG GGCGGCCCGG AGCCCGAAGG CCGGGCGGGC 
GGCGGCATCC CTGGCTCATC CTCTCCGCAC CCTGGCACCG GCAGCCGGAG GCTCAAGGTG 
GCGCCTCCTC CGCCGGCTCC CAAGCCTTGC AAGACCGTGA CCACGAGTGG AGCCAAAGCC 
GGCGGGGGCA AGGGCGCGGG TAGCCGCCTG TCATGGCCCG AAAGCGAGGG CAAGCCCAGG 
GTCAAGGGGT CAAAGAGCAG CGCCGGGACT GGAGCTTCGG TCTCTGCCGC CGCCACCGCC 
GCCGCCGCCG GGGGAGGGGG CTCTACAGCT TCGACCTCTG GTGGGGTCGG GGCTGGGGCT 
GGAGCCCGAG GGAAGTTGTC CCCTCGGAAA GGCAAGAGTA AGACCTTGGA CAACAGTGAC 
TTGCATCCGG GACCGCCTGC CGGCTCTCCT CCTCCGCTAA CCCTCCCACC AACTCCGAGT 
CCAGCCACTG CTGTCACCGC TGCTTCCGCG CAGCCCCCCG GGCCTGCACC TCCAATCACT 
CTGGAGCCTC CAGCTCCGGG GCTGAAACGG GGCCGGGAGG GGGGCCGAGC ATCCACTCGT 
GACCGCAAGA TGCTCAAGTT TATCAGCGGC ATCTTCACCA AGAGCACAGG AGGGCCTCCT 
GGCTCCGGGC CCCTTCCCGG ACCCCCCAGC CTGTCTTCTG GCAGCGGGTC CAGGGAGCTG 
CTGGGCGCCG AGCTCCGCGC TTCCCCTAAG GCTGTGATCA ATAGCCAGGA ATGGACTTTG 
AGCCGCTCCA TTCCTGAACT GCGCCTGGGT GTGCTGGGCG ATGCCAGGAG TGGGAAGTCA 
TCGCTCATCC ACCGATTCCT GACTGGCTCA TACCAGGTGC TGGAGAAGAC AGAGAGTGAG 
CAGTACAAGA AAGAAATGTT GGTGGATGGA CAGACACATC TGGTGCTAAT CCGAGAGGAA 
GCTGGGGCAC CTGATGCCAA GTTCTCAGGC TGGGCAGATG CTGTGATCTT CGTCTTCAGC 
CTGGAGGATG AGAACAGTTT CCAGGCTGTG AGCCGTCTCC ATGGGCAGCT GAGTTCCCTT 
CGCGGGGAGG GACGAGGAGG CCTGGCCTTG GCACTGGTGG GGACACAAGA CAGGATCAGT 
GCTTCCTCCC CTCGGGTGGT GGGAGATGCT CGTGCCAGAG CTCTGTGCGC GGACATGAAA 
CGCTGCAGCT ACTATGAGAC TTGTGCAACC TATGGGCTCA ATGTGGATCG GGTCTTCCAG 
GAGGTGGCCC AGAAGGTGGT GACCTTGCGC AAGCAGCAAC AGCTTCTGGC TGCCTGCAAG 
TCCCTGCCCA GCTCCCCAAG CCACTCAGCT GCATCCACTC CGGTAGCTGG CCAGGCTAGT 
AACGGGGGCC ACACTAGCGA CTACTCTTCT TCCCTCCCGT CCTCACCGAA TGTTGGTCAC 
CGGGAGCTCC GAGCCGAGGC AGCTGCAGTG GCTGGATTGA GCACCCCAGG GTCCCTGCAC 
CGGGCAGCCA AGCGCAGGAC CAGCCTTTTT GCGAATCGTC GGGGTAGTGA CTCCGAGAAA 
CGAAGCTTGG ATAGTCGGGG AGAGACAACA GGGAGTGGGC GAGCCATCCC CATCAAACAG 
AGCTTCCTAC TAAAACGAAG TGGCAATTCC TTGAACAAAG AATGGAAGAA GAAATATGTA 
ACCCTGTCCA GTAATGGCTT TCTACTCTAC CACCCCAGTA TTAACGATTA CATCCACAGT 
ACCCACGGCA AGGAGATGGA CTTGCTGCGA ACAACAGTCA AAGTCCCGGG CAAGCGGCCC 
CCGAGGGCCA TCTCTGCCTT TGGCCCCTCA GCCAGCATTA ACGGGCTCGT CAAGGACATG 
AGCACTGTCC AGATGGGTGA AGGCCTGGAA GCCACTACTC CCATGCCAAG CCCTAGCCCC 
AGCCCCAGTT CCCTGCAGCC ACCACCAGAT CAGACATCCA AACACCTGCT GAAGCCAGAC 
CGGAATTTGG CCCGAGCCCT CAGCACGGAC TGTACCCCAT CTGGAGACCT GAGCCCCCTG 
AGTCGGGAAC CCCCTCCTTC TCCCATGGTG AAGAAGCAGA GGAGGAAAAA ATTGACAACA 
CCATCCAAGA CTGAAGGCTC GGCTGGGCAG GCTGAAGCCA AGCGCAAAAT GTGGAAACTA 
AAATCCTTTG GTAGTTTAAG AAATATTTAT AAAGCAGAGG AAAACTTTGA GTTCCTGATC 
GTGTCCAGCA CGGGTCAGAC GTGGCACTTT GAGGCAGCCA GTTTTGAGGA GCGGGATGCC 
TGGGTCCAGG CCATCGAGAG TCAGATCCTA GCCAGTCTGC AATGCTGTGA GAGCAGCAAG 
GTCAAGCTGC GCACAGACAG CCAAAGCGAG GCCGTGGCCA TCCAGGCGAT CCGGAACGCC 
AAGGGGAATT CAATCTGCGT GGACTGCGGG GCCCCCAACC CCACGTGGGC CAGCTTGAAC 
CTGGGCGCCC TCATCTGCAT CGAGTGTTCT GGCATCCACC GCAACCTGGG CACACACCTG 
TCCCGCGTTC GCTCGCTGGA CTTGGACGAC TGGCCACGGG AGCTGACCCT GGTGCTGACG 
GCTATTGGCA ACGACACGGC CAACCGCGTG TGGGAAAGCG ACACGCGAGG CCGTGCCAAG 
CCCTCGCGGG ACTCTTCGCG GGAGGAGCGC GAGTCGTGGA TTCGCGCCAA GTACGAGCAG 
CTACTGTTCC TGGCGCCGCT GAGCACCTCG GAGGAGCCGC TGGGCCGCCA GCTGTGGGCC 
GCCGTGCAGG CCCAGGACGT GGCTACCGTT CTCCTGCTTT TGGCCCATGC GCGACACGGG 
CCGCTCGACA CCAGCGTAGA GGACCCACAG CTGCGCTCCC CACTCCACCT GGCGGCCGAG 
CTCGCCCACG TCGTCATCAC GCAACTGCTG CTGTGGTACG GCGCGGACGT GGCGGCCCGT 
GACGCCCAGG GCCGCACGGC GCTGTTCTAC GCCCGCCAGG CTGGAAGCCA GCTGTGCGCC 
GACATCCTTC TCCAGCACGG CTGCCCGGGT GAGGGCGGCA GCGCGGCCAC CACGCCCAGC 
GCGGCCACCA CGCCCAGCAT CACCGCCACG CCCAGCCCCC GCCGCCGGAG CAGCGCCGCT 
AGCGTGGGCC GCGCCGACGC CCCGGTTGCG CTGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGAP2 ( XM_017018772.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874261.1 Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant X1, mRNA.

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CloneID	OHu11929
Clone ID Related Accession (Same CDS sequence)	XM_017018772.1 , XM_017018772.2 , NM_001122772.2
Accession Version	XM_017018772.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018772.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGG GCGCGGGCGC GCTTCAGCGC CGGACAACGA CCTACCTCAT CTCGCTGACC 
CTGGTTAAGC TCGAGTCGGT GCCTCCGCCG CCGCCTTCTC CGTCTGCGGC CGCGGCCGGC 
GCCGCCGGTG CCAGAGGCTC CGAGACTGGG GATCCTGGCA GCCCCCGAGG CGCGGAGGAG 
CCGGGCAAGA AGCGGCACGA ACGTCTCTTC CACCGGCAGG ATGCGCTGTG GATCAGCACG 
AGCAGCGCGG GCACCGGGGG CGCGGAGCCC CCAGCCCTGT CCCCGGCTCC GGCCAGTCCG 
GCCCGCCCAG TCTCCCCCGC TCCCGGCCGC CGCCTCTCCC TCTGGGCCGT CCCTCCGGGA 
CCCCCGCTCT CCGGGGGACT GAGCCCCGAC CCCAAGCCTG GGGGCGCCCC CACCTCCTCC 
CGGCGCCCCC TGCTCAGCAG CCCGAGCTGG GGCGGCCCGG AGCCCGAAGG CCGGGCGGGC 
GGCGGCATCC CTGGCTCATC CTCTCCGCAC CCTGGCACCG GCAGCCGGAG GCTCAAGGTG 
GCGCCTCCTC CGCCGGCTCC CAAGCCTTGC AAGACCGTGA CCACGAGTGG AGCCAAAGCC 
GGCGGGGGCA AGGGCGCGGG TAGCCGCCTG TCATGGCCCG AAAGCGAGGG CAAGCCCAGG 
GTCAAGGGGT CAAAGAGCAG CGCCGGGACT GGAGCTTCGG TCTCTGCCGC CGCCACCGCC 
GCCGCCGCCG GGGGAGGGGG CTCTACAGCT TCGACCTCTG GTGGGGTCGG GGCTGGGGCT 
GGAGCCCGAG GGAAGTTGTC CCCTCGGAAA GGCAAGAGTA AGACCTTGGA CAACAGTGAC 
TTGCATCCGG GACCGCCTGC CGGCTCTCCT CCTCCGCTAA CCCTCCCACC AACTCCGAGT 
CCAGCCACTG CTGTCACCGC TGCTTCCGCG CAGCCCCCCG GGCCTGCACC TCCAATCACT 
CTGGAGCCTC CAGCTCCGGG GCTGAAACGG GGCCGGGAGG GGGGCCGAGC ATCCACTCGT 
GACCGCAAGA TGCTCAAGTT TATCAGCGGC ATCTTCACCA AGAGCACAGG AGGGCCTCCT 
GGCTCCGGGC CCCTTCCCGG ACCCCCCAGC CTGTCTTCTG GCAGCGGGTC CAGGGAGCTG 
CTGGGCGCCG AGCTCCGCGC TTCCCCTAAG GCTGTGATCA ATAGCCAGGA ATGGACTTTG 
AGCCGCTCCA TTCCTGAACT GCGCCTGGGT GTGCTGGGCG ATGCCAGGAG TGGGAAGTCA 
TCGCTCATCC ACCGATTCCT GACTGGCTCA TACCAGGTGC TGGAGAAGAC AGAGAGTGAG 
CAGTACAAGA AAGAAATGTT GGTGGATGGA CAGACACATC TGGTGCTAAT CCGAGAGGAA 
GCTGGGGCAC CTGATGCCAA GTTCTCAGGC TGGGCAGATG CTGTGATCTT CGTCTTCAGC 
CTGGAGGATG AGAACAGTTT CCAGGCTGTG AGCCGTCTCC ATGGGCAGCT GAGTTCCCTT 
CGCGGGGAGG GACGAGGAGG CCTGGCCTTG GCACTGGTGG GGACACAAGA CAGGATCAGT 
GCTTCCTCCC CTCGGGTGGT GGGAGATGCT CGTGCCAGAG CTCTGTGCGC GGACATGAAA 
CGCTGCAGCT ACTATGAGAC TTGTGCAACC TATGGGCTCA ATGTGGATCG GGTCTTCCAG 
GAGGTGGCCC AGAAGGTGGT GACCTTGCGC AAGCAGCAAC AGCTTCTGGC TGCCTGCAAG 
TCCCTGCCCA GCTCCCCAAG CCACTCAGCT GCATCCACTC CGGTAGCTGG CCAGGCTAGT 
AACGGGGGCC ACACTAGCGA CTACTCTTCT TCCCTCCCGT CCTCACCGAA TGTTGGTCAC 
CGGGAGCTCC GAGCCGAGGC AGCTGCAGTG GCTGGATTGA GCACCCCAGG GTCCCTGCAC 
CGGGCAGCCA AGCGCAGGAC CAGCCTTTTT GCGAATCGTC GGGGTAGTGA CTCCGAGAAA 
CGAAGCTTGG ATAGTCGGGG AGAGACAACA GGGAGTGGGC GAGCCATCCC CATCAAACAG 
AGCTTCCTAC TAAAACGAAG TGGCAATTCC TTGAACAAAG AATGGAAGAA GAAATATGTA 
ACCCTGTCCA GTAATGGCTT TCTACTCTAC CACCCCAGTA TTAACGATTA CATCCACAGT 
ACCCACGGCA AGGAGATGGA CTTGCTGCGA ACAACAGTCA AAGTCCCGGG CAAGCGGCCC 
CCGAGGGCCA TCTCTGCCTT TGGCCCCTCA GCCAGCATTA ACGGGCTCGT CAAGGACATG 
AGCACTGTCC AGATGGGTGA AGGCCTGGAA GCCACTACTC CCATGCCAAG CCCTAGCCCC 
AGCCCCAGTT CCCTGCAGCC ACCACCAGAT CAGACATCCA AACACCTGCT GAAGCCAGAC 
CGGAATTTGG CCCGAGCCCT CAGCACGGAC TGTACCCCAT CTGGAGACCT GAGCCCCCTG 
AGTCGGGAAC CCCCTCCTTC TCCCATGGTG AAGAAGCAGA GGAGGAAAAA ATTGACAACA 
CCATCCAAGA CTGAAGGCTC GGCTGGGCAG GCTGAAGCCA AGCGCAAAAT GTGGAAACTA 
AAATCCTTTG GTAGTTTAAG AAATATTTAT AAAGCAGAGG AAAACTTTGA GTTCCTGATC 
GTGTCCAGCA CGGGTCAGAC GTGGCACTTT GAGGCAGCCA GTTTTGAGGA GCGGGATGCC 
TGGGTCCAGG CCATCGAGAG TCAGATCCTA GCCAGTCTGC AATGCTGTGA GAGCAGCAAG 
GTCAAGCTGC GCACAGACAG CCAAAGCGAG GCCGTGGCCA TCCAGGCGAT CCGGAACGCC 
AAGGGGAATT CAATCTGCGT GGACTGCGGG GCCCCCAACC CCACGTGGGC CAGCTTGAAC 
CTGGGCGCCC TCATCTGCAT CGAGTGTTCT GGCATCCACC GCAACCTGGG CACACACCTG 
TCCCGCGTTC GCTCGCTGGA CTTGGACGAC TGGCCACGGG AGCTGACCCT GGTGCTGACG 
GCTATTGGCA ACGACACGGC CAACCGCGTG TGGGAAAGCG ACACGCGAGG CCGTGCCAAG 
CCCTCGCGGG ACTCTTCGCG GGAGGAGCGC GAGTCGTGGA TTCGCGCCAA GTACGAGCAG 
CTACTGTTCC TGGCGCCGCT GAGCACCTCG GAGGAGCCGC TGGGCCGCCA GCTGTGGGCC 
GCCGTGCAGG CCCAGGACGT GGCTACCGTT CTCCTGCTTT TGGCCCATGC GCGACACGGG 
CCGCTCGACA CCAGCGTAGA GGACCCACAG CTGCGCTCCC CACTCCACCT GGCGGCCGAG 
CTCGCCCACG TCGTCATCAC GCAACTGCTG CTGTGGTACG GCGCGGACGT GGCGGCCCGT 
GACGCCCAGG GCCGCACGGC GCTGTTCTAC GCCCGCCAGG CTGGAAGCCA GCTGTGCGCC 
GACATCCTTC TCCAGCACGG CTGCCCGGGT GAGGGCGGCA GCGCGGCCAC CACGCCCAGC 
GCGGCCACCA CGCCCAGCAT CACCGCCACG CCCAGCCCCC GCCGCCGGAG CAGCGCCGCT 
AGCGTGGGCC GCGCCGACGC CCCGGTTGCG CTGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874261.1
CDS	127..3705
Translation	MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAAGAAGARGSETGDPGSPRGAEEPGKKRHERLFHRQDALWISTSSAGTGGAEPPALSPAPASPARPVSPAPGRRLSLWAVPPGPPLSGGLSPDPKPGGAPTSSRRPLLSSPSWGGPEPEGRAGGGIPGSSSPHPGTGSRRLKVAPPPPAPKPCKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSSAGTGASVSAAATAAAAGGGGSTASTSGGVGAGAGARGKLSPRKGKSKTLDNSDLHPGPPAGSPPPLTLPPTPSPATAVTAASAQPPGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGSGPLPGPPSLSSGSGSRELLGAELRASPKAVINSQEWTLSRSIPELRLGVLGDARSGKSSLIHRFLTGSYQVLEKTESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDENSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCADMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGLEATTPMPSPSPSPSSLQPPPDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLTTPSKTEGSAGQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSICVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPSRDSSREERESWIRAKYEQLLFLAPLSTSEEPLGRQLWAAVQAQDVATVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSAATTPSAATTPSITATPSPRRRSSAASVGRADAPVALV

Target ORF information:

RefSeq Version	XM_017018772.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018772.2

ORF Insert Sequence:

ATGAGCCGGG GCGCGGGCGC GCTTCAGCGC CGGACAACGA CCTACCTCAT CTCGCTGACC 
CTGGTTAAGC TCGAGTCGGT GCCTCCGCCG CCGCCTTCTC CGTCTGCGGC CGCGGCCGGC 
GCCGCCGGTG CCAGAGGCTC CGAGACTGGG GATCCTGGCA GCCCCCGAGG CGCGGAGGAG 
CCGGGCAAGA AGCGGCACGA ACGTCTCTTC CACCGGCAGG ATGCGCTGTG GATCAGCACG 
AGCAGCGCGG GCACCGGGGG CGCGGAGCCC CCAGCCCTGT CCCCGGCTCC GGCCAGTCCG 
GCCCGCCCAG TCTCCCCCGC TCCCGGCCGC CGCCTCTCCC TCTGGGCCGT CCCTCCGGGA 
CCCCCGCTCT CCGGGGGACT GAGCCCCGAC CCCAAGCCTG GGGGCGCCCC CACCTCCTCC 
CGGCGCCCCC TGCTCAGCAG CCCGAGCTGG GGCGGCCCGG AGCCCGAAGG CCGGGCGGGC 
GGCGGCATCC CTGGCTCATC CTCTCCGCAC CCTGGCACCG GCAGCCGGAG GCTCAAGGTG 
GCGCCTCCTC CGCCGGCTCC CAAGCCTTGC AAGACCGTGA CCACGAGTGG AGCCAAAGCC 
GGCGGGGGCA AGGGCGCGGG TAGCCGCCTG TCATGGCCCG AAAGCGAGGG CAAGCCCAGG 
GTCAAGGGGT CAAAGAGCAG CGCCGGGACT GGAGCTTCGG TCTCTGCCGC CGCCACCGCC 
GCCGCCGCCG GGGGAGGGGG CTCTACAGCT TCGACCTCTG GTGGGGTCGG GGCTGGGGCT 
GGAGCCCGAG GGAAGTTGTC CCCTCGGAAA GGCAAGAGTA AGACCTTGGA CAACAGTGAC 
TTGCATCCGG GACCGCCTGC CGGCTCTCCT CCTCCGCTAA CCCTCCCACC AACTCCGAGT 
CCAGCCACTG CTGTCACCGC TGCTTCCGCG CAGCCCCCCG GGCCTGCACC TCCAATCACT 
CTGGAGCCTC CAGCTCCGGG GCTGAAACGG GGCCGGGAGG GGGGCCGAGC ATCCACTCGT 
GACCGCAAGA TGCTCAAGTT TATCAGCGGC ATCTTCACCA AGAGCACAGG AGGGCCTCCT 
GGCTCCGGGC CCCTTCCCGG ACCCCCCAGC CTGTCTTCTG GCAGCGGGTC CAGGGAGCTG 
CTGGGCGCCG AGCTCCGCGC TTCCCCTAAG GCTGTGATCA ATAGCCAGGA ATGGACTTTG 
AGCCGCTCCA TTCCTGAACT GCGCCTGGGT GTGCTGGGCG ATGCCAGGAG TGGGAAGTCA 
TCGCTCATCC ACCGATTCCT GACTGGCTCA TACCAGGTGC TGGAGAAGAC AGAGAGTGAG 
CAGTACAAGA AAGAAATGTT GGTGGATGGA CAGACACATC TGGTGCTAAT CCGAGAGGAA 
GCTGGGGCAC CTGATGCCAA GTTCTCAGGC TGGGCAGATG CTGTGATCTT CGTCTTCAGC 
CTGGAGGATG AGAACAGTTT CCAGGCTGTG AGCCGTCTCC ATGGGCAGCT GAGTTCCCTT 
CGCGGGGAGG GACGAGGAGG CCTGGCCTTG GCACTGGTGG GGACACAAGA CAGGATCAGT 
GCTTCCTCCC CTCGGGTGGT GGGAGATGCT CGTGCCAGAG CTCTGTGCGC GGACATGAAA 
CGCTGCAGCT ACTATGAGAC TTGTGCAACC TATGGGCTCA ATGTGGATCG GGTCTTCCAG 
GAGGTGGCCC AGAAGGTGGT GACCTTGCGC AAGCAGCAAC AGCTTCTGGC TGCCTGCAAG 
TCCCTGCCCA GCTCCCCAAG CCACTCAGCT GCATCCACTC CGGTAGCTGG CCAGGCTAGT 
AACGGGGGCC ACACTAGCGA CTACTCTTCT TCCCTCCCGT CCTCACCGAA TGTTGGTCAC 
CGGGAGCTCC GAGCCGAGGC AGCTGCAGTG GCTGGATTGA GCACCCCAGG GTCCCTGCAC 
CGGGCAGCCA AGCGCAGGAC CAGCCTTTTT GCGAATCGTC GGGGTAGTGA CTCCGAGAAA 
CGAAGCTTGG ATAGTCGGGG AGAGACAACA GGGAGTGGGC GAGCCATCCC CATCAAACAG 
AGCTTCCTAC TAAAACGAAG TGGCAATTCC TTGAACAAAG AATGGAAGAA GAAATATGTA 
ACCCTGTCCA GTAATGGCTT TCTACTCTAC CACCCCAGTA TTAACGATTA CATCCACAGT 
ACCCACGGCA AGGAGATGGA CTTGCTGCGA ACAACAGTCA AAGTCCCGGG CAAGCGGCCC 
CCGAGGGCCA TCTCTGCCTT TGGCCCCTCA GCCAGCATTA ACGGGCTCGT CAAGGACATG 
AGCACTGTCC AGATGGGTGA AGGCCTGGAA GCCACTACTC CCATGCCAAG CCCTAGCCCC 
AGCCCCAGTT CCCTGCAGCC ACCACCAGAT CAGACATCCA AACACCTGCT GAAGCCAGAC 
CGGAATTTGG CCCGAGCCCT CAGCACGGAC TGTACCCCAT CTGGAGACCT GAGCCCCCTG 
AGTCGGGAAC CCCCTCCTTC TCCCATGGTG AAGAAGCAGA GGAGGAAAAA ATTGACAACA 
CCATCCAAGA CTGAAGGCTC GGCTGGGCAG GCTGAAGCCA AGCGCAAAAT GTGGAAACTA 
AAATCCTTTG GTAGTTTAAG AAATATTTAT AAAGCAGAGG AAAACTTTGA GTTCCTGATC 
GTGTCCAGCA CGGGTCAGAC GTGGCACTTT GAGGCAGCCA GTTTTGAGGA GCGGGATGCC 
TGGGTCCAGG CCATCGAGAG TCAGATCCTA GCCAGTCTGC AATGCTGTGA GAGCAGCAAG 
GTCAAGCTGC GCACAGACAG CCAAAGCGAG GCCGTGGCCA TCCAGGCGAT CCGGAACGCC 
AAGGGGAATT CAATCTGCGT GGACTGCGGG GCCCCCAACC CCACGTGGGC CAGCTTGAAC 
CTGGGCGCCC TCATCTGCAT CGAGTGTTCT GGCATCCACC GCAACCTGGG CACACACCTG 
TCCCGCGTTC GCTCGCTGGA CTTGGACGAC TGGCCACGGG AGCTGACCCT GGTGCTGACG 
GCTATTGGCA ACGACACGGC CAACCGCGTG TGGGAAAGCG ACACGCGAGG CCGTGCCAAG 
CCCTCGCGGG ACTCTTCGCG GGAGGAGCGC GAGTCGTGGA TTCGCGCCAA GTACGAGCAG 
CTACTGTTCC TGGCGCCGCT GAGCACCTCG GAGGAGCCGC TGGGCCGCCA GCTGTGGGCC 
GCCGTGCAGG CCCAGGACGT GGCTACCGTT CTCCTGCTTT TGGCCCATGC GCGACACGGG 
CCGCTCGACA CCAGCGTAGA GGACCCACAG CTGCGCTCCC CACTCCACCT GGCGGCCGAG 
CTCGCCCACG TCGTCATCAC GCAACTGCTG CTGTGGTACG GCGCGGACGT GGCGGCCCGT 
GACGCCCAGG GCCGCACGGC GCTGTTCTAC GCCCGCCAGG CTGGAAGCCA GCTGTGCGCC 
GACATCCTTC TCCAGCACGG CTGCCCGGGT GAGGGCGGCA GCGCGGCCAC CACGCCCAGC 
GCGGCCACCA CGCCCAGCAT CACCGCCACG CCCAGCCCCC GCCGCCGGAG CAGCGCCGCT 
AGCGTGGGCC GCGCCGACGC CCCGGTTGCG CTGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGAP2 ( XM_005268625.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268682.1 Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant X2, mRNA.

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CloneID	OHu46299
Clone ID Related Accession (Same CDS sequence)	XM_005268625.3
Accession Version	XM_005268625.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268625.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGG GCGCGGGCGC GCTTCAGCGC CGGACAACGA CCTACCTCAT CTCGCTGACC 
CTGGTTAAGC TCGAGTCGGT GCCTCCGCCG CCGCCTTCTC CGTCTGCGGC CGCGGCCGGC 
GCCGCCGGTG CCAGAGGCTC CGAGACTGGG GATCCTGGCA GCCCCCGAGG CGCGGAGGAG 
CCGGGCAAGA AGCGGCACGA ACGTCTCTTC CACCGGCAGG ATGCGCTGTG GATCAGCACG 
AGCAGCGCGG GCACCGGGGG CGCGGAGCCC CCAGCCCTGT CCCCGGCTCC GGCCAGTCCG 
GCCCGCCCAG TCTCCCCCGC TCCCGGCCGC CGCCTCTCCC TCTGGGCCGT CCCTCCGGGA 
CCCCCGCTCT CCGGGGGACT GAGCCCCGAC CCCAAGCCTG GGGGCGCCCC CACCTCCTCC 
CGGCGCCCCC TGCTCAGCAG CCCGAGCTGG GGCGGCCCGG AGCCCGAAGG CCGGGCGGGC 
GGCGGCATCC CTGGCTCATC CTCTCCGCAC CCTGGCACCG GCAGCCGGAG GCTCAAGGTG 
GCGCCTCCTC CGCCGGCTCC CAAGCCTTGC AAGACCGTGA CCACGAGTGG AGCCAAAGCC 
GGCGGGGGCA AGGGCGCGGG TAGCCGCCTG TCATGGCCCG AAAGCGAGGG CAAGCCCAGG 
GTCAAGGGGT CAAAGAGCAG CGCCGGGACT GGAGCTTCGG TCTCTGCCGC CGCCACCGCC 
GCCGCCGCCG GGGGAGGGGG CTCTACAGCT TCGACCTCTG GTGGGGTCGG GGCTGGGGCT 
GGAGCCCGAG GGAAGTTGTC CCCTCGGAAA GGCAAGAGTA AGACCTTGGA CAACAGTGAC 
TTGCATCCGG GACCGCCTGC CGGCTCTCCT CCTCCGCTAA CCCTCCCACC AACTCCGAGT 
CCAGCCACTG CTGTCACCGC TGCTTCCGCG CAGCCCCCCG GGCCTGCACC TCCAATCACT 
CTGGAGCCTC CAGCTCCGGG GCTGAAACGG GGCCGGGAGG GGGGCCGAGC ATCCACTCGT 
GACCGCAAGA TGCTCAAGTT TATCAGCGGC ATCTTCACCA AGAGCACAGG AGGGCCTCCT 
GGCTCCGGGC CCCTTCCCGG ACCCCCCAGC CTGTCTTCTG GCAGCGGGTC CAGGGAGCTG 
CTGGGCGCCG AGCTCCGCGC TTCCCCTAAG GCTGTGATCA ATAGCCAGGA ATGGACTTTG 
AGCCGCTCCA TTCCTGAACT GCGCCTGGGT GTGCTGGGCG ATGCCAGGAG TGGGAAGTCA 
TCGCTCATCC ACCGATTCCT GACTGGCTCA TACCAGGTGC TGGAGAAGAC AGAGAGTGAG 
CAGTACAAGA AAGAAATGTT GGTGGATGGA CAGACACATC TGGTGCTAAT CCGAGAGGAA 
GCTGGGGCAC CTGATGCCAA GTTCTCAGGC TGGGCAGATG CTGTGATCTT CGTCTTCAGC 
CTGGAGGATG AGAACAGTTT CCAGGCTGTG AGCCGTCTCC ATGGGCAGCT GAGTTCCCTT 
CGCGGGGAGG GACGAGGAGG CCTGGCCTTG GCACTGGTGG GGACACAAGA CAGGATCAGT 
GCTTCCTCCC CTCGGGTGGT GGGAGATGCT CGTGCCAGAG CTCTGTGCGC GGACATGAAA 
CGCTGCAGCT ACTATGAGAC TTGTGCAACC TATGGGCTCA ATGTGGATCG GGTCTTCCAG 
GAGGTGGCCC AGAAGGTGGT GACCTTGCGC AAGCAGCAAC AGCTTCTGGC TGCCTGCAAG 
TCCCTGCCCA GCTCCCCAAG CCACTCAGCT GCATCCACTC CGGTAGCTGG CCAGGCTAGT 
AACGGGGGCC ACACTAGCGA CTACTCTTCT TCCCTCCCGT CCTCACCGAA TGTTGGTCAC 
CGGGAGCTCC GAGCCGAGGC AGCTGCAGTG GCTGGATTGA GCACCCCAGG GTCCCTGCAC 
CGGGCAGCCA AGCGCAGGAC CAGCCTTTTT GCGAATCGTC GGGGTAGTGA CTCCGAGAAA 
CGAAGCTTGG ATAGTCGGGG AGAGACAACA GGGAGTGGGC GAGCCATCCC CATCAAACAG 
AGCTTCCTAC TAAAACGAAG TGGCAATTCC TTGAACAAAG AATGGAAGAA GAAATATGTA 
ACCCTGTCCA GTAATGGCTT TCTACTCTAC CACCCCAGTA TTAACGATTA CATCCACAGT 
ACCCACGGCA AGGAGATGGA CTTGCTGCGA ACAACAGTCA AAGTCCCGGG CAAGCGGCCC 
CCGAGGGCCA TCTCTGCCTT TGGCCCCTCA GCCAGCATTA ACGGGCTCGT CAAGGACATG 
AGCACTGTCC AGATGGGTGA AGGCCTGGAA GCCACTACTC CCATGCCAAG CCCTAGCCCC 
AGCCCCAGTT CCCTGCAGCC ACCACCAGAT CAGACATCCA AACACCTGCT GAAGCCAGAC 
CGGAATTTGG CCCGAGCCCT CAGCACGGAC TGTACCCCAT CTGGAGACCT GAGCCCCCTG 
AGTCGGGAAC CCCCTCCTTC TCCCATGGTG AAGAAGCAGA GGAGGAAAAA ATTGACAACA 
CCATCCAAGA CTGAAGGCTC GGCTGGGCAG GCTGAAGAGG AAAACTTTGA GTTCCTGATC 
GTGTCCAGCA CGGGTCAGAC GTGGCACTTT GAGGCAGCCA GTTTTGAGGA GCGGGATGCC 
TGGGTCCAGG CCATCGAGAG TCAGATCCTA GCCAGTCTGC AATGCTGTGA GAGCAGCAAG 
GTCAAGCTGC GCACAGACAG CCAAAGCGAG GCCGTGGCCA TCCAGGCGAT CCGGAACGCC 
AAGGGGAATT CAATCTGCGT GGACTGCGGG GCCCCCAACC CCACGTGGGC CAGCTTGAAC 
CTGGGCGCCC TCATCTGCAT CGAGTGTTCT GGCATCCACC GCAACCTGGG CACACACCTG 
TCCCGCGTTC GCTCGCTGGA CTTGGACGAC TGGCCACGGG AGCTGACCCT GGTGCTGACG 
GCTATTGGCA ACGACACGGC CAACCGCGTG TGGGAAAGCG ACACGCGAGG CCGTGCCAAG 
CCCTCGCGGG ACTCTTCGCG GGAGGAGCGC GAGTCGTGGA TTCGCGCCAA GTACGAGCAG 
CTACTGTTCC TGGCGCCGCT GAGCACCTCG GAGGAGCCGC TGGGCCGCCA GCTGTGGGCC 
GCCGTGCAGG CCCAGGACGT GGCTACCGTT CTCCTGCTTT TGGCCCATGC GCGACACGGG 
CCGCTCGACA CCAGCGTAGA GGACCCACAG CTGCGCTCCC CACTCCACCT GGCGGCCGAG 
CTCGCCCACG TCGTCATCAC GCAACTGCTG CTGTGGTACG GCGCGGACGT GGCGGCCCGT 
GACGCCCAGG GCCGCACGGC GCTGTTCTAC GCCCGCCAGG CTGGAAGCCA GCTGTGCGCC 
GACATCCTTC TCCAGCACGG CTGCCCGGGT GAGGGCGGCA GCGCGGCCAC CACGCCCAGC 
GCGGCCACCA CGCCCAGCAT CACCGCCACG CCCAGCCCCC GCCGCCGGAG CAGCGCCGCT 
AGCGTGGGCC GCGCCGACGC CCCGGTTGCG CTGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268682.1
CDS	127..3645
Translation	MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAAGAAGARGSETGDPGSPRGAEEPGKKRHERLFHRQDALWISTSSAGTGGAEPPALSPAPASPARPVSPAPGRRLSLWAVPPGPPLSGGLSPDPKPGGAPTSSRRPLLSSPSWGGPEPEGRAGGGIPGSSSPHPGTGSRRLKVAPPPPAPKPCKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSSAGTGASVSAAATAAAAGGGGSTASTSGGVGAGAGARGKLSPRKGKSKTLDNSDLHPGPPAGSPPPLTLPPTPSPATAVTAASAQPPGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGSGPLPGPPSLSSGSGSRELLGAELRASPKAVINSQEWTLSRSIPELRLGVLGDARSGKSSLIHRFLTGSYQVLEKTESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDENSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCADMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGLEATTPMPSPSPSPSSLQPPPDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLTTPSKTEGSAGQAEEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSICVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPSRDSSREERESWIRAKYEQLLFLAPLSTSEEPLGRQLWAAVQAQDVATVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSAATTPSAATTPSITATPSPRRRSSAASVGRADAPVALV

Target ORF information:

RefSeq Version	XM_005268625.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268625.3

ORF Insert Sequence:

ATGAGCCGGG GCGCGGGCGC GCTTCAGCGC CGGACAACGA CCTACCTCAT CTCGCTGACC 
CTGGTTAAGC TCGAGTCGGT GCCTCCGCCG CCGCCTTCTC CGTCTGCGGC CGCGGCCGGC 
GCCGCCGGTG CCAGAGGCTC CGAGACTGGG GATCCTGGCA GCCCCCGAGG CGCGGAGGAG 
CCGGGCAAGA AGCGGCACGA ACGTCTCTTC CACCGGCAGG ATGCGCTGTG GATCAGCACG 
AGCAGCGCGG GCACCGGGGG CGCGGAGCCC CCAGCCCTGT CCCCGGCTCC GGCCAGTCCG 
GCCCGCCCAG TCTCCCCCGC TCCCGGCCGC CGCCTCTCCC TCTGGGCCGT CCCTCCGGGA 
CCCCCGCTCT CCGGGGGACT GAGCCCCGAC CCCAAGCCTG GGGGCGCCCC CACCTCCTCC 
CGGCGCCCCC TGCTCAGCAG CCCGAGCTGG GGCGGCCCGG AGCCCGAAGG CCGGGCGGGC 
GGCGGCATCC CTGGCTCATC CTCTCCGCAC CCTGGCACCG GCAGCCGGAG GCTCAAGGTG 
GCGCCTCCTC CGCCGGCTCC CAAGCCTTGC AAGACCGTGA CCACGAGTGG AGCCAAAGCC 
GGCGGGGGCA AGGGCGCGGG TAGCCGCCTG TCATGGCCCG AAAGCGAGGG CAAGCCCAGG 
GTCAAGGGGT CAAAGAGCAG CGCCGGGACT GGAGCTTCGG TCTCTGCCGC CGCCACCGCC 
GCCGCCGCCG GGGGAGGGGG CTCTACAGCT TCGACCTCTG GTGGGGTCGG GGCTGGGGCT 
GGAGCCCGAG GGAAGTTGTC CCCTCGGAAA GGCAAGAGTA AGACCTTGGA CAACAGTGAC 
TTGCATCCGG GACCGCCTGC CGGCTCTCCT CCTCCGCTAA CCCTCCCACC AACTCCGAGT 
CCAGCCACTG CTGTCACCGC TGCTTCCGCG CAGCCCCCCG GGCCTGCACC TCCAATCACT 
CTGGAGCCTC CAGCTCCGGG GCTGAAACGG GGCCGGGAGG GGGGCCGAGC ATCCACTCGT 
GACCGCAAGA TGCTCAAGTT TATCAGCGGC ATCTTCACCA AGAGCACAGG AGGGCCTCCT 
GGCTCCGGGC CCCTTCCCGG ACCCCCCAGC CTGTCTTCTG GCAGCGGGTC CAGGGAGCTG 
CTGGGCGCCG AGCTCCGCGC TTCCCCTAAG GCTGTGATCA ATAGCCAGGA ATGGACTTTG 
AGCCGCTCCA TTCCTGAACT GCGCCTGGGT GTGCTGGGCG ATGCCAGGAG TGGGAAGTCA 
TCGCTCATCC ACCGATTCCT GACTGGCTCA TACCAGGTGC TGGAGAAGAC AGAGAGTGAG 
CAGTACAAGA AAGAAATGTT GGTGGATGGA CAGACACATC TGGTGCTAAT CCGAGAGGAA 
GCTGGGGCAC CTGATGCCAA GTTCTCAGGC TGGGCAGATG CTGTGATCTT CGTCTTCAGC 
CTGGAGGATG AGAACAGTTT CCAGGCTGTG AGCCGTCTCC ATGGGCAGCT GAGTTCCCTT 
CGCGGGGAGG GACGAGGAGG CCTGGCCTTG GCACTGGTGG GGACACAAGA CAGGATCAGT 
GCTTCCTCCC CTCGGGTGGT GGGAGATGCT CGTGCCAGAG CTCTGTGCGC GGACATGAAA 
CGCTGCAGCT ACTATGAGAC TTGTGCAACC TATGGGCTCA ATGTGGATCG GGTCTTCCAG 
GAGGTGGCCC AGAAGGTGGT GACCTTGCGC AAGCAGCAAC AGCTTCTGGC TGCCTGCAAG 
TCCCTGCCCA GCTCCCCAAG CCACTCAGCT GCATCCACTC CGGTAGCTGG CCAGGCTAGT 
AACGGGGGCC ACACTAGCGA CTACTCTTCT TCCCTCCCGT CCTCACCGAA TGTTGGTCAC 
CGGGAGCTCC GAGCCGAGGC AGCTGCAGTG GCTGGATTGA GCACCCCAGG GTCCCTGCAC 
CGGGCAGCCA AGCGCAGGAC CAGCCTTTTT GCGAATCGTC GGGGTAGTGA CTCCGAGAAA 
CGAAGCTTGG ATAGTCGGGG AGAGACAACA GGGAGTGGGC GAGCCATCCC CATCAAACAG 
AGCTTCCTAC TAAAACGAAG TGGCAATTCC TTGAACAAAG AATGGAAGAA GAAATATGTA 
ACCCTGTCCA GTAATGGCTT TCTACTCTAC CACCCCAGTA TTAACGATTA CATCCACAGT 
ACCCACGGCA AGGAGATGGA CTTGCTGCGA ACAACAGTCA AAGTCCCGGG CAAGCGGCCC 
CCGAGGGCCA TCTCTGCCTT TGGCCCCTCA GCCAGCATTA ACGGGCTCGT CAAGGACATG 
AGCACTGTCC AGATGGGTGA AGGCCTGGAA GCCACTACTC CCATGCCAAG CCCTAGCCCC 
AGCCCCAGTT CCCTGCAGCC ACCACCAGAT CAGACATCCA AACACCTGCT GAAGCCAGAC 
CGGAATTTGG CCCGAGCCCT CAGCACGGAC TGTACCCCAT CTGGAGACCT GAGCCCCCTG 
AGTCGGGAAC CCCCTCCTTC TCCCATGGTG AAGAAGCAGA GGAGGAAAAA ATTGACAACA 
CCATCCAAGA CTGAAGGCTC GGCTGGGCAG GCTGAAGAGG AAAACTTTGA GTTCCTGATC 
GTGTCCAGCA CGGGTCAGAC GTGGCACTTT GAGGCAGCCA GTTTTGAGGA GCGGGATGCC 
TGGGTCCAGG CCATCGAGAG TCAGATCCTA GCCAGTCTGC AATGCTGTGA GAGCAGCAAG 
GTCAAGCTGC GCACAGACAG CCAAAGCGAG GCCGTGGCCA TCCAGGCGAT CCGGAACGCC 
AAGGGGAATT CAATCTGCGT GGACTGCGGG GCCCCCAACC CCACGTGGGC CAGCTTGAAC 
CTGGGCGCCC TCATCTGCAT CGAGTGTTCT GGCATCCACC GCAACCTGGG CACACACCTG 
TCCCGCGTTC GCTCGCTGGA CTTGGACGAC TGGCCACGGG AGCTGACCCT GGTGCTGACG 
GCTATTGGCA ACGACACGGC CAACCGCGTG TGGGAAAGCG ACACGCGAGG CCGTGCCAAG 
CCCTCGCGGG ACTCTTCGCG GGAGGAGCGC GAGTCGTGGA TTCGCGCCAA GTACGAGCAG 
CTACTGTTCC TGGCGCCGCT GAGCACCTCG GAGGAGCCGC TGGGCCGCCA GCTGTGGGCC 
GCCGTGCAGG CCCAGGACGT GGCTACCGTT CTCCTGCTTT TGGCCCATGC GCGACACGGG 
CCGCTCGACA CCAGCGTAGA GGACCCACAG CTGCGCTCCC CACTCCACCT GGCGGCCGAG 
CTCGCCCACG TCGTCATCAC GCAACTGCTG CTGTGGTACG GCGCGGACGT GGCGGCCCGT 
GACGCCCAGG GCCGCACGGC GCTGTTCTAC GCCCGCCAGG CTGGAAGCCA GCTGTGCGCC 
GACATCCTTC TCCAGCACGG CTGCCCGGGT GAGGGCGGCA GCGCGGCCAC CACGCCCAGC 
GCGGCCACCA CGCCCAGCAT CACCGCCACG CCCAGCCCCC GCCGCCGGAG CAGCGCCGCT 
AGCGTGGGCC GCGCCGACGC CCCGGTTGCG CTGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGAP2 ( XM_005268626.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268683.1 Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu46300
Clone ID Related Accession (Same CDS sequence)	XM_005268626.2
Accession Version	XM_005268626.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268626.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGCCC AGAGGCAGTT CGTTGTAGCT GCAGTGAGAG CAGAAGTCAG ACGACATGAG 
GTGGCCAAGC AGGCTCTAAA CCGCCTCAGG AAGCTGGCAG AGAGGGTGGA CGACCCCGAA 
CTCCAGGACA GCATCCAGGC CTCATTGGAC AGCATTCGAG AGGCTGTGAT CAATAGCCAG 
GAATGGACTT TGAGCCGCTC CATTCCTGAA CTGCGCCTGG GTGTGCTGGG CGATGCCAGG 
AGTGGGAAGT CATCGCTCAT CCACCGATTC CTGACTGGCT CATACCAGGT GCTGGAGAAG 
ACAGAGAGTG AGCAGTACAA GAAAGAAATG TTGGTGGATG GACAGACACA TCTGGTGCTA 
ATCCGAGAGG AAGCTGGGGC ACCTGATGCC AAGTTCTCAG GCTGGGCAGA TGCTGTGATC 
TTCGTCTTCA GCCTGGAGGA TGAGAACAGT TTCCAGGCTG TGAGCCGTCT CCATGGGCAG 
CTGAGTTCCC TTCGCGGGGA GGGACGAGGA GGCCTGGCCT TGGCACTGGT GGGGACACAA 
GACAGGATCA GTGCTTCCTC CCCTCGGGTG GTGGGAGATG CTCGTGCCAG AGCTCTGTGC 
GCGGACATGA AACGCTGCAG CTACTATGAG ACTTGTGCAA CCTATGGGCT CAATGTGGAT 
CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG GTGACCTTGC GCAAGCAGCA ACAGCTTCTG 
GCTGCCTGCA AGTCCCTGCC CAGCTCCCCA AGCCACTCAG CTGCATCCAC TCCGGTAGCT 
GGCCAGGCTA GTAACGGGGG CCACACTAGC GACTACTCTT CTTCCCTCCC GTCCTCACCG 
AATGTTGGTC ACCGGGAGCT CCGAGCCGAG GCAGCTGCAG TGGCTGGATT GAGCACCCCA 
GGGTCCCTGC ACCGGGCAGC CAAGCGCAGG ACCAGCCTTT TTGCGAATCG TCGGGGTAGT 
GACTCCGAGA AACGAAGCTT GGATAGTCGG GGAGAGACAA CAGGGAGTGG GCGAGCCATC 
CCCATCAAAC AGAGCTTCCT ACTAAAACGA AGTGGCAATT CCTTGAACAA AGAATGGAAG 
AAGAAATATG TAACCCTGTC CAGTAATGGC TTTCTACTCT ACCACCCCAG TATTAACGAT 
TACATCCACA GTACCCACGG CAAGGAGATG GACTTGCTGC GAACAACAGT CAAAGTCCCG 
GGCAAGCGGC CCCCGAGGGC CATCTCTGCC TTTGGCCCCT CAGCCAGCAT TAACGGGCTC 
GTCAAGGACA TGAGCACTGT CCAGATGGGT GAAGGCCTGG AAGCCACTAC TCCCATGCCA 
AGCCCTAGCC CCAGCCCCAG TTCCCTGCAG CCACCACCAG ATCAGACATC CAAACACCTG 
CTGAAGCCAG ACCGGAATTT GGCCCGAGCC CTCAGCACGG ACTGTACCCC ATCTGGAGAC 
CTGAGCCCCC TGAGTCGGGA ACCCCCTCCT TCTCCCATGG TGAAGAAGCA GAGGAGGAAA 
AAATTGACAA CACCATCCAA GACTGAAGGC TCGGCTGGGC AGGCTGAAGC CAAGCGCAAA 
ATGTGGAAAC TAAAATCCTT TGGTAGTTTA AGAAATATTT ATAAAGCAGA GGAAAACTTT 
GAGTTCCTGA TCGTGTCCAG CACGGGTCAG ACGTGGCACT TTGAGGCAGC CAGTTTTGAG 
GAGCGGGATG CCTGGGTCCA GGCCATCGAG AGTCAGATCC TAGCCAGTCT GCAATGCTGT 
GAGAGCAGCA AGGTCAAGCT GCGCACAGAC AGCCAAAGCG AGGCCGTGGC CATCCAGGCG 
ATCCGGAACG CCAAGGGGAA TTCAATCTGC GTGGACTGCG GGGCCCCCAA CCCCACGTGG 
GCCAGCTTGA ACCTGGGCGC CCTCATCTGC ATCGAGTGTT CTGGCATCCA CCGCAACCTG 
GGCACACACC TGTCCCGCGT TCGCTCGCTG GACTTGGACG ACTGGCCACG GGAGCTGACC 
CTGGTGCTGA CGGCTATTGG CAACGACACG GCCAACCGCG TGTGGGAAAG CGACACGCGA 
GGCCGTGCCA AGCCCTCGCG GGACTCTTCG CGGGAGGAGC GCGAGTCGTG GATTCGCGCC 
AAGTACGAGC AGCTACTGTT CCTGGCGCCG CTGAGCACCT CGGAGGAGCC GCTGGGCCGC 
CAGCTGTGGG CCGCCGTGCA GGCCCAGGAC GTGGCTACCG TTCTCCTGCT TTTGGCCCAT 
GCGCGACACG GGCCGCTCGA CACCAGCGTA GAGGACCCAC AGCTGCGCTC CCCACTCCAC 
CTGGCGGCCG AGCTCGCCCA CGTCGTCATC ACGCAACTGC TGCTGTGGTA CGGCGCGGAC 
GTGGCGGCCC GTGACGCCCA GGGCCGCACG GCGCTGTTCT ACGCCCGCCA GGCTGGAAGC 
CAGCTGTGCG CCGACATCCT TCTCCAGCAC GGCTGCCCGG GTGAGGGCGG CAGCGCGGCC 
ACCACGCCCA GCGCGGCCAC CACGCCCAGC ATCACCGCCA CGCCCAGCCC CCGCCGCCGG 
AGCAGCGCCG CTAGCGTGGG CCGCGCCGAC GCCCCGGTTG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268683.1
CDS	131..2701
Translation	MHAQRQFVVAAVRAEVRRHEVAKQALNRLRKLAERVDDPELQDSIQASLDSIREAVINSQEWTLSRSIPELRLGVLGDARSGKSSLIHRFLTGSYQVLEKTESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDENSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCADMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGLEATTPMPSPSPSPSSLQPPPDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLTTPSKTEGSAGQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSICVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPSRDSSREERESWIRAKYEQLLFLAPLSTSEEPLGRQLWAAVQAQDVATVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSAATTPSAATTPSITATPSPRRRSSAASVGRADAPVALV

Target ORF information:

RefSeq Version	XM_005268626.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268626.2

ORF Insert Sequence:

ATGCATGCCC AGAGGCAGTT CGTTGTAGCT GCAGTGAGAG CAGAAGTCAG ACGACATGAG 
GTGGCCAAGC AGGCTCTAAA CCGCCTCAGG AAGCTGGCAG AGAGGGTGGA CGACCCCGAA 
CTCCAGGACA GCATCCAGGC CTCATTGGAC AGCATTCGAG AGGCTGTGAT CAATAGCCAG 
GAATGGACTT TGAGCCGCTC CATTCCTGAA CTGCGCCTGG GTGTGCTGGG CGATGCCAGG 
AGTGGGAAGT CATCGCTCAT CCACCGATTC CTGACTGGCT CATACCAGGT GCTGGAGAAG 
ACAGAGAGTG AGCAGTACAA GAAAGAAATG TTGGTGGATG GACAGACACA TCTGGTGCTA 
ATCCGAGAGG AAGCTGGGGC ACCTGATGCC AAGTTCTCAG GCTGGGCAGA TGCTGTGATC 
TTCGTCTTCA GCCTGGAGGA TGAGAACAGT TTCCAGGCTG TGAGCCGTCT CCATGGGCAG 
CTGAGTTCCC TTCGCGGGGA GGGACGAGGA GGCCTGGCCT TGGCACTGGT GGGGACACAA 
GACAGGATCA GTGCTTCCTC CCCTCGGGTG GTGGGAGATG CTCGTGCCAG AGCTCTGTGC 
GCGGACATGA AACGCTGCAG CTACTATGAG ACTTGTGCAA CCTATGGGCT CAATGTGGAT 
CGGGTCTTCC AGGAGGTGGC CCAGAAGGTG GTGACCTTGC GCAAGCAGCA ACAGCTTCTG 
GCTGCCTGCA AGTCCCTGCC CAGCTCCCCA AGCCACTCAG CTGCATCCAC TCCGGTAGCT 
GGCCAGGCTA GTAACGGGGG CCACACTAGC GACTACTCTT CTTCCCTCCC GTCCTCACCG 
AATGTTGGTC ACCGGGAGCT CCGAGCCGAG GCAGCTGCAG TGGCTGGATT GAGCACCCCA 
GGGTCCCTGC ACCGGGCAGC CAAGCGCAGG ACCAGCCTTT TTGCGAATCG TCGGGGTAGT 
GACTCCGAGA AACGAAGCTT GGATAGTCGG GGAGAGACAA CAGGGAGTGG GCGAGCCATC 
CCCATCAAAC AGAGCTTCCT ACTAAAACGA AGTGGCAATT CCTTGAACAA AGAATGGAAG 
AAGAAATATG TAACCCTGTC CAGTAATGGC TTTCTACTCT ACCACCCCAG TATTAACGAT 
TACATCCACA GTACCCACGG CAAGGAGATG GACTTGCTGC GAACAACAGT CAAAGTCCCG 
GGCAAGCGGC CCCCGAGGGC CATCTCTGCC TTTGGCCCCT CAGCCAGCAT TAACGGGCTC 
GTCAAGGACA TGAGCACTGT CCAGATGGGT GAAGGCCTGG AAGCCACTAC TCCCATGCCA 
AGCCCTAGCC CCAGCCCCAG TTCCCTGCAG CCACCACCAG ATCAGACATC CAAACACCTG 
CTGAAGCCAG ACCGGAATTT GGCCCGAGCC CTCAGCACGG ACTGTACCCC ATCTGGAGAC 
CTGAGCCCCC TGAGTCGGGA ACCCCCTCCT TCTCCCATGG TGAAGAAGCA GAGGAGGAAA 
AAATTGACAA CACCATCCAA GACTGAAGGC TCGGCTGGGC AGGCTGAAGC CAAGCGCAAA 
ATGTGGAAAC TAAAATCCTT TGGTAGTTTA AGAAATATTT ATAAAGCAGA GGAAAACTTT 
GAGTTCCTGA TCGTGTCCAG CACGGGTCAG ACGTGGCACT TTGAGGCAGC CAGTTTTGAG 
GAGCGGGATG CCTGGGTCCA GGCCATCGAG AGTCAGATCC TAGCCAGTCT GCAATGCTGT 
GAGAGCAGCA AGGTCAAGCT GCGCACAGAC AGCCAAAGCG AGGCCGTGGC CATCCAGGCG 
ATCCGGAACG CCAAGGGGAA TTCAATCTGC GTGGACTGCG GGGCCCCCAA CCCCACGTGG 
GCCAGCTTGA ACCTGGGCGC CCTCATCTGC ATCGAGTGTT CTGGCATCCA CCGCAACCTG 
GGCACACACC TGTCCCGCGT TCGCTCGCTG GACTTGGACG ACTGGCCACG GGAGCTGACC 
CTGGTGCTGA CGGCTATTGG CAACGACACG GCCAACCGCG TGTGGGAAAG CGACACGCGA 
GGCCGTGCCA AGCCCTCGCG GGACTCTTCG CGGGAGGAGC GCGAGTCGTG GATTCGCGCC 
AAGTACGAGC AGCTACTGTT CCTGGCGCCG CTGAGCACCT CGGAGGAGCC GCTGGGCCGC 
CAGCTGTGGG CCGCCGTGCA GGCCCAGGAC GTGGCTACCG TTCTCCTGCT TTTGGCCCAT 
GCGCGACACG GGCCGCTCGA CACCAGCGTA GAGGACCCAC AGCTGCGCTC CCCACTCCAC 
CTGGCGGCCG AGCTCGCCCA CGTCGTCATC ACGCAACTGC TGCTGTGGTA CGGCGCGGAC 
GTGGCGGCCC GTGACGCCCA GGGCCGCACG GCGCTGTTCT ACGCCCGCCA GGCTGGAAGC 
CAGCTGTGCG CCGACATCCT TCTCCAGCAC GGCTGCCCGG GTGAGGGCGG CAGCGCGGCC 
ACCACGCCCA GCGCGGCCAC CACGCCCAGC ATCACCGCCA CGCCCAGCCC CCGCCGCCGG 
AGCAGCGCCG CTAGCGTGGG CCGCGCCGAC GCCCCGGTTG CGCTGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Co-amplification of phosphoinositide 3-kinase enhancer A and cyclin-dependent kinase 4 triggers glioblastoma progression.
Oncogene36(32)4562-4572(2017 Aug)
Qi Q,Kang SS,Zhang S,Pham C,Fu H,Brat DJ,Ye K

?-Synuclein binds and sequesters PIKE-L into Lewy bodies, triggering dopaminergic cell death via AMPK hyperactivation.
Proceedings of the National Academy of Sciences of the United States of America114(5)1183-1188(2017 Jan)
Kang SS,Zhang Z,Liu X,Manfredsson FP,He L,Iuvone PM,Cao X,Sun YE,Jin L,Ye K

Phosphoinositide-3-Kinase Enhancers, PIKEs: Their Biological Functions and Roles in Cancer.
Anticancer research36(3)1103-9(2016 Mar)
Jia W,Feng YI,Sanders AJ,Davies EL,Jiang WG

Reduced expression of E-cadherin and p120-catenin and elevated expression of PLC-?1 and PIKE are associated with aggressiveness of oral squamous cell carcinoma.
International journal of clinical and experimental pathology8(8)9042-51(2015)
Jiang Y,Liao L,Shrestha C,Ji S,Chen Y,Peng J,Wang L,Liao E,Xie Z

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AGAP2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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