ARAP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARAP1
Entrez Gene ID	116985
Full Name	ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Synonyms	CENTD2
General protein information	Preferred Names ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 Names arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containing protein 1 centaurin-delta-2 cnt-d2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.4
Summary	The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008].
Disorder MIM:	606646

mRNA and Protein(s)

mRNA	Protein	Name
NM_015242.4	NP_056057.2	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform a
NM_001040118.2	NP_001035207.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform c
NM_001135190.1	NP_001128662.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform d
NM_001369489.1	NP_001356418.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform e

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARAP1	XP_508625.3
Canis lupus familiaris (dog)	ARAP1	XP_005633606.1
Danio rerio (zebrafish)	LOC100332526	XP_002667258.3
Bos taurus (cattle)	ARAP1	XP_002693483.1
Mus musculus (house mouse)	Arap1	NP_001035200.1
Rattus norvegicus (Norway rat)	Arap1	XP_006229959.1
Homo sapiens (human)	ARAP1	NP_001035207.1
	ARAP1	XP_001114962.1

Gene Ontology
Bibliography

Related articles in PubMed

Decreased STARD10 Expression Is Associated with Defective Insulin Secretion in Humans and Mice.
Carrat GR, Hu M, Nguyen-Tu MS, Chabosseau P, Gaulton KJ, van de Bunt M, Siddiq A, Falchi M, Thurner M, Canouil M, Pattou F, Leclerc I, Pullen TJ, Cane MC, Prabhala P, Greenwald W, Schulte A, Marchetti P, Ibberson M, MacDonald PE, Manning Fox JE, Gloyn AL, Froguel P, Solimena M, McCarthy MI, Rutter GA
American journal of human genetics100(2)238-256(2017 Feb)

Joint effect of CENTD2 and KCNQ1 polymorphisms on the risk of type 2 diabetes mellitus among Chinese Han population.
Qian Y, Dong M, Lu F, Li H, Jin G, Hu Z, Shen C, Shen H
Molecular and cellular endocrinology40746-51(2015 May)

Genome-wide trans-ancestry meta-analysis provides insight into the genetic architecture of type 2 diabetes susceptibility.
, , , , , Mahajan A, Go MJ, Zhang W, Below JE, Gaulton KJ, Ferreira T, Horikoshi M, Johnson AD, Ng MC, Prokopenko I, Saleheen D, Wang X, Zeggini E, Abecasis GR, Adair LS, Almgren P, Atalay M, Aung T, Baldassarre D, Balkau B, Bao Y, Barnett AH, Barroso I, Basit A, Been LF, Beilby J, Bell GI, Benediktsson R, Bergman RN, Boehm BO, Boerwinkle E, Bonnycastle LL, Burtt N, Cai Q, Campbell H, Carey J, Cauchi S, Caulfield M, Chan JC, Chang LC, Chang TJ, Chang YC, Charpentier G, Chen CH, Chen H, Chen YT, Chia KS, Chidambaram M, Chines PS, Cho NH, Cho YM, Chuang LM, Collins FS, Cornelis MC, Couper DJ, Crenshaw AT, van Dam RM, Danesh J, Das D, de Faire U, Dedoussis G, Deloukas P, Dimas AS, Dina C, Doney AS, Donnelly PJ, Dorkhan M, van Duijn C, Dupuis J, Edkins S, Elliott P, Emilsson V, Erbel R, Eriksson JG, Escobedo J, Esko T, Eury E, Florez JC, Fontanillas P, Forouhi NG, Forsen T, Fox C, Fraser RM, Frayling TM, Froguel P, Frossard P, Gao Y, Gertow K, Gieger C, Gigante B, Grallert H, Grant GB, Grrop LC, Groves CJ, Grundberg E, Guiducci C, Hamsten A, Han BG, Hara K, Hassanali N, Hattersley AT, Hayward C, Hedman AK, Herder C, Hofman A, Holmen OL, Hovingh K, Hreidarsson AB, Hu C, Hu FB, Hui J, Humphries SE, Hunt SE, Hunter DJ, Hveem K, Hydrie ZI, Ikegami H, Illig T, Ingelsson E, Islam M, Isomaa B, Jackson AU, Jafar T, James A, Jia W, J?ckel KH, Jonsson A, Jowett JB, Kadowaki T, Kang HM, Kanoni S, Kao WH, Kathiresan S, Kato N, Katulanda P, Keinanen-Kiukaanniemi KM, Kelly AM, Khan H, Khaw KT, Khor CC, Kim HL, Kim S, Kim YJ, Kinnunen L, Klopp N, Kong A, Korpi-Hy?v?lti E, Kowlessur S, Kraft P, Kravic J, Kristensen MM, Krithika S, Kumar A, Kumate J, Kuusisto J, Kwak SH, Laakso M, Lagou V, Lakka TA, Langenberg C, Langford C, Lawrence R, Leander K, Lee JM, Lee NR, Li M, Li X, Li Y, Liang J, Liju S, Lim WY, Lind L, Lindgren CM, Lindholm E, Liu CT, Liu JJ, Lobbens S, Long J, Loos RJ, Lu W, Luan J, Lyssenko V, Ma RC, Maeda S, M?gi R, M?nnisto S, Matthews DR, Meigs JB, Melander O, Metspalu A, Meyer J, Mirza G, Mihailov E, Moebus S, Mohan V, Mohlke KL, Morris AD, M?hleisen TW, M?ller-Nurasyid M, Musk B, Nakamura J, Nakashima E, Navarro P, Ng PK, Nica AC, Nilsson PM, Nj?lstad I, N?then MM, Ohnaka K, Ong TH, Owen KR, Palmer CN, Pankow JS, Park KS, Parkin M, Pechlivanis S, Pedersen NL, Peltonen L, Perry JR, Peters A, Pinidiyapathirage JM, Platou CG, Potter S, Price JF, Qi L, Radha V, Rallidis L, Rasheed A, Rathman W, Rauramaa R, Raychaudhuri S, Rayner NW, Rees SD, Rehnberg E, Ripatti S, Robertson N, Roden M, Rossin EJ, Rudan I, Rybin D, Saaristo TE, Salomaa V, Saltevo J, Samuel M, Sanghera DK, Saramies J, Scott J, Scott LJ, Scott RA, Segr? AV, Sehmi J, Sennblad B, Shah N, Shah S, Shera AS, Shu XO, Shuldiner AR, Sigur?sson G, Sijbrands E, Silveira A, Sim X, Sivapalaratnam S, Small KS, So WY, Stan??kov? A, Stefansson K, Steinbach G, Steinthorsdottir V, Stirrups K, Strawbridge RJ, Stringham HM, Sun Q, Suo C, Syv?nen AC, Takayanagi R, Takeuchi F, Tay WT, Teslovich TM, Thorand B, Thorleifsson G, Thorsteinsdottir U, Tikkanen E, Trakalo J, Tremoli E, Trip MD, Tsai FJ, Tuomi T, Tuomilehto J, Uitterlinden AG, Valladares-Salgado A, Vedantam S, Veglia F, Voight BF, Wang C, Wareham NJ, Wennauer R, Wickremasinghe AR, Wilsgaard T, Wilson JF, Wiltshire S, Winckler W, Wong TY, Wood AR, Wu JY, Wu Y, Yamamoto K, Yamauchi T, Yang M, Yengo L, Yokota M, Young R, Zabaneh D, Zhang F, Zhang R, Zheng W, Zimmet PZ, Altshuler D, Bowden DW, Cho YS, Cox NJ, Cruz M, Hanis CL, Kooner J, Lee JY, Seielstad M, Teo YY, Boehnke M, Parra EJ, Chambers JC, Tai ES, McCarthy MI, Morris AP
Nature genetics46(3)234-44(2014 Mar)

A common functional regulatory variant at a type 2 diabetes locus upregulates ARAP1 expression in the pancreatic beta cell.
Kulzer JR, Stitzel ML, Morken MA, Huyghe JR, Fuchsberger C, Kuusisto J, Laakso M, Boehnke M, Collins FS, Mohlke KL
American journal of human genetics94(2)186-97(2014 Feb)

A genome-wide approach accounting for body mass index identifies genetic variants influencing fasting glycemic traits and insulin resistance.
Manning AK, Hivert MF, Scott RA, Grimsby JL, Bouatia-Naji N, Chen H, Rybin D, Liu CT, Bielak LF, Prokopenko I, Amin N, Barnes D, Cadby G, Hottenga JJ, Ingelsson E, Jackson AU, Johnson T, Kanoni S, Ladenvall C, Lagou V, Lahti J, Lecoeur C, Liu Y, Martinez-Larrad MT, Montasser ME, Navarro P, Perry JR, Rasmussen-Torvik LJ, Salo P, Sattar N, Shungin D, Strawbridge RJ, Tanaka T, van Duijn CM, An P, de Andrade M, Andrews JS, Aspelund T, Atalay M, Aulchenko Y, Balkau B, Bandinelli S, Beckmann JS, Beilby JP, Bellis C, Bergman RN, Blangero J, Boban M, Boehnke M, Boerwinkle E, Bonnycastle LL, Boomsma DI, Borecki IB, B?ttcher Y, Bouchard C, Brunner E, Budimir D, Campbell H, Carlson O, Chines PS, Clarke R, Collins FS, Corbat?n-Anchuelo A, Couper D, de Faire U, Dedoussis GV, Deloukas P, Dimitriou M, Egan JM, Eiriksdottir G, Erdos MR, Eriksson JG, Eury E, Ferrucci L, Ford I, Forouhi NG, Fox CS, Franzosi MG, Franks PW, Frayling TM, Froguel P, Galan P, de Geus E, Gigante B, Glazer NL, Goel A, Groop L, Gudnason V, Hallmans G, Hamsten A, Hansson O, Harris TB, Hayward C, Heath S, Hercberg S, Hicks AA, Hingorani A, Hofman A, Hui J, Hung J, Jarvelin MR, Jhun MA, Johnson PC, Jukema JW, Jula A, Kao WH, Kaprio J, Kardia SL, Keinanen-Kiukaanniemi S, Kivimaki M, Kolcic I, Kovacs P, Kumari M, Kuusisto J, Kyvik KO, Laakso M, Lakka T, Lannfelt L, Lathrop GM, Launer LJ, Leander K, Li G, Lind L, Lindstrom J, Lobbens S, Loos RJ, Luan J, Lyssenko V, M?gi R, Magnusson PK, Marmot M, Meneton P, Mohlke KL, Mooser V, Morken MA, Miljkovic I, Narisu N, O'Connell J, Ong KK, Oostra BA, Palmer LJ, Palotie A, Pankow JS, Peden JF, Pedersen NL, Pehlic M, Peltonen L, Penninx B, Pericic M, Perola M, Perusse L, Peyser PA, Polasek O, Pramstaller PP, Province MA, R?ikk?nen K, Rauramaa R, Rehnberg E, Rice K, Rotter JI, Rudan I, Ruokonen A, Saaristo T, Sabater-Lleal M, Salomaa V, Savage DB, Saxena R, Schwarz P, Seedorf U, Sennblad B, Serrano-Rios M, Shuldiner AR, Sijbrands EJ, Siscovick DS, Smit JH, Small KS, Smith NL, Smith AV, Stan??kov? A, Stirrups K, Stumvoll M, Sun YV, Swift AJ, T?njes A, Tuomilehto J, Trompet S, Uitterlinden AG, Uusitupa M, Vikstr?m M, Vitart V, Vohl MC, Voight BF, Vollenweider P, Waeber G, Waterworth DM, Watkins H, Wheeler E, Widen E, Wild SH, Willems SM, Willemsen G, Wilson JF, Witteman JC, Wright AF, Yaghootkar H, Zelenika D, Zemunik T, Zgaga L, , , Wareham NJ, McCarthy MI, Barroso I, Watanabe RM, Florez JC, Dupuis J, Meigs JB, Langenberg C
Nature genetics44(6)659-69(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Quantitative trait loci genes analysis reveals association between T2D risk variants at the ARAP1/STARD10 locus and STARD10 but not ARAP1 expression.
Title: Decreased STARD10 Expression Is Associated with Defective Insulin Secretion in Humans and Mice.

Genetic variant rs1552224 of CENTD2 on chromosome 11 contributes to an independent effect as well as joint cumulative effect with rs2237897 of KCNQ1 on the risk of type 2 diabetes mellitus in Chinese Han population.
Title: Joint effect of CENTD2 and KCNQ1 polymorphisms on the risk of type 2 diabetes mellitus among Chinese Han population.

The proinsulin-decreasing and T2D-risk Alleles are associated with increased ARAP1 mRNA levels in primary human pancreatic islets.
Title: A common functional regulatory variant at a type 2 diabetes locus upregulates ARAP1 expression in the pancreatic beta cell.

a novel molecular mechanism of circular dorsal ruffles ring size control through the ARAP1-Arf1/5 pathway.
Title: ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5.

Arap1 is highly expressed in the renal vasculature, and its expression is suppressed by angiotensin II.
Title: Angiotensin AT1 receptor-associated protein Arap1 in the kidney vasculature is suppressed by angiotensin II.

The following ARAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu108006	NM_001369489.1 Latest version!	Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu15146	NM_015242.4 Latest version!	Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$853.30 $1219.00
OHu15209	NM_001135190.1 Latest version!	Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu14897	NM_001040118.2 Latest version!	Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARAP1 ( NM_001369489.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356418.1 Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu108006
Clone ID Related Accession (Same CDS sequence)	NM_001369489.1
Accession Version	NM_001369489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP003065.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144562.1, SRR7346977.1844968.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGACCAAGA AGGAGGAGCC CCCACCGAGC CGAGTCCCAC GGGCCGTGCG CGTGGCCAGT 
CTGCTGAGCG AGGGAGAGGA ACTGTCTGGG GACGACCAAG GGGATGAGGA AGAGGATGAC 
CACGCCTATG AGGGCGTCCC CAATGGCGGA TGGCATACCA GCAGCCTGAG CTTGTCCTTG 
CCCAGCACAA TAGCTGCGCC ACACCCCATG GACGGGCCGC CTGGGGGCTC CACCCCCGTC 
ACACCAGTCA TCAAGGCTGG CTGGCTGGAC AAGAACCCAC CGCAGGGATC TTACATCTAT 
CAGAAACGAT GGGTGAGACT GGATACTGAT CACCTGCGAT ACTTTGACAG TAACAAGGAC 
GCTTACTCTA AGCGCTTTAT CTCTGTGGCC TGCATCTCCC ACGTGGCTGC CATCGGGGAC 
CAGAAGTTTG AAGTGATCAC AAACAACCGA ACCTTTGCCT TCCGGGCAGA GAGTGATGTG 
GAGCGGAAGG AGTGGATGCA GGCCCTGCAG CAGGCCATGG CTGAGCAGCG TGCCCGGGCC 
CGGCTCTCTA GCGCTTATCT GCTGGGAGTT CCAGGCTCAG AGCAGCCTGA CCGCGCTGGC 
AGCCTGGAGC TTCGTGGCTT CAAGAATAAG CTGTACGTGG CCGTGGTCGG GGACAAAGTG 
CAGCTCTACA AGAATCTAGA GGAGTACCAC CTGGGCATTG GCATCACCTT CATCGACATG 
AGCGTGGGCA ACGTGAAGGA AGTGGACCGG CGCAGCTTCG ACCTCACCAC GCCCTACCGC 
ATCTTCAGCT TCTCTGCTGA CTCAGAGCTA GAGAAGGAGC AGTGGTTGGA GGCCATGCAG 
GGAGCCATCG CTGAGGCCCT GTCTACCTCG GAGGTGGCCG AGCGCATCTG GGCTGCAGCC 
CCCAACAGGT TCTGTGCTGA CTGCGGGGCT CCTCAGCCTG ACTGGGCCTC CATCAACCTC 
TGTGTTGTTA TCTGCAAGCG CTGTGCAGGG GAGCACCGTG GCCTGGGCGC TGGCGTCTCC 
AAGGTGCGGA GCCTGAAGAT GGACAGGAAG GTGTGGACAG AAACACTTAT CGAGCTCTTC 
TTACAGCTGG GGAATGGCGC TGGGAACCGC TTCTGGGCAG CCAACGTGCC CCCCAGTGAG 
GCCCTGCAGC CCAGCAGCAG CCCCAGCACC CGGCGGTGCC ACCTGGAGGC CAAGTACCGT 
GAGGGCAAGT ACCGCCGCTA CCACCCGCTC TTTGGCAACC AGGAGGAGCT GGACAAGGCC 
CTGTGTGCTG CAGTCACCAC CACAGACCTG GCTGAGACCC AGGCGCTCCT GGGCTGTGGG 
GCTGGGATCA ACTGCTTCTC GGGGGACCCT GAGGCCCCCA CGCCCCTGGC TCTTGCAGAG 
CAGGCGGGGC AGACGCTGCA GATGGAATTC CTTCGGAACA ACCGGACCAC AGAGGTGCCT 
CGGCTGGACT CGATGAAGCC CCTGGAAAAG CACTACTCAG TTGTCCTGCC GACCGTGAGC 
CACAGTGGCT TCCTCTACAA GACTGCCTCT GCCGGCAAGC TGCTACAGGA CCGCCGGGCC 
CGGGAAGAGT TCAGCCGGCG CTGGTGTGTC CTTGGTGACG GGGTCCTGAG CTACTTTGAG 
AATGAGCGGG CAGTGACCCC CAATGGAGAG ATTCGGGCCA GCGAGATTGT GTGCCTGGCA 
GTGCCCCCTC CTGACACCCA TGGCTTTGAG CACACCTTTG AGGTGTACAC GGAGGGAGAA 
CGGCTGTACC TGTTTGGGCT GGAGAGTGCG GAGCAGGCTC ATGAGTGGGT CAAGTGTATT 
GCTAAGGCAT TCGTGCCTCC CCTAGCCGAG GATCTGCTGG CCCGGGATTT TGAGCGGCTG 
GGACGCCTAC CCTACAAAGC TGGCCTGAGC CTACAGCGGG CCCAGGAGGG CTGGTTCTCT 
CTCAGTGGCT CGGAGCTCCG TGCTGTCTTC CCGGAGGGGC CCTGCGAAGA GCCGCTGCAA 
CTACGGAAAC TGCAGGAGCT TTCCATCCAG GGGGACAGTG AGAACCAGGT GCTGGTGCTG 
GTGGAGCGAA GGAGGACACT GTACATACAG GGCGAGCGGC GGCTGGACTT CATGGGTTGG 
CTGGGGGCCA TCCAGAAAGC AGCCGCCAGC ATGGGGGACA CGCTGTCGGA GCAGCAGCTT 
GGGGACTCGG ATATCCCGGT GATCGTGTAC CGCTGTGTGG ACTACATCAC GCAGTGCGGC 
CTGACCTCCG AGGGCATCTA CCGCAAGTGT GGGCAGACAT CGAAGACACA GCGGCTGCTG 
GAGAGCCTGC GGCAGGATGC GCGCTCTGTG CACCTCAAGG AGGGCGAGCA GCACGTGGAT 
GATGTTTCCT CGGCGCTCAA GCGCTTCCTG CGCGACCTGC CTGATGGGCT CTTCACTCGC 
GCCCAGCGCC TAACCTGGCT GGAGGCCTCA GAGATTGAGG ACGAGGAGGA GAAGGTCTCC 
AGGTACCGAG AGCTGCTGGT GCGGCTGCCC CCTGTCAACC GGGCCACAGT GAAGGCCCTT 
ATCAGCCACC TGTACTGTGT TCAGTGCTTC TCAGACACGA ACCAGATGAA CGTGCACAAC 
CTGGCAATTG TGTTTGGGCC CACGCTCTTC CAGACAGATG GGCAGGACTA CAAGGCTGGC 
CGTGTGGTGG AAGACCTCAT TAACCACTAT GTGGTGGTGT TTAGTGTGGA TGAGGAAGAG 
CTCAGGAAGC AGCGGGAGGA GATCACTGCC ATTGTGAAGA TGCGCGTGGC TGGCACTGCC 
AGTGGGACCC AGCATGCCGG TGACTTCATC TGCACAGTGT ATCTGGAAGA GAAGAAGGCA 
GAGACTGAGC AGCATATCAA GGTCCCAGCA TCCATGACTG CTGAGGAGCT CACCCTGGAG 
ATCCTGGATC GCCGGAACGT GGGCATCAGG GAGAAGGACT ATTGGACCTG CTTTGAGGTC 
AACGAGAGGG AGGAGGCAGA GCGCCCCCTG CACTTTGCGG AGAAGGTGCT GCCCATCCTG 
CACGGGCTGG GCACGGACAG CCACCTGGTG GTGAAGAAGC ACCAGGCCAT GGAGGCCATG 
CTGCTGTACC TGGCCAGCCG TGTCGGTGAC ACCAAGCATG GCATGATGAA GTTCCGTGAG 
GACCGCAGCC TCCTGGGCCT GGGCCTGCCC TCAGGTGGCT TCCACGATCG CTACTTCATC 
CTCAACAGCA GCTGCTTGCG GCTCTACAAG GAGGTCCGGA GTCACCGGCC TGAGAAGGAG 
TGGCCTATTA AGAGTCTCAA AGTCTACCTG GGAGTGAAGA AGAAACTCAG GCCACCCACC 
TGCTGGGGCT TCACAGTGGT GCATGAGACA GAGAAACATG AGAAGCAGCA GTGGTACCTC 
TGCTGTGACA CACAGATGGA GCTCCGGGAG TGGTTCGCTA CCTTTCTGTT TGTGCAGCAT 
GACGGCCTGG TGTGGCCCTC AGAGCCCTCA CGCGTGTCCC GGGCAGTGCC TGAGGTCCGG 
CTGGGTAGTG TGTCACTGAT CCCCCTTCGA GGTAGTGAAA ATGAAATGCG CCGGAGTGTG 
GCTGCCTTCA CCGCGGACCC TCTGTCTCTT CTGCGCAACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356418.1
CDS	718..4302
Translation	MTKKEEPPPSRVPRAVRVASLLSEGEELSGDDQGDEEEDDHAYEGVPNGGWHTSSLSLSLPSTIAAPHPMDGPPGGSTPVTPVIKAGWLDKNPPQGSYIYQKRWVRLDTDHLRYFDSNKDAYSKRFISVACISHVAAIGDQKFEVITNNRTFAFRAESDVERKEWMQALQQAMAEQRARARLSSAYLLGVPGSEQPDRAGSLELRGFKNKLYVAVVGDKVQLYKNLEEYHLGIGITFIDMSVGNVKEVDRRSFDLTTPYRIFSFSADSELEKEQWLEAMQGAIAEALSTSEVAERIWAAAPNRFCADCGAPQPDWASINLCVVICKRCAGEHRGLGAGVSKVRSLKMDRKVWTETLIELFLQLGNGAGNRFWAANVPPSEALQPSSSPSTRRCHLEAKYREGKYRRYHPLFGNQEELDKALCAAVTTTDLAETQALLGCGAGINCFSGDPEAPTPLALAEQAGQTLQMEFLRNNRTTEVPRLDSMKPLEKHYSVVLPTVSHSGFLYKTASAGKLLQDRRAREEFSRRWCVLGDGVLSYFENERAVTPNGEIRASEIVCLAVPPPDTHGFEHTFEVYTEGERLYLFGLESAEQAHEWVKCIAKAFVPPLAEDLLARDFERLGRLPYKAGLSLQRAQEGWFSLSGSELRAVFPEGPCEEPLQLRKLQELSIQGDSENQVLVLVERRRTLYIQGERRLDFMGWLGAIQKAAASMGDTLSEQQLGDSDIPVIVYRCVDYITQCGLTSEGIYRKCGQTSKTQRLLESLRQDARSVHLKEGEQHVDDVSSALKRFLRDLPDGLFTRAQRLTWLEASEIEDEEEKVSRYRELLVRLPPVNRATVKALISHLYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFSVDEEELRKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIREKDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRVSRAVPEVRLGSVSLIPLRGSENEMRRSVAAFTADPLSLLRNV

Target ORF information:

RefSeq Version	NM_001369489.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369489.1

ORF Insert Sequence:

ATGACCAAGA AGGAGGAGCC CCCACCGAGC CGAGTCCCAC GGGCCGTGCG CGTGGCCAGT 
CTGCTGAGCG AGGGAGAGGA ACTGTCTGGG GACGACCAAG GGGATGAGGA AGAGGATGAC 
CACGCCTATG AGGGCGTCCC CAATGGCGGA TGGCATACCA GCAGCCTGAG CTTGTCCTTG 
CCCAGCACAA TAGCTGCGCC ACACCCCATG GACGGGCCGC CTGGGGGCTC CACCCCCGTC 
ACACCAGTCA TCAAGGCTGG CTGGCTGGAC AAGAACCCAC CGCAGGGATC TTACATCTAT 
CAGAAACGAT GGGTGAGACT GGATACTGAT CACCTGCGAT ACTTTGACAG TAACAAGGAC 
GCTTACTCTA AGCGCTTTAT CTCTGTGGCC TGCATCTCCC ACGTGGCTGC CATCGGGGAC 
CAGAAGTTTG AAGTGATCAC AAACAACCGA ACCTTTGCCT TCCGGGCAGA GAGTGATGTG 
GAGCGGAAGG AGTGGATGCA GGCCCTGCAG CAGGCCATGG CTGAGCAGCG TGCCCGGGCC 
CGGCTCTCTA GCGCTTATCT GCTGGGAGTT CCAGGCTCAG AGCAGCCTGA CCGCGCTGGC 
AGCCTGGAGC TTCGTGGCTT CAAGAATAAG CTGTACGTGG CCGTGGTCGG GGACAAAGTG 
CAGCTCTACA AGAATCTAGA GGAGTACCAC CTGGGCATTG GCATCACCTT CATCGACATG 
AGCGTGGGCA ACGTGAAGGA AGTGGACCGG CGCAGCTTCG ACCTCACCAC GCCCTACCGC 
ATCTTCAGCT TCTCTGCTGA CTCAGAGCTA GAGAAGGAGC AGTGGTTGGA GGCCATGCAG 
GGAGCCATCG CTGAGGCCCT GTCTACCTCG GAGGTGGCCG AGCGCATCTG GGCTGCAGCC 
CCCAACAGGT TCTGTGCTGA CTGCGGGGCT CCTCAGCCTG ACTGGGCCTC CATCAACCTC 
TGTGTTGTTA TCTGCAAGCG CTGTGCAGGG GAGCACCGTG GCCTGGGCGC TGGCGTCTCC 
AAGGTGCGGA GCCTGAAGAT GGACAGGAAG GTGTGGACAG AAACACTTAT CGAGCTCTTC 
TTACAGCTGG GGAATGGCGC TGGGAACCGC TTCTGGGCAG CCAACGTGCC CCCCAGTGAG 
GCCCTGCAGC CCAGCAGCAG CCCCAGCACC CGGCGGTGCC ACCTGGAGGC CAAGTACCGT 
GAGGGCAAGT ACCGCCGCTA CCACCCGCTC TTTGGCAACC AGGAGGAGCT GGACAAGGCC 
CTGTGTGCTG CAGTCACCAC CACAGACCTG GCTGAGACCC AGGCGCTCCT GGGCTGTGGG 
GCTGGGATCA ACTGCTTCTC GGGGGACCCT GAGGCCCCCA CGCCCCTGGC TCTTGCAGAG 
CAGGCGGGGC AGACGCTGCA GATGGAATTC CTTCGGAACA ACCGGACCAC AGAGGTGCCT 
CGGCTGGACT CGATGAAGCC CCTGGAAAAG CACTACTCAG TTGTCCTGCC GACCGTGAGC 
CACAGTGGCT TCCTCTACAA GACTGCCTCT GCCGGCAAGC TGCTACAGGA CCGCCGGGCC 
CGGGAAGAGT TCAGCCGGCG CTGGTGTGTC CTTGGTGACG GGGTCCTGAG CTACTTTGAG 
AATGAGCGGG CAGTGACCCC CAATGGAGAG ATTCGGGCCA GCGAGATTGT GTGCCTGGCA 
GTGCCCCCTC CTGACACCCA TGGCTTTGAG CACACCTTTG AGGTGTACAC GGAGGGAGAA 
CGGCTGTACC TGTTTGGGCT GGAGAGTGCG GAGCAGGCTC ATGAGTGGGT CAAGTGTATT 
GCTAAGGCAT TCGTGCCTCC CCTAGCCGAG GATCTGCTGG CCCGGGATTT TGAGCGGCTG 
GGACGCCTAC CCTACAAAGC TGGCCTGAGC CTACAGCGGG CCCAGGAGGG CTGGTTCTCT 
CTCAGTGGCT CGGAGCTCCG TGCTGTCTTC CCGGAGGGGC CCTGCGAAGA GCCGCTGCAA 
CTACGGAAAC TGCAGGAGCT TTCCATCCAG GGGGACAGTG AGAACCAGGT GCTGGTGCTG 
GTGGAGCGAA GGAGGACACT GTACATACAG GGCGAGCGGC GGCTGGACTT CATGGGTTGG 
CTGGGGGCCA TCCAGAAAGC AGCCGCCAGC ATGGGGGACA CGCTGTCGGA GCAGCAGCTT 
GGGGACTCGG ATATCCCGGT GATCGTGTAC CGCTGTGTGG ACTACATCAC GCAGTGCGGC 
CTGACCTCCG AGGGCATCTA CCGCAAGTGT GGGCAGACAT CGAAGACACA GCGGCTGCTG 
GAGAGCCTGC GGCAGGATGC GCGCTCTGTG CACCTCAAGG AGGGCGAGCA GCACGTGGAT 
GATGTTTCCT CGGCGCTCAA GCGCTTCCTG CGCGACCTGC CTGATGGGCT CTTCACTCGC 
GCCCAGCGCC TAACCTGGCT GGAGGCCTCA GAGATTGAGG ACGAGGAGGA GAAGGTCTCC 
AGGTACCGAG AGCTGCTGGT GCGGCTGCCC CCTGTCAACC GGGCCACAGT GAAGGCCCTT 
ATCAGCCACC TGTACTGTGT TCAGTGCTTC TCAGACACGA ACCAGATGAA CGTGCACAAC 
CTGGCAATTG TGTTTGGGCC CACGCTCTTC CAGACAGATG GGCAGGACTA CAAGGCTGGC 
CGTGTGGTGG AAGACCTCAT TAACCACTAT GTGGTGGTGT TTAGTGTGGA TGAGGAAGAG 
CTCAGGAAGC AGCGGGAGGA GATCACTGCC ATTGTGAAGA TGCGCGTGGC TGGCACTGCC 
AGTGGGACCC AGCATGCCGG TGACTTCATC TGCACAGTGT ATCTGGAAGA GAAGAAGGCA 
GAGACTGAGC AGCATATCAA GGTCCCAGCA TCCATGACTG CTGAGGAGCT CACCCTGGAG 
ATCCTGGATC GCCGGAACGT GGGCATCAGG GAGAAGGACT ATTGGACCTG CTTTGAGGTC 
AACGAGAGGG AGGAGGCAGA GCGCCCCCTG CACTTTGCGG AGAAGGTGCT GCCCATCCTG 
CACGGGCTGG GCACGGACAG CCACCTGGTG GTGAAGAAGC ACCAGGCCAT GGAGGCCATG 
CTGCTGTACC TGGCCAGCCG TGTCGGTGAC ACCAAGCATG GCATGATGAA GTTCCGTGAG 
GACCGCAGCC TCCTGGGCCT GGGCCTGCCC TCAGGTGGCT TCCACGATCG CTACTTCATC 
CTCAACAGCA GCTGCTTGCG GCTCTACAAG GAGGTCCGGA GTCACCGGCC TGAGAAGGAG 
TGGCCTATTA AGAGTCTCAA AGTCTACCTG GGAGTGAAGA AGAAACTCAG GCCACCCACC 
TGCTGGGGCT TCACAGTGGT GCATGAGACA GAGAAACATG AGAAGCAGCA GTGGTACCTC 
TGCTGTGACA CACAGATGGA GCTCCGGGAG TGGTTCGCTA CCTTTCTGTT TGTGCAGCAT 
GACGGCCTGG TGTGGCCCTC AGAGCCCTCA CGCGTGTCCC GGGCAGTGCC TGAGGTCCGG 
CTGGGTAGTG TGTCACTGAT CCCCCTTCGA GGTAGTGAAA ATGAAATGCG CCGGAGTGTG 
GCTGCCTTCA CCGCGGACCC TCTGTCTCTT CTGCGCAACG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARAP1 ( NM_015242.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056057.2 Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 1, mRNA.

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CloneID	OHu15146
Clone ID Related Accession (Same CDS sequence)	NM_015242.4
Accession Version	NM_015242.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA186424.1, AB018325.2 and BC008315.1. On or before Sep 25, 2008 this sequence version replaced NM_139181.2, NM_015242.3. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 3. This difference causes translation initiation at a downstream AUG and an isoform (a) with a shorter N-terminus compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB018325.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCAAGA AGGAGGAGCC CCCACCGAGC CGAGTCCCAC GGGCCGTGCG CGTGGCCAGT 
CTGCTGAGCG AGGGAGAGGA ACTGTCTGGG GACGACCAAG GGGATGAGGA AGAGGATGAC 
CACGCCTATG AGGGCGTCCC CAATGGCGGA TGGCATACCA GCAGCCTGAG CTTGTCCTTG 
CCCAGCACAA TAGCTGCGCC ACACCCCATG GACGGGCCGC CTGGGGGCTC CACCCCCGTC 
ACACCAGTCA TCAAGGCTGG CTGGCTGGAC AAGAACCCAC CGCAGGGATC TTACATCTAT 
CAGAAACGAT GGGTGAGACT GGATACTGAT CACCTGCGAT ACTTTGACAG TAACAAGGAC 
GCTTACTCTA AGCGCTTTAT CTCTGTGGCC TGCATCTCCC ACGTGGCTGC CATCGGGGAC 
CAGAAGTTTG AAGTGATCAC AAACAACCGA ACCTTTGCCT TCCGGGCAGA GAGTGATGTG 
GAGCGGAAGG AGTGGATGCA GGCCCTGCAG CAGGCCATGG CTGAGCAGCG TGCCCGGGCC 
CGGCTCTCTA GCGCTTATCT GCTGGGAGTT CCAGGCTCAG AGCAGCCTGA CCGCGCTGGC 
AGCCTGGAGC TTCGTGGCTT CAAGAATAAG CTGTACGTGG CCGTGGTCGG GGACAAAGTG 
CAGCTCTACA AGAATCTAGA GGAGTACCAC CTGGGCATTG GCATCACCTT CATCGACATG 
AGCGTGGGCA ACGTGAAGGA AGTGGACCGG CGCAGCTTCG ACCTCACCAC GCCCTACCGC 
ATCTTCAGCT TCTCTGCTGA CTCAGAGCTA GAGAAGGAGC AGTGGTTGGA GGCCATGCAG 
GGAGCCATCG CTGAGGCCCT GTCTACCTCG GAGGTGGCCG AGCGCATCTG GGCTGCAGCC 
CCCAACAGGT TCTGTGCTGA CTGCGGGGCT CCTCAGCCTG ACTGGGCCTC CATCAACCTC 
TGTGTTGTTA TCTGCAAGCG CTGTGCAGGG GAGCACCGTG GCCTGGGCGC TGGCGTCTCC 
AAGGTGCGGA GCCTGAAGAT GGACAGGAAG GTGTGGACAG AAACACTTAT CGAGCTCTTC 
TTACAGCTGG GGAATGGCGC TGGGAACCGC TTCTGGGCAG CCAACGTGCC CCCCAGTGAG 
GCCCTGCAGC CCAGCAGCAG CCCCAGCACC CGGCGGTGCC ACCTGGAGGC CAAGTACCGT 
GAGGGCAAGT ACCGCCGCTA CCACCCGCTC TTTGGCAACC AGGAGGAGCT GGACAAGGCC 
CTGTGTGCTG CAGTCACCAC CACAGACCTG GCTGAGACCC AGGCGCTCCT GGGCTGTGGG 
GCTGGGATCA ACTGCTTCTC GGGGGACCCT GAGGCCCCCA CGCCCCTGGC TCTTGCAGAG 
CAGGCGGGGC AGACGCTGCA GATGGAATTC CTTCGGAACA ACCGGACCAC AGAGGTGCCT 
CGGCTGGACT CGATGAAGCC CCTGGAAAAG CACTACTCAG TTGTCCTGCC GACCGTGAGC 
CACAGTGGCT TCCTCTACAA GACTGCCTCT GCCGGCAAGC TGCTACAGGA CCGCCGGGCC 
CGGGAAGAGT TCAGCCGGCG CTGGTGTGTC CTTGGTGACG GGGTCCTGAG CTACTTTGAG 
AATGAGCGGG CAGTGACCCC CAATGGAGAG ATTCGGGCCA GCGAGATTGT GTGCCTGGCA 
GTGCCCCCTC CTGACACCCA TGGCTTTGAG CACACCTTTG AGGTGTACAC GGAGGGAGAA 
CGGCTGTACC TGTTTGGGCT GGAGAGTGCG GAGCAGGCTC ATGAGTGGGT CAAGTGTATT 
GCTAAGGCAT TCGTGCCTCC CCTAGCCGAG GATCTGCTGG CCCGGGATTT TGAGCGGCTG 
GGACGCCTAC CCTACAAAGC TGGCCTGAGC CTACAGCGGG CCCAGGAGGG CTGGTTCTCT 
CTCAGTGGCT CGGAGCTCCG TGCTGTCTTC CCGGAGGGGC CCTGCGAAGA GCCGCTGCAA 
CTACGGAAAC TGCAGGAGCT TTCCATCCAG GGGGACAGTG AGAACCAGGT GCTGGTGCTG 
GTGGAGCGAA GGAGGACACT GTACATACAG GGCGAGCGGC GGCTGGACTT CATGGGTTGG 
CTGGGGGCCA TCCAGAAAGC AGCCGCCAGC ATGGGGGACA CGCTGTCGGA GCAGCAGCTT 
GGGGACTCGG ATATCCCGGT GATCGTGTAC CGCTGTGTGG ACTACATCAC GCAGTGCGGC 
CTGACCTCCG AGGGCATCTA CCGCAAGTGT GGGCAGACAT CGAAGACACA GCGGCTGCTG 
GAGAGCCTGC GGCAGGATGC GCGCTCTGTG CACCTCAAGG AGGGCGAGCA GCACGTGGAT 
GATGTTTCCT CGGCGCTCAA GCGCTTCCTG CGCGACCTGC CTGATGGGCT CTTCACTCGC 
GCCCAGCGCC TAACCTGGCT GGAGGCCTCA GAGATTGAGG ACGAGGAGGA GAAGGTCTCC 
AGGTACCGAG AGCTGCTGGT GCGGCTGCCC CCTGTCAACC GGGCCACAGT GAAGGCCCTT 
ATCAGCCACC TGTACTGTGT TCAGTGCTTC TCAGACACGA ACCAGATGAA CGTGCACAAC 
CTGGCAATTG TGTTTGGGCC CACGCTCTTC CAGACAGATG GGCAGGACTA CAAGGCTGGC 
CGTGTGGTGG AAGACCTCAT TAACCACTAT GTGGTGGTGT TTAGTGTGGA TGAGGAAGAG 
CTCAGGAAGC AGCGGGAGGA GATCACTGCC ATTGTGAAGA TGCGCGTGGC TGGCACTGCC 
AGTGGGACCC AGCATGCCGG TGACTTCATC TGCACAGTGT ATCTGGAAGA GAAGAAGGCA 
GAGACTGAGC AGCATATCAA GGTCCCAGCA TCCATGACTG CTGAGGAGCT CACCCTGGAG 
ATCCTGGATC GCCGGAACGT GGGCATCAGG GAGAAGGACT ATTGGACCTG CTTTGAGGTC 
AACGAGAGGG AGGAGGCAGA GCGCCCCCTG CACTTTGCGG AGAAGGTGCT GCCCATCCTG 
CACGGGCTGG GCACGGACAG CCACCTGGTG GTGAAGAAGC ACCAGGCCAT GGAGGCCATG 
CTGCTGTACC TGGCCAGCCG TGTCGGTGAC ACCAAGCATG GCATGATGAA GTTCCGTGAG 
GACCGCAGCC TCCTGGGCCT GGGCCTGCCC TCAGGTGGCT TCCACGATCG CTACTTCATC 
CTCAACAGCA GCTGCTTGCG GCTCTACAAG GAGGTCCGGA GCCAGAGGCC GTGGAGCGGG 
GCCCCTGAGA CCAGTCACCG GCCTGAGAAG GAGTGGCCTA TTAAGAGTCT CAAAGTCTAC 
CTGGGAGTGA AGAAGAAACT CAGGCCACCC ACCTGCTGGG GCTTCACAGT GGTGCATGAG 
ACAGAGAAAC ATGAGAAGCA GCAGTGGTAC CTCTGCTGTG ACACACAGAT GGAGCTCCGG 
GAGTGGTTCG CTACCTTTCT GTTTGTGCAG CATGACGGCC TGGTGTGGCC CTCAGAGCCC 
TCACGCGTGT CCCGGGCAGT GCCTGAGGTC CGGCTGGGTA GTGTGTCACT GATCCCCCTT 
CGAGGTAGTG AAAATGAAAT GCGCCGGAGT GTGGCTGCCT TCACCGCGGA CCCTCTGTCT 
CTTCTGCGCA ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056057.2
CDS	736..4353
Translation	MTKKEEPPPSRVPRAVRVASLLSEGEELSGDDQGDEEEDDHAYEGVPNGGWHTSSLSLSLPSTIAAPHPMDGPPGGSTPVTPVIKAGWLDKNPPQGSYIYQKRWVRLDTDHLRYFDSNKDAYSKRFISVACISHVAAIGDQKFEVITNNRTFAFRAESDVERKEWMQALQQAMAEQRARARLSSAYLLGVPGSEQPDRAGSLELRGFKNKLYVAVVGDKVQLYKNLEEYHLGIGITFIDMSVGNVKEVDRRSFDLTTPYRIFSFSADSELEKEQWLEAMQGAIAEALSTSEVAERIWAAAPNRFCADCGAPQPDWASINLCVVICKRCAGEHRGLGAGVSKVRSLKMDRKVWTETLIELFLQLGNGAGNRFWAANVPPSEALQPSSSPSTRRCHLEAKYREGKYRRYHPLFGNQEELDKALCAAVTTTDLAETQALLGCGAGINCFSGDPEAPTPLALAEQAGQTLQMEFLRNNRTTEVPRLDSMKPLEKHYSVVLPTVSHSGFLYKTASAGKLLQDRRAREEFSRRWCVLGDGVLSYFENERAVTPNGEIRASEIVCLAVPPPDTHGFEHTFEVYTEGERLYLFGLESAEQAHEWVKCIAKAFVPPLAEDLLARDFERLGRLPYKAGLSLQRAQEGWFSLSGSELRAVFPEGPCEEPLQLRKLQELSIQGDSENQVLVLVERRRTLYIQGERRLDFMGWLGAIQKAAASMGDTLSEQQLGDSDIPVIVYRCVDYITQCGLTSEGIYRKCGQTSKTQRLLESLRQDARSVHLKEGEQHVDDVSSALKRFLRDLPDGLFTRAQRLTWLEASEIEDEEEKVSRYRELLVRLPPVNRATVKALISHLYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFSVDEEELRKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIREKDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSQRPWSGAPETSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRVSRAVPEVRLGSVSLIPLRGSENEMRRSVAAFTADPLSLLRNV

Target ORF information:

RefSeq Version	NM_015242.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015242.4

ORF Insert Sequence:

ATGACCAAGA AGGAGGAGCC CCCACCGAGC CGAGTCCCAC GGGCCGTGCG CGTGGCCAGT 
CTGCTGAGCG AGGGAGAGGA ACTGTCTGGG GACGACCAAG GGGATGAGGA AGAGGATGAC 
CACGCCTATG AGGGCGTCCC CAATGGCGGA TGGCATACCA GCAGCCTGAG CTTGTCCTTG 
CCCAGCACAA TAGCTGCGCC ACACCCCATG GACGGGCCGC CTGGGGGCTC CACCCCCGTC 
ACACCAGTCA TCAAGGCTGG CTGGCTGGAC AAGAACCCAC CGCAGGGATC TTACATCTAT 
CAGAAACGAT GGGTGAGACT GGATACTGAT CACCTGCGAT ACTTTGACAG TAACAAGGAC 
GCTTACTCTA AGCGCTTTAT CTCTGTGGCC TGCATCTCCC ACGTGGCTGC CATCGGGGAC 
CAGAAGTTTG AAGTGATCAC AAACAACCGA ACCTTTGCCT TCCGGGCAGA GAGTGATGTG 
GAGCGGAAGG AGTGGATGCA GGCCCTGCAG CAGGCCATGG CTGAGCAGCG TGCCCGGGCC 
CGGCTCTCTA GCGCTTATCT GCTGGGAGTT CCAGGCTCAG AGCAGCCTGA CCGCGCTGGC 
AGCCTGGAGC TTCGTGGCTT CAAGAATAAG CTGTACGTGG CCGTGGTCGG GGACAAAGTG 
CAGCTCTACA AGAATCTAGA GGAGTACCAC CTGGGCATTG GCATCACCTT CATCGACATG 
AGCGTGGGCA ACGTGAAGGA AGTGGACCGG CGCAGCTTCG ACCTCACCAC GCCCTACCGC 
ATCTTCAGCT TCTCTGCTGA CTCAGAGCTA GAGAAGGAGC AGTGGTTGGA GGCCATGCAG 
GGAGCCATCG CTGAGGCCCT GTCTACCTCG GAGGTGGCCG AGCGCATCTG GGCTGCAGCC 
CCCAACAGGT TCTGTGCTGA CTGCGGGGCT CCTCAGCCTG ACTGGGCCTC CATCAACCTC 
TGTGTTGTTA TCTGCAAGCG CTGTGCAGGG GAGCACCGTG GCCTGGGCGC TGGCGTCTCC 
AAGGTGCGGA GCCTGAAGAT GGACAGGAAG GTGTGGACAG AAACACTTAT CGAGCTCTTC 
TTACAGCTGG GGAATGGCGC TGGGAACCGC TTCTGGGCAG CCAACGTGCC CCCCAGTGAG 
GCCCTGCAGC CCAGCAGCAG CCCCAGCACC CGGCGGTGCC ACCTGGAGGC CAAGTACCGT 
GAGGGCAAGT ACCGCCGCTA CCACCCGCTC TTTGGCAACC AGGAGGAGCT GGACAAGGCC 
CTGTGTGCTG CAGTCACCAC CACAGACCTG GCTGAGACCC AGGCGCTCCT GGGCTGTGGG 
GCTGGGATCA ACTGCTTCTC GGGGGACCCT GAGGCCCCCA CGCCCCTGGC TCTTGCAGAG 
CAGGCGGGGC AGACGCTGCA GATGGAATTC CTTCGGAACA ACCGGACCAC AGAGGTGCCT 
CGGCTGGACT CGATGAAGCC CCTGGAAAAG CACTACTCAG TTGTCCTGCC GACCGTGAGC 
CACAGTGGCT TCCTCTACAA GACTGCCTCT GCCGGCAAGC TGCTACAGGA CCGCCGGGCC 
CGGGAAGAGT TCAGCCGGCG CTGGTGTGTC CTTGGTGACG GGGTCCTGAG CTACTTTGAG 
AATGAGCGGG CAGTGACCCC CAATGGAGAG ATTCGGGCCA GCGAGATTGT GTGCCTGGCA 
GTGCCCCCTC CTGACACCCA TGGCTTTGAG CACACCTTTG AGGTGTACAC GGAGGGAGAA 
CGGCTGTACC TGTTTGGGCT GGAGAGTGCG GAGCAGGCTC ATGAGTGGGT CAAGTGTATT 
GCTAAGGCAT TCGTGCCTCC CCTAGCCGAG GATCTGCTGG CCCGGGATTT TGAGCGGCTG 
GGACGCCTAC CCTACAAAGC TGGCCTGAGC CTACAGCGGG CCCAGGAGGG CTGGTTCTCT 
CTCAGTGGCT CGGAGCTCCG TGCTGTCTTC CCGGAGGGGC CCTGCGAAGA GCCGCTGCAA 
CTACGGAAAC TGCAGGAGCT TTCCATCCAG GGGGACAGTG AGAACCAGGT GCTGGTGCTG 
GTGGAGCGAA GGAGGACACT GTACATACAG GGCGAGCGGC GGCTGGACTT CATGGGTTGG 
CTGGGGGCCA TCCAGAAAGC AGCCGCCAGC ATGGGGGACA CGCTGTCGGA GCAGCAGCTT 
GGGGACTCGG ATATCCCGGT GATCGTGTAC CGCTGTGTGG ACTACATCAC GCAGTGCGGC 
CTGACCTCCG AGGGCATCTA CCGCAAGTGT GGGCAGACAT CGAAGACACA GCGGCTGCTG 
GAGAGCCTGC GGCAGGATGC GCGCTCTGTG CACCTCAAGG AGGGCGAGCA GCACGTGGAT 
GATGTTTCCT CGGCGCTCAA GCGCTTCCTG CGCGACCTGC CTGATGGGCT CTTCACTCGC 
GCCCAGCGCC TAACCTGGCT GGAGGCCTCA GAGATTGAGG ACGAGGAGGA GAAGGTCTCC 
AGGTACCGAG AGCTGCTGGT GCGGCTGCCC CCTGTCAACC GGGCCACAGT GAAGGCCCTT 
ATCAGCCACC TGTACTGTGT TCAGTGCTTC TCAGACACGA ACCAGATGAA CGTGCACAAC 
CTGGCAATTG TGTTTGGGCC CACGCTCTTC CAGACAGATG GGCAGGACTA CAAGGCTGGC 
CGTGTGGTGG AAGACCTCAT TAACCACTAT GTGGTGGTGT TTAGTGTGGA TGAGGAAGAG 
CTCAGGAAGC AGCGGGAGGA GATCACTGCC ATTGTGAAGA TGCGCGTGGC TGGCACTGCC 
AGTGGGACCC AGCATGCCGG TGACTTCATC TGCACAGTGT ATCTGGAAGA GAAGAAGGCA 
GAGACTGAGC AGCATATCAA GGTCCCAGCA TCCATGACTG CTGAGGAGCT CACCCTGGAG 
ATCCTGGATC GCCGGAACGT GGGCATCAGG GAGAAGGACT ATTGGACCTG CTTTGAGGTC 
AACGAGAGGG AGGAGGCAGA GCGCCCCCTG CACTTTGCGG AGAAGGTGCT GCCCATCCTG 
CACGGGCTGG GCACGGACAG CCACCTGGTG GTGAAGAAGC ACCAGGCCAT GGAGGCCATG 
CTGCTGTACC TGGCCAGCCG TGTCGGTGAC ACCAAGCATG GCATGATGAA GTTCCGTGAG 
GACCGCAGCC TCCTGGGCCT GGGCCTGCCC TCAGGTGGCT TCCACGATCG CTACTTCATC 
CTCAACAGCA GCTGCTTGCG GCTCTACAAG GAGGTCCGGA GCCAGAGGCC GTGGAGCGGG 
GCCCCTGAGA CCAGTCACCG GCCTGAGAAG GAGTGGCCTA TTAAGAGTCT CAAAGTCTAC 
CTGGGAGTGA AGAAGAAACT CAGGCCACCC ACCTGCTGGG GCTTCACAGT GGTGCATGAG 
ACAGAGAAAC ATGAGAAGCA GCAGTGGTAC CTCTGCTGTG ACACACAGAT GGAGCTCCGG 
GAGTGGTTCG CTACCTTTCT GTTTGTGCAG CATGACGGCC TGGTGTGGCC CTCAGAGCCC 
TCACGCGTGT CCCGGGCAGT GCCTGAGGTC CGGCTGGGTA GTGTGTCACT GATCCCCCTT 
CGAGGTAGTG AAAATGAAAT GCGCCGGAGT GTGGCTGCCT TCACCGCGGA CCCTCTGTCT 
CTTCTGCGCA ACGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARAP1 ( NM_001135190.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128662.1 Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 4, mRNA.

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CloneID	OHu15209
Clone ID Related Accession (Same CDS sequence)	NM_001135190.1
Accession Version	NM_001135190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA186424.1, BC140792.1, AP003065.2 and BC008315.1. Transcript Variant: This variant (4) differs in the 5' UTR and lacks two alternate in-frame coding exons, compared to variant 3. the resulting protein (isoform d) has a shorter N-terminus and lacks the SAM domain and portions of the ArfGAP and fifth PH domains, compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC140792.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCAAGA AGGAGGAGCC CCCACCGAGC CGAGTCCCAC GGGCCGTGCG CGTGGCCAGT 
CTGCTGAGCG AGGGAGAGGA ACTGTCTGGG GACGACCAAG GGGATGAGGA AGAGGATGAC 
CACGCCTATG AGGGCGTCCC CAATGGCGGA TGGCATACCA GCAGCCTGAG CTTGTCCTTG 
CCCAGCACAA TAGCTGCGCC ACACCCCATG GACGGGCCGC CTGGGGGCTC CACCCCCGTC 
ACACCAGTCA TCAAGGCTGG CTGGCTGGAC AAGAACCCAC CGCAGGGATC TTACATCTAT 
CAGAAACGAT GGGTGAGACT GGATACTGAT CACCTGCGAT ACTTTGACAG TAACAAGGAC 
GCTTACTCTA AGCGCTTTAT CTCTGTGGCC TGCATCTCCC ACGTGGCTGC CATCGGGGAC 
CAGAAGTTTG AAGTGATCAC AAACAACCGA ACCTTTGCCT TCCGGGCAGA GAGTGATGTG 
GAGCGGAAGG AGTGGATGCA GGCCCTGCAG CAGGCCATGG CTGAGCAGCG TGCCCGGGCC 
CGGCTCTCTA GCGCTTATCT GCTGGGAGTT CCAGGCTCAG AGCAGCCTGA CCGCGCTGGC 
AGCCTGGAGC TTCGTGGCTT CAAGAATAAG CTGTACGTGG CCGTGGTCGG GGACAAAGTG 
CAGCTCTACA AGAATCTAGA GGAGTACCAC CTGGGCATTG GCATCACCTT CATCGACATG 
AGCGTGGGCA ACGTGAAGGA AGTGGACCGG CGCAGCTTCG ACCTCACCAC GCCCTACCGC 
ATCTTCAGCT TCTCTGCTGA CTCAGAGCTA GAGAAGGAGC AGTGGTTGGA GGCCATGCAG 
GGAGCCATCG CTGAGGCCCT GTCTACCTCG GAGGTGGCCG AGCGCATCTG GGCTGCAGCC 
CCCAACAGGT TCTGTGCTGA CTGCGGGGCT CCTCAGCCTG ACTGGGCCTC CATCAACCTC 
TGTGTTGTTA TCTGCAAGCG CTGTGCAGGG GAGCACCGTG GCCTGGGCGC TGGCGTCTCC 
AAGGTGCGGA GCCTGAAGAT GGACAGGAAG GTGTGGACAG AAACACTTAT CGAGGCCCTG 
TGTGCTGCAG TCACCACCAC AGACCTGGCT GAGACCCAGG CGCTCCTGGG CTGTGGGGCT 
GGGATCAACT GCTTCTCGGG GGACCCTGAG GCCCCCACGC CCCTGGCTCT TGCAGAGCAG 
GCGGGGCAGA CGCTGCAGAT GGAATTCCTT CGGAACAACC GGACCACAGA GGTGCCTCGG 
CTGGACTCGA TGAAGCCCCT GGAAAAGCAC TACTCAGTTG TCCTGCCGAC CGTGAGCCAC 
AGTGGCTTCC TCTACAAGAC TGCCTCTGCC GGCAAGCTGC TACAGGACCG CCGGGCCCGG 
GAAGAGTTCA GCCGGCGCTG GTGTGTCCTT GGTGACGGGG TCCTGAGCTA CTTTGAGAAT 
GAGCGGGCAG TGACCCCCAA TGGAGAGATT CGGGCCAGCG AGATTGTGTG CCTGGCAGTG 
CCCCCTCCTG ACACCCATGG CTTTGAGCAC ACCTTTGAGG TGTACACGGA GGGAGAACGG 
CTGTACCTGT TTGGGCTGGA GAGTGCGGAG CAGGCTCATG AGTGGGTCAA GTGTATTGCT 
AAGGCATTCG TGCCTCCCCT AGCCGAGGAT CTGCTGGCCC GGGATTTTGA GCGGCTGGGA 
CGCCTACCCT ACAAAGCTGG CCTGAGCCTA CAGCGGGCCC AGGAGGGCTG GTTCTCTCTC 
AGTGGCTCGG AGCTCCGTGC TGTCTTCCCG GAGGGGCCCT GCGAAGAGCC GCTGCAACTA 
CGGAAACTGC AGGAGCTTTC CATCCAGGGG GACAGTGAGA ACCAGGTGCT GGTGCTGGTG 
GAGCGAAGGA GGACACTGTA CATACAGGGC GAGCGGCGGC TGGACTTCAT GGGTTGGCTG 
GGGGCCATCC AGAAAGCAGC CGCCAGCATG GGGGACACGC TGTCGGAGCA GCAGCTTGGG 
GACTCGGATA TCCCGGTGAT CGTGTACCGC TGTGTGGACT ACATCACGCA GTGCGGCCTG 
ACCTCCGAGG GCATCTACCG CAAGTGTGGG CAGACATCGA AGACACAGCG GCTGCTGGAG 
AGCCTGCGGC AGGATGCGCG CTCTGTGCAC CTCAAGGAGG GCGAGCAGCA CGTGGATGAT 
GTTTCCTCGG CGCTCAAGCG CTTCCTGCGC GACCTGCCTG ATGGGCTCTT CACTCGCGCC 
CAGCGCCTAA CCTGGCTGGA GGCCTCAGAG ATTGAGGACG AGGAGGAGAA GGTCTCCAGG 
TACCGAGAGC TGCTGGTGCG GCTGCCCCCT GTCAACCGGG CCACAGTGAA GGCCCTTATC 
AGCCACCTGT ACTGTGTTCA GTGCTTCTCA GACACGAACC AGATGAACGT GCACAACCTG 
GCAATTGTGT TTGGGCCCAC GCTCTTCCAG ACAGATGGGC AGGACTACAA GGCTGGCCGT 
GTGGTGGAAG ACCTCATTAA CCACTATGTG GTGGTGTTTA GTGTGGATGA GGAAGAGCTC 
AGGAAGCAGC GGGAGGAGAT CACTGCCATT GTGAAGATGC GCGTGGCTGG CACTGCCAGT 
GGGACCCAGC ATGCCGGTGA CTTCATCTGC ACAGTGTATC TGGAAGAGAA GAAGGCAGAG 
ACTGAGCAGC ATATCAAGGT CCCAGCATCC ATGACTGCTG AGGAGCTCAC CCTGGAGATC 
CTGGATCGCC GGAACGTGGG CATCAGGGAG AAGGACTATT GGACCTGCTT TGAGGTCAAC 
GAGAGGGAGG AGGCAGAGCG CCCCCTGCAC TTTGCGGAGA AGGTGCTGCC CATCCTGCAC 
GGGCTGGGCA CGGACAGCCA CCTGGTGGTG AAGAAGCACC AGGCCATGGA GGCCATGCTG 
CTGTACCTGG CCAGCCGTGT CGGTGACACC AAGCATGGCA TGATGAAGTT CCGTGAGGAC 
CGCAGCCTCC TGGGCCTGGG CCTGCCCTCA GGTGGCTTCC ACGATCGCTA CTTCATCCTC 
AACAGCAGCT GCTTGCGGCT CTACAAGGAG GTCCGGAGTC ACCGGCCTGA GAAGGAGTGG 
CCTATTAAGA GTCTCAAAGT CTACCTGGGA GTGAAGAAGA AACTCAGGCC ACCCACCTGC 
TGGGGCTTCA CAGTGGTGCA TGAGACAGAG AAACATGAGA AGCAGCAGTG GTACCTCTGC 
TGTGACACAC AGATGGAGCT CCGGGAGTGG TTCGCTACCT TTCTGTTTGT GCAGCATGAC 
GGCCTGGTGT GGCCCTCAGA GCCCTCACGC GTGTCCCGGG CAGTGCCTGA GGTCCGGCTG 
GGTAGTGTGT CACTGATCCC CCTTCGAGGT AGTGAAAATG AAATGCGCCG GAGTGTGGCT 
GCCTTCACCG CGGACCCTCT GTCTCTTCTG CGCAACGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128662.1
CDS	736..4137
Translation	MTKKEEPPPSRVPRAVRVASLLSEGEELSGDDQGDEEEDDHAYEGVPNGGWHTSSLSLSLPSTIAAPHPMDGPPGGSTPVTPVIKAGWLDKNPPQGSYIYQKRWVRLDTDHLRYFDSNKDAYSKRFISVACISHVAAIGDQKFEVITNNRTFAFRAESDVERKEWMQALQQAMAEQRARARLSSAYLLGVPGSEQPDRAGSLELRGFKNKLYVAVVGDKVQLYKNLEEYHLGIGITFIDMSVGNVKEVDRRSFDLTTPYRIFSFSADSELEKEQWLEAMQGAIAEALSTSEVAERIWAAAPNRFCADCGAPQPDWASINLCVVICKRCAGEHRGLGAGVSKVRSLKMDRKVWTETLIEALCAAVTTTDLAETQALLGCGAGINCFSGDPEAPTPLALAEQAGQTLQMEFLRNNRTTEVPRLDSMKPLEKHYSVVLPTVSHSGFLYKTASAGKLLQDRRAREEFSRRWCVLGDGVLSYFENERAVTPNGEIRASEIVCLAVPPPDTHGFEHTFEVYTEGERLYLFGLESAEQAHEWVKCIAKAFVPPLAEDLLARDFERLGRLPYKAGLSLQRAQEGWFSLSGSELRAVFPEGPCEEPLQLRKLQELSIQGDSENQVLVLVERRRTLYIQGERRLDFMGWLGAIQKAAASMGDTLSEQQLGDSDIPVIVYRCVDYITQCGLTSEGIYRKCGQTSKTQRLLESLRQDARSVHLKEGEQHVDDVSSALKRFLRDLPDGLFTRAQRLTWLEASEIEDEEEKVSRYRELLVRLPPVNRATVKALISHLYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFSVDEEELRKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIREKDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRVSRAVPEVRLGSVSLIPLRGSENEMRRSVAAFTADPLSLLRNV

Target ORF information:

RefSeq Version	NM_001135190.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135190.1

ORF Insert Sequence:

ATGACCAAGA AGGAGGAGCC CCCACCGAGC CGAGTCCCAC GGGCCGTGCG CGTGGCCAGT 
CTGCTGAGCG AGGGAGAGGA ACTGTCTGGG GACGACCAAG GGGATGAGGA AGAGGATGAC 
CACGCCTATG AGGGCGTCCC CAATGGCGGA TGGCATACCA GCAGCCTGAG CTTGTCCTTG 
CCCAGCACAA TAGCTGCGCC ACACCCCATG GACGGGCCGC CTGGGGGCTC CACCCCCGTC 
ACACCAGTCA TCAAGGCTGG CTGGCTGGAC AAGAACCCAC CGCAGGGATC TTACATCTAT 
CAGAAACGAT GGGTGAGACT GGATACTGAT CACCTGCGAT ACTTTGACAG TAACAAGGAC 
GCTTACTCTA AGCGCTTTAT CTCTGTGGCC TGCATCTCCC ACGTGGCTGC CATCGGGGAC 
CAGAAGTTTG AAGTGATCAC AAACAACCGA ACCTTTGCCT TCCGGGCAGA GAGTGATGTG 
GAGCGGAAGG AGTGGATGCA GGCCCTGCAG CAGGCCATGG CTGAGCAGCG TGCCCGGGCC 
CGGCTCTCTA GCGCTTATCT GCTGGGAGTT CCAGGCTCAG AGCAGCCTGA CCGCGCTGGC 
AGCCTGGAGC TTCGTGGCTT CAAGAATAAG CTGTACGTGG CCGTGGTCGG GGACAAAGTG 
CAGCTCTACA AGAATCTAGA GGAGTACCAC CTGGGCATTG GCATCACCTT CATCGACATG 
AGCGTGGGCA ACGTGAAGGA AGTGGACCGG CGCAGCTTCG ACCTCACCAC GCCCTACCGC 
ATCTTCAGCT TCTCTGCTGA CTCAGAGCTA GAGAAGGAGC AGTGGTTGGA GGCCATGCAG 
GGAGCCATCG CTGAGGCCCT GTCTACCTCG GAGGTGGCCG AGCGCATCTG GGCTGCAGCC 
CCCAACAGGT TCTGTGCTGA CTGCGGGGCT CCTCAGCCTG ACTGGGCCTC CATCAACCTC 
TGTGTTGTTA TCTGCAAGCG CTGTGCAGGG GAGCACCGTG GCCTGGGCGC TGGCGTCTCC 
AAGGTGCGGA GCCTGAAGAT GGACAGGAAG GTGTGGACAG AAACACTTAT CGAGGCCCTG 
TGTGCTGCAG TCACCACCAC AGACCTGGCT GAGACCCAGG CGCTCCTGGG CTGTGGGGCT 
GGGATCAACT GCTTCTCGGG GGACCCTGAG GCCCCCACGC CCCTGGCTCT TGCAGAGCAG 
GCGGGGCAGA CGCTGCAGAT GGAATTCCTT CGGAACAACC GGACCACAGA GGTGCCTCGG 
CTGGACTCGA TGAAGCCCCT GGAAAAGCAC TACTCAGTTG TCCTGCCGAC CGTGAGCCAC 
AGTGGCTTCC TCTACAAGAC TGCCTCTGCC GGCAAGCTGC TACAGGACCG CCGGGCCCGG 
GAAGAGTTCA GCCGGCGCTG GTGTGTCCTT GGTGACGGGG TCCTGAGCTA CTTTGAGAAT 
GAGCGGGCAG TGACCCCCAA TGGAGAGATT CGGGCCAGCG AGATTGTGTG CCTGGCAGTG 
CCCCCTCCTG ACACCCATGG CTTTGAGCAC ACCTTTGAGG TGTACACGGA GGGAGAACGG 
CTGTACCTGT TTGGGCTGGA GAGTGCGGAG CAGGCTCATG AGTGGGTCAA GTGTATTGCT 
AAGGCATTCG TGCCTCCCCT AGCCGAGGAT CTGCTGGCCC GGGATTTTGA GCGGCTGGGA 
CGCCTACCCT ACAAAGCTGG CCTGAGCCTA CAGCGGGCCC AGGAGGGCTG GTTCTCTCTC 
AGTGGCTCGG AGCTCCGTGC TGTCTTCCCG GAGGGGCCCT GCGAAGAGCC GCTGCAACTA 
CGGAAACTGC AGGAGCTTTC CATCCAGGGG GACAGTGAGA ACCAGGTGCT GGTGCTGGTG 
GAGCGAAGGA GGACACTGTA CATACAGGGC GAGCGGCGGC TGGACTTCAT GGGTTGGCTG 
GGGGCCATCC AGAAAGCAGC CGCCAGCATG GGGGACACGC TGTCGGAGCA GCAGCTTGGG 
GACTCGGATA TCCCGGTGAT CGTGTACCGC TGTGTGGACT ACATCACGCA GTGCGGCCTG 
ACCTCCGAGG GCATCTACCG CAAGTGTGGG CAGACATCGA AGACACAGCG GCTGCTGGAG 
AGCCTGCGGC AGGATGCGCG CTCTGTGCAC CTCAAGGAGG GCGAGCAGCA CGTGGATGAT 
GTTTCCTCGG CGCTCAAGCG CTTCCTGCGC GACCTGCCTG ATGGGCTCTT CACTCGCGCC 
CAGCGCCTAA CCTGGCTGGA GGCCTCAGAG ATTGAGGACG AGGAGGAGAA GGTCTCCAGG 
TACCGAGAGC TGCTGGTGCG GCTGCCCCCT GTCAACCGGG CCACAGTGAA GGCCCTTATC 
AGCCACCTGT ACTGTGTTCA GTGCTTCTCA GACACGAACC AGATGAACGT GCACAACCTG 
GCAATTGTGT TTGGGCCCAC GCTCTTCCAG ACAGATGGGC AGGACTACAA GGCTGGCCGT 
GTGGTGGAAG ACCTCATTAA CCACTATGTG GTGGTGTTTA GTGTGGATGA GGAAGAGCTC 
AGGAAGCAGC GGGAGGAGAT CACTGCCATT GTGAAGATGC GCGTGGCTGG CACTGCCAGT 
GGGACCCAGC ATGCCGGTGA CTTCATCTGC ACAGTGTATC TGGAAGAGAA GAAGGCAGAG 
ACTGAGCAGC ATATCAAGGT CCCAGCATCC ATGACTGCTG AGGAGCTCAC CCTGGAGATC 
CTGGATCGCC GGAACGTGGG CATCAGGGAG AAGGACTATT GGACCTGCTT TGAGGTCAAC 
GAGAGGGAGG AGGCAGAGCG CCCCCTGCAC TTTGCGGAGA AGGTGCTGCC CATCCTGCAC 
GGGCTGGGCA CGGACAGCCA CCTGGTGGTG AAGAAGCACC AGGCCATGGA GGCCATGCTG 
CTGTACCTGG CCAGCCGTGT CGGTGACACC AAGCATGGCA TGATGAAGTT CCGTGAGGAC 
CGCAGCCTCC TGGGCCTGGG CCTGCCCTCA GGTGGCTTCC ACGATCGCTA CTTCATCCTC 
AACAGCAGCT GCTTGCGGCT CTACAAGGAG GTCCGGAGTC ACCGGCCTGA GAAGGAGTGG 
CCTATTAAGA GTCTCAAAGT CTACCTGGGA GTGAAGAAGA AACTCAGGCC ACCCACCTGC 
TGGGGCTTCA CAGTGGTGCA TGAGACAGAG AAACATGAGA AGCAGCAGTG GTACCTCTGC 
TGTGACACAC AGATGGAGCT CCGGGAGTGG TTCGCTACCT TTCTGTTTGT GCAGCATGAC 
GGCCTGGTGT GGCCCTCAGA GCCCTCACGC GTGTCCCGGG CAGTGCCTGA GGTCCGGCTG 
GGTAGTGTGT CACTGATCCC CCTTCGAGGT AGTGAAAATG AAATGCGCCG GAGTGTGGCT 
GCCTTCACCG CGGACCCTCT GTCTCTTCTG CGCAACGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARAP1 ( NM_001040118.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035207.1 Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 3, mRNA.

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CloneID	OHu14897
Clone ID Related Accession (Same CDS sequence)	NM_001040118.2
Accession Version	NM_001040118.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY553630.1 and BC008315.1. On Sep 25, 2008 this sequence version replaced NM_001040118.1. Transcript Variant: This variant (3) is the longest transcript and it encodes the longest protein (isoform c). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY553630.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAGG CTGGGGATGC TGCGCTATCG GTGGCCGAGT GGCTGCGGGC ATTGCACCTG 
GAGCAGTACA CGGGGCTCTT TGAGCAGCAT GGCCTGGTGT GGGCCACTGA GTGCCAAGGC 
CTCAGCGACA CCCGCCTGAT GGACATGGGC ATGCTACTCC CTGGTCACCG CCGCCGCATC 
CTGGCTGGCC TGCTCCGTGC CCATACCTCA CCGGCCCCTG CACCCCGCCC CACCCCACGG 
CCTGTGCCCA TGAAGCGCCA CATCTTCCGC TCACCACCTG TGCCTGCCAC TCCACCCGAG 
CCGCTGCCCA CCACTACAGA GGATGAGGGG CTCCCCGCTG CCCCACCCAT CCCGCCCCGG 
AGGAGCTGCC TTCCGCCCAC CTGCTTCACC ACCCCATCCA CAGCTGCCCC AGACCCTGTG 
CTGCCCCCGC TGCCTGCTAA GCGGCATTTG GCAGAGCTGA GCGTTCCACC CGTGCCGCCC 
CGCACCGGAC CCCCCCGCCT GCTGGTGAGC CTGCCCACTA AGGAGGAGGA GTCATTGCTG 
CCATCATTAT CATCCCCTCC CCAGCCACAG TCTGAGGAGC CCCTGTCCAC CCTCCCCCAG 
GGGCCTCCCC AGCCTCCCTC TCCACCTCCC TGCCCCCCGG AGATACCTCC AAAGCCGGTA 
CGCCTGTTCC CAGAGTTCGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGGGG 
GCCCCAGCCA GAGTGATGAC CAAGAAGGAG GAGCCCCCAC CGAGCCGAGT CCCACGGGCC 
GTGCGCGTGG CCAGTCTGCT GAGCGAGGGA GAGGAACTGT CTGGGGACGA CCAAGGGGAT 
GAGGAAGAGG ATGACCACGC CTATGAGGGC GTCCCCAATG GCGGATGGCA TACCAGCAGC 
CTGAGCTTGT CCTTGCCCAG CACAATAGCT GCGCCACACC CCATGGACGG GCCGCCTGGG 
GGCTCCACCC CCGTCACACC AGTCATCAAG GCTGGCTGGC TGGACAAGAA CCCACCGCAG 
GGATCTTACA TCTATCAGAA ACGATGGGTG AGACTGGATA CTGATCACCT GCGATACTTT 
GACAGTAACA AGGACGCTTA CTCTAAGCGC TTTATCTCTG TGGCCTGCAT CTCCCACGTG 
GCTGCCATCG GGGACCAGAA GTTTGAAGTG ATCACAAACA ACCGAACCTT TGCCTTCCGG 
GCAGAGAGTG ATGTGGAGCG GAAGGAGTGG ATGCAGGCCC TGCAGCAGGC CATGGCTGAG 
CAGCGTGCCC GGGCCCGGCT CTCTAGCGCT TATCTGCTGG GAGTTCCAGG CTCAGAGCAG 
CCTGACCGCG CTGGCAGCCT GGAGCTTCGT GGCTTCAAGA ATAAGCTGTA CGTGGCCGTG 
GTCGGGGACA AAGTGCAGCT CTACAAGAAT CTAGAGGAGT ACCACCTGGG CATTGGCATC 
ACCTTCATCG ACATGAGCGT GGGCAACGTG AAGGAAGTGG ACCGGCGCAG CTTCGACCTC 
ACCACGCCCT ACCGCATCTT CAGCTTCTCT GCTGACTCAG AGCTAGAGAA GGAGCAGTGG 
TTGGAGGCCA TGCAGGGAGC CATCGCTGAG GCCCTGTCTA CCTCGGAGGT GGCCGAGCGC 
ATCTGGGCTG CAGCCCCCAA CAGGTTCTGT GCTGACTGCG GGGCTCCTCA GCCTGACTGG 
GCCTCCATCA ACCTCTGTGT TGTTATCTGC AAGCGCTGTG CAGGGGAGCA CCGTGGCCTG 
GGCGCTGGCG TCTCCAAGGT GCGGAGCCTG AAGATGGACA GGAAGGTGTG GACAGAAACA 
CTTATCGAGC TCTTCTTACA GCTGGGGAAT GGCGCTGGGA ACCGCTTCTG GGCAGCCAAC 
GTGCCCCCCA GTGAGGCCCT GCAGCCCAGC AGCAGCCCCA GCACCCGGCG GTGCCACCTG 
GAGGCCAAGT ACCGTGAGGG CAAGTACCGC CGCTACCACC CGCTCTTTGG CAACCAGGAG 
GAGCTGGACA AGGCCCTGTG TGCTGCAGTC ACCACCACAG ACCTGGCTGA GACCCAGGCG 
CTCCTGGGCT GTGGGGCTGG GATCAACTGC TTCTCGGGGG ACCCTGAGGC CCCCACGCCC 
CTGGCTCTTG CAGAGCAGGC GGGGCAGACG CTGCAGATGG AATTCCTTCG GAACAACCGG 
ACCACAGAGG TGCCTCGGCT GGACTCGATG AAGCCCCTGG AAAAGCACTA CTCAGTTGTC 
CTGCCGACCG TGAGCCACAG TGGCTTCCTC TACAAGACTG CCTCTGCCGG CAAGCTGCTA 
CAGGACCGCC GGGCCCGGGA AGAGTTCAGC CGGCGCTGGT GTGTCCTTGG TGACGGGGTC 
CTGAGCTACT TTGAGAATGA GCGGGCAGTG ACCCCCAATG GAGAGATTCG GGCCAGCGAG 
ATTGTGTGCC TGGCAGTGCC CCCTCCTGAC ACCCATGGCT TTGAGCACAC CTTTGAGGTG 
TACACGGAGG GAGAACGGCT GTACCTGTTT GGGCTGGAGA GTGCGGAGCA GGCTCATGAG 
TGGGTCAAGT GTATTGCTAA GGCATTCGTG CCTCCCCTAG CCGAGGATCT GCTGGCCCGG 
GATTTTGAGC GGCTGGGACG CCTACCCTAC AAAGCTGGCC TGAGCCTACA GCGGGCCCAG 
GAGGGCTGGT TCTCTCTCAG TGGCTCGGAG CTCCGTGCTG TCTTCCCGGA GGGGCCCTGC 
GAAGAGCCGC TGCAACTACG GAAACTGCAG GAGCTTTCCA TCCAGGGGGA CAGTGAGAAC 
CAGGTGCTGG TGCTGGTGGA GCGAAGGAGG ACACTGTACA TACAGGGCGA GCGGCGGCTG 
GACTTCATGG GTTGGCTGGG GGCCATCCAG AAAGCAGCCG CCAGCATGGG GGACACGCTG 
TCGGAGCAGC AGCTTGGGGA CTCGGATATC CCGGTGATCG TGTACCGCTG TGTGGACTAC 
ATCACGCAGT GCGGCCTGAC CTCCGAGGGC ATCTACCGCA AGTGTGGGCA GACATCGAAG 
ACACAGCGGC TGCTGGAGAG CCTGCGGCAG GATGCGCGCT CTGTGCACCT CAAGGAGGGC 
GAGCAGCACG TGGATGATGT TTCCTCGGCG CTCAAGCGCT TCCTGCGCGA CCTGCCTGAT 
GGGCTCTTCA CTCGCGCCCA GCGCCTAACC TGGCTGGAGG CCTCAGAGAT TGAGGACGAG 
GAGGAGAAGG TCTCCAGGTA CCGAGAGCTG CTGGTGCGGC TGCCCCCTGT CAACCGGGCC 
ACAGTGAAGG CCCTTATCAG CCACCTGTAC TGTGTTCAGT GCTTCTCAGA CACGAACCAG 
ATGAACGTGC ACAACCTGGC AATTGTGTTT GGGCCCACGC TCTTCCAGAC AGATGGGCAG 
GACTACAAGG CTGGCCGTGT GGTGGAAGAC CTCATTAACC ACTATGTGGT GGTGTTTAGT 
GTGGATGAGG AAGAGCTCAG GAAGCAGCGG GAGGAGATCA CTGCCATTGT GAAGATGCGC 
GTGGCTGGCA CTGCCAGTGG GACCCAGCAT GCCGGTGACT TCATCTGCAC AGTGTATCTG 
GAAGAGAAGA AGGCAGAGAC TGAGCAGCAT ATCAAGGTCC CAGCATCCAT GACTGCTGAG 
GAGCTCACCC TGGAGATCCT GGATCGCCGG AACGTGGGCA TCAGGGAGAA GGACTATTGG 
ACCTGCTTTG AGGTCAACGA GAGGGAGGAG GCAGAGCGCC CCCTGCACTT TGCGGAGAAG 
GTGCTGCCCA TCCTGCACGG GCTGGGCACG GACAGCCACC TGGTGGTGAA GAAGCACCAG 
GCCATGGAGG CCATGCTGCT GTACCTGGCC AGCCGTGTCG GTGACACCAA GCATGGCATG 
ATGAAGTTCC GTGAGGACCG CAGCCTCCTG GGCCTGGGCC TGCCCTCAGG TGGCTTCCAC 
GATCGCTACT TCATCCTCAA CAGCAGCTGC TTGCGGCTCT ACAAGGAGGT CCGGAGCCAG 
AGGCCGTGGA GCGGGGCCCC TGAGACCAGT CACCGGCCTG AGAAGGAGTG GCCTATTAAG 
AGTCTCAAAG TCTACCTGGG AGTGAAGAAG AAACTCAGGC CACCCACCTG CTGGGGCTTC 
ACAGTGGTGC ATGAGACAGA GAAACATGAG AAGCAGCAGT GGTACCTCTG CTGTGACACA 
CAGATGGAGC TCCGGGAGTG GTTCGCTACC TTTCTGTTTG TGCAGCATGA CGGCCTGGTG 
TGGCCCTCAG AGCCCTCACG CGTGTCCCGG GCAGTGCCTG AGGTCCGGCT GGGTAGTGTG 
TCACTGATCC CCCTTCGAGG TAGTGAAAAT GAAATGCGCC GGAGTGTGGC TGCCTTCACC 
GCGGACCCTC TGTCTCTTCT GCGCAACGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035207.1
CDS	190..4542
Translation	MAEAGDAALSVAEWLRALHLEQYTGLFEQHGLVWATECQGLSDTRLMDMGMLLPGHRRRILAGLLRAHTSPAPAPRPTPRPVPMKRHIFRSPPVPATPPEPLPTTTEDEGLPAAPPIPPRRSCLPPTCFTTPSTAAPDPVLPPLPAKRHLAELSVPPVPPRTGPPRLLVSLPTKEEESLLPSLSSPPQPQSEEPLSTLPQGPPQPPSPPPCPPEIPPKPVRLFPEFDDSDYDEVPEEGPGAPARVMTKKEEPPPSRVPRAVRVASLLSEGEELSGDDQGDEEEDDHAYEGVPNGGWHTSSLSLSLPSTIAAPHPMDGPPGGSTPVTPVIKAGWLDKNPPQGSYIYQKRWVRLDTDHLRYFDSNKDAYSKRFISVACISHVAAIGDQKFEVITNNRTFAFRAESDVERKEWMQALQQAMAEQRARARLSSAYLLGVPGSEQPDRAGSLELRGFKNKLYVAVVGDKVQLYKNLEEYHLGIGITFIDMSVGNVKEVDRRSFDLTTPYRIFSFSADSELEKEQWLEAMQGAIAEALSTSEVAERIWAAAPNRFCADCGAPQPDWASINLCVVICKRCAGEHRGLGAGVSKVRSLKMDRKVWTETLIELFLQLGNGAGNRFWAANVPPSEALQPSSSPSTRRCHLEAKYREGKYRRYHPLFGNQEELDKALCAAVTTTDLAETQALLGCGAGINCFSGDPEAPTPLALAEQAGQTLQMEFLRNNRTTEVPRLDSMKPLEKHYSVVLPTVSHSGFLYKTASAGKLLQDRRAREEFSRRWCVLGDGVLSYFENERAVTPNGEIRASEIVCLAVPPPDTHGFEHTFEVYTEGERLYLFGLESAEQAHEWVKCIAKAFVPPLAEDLLARDFERLGRLPYKAGLSLQRAQEGWFSLSGSELRAVFPEGPCEEPLQLRKLQELSIQGDSENQVLVLVERRRTLYIQGERRLDFMGWLGAIQKAAASMGDTLSEQQLGDSDIPVIVYRCVDYITQCGLTSEGIYRKCGQTSKTQRLLESLRQDARSVHLKEGEQHVDDVSSALKRFLRDLPDGLFTRAQRLTWLEASEIEDEEEKVSRYRELLVRLPPVNRATVKALISHLYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFSVDEEELRKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIREKDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSQRPWSGAPETSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRVSRAVPEVRLGSVSLIPLRGSENEMRRSVAAFTADPLSLLRNV

Target ORF information:

RefSeq Version	NM_001040118.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040118.2

ORF Insert Sequence:

ATGGCAGAGG CTGGGGATGC TGCGCTATCG GTGGCCGAGT GGCTGCGGGC ATTGCACCTG 
GAGCAGTACA CGGGGCTCTT TGAGCAGCAT GGCCTGGTGT GGGCCACTGA GTGCCAAGGC 
CTCAGCGACA CCCGCCTGAT GGACATGGGC ATGCTACTCC CTGGTCACCG CCGCCGCATC 
CTGGCTGGCC TGCTCCGTGC CCATACCTCA CCGGCCCCTG CACCCCGCCC CACCCCACGG 
CCTGTGCCCA TGAAGCGCCA CATCTTCCGC TCACCACCTG TGCCTGCCAC TCCACCCGAG 
CCGCTGCCCA CCACTACAGA GGATGAGGGG CTCCCCGCTG CCCCACCCAT CCCGCCCCGG 
AGGAGCTGCC TTCCGCCCAC CTGCTTCACC ACCCCATCCA CAGCTGCCCC AGACCCTGTG 
CTGCCCCCGC TGCCTGCTAA GCGGCATTTG GCAGAGCTGA GCGTTCCACC CGTGCCGCCC 
CGCACCGGAC CCCCCCGCCT GCTGGTGAGC CTGCCCACTA AGGAGGAGGA GTCATTGCTG 
CCATCATTAT CATCCCCTCC CCAGCCACAG TCTGAGGAGC CCCTGTCCAC CCTCCCCCAG 
GGGCCTCCCC AGCCTCCCTC TCCACCTCCC TGCCCCCCGG AGATACCTCC AAAGCCGGTA 
CGCCTGTTCC CAGAGTTCGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGGGG 
GCCCCAGCCA GAGTGATGAC CAAGAAGGAG GAGCCCCCAC CGAGCCGAGT CCCACGGGCC 
GTGCGCGTGG CCAGTCTGCT GAGCGAGGGA GAGGAACTGT CTGGGGACGA CCAAGGGGAT 
GAGGAAGAGG ATGACCACGC CTATGAGGGC GTCCCCAATG GCGGATGGCA TACCAGCAGC 
CTGAGCTTGT CCTTGCCCAG CACAATAGCT GCGCCACACC CCATGGACGG GCCGCCTGGG 
GGCTCCACCC CCGTCACACC AGTCATCAAG GCTGGCTGGC TGGACAAGAA CCCACCGCAG 
GGATCTTACA TCTATCAGAA ACGATGGGTG AGACTGGATA CTGATCACCT GCGATACTTT 
GACAGTAACA AGGACGCTTA CTCTAAGCGC TTTATCTCTG TGGCCTGCAT CTCCCACGTG 
GCTGCCATCG GGGACCAGAA GTTTGAAGTG ATCACAAACA ACCGAACCTT TGCCTTCCGG 
GCAGAGAGTG ATGTGGAGCG GAAGGAGTGG ATGCAGGCCC TGCAGCAGGC CATGGCTGAG 
CAGCGTGCCC GGGCCCGGCT CTCTAGCGCT TATCTGCTGG GAGTTCCAGG CTCAGAGCAG 
CCTGACCGCG CTGGCAGCCT GGAGCTTCGT GGCTTCAAGA ATAAGCTGTA CGTGGCCGTG 
GTCGGGGACA AAGTGCAGCT CTACAAGAAT CTAGAGGAGT ACCACCTGGG CATTGGCATC 
ACCTTCATCG ACATGAGCGT GGGCAACGTG AAGGAAGTGG ACCGGCGCAG CTTCGACCTC 
ACCACGCCCT ACCGCATCTT CAGCTTCTCT GCTGACTCAG AGCTAGAGAA GGAGCAGTGG 
TTGGAGGCCA TGCAGGGAGC CATCGCTGAG GCCCTGTCTA CCTCGGAGGT GGCCGAGCGC 
ATCTGGGCTG CAGCCCCCAA CAGGTTCTGT GCTGACTGCG GGGCTCCTCA GCCTGACTGG 
GCCTCCATCA ACCTCTGTGT TGTTATCTGC AAGCGCTGTG CAGGGGAGCA CCGTGGCCTG 
GGCGCTGGCG TCTCCAAGGT GCGGAGCCTG AAGATGGACA GGAAGGTGTG GACAGAAACA 
CTTATCGAGC TCTTCTTACA GCTGGGGAAT GGCGCTGGGA ACCGCTTCTG GGCAGCCAAC 
GTGCCCCCCA GTGAGGCCCT GCAGCCCAGC AGCAGCCCCA GCACCCGGCG GTGCCACCTG 
GAGGCCAAGT ACCGTGAGGG CAAGTACCGC CGCTACCACC CGCTCTTTGG CAACCAGGAG 
GAGCTGGACA AGGCCCTGTG TGCTGCAGTC ACCACCACAG ACCTGGCTGA GACCCAGGCG 
CTCCTGGGCT GTGGGGCTGG GATCAACTGC TTCTCGGGGG ACCCTGAGGC CCCCACGCCC 
CTGGCTCTTG CAGAGCAGGC GGGGCAGACG CTGCAGATGG AATTCCTTCG GAACAACCGG 
ACCACAGAGG TGCCTCGGCT GGACTCGATG AAGCCCCTGG AAAAGCACTA CTCAGTTGTC 
CTGCCGACCG TGAGCCACAG TGGCTTCCTC TACAAGACTG CCTCTGCCGG CAAGCTGCTA 
CAGGACCGCC GGGCCCGGGA AGAGTTCAGC CGGCGCTGGT GTGTCCTTGG TGACGGGGTC 
CTGAGCTACT TTGAGAATGA GCGGGCAGTG ACCCCCAATG GAGAGATTCG GGCCAGCGAG 
ATTGTGTGCC TGGCAGTGCC CCCTCCTGAC ACCCATGGCT TTGAGCACAC CTTTGAGGTG 
TACACGGAGG GAGAACGGCT GTACCTGTTT GGGCTGGAGA GTGCGGAGCA GGCTCATGAG 
TGGGTCAAGT GTATTGCTAA GGCATTCGTG CCTCCCCTAG CCGAGGATCT GCTGGCCCGG 
GATTTTGAGC GGCTGGGACG CCTACCCTAC AAAGCTGGCC TGAGCCTACA GCGGGCCCAG 
GAGGGCTGGT TCTCTCTCAG TGGCTCGGAG CTCCGTGCTG TCTTCCCGGA GGGGCCCTGC 
GAAGAGCCGC TGCAACTACG GAAACTGCAG GAGCTTTCCA TCCAGGGGGA CAGTGAGAAC 
CAGGTGCTGG TGCTGGTGGA GCGAAGGAGG ACACTGTACA TACAGGGCGA GCGGCGGCTG 
GACTTCATGG GTTGGCTGGG GGCCATCCAG AAAGCAGCCG CCAGCATGGG GGACACGCTG 
TCGGAGCAGC AGCTTGGGGA CTCGGATATC CCGGTGATCG TGTACCGCTG TGTGGACTAC 
ATCACGCAGT GCGGCCTGAC CTCCGAGGGC ATCTACCGCA AGTGTGGGCA GACATCGAAG 
ACACAGCGGC TGCTGGAGAG CCTGCGGCAG GATGCGCGCT CTGTGCACCT CAAGGAGGGC 
GAGCAGCACG TGGATGATGT TTCCTCGGCG CTCAAGCGCT TCCTGCGCGA CCTGCCTGAT 
GGGCTCTTCA CTCGCGCCCA GCGCCTAACC TGGCTGGAGG CCTCAGAGAT TGAGGACGAG 
GAGGAGAAGG TCTCCAGGTA CCGAGAGCTG CTGGTGCGGC TGCCCCCTGT CAACCGGGCC 
ACAGTGAAGG CCCTTATCAG CCACCTGTAC TGTGTTCAGT GCTTCTCAGA CACGAACCAG 
ATGAACGTGC ACAACCTGGC AATTGTGTTT GGGCCCACGC TCTTCCAGAC AGATGGGCAG 
GACTACAAGG CTGGCCGTGT GGTGGAAGAC CTCATTAACC ACTATGTGGT GGTGTTTAGT 
GTGGATGAGG AAGAGCTCAG GAAGCAGCGG GAGGAGATCA CTGCCATTGT GAAGATGCGC 
GTGGCTGGCA CTGCCAGTGG GACCCAGCAT GCCGGTGACT TCATCTGCAC AGTGTATCTG 
GAAGAGAAGA AGGCAGAGAC TGAGCAGCAT ATCAAGGTCC CAGCATCCAT GACTGCTGAG 
GAGCTCACCC TGGAGATCCT GGATCGCCGG AACGTGGGCA TCAGGGAGAA GGACTATTGG 
ACCTGCTTTG AGGTCAACGA GAGGGAGGAG GCAGAGCGCC CCCTGCACTT TGCGGAGAAG 
GTGCTGCCCA TCCTGCACGG GCTGGGCACG GACAGCCACC TGGTGGTGAA GAAGCACCAG 
GCCATGGAGG CCATGCTGCT GTACCTGGCC AGCCGTGTCG GTGACACCAA GCATGGCATG 
ATGAAGTTCC GTGAGGACCG CAGCCTCCTG GGCCTGGGCC TGCCCTCAGG TGGCTTCCAC 
GATCGCTACT TCATCCTCAA CAGCAGCTGC TTGCGGCTCT ACAAGGAGGT CCGGAGCCAG 
AGGCCGTGGA GCGGGGCCCC TGAGACCAGT CACCGGCCTG AGAAGGAGTG GCCTATTAAG 
AGTCTCAAAG TCTACCTGGG AGTGAAGAAG AAACTCAGGC CACCCACCTG CTGGGGCTTC 
ACAGTGGTGC ATGAGACAGA GAAACATGAG AAGCAGCAGT GGTACCTCTG CTGTGACACA 
CAGATGGAGC TCCGGGAGTG GTTCGCTACC TTTCTGTTTG TGCAGCATGA CGGCCTGGTG 
TGGCCCTCAG AGCCCTCACG CGTGTCCCGG GCAGTGCCTG AGGTCCGGCT GGGTAGTGTG 
TCACTGATCC CCCTTCGAGG TAGTGAAAAT GAAATGCGCC GGAGTGTGGC TGCCTTCACC 
GCGGACCCTC TGTCTCTTCT GCGCAACGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Decreased STARD10 Expression Is Associated with Defective Insulin Secretion in Humans and Mice.
American journal of human genetics100(2)238-256(2017 Feb)
Carrat GR,Hu M,Nguyen-Tu MS,Chabosseau P,Gaulton KJ,van de Bunt M,Siddiq A,Falchi M,Thurner M,Canouil M,Pattou F,Leclerc I,Pullen TJ,Cane MC,Prabhala P,Greenwald W,Schulte A,Marchetti P,Ibberson M,MacDonald PE,Manning Fox JE,Gloyn AL,Froguel P,Solimena M,McCarthy MI,Rutter GA

Joint effect of CENTD2 and KCNQ1 polymorphisms on the risk of type 2 diabetes mellitus among Chinese Han population.
Molecular and cellular endocrinology40746-51(2015 May)
Qian Y,Dong M,Lu F,Li H,Jin G,Hu Z,Shen C,Shen H

Genome-wide trans-ancestry meta-analysis provides insight into the genetic architecture of type 2 diabetes susceptibility.
Nature genetics46(3)234-44(2014 Mar)
,,,,,Mahajan A,Go MJ,Zhang W,Below JE,Gaulton KJ,Ferreira T,Horikoshi M,Johnson AD,Ng MC,Prokopenko I,Saleheen D,Wang X,Zeggini E,Abecasis GR,Adair LS,Almgren P,Atalay M,Aung T,Baldassarre D,Balkau B,Bao Y,Barnett AH,Barroso I,Basit A,Been LF,Beilby J,Bell GI,Benediktsson R,Bergman RN,Boehm BO,Boerwinkle E,Bonnycastle LL,Burtt N,Cai Q,Campbell H,Carey J,Cauchi S,Caulfield M,Chan JC,Chang LC,Chang TJ,Chang YC,Charpentier G,Chen CH,Chen H,Chen YT,Chia KS,Chidambaram M,Chines PS,Cho NH,Cho YM,Chuang LM,Collins FS,Cornelis MC,Couper DJ,Crenshaw AT,van Dam RM,Danesh J,Das D,de Faire U,Dedoussis G,Deloukas P,Dimas AS,Dina C,Doney AS,Donnelly PJ,Dorkhan M,van Duijn C,Dupuis J,Edkins S,Elliott P,Emilsson V,Erbel R,Eriksson JG,Escobedo J,Esko T,Eury E,Florez JC,Fontanillas P,Forouhi NG,Forsen T,Fox C,Fraser RM,Frayling TM,Froguel P,Frossard P,Gao Y,Gertow K,Gieger C,Gigante B,Grallert H,Grant GB,Grrop LC,Groves CJ,Grundberg E,Guiducci C,Hamsten A,Han BG,Hara K,Hassanali N,Hattersley AT,Hayward C,Hedman AK,Herder C,Hofman A,Holmen OL,Hovingh K,Hreidarsson AB,Hu C,Hu FB,Hui J,Humphries SE,Hunt SE,Hunter DJ,Hveem K,Hydrie ZI,Ikegami H,Illig T,Ingelsson E,Islam M,Isomaa B,Jackson AU,Jafar T,James A,Jia W,J?ckel KH,Jonsson A,Jowett JB,Kadowaki T,Kang HM,Kanoni S,Kao WH,Kathiresan S,Kato N,Katulanda P,Keinanen-Kiukaanniemi KM,Kelly AM,Khan H,Khaw KT,Khor CC,Kim HL,Kim S,Kim YJ,Kinnunen L,Klopp N,Kong A,Korpi-Hy?v?lti E,Kowlessur S,Kraft P,Kravic J,Kristensen MM,Krithika S,Kumar A,Kumate J,Kuusisto J,Kwak SH,Laakso M,Lagou V,Lakka TA,Langenberg C,Langford C,Lawrence R,Leander K,Lee JM,Lee NR,Li M,Li X,Li Y,Liang J,Liju S,Lim WY,Lind L,Lindgren CM,Lindholm E,Liu CT,Liu JJ,Lobbens S,Long J,Loos RJ,Lu W,Luan J,Lyssenko V,Ma RC,Maeda S,M?gi R,M?nnisto S,Matthews DR,Meigs JB,Melander O,Metspalu A,Meyer J,Mirza G,Mihailov E,Moebus S,Mohan V,Mohlke KL,Morris AD,M?hleisen TW,M?ller-Nurasyid M,Musk B,Nakamura J,Nakashima E,Navarro P,Ng PK,Nica AC,Nilsson PM,Nj?lstad I,N?then MM,Ohnaka K,Ong TH,Owen KR,Palmer CN,Pankow JS,Park KS,Parkin M,Pechlivanis S,Pedersen NL,Peltonen L,Perry JR,Peters A,Pinidiyapathirage JM,Platou CG,Potter S,Price JF,Qi L,Radha V,Rallidis L,Rasheed A,Rathman W,Rauramaa R,Raychaudhuri S,Rayner NW,Rees SD,Rehnberg E,Ripatti S,Robertson N,Roden M,Rossin EJ,Rudan I,Rybin D,Saaristo TE,Salomaa V,Saltevo J,Samuel M,Sanghera DK,Saramies J,Scott J,Scott LJ,Scott RA,Segr? AV,Sehmi J,Sennblad B,Shah N,Shah S,Shera AS,Shu XO,Shuldiner AR,Sigur?sson G,Sijbrands E,Silveira A,Sim X,Sivapalaratnam S,Small KS,So WY,Stan??kov? A,Stefansson K,Steinbach G,Steinthorsdottir V,Stirrups K,Strawbridge RJ,Stringham HM,Sun Q,Suo C,Syv?nen AC,Takayanagi R,Takeuchi F,Tay WT,Teslovich TM,Thorand B,Thorleifsson G,Thorsteinsdottir U,Tikkanen E,Trakalo J,Tremoli E,Trip MD,Tsai FJ,Tuomi T,Tuomilehto J,Uitterlinden AG,Valladares-Salgado A,Vedantam S,Veglia F,Voight BF,Wang C,Wareham NJ,Wennauer R,Wickremasinghe AR,Wilsgaard T,Wilson JF,Wiltshire S,Winckler W,Wong TY,Wood AR,Wu JY,Wu Y,Yamamoto K,Yamauchi T,Yang M,Yengo L,Yokota M,Young R,Zabaneh D,Zhang F,Zhang R,Zheng W,Zimmet PZ,Altshuler D,Bowden DW,Cho YS,Cox NJ,Cruz M,Hanis CL,Kooner J,Lee JY,Seielstad M,Teo YY,Boehnke M,Parra EJ,Chambers JC,Tai ES,McCarthy MI,Morris AP

A common functional regulatory variant at a type 2 diabetes locus upregulates ARAP1 expression in the pancreatic beta cell.
American journal of human genetics94(2)186-97(2014 Feb)
Kulzer JR,Stitzel ML,Morken MA,Huyghe JR,Fuchsberger C,Kuusisto J,Laakso M,Boehnke M,Collins FS,Mohlke KL

A genome-wide approach accounting for body mass index identifies genetic variants influencing fasting glycemic traits and insulin resistance.
Nature genetics44(6)659-69(2012 May)
Manning AK,Hivert MF,Scott RA,Grimsby JL,Bouatia-Naji N,Chen H,Rybin D,Liu CT,Bielak LF,Prokopenko I,Amin N,Barnes D,Cadby G,Hottenga JJ,Ingelsson E,Jackson AU,Johnson T,Kanoni S,Ladenvall C,Lagou V,Lahti J,Lecoeur C,Liu Y,Martinez-Larrad MT,Montasser ME,Navarro P,Perry JR,Rasmussen-Torvik LJ,Salo P,Sattar N,Shungin D,Strawbridge RJ,Tanaka T,van Duijn CM,An P,de Andrade M,Andrews JS,Aspelund T,Atalay M,Aulchenko Y,Balkau B,Bandinelli S,Beckmann JS,Beilby JP,Bellis C,Bergman RN,Blangero J,Boban M,Boehnke M,Boerwinkle E,Bonnycastle LL,Boomsma DI,Borecki IB,B?ttcher Y,Bouchard C,Brunner E,Budimir D,Campbell H,Carlson O,Chines PS,Clarke R,Collins FS,Corbat?n-Anchuelo A,Couper D,de Faire U,Dedoussis GV,Deloukas P,Dimitriou M,Egan JM,Eiriksdottir G,Erdos MR,Eriksson JG,Eury E,Ferrucci L,Ford I,Forouhi NG,Fox CS,Franzosi MG,Franks PW,Frayling TM,Froguel P,Galan P,de Geus E,Gigante B,Glazer NL,Goel A,Groop L,Gudnason V,Hallmans G,Hamsten A,Hansson O,Harris TB,Hayward C,Heath S,Hercberg S,Hicks AA,Hingorani A,Hofman A,Hui J,Hung J,Jarvelin MR,Jhun MA,Johnson PC,Jukema JW,Jula A,Kao WH,Kaprio J,Kardia SL,Keinanen-Kiukaanniemi S,Kivimaki M,Kolcic I,Kovacs P,Kumari M,Kuusisto J,Kyvik KO,Laakso M,Lakka T,Lannfelt L,Lathrop GM,Launer LJ,Leander K,Li G,Lind L,Lindstrom J,Lobbens S,Loos RJ,Luan J,Lyssenko V,M?gi R,Magnusson PK,Marmot M,Meneton P,Mohlke KL,Mooser V,Morken MA,Miljkovic I,Narisu N,O'Connell J,Ong KK,Oostra BA,Palmer LJ,Palotie A,Pankow JS,Peden JF,Pedersen NL,Pehlic M,Peltonen L,Penninx B,Pericic M,Perola M,Perusse L,Peyser PA,Polasek O,Pramstaller PP,Province MA,R?ikk?nen K,Rauramaa R,Rehnberg E,Rice K,Rotter JI,Rudan I,Ruokonen A,Saaristo T,Sabater-Lleal M,Salomaa V,Savage DB,Saxena R,Schwarz P,Seedorf U,Sennblad B,Serrano-Rios M,Shuldiner AR,Sijbrands EJ,Siscovick DS,Smit JH,Small KS,Smith NL,Smith AV,Stan??kov? A,Stirrups K,Stumvoll M,Sun YV,Swift AJ,T?njes A,Tuomilehto J,Trompet S,Uitterlinden AG,Uusitupa M,Vikstr?m M,Vitart V,Vohl MC,Voight BF,Vollenweider P,Waeber G,Waterworth DM,Watkins H,Wheeler E,Widen E,Wild SH,Willems SM,Willemsen G,Wilson JF,Witteman JC,Wright AF,Yaghootkar H,Zelenika D,Zemunik T,Zgaga L,,,Wareham NJ,McCarthy MI,Barroso I,Watanabe RM,Florez JC,Dupuis J,Meigs JB,Langenberg C

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ARAP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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