CHRNE cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRNE
Entrez Gene ID	1145
Full Name	cholinergic receptor nicotinic epsilon subunit
Synonyms	ACHRE,CMS1D,CMS1E,CMS2A,CMS4A,CMS4B,CMS4C,FCCMS,SCCMS
General protein information	Preferred Names cholinergic receptor nicotinic epsilon subunit Names acetylcholine receptor subunit epsilon AchR epsilon subunit acetylcholine receptor, nicotinic, epsilon (muscle) cholinergic receptor, nicotinic epsilon cholinergic receptor, nicotinic, epsilon (muscle) cholinergic receptor, nicotinic, epsilon polypeptide
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.2
Summary	Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome. [provided by RefSeq, Sep 2009].
Disorder MIM:	100725
Disorder Html:	Myasthenic syndrome, congenital, 4A, slow-channel, 605809 (3); Myasthenic syndrome, congenital, 4B, fast-channel, 616324 (3); Myasthenic syndrome, congenital, 4C, associated with acetylcholine receptor deficiency, 608931 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000080.4	NP_000071.1	acetylcholine receptor subunit epsilon precursor
NM_000080.3	NP_000071.1	acetylcholine receptor subunit epsilon precursor
XM_017024115.1	XP_016879604.1	acetylcholine receptor subunit epsilon isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHRNE	NP_000071.1
Rattus norvegicus (Norway rat)	Chrne	NP_058890.1
Xenopus tropicalis (tropical clawed frog)	chrne	XP_002941867.2
Pan troglodytes (chimpanzee)	CHRNE	XP_523556.4
Canis lupus familiaris (dog)	CHRNE	XP_536608.3
Bos taurus (cattle)	CHRNE	NP_776697.1
	CHRNE	XP_001097094.1
Mus musculus (house mouse)	Chrne	NP_033733.1

Gene Ontology
Bibliography

Related articles in PubMed

Congenital myasthenic syndrome in Israel: Genetic and clinical characterization.
Aharoni S, Sadeh M, Shapira Y, Edvardson S, Daana M, Dor-Wollman T, Mimouni-Bloch A, Halevy A, Cohen R, Sagie L, Argov Z, Rabie M, Spiegel R, Chervinsky I, Orenstein N, Engel AG, Nevo Y
Neuromuscular disorders : NMD27(2)136-140(2017 Feb)

Mutations Causing Slow-Channel Myasthenia Reveal That a Valine Ring in the Channel Pore of Muscle AChR is Optimized for Stabilizing Channel Gating.
Shen XM, Okuno T, Milone M, Otsuka K, Takahashi K, Komaki H, Giles E, Ohno K, Engel AG
Human mutation37(10)1051-9(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study traced the cause of congenital myasthenia syndrome in unrelated patients with dominant missense mutations in the M2 domain of AChR. A valine residue is replaced by a smaller alanine either in the epsilon subunit or an equivalent position in the beta one. Also, each valine in the valine ring was found to contribute to channel kinetics equally, and the valine ring has bee optimized during evolution to govern channe...
Title: Mutations Causing Slow-Channel Myasthenia Reveal That a Valine Ring in the Channel Pore of Muscle AChR is Optimized for Stabilizing Channel Gating.

This study provied that new mouse model for the slow-channel congenital myasthenic syndrome induced by the AChR epsilonL221F mutation.
Title: A new mouse model for the slow-channel congenital myasthenic syndrome induced by the AChR ?L221F mutation.

mutational analysis of CHRNE revealed a homozygous 1293insG, which is a well-known low-expressor receptor mutation in patients with epidermolysis bullosa simplex and congenital myasthenic syndrome.
Title: Congenital myasthenic syndrome associated with epidermolysis bullosa caused by homozygous mutations in PLEC1 and CHRNE.

Targeting nAChR could offer a strategy for reducing neurodegeneration secondary to hyperphosphorylation of protein tau.
Title: Neurotoxicity induced by okadaic acid in the human neuroblastoma SH-SY5Y line can be differentially prevented by ?7 and ?2* nicotinic stimulation.

The mutations in the varepsilon subunit altered Ca(2+) permeability of AChR-channels, with varepsilon(L269F) increasing P(f) and varepsilon(I257F) decreasing it.
Title: Modulation of the Ca(2+) permeability of human endplate acetylcholine receptor-channel.

The following CHRNE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20760	NM_000080.4 Latest version!	Homo sapiens cholinergic receptor nicotinic epsilon subunit (CHRNE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu86547	XM_017024115.1 Latest version!	Homo sapiens cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu20760	NM_000080.3	Homo sapiens cholinergic receptor nicotinic epsilon subunit (CHRNE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRNE ( NM_000080.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000071.1 Homo sapiens cholinergic receptor nicotinic epsilon subunit (CHRNE), mRNA.

Price & Availability↑

CloneID	OHu20760
Clone ID Related Accession (Same CDS sequence)	NM_000080.4 , NM_000080.3
Accession Version	NM_000080.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	acetylcholine receptor subunit epsilon precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC233723.2 and X66403.1. This sequence is a reference standard in the RefSeqGene project. On Jul 24, 2018 this sequence version replaced NM_000080.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X66403.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159607 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000649488.2/ ENSP00000497829.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG 
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC 
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG 
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC 
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC 
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT 
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG 
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT 
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC 
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG 
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCGGCGCA GGCCGGCGGC CAGAAATGCA CGGTCTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTCATT GCCCAGAAAA TCCCAGAGAC TTCTCTGAGC 
GTGCCGCTCC TGGGCAGGTT CCTTATTTTC GTCATGGTGG TCGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCCCAG CGGACGCCCA CCACCCACGC CATGTCCCCG 
CGGCTGCGCC ACGTTCTCCT GGAGCTGCTG CCGCGCCTCC TGGGCTCCCC GCCGCCGCCC 
GAGGCCCCCC GGGCCGCCTC GCCCCCAAGG CGGGCGTCGT CGGTGGGCTT ATTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCACGG AGCGAGCTCG TGTTTGAGGG GCAGAGGCAC 
CGGCAGGGGA CCTGGACGGC TGCCTTCTGC CAGAGCCTGG GCGCCGCCGC CCCCGAGGTC 
CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCCGAGAGCA CGAGAGATCA GGAGGCCACC 
GGCGAGGAAG TGTCCGACTG GGTGCGCATG GGGAATGCCC TTGACAACAT CTGCTTCTGG 
GCCGCTCTGG TGCTCTTCAG CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC 
CGAGTGCCTG ATCTCCCCTA CGCGCCGTGT ATCCAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000071.1
CDS	36..1517
Translation	MARAPLGVLLLLGLLGRGVGKNEELRLYHHLFNNYDPGSRPVREPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDWQDYRLNYSKDDFGGIETLRVPSELVWLPEIVLENNIDGQFGVAYDANVLVYEGGSVTWLPPAIYRSVCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFTFAVDNDGKTINKIDIDTEAYTENGEWAIDFCPGVIRRHHGGATDGPGETDVIYSLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRFLIFVMVVATLIVMNCVIVLNVSQRTPTTHAMSPRLRHVLLELLPRLLGSPPPPEAPRAASPPRRASSVGLLLRAEELILKKPRSELVFEGQRHRQGTWTAAFCQSLGAAAPEVRCCVDAVNFVAESTRDQEATGEEVSDWVRMGNALDNICFWAALVLFSVGSSLIFLGAYFNRVPDLPYAPCIQP

Target ORF information:

RefSeq Version	NM_000080.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic epsilon subunit (CHRNE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000080.4

ORF Insert Sequence:

ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG 
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC 
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG 
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC 
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC 
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT 
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG 
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT 
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC 
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG 
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCGGCGCA GGCCGGCGGC CAGAAATGCA CGGTCTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTCATT GCCCAGAAAA TCCCAGAGAC TTCTCTGAGC 
GTGCCGCTCC TGGGCAGGTT CCTTATTTTC GTCATGGTGG TCGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCCCAG CGGACGCCCA CCACCCACGC CATGTCCCCG 
CGGCTGCGCC ACGTTCTCCT GGAGCTGCTG CCGCGCCTCC TGGGCTCCCC GCCGCCGCCC 
GAGGCCCCCC GGGCCGCCTC GCCCCCAAGG CGGGCGTCGT CGGTGGGCTT ATTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCACGG AGCGAGCTCG TGTTTGAGGG GCAGAGGCAC 
CGGCAGGGGA CCTGGACGGC TGCCTTCTGC CAGAGCCTGG GCGCCGCCGC CCCCGAGGTC 
CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCCGAGAGCA CGAGAGATCA GGAGGCCACC 
GGCGAGGAAG TGTCCGACTG GGTGCGCATG GGGAATGCCC TTGACAACAT CTGCTTCTGG 
GCCGCTCTGG TGCTCTTCAG CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC 
CGAGTGCCTG ATCTCCCCTA CGCGCCGTGT ATCCAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNE ( NM_000080.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000071.1 Homo sapiens cholinergic receptor nicotinic epsilon subunit (CHRNE), mRNA.

Price & Availability↑

CloneID	OHu20760
Clone ID Related Accession (Same CDS sequence)	NM_000080.4 , NM_000080.3
Accession Version	NM_000080.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-12
Organism	Homo sapiens(human)
Product	acetylcholine receptor subunit epsilon precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X66403.1 and AC233723.2. This sequence is a reference standard in the RefSeqGene project. On Apr 20, 2010 this sequence version replaced NM_000080.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X66403.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159607 [ECO:0000348] ##Evidence-Data-END##

ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG 
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC 
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG 
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC 
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC 
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT 
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG 
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT 
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC 
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG 
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCGGCGCA GGCCGGCGGC CAGAAATGCA CGGTCTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTCATT GCCCAGAAAA TCCCAGAGAC TTCTCTGAGC 
GTGCCGCTCC TGGGCAGGTT CCTTATTTTC GTCATGGTGG TCGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCCCAG CGGACGCCCA CCACCCACGC CATGTCCCCG 
CGGCTGCGCC ACGTTCTCCT GGAGCTGCTG CCGCGCCTCC TGGGCTCCCC GCCGCCGCCC 
GAGGCCCCCC GGGCCGCCTC GCCCCCAAGG CGGGCGTCGT CGGTGGGCTT ATTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCACGG AGCGAGCTCG TGTTTGAGGG GCAGAGGCAC 
CGGCAGGGGA CCTGGACGGC TGCCTTCTGC CAGAGCCTGG GCGCCGCCGC CCCCGAGGTC 
CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCCGAGAGCA CGAGAGATCA GGAGGCCACC 
GGCGAGGAAG TGTCCGACTG GGTGCGCATG GGGAATGCCC TTGACAACAT CTGCTTCTGG 
GCCGCTCTGG TGCTCTTCAG CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC 
CGAGTGCCTG ATCTCCCCTA CGCGCCGTGT ATCCAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000071.1
CDS	12..1493
Translation	MARAPLGVLLLLGLLGRGVGKNEELRLYHHLFNNYDPGSRPVREPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDWQDYRLNYSKDDFGGIETLRVPSELVWLPEIVLENNIDGQFGVAYDANVLVYEGGSVTWLPPAIYRSVCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFTFAVDNDGKTINKIDIDTEAYTENGEWAIDFCPGVIRRHHGGATDGPGETDVIYSLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRFLIFVMVVATLIVMNCVIVLNVSQRTPTTHAMSPRLRHVLLELLPRLLGSPPPPEAPRAASPPRRASSVGLLLRAEELILKKPRSELVFEGQRHRQGTWTAAFCQSLGAAAPEVRCCVDAVNFVAESTRDQEATGEEVSDWVRMGNALDNICFWAALVLFSVGSSLIFLGAYFNRVPDLPYAPCIQP

Target ORF information:

RefSeq Version	NM_000080.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic epsilon subunit (CHRNE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000080.3

ORF Insert Sequence:

ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG 
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC 
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG 
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC 
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC 
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT 
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG 
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT 
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC 
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG 
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCGGCGCA GGCCGGCGGC CAGAAATGCA CGGTCTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTCATT GCCCAGAAAA TCCCAGAGAC TTCTCTGAGC 
GTGCCGCTCC TGGGCAGGTT CCTTATTTTC GTCATGGTGG TCGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCCCAG CGGACGCCCA CCACCCACGC CATGTCCCCG 
CGGCTGCGCC ACGTTCTCCT GGAGCTGCTG CCGCGCCTCC TGGGCTCCCC GCCGCCGCCC 
GAGGCCCCCC GGGCCGCCTC GCCCCCAAGG CGGGCGTCGT CGGTGGGCTT ATTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCACGG AGCGAGCTCG TGTTTGAGGG GCAGAGGCAC 
CGGCAGGGGA CCTGGACGGC TGCCTTCTGC CAGAGCCTGG GCGCCGCCGC CCCCGAGGTC 
CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCCGAGAGCA CGAGAGATCA GGAGGCCACC 
GGCGAGGAAG TGTCCGACTG GGTGCGCATG GGGAATGCCC TTGACAACAT CTGCTTCTGG 
GCCGCTCTGG TGCTCTTCAG CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC 
CGAGTGCCTG ATCTCCCCTA CGCGCCGTGT ATCCAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNE ( XM_017024115.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879604.1 Homo sapiens cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu86547
Clone ID Related Accession (Same CDS sequence)	XM_017024115.1
Accession Version	XM_017024115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	acetylcholine receptor subunit epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGAG GCAGGGGTGT GGGGAAGAAC GAGGAACTGC GTCTTTATCA CCATCTCTTC 
AACAACTATG ACCCAGGAAG CCGGCCAGTG CGGGAGCCTG AGGATACTGT CACCATCAGC 
CTCAAGGTCA CCCTGACGAA TCTCATCTCA CTGAATGAAA AAGAGGAGAC TCTCACCACT 
AGCGTCTGGA TTGGAATCGA TTGGCAGGAT TACCGACTCA ACTACAGCAA GGACGACTTT 
GGGGGTATAG AAACCCTGCG AGTCCCTTCA GAACTCGTGT GGCTGCCAGA GATTGTGCTG 
GAAAACAATA TTGATGGCCA GTTCGGAGTG GCCTACGACG CCAACGTGCT CGTCTACGAG 
GGCGGCTCCG TGACGTGGCT GCCTCCGGCC ATCTACCGCA GCGTCTGCGC AGTGGAGGTC 
ACCTACTTCC CCTTCGATTG GCAGAACTGT TCGCTTATTT TCCGCTCTCA GACGTACAAT 
GCCGAAGAGG TGGAGTTCAC TTTTGCCGTA GACAACGACG GCAAGACCAT CAACAAGATC 
GACATCGACA CAGAGGCCTA TACTGAGAAC GGCGAGTGGG CCATCGACTT CTGCCCGGGG 
GTGATCCGCC GCCACCACGG TGGCGCCACC GACGGCCCAG GGGAGACTGA CGTCATCTAC 
TCGCTCATCA TCCGCCGGAA GCCGCTCTTC TACGTCATTA ACATCATCGT GCCCTGTGTG 
CTCATCTCGG GCCTGGTGCT GCTCGCCTAC TTCCTGCCGG CGCAGGCCGG CGGCCAGAAA 
TGCACGGTCT CCATCAACGT CCTGCTCGCC CAGACCGTCT TCTTGTTCCT CATTGCCCAG 
AAAATCCCAG AGACTTCTCT GAGCGTGCCG CTCCTGGGCA GGTTCCTTAT TTTCGTCATG 
GTGGTCGCCA CGCTCATTGT CATGAATTGC GTCATCGTGC TCAACGTGTC CCAGCGGACG 
CCCACCACCC ACGCCATGTC CCCGCGGCTG CGCCACGTTC TCCTGGAGCT GCTGCCGCGC 
CTCCTGGGCT CCCCGCCGCC GCCCGAGGCC CCCCGGGCCG CCTCGCCCCC AAGGCGGGCG 
TCGTCGGTGG GCTTATTGCT CCGCGCGGAG GAGCTGATAC TGAAAAAGCC ACGGAGCGAG 
CTCGTGTTTG AGGGGCAGAG GCACCGGCAG GGGACCTGGA CGGCTGCCTT CTGCCAGAGC 
CTGGGCGCCG CCGCCCCCGA GGTCCGCTGC TGTGTGGATG CCGTGAACTT CGTGGCCGAG 
AGCACGAGAG ATCAGGAGGC CACCGGCGAG GAAGTGTCCG ACTGGGTGCG CATGGGGAAT 
GCCCTTGACA ACATCTGCTT CTGGGCCGCT CTGGTGCTCT TCAGCGTGGG CTCCAGCCTC 
ATCTTCCTCG GGGCCTACTT CAACCGAGTG CCTGATCTCC CCTACGCGCC GTGTATCCAG 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879604.1
CDS	67..1512
Translation	MQRGRGVGKNEELRLYHHLFNNYDPGSRPVREPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDWQDYRLNYSKDDFGGIETLRVPSELVWLPEIVLENNIDGQFGVAYDANVLVYEGGSVTWLPPAIYRSVCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFTFAVDNDGKTINKIDIDTEAYTENGEWAIDFCPGVIRRHHGGATDGPGETDVIYSLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRFLIFVMVVATLIVMNCVIVLNVSQRTPTTHAMSPRLRHVLLELLPRLLGSPPPPEAPRAASPPRRASSVGLLLRAEELILKKPRSELVFEGQRHRQGTWTAAFCQSLGAAAPEVRCCVDAVNFVAESTRDQEATGEEVSDWVRMGNALDNICFWAALVLFSVGSSLIFLGAYFNRVPDLPYAPCIQP

Target ORF information:

RefSeq Version	XM_017024115.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024115.1

ORF Insert Sequence:

ATGCAAAGAG GCAGGGGTGT GGGGAAGAAC GAGGAACTGC GTCTTTATCA CCATCTCTTC 
AACAACTATG ACCCAGGAAG CCGGCCAGTG CGGGAGCCTG AGGATACTGT CACCATCAGC 
CTCAAGGTCA CCCTGACGAA TCTCATCTCA CTGAATGAAA AAGAGGAGAC TCTCACCACT 
AGCGTCTGGA TTGGAATCGA TTGGCAGGAT TACCGACTCA ACTACAGCAA GGACGACTTT 
GGGGGTATAG AAACCCTGCG AGTCCCTTCA GAACTCGTGT GGCTGCCAGA GATTGTGCTG 
GAAAACAATA TTGATGGCCA GTTCGGAGTG GCCTACGACG CCAACGTGCT CGTCTACGAG 
GGCGGCTCCG TGACGTGGCT GCCTCCGGCC ATCTACCGCA GCGTCTGCGC AGTGGAGGTC 
ACCTACTTCC CCTTCGATTG GCAGAACTGT TCGCTTATTT TCCGCTCTCA GACGTACAAT 
GCCGAAGAGG TGGAGTTCAC TTTTGCCGTA GACAACGACG GCAAGACCAT CAACAAGATC 
GACATCGACA CAGAGGCCTA TACTGAGAAC GGCGAGTGGG CCATCGACTT CTGCCCGGGG 
GTGATCCGCC GCCACCACGG TGGCGCCACC GACGGCCCAG GGGAGACTGA CGTCATCTAC 
TCGCTCATCA TCCGCCGGAA GCCGCTCTTC TACGTCATTA ACATCATCGT GCCCTGTGTG 
CTCATCTCGG GCCTGGTGCT GCTCGCCTAC TTCCTGCCGG CGCAGGCCGG CGGCCAGAAA 
TGCACGGTCT CCATCAACGT CCTGCTCGCC CAGACCGTCT TCTTGTTCCT CATTGCCCAG 
AAAATCCCAG AGACTTCTCT GAGCGTGCCG CTCCTGGGCA GGTTCCTTAT TTTCGTCATG 
GTGGTCGCCA CGCTCATTGT CATGAATTGC GTCATCGTGC TCAACGTGTC CCAGCGGACG 
CCCACCACCC ACGCCATGTC CCCGCGGCTG CGCCACGTTC TCCTGGAGCT GCTGCCGCGC 
CTCCTGGGCT CCCCGCCGCC GCCCGAGGCC CCCCGGGCCG CCTCGCCCCC AAGGCGGGCG 
TCGTCGGTGG GCTTATTGCT CCGCGCGGAG GAGCTGATAC TGAAAAAGCC ACGGAGCGAG 
CTCGTGTTTG AGGGGCAGAG GCACCGGCAG GGGACCTGGA CGGCTGCCTT CTGCCAGAGC 
CTGGGCGCCG CCGCCCCCGA GGTCCGCTGC TGTGTGGATG CCGTGAACTT CGTGGCCGAG 
AGCACGAGAG ATCAGGAGGC CACCGGCGAG GAAGTGTCCG ACTGGGTGCG CATGGGGAAT 
GCCCTTGACA ACATCTGCTT CTGGGCCGCT CTGGTGCTCT TCAGCGTGGG CTCCAGCCTC 
ATCTTCCTCG GGGCCTACTT CAACCGAGTG CCTGATCTCC CCTACGCGCC GTGTATCCAG 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Congenital myasthenic syndrome in Israel: Genetic and clinical characterization.
Neuromuscular disorders : NMD27(2)136-140(2017 Feb)
Aharoni S,Sadeh M,Shapira Y,Edvardson S,Daana M,Dor-Wollman T,Mimouni-Bloch A,Halevy A,Cohen R,Sagie L,Argov Z,Rabie M,Spiegel R,Chervinsky I,Orenstein N,Engel AG,Nevo Y

Mutations Causing Slow-Channel Myasthenia Reveal That a Valine Ring in the Channel Pore of Muscle AChR is Optimized for Stabilizing Channel Gating.
Human mutation37(10)1051-9(2016 10)
Shen XM,Okuno T,Milone M,Otsuka K,Takahashi K,Komaki H,Giles E,Ohno K,Engel AG

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CHRNE cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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