DDIT4L cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDIT4L
Entrez Gene ID	115265
Full Name	DNA damage inducible transcript 4 like
Synonyms	REDD2,Rtp801L
General protein information	Preferred Names DNA damage inducible transcript 4 like Names DNA damage-inducible transcript 4-like protein HIF-1 responsive protein RTP801-like REDD-2 homolog of mouse SMHS1 protein regulated in development and DNA damage response 2 regulated in development and DNA damage response 2
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 4 Map Location: 4q24
Disorder MIM:	607730

mRNA and Protein(s)

mRNA	Protein	Name
NM_145244.4	NP_660287.1	DNA damage-inducible transcript 4-like protein
NM_145244.3	NP_660287.1	DNA damage-inducible transcript 4-like protein
XM_005262721.1	XP_005262778.1	DNA damage-inducible transcript 4-like protein isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DDIT4L	XP_544999.2
Bos taurus (cattle)	DDIT4L	NP_001074988.1
Pan troglodytes (chimpanzee)	DDIT4L	XP_001167944.1
Mus musculus (house mouse)	Ddit4l	NP_084419.2
Xenopus tropicalis (tropical clawed frog)	ddit4l	XP_004911505.1
Homo sapiens (human)	DDIT4L	NP_660287.1
	DDIT4L	XP_001108176.1
Rattus norvegicus (Norway rat)	LOC100363484	XP_002729167.1

Gene Ontology
Bibliography

Related articles in PubMed

DDiT4L promotes autophagy and inhibits pathological cardiac hypertrophy in response to stress.
Simonson B, Subramanya V, Chan MC, Zhang A, Franchino H, Ottaviano F, Mishra MK, Knight AC, Hunt D, Ghiran I, Khurana TS, Kontaridis MI, Rosenzweig A, Das S
Science signaling10(468)(2017 Feb)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

An inter-species protein-protein interaction network across vast evolutionary distance.
Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Ta?an M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barab?si AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M
Molecular systems biology12(4)865(2016 Apr)

Genome-wide screen of promoter methylation identifies novel markers in melanoma.
Koga Y, Pelizzola M, Cheng E, Krauthammer M, Sznol M, Ariyan S, Narayan D, Molinaro AM, Halaban R, Weissman SM
Genome research19(8)1462-70(2009 Aug)

The regulated in development and DNA damage response 2 (REDD2) gene mediates human monocyte cell death through a reduction in thioredoxin-1 expression.
Imen JS, Billiet L, Cuaz-P?rolin C, Michaud N, Rouis M
Free radical biology & medicine46(10)1404-10(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Promoter methylation and differential gene expression of five markers: COL1A2, NPM2, HSPB6, DDIT4L and MT1G were validated by sequencing of bisulfite-modified DNA and real-time reverse transcriptase PCR, respectively.
Title: Genome-wide screen of promoter methylation identifies novel markers in melanoma.

mediates monocyte cell death through a reduction in thioredoxin-1 expression
Title: The regulated in development and DNA damage response 2 (REDD2) gene mediates human monocyte cell death through a reduction in thioredoxin-1 expression.

RTP801 and RTP801L work downstream of AKT and upstream of TSC2 to inhibit mTOR functions
Title: The stress-inducted proteins RTP801 and RTP801L are negative regulators of the mammalian target of rapamycin pathway.

The following DDIT4L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDIT4L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32161	NM_145244.4 Latest version!	Homo sapiens DNA damage inducible transcript 4 like (DDIT4L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu32161	XM_005262721.1 Latest version!	Homo sapiens DNA-damage-inducible transcript 4-like (DDIT4L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu32161	NM_145244.3	Homo sapiens DNA damage inducible transcript 4 like (DDIT4L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDIT4L ( NM_145244.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_660287.1 Homo sapiens DNA damage inducible transcript 4 like (DDIT4L), mRNA.

Price & Availability↑

CloneID	OHu32161
Clone ID Related Accession (Same CDS sequence)	NM_145244.4 , NM_145244.3 , XM_005262721.1
Accession Version	NM_145244.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	DNA damage-inducible transcript 4-like protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK094236.1 and BC013592.2. On Nov 22, 2018 this sequence version replaced NM_145244.3. ##Evidence-Data-START## Transcript exon combination :: AK094236.1, SRR1803611.125486.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674, SAMN03267753 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000273990.6/ ENSP00000354830.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTTGCAA CTGGCAGTTT GAGCAGCAAG AACCCGGCCA GCATTTCAGA ATTGCTGGAC 
TGTGGCTATC ACCCAGAGAG CCTGCTAAGT GATTTTGACT ACTGGGATTA TGTTGTTCCT 
GAACCCAACC TCAACGAGGT AATATTTGAG GAATCAACTT GCCAGAATTT GGTTAAAATG 
CTGGAGAACT GTCTGTCCAA ATCAAAGCAA ACTAAACTTG GTTGCTCAAA GGTCCTTGTC 
CCTGAGAAAC TGACCCAGAG AATTGCTCAA GATGTCCTGC GGCTTTCCTC AACGGAGCCC 
TGCGGCTTGC GAGGTTGTGT TATGCACGTG AACTTGGAAA TTGAAAATGT ATGTAAAAAG 
CTGGATAGGA TTGTGTGTGA TTCTAGCGTC GTACCTACTT TTGAGCTTAC ACTTGTGTTT 
AAGCAGGAGA ACTGCTCATG GACTAGCTTC AGGGACTTTT TCTTTAGTAG AGGTCGCTTC 
TCCTCTGGTT TCAGGAGAAC TCTGATCCTC AGCTCAGGAT TTCGACTTGT TAAGAAAAAA 
CTTTACTCAC TGATTGGAAC AACAGTGATT GAAGGGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660287.1
CDS	216..797
Translation	MVATGSLSSKNPASISELLDCGYHPESLLSDFDYWDYVVPEPNLNEVIFEESTCQNLVKMLENCLSKSKQTKLGCSKVLVPEKLTQRIAQDVLRLSSTEPCGLRGCVMHVNLEIENVCKKLDRIVCDSSVVPTFELTLVFKQENCSWTSFRDFFFSRGRFSSGFRRTLILSSGFRLVKKKLYSLIGTTVIEGS

Target ORF information:

RefSeq Version	NM_145244.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA damage inducible transcript 4 like (DDIT4L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145244.4

ORF Insert Sequence:

ATGGTTGCAA CTGGCAGTTT GAGCAGCAAG AACCCGGCCA GCATTTCAGA ATTGCTGGAC 
TGTGGCTATC ACCCAGAGAG CCTGCTAAGT GATTTTGACT ACTGGGATTA TGTTGTTCCT 
GAACCCAACC TCAACGAGGT AATATTTGAG GAATCAACTT GCCAGAATTT GGTTAAAATG 
CTGGAGAACT GTCTGTCCAA ATCAAAGCAA ACTAAACTTG GTTGCTCAAA GGTCCTTGTC 
CCTGAGAAAC TGACCCAGAG AATTGCTCAA GATGTCCTGC GGCTTTCCTC AACGGAGCCC 
TGCGGCTTGC GAGGTTGTGT TATGCACGTG AACTTGGAAA TTGAAAATGT ATGTAAAAAG 
CTGGATAGGA TTGTGTGTGA TTCTAGCGTC GTACCTACTT TTGAGCTTAC ACTTGTGTTT 
AAGCAGGAGA ACTGCTCATG GACTAGCTTC AGGGACTTTT TCTTTAGTAG AGGTCGCTTC 
TCCTCTGGTT TCAGGAGAAC TCTGATCCTC AGCTCAGGAT TTCGACTTGT TAAGAAAAAA 
CTTTACTCAC TGATTGGAAC AACAGTGATT GAAGGGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDIT4L ( NM_145244.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_660287.1 Homo sapiens DNA damage inducible transcript 4 like (DDIT4L), mRNA.

Price & Availability↑

CloneID	OHu32161
Clone ID Related Accession (Same CDS sequence)	NM_145244.4 , NM_145244.3 , XM_005262721.1
Accession Version	NM_145244.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	DNA damage-inducible transcript 4-like protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB208869.1, AK094236.1 and BC013592.2. On Sep 25, 2010 this sequence version replaced NM_145244.2. ##Evidence-Data-START## Transcript exon combination :: BC013592.2, SRR1803611.72250.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674, SAMN03267753 [ECO:0000348] ##Evidence-Data-END##

ATGGTTGCAA CTGGCAGTTT GAGCAGCAAG AACCCGGCCA GCATTTCAGA ATTGCTGGAC 
TGTGGCTATC ACCCAGAGAG CCTGCTAAGT GATTTTGACT ACTGGGATTA TGTTGTTCCT 
GAACCCAACC TCAACGAGGT AATATTTGAG GAATCAACTT GCCAGAATTT GGTTAAAATG 
CTGGAGAACT GTCTGTCCAA ATCAAAGCAA ACTAAACTTG GTTGCTCAAA GGTCCTTGTC 
CCTGAGAAAC TGACCCAGAG AATTGCTCAA GATGTCCTGC GGCTTTCCTC AACGGAGCCC 
TGCGGCTTGC GAGGTTGTGT TATGCACGTG AACTTGGAAA TTGAAAATGT ATGTAAAAAG 
CTGGATAGGA TTGTGTGTGA TTCTAGCGTC GTACCTACTT TTGAGCTTAC ACTTGTGTTT 
AAGCAGGAGA ACTGCTCATG GACTAGCTTC AGGGACTTTT TCTTTAGTAG AGGTCGCTTC 
TCCTCTGGTT TCAGGAGAAC TCTGATCCTC AGCTCAGGAT TTCGACTTGT TAAGAAAAAA 
CTTTACTCAC TGATTGGAAC AACAGTGATT GAAGGGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660287.1
CDS	246..827
Translation	MVATGSLSSKNPASISELLDCGYHPESLLSDFDYWDYVVPEPNLNEVIFEESTCQNLVKMLENCLSKSKQTKLGCSKVLVPEKLTQRIAQDVLRLSSTEPCGLRGCVMHVNLEIENVCKKLDRIVCDSSVVPTFELTLVFKQENCSWTSFRDFFFSRGRFSSGFRRTLILSSGFRLVKKKLYSLIGTTVIEGS

Target ORF information:

RefSeq Version	NM_145244.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA damage inducible transcript 4 like (DDIT4L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145244.3

ORF Insert Sequence:

ATGGTTGCAA CTGGCAGTTT GAGCAGCAAG AACCCGGCCA GCATTTCAGA ATTGCTGGAC 
TGTGGCTATC ACCCAGAGAG CCTGCTAAGT GATTTTGACT ACTGGGATTA TGTTGTTCCT 
GAACCCAACC TCAACGAGGT AATATTTGAG GAATCAACTT GCCAGAATTT GGTTAAAATG 
CTGGAGAACT GTCTGTCCAA ATCAAAGCAA ACTAAACTTG GTTGCTCAAA GGTCCTTGTC 
CCTGAGAAAC TGACCCAGAG AATTGCTCAA GATGTCCTGC GGCTTTCCTC AACGGAGCCC 
TGCGGCTTGC GAGGTTGTGT TATGCACGTG AACTTGGAAA TTGAAAATGT ATGTAAAAAG 
CTGGATAGGA TTGTGTGTGA TTCTAGCGTC GTACCTACTT TTGAGCTTAC ACTTGTGTTT 
AAGCAGGAGA ACTGCTCATG GACTAGCTTC AGGGACTTTT TCTTTAGTAG AGGTCGCTTC 
TCCTCTGGTT TCAGGAGAAC TCTGATCCTC AGCTCAGGAT TTCGACTTGT TAAGAAAAAA 
CTTTACTCAC TGATTGGAAC AACAGTGATT GAAGGGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDIT4L ( XM_005262721.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_005262778.1 Homo sapiens DNA-damage-inducible transcript 4-like (DDIT4L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu32161
Clone ID Related Accession (Same CDS sequence)	NM_145244.4 , NM_145244.3 , XM_005262721.1
Accession Version	XM_005262721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	DNA damage-inducible transcript 4-like protein isoform X1
Comment

ATGGTTGCAA CTGGCAGTTT GAGCAGCAAG AACCCGGCCA GCATTTCAGA ATTGCTGGAC 
TGTGGCTATC ACCCAGAGAG CCTGCTAAGT GATTTTGACT ACTGGGATTA TGTTGTTCCT 
GAACCCAACC TCAACGAGGT AATATTTGAG GAATCAACTT GCCAGAATTT GGTTAAAATG 
CTGGAGAACT GTCTGTCCAA ATCAAAGCAA ACTAAACTTG GTTGCTCAAA GGTCCTTGTC 
CCTGAGAAAC TGACCCAGAG AATTGCTCAA GATGTCCTGC GGCTTTCCTC AACGGAGCCC 
TGCGGCTTGC GAGGTTGTGT TATGCACGTG AACTTGGAAA TTGAAAATGT ATGTAAAAAG 
CTGGATAGGA TTGTGTGTGA TTCTAGCGTC GTACCTACTT TTGAGCTTAC ACTTGTGTTT 
AAGCAGGAGA ACTGCTCATG GACTAGCTTC AGGGACTTTT TCTTTAGTAG AGGTCGCTTC 
TCCTCTGGTT TCAGGAGAAC TCTGATCCTC AGCTCAGGAT TTCGACTTGT TAAGAAAAAA 
CTTTACTCAC TGATTGGAAC AACAGTGATT GAAGGGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005262778.1
Translation	MVATGSLSSKNPASISELLDCGYHPESLLSDFDYWDYVVPEPNLNEVIFEESTCQNLVKMLENCLSKSKQTKLGCSKVLVPEKLTQRIAQDVLRLSSTEPCGLRGCVMHVNLEIENVCKKLDRIVCDSSVVPTFELTLVFKQENCSWTSFRDFFFSRGRFSSGFRRTLILSSGFRLVKKKLYSLIGTTVIEGS

Target ORF information:

RefSeq Version	XM_005262721.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens DNA-damage-inducible transcript 4-like (DDIT4L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262721.1

ORF Insert Sequence:

ATGGTTGCAA CTGGCAGTTT GAGCAGCAAG AACCCGGCCA GCATTTCAGA ATTGCTGGAC 
TGTGGCTATC ACCCAGAGAG CCTGCTAAGT GATTTTGACT ACTGGGATTA TGTTGTTCCT 
GAACCCAACC TCAACGAGGT AATATTTGAG GAATCAACTT GCCAGAATTT GGTTAAAATG 
CTGGAGAACT GTCTGTCCAA ATCAAAGCAA ACTAAACTTG GTTGCTCAAA GGTCCTTGTC 
CCTGAGAAAC TGACCCAGAG AATTGCTCAA GATGTCCTGC GGCTTTCCTC AACGGAGCCC 
TGCGGCTTGC GAGGTTGTGT TATGCACGTG AACTTGGAAA TTGAAAATGT ATGTAAAAAG 
CTGGATAGGA TTGTGTGTGA TTCTAGCGTC GTACCTACTT TTGAGCTTAC ACTTGTGTTT 
AAGCAGGAGA ACTGCTCATG GACTAGCTTC AGGGACTTTT TCTTTAGTAG AGGTCGCTTC 
TCCTCTGGTT TCAGGAGAAC TCTGATCCTC AGCTCAGGAT TTCGACTTGT TAAGAAAAAA 
CTTTACTCAC TGATTGGAAC AACAGTGATT GAAGGGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DDiT4L promotes autophagy and inhibits pathological cardiac hypertrophy in response to stress.
Science signaling10(468)(2017 Feb)
Simonson B,Subramanya V,Chan MC,Zhang A,Franchino H,Ottaviano F,Mishra MK,Knight AC,Hunt D,Ghiran I,Khurana TS,Kontaridis MI,Rosenzweig A,Das S

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

An inter-species protein-protein interaction network across vast evolutionary distance.
Molecular systems biology12(4)865(2016 Apr)
Zhong Q,Pevzner SJ,Hao T,Wang Y,Mosca R,Menche J,Taipale M,Ta?an M,Fan C,Yang X,Haley P,Murray RR,Mer F,Gebreab F,Tam S,MacWilliams A,Dricot A,Reichert P,Santhanam B,Ghamsari L,Calderwood MA,Rolland T,Charloteaux B,Lindquist S,Barab?si AL,Hill DE,Aloy P,Cusick ME,Xia Y,Roth FP,Vidal M

Genome-wide screen of promoter methylation identifies novel markers in melanoma.
Genome research19(8)1462-70(2009 Aug)
Koga Y,Pelizzola M,Cheng E,Krauthammer M,Sznol M,Ariyan S,Narayan D,Molinaro AM,Halaban R,Weissman SM

The regulated in development and DNA damage response 2 (REDD2) gene mediates human monocyte cell death through a reduction in thioredoxin-1 expression.
Free radical biology & medicine46(10)1404-10(2009 May)
Imen JS,Billiet L,Cuaz-P?rolin C,Michaud N,Rouis M

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DDIT4L cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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