MRPL53 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPL53
Entrez Gene ID	116540
Full Name	mitochondrial ribosomal protein L53
Synonyms	L53MT
General protein information	Preferred Names mitochondrial ribosomal protein L53 Names 39S ribosomal protein L53, mitochondrial mitochondrial large ribosomal subunit protein mL53
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p13.1
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 1p. [provided by RefSeq, Jul 2008].
Disorder MIM:	611857

mRNA and Protein(s)

mRNA	Protein	Name
NM_053050.5	NP_444278.1	39S ribosomal protein L53, mitochondrial
NM_053050.4	NP_444278.1	39S ribosomal protein L53, mitochondrial

Pathways from BioSystems

Homologies

	MRPL53	XP_001110017.1
Canis lupus familiaris (dog)	MRPL53	XP_855093.1
Danio rerio (zebrafish)	zgc:103761	NP_001004653.1
Rattus norvegicus (Norway rat)	Mrpl53	NP_001102105.1
Gallus gallus (chicken)	MRPL53	NP_001186402.1
Danio rerio (zebrafish)	LOC100536741	XP_003200661.1
Homo sapiens (human)	MRPL53	NP_444278.1
Pan troglodytes (chimpanzee)	MRPL53	XP_515559.1
Xenopus tropicalis (tropical clawed frog)	mrpl53	NP_001005117.1
Bos taurus (cattle)	MRPL53	NP_001039391.1
Mus musculus (house mouse)	Mrpl53	NP_081020.1

Gene Ontology
Bibliography

Related articles in PubMed

MRPL53, a New Candidate Gene for Orofacial Clefting, Identified Using an eQTL Approach.
Masotti C, Brito LA, Nica AC, Ludwig KU, Nunes K, Savastano CP, Malcher C, Ferreira SG, Kobayashi GS, Bueno DF, Alonso N, Franco D, Rojas-Martinez A, Dos Santos SE, Galante PA, Meyer D, H?nemeier T, Mangold E, Dermitzakis ET, Passos-Bueno MR
Journal of dental research97(1)33-40(2018 Jan)

Structures of the human mitochondrial ribosome in native states of assembly.
Brown A, Rathore S, Kimanius D, Aibara S, Bai XC, Rorbach J, Amunts A, Ramakrishnan V
Nature structural & molecular biology24(10)866-869(2017 Oct)

Structure of the large ribosomal subunit from human mitochondria.
Brown A, Amunts A, Bai XC, Sugimoto Y, Edwards PC, Murshudov G, Scheres SHW, Ramakrishnan V
Science (New York, N.Y.)346(6210)718-722(2014 Nov)

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Zhang Z, Gerstein M
Genomics81(5)468-80(2003 May)

The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present.
Koc EC, Burkhart W, Blackburn K, Moyer MB, Schlatzer DM, Moseley A, Spremulli LL
The Journal of biological chemistry276(47)43958-69(2001 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

candidate gene for orofacial clefting
Title: MRPL53, a New Candidate Gene for Orofacial Clefting, Identified Using an eQTL Approach.

The following MRPL53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPL53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01529	NM_053050.5 Latest version!	Homo sapiens mitochondrial ribosomal protein L53 (MRPL53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu01529	NM_053050.4	Homo sapiens mitochondrial ribosomal protein L53 (MRPL53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPL53 ( NM_053050.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_444278.1 Homo sapiens mitochondrial ribosomal protein L53 (MRPL53), mRNA.

Price & Availability↑

CloneID	OHu01529
Clone ID Related Accession (Same CDS sequence)	NM_053050.5 , NM_053050.4
Accession Version	NM_053050.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	39S ribosomal protein L53, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ014848.1 and BC012163.1. On Nov 23, 2018 this sequence version replaced NM_053050.4. ##Evidence-Data-START## Transcript exon combination :: BP205925.1, ERR279839.1681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000258105.8/ ENSP00000258105.7 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGCTG CCTTGGCTCG GCTTGGTCTG CGGCCTGTCA AACAGGTTCG GGTTCAGTTC 
TGTCCCTTCG AGAAAAACGT GGAATCGACG AGGACCTTCC TGCAGACGGT GAGCAGTGAG 
AAGGTCCGCT CCACTAATCT CAACTGCTCA GTGATTGCGG ACGTGAGGCA TGACGGCTCC 
GAGCCCTGCG TGGACGTGCT GTTCGGAGAC GGGCATCGCC TGATTATGCG CGGCGCTCAT 
CTCACCGCTC TGGAAATGCT CACCGCCTTC GCCTCCCACA TCCGGGCCAG GGACGCGGCG 
GGCAGCGGGG ACAAGCCGGG CGCTGATACT GGTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444278.1
CDS	21..359
Translation	MAAALARLGLRPVKQVRVQFCPFEKNVESTRTFLQTVSSEKVRSTNLNCSVIADVRHDGSEPCVDVLFGDGHRLIMRGAHLTALEMLTAFASHIRARDAAGSGDKPGADTGR

Target ORF information:

RefSeq Version	NM_053050.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L53 (MRPL53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053050.5

ORF Insert Sequence:

ATGGCAGCTG CCTTGGCTCG GCTTGGTCTG CGGCCTGTCA AACAGGTTCG GGTTCAGTTC 
TGTCCCTTCG AGAAAAACGT GGAATCGACG AGGACCTTCC TGCAGACGGT GAGCAGTGAG 
AAGGTCCGCT CCACTAATCT CAACTGCTCA GTGATTGCGG ACGTGAGGCA TGACGGCTCC 
GAGCCCTGCG TGGACGTGCT GTTCGGAGAC GGGCATCGCC TGATTATGCG CGGCGCTCAT 
CTCACCGCTC TGGAAATGCT CACCGCCTTC GCCTCCCACA TCCGGGCCAG GGACGCGGCG 
GGCAGCGGGG ACAAGCCGGG CGCTGATACT GGTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL53 ( NM_053050.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_444278.1 Homo sapiens mitochondrial ribosomal protein L53 (MRPL53), mRNA.

Price & Availability↑

CloneID	OHu01529
Clone ID Related Accession (Same CDS sequence)	NM_053050.5 , NM_053050.4
Accession Version	NM_053050.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	39S ribosomal protein L53, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005041.2, BQ014848.1, BC012163.1 and BQ688110.1. On Feb 11, 2011 this sequence version replaced NM_053050.3. ##Evidence-Data-START## Transcript exon combination :: BQ014848.1, ERR279839.1681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCTG CCTTGGCTCG GCTTGGTCTG CGGCCTGTCA AACAGGTTCG GGTTCAGTTC 
TGTCCCTTCG AGAAAAACGT GGAATCGACG AGGACCTTCC TGCAGACGGT GAGCAGTGAG 
AAGGTCCGCT CCACTAATCT CAACTGCTCA GTGATTGCGG ACGTGAGGCA TGACGGCTCC 
GAGCCCTGCG TGGACGTGCT GTTCGGAGAC GGGCATCGCC TGATTATGCG CGGCGCTCAT 
CTCACCGCTC TGGAAATGCT CACCGCCTTC GCCTCCCACA TCCGGGCCAG GGACGCGGCG 
GGCAGCGGGG ACAAGCCGGG CGCTGATACT GGTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444278.1
CDS	156..494
Translation	MAAALARLGLRPVKQVRVQFCPFEKNVESTRTFLQTVSSEKVRSTNLNCSVIADVRHDGSEPCVDVLFGDGHRLIMRGAHLTALEMLTAFASHIRARDAAGSGDKPGADTGR

Target ORF information:

RefSeq Version	NM_053050.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L53 (MRPL53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053050.4

ORF Insert Sequence:

ATGGCAGCTG CCTTGGCTCG GCTTGGTCTG CGGCCTGTCA AACAGGTTCG GGTTCAGTTC 
TGTCCCTTCG AGAAAAACGT GGAATCGACG AGGACCTTCC TGCAGACGGT GAGCAGTGAG 
AAGGTCCGCT CCACTAATCT CAACTGCTCA GTGATTGCGG ACGTGAGGCA TGACGGCTCC 
GAGCCCTGCG TGGACGTGCT GTTCGGAGAC GGGCATCGCC TGATTATGCG CGGCGCTCAT 
CTCACCGCTC TGGAAATGCT CACCGCCTTC GCCTCCCACA TCCGGGCCAG GGACGCGGCG 
GGCAGCGGGG ACAAGCCGGG CGCTGATACT GGTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MRPL53, a New Candidate Gene for Orofacial Clefting, Identified Using an eQTL Approach.
Journal of dental research97(1)33-40(2018 Jan)
Masotti C,Brito LA,Nica AC,Ludwig KU,Nunes K,Savastano CP,Malcher C,Ferreira SG,Kobayashi GS,Bueno DF,Alonso N,Franco D,Rojas-Martinez A,Dos Santos SE,Galante PA,Meyer D,H?nemeier T,Mangold E,Dermitzakis ET,Passos-Bueno MR

Structures of the human mitochondrial ribosome in native states of assembly.
Nature structural & molecular biology24(10)866-869(2017 Oct)
Brown A,Rathore S,Kimanius D,Aibara S,Bai XC,Rorbach J,Amunts A,Ramakrishnan V

Structure of the large ribosomal subunit from human mitochondria.
Science (New York, N.Y.)346(6210)718-722(2014 Nov)
Brown A,Amunts A,Bai XC,Sugimoto Y,Edwards PC,Murshudov G,Scheres SHW,Ramakrishnan V

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Genomics81(5)468-80(2003 May)
Zhang Z,Gerstein M

The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present.
The Journal of biological chemistry276(47)43958-69(2001 Nov)
Koc EC,Burkhart W,Blackburn K,Moyer MB,Schlatzer DM,Moseley A,Spremulli LL

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MRPL53 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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