CHRNB3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRNB3
Entrez Gene ID	1142
Full Name	cholinergic receptor nicotinic beta 3 subunit
General protein information	Preferred Names cholinergic receptor nicotinic beta 3 subunit Names neuronal acetylcholine receptor subunit beta-3 acetylcholine receptor, neuronal nicotinic, beta-3 subunit acetylcholine receptor, nicotinic, beta 3 (neuronal) cholinergic receptor, nicotinic beta 3 cholinergic receptor, nicotinic, beta 3 (neuronal) cholinergic receptor, nicotinic, beta polypeptide 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8p11.21
Summary	The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are (hetero)pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of nAChRs are encoded by separate genes and have different primary structure. There are several subtypes of neuronal nAChRs that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if, like muscle alpha-1 (MIM 100690), they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits (Groot Kormelink and Luyten, 1997 [PubMed 9009220]). Elliott et al. (1996) [PubMed 8906617] stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.[supplied by OMIM, Apr 2010].
Disorder MIM:	118508

mRNA and Protein(s)

mRNA	Protein	Name
NM_001347717.1	NP_001334646.1	neuronal acetylcholine receptor subunit beta-3 isoform 2
XM_011544390.1	XP_011542692.1	neuronal acetylcholine receptor subunit beta-3 isoform X1
NM_000749.3	NP_000740.1	neuronal acetylcholine receptor subunit beta-3 precursor
NM_000749.4	NP_000740.1	neuronal acetylcholine receptor subunit beta-3 isoform 1 precursor
NM_000749.5	NP_000740.1	neuronal acetylcholine receptor subunit beta-3 isoform 1 precursor
XM_011544390.2	XP_011542692.1	neuronal acetylcholine receptor subunit beta-3 isoform X1

Pathways from BioSystems

Homologies

	CHRNB3	XP_001115605.1
Bos taurus (cattle)	CHRNB3	XP_002698773.2
Rattus norvegicus (Norway rat)	Chrnb3	NP_598281.1
Gallus gallus (chicken)	CHRNB3	NP_990143.1
Danio rerio (zebrafish)	chrnb3b	NP_775394.1
Xenopus tropicalis (tropical clawed frog)	chrnb3	XP_002942943.1
Mus musculus (house mouse)	Chrnb3	NP_775304.1
Danio rerio (zebrafish)	chrnb3a	NP_957514.1
Homo sapiens (human)	CHRNB3	NP_000740.1
Pan troglodytes (chimpanzee)	CHRNB3	NP_001029105.1
Canis lupus familiaris (dog)	CHRNB3	XP_539952.3

Gene Ontology
Bibliography

Related articles in PubMed

Significant association of the CHRNB3-CHRNA6 gene cluster with nicotine dependence in the Chinese Han population.
Wen L, Han H, Liu Q, Su K, Yang Z, Cui W, Yuan W, Ma Y, Fan R, Chen J, Jiang K, Jiang X, Payne TJ, Wang J, Li MD
Scientific reports7(1)9745(2017 Aug)

Unorthodox Acetylcholine Binding Sites Formed by ?5 and ?3 Accessory Subunits in ?4?2* Nicotinic Acetylcholine Receptors.
Jain A, Kuryatov A, Wang J, Kamenecka TM, Lindstrom J
The Journal of biological chemistry291(45)23452-23463(2016 Nov)

Evidence for Association Between Low Frequency Variants in CHRNA6/CHRNB3 and Antisocial Drug Dependence.
Kamens HM, Corley RP, Richmond PA, Darlington TM, Dowell R, Hopfer CJ, Stallings MC, Hewitt JK, Brown SA, Ehringer MA
Behavior genetics46(5)693-704(2016 Sep)

Crucial roles of the CHRNB3-CHRNA6 gene cluster on chromosome 8 in nicotine dependence: update and subjects for future research.
Wen L, Yang Z, Cui W, Li MD
Translational psychiatry6(6)e843(2016 06)

The ?3 subunit of the nicotinic acetylcholine receptor: Modulation of gene expression and nicotine consumption.
Kamens HM, Miyamoto J, Powers MS, Ro K, Soto M, Cox R, Stitzel JA, Ehringer MA
Neuropharmacology99639-49(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study shows that unorthodox acetylcholine binding sites can form at the alpha5/alpha4 and beta3/alpha4 interfaces in (alpha4beta2)2alpha5 and (alpha4beta2)2beta3 nicotinic acetylcholine receptors (nAChRs) and at the alpha4/alpha5 in (beta2alpha4)2alpha5 nAChRs.
Title: Unorthodox Acetylcholine Binding Sites Formed by ?5 and ?3 Accessory Subunits in ?4?2* Nicotinic Acetylcholine Receptors.

More low frequency variants in the CHRNA6/CHRNB3 gene region were observed in cases compared to controls.
Title: Evidence for Association Between Low Frequency Variants in CHRNA6/CHRNB3 and Antisocial Drug Dependence.

To gain a better understanding of the pathological processes underlying ND and ND-related behaviors and to promote the development of effective smoking cessation therapies, we here present the most recent studies concerning the genetic effects of the CHRNB3-CHRNA6 gene cluster in ND.
Title: Crucial roles of the CHRNB3-CHRNA6 gene cluster on chromosome 8 in nicotine dependence: update and subjects for future research.

Data provide evidence that the protective genetic variant at rs6474413 identified in human genetic studies reduces gene expression and that decreased beta3 gene expression in mice reduces nicotine intake
Title: The ?3 subunit of the nicotinic acetylcholine receptor: Modulation of gene expression and nicotine consumption.

Polymorphism in CHRNB3 gene is associated with esophageal adenocarcinoma.
Title: A genetic variant in CHRNB3-CHRNA6 increases risk of esophageal squamous cell carcinoma in Chinese populations.

The following CHRNB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13307	NM_000749.5 Latest version!	Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu102288	NM_001347717.1 Latest version!	Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu56891	XM_011544390.2 Latest version!	Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu13307	NM_000749.3	Homo sapiens cholinergic receptor, nicotinic, beta 3 (neuronal) (CHRNB3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu13307	NM_000749.4	Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu56891	XM_011544390.1	Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRNB3 ( NM_000749.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000740.1 Homo sapiens cholinergic receptor, nicotinic, beta 3 (neuronal) (CHRNB3), mRNA.

Price & Availability↑

CloneID	OHu13307
Clone ID Related Accession (Same CDS sequence)	NM_000749.3 , NM_000749.4 , NM_000749.5
Accession Version	NM_000749.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	neuronal acetylcholine receptor subunit beta-3 precursor
Comment

ATGCTCCCAG ATTTTATGCT GGTTCTCATC GTCCTTGGCA TCCCTTCCTC AGCCACCACA 
GGTTTCAACT CAATCGCCGA AAATGAAGAT GCCCTCCTCA GACATTTGTT CCAAGGTTAT 
CAGAAATGGG TCCGCCCTGT ATTACATTCT AATGACACCA TAAAAGTATA TTTTGGATTG 
AAAATATCCC AGCTTGTAGA TGTGGATGAA AAGAATCAGC TGATGACAAC CAATGTGTGG 
CTCAAACAGG AATGGACAGA CCACAAGTTA CGCTGGAATC CTGATGATTA TGGTGGGATC 
CATTCCATTA AAGTTCCATC AGAATCTCTG TGGCTTCCTG ACATAGTTCT CTTTGAAAAT 
GCTGACGGCC GCTTCGAAGG CTCCCTGATG ACCAAGGTCA TCGTGAAATC AAACGGAACT 
GTTGTCTGGA CCCCTCCCGC CAGCTACAAA AGCTCCTGCA CCATGGACGT CACGTTTTTC 
CCGTTCGACC GACAGAACTG CTCCATGAAG TTTGGATCCT GGACTTATGA TGGCACCATG 
GTTGACCTCA TTTTGATCAA TGAAAATGTC GACAGAAAAG ACTTCTTCGA TAACGGAGAA 
TGGGAAATAC TGAACGCAAA GGGGATGAAG GGGAACAGAA GGGACGGCGT GTACTCCTAT 
CCCTTTATCA CGTATTCCTT CGTCCTGAGA CGCCTGCCTT TATTCTATAC CCTCTTTCTC 
ATCATCCCCT GCCTGGGGCT GTCTTTCCTA ACAGTTCTTG TGTTCTATTT ACCTTCGGAT 
GAAGGAGAAA AACTTTCATT ATCCACATCG GTCTTGGTTT CTCTGACAGT TTTCCTTTTA 
GTGATTGAAG AAATCATCCC ATCGTCTTCC AAAGTCATTC CTCTCATTGG AGAGTACCTG 
CTGTTCATCA TGATTTTTGT GACCCTGTCC ATCATTGTTA CCGTGTTTGT CATTAACGTT 
CACCACAGAT CTTCTTCCAC GTACCACCCC ATGGCCCCCT GGGTTAAGAG GCTCTTTCTG 
CAGAAACTTC CAAAATTACT TTGCATGAAA GATCATGTGG ATCGCTACTC ATCCCCAGAG 
AAAGAGGAGA GTCAACCAGT AGTGAAAGGC AAAGTCCTCG AAAAAAAGAA ACAGAAACAG 
CTTAGTGATG GAGAAAAAGT TCTAGTTGCT TTTTTGGAAA AAGCTGCTGA TTCCATTAGA 
TACATTTCGA GACATGTGAA GAAAGAACAT TTTATCAGCC AGGTAGTACA AGACTGGAAA 
TTTGTAGCTC AAGTTCTTGA CCGAATCTTC CTGTGGCTCT TTCTGATAGT GTCAGTAACA 
GGCTCGGTTC TGATTTTTAC CCCTGCTTTG AAGATGTGGC TACATAGTTA CCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_000740.1

Translation

MLPDFMLVLIVLGIPSSATTGFNSIAENEDALLRHLFQGYQKWVRPVLHSNDTIKVYFGLKISQLVDVDEKNQLMTTNVWLKQEWTDHKLRWNPDDYGGIHSIKVPSESLWLPDIVLFENADGRFEGSLMTKVIVKSNGTVVWTPPASYKSSCTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLILINENVDRKDFFDNGEWEILNAKGMKGNRRDGVYSYPFITYSFVLRRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSSTYHPMAPWVKRLFLQKLPKLLCMKDHVDRYSSPEKEESQPVVKGKVLEKKKQKQLSDGEKVLVAFLEKAADSIRYISRHVKKEHFISQVVQDWKFVAQVLDRIFLWLFLIVSVTGSVLIFTPALKMWLHSYH

Target ORF information:

RefSeq Version	NM_000749.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens cholinergic receptor, nicotinic, beta 3 (neuronal) (CHRNB3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000749.3

ORF Insert Sequence:

ATGCTCCCAG ATTTTATGCT GGTTCTCATC GTCCTTGGCA TCCCTTCCTC AGCCACCACA 
GGTTTCAACT CAATCGCCGA AAATGAAGAT GCCCTCCTCA GACATTTGTT CCAAGGTTAT 
CAGAAATGGG TCCGCCCTGT ATTACATTCT AATGACACCA TAAAAGTATA TTTTGGATTG 
AAAATATCCC AGCTTGTAGA TGTGGATGAA AAGAATCAGC TGATGACAAC CAATGTGTGG 
CTCAAACAGG AATGGACAGA CCACAAGTTA CGCTGGAATC CTGATGATTA TGGTGGGATC 
CATTCCATTA AAGTTCCATC AGAATCTCTG TGGCTTCCTG ACATAGTTCT CTTTGAAAAT 
GCTGACGGCC GCTTCGAAGG CTCCCTGATG ACCAAGGTCA TCGTGAAATC AAACGGAACT 
GTTGTCTGGA CCCCTCCCGC CAGCTACAAA AGCTCCTGCA CCATGGACGT CACGTTTTTC 
CCGTTCGACC GACAGAACTG CTCCATGAAG TTTGGATCCT GGACTTATGA TGGCACCATG 
GTTGACCTCA TTTTGATCAA TGAAAATGTC GACAGAAAAG ACTTCTTCGA TAACGGAGAA 
TGGGAAATAC TGAACGCAAA GGGGATGAAG GGGAACAGAA GGGACGGCGT GTACTCCTAT 
CCCTTTATCA CGTATTCCTT CGTCCTGAGA CGCCTGCCTT TATTCTATAC CCTCTTTCTC 
ATCATCCCCT GCCTGGGGCT GTCTTTCCTA ACAGTTCTTG TGTTCTATTT ACCTTCGGAT 
GAAGGAGAAA AACTTTCATT ATCCACATCG GTCTTGGTTT CTCTGACAGT TTTCCTTTTA 
GTGATTGAAG AAATCATCCC ATCGTCTTCC AAAGTCATTC CTCTCATTGG AGAGTACCTG 
CTGTTCATCA TGATTTTTGT GACCCTGTCC ATCATTGTTA CCGTGTTTGT CATTAACGTT 
CACCACAGAT CTTCTTCCAC GTACCACCCC ATGGCCCCCT GGGTTAAGAG GCTCTTTCTG 
CAGAAACTTC CAAAATTACT TTGCATGAAA GATCATGTGG ATCGCTACTC ATCCCCAGAG 
AAAGAGGAGA GTCAACCAGT AGTGAAAGGC AAAGTCCTCG AAAAAAAGAA ACAGAAACAG 
CTTAGTGATG GAGAAAAAGT TCTAGTTGCT TTTTTGGAAA AAGCTGCTGA TTCCATTAGA 
TACATTTCGA GACATGTGAA GAAAGAACAT TTTATCAGCC AGGTAGTACA AGACTGGAAA 
TTTGTAGCTC AAGTTCTTGA CCGAATCTTC CTGTGGCTCT TTCTGATAGT GTCAGTAACA 
GGCTCGGTTC TGATTTTTAC CCCTGCTTTG AAGATGTGGC TACATAGTTA CCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNB3 ( NM_000749.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000740.1 Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13307
Clone ID Related Accession (Same CDS sequence)	NM_000749.3 , NM_000749.4 , NM_000749.5
Accession Version	NM_000749.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	neuronal acetylcholine receptor subunit beta-3 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from Y08417.1, U62438.1, BU736120.1 and AC087533.7. On Dec 10, 2016 this sequence version replaced NM_000749.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y08417.1, SRR1803612.105406.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402 [ECO:0000348] ##Evidence-Data-END##

ATGCTCCCAG ATTTTATGCT GGTTCTCATC GTCCTTGGCA TCCCTTCCTC AGCCACCACA 
GGTTTCAACT CAATCGCCGA AAATGAAGAT GCCCTCCTCA GACATTTGTT CCAAGGTTAT 
CAGAAATGGG TCCGCCCTGT ATTACATTCT AATGACACCA TAAAAGTATA TTTTGGATTG 
AAAATATCCC AGCTTGTAGA TGTGGATGAA AAGAATCAGC TGATGACAAC CAATGTGTGG 
CTCAAACAGG AATGGACAGA CCACAAGTTA CGCTGGAATC CTGATGATTA TGGTGGGATC 
CATTCCATTA AAGTTCCATC AGAATCTCTG TGGCTTCCTG ACATAGTTCT CTTTGAAAAT 
GCTGACGGCC GCTTCGAAGG CTCCCTGATG ACCAAGGTCA TCGTGAAATC AAACGGAACT 
GTTGTCTGGA CCCCTCCCGC CAGCTACAAA AGCTCCTGCA CCATGGACGT CACGTTTTTC 
CCGTTCGACC GACAGAACTG CTCCATGAAG TTTGGATCCT GGACTTATGA TGGCACCATG 
GTTGACCTCA TTTTGATCAA TGAAAATGTC GACAGAAAAG ACTTCTTCGA TAACGGAGAA 
TGGGAAATAC TGAACGCAAA GGGGATGAAG GGGAACAGAA GGGACGGCGT GTACTCCTAT 
CCCTTTATCA CGTATTCCTT CGTCCTGAGA CGCCTGCCTT TATTCTATAC CCTCTTTCTC 
ATCATCCCCT GCCTGGGGCT GTCTTTCCTA ACAGTTCTTG TGTTCTATTT ACCTTCGGAT 
GAAGGAGAAA AACTTTCATT ATCCACATCG GTCTTGGTTT CTCTGACAGT TTTCCTTTTA 
GTGATTGAAG AAATCATCCC ATCGTCTTCC AAAGTCATTC CTCTCATTGG AGAGTACCTG 
CTGTTCATCA TGATTTTTGT GACCCTGTCC ATCATTGTTA CCGTGTTTGT CATTAACGTT 
CACCACAGAT CTTCTTCCAC GTACCACCCC ATGGCCCCCT GGGTTAAGAG GCTCTTTCTG 
CAGAAACTTC CAAAATTACT TTGCATGAAA GATCATGTGG ATCGCTACTC ATCCCCAGAG 
AAAGAGGAGA GTCAACCAGT AGTGAAAGGC AAAGTCCTCG AAAAAAAGAA ACAGAAACAG 
CTTAGTGATG GAGAAAAAGT TCTAGTTGCT TTTTTGGAAA AAGCTGCTGA TTCCATTAGA 
TACATTTCGA GACATGTGAA GAAAGAACAT TTTATCAGCC AGGTAGTACA AGACTGGAAA 
TTTGTAGCTC AAGTTCTTGA CCGAATCTTC CTGTGGCTCT TTCTGATAGT GTCAGTAACA 
GGCTCGGTTC TGATTTTTAC CCCTGCTTTG AAGATGTGGC TACATAGTTA CCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000740.1
CDS	129..1505
Translation	MLPDFMLVLIVLGIPSSATTGFNSIAENEDALLRHLFQGYQKWVRPVLHSNDTIKVYFGLKISQLVDVDEKNQLMTTNVWLKQEWTDHKLRWNPDDYGGIHSIKVPSESLWLPDIVLFENADGRFEGSLMTKVIVKSNGTVVWTPPASYKSSCTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLILINENVDRKDFFDNGEWEILNAKGMKGNRRDGVYSYPFITYSFVLRRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSSTYHPMAPWVKRLFLQKLPKLLCMKDHVDRYSSPEKEESQPVVKGKVLEKKKQKQLSDGEKVLVAFLEKAADSIRYISRHVKKEHFISQVVQDWKFVAQVLDRIFLWLFLIVSVTGSVLIFTPALKMWLHSYH

Target ORF information:

RefSeq Version	NM_000749.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000749.4

ORF Insert Sequence:

ATGCTCCCAG ATTTTATGCT GGTTCTCATC GTCCTTGGCA TCCCTTCCTC AGCCACCACA 
GGTTTCAACT CAATCGCCGA AAATGAAGAT GCCCTCCTCA GACATTTGTT CCAAGGTTAT 
CAGAAATGGG TCCGCCCTGT ATTACATTCT AATGACACCA TAAAAGTATA TTTTGGATTG 
AAAATATCCC AGCTTGTAGA TGTGGATGAA AAGAATCAGC TGATGACAAC CAATGTGTGG 
CTCAAACAGG AATGGACAGA CCACAAGTTA CGCTGGAATC CTGATGATTA TGGTGGGATC 
CATTCCATTA AAGTTCCATC AGAATCTCTG TGGCTTCCTG ACATAGTTCT CTTTGAAAAT 
GCTGACGGCC GCTTCGAAGG CTCCCTGATG ACCAAGGTCA TCGTGAAATC AAACGGAACT 
GTTGTCTGGA CCCCTCCCGC CAGCTACAAA AGCTCCTGCA CCATGGACGT CACGTTTTTC 
CCGTTCGACC GACAGAACTG CTCCATGAAG TTTGGATCCT GGACTTATGA TGGCACCATG 
GTTGACCTCA TTTTGATCAA TGAAAATGTC GACAGAAAAG ACTTCTTCGA TAACGGAGAA 
TGGGAAATAC TGAACGCAAA GGGGATGAAG GGGAACAGAA GGGACGGCGT GTACTCCTAT 
CCCTTTATCA CGTATTCCTT CGTCCTGAGA CGCCTGCCTT TATTCTATAC CCTCTTTCTC 
ATCATCCCCT GCCTGGGGCT GTCTTTCCTA ACAGTTCTTG TGTTCTATTT ACCTTCGGAT 
GAAGGAGAAA AACTTTCATT ATCCACATCG GTCTTGGTTT CTCTGACAGT TTTCCTTTTA 
GTGATTGAAG AAATCATCCC ATCGTCTTCC AAAGTCATTC CTCTCATTGG AGAGTACCTG 
CTGTTCATCA TGATTTTTGT GACCCTGTCC ATCATTGTTA CCGTGTTTGT CATTAACGTT 
CACCACAGAT CTTCTTCCAC GTACCACCCC ATGGCCCCCT GGGTTAAGAG GCTCTTTCTG 
CAGAAACTTC CAAAATTACT TTGCATGAAA GATCATGTGG ATCGCTACTC ATCCCCAGAG 
AAAGAGGAGA GTCAACCAGT AGTGAAAGGC AAAGTCCTCG AAAAAAAGAA ACAGAAACAG 
CTTAGTGATG GAGAAAAAGT TCTAGTTGCT TTTTTGGAAA AAGCTGCTGA TTCCATTAGA 
TACATTTCGA GACATGTGAA GAAAGAACAT TTTATCAGCC AGGTAGTACA AGACTGGAAA 
TTTGTAGCTC AAGTTCTTGA CCGAATCTTC CTGTGGCTCT TTCTGATAGT GTCAGTAACA 
GGCTCGGTTC TGATTTTTAC CCCTGCTTTG AAGATGTGGC TACATAGTTA CCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNB3 ( NM_000749.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000740.1 Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13307
Clone ID Related Accession (Same CDS sequence)	NM_000749.3 , NM_000749.4 , NM_000749.5
Accession Version	NM_000749.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	neuronal acetylcholine receptor subunit beta-3 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC090739.13, AC103843.3 and AC087533.7. On May 17, 2019 this sequence version replaced NM_000749.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.105406.1, SRR1803616.72644.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000289957.3/ ENSP00000289957.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGCTCCCAG ATTTTATGCT GGTTCTCATC GTCCTTGGCA TCCCTTCCTC AGCCACCACA 
GGTTTCAACT CAATCGCCGA AAATGAAGAT GCCCTCCTCA GACATTTGTT CCAAGGTTAT 
CAGAAATGGG TCCGCCCTGT ATTACATTCT AATGACACCA TAAAAGTATA TTTTGGATTG 
AAAATATCCC AGCTTGTAGA TGTGGATGAA AAGAATCAGC TGATGACAAC CAATGTGTGG 
CTCAAACAGG AATGGACAGA CCACAAGTTA CGCTGGAATC CTGATGATTA TGGTGGGATC 
CATTCCATTA AAGTTCCATC AGAATCTCTG TGGCTTCCTG ACATAGTTCT CTTTGAAAAT 
GCTGACGGCC GCTTCGAAGG CTCCCTGATG ACCAAGGTCA TCGTGAAATC AAACGGAACT 
GTTGTCTGGA CCCCTCCCGC CAGCTACAAA AGCTCCTGCA CCATGGACGT CACGTTTTTC 
CCGTTCGACC GACAGAACTG CTCCATGAAG TTTGGATCCT GGACTTATGA TGGCACCATG 
GTTGACCTCA TTTTGATCAA TGAAAATGTC GACAGAAAAG ACTTCTTCGA TAACGGAGAA 
TGGGAAATAC TGAACGCAAA GGGGATGAAG GGGAACAGAA GGGACGGCGT GTACTCCTAT 
CCCTTTATCA CGTATTCCTT CGTCCTGAGA CGCCTGCCTT TATTCTATAC CCTCTTTCTC 
ATCATCCCCT GCCTGGGGCT GTCTTTCCTA ACAGTTCTTG TGTTCTATTT ACCTTCGGAT 
GAAGGAGAAA AACTTTCATT ATCCACATCG GTCTTGGTTT CTCTGACAGT TTTCCTTTTA 
GTGATTGAAG AAATCATCCC ATCGTCTTCC AAAGTCATTC CTCTCATTGG AGAGTACCTG 
CTGTTCATCA TGATTTTTGT GACCCTGTCC ATCATTGTTA CCGTGTTTGT CATTAACGTT 
CACCACAGAT CTTCTTCCAC GTACCACCCC ATGGCCCCCT GGGTTAAGAG GCTCTTTCTG 
CAGAAACTTC CAAAATTACT TTGCATGAAA GATCATGTGG ATCGCTACTC ATCCCCAGAG 
AAAGAGGAGA GTCAACCAGT AGTGAAAGGC AAAGTCCTCG AAAAAAAGAA ACAGAAACAG 
CTTAGTGATG GAGAAAAAGT TCTAGTTGCT TTTTTGGAAA AAGCTGCTGA TTCCATTAGA 
TACATTTCGA GACATGTGAA GAAAGAACAT TTTATCAGCC AGGTAGTACA AGACTGGAAA 
TTTGTAGCTC AAGTTCTTGA CCGAATCTTC CTGTGGCTCT TTCTGATAGT GTCAGTAACA 
GGCTCGGTTC TGATTTTTAC CCCTGCTTTG AAGATGTGGC TACATAGTTA CCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000740.1
CDS	182..1558
Translation	MLPDFMLVLIVLGIPSSATTGFNSIAENEDALLRHLFQGYQKWVRPVLHSNDTIKVYFGLKISQLVDVDEKNQLMTTNVWLKQEWTDHKLRWNPDDYGGIHSIKVPSESLWLPDIVLFENADGRFEGSLMTKVIVKSNGTVVWTPPASYKSSCTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLILINENVDRKDFFDNGEWEILNAKGMKGNRRDGVYSYPFITYSFVLRRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSSTYHPMAPWVKRLFLQKLPKLLCMKDHVDRYSSPEKEESQPVVKGKVLEKKKQKQLSDGEKVLVAFLEKAADSIRYISRHVKKEHFISQVVQDWKFVAQVLDRIFLWLFLIVSVTGSVLIFTPALKMWLHSYH

Target ORF information:

RefSeq Version	NM_000749.5
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000749.5

ORF Insert Sequence:

ATGCTCCCAG ATTTTATGCT GGTTCTCATC GTCCTTGGCA TCCCTTCCTC AGCCACCACA 
GGTTTCAACT CAATCGCCGA AAATGAAGAT GCCCTCCTCA GACATTTGTT CCAAGGTTAT 
CAGAAATGGG TCCGCCCTGT ATTACATTCT AATGACACCA TAAAAGTATA TTTTGGATTG 
AAAATATCCC AGCTTGTAGA TGTGGATGAA AAGAATCAGC TGATGACAAC CAATGTGTGG 
CTCAAACAGG AATGGACAGA CCACAAGTTA CGCTGGAATC CTGATGATTA TGGTGGGATC 
CATTCCATTA AAGTTCCATC AGAATCTCTG TGGCTTCCTG ACATAGTTCT CTTTGAAAAT 
GCTGACGGCC GCTTCGAAGG CTCCCTGATG ACCAAGGTCA TCGTGAAATC AAACGGAACT 
GTTGTCTGGA CCCCTCCCGC CAGCTACAAA AGCTCCTGCA CCATGGACGT CACGTTTTTC 
CCGTTCGACC GACAGAACTG CTCCATGAAG TTTGGATCCT GGACTTATGA TGGCACCATG 
GTTGACCTCA TTTTGATCAA TGAAAATGTC GACAGAAAAG ACTTCTTCGA TAACGGAGAA 
TGGGAAATAC TGAACGCAAA GGGGATGAAG GGGAACAGAA GGGACGGCGT GTACTCCTAT 
CCCTTTATCA CGTATTCCTT CGTCCTGAGA CGCCTGCCTT TATTCTATAC CCTCTTTCTC 
ATCATCCCCT GCCTGGGGCT GTCTTTCCTA ACAGTTCTTG TGTTCTATTT ACCTTCGGAT 
GAAGGAGAAA AACTTTCATT ATCCACATCG GTCTTGGTTT CTCTGACAGT TTTCCTTTTA 
GTGATTGAAG AAATCATCCC ATCGTCTTCC AAAGTCATTC CTCTCATTGG AGAGTACCTG 
CTGTTCATCA TGATTTTTGT GACCCTGTCC ATCATTGTTA CCGTGTTTGT CATTAACGTT 
CACCACAGAT CTTCTTCCAC GTACCACCCC ATGGCCCCCT GGGTTAAGAG GCTCTTTCTG 
CAGAAACTTC CAAAATTACT TTGCATGAAA GATCATGTGG ATCGCTACTC ATCCCCAGAG 
AAAGAGGAGA GTCAACCAGT AGTGAAAGGC AAAGTCCTCG AAAAAAAGAA ACAGAAACAG 
CTTAGTGATG GAGAAAAAGT TCTAGTTGCT TTTTTGGAAA AAGCTGCTGA TTCCATTAGA 
TACATTTCGA GACATGTGAA GAAAGAACAT TTTATCAGCC AGGTAGTACA AGACTGGAAA 
TTTGTAGCTC AAGTTCTTGA CCGAATCTTC CTGTGGCTCT TTCTGATAGT GTCAGTAACA 
GGCTCGGTTC TGATTTTTAC CCCTGCTTTG AAGATGTGGC TACATAGTTA CCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNB3 ( NM_001347717.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001334646.1 Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant 2, mRNA.

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CloneID	OHu102288
Clone ID Related Accession (Same CDS sequence)	NM_001347717.1
Accession Version	NM_001347717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	neuronal acetylcholine receptor subunit beta-3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC090739.13, AC103843.3, AC087533.7 and BU736120.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.94669.1, SRR1803615.192086.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402 [ECO:0000348] ##Evidence-Data-END##

ATGACAACCA ATGTGTGGCT CAAACAGGAA TGGACAGACC ACAAGTTACG CTGGAATCCT 
GATGATTATG GTGGGATCCA TTCCATTAAA GTTCCATCAG AATCTCTGTG GCTTCCTGAC 
ATAGTTCTCT TTGAAAATGC TGACGGCCGC TTCGAAGGCT CCCTGATGAC CAAGGTCATC 
GTGAAATCAA ACGGAACTGT TGTCTGGACC CCTCCCGCCA GCTACAAAAG CTCCTGCACC 
ATGGACGTCA CGTTTTTCCC GTTCGACCGA CAGAACTGCT CCATGAAGTT TGGATCCTGG 
ACTTATGATG GCACCATGGT TGACCTCATT TTGATCAATG AAAATGTCGA CAGAAAAGAC 
TTCTTCGATA ACGGAGAATG GGAAATACTG AACGCAAAGG GGATGAAGGG GAACAGAAGG 
GACGGCGTGT ACTCCTATCC CTTTATCACG TATTCCTTCG TCCTGAGACG CCTGCCTTTA 
TTCTATACCC TCTTTCTCAT CATCCCCTGC CTGGGGCTGT CTTTCCTAAC AGTTCTTGTG 
TTCTATTTAC CTTCGGATGA AGGAGAAAAA CTTTCATTAT CCACATCGGT CTTGGTTTCT 
CTGACAGTTT TCCTTTTAGT GATTGAAGAA ATCATCCCAT CGTCTTCCAA AGTCATTCCT 
CTCATTGGAG AGTACCTGCT GTTCATCATG ATTTTTGTGA CCCTGTCCAT CATTGTTACC 
GTGTTTGTCA TTAACGTTCA CCACAGATCT TCTTCCACGT ACCACCCCAT GGCCCCCTGG 
GTTAAGAGGC TCTTTCTGCA GAAACTTCCA AAATTACTTT GCATGAAAGA TCATGTGGAT 
CGCTACTCAT CCCCAGAGAA AGAGGAGAGT CAACCAGTAG TGAAAGGCAA AGTCCTCGAA 
AAAAAGAAAC AGAAACAGCT TAGTGATGGA GAAAAAGTTC TAGTTGCTTT TTTGGAAAAA 
GCTGCTGATT CCATTAGATA CATTTCGAGA CATGTGAAGA AAGAACATTT TATCAGCCAG 
GTAGTACAAG ACTGGAAATT TGTAGCTCAA GTTCTTGACC GAATCTTCCT GTGGCTCTTT 
CTGATAGTGT CAGTAACAGG CTCGGTTCTG ATTTTTACCC CTGCTTTGAA GATGTGGCTA 
CATAGTTACC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334646.1
CDS	527..1681
Translation	MTTNVWLKQEWTDHKLRWNPDDYGGIHSIKVPSESLWLPDIVLFENADGRFEGSLMTKVIVKSNGTVVWTPPASYKSSCTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLILINENVDRKDFFDNGEWEILNAKGMKGNRRDGVYSYPFITYSFVLRRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSSTYHPMAPWVKRLFLQKLPKLLCMKDHVDRYSSPEKEESQPVVKGKVLEKKKQKQLSDGEKVLVAFLEKAADSIRYISRHVKKEHFISQVVQDWKFVAQVLDRIFLWLFLIVSVTGSVLIFTPALKMWLHSYH

Target ORF information:

RefSeq Version	NM_001347717.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347717.1

ORF Insert Sequence:

ATGACAACCA ATGTGTGGCT CAAACAGGAA TGGACAGACC ACAAGTTACG CTGGAATCCT 
GATGATTATG GTGGGATCCA TTCCATTAAA GTTCCATCAG AATCTCTGTG GCTTCCTGAC 
ATAGTTCTCT TTGAAAATGC TGACGGCCGC TTCGAAGGCT CCCTGATGAC CAAGGTCATC 
GTGAAATCAA ACGGAACTGT TGTCTGGACC CCTCCCGCCA GCTACAAAAG CTCCTGCACC 
ATGGACGTCA CGTTTTTCCC GTTCGACCGA CAGAACTGCT CCATGAAGTT TGGATCCTGG 
ACTTATGATG GCACCATGGT TGACCTCATT TTGATCAATG AAAATGTCGA CAGAAAAGAC 
TTCTTCGATA ACGGAGAATG GGAAATACTG AACGCAAAGG GGATGAAGGG GAACAGAAGG 
GACGGCGTGT ACTCCTATCC CTTTATCACG TATTCCTTCG TCCTGAGACG CCTGCCTTTA 
TTCTATACCC TCTTTCTCAT CATCCCCTGC CTGGGGCTGT CTTTCCTAAC AGTTCTTGTG 
TTCTATTTAC CTTCGGATGA AGGAGAAAAA CTTTCATTAT CCACATCGGT CTTGGTTTCT 
CTGACAGTTT TCCTTTTAGT GATTGAAGAA ATCATCCCAT CGTCTTCCAA AGTCATTCCT 
CTCATTGGAG AGTACCTGCT GTTCATCATG ATTTTTGTGA CCCTGTCCAT CATTGTTACC 
GTGTTTGTCA TTAACGTTCA CCACAGATCT TCTTCCACGT ACCACCCCAT GGCCCCCTGG 
GTTAAGAGGC TCTTTCTGCA GAAACTTCCA AAATTACTTT GCATGAAAGA TCATGTGGAT 
CGCTACTCAT CCCCAGAGAA AGAGGAGAGT CAACCAGTAG TGAAAGGCAA AGTCCTCGAA 
AAAAAGAAAC AGAAACAGCT TAGTGATGGA GAAAAAGTTC TAGTTGCTTT TTTGGAAAAA 
GCTGCTGATT CCATTAGATA CATTTCGAGA CATGTGAAGA AAGAACATTT TATCAGCCAG 
GTAGTACAAG ACTGGAAATT TGTAGCTCAA GTTCTTGACC GAATCTTCCT GTGGCTCTTT 
CTGATAGTGT CAGTAACAGG CTCGGTTCTG ATTTTTACCC CTGCTTTGAA GATGTGGCTA 
CATAGTTACC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNB3 ( XM_011544390.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542692.1 Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant X1, mRNA.

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CloneID	OHu56891
Clone ID Related Accession (Same CDS sequence)	XM_011544390.1 , XM_011544390.2
Accession Version	XM_011544390.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neuronal acetylcholine receptor subunit beta-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGG TCATCGTGAA ATCAAACGGA ACTGTTGTCT GGACCCCTCC CGCCAGCTAC 
AAAAGCTCCT GCACCATGGA CGTCACGTTT TTCCCGTTCG ACCGACAGAA CTGCTCCATG 
AAGTTTGGAT CCTGGACTTA TGATGGCACC ATGGTTGACC TCATTTTGAT CAATGAAAAT 
GTCGACAGAA AAGACTTCTT CGATAACGGA GAATGGGAAA TACTGAACGC AAAGGGGATG 
AAGGGGAACA GAAGGGACGG CGTGTACTCC TATCCCTTTA TCACGTATTC CTTCGTCCTG 
AGACGCCTGC CTTTATTCTA TACCCTCTTT CTCATCATCC CCTGCCTGGG GCTGTCTTTC 
CTAACAGTTC TTGTGTTCTA TTTACCTTCG GATGAAGGAG AAAAACTTTC ATTATCCACA 
TCGGTCTTGG TTTCTCTGAC AGTTTTCCTT TTAGTGATTG AAGAAATCAT CCCATCGTCT 
TCCAAAGTCA TTCCTCTCAT TGGAGAGTAC CTGCTGTTCA TCATGATTTT TGTGACCCTG 
TCCATCATTG TTACCGTGTT TGTCATTAAC GTTCACCACA GATCTTCTTC CACGTACCAC 
CCCATGGCCC CCTGGGTTAA GAGGCTCTTT CTGCAGAAAC TTCCAAAATT ACTTTGCATG 
AAAGATCATG TGGATCGCTA CTCATCCCCA GAGAAAGAGG AGAGTCAACC AGTAGTGAAA 
GGCAAAGTCC TCGAAAAAAA GAAACAGAAA CAGCTTAGTG ATGGAGAAAA AGTTCTAGTT 
GCTTTTTTGG AAAAAGCTGC TGATTCCATT AGATACATTT CGAGACATGT GAAGAAAGAA 
CATTTTATCA GCCAGGTAGT ACAAGACTGG AAATTTGTAG CTCAAGTTCT TGACCGAATC 
TTCCTGTGGC TCTTTCTGAT AGTGTCAGTA ACAGGCTCGG TTCTGATTTT TACCCCTGCT 
TTGAAGATGT GGCTACATAG TTACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542692.1
CDS	339..1328
Translation	MTKVIVKSNGTVVWTPPASYKSSCTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLILINENVDRKDFFDNGEWEILNAKGMKGNRRDGVYSYPFITYSFVLRRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSSTYHPMAPWVKRLFLQKLPKLLCMKDHVDRYSSPEKEESQPVVKGKVLEKKKQKQLSDGEKVLVAFLEKAADSIRYISRHVKKEHFISQVVQDWKFVAQVLDRIFLWLFLIVSVTGSVLIFTPALKMWLHSYH

Target ORF information:

RefSeq Version	XM_011544390.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544390.1

ORF Insert Sequence:

ATGACCAAGG TCATCGTGAA ATCAAACGGA ACTGTTGTCT GGACCCCTCC CGCCAGCTAC 
AAAAGCTCCT GCACCATGGA CGTCACGTTT TTCCCGTTCG ACCGACAGAA CTGCTCCATG 
AAGTTTGGAT CCTGGACTTA TGATGGCACC ATGGTTGACC TCATTTTGAT CAATGAAAAT 
GTCGACAGAA AAGACTTCTT CGATAACGGA GAATGGGAAA TACTGAACGC AAAGGGGATG 
AAGGGGAACA GAAGGGACGG CGTGTACTCC TATCCCTTTA TCACGTATTC CTTCGTCCTG 
AGACGCCTGC CTTTATTCTA TACCCTCTTT CTCATCATCC CCTGCCTGGG GCTGTCTTTC 
CTAACAGTTC TTGTGTTCTA TTTACCTTCG GATGAAGGAG AAAAACTTTC ATTATCCACA 
TCGGTCTTGG TTTCTCTGAC AGTTTTCCTT TTAGTGATTG AAGAAATCAT CCCATCGTCT 
TCCAAAGTCA TTCCTCTCAT TGGAGAGTAC CTGCTGTTCA TCATGATTTT TGTGACCCTG 
TCCATCATTG TTACCGTGTT TGTCATTAAC GTTCACCACA GATCTTCTTC CACGTACCAC 
CCCATGGCCC CCTGGGTTAA GAGGCTCTTT CTGCAGAAAC TTCCAAAATT ACTTTGCATG 
AAAGATCATG TGGATCGCTA CTCATCCCCA GAGAAAGAGG AGAGTCAACC AGTAGTGAAA 
GGCAAAGTCC TCGAAAAAAA GAAACAGAAA CAGCTTAGTG ATGGAGAAAA AGTTCTAGTT 
GCTTTTTTGG AAAAAGCTGC TGATTCCATT AGATACATTT CGAGACATGT GAAGAAAGAA 
CATTTTATCA GCCAGGTAGT ACAAGACTGG AAATTTGTAG CTCAAGTTCT TGACCGAATC 
TTCCTGTGGC TCTTTCTGAT AGTGTCAGTA ACAGGCTCGG TTCTGATTTT TACCCCTGCT 
TTGAAGATGT GGCTACATAG TTACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNB3 ( XM_011544390.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542692.1 Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu56891
Clone ID Related Accession (Same CDS sequence)	XM_011544390.1 , XM_011544390.2
Accession Version	XM_011544390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neuronal acetylcholine receptor subunit beta-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011544390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAAGG TCATCGTGAA ATCAAACGGA ACTGTTGTCT GGACCCCTCC CGCCAGCTAC 
AAAAGCTCCT GCACCATGGA CGTCACGTTT TTCCCGTTCG ACCGACAGAA CTGCTCCATG 
AAGTTTGGAT CCTGGACTTA TGATGGCACC ATGGTTGACC TCATTTTGAT CAATGAAAAT 
GTCGACAGAA AAGACTTCTT CGATAACGGA GAATGGGAAA TACTGAACGC AAAGGGGATG 
AAGGGGAACA GAAGGGACGG CGTGTACTCC TATCCCTTTA TCACGTATTC CTTCGTCCTG 
AGACGCCTGC CTTTATTCTA TACCCTCTTT CTCATCATCC CCTGCCTGGG GCTGTCTTTC 
CTAACAGTTC TTGTGTTCTA TTTACCTTCG GATGAAGGAG AAAAACTTTC ATTATCCACA 
TCGGTCTTGG TTTCTCTGAC AGTTTTCCTT TTAGTGATTG AAGAAATCAT CCCATCGTCT 
TCCAAAGTCA TTCCTCTCAT TGGAGAGTAC CTGCTGTTCA TCATGATTTT TGTGACCCTG 
TCCATCATTG TTACCGTGTT TGTCATTAAC GTTCACCACA GATCTTCTTC CACGTACCAC 
CCCATGGCCC CCTGGGTTAA GAGGCTCTTT CTGCAGAAAC TTCCAAAATT ACTTTGCATG 
AAAGATCATG TGGATCGCTA CTCATCCCCA GAGAAAGAGG AGAGTCAACC AGTAGTGAAA 
GGCAAAGTCC TCGAAAAAAA GAAACAGAAA CAGCTTAGTG ATGGAGAAAA AGTTCTAGTT 
GCTTTTTTGG AAAAAGCTGC TGATTCCATT AGATACATTT CGAGACATGT GAAGAAAGAA 
CATTTTATCA GCCAGGTAGT ACAAGACTGG AAATTTGTAG CTCAAGTTCT TGACCGAATC 
TTCCTGTGGC TCTTTCTGAT AGTGTCAGTA ACAGGCTCGG TTCTGATTTT TACCCCTGCT 
TTGAAGATGT GGCTACATAG TTACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542692.1
CDS	380..1369
Translation	MTKVIVKSNGTVVWTPPASYKSSCTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLILINENVDRKDFFDNGEWEILNAKGMKGNRRDGVYSYPFITYSFVLRRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSSTYHPMAPWVKRLFLQKLPKLLCMKDHVDRYSSPEKEESQPVVKGKVLEKKKQKQLSDGEKVLVAFLEKAADSIRYISRHVKKEHFISQVVQDWKFVAQVLDRIFLWLFLIVSVTGSVLIFTPALKMWLHSYH

Target ORF information:

RefSeq Version	XM_011544390.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic beta 3 subunit (CHRNB3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544390.2

ORF Insert Sequence:

ATGACCAAGG TCATCGTGAA ATCAAACGGA ACTGTTGTCT GGACCCCTCC CGCCAGCTAC 
AAAAGCTCCT GCACCATGGA CGTCACGTTT TTCCCGTTCG ACCGACAGAA CTGCTCCATG 
AAGTTTGGAT CCTGGACTTA TGATGGCACC ATGGTTGACC TCATTTTGAT CAATGAAAAT 
GTCGACAGAA AAGACTTCTT CGATAACGGA GAATGGGAAA TACTGAACGC AAAGGGGATG 
AAGGGGAACA GAAGGGACGG CGTGTACTCC TATCCCTTTA TCACGTATTC CTTCGTCCTG 
AGACGCCTGC CTTTATTCTA TACCCTCTTT CTCATCATCC CCTGCCTGGG GCTGTCTTTC 
CTAACAGTTC TTGTGTTCTA TTTACCTTCG GATGAAGGAG AAAAACTTTC ATTATCCACA 
TCGGTCTTGG TTTCTCTGAC AGTTTTCCTT TTAGTGATTG AAGAAATCAT CCCATCGTCT 
TCCAAAGTCA TTCCTCTCAT TGGAGAGTAC CTGCTGTTCA TCATGATTTT TGTGACCCTG 
TCCATCATTG TTACCGTGTT TGTCATTAAC GTTCACCACA GATCTTCTTC CACGTACCAC 
CCCATGGCCC CCTGGGTTAA GAGGCTCTTT CTGCAGAAAC TTCCAAAATT ACTTTGCATG 
AAAGATCATG TGGATCGCTA CTCATCCCCA GAGAAAGAGG AGAGTCAACC AGTAGTGAAA 
GGCAAAGTCC TCGAAAAAAA GAAACAGAAA CAGCTTAGTG ATGGAGAAAA AGTTCTAGTT 
GCTTTTTTGG AAAAAGCTGC TGATTCCATT AGATACATTT CGAGACATGT GAAGAAAGAA 
CATTTTATCA GCCAGGTAGT ACAAGACTGG AAATTTGTAG CTCAAGTTCT TGACCGAATC 
TTCCTGTGGC TCTTTCTGAT AGTGTCAGTA ACAGGCTCGG TTCTGATTTT TACCCCTGCT 
TTGAAGATGT GGCTACATAG TTACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Significant association of the CHRNB3-CHRNA6 gene cluster with nicotine dependence in the Chinese Han population.
Scientific reports7(1)9745(2017 Aug)
Wen L,Han H,Liu Q,Su K,Yang Z,Cui W,Yuan W,Ma Y,Fan R,Chen J,Jiang K,Jiang X,Payne TJ,Wang J,Li MD

Unorthodox Acetylcholine Binding Sites Formed by ?5 and ?3 Accessory Subunits in ?4?2* Nicotinic Acetylcholine Receptors.
The Journal of biological chemistry291(45)23452-23463(2016 Nov)
Jain A,Kuryatov A,Wang J,Kamenecka TM,Lindstrom J

Evidence for Association Between Low Frequency Variants in CHRNA6/CHRNB3 and Antisocial Drug Dependence.
Behavior genetics46(5)693-704(2016 Sep)
Kamens HM,Corley RP,Richmond PA,Darlington TM,Dowell R,Hopfer CJ,Stallings MC,Hewitt JK,Brown SA,Ehringer MA

Crucial roles of the CHRNB3-CHRNA6 gene cluster on chromosome 8 in nicotine dependence: update and subjects for future research.
Translational psychiatry6(6)e843(2016 06)
Wen L,Yang Z,Cui W,Li MD

The ?3 subunit of the nicotinic acetylcholine receptor: Modulation of gene expression and nicotine consumption.
Neuropharmacology99639-49(2015 Dec)
Kamens HM,Miyamoto J,Powers MS,Ro K,Soto M,Cox R,Stitzel JA,Ehringer MA

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CHRNB3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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