ARHGEF25 cDNA ORF clone, Homo sapiens(human)

The following ARHGEF25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu07857 NM_182947.3
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 25 (ARHGEF25), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu11079 NM_001111270.2
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 25 (ARHGEF25), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $363.30
$519.00
OHu100811 NM_001347933.1
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 25 (ARHGEF25), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu107246 NM_182947.4
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 25 (ARHGEF25), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu108207 NM_001111270.3
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 25 (ARHGEF25), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu108220 NM_001347933.2
Latest version!
Homo sapiens Rho guanine nucleotide exchange factor 25 (ARHGEF25), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu07857
    Clone ID Related Accession (Same CDS sequence) NM_182947.3
    Accession Version NM_182947.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-27
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 25 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF487514.2, AC025165.27 and AI677902.1. On Feb 1, 2012 this sequence version replaced NM_182947.2. Transcript Variant: This variant (1) represents the longest transcript (1) and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047559.1, AF487514.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCGGGGGG GGCACAAAGG GGGTCGCTGT GCCTGTCCCC GTGTGATCCG AAAAGTGCTG 
    GCAAAATGCG GCTGCTGCTT CGCCCGGGGG GGACGTGAAT CCTATTCCAT TGCGGGCAGT
    GAGGGGAGTA TATCGGCTTC TGCTGCCTCC GGTCTGGCTG CCCCCTCTGG CCCCAGCTCT
    GGCCTCAGCT CTGGCCCCTG TTCCCCAGGC CCCCCAGGGC CCGTCAGTGG CCTGAGGAGA
    TGGTTGGATC ATTCCAAACA TTGTCTCAGT GTGGAAACTG AGGCAGACAG TGGTCAGGCA
    GGACCATATG AGAACTGGAT GTTGGAGCCA GCTCTAGCCA CAGGAGAGGA GCTGCCGGAA
    CTGACCTTGC TGACCACACT GTTGGAGGGC CCTGGAGATA AGACGCAGCC ACCTGAAGAG
    GAGACTTTGT CCCAAGCCCC TGAGAGTGAG GAGGAACAGA AGAAGAAGGC TCTGGAAAGG
    AGTATGTATG TCCTGAGTGA ACTGGTAGAA ACAGAGAAAA TGTACGTGGA CGACTTGGGG
    CAGATTGTGG AGGGTTATAT GGCCACCATG GCTGCTCAGG GGGTCCCCGA GAGTCTTCGA
    GGCCGTGACA GGATTGTGTT TGGGAATATC CAGCAAATCT ATGAGTGGCA CCGAGACTAT
    TTCTTGCAAG AGCTACAACG GTGTCTGAAA GATCCTGATT GGCTGGCTCA GCTATTCATC
    AAACACGAGC GCCGGCTGCA TATGTATGTG GTGTACTGTC AGAATAAGCC CAAGTCAGAG
    CATGTGGTGT CAGAGTTTGG GGACAGCTAC TTTGAGGAGC TCCGGCAGCA GCTGGGGCAC
    CGCCTGCAGC TGAACGACCT CCTCATCAAA CCTGTGCAGC GGATCATGAA ATACCAGCTG
    CTGCTCAAGG ATTTTCTCAA GTATTACAAT AGAGCTGGGA TGGATACTGC AGACCTAGAG
    CAAGCTGTGG AGGTCATGTG CTTTGTGCCC AAGCGCTGCA ACGATATGAT GACGCTGGGG
    AGATTGCGGG GATTTGAGGG CAAACTGACT GCTCAGGGGA AGCTCTTGGG CCAGGACACT
    TTCTGGGTCA CCGAGCCTGA GGCTGGAGGG CTGCTGTCTT CCCGAGGTCG AGAGAGGCGC
    GTCTTCCTCT TTGAGCAAAT CATCATCTTC AGTGAAGCCC TGGGAGGAGG AGTGAGAGGT
    GGAACACAGC CTGGATATGT ATACAAGAAC AGCATTAAGG TGAGCTGCCT GGGACTGGAG
    GGGAACCTCC AAGGTGACCC TTGCCGCTTT GCACTGACCT CCAGAGGGCC AGAGGGTGGG
    ATCCAGCGCT ATGTCCTGCA GGCTGCAGAC CCTGCTATCA GTCAGGCCTG GATCAAGCAT
    GTGGCTCAGA TCTTGGAGAG CCAACGGGAC TTCCTCAACG CATTGCAGTC ACCCATTGAG
    TACCAGAGAC GGGAGAGCCA GACCAACAGC CTGGGGCGGC CAAGAGGGCC TGGAGTGGGG
    AGCCCTGGAA GAATTCGGCT TGGAGATCAG GCCCAGGGCA GCACACACAC ACCCATCAAT
    GGCTCTCTCC CCTCTCTGCT GCTGTCACCC AAAGGGGAGG TGGCCAGAGC CCTCTTGCCA
    CTGGATAAAC AGGCCCTTGG TGACATCCCC CAGGCTCCCC ATGACTCTCC TCCAGTCTCT
    CCAACTCCAA AAACCCCTCC CTGCCAAGCC AGACTTGCCA AGCTGGATGA AGATGAGCTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_891992.2
    CDS461..2203
    Translation

    Target ORF information:

    RefSeq Version NM_182947.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 25 (ARHGEF25), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_182947.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCGGGGGG GGCACAAAGG GGGTCGCTGT GCCTGTCCCC GTGTGATCCG AAAAGTGCTG 
    GCAAAATGCG GCTGCTGCTT CGCCCGGGGG GGACGTGAAT CCTATTCCAT TGCGGGCAGT
    GAGGGGAGTA TATCGGCTTC TGCTGCCTCC GGTCTGGCTG CCCCCTCTGG CCCCAGCTCT
    GGCCTCAGCT CTGGCCCCTG TTCCCCAGGC CCCCCAGGGC CCGTCAGTGG CCTGAGGAGA
    TGGTTGGATC ATTCCAAACA TTGTCTCAGT GTGGAAACTG AGGCAGACAG TGGTCAGGCA
    GGACCATATG AGAACTGGAT GTTGGAGCCA GCTCTAGCCA CAGGAGAGGA GCTGCCGGAA
    CTGACCTTGC TGACCACACT GTTGGAGGGC CCTGGAGATA AGACGCAGCC ACCTGAAGAG
    GAGACTTTGT CCCAAGCCCC TGAGAGTGAG GAGGAACAGA AGAAGAAGGC TCTGGAAAGG
    AGTATGTATG TCCTGAGTGA ACTGGTAGAA ACAGAGAAAA TGTACGTGGA CGACTTGGGG
    CAGATTGTGG AGGGTTATAT GGCCACCATG GCTGCTCAGG GGGTCCCCGA GAGTCTTCGA
    GGCCGTGACA GGATTGTGTT TGGGAATATC CAGCAAATCT ATGAGTGGCA CCGAGACTAT
    TTCTTGCAAG AGCTACAACG GTGTCTGAAA GATCCTGATT GGCTGGCTCA GCTATTCATC
    AAACACGAGC GCCGGCTGCA TATGTATGTG GTGTACTGTC AGAATAAGCC CAAGTCAGAG
    CATGTGGTGT CAGAGTTTGG GGACAGCTAC TTTGAGGAGC TCCGGCAGCA GCTGGGGCAC
    CGCCTGCAGC TGAACGACCT CCTCATCAAA CCTGTGCAGC GGATCATGAA ATACCAGCTG
    CTGCTCAAGG ATTTTCTCAA GTATTACAAT AGAGCTGGGA TGGATACTGC AGACCTAGAG
    CAAGCTGTGG AGGTCATGTG CTTTGTGCCC AAGCGCTGCA ACGATATGAT GACGCTGGGG
    AGATTGCGGG GATTTGAGGG CAAACTGACT GCTCAGGGGA AGCTCTTGGG CCAGGACACT
    TTCTGGGTCA CCGAGCCTGA GGCTGGAGGG CTGCTGTCTT CCCGAGGTCG AGAGAGGCGC
    GTCTTCCTCT TTGAGCAAAT CATCATCTTC AGTGAAGCCC TGGGAGGAGG AGTGAGAGGT
    GGAACACAGC CTGGATATGT ATACAAGAAC AGCATTAAGG TGAGCTGCCT GGGACTGGAG
    GGGAACCTCC AAGGTGACCC TTGCCGCTTT GCACTGACCT CCAGAGGGCC AGAGGGTGGG
    ATCCAGCGCT ATGTCCTGCA GGCTGCAGAC CCTGCTATCA GTCAGGCCTG GATCAAGCAT
    GTGGCTCAGA TCTTGGAGAG CCAACGGGAC TTCCTCAACG CATTGCAGTC ACCCATTGAG
    TACCAGAGAC GGGAGAGCCA GACCAACAGC CTGGGGCGGC CAAGAGGGCC TGGAGTGGGG
    AGCCCTGGAA GAATTCGGCT TGGAGATCAG GCCCAGGGCA GCACACACAC ACCCATCAAT
    GGCTCTCTCC CCTCTCTGCT GCTGTCACCC AAAGGGGAGG TGGCCAGAGC CCTCTTGCCA
    CTGGATAAAC AGGCCCTTGG TGACATCCCC CAGGCTCCCC ATGACTCTCC TCCAGTCTCT
    CCAACTCCAA AAACCCCTCC CTGCCAAGCC AGACTTGCCA AGCTGGATGA AGATGAGCTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11079
    Clone ID Related Accession (Same CDS sequence) NM_001111270.2
    Accession Version NM_001111270.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-24
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 25 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB370196.1, AC025165.27 and AI677902.1. This sequence is a reference standard in the RefSeqGene project. On Feb 1, 2012 this sequence version replaced NM_001111270.1. Transcript Variant: This variant (3) differs in the 5' UTR, and in the presence and absence of exons in the 5' coding region, compared to variant 1. The resulting isoform (3) contains a distinct N-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB370196.1, SRR1660803.224487.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAGCCCC CGGACCGCCC CGCCCCTGGC CGCACTGACC GGATACTGGG GGTCATGGGG 
    GGCATGCTGC GCGCATGCGC CCTCCCTGGG CAGGAGGGGC CCCCAAGGAG AAGCCCTCTA
    GGGTTGGTGG GTACCGAGCC AGAGTCTGAA CGTACGGAGG GAGATCACAG AAGGGATCGC
    GAACATGAGG TCCTCGCCGG GGCTCTGCAG CCCGAATCCT ATTCCATTGC GGGCAGTGAG
    GGGAGTATAT CGGCTTCTGC TGCCTCCGGT CTGGCTGCCC CCTCTGGCCC CAGCTCTGGC
    CTCAGCTCTG GCCCCTGTTC CCCAGGCCCC CCAGGGCCCG TCAGTGGCCT GAGGAGATGG
    TTGGATCATT CCAAACATTG TCTCAGTGTG GAAACTGAGG CAGACAGTGG TCAGGCAGGA
    CCATATGAGA ACTGGATGTT GGAGCCAGCT CTAGCCACAG GAGAGGAGCT GCCGGAACTG
    ACCTTGCTGA CCACACTGTT GGAGGGCCCT GGAGATAAGA CGCAGCCACC TGAAGAGGAG
    ACTTTGTCCC AAGCCCCTGA GAGTGAGGAG GAACAGAAGA AGAAGGCTCT GGAAAGGAGT
    ATGTATGTCC TGAGTGAACT GGTAGAAACA GAGAAAATGT ACGTGGACGA CTTGGGGCAG
    ATTGTGGAGG GTTATATGGC CACCATGGCT GCTCAGGGGG TCCCCGAGAG TCTTCGAGGC
    CGTGACAGGA TTGTGTTTGG GAATATCCAG CAAATCTATG AGTGGCACCG AGACTATTTC
    TTGCAAGAGC TACAACGGTG TCTGAAAGAT CCTGATTGGC TGGCTCAGCT ATTCATCAAA
    CACGAGCGCC GGCTGCATAT GTATGTGGTG TACTGTCAGA ATAAGCCCAA GTCAGAGCAT
    GTGGTGTCAG AGTTTGGGGA CAGCTACTTT GAGGAGCTCC GGCAGCAGCT GGGGCACCGC
    CTGCAGCTGA ACGACCTCCT CATCAAACCT GTGCAGCGGA TCATGAAATA CCAGCTGCTG
    CTCAAGGATT TTCTCAAGTA TTACAATAGA GCTGGGATGG ATACTGCAGA CCTAGAGCAA
    GCTGTGGAGG TCATGTGCTT TGTGCCCAAG CGCTGCAACG ATATGATGAC GCTGGGGAGA
    TTGCGGGGAT TTGAGGGCAA ACTGACTGCT CAGGGGAAGC TCTTGGGCCA GGACACTTTC
    TGGGTCACCG AGCCTGAGGC TGGAGGGCTG CTGTCTTCCC GAGGTCGAGA GAGGCGCGTC
    TTCCTCTTTG AGCAAATCAT CATCTTCAGT GAAGCCCTGG GAGGAGGAGT GAGAGGTGGA
    ACACAGCCTG GATATGTATA CAAGAACAGC ATTAAGGTGA GCTGCCTGGG ACTGGAGGGG
    AACCTCCAAG GTGACCCTTG CCGCTTTGCA CTGACCTCCA GAGGGCCAGA GGGTGGGATC
    CAGCGCTATG TCCTGCAGGC TGCAGACCCT GCTATCAGTC AGGCCTGGAT CAAGCATGTG
    GCTCAGATCT TGGAGAGCCA ACGGGACTTC CTCAACGCAT TGCAGTCACC CATTGAGTAC
    CAGAGACGGG AGAGCCAGAC CAACAGCCTG GGGCGGCCAA GAGGGCCTGG AGTGGGGAGC
    CCTGGAAGAA TTCGGCTTGG AGATCAGGCC CAGGGCAGCA CACACACACC CATCAATGGC
    TCTCTCCCCT CTCTGCTGCT GTCACCCAAA GGGGAGGTGG CCAGAGCCCT CTTGCCACTG
    GATAAACAGG CCCTTGGTGA CATCCCCCAG GCTCCCCATG ACTCTCCTCC AGTCTCTCCA
    ACTCCAAAAA CCCCTCCCTG CCAAGCCAGA CTTGCCAAGC TGGATGAAGA TGAGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001104740.1
    CDS38..1897
    Translation

    Target ORF information:

    RefSeq Version NM_001111270.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 25 (ARHGEF25), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001111270.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAGCCCC CGGACCGCCC CGCCCCTGGC CGCACTGACC GGATACTGGG GGTCATGGGG 
    GGCATGCTGC GCGCATGCGC CCTCCCTGGG CAGGAGGGGC CCCCAAGGAG AAGCCCTCTA
    GGGTTGGTGG GTACCGAGCC AGAGTCTGAA CGTACGGAGG GAGATCACAG AAGGGATCGC
    GAACATGAGG TCCTCGCCGG GGCTCTGCAG CCCGAATCCT ATTCCATTGC GGGCAGTGAG
    GGGAGTATAT CGGCTTCTGC TGCCTCCGGT CTGGCTGCCC CCTCTGGCCC CAGCTCTGGC
    CTCAGCTCTG GCCCCTGTTC CCCAGGCCCC CCAGGGCCCG TCAGTGGCCT GAGGAGATGG
    TTGGATCATT CCAAACATTG TCTCAGTGTG GAAACTGAGG CAGACAGTGG TCAGGCAGGA
    CCATATGAGA ACTGGATGTT GGAGCCAGCT CTAGCCACAG GAGAGGAGCT GCCGGAACTG
    ACCTTGCTGA CCACACTGTT GGAGGGCCCT GGAGATAAGA CGCAGCCACC TGAAGAGGAG
    ACTTTGTCCC AAGCCCCTGA GAGTGAGGAG GAACAGAAGA AGAAGGCTCT GGAAAGGAGT
    ATGTATGTCC TGAGTGAACT GGTAGAAACA GAGAAAATGT ACGTGGACGA CTTGGGGCAG
    ATTGTGGAGG GTTATATGGC CACCATGGCT GCTCAGGGGG TCCCCGAGAG TCTTCGAGGC
    CGTGACAGGA TTGTGTTTGG GAATATCCAG CAAATCTATG AGTGGCACCG AGACTATTTC
    TTGCAAGAGC TACAACGGTG TCTGAAAGAT CCTGATTGGC TGGCTCAGCT ATTCATCAAA
    CACGAGCGCC GGCTGCATAT GTATGTGGTG TACTGTCAGA ATAAGCCCAA GTCAGAGCAT
    GTGGTGTCAG AGTTTGGGGA CAGCTACTTT GAGGAGCTCC GGCAGCAGCT GGGGCACCGC
    CTGCAGCTGA ACGACCTCCT CATCAAACCT GTGCAGCGGA TCATGAAATA CCAGCTGCTG
    CTCAAGGATT TTCTCAAGTA TTACAATAGA GCTGGGATGG ATACTGCAGA CCTAGAGCAA
    GCTGTGGAGG TCATGTGCTT TGTGCCCAAG CGCTGCAACG ATATGATGAC GCTGGGGAGA
    TTGCGGGGAT TTGAGGGCAA ACTGACTGCT CAGGGGAAGC TCTTGGGCCA GGACACTTTC
    TGGGTCACCG AGCCTGAGGC TGGAGGGCTG CTGTCTTCCC GAGGTCGAGA GAGGCGCGTC
    TTCCTCTTTG AGCAAATCAT CATCTTCAGT GAAGCCCTGG GAGGAGGAGT GAGAGGTGGA
    ACACAGCCTG GATATGTATA CAAGAACAGC ATTAAGGTGA GCTGCCTGGG ACTGGAGGGG
    AACCTCCAAG GTGACCCTTG CCGCTTTGCA CTGACCTCCA GAGGGCCAGA GGGTGGGATC
    CAGCGCTATG TCCTGCAGGC TGCAGACCCT GCTATCAGTC AGGCCTGGAT CAAGCATGTG
    GCTCAGATCT TGGAGAGCCA ACGGGACTTC CTCAACGCAT TGCAGTCACC CATTGAGTAC
    CAGAGACGGG AGAGCCAGAC CAACAGCCTG GGGCGGCCAA GAGGGCCTGG AGTGGGGAGC
    CCTGGAAGAA TTCGGCTTGG AGATCAGGCC CAGGGCAGCA CACACACACC CATCAATGGC
    TCTCTCCCCT CTCTGCTGCT GTCACCCAAA GGGGAGGTGG CCAGAGCCCT CTTGCCACTG
    GATAAACAGG CCCTTGGTGA CATCCCCCAG GCTCCCCATG ACTCTCCTCC AGTCTCTCCA
    ACTCCAAAAA CCCCTCCCTG CCAAGCCAGA CTTGCCAAGC TGGATGAAGA TGAGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100811
    Clone ID Related Accession (Same CDS sequence) NM_001347933.1
    Accession Version NM_001347933.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1653bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 25 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025165.27 and BC012860.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.156503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCGGGGGG GGCACAAAGG GGGTCGCTGT GCCTGTCCCC GTGTGATCCG AAAAGTGCTG 
    GCAAAATGCG GCTGCTGCTT CGCCCGGGGG GGACGTGAAT CCTATTCCAT TGCGGGCAGT
    GAGGGGAGTA TATCGGCTTC TGCTGCCTCC GGTCTGGCTG CCCCCTCTGG CCCCAGCTCT
    GGCCTCAGCT CTGGCCCCTG TTCCCCAGGC CCCCCAGGGC CCGTCAGTGG CCTGAGGAGA
    TGGTTGGATC ATTCCAAACA TTGTCTCAGT GTGGAAACTG AGGCAGACAG TGGTCAGGCA
    GGACCATATG AGAACTGGAT GTTGGAGCCA GCTCTAGCCA CAGGAGAGGA GCTGCCGGAA
    CTGACCTTGC TGACCACACT GTTGGAGGGC CCTGGAGATA AGACGCAGCC ACCTGAAGAG
    GAGACTTTGT CCCAAGCCCC TGAGAGTGAG GAGGAACAGA AGAAGAAGGC TCTGGAAAGG
    AGTATGTATG TCCTGAGTGA ACTGGTAGAA ACAGAGAAAA TGTACGTGGA CGACTTGGGG
    CAGATTGTGG AGGGTTATAT GGCCACCATG GCTGCTCAGG GGGTCCCCGA GAGTCTTCGA
    GGCCGTGACA GGATTGTGTT TGGGAATATC CAGCAAATCT ATGAGTGGCA CCGAGACTAT
    TTCTTGCAAG AGCTACAACG GTGTCTGAAA GATCCTGATT GGCTGGCTCA GCTATTCATC
    AAACACGAGC TCCGGCAGCA GCTGGGGCAC CGCCTGCAGC TGAACGACCT CCTCATCAAA
    CCTGTGCAGC GGATCATGAA ATACCAGCTG CTGCTCAAGG ATTTTCTCAA GTATTACAAT
    AGAGCTGGGA TGGATACTGC AGACCTAGAG CAAGCTGTGG AGGTCATGTG CTTTGTGCCC
    AAGCGCTGCA ACGATATGAT GACGCTGGGG AGATTGCGGG GATTTGAGGG CAAACTGACT
    GCTCAGGGGA AGCTCTTGGG CCAGGACACT TTCTGGGTCA CCGAGCCTGA GGCTGGAGGG
    CTGCTGTCTT CCCGAGGTCG AGAGAGGCGC GTCTTCCTCT TTGAGCAAAT CATCATCTTC
    AGTGAAGCCC TGGGAGGAGG AGTGAGAGGT GGAACACAGC CTGGATATGT ATACAAGAAC
    AGCATTAAGG TGAGCTGCCT GGGACTGGAG GGGAACCTCC AAGGTGACCC TTGCCGCTTT
    GCACTGACCT CCAGAGGGCC AGAGGGTGGG ATCCAGCGCT ATGTCCTGCA GGCTGCAGAC
    CCTGCTATCA GTCAGGCCTG GATCAAGCAT GTGGCTCAGA TCTTGGAGAG CCAACGGGAC
    TTCCTCAACG CATTGCAGTC ACCCATTGAG TACCAGAGAC GGGAGAGCCA GACCAACAGC
    CTGGGGCGGC CAAGAGGGCC TGGAGTGGGG AGCCCTGGAA GAATTCGGCT TGGAGATCAG
    GCCCAGGGCA GCACACACAC ACCCATCAAT GGCTCTCTCC CCTCTCTGCT GCTGTCACCC
    AAAGGGGAGG TGGCCAGAGC CCTCTTGCCA CTGGATAAAC AGGCCCTTGG TGACATCCCC
    CAGGCTCCCC ATGACTCTCC TCCAGTCTCT CCAACTCCAA AAACCCCTCC CTGCCAAGCC
    AGACTTGCCA AGCTGGATGA AGATGAGCTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334862.1
    CDS461..2113
    Translation

    Target ORF information:

    RefSeq Version NM_001347933.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 25 (ARHGEF25), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347933.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCGGGGGG GGCACAAAGG GGGTCGCTGT GCCTGTCCCC GTGTGATCCG AAAAGTGCTG 
    GCAAAATGCG GCTGCTGCTT CGCCCGGGGG GGACGTGAAT CCTATTCCAT TGCGGGCAGT
    GAGGGGAGTA TATCGGCTTC TGCTGCCTCC GGTCTGGCTG CCCCCTCTGG CCCCAGCTCT
    GGCCTCAGCT CTGGCCCCTG TTCCCCAGGC CCCCCAGGGC CCGTCAGTGG CCTGAGGAGA
    TGGTTGGATC ATTCCAAACA TTGTCTCAGT GTGGAAACTG AGGCAGACAG TGGTCAGGCA
    GGACCATATG AGAACTGGAT GTTGGAGCCA GCTCTAGCCA CAGGAGAGGA GCTGCCGGAA
    CTGACCTTGC TGACCACACT GTTGGAGGGC CCTGGAGATA AGACGCAGCC ACCTGAAGAG
    GAGACTTTGT CCCAAGCCCC TGAGAGTGAG GAGGAACAGA AGAAGAAGGC TCTGGAAAGG
    AGTATGTATG TCCTGAGTGA ACTGGTAGAA ACAGAGAAAA TGTACGTGGA CGACTTGGGG
    CAGATTGTGG AGGGTTATAT GGCCACCATG GCTGCTCAGG GGGTCCCCGA GAGTCTTCGA
    GGCCGTGACA GGATTGTGTT TGGGAATATC CAGCAAATCT ATGAGTGGCA CCGAGACTAT
    TTCTTGCAAG AGCTACAACG GTGTCTGAAA GATCCTGATT GGCTGGCTCA GCTATTCATC
    AAACACGAGC TCCGGCAGCA GCTGGGGCAC CGCCTGCAGC TGAACGACCT CCTCATCAAA
    CCTGTGCAGC GGATCATGAA ATACCAGCTG CTGCTCAAGG ATTTTCTCAA GTATTACAAT
    AGAGCTGGGA TGGATACTGC AGACCTAGAG CAAGCTGTGG AGGTCATGTG CTTTGTGCCC
    AAGCGCTGCA ACGATATGAT GACGCTGGGG AGATTGCGGG GATTTGAGGG CAAACTGACT
    GCTCAGGGGA AGCTCTTGGG CCAGGACACT TTCTGGGTCA CCGAGCCTGA GGCTGGAGGG
    CTGCTGTCTT CCCGAGGTCG AGAGAGGCGC GTCTTCCTCT TTGAGCAAAT CATCATCTTC
    AGTGAAGCCC TGGGAGGAGG AGTGAGAGGT GGAACACAGC CTGGATATGT ATACAAGAAC
    AGCATTAAGG TGAGCTGCCT GGGACTGGAG GGGAACCTCC AAGGTGACCC TTGCCGCTTT
    GCACTGACCT CCAGAGGGCC AGAGGGTGGG ATCCAGCGCT ATGTCCTGCA GGCTGCAGAC
    CCTGCTATCA GTCAGGCCTG GATCAAGCAT GTGGCTCAGA TCTTGGAGAG CCAACGGGAC
    TTCCTCAACG CATTGCAGTC ACCCATTGAG TACCAGAGAC GGGAGAGCCA GACCAACAGC
    CTGGGGCGGC CAAGAGGGCC TGGAGTGGGG AGCCCTGGAA GAATTCGGCT TGGAGATCAG
    GCCCAGGGCA GCACACACAC ACCCATCAAT GGCTCTCTCC CCTCTCTGCT GCTGTCACCC
    AAAGGGGAGG TGGCCAGAGC CCTCTTGCCA CTGGATAAAC AGGCCCTTGG TGACATCCCC
    CAGGCTCCCC ATGACTCTCC TCCAGTCTCT CCAACTCCAA AAACCCCTCC CTGCCAAGCC
    AGACTTGCCA AGCTGGATGA AGATGAGCTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107246
    Clone ID Related Accession (Same CDS sequence) NM_182947.4
    Accession Version NM_182947.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-01
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 25 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025165.27. On Nov 23, 2018 this sequence version replaced NM_182947.3. Transcript Variant: This variant (1) represents the longest transcript (1) and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047559.1, SRR1660803.259832.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000286494.9/ ENSP00000286494.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCGGGGGG GGCACAAAGG GGGTCGCTGT GCCTGTCCCC GTGTGATCCG AAAAGTGCTG 
    GCAAAATGCG GCTGCTGCTT CGCCCGGGGG GGACGTGAAT CCTATTCCAT TGCGGGCAGT
    GAGGGGAGTA TATCGGCTTC TGCTGCCTCC GGTCTGGCTG CCCCCTCTGG CCCCAGCTCT
    GGCCTCAGCT CTGGCCCCTG TTCCCCAGGC CCCCCAGGGC CCGTCAGTGG CCTGAGGAGA
    TGGTTGGATC ATTCCAAACA TTGTCTCAGT GTGGAAACTG AGGCAGACAG TGGTCAGGCA
    GGACCATATG AGAACTGGAT GTTGGAGCCA GCTCTAGCCA CAGGAGAGGA GCTGCCGGAA
    CTGACCTTGC TGACCACACT GTTGGAGGGC CCTGGAGATA AGACGCAGCC ACCTGAAGAG
    GAGACTTTGT CCCAAGCCCC TGAGAGTGAG GAGGAACAGA AGAAGAAGGC TCTGGAAAGG
    AGTATGTATG TCCTGAGTGA ACTGGTAGAA ACAGAGAAAA TGTACGTGGA CGACTTGGGG
    CAGATTGTGG AGGGTTATAT GGCCACCATG GCTGCTCAGG GGGTCCCCGA GAGTCTTCGA
    GGCCGTGACA GGATTGTGTT TGGGAATATC CAGCAAATCT ATGAGTGGCA CCGAGACTAT
    TTCTTGCAAG AGCTACAACG GTGTCTGAAA GATCCTGATT GGCTGGCTCA GCTATTCATC
    AAACACGAGC GCCGGCTGCA TATGTATGTG GTGTACTGTC AGAATAAGCC CAAGTCAGAG
    CATGTGGTGT CAGAGTTTGG GGACAGCTAC TTTGAGGAGC TCCGGCAGCA GCTGGGGCAC
    CGCCTGCAGC TGAACGACCT CCTCATCAAA CCTGTGCAGC GGATCATGAA ATACCAGCTG
    CTGCTCAAGG ATTTTCTCAA GTATTACAAT AGAGCTGGGA TGGATACTGC AGACCTAGAG
    CAAGCTGTGG AGGTCATGTG CTTTGTGCCC AAGCGCTGCA ACGATATGAT GACGCTGGGG
    AGATTGCGGG GATTTGAGGG CAAACTGACT GCTCAGGGGA AGCTCTTGGG CCAGGACACT
    TTCTGGGTCA CCGAGCCTGA GGCTGGAGGG CTGCTGTCTT CCCGAGGTCG AGAGAGGCGC
    GTCTTCCTCT TTGAGCAAAT CATCATCTTC AGTGAAGCCC TGGGAGGAGG AGTGAGAGGT
    GGAACACAGC CTGGATATGT ATACAAGAAC AGCATTAAGG TGAGCTGCCT GGGACTGGAG
    GGGAACCTCC AAGGTGACCC TTGCCGCTTT GCACTGACCT CCAGAGGGCC AGAGGGTGGG
    ATCCAGCGCT ATGTCCTGCA GGCTGCAGAC CCTGCTATCA GTCAGGCCTG GATCAAGCAT
    GTGGCTCAGA TCTTGGAGAG CCAACGGGAC TTCCTCAACG CATTGCAGTC ACCCATTGAG
    TACCAGAGAC GGGAGAGCCA GACCAACAGC CTGGGGCGGC CAAGAGGGCC TGGAGTGGGG
    AGCCCTGGAA GAATTCAGCT TGGAGATCAG GCCCAGGGCA GCACACACAC ACCCATCAAT
    GGCTCTCTCC CCTCTCTGCT GCTGTCACCC AAAGGGGAGG TGGCCAGAGC CCTCTTGCCA
    CTGGATAAAC AGGCCCTTGG TGACATCCCC CAGGCTCCCC ATGACTCTCC TCCAGTCTCT
    CCAACTCCAA AAACCCCTCC CTGCCAAGCC AGACTTGCCA AGCTGGATGA AGATGAGCTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_891992.3
    CDS461..2203
    Translation

    Target ORF information:

    RefSeq Version NM_182947.4
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 25 (ARHGEF25), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_182947.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCGGGGGG GGCACAAAGG GGGTCGCTGT GCCTGTCCCC GTGTGATCCG AAAAGTGCTG 
    GCAAAATGCG GCTGCTGCTT CGCCCGGGGG GGACGTGAAT CCTATTCCAT TGCGGGCAGT
    GAGGGGAGTA TATCGGCTTC TGCTGCCTCC GGTCTGGCTG CCCCCTCTGG CCCCAGCTCT
    GGCCTCAGCT CTGGCCCCTG TTCCCCAGGC CCCCCAGGGC CCGTCAGTGG CCTGAGGAGA
    TGGTTGGATC ATTCCAAACA TTGTCTCAGT GTGGAAACTG AGGCAGACAG TGGTCAGGCA
    GGACCATATG AGAACTGGAT GTTGGAGCCA GCTCTAGCCA CAGGAGAGGA GCTGCCGGAA
    CTGACCTTGC TGACCACACT GTTGGAGGGC CCTGGAGATA AGACGCAGCC ACCTGAAGAG
    GAGACTTTGT CCCAAGCCCC TGAGAGTGAG GAGGAACAGA AGAAGAAGGC TCTGGAAAGG
    AGTATGTATG TCCTGAGTGA ACTGGTAGAA ACAGAGAAAA TGTACGTGGA CGACTTGGGG
    CAGATTGTGG AGGGTTATAT GGCCACCATG GCTGCTCAGG GGGTCCCCGA GAGTCTTCGA
    GGCCGTGACA GGATTGTGTT TGGGAATATC CAGCAAATCT ATGAGTGGCA CCGAGACTAT
    TTCTTGCAAG AGCTACAACG GTGTCTGAAA GATCCTGATT GGCTGGCTCA GCTATTCATC
    AAACACGAGC GCCGGCTGCA TATGTATGTG GTGTACTGTC AGAATAAGCC CAAGTCAGAG
    CATGTGGTGT CAGAGTTTGG GGACAGCTAC TTTGAGGAGC TCCGGCAGCA GCTGGGGCAC
    CGCCTGCAGC TGAACGACCT CCTCATCAAA CCTGTGCAGC GGATCATGAA ATACCAGCTG
    CTGCTCAAGG ATTTTCTCAA GTATTACAAT AGAGCTGGGA TGGATACTGC AGACCTAGAG
    CAAGCTGTGG AGGTCATGTG CTTTGTGCCC AAGCGCTGCA ACGATATGAT GACGCTGGGG
    AGATTGCGGG GATTTGAGGG CAAACTGACT GCTCAGGGGA AGCTCTTGGG CCAGGACACT
    TTCTGGGTCA CCGAGCCTGA GGCTGGAGGG CTGCTGTCTT CCCGAGGTCG AGAGAGGCGC
    GTCTTCCTCT TTGAGCAAAT CATCATCTTC AGTGAAGCCC TGGGAGGAGG AGTGAGAGGT
    GGAACACAGC CTGGATATGT ATACAAGAAC AGCATTAAGG TGAGCTGCCT GGGACTGGAG
    GGGAACCTCC AAGGTGACCC TTGCCGCTTT GCACTGACCT CCAGAGGGCC AGAGGGTGGG
    ATCCAGCGCT ATGTCCTGCA GGCTGCAGAC CCTGCTATCA GTCAGGCCTG GATCAAGCAT
    GTGGCTCAGA TCTTGGAGAG CCAACGGGAC TTCCTCAACG CATTGCAGTC ACCCATTGAG
    TACCAGAGAC GGGAGAGCCA GACCAACAGC CTGGGGCGGC CAAGAGGGCC TGGAGTGGGG
    AGCCCTGGAA GAATTCAGCT TGGAGATCAG GCCCAGGGCA GCACACACAC ACCCATCAAT
    GGCTCTCTCC CCTCTCTGCT GCTGTCACCC AAAGGGGAGG TGGCCAGAGC CCTCTTGCCA
    CTGGATAAAC AGGCCCTTGG TGACATCCCC CAGGCTCCCC ATGACTCTCC TCCAGTCTCT
    CCAACTCCAA AAACCCCTCC CTGCCAAGCC AGACTTGCCA AGCTGGATGA AGATGAGCTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108207
    Clone ID Related Accession (Same CDS sequence) NM_001111270.3
    Accession Version NM_001111270.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 25 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025165.27. On May 31, 2019 this sequence version replaced NM_001111270.2. Transcript Variant: This variant (3) differs in the 5' UTR, and in the presence and absence of exons in the 5' coding region, compared to variant 1. The resulting isoform (3) contains a distinct N-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB370196.1, SRR1660803.224487.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAGCCCC CGGACCGCCC CGCCCCTGGC CGCACTGACC GGATACTGGG GGTCATGGGG 
    GGCATGCTGC GCGCATGCGC CCTCCCTGGG CAGGAGGGGC CCCCAAGGAG AAGCCCTCTA
    GGGTTGGTGG GTACCGAGCC AGAGTCTGAA CGTACGGAGG GAGATCACAG AAGGGATCGC
    GAACATGAGG TCCTCGCCGG GGCTCTGCAG CCCGAATCCT ATTCCATTGC GGGCAGTGAG
    GGGAGTATAT CGGCTTCTGC TGCCTCCGGT CTGGCTGCCC CCTCTGGCCC CAGCTCTGGC
    CTCAGCTCTG GCCCCTGTTC CCCAGGCCCC CCAGGGCCCG TCAGTGGCCT GAGGAGATGG
    TTGGATCATT CCAAACATTG TCTCAGTGTG GAAACTGAGG CAGACAGTGG TCAGGCAGGA
    CCATATGAGA ACTGGATGTT GGAGCCAGCT CTAGCCACAG GAGAGGAGCT GCCGGAACTG
    ACCTTGCTGA CCACACTGTT GGAGGGCCCT GGAGATAAGA CGCAGCCACC TGAAGAGGAG
    ACTTTGTCCC AAGCCCCTGA GAGTGAGGAG GAACAGAAGA AGAAGGCTCT GGAAAGGAGT
    ATGTATGTCC TGAGTGAACT GGTAGAAACA GAGAAAATGT ACGTGGACGA CTTGGGGCAG
    ATTGTGGAGG GTTATATGGC CACCATGGCT GCTCAGGGGG TCCCCGAGAG TCTTCGAGGC
    CGTGACAGGA TTGTGTTTGG GAATATCCAG CAAATCTATG AGTGGCACCG AGACTATTTC
    TTGCAAGAGC TACAACGGTG TCTGAAAGAT CCTGATTGGC TGGCTCAGCT ATTCATCAAA
    CACGAGCGCC GGCTGCATAT GTATGTGGTG TACTGTCAGA ATAAGCCCAA GTCAGAGCAT
    GTGGTGTCAG AGTTTGGGGA CAGCTACTTT GAGGAGCTCC GGCAGCAGCT GGGGCACCGC
    CTGCAGCTGA ACGACCTCCT CATCAAACCT GTGCAGCGGA TCATGAAATA CCAGCTGCTG
    CTCAAGGATT TTCTCAAGTA TTACAATAGA GCTGGGATGG ATACTGCAGA CCTAGAGCAA
    GCTGTGGAGG TCATGTGCTT TGTGCCCAAG CGCTGCAACG ATATGATGAC GCTGGGGAGA
    TTGCGGGGAT TTGAGGGCAA ACTGACTGCT CAGGGGAAGC TCTTGGGCCA GGACACTTTC
    TGGGTCACCG AGCCTGAGGC TGGAGGGCTG CTGTCTTCCC GAGGTCGAGA GAGGCGCGTC
    TTCCTCTTTG AGCAAATCAT CATCTTCAGT GAAGCCCTGG GAGGAGGAGT GAGAGGTGGA
    ACACAGCCTG GATATGTATA CAAGAACAGC ATTAAGGTGA GCTGCCTGGG ACTGGAGGGG
    AACCTCCAAG GTGACCCTTG CCGCTTTGCA CTGACCTCCA GAGGGCCAGA GGGTGGGATC
    CAGCGCTATG TCCTGCAGGC TGCAGACCCT GCTATCAGTC AGGCCTGGAT CAAGCATGTG
    GCTCAGATCT TGGAGAGCCA ACGGGACTTC CTCAACGCAT TGCAGTCACC CATTGAGTAC
    CAGAGACGGG AGAGCCAGAC CAACAGCCTG GGGCGGCCAA GAGGGCCTGG AGTGGGGAGC
    CCTGGAAGAA TTCAGCTTGG AGATCAGGCC CAGGGCAGCA CACACACACC CATCAATGGC
    TCTCTCCCCT CTCTGCTGCT GTCACCCAAA GGGGAGGTGG CCAGAGCCCT CTTGCCACTG
    GATAAACAGG CCCTTGGTGA CATCCCCCAG GCTCCCCATG ACTCTCCTCC AGTCTCTCCA
    ACTCCAAAAA CCCCTCCCTG CCAAGCCAGA CTTGCCAAGC TGGATGAAGA TGAGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001104740.2
    CDS102..1961
    Translation

    Target ORF information:

    RefSeq Version NM_001111270.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 25 (ARHGEF25), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001111270.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAAGCCCC CGGACCGCCC CGCCCCTGGC CGCACTGACC GGATACTGGG GGTCATGGGG 
    GGCATGCTGC GCGCATGCGC CCTCCCTGGG CAGGAGGGGC CCCCAAGGAG AAGCCCTCTA
    GGGTTGGTGG GTACCGAGCC AGAGTCTGAA CGTACGGAGG GAGATCACAG AAGGGATCGC
    GAACATGAGG TCCTCGCCGG GGCTCTGCAG CCCGAATCCT ATTCCATTGC GGGCAGTGAG
    GGGAGTATAT CGGCTTCTGC TGCCTCCGGT CTGGCTGCCC CCTCTGGCCC CAGCTCTGGC
    CTCAGCTCTG GCCCCTGTTC CCCAGGCCCC CCAGGGCCCG TCAGTGGCCT GAGGAGATGG
    TTGGATCATT CCAAACATTG TCTCAGTGTG GAAACTGAGG CAGACAGTGG TCAGGCAGGA
    CCATATGAGA ACTGGATGTT GGAGCCAGCT CTAGCCACAG GAGAGGAGCT GCCGGAACTG
    ACCTTGCTGA CCACACTGTT GGAGGGCCCT GGAGATAAGA CGCAGCCACC TGAAGAGGAG
    ACTTTGTCCC AAGCCCCTGA GAGTGAGGAG GAACAGAAGA AGAAGGCTCT GGAAAGGAGT
    ATGTATGTCC TGAGTGAACT GGTAGAAACA GAGAAAATGT ACGTGGACGA CTTGGGGCAG
    ATTGTGGAGG GTTATATGGC CACCATGGCT GCTCAGGGGG TCCCCGAGAG TCTTCGAGGC
    CGTGACAGGA TTGTGTTTGG GAATATCCAG CAAATCTATG AGTGGCACCG AGACTATTTC
    TTGCAAGAGC TACAACGGTG TCTGAAAGAT CCTGATTGGC TGGCTCAGCT ATTCATCAAA
    CACGAGCGCC GGCTGCATAT GTATGTGGTG TACTGTCAGA ATAAGCCCAA GTCAGAGCAT
    GTGGTGTCAG AGTTTGGGGA CAGCTACTTT GAGGAGCTCC GGCAGCAGCT GGGGCACCGC
    CTGCAGCTGA ACGACCTCCT CATCAAACCT GTGCAGCGGA TCATGAAATA CCAGCTGCTG
    CTCAAGGATT TTCTCAAGTA TTACAATAGA GCTGGGATGG ATACTGCAGA CCTAGAGCAA
    GCTGTGGAGG TCATGTGCTT TGTGCCCAAG CGCTGCAACG ATATGATGAC GCTGGGGAGA
    TTGCGGGGAT TTGAGGGCAA ACTGACTGCT CAGGGGAAGC TCTTGGGCCA GGACACTTTC
    TGGGTCACCG AGCCTGAGGC TGGAGGGCTG CTGTCTTCCC GAGGTCGAGA GAGGCGCGTC
    TTCCTCTTTG AGCAAATCAT CATCTTCAGT GAAGCCCTGG GAGGAGGAGT GAGAGGTGGA
    ACACAGCCTG GATATGTATA CAAGAACAGC ATTAAGGTGA GCTGCCTGGG ACTGGAGGGG
    AACCTCCAAG GTGACCCTTG CCGCTTTGCA CTGACCTCCA GAGGGCCAGA GGGTGGGATC
    CAGCGCTATG TCCTGCAGGC TGCAGACCCT GCTATCAGTC AGGCCTGGAT CAAGCATGTG
    GCTCAGATCT TGGAGAGCCA ACGGGACTTC CTCAACGCAT TGCAGTCACC CATTGAGTAC
    CAGAGACGGG AGAGCCAGAC CAACAGCCTG GGGCGGCCAA GAGGGCCTGG AGTGGGGAGC
    CCTGGAAGAA TTCAGCTTGG AGATCAGGCC CAGGGCAGCA CACACACACC CATCAATGGC
    TCTCTCCCCT CTCTGCTGCT GTCACCCAAA GGGGAGGTGG CCAGAGCCCT CTTGCCACTG
    GATAAACAGG CCCTTGGTGA CATCCCCCAG GCTCCCCATG ACTCTCCTCC AGTCTCTCCA
    ACTCCAAAAA CCCCTCCCTG CCAAGCCAGA CTTGCCAAGC TGGATGAAGA TGAGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108220
    Clone ID Related Accession (Same CDS sequence) NM_001347933.2
    Accession Version NM_001347933.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1653bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product rho guanine nucleotide exchange factor 25 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025165.27. On May 31, 2019 this sequence version replaced NM_001347933.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.507652.1, SRR1660803.156503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCGGGGGG GGCACAAAGG GGGTCGCTGT GCCTGTCCCC GTGTGATCCG AAAAGTGCTG 
    GCAAAATGCG GCTGCTGCTT CGCCCGGGGG GGACGTGAAT CCTATTCCAT TGCGGGCAGT
    GAGGGGAGTA TATCGGCTTC TGCTGCCTCC GGTCTGGCTG CCCCCTCTGG CCCCAGCTCT
    GGCCTCAGCT CTGGCCCCTG TTCCCCAGGC CCCCCAGGGC CCGTCAGTGG CCTGAGGAGA
    TGGTTGGATC ATTCCAAACA TTGTCTCAGT GTGGAAACTG AGGCAGACAG TGGTCAGGCA
    GGACCATATG AGAACTGGAT GTTGGAGCCA GCTCTAGCCA CAGGAGAGGA GCTGCCGGAA
    CTGACCTTGC TGACCACACT GTTGGAGGGC CCTGGAGATA AGACGCAGCC ACCTGAAGAG
    GAGACTTTGT CCCAAGCCCC TGAGAGTGAG GAGGAACAGA AGAAGAAGGC TCTGGAAAGG
    AGTATGTATG TCCTGAGTGA ACTGGTAGAA ACAGAGAAAA TGTACGTGGA CGACTTGGGG
    CAGATTGTGG AGGGTTATAT GGCCACCATG GCTGCTCAGG GGGTCCCCGA GAGTCTTCGA
    GGCCGTGACA GGATTGTGTT TGGGAATATC CAGCAAATCT ATGAGTGGCA CCGAGACTAT
    TTCTTGCAAG AGCTACAACG GTGTCTGAAA GATCCTGATT GGCTGGCTCA GCTATTCATC
    AAACACGAGC TCCGGCAGCA GCTGGGGCAC CGCCTGCAGC TGAACGACCT CCTCATCAAA
    CCTGTGCAGC GGATCATGAA ATACCAGCTG CTGCTCAAGG ATTTTCTCAA GTATTACAAT
    AGAGCTGGGA TGGATACTGC AGACCTAGAG CAAGCTGTGG AGGTCATGTG CTTTGTGCCC
    AAGCGCTGCA ACGATATGAT GACGCTGGGG AGATTGCGGG GATTTGAGGG CAAACTGACT
    GCTCAGGGGA AGCTCTTGGG CCAGGACACT TTCTGGGTCA CCGAGCCTGA GGCTGGAGGG
    CTGCTGTCTT CCCGAGGTCG AGAGAGGCGC GTCTTCCTCT TTGAGCAAAT CATCATCTTC
    AGTGAAGCCC TGGGAGGAGG AGTGAGAGGT GGAACACAGC CTGGATATGT ATACAAGAAC
    AGCATTAAGG TGAGCTGCCT GGGACTGGAG GGGAACCTCC AAGGTGACCC TTGCCGCTTT
    GCACTGACCT CCAGAGGGCC AGAGGGTGGG ATCCAGCGCT ATGTCCTGCA GGCTGCAGAC
    CCTGCTATCA GTCAGGCCTG GATCAAGCAT GTGGCTCAGA TCTTGGAGAG CCAACGGGAC
    TTCCTCAACG CATTGCAGTC ACCCATTGAG TACCAGAGAC GGGAGAGCCA GACCAACAGC
    CTGGGGCGGC CAAGAGGGCC TGGAGTGGGG AGCCCTGGAA GAATTCAGCT TGGAGATCAG
    GCCCAGGGCA GCACACACAC ACCCATCAAT GGCTCTCTCC CCTCTCTGCT GCTGTCACCC
    AAAGGGGAGG TGGCCAGAGC CCTCTTGCCA CTGGATAAAC AGGCCCTTGG TGACATCCCC
    CAGGCTCCCC ATGACTCTCC TCCAGTCTCT CCAACTCCAA AAACCCCTCC CTGCCAAGCC
    AGACTTGCCA AGCTGGATGA AGATGAGCTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334862.2
    CDS461..2113
    Translation

    Target ORF information:

    RefSeq Version NM_001347933.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Rho guanine nucleotide exchange factor 25 (ARHGEF25), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347933.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGCGGGGGG GGCACAAAGG GGGTCGCTGT GCCTGTCCCC GTGTGATCCG AAAAGTGCTG 
    GCAAAATGCG GCTGCTGCTT CGCCCGGGGG GGACGTGAAT CCTATTCCAT TGCGGGCAGT
    GAGGGGAGTA TATCGGCTTC TGCTGCCTCC GGTCTGGCTG CCCCCTCTGG CCCCAGCTCT
    GGCCTCAGCT CTGGCCCCTG TTCCCCAGGC CCCCCAGGGC CCGTCAGTGG CCTGAGGAGA
    TGGTTGGATC ATTCCAAACA TTGTCTCAGT GTGGAAACTG AGGCAGACAG TGGTCAGGCA
    GGACCATATG AGAACTGGAT GTTGGAGCCA GCTCTAGCCA CAGGAGAGGA GCTGCCGGAA
    CTGACCTTGC TGACCACACT GTTGGAGGGC CCTGGAGATA AGACGCAGCC ACCTGAAGAG
    GAGACTTTGT CCCAAGCCCC TGAGAGTGAG GAGGAACAGA AGAAGAAGGC TCTGGAAAGG
    AGTATGTATG TCCTGAGTGA ACTGGTAGAA ACAGAGAAAA TGTACGTGGA CGACTTGGGG
    CAGATTGTGG AGGGTTATAT GGCCACCATG GCTGCTCAGG GGGTCCCCGA GAGTCTTCGA
    GGCCGTGACA GGATTGTGTT TGGGAATATC CAGCAAATCT ATGAGTGGCA CCGAGACTAT
    TTCTTGCAAG AGCTACAACG GTGTCTGAAA GATCCTGATT GGCTGGCTCA GCTATTCATC
    AAACACGAGC TCCGGCAGCA GCTGGGGCAC CGCCTGCAGC TGAACGACCT CCTCATCAAA
    CCTGTGCAGC GGATCATGAA ATACCAGCTG CTGCTCAAGG ATTTTCTCAA GTATTACAAT
    AGAGCTGGGA TGGATACTGC AGACCTAGAG CAAGCTGTGG AGGTCATGTG CTTTGTGCCC
    AAGCGCTGCA ACGATATGAT GACGCTGGGG AGATTGCGGG GATTTGAGGG CAAACTGACT
    GCTCAGGGGA AGCTCTTGGG CCAGGACACT TTCTGGGTCA CCGAGCCTGA GGCTGGAGGG
    CTGCTGTCTT CCCGAGGTCG AGAGAGGCGC GTCTTCCTCT TTGAGCAAAT CATCATCTTC
    AGTGAAGCCC TGGGAGGAGG AGTGAGAGGT GGAACACAGC CTGGATATGT ATACAAGAAC
    AGCATTAAGG TGAGCTGCCT GGGACTGGAG GGGAACCTCC AAGGTGACCC TTGCCGCTTT
    GCACTGACCT CCAGAGGGCC AGAGGGTGGG ATCCAGCGCT ATGTCCTGCA GGCTGCAGAC
    CCTGCTATCA GTCAGGCCTG GATCAAGCAT GTGGCTCAGA TCTTGGAGAG CCAACGGGAC
    TTCCTCAACG CATTGCAGTC ACCCATTGAG TACCAGAGAC GGGAGAGCCA GACCAACAGC
    CTGGGGCGGC CAAGAGGGCC TGGAGTGGGG AGCCCTGGAA GAATTCAGCT TGGAGATCAG
    GCCCAGGGCA GCACACACAC ACCCATCAAT GGCTCTCTCC CCTCTCTGCT GCTGTCACCC
    AAAGGGGAGG TGGCCAGAGC CCTCTTGCCA CTGGATAAAC AGGCCCTTGG TGACATCCCC
    CAGGCTCCCC ATGACTCTCC TCCAGTCTCT CCAACTCCAA AAACCCCTCC CTGCCAAGCC
    AGACTTGCCA AGCTGGATGA AGATGAGCTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.