CHRNA4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRNA4
Entrez Gene ID	1137
Full Name	cholinergic receptor nicotinic alpha 4 subunit
Synonyms	BFNC,EBN,EBN1,NACHR,NACHRA4,NACRA4
General protein information	Preferred Names cholinergic receptor nicotinic alpha 4 subunit Names neuronal acetylcholine receptor subunit alpha-4 cholinergic receptor, nicotinic alpha 4 cholinergic receptor, nicotinic, alpha 4 (neuronal) cholinergic receptor, nicotinic, alpha polypeptide 4 neuronal nicotinic acetylcholine receptor alpha-4 subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q13.33
Summary	This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012].
Disorder MIM:	118504
Disorder Html:	Epilepsy, nocturnal frontal lobe, 1, 600513 (3); {Nicotine addiction, susceptibility to}, 188890 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256573.2	NP_001243502.1	neuronal acetylcholine receptor subunit alpha-4 isoform 2
NM_001256573.1	NP_001243502.1	neuronal acetylcholine receptor subunit alpha-4 isoform 2
XM_017027625.2	XP_016883114.1	neuronal acetylcholine receptor subunit alpha-4 isoform X2
XM_017027624.1	XP_016883113.1	neuronal acetylcholine receptor subunit alpha-4 isoform X2
XM_017027625.1	XP_016883114.1	neuronal acetylcholine receptor subunit alpha-4 isoform X3
NM_000744.6	NP_000735.1	neuronal acetylcholine receptor subunit alpha-4 isoform 1 precursor
XM_011528524.1	XP_011526826.1	neuronal acetylcholine receptor subunit alpha-4 isoform X1
XM_024451822.1	XP_024307590.1	neuronal acetylcholine receptor subunit alpha-4 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CHRNA4	XP_543097.3
Danio rerio (zebrafish)	chrna4b	NP_001041528.1
Xenopus tropicalis (tropical clawed frog)	chrna4	NP_001107315.1
Bos taurus (cattle)	CHRNA4	NP_001179829.1
Danio rerio (zebrafish)	LOC563696	XP_692148.2
Mus musculus (house mouse)	Chrna4	NP_056545.3
Rattus norvegicus (Norway rat)	Chrna4	NP_077330.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000966	XP_309158.3
	CHRNA4	XP_001114265.2
Gallus gallus (chicken)	CHRNA4	NP_990145.1
Homo sapiens (human)	CHRNA4	NP_000735.1
Pan troglodytes (chimpanzee)	CHRNA4	NP_001029286.1
Drosophila melanogaster (fruit fly)	nAcRbeta-64B	NP_523927.2

Gene Ontology
Bibliography

Related articles in PubMed

Overdominant Effect of a
Sadaghiani S, Ng B, Altmann A, Poline JB, Banaschewski T, Bokde ALW, Bromberg U, B?chel C, Burke Quinlan E, Conrod P, Desrivi?res S, Flor H, Frouin V, Garavan H, Gowland P, Gallinat J, Heinz A, Ittermann B, Martinot JL, Paill?re Martinot ML, Lemaitre H, Nees F, Papadopoulos Orfanos D, Paus T, Poustka L, Millenet S, Fr?hner JH, Smolka MN, Walter H, Whelan R, Schumann G, Napolioni V, Greicius M
The Journal of neuroscience : the official journal of the Society for Neuroscience37(40)9657-9666(2017 Oct)

The influence of CHRNA4, COMT, and maternal sensitivity on orienting and executive attention in 6-month-old infants.
Quan J, Ong ML, Bureau JF, Sim LW, Sanmugam S, Abdul Malik AB, Wong E, Wong J, Chong YS, Saw SM, Kwek K, Qiu A, Holbrook JD, Rifkin-Graboi A,
Brain and cognition11617-28(2017 Aug)

The association between the nicotinic acetylcholine receptor ?4 subunit gene (CHRNA4) rs1044396 and Internet gaming disorder in Korean male adults.
Jeong JE, Rhee JK, Kim TM, Kwak SM, Bang SH, Cho H, Cheon YH, Min JA, Yoo GS, Kim K, Choi JS, Choi SW, Kim DJ
PloS one12(12)e0188358(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A polymorphism in CHRNA4 leads to an overdominant effect at this locus, with higher activity in the cingulo-opercular network underlying alertness.
Title: Overdominant Effect of a

Data suggest that amino acid residues alpha4Gly-41, alpha4Lys-64, and alpha4Thr-66 are critical for (alpha4)3(beta2)2 neuronal AChR potentiation by positive allosteric modulator (PAM) CMPI, but not by PAM NS9283; amino acid substitution at alpha4His-116, a known determinant of NS9283 binding and of agonist binding at alpha4:alpha4 subunit interface, did not reduce CMPI potentiation.
Title: Unraveling amino acid residues critical for allosteric potentiation of (?4)3(?2)2-type nicotinic acetylcholine receptor responses.

The form containing three copies of alpha4 and two of beta2 was potentiated at low concentrations of acetylcholine chloride (ACh) and physostigmine, whereas the form containing two copies of alpha4 and three of beta2 was inhibited.
Title: The E Loop of the Transmitter Binding Site Is a Key Determinant of the Modulatory Effects of Physostigmine on Neuronal Nicotinic ?4?2 Receptors.

This study shows that unorthodox acetylcholine binding sites can form at the alpha5/alpha4 and beta3/alpha4 interfaces in (alpha4beta2)2alpha5 and (alpha4beta2)2beta3 nicotinic acetylcholine receptors (nAChRs) and at the alpha4/alpha5 in (beta2alpha4)2alpha5 nAChRs.
Title: Unorthodox Acetylcholine Binding Sites Formed by ?5 and ?3 Accessory Subunits in ?4?2* Nicotinic Acetylcholine Receptors.

X-ray crystallographic structure of the human alpha4beta2 nicotinic receptor, the most abundant nicotinic subtype in the brain; structure provides insights into the architectural principles governing ligand recognition, heteromer assembly, ion permeation and desensitization in this prototypical receptor class
Title: X-ray structure of the human ?4?2 nicotinic receptor.

The following CHRNA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21241	NM_001256573.2 Latest version!	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu21796	NM_000744.6 Latest version!	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu21241	XM_017027625.2 Latest version!	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu21241	XM_024451822.1 Latest version!	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu56880	XM_011528524.1 Latest version!	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu92900	XM_017027624.1 Latest version!	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
View Old Accession Versions >>
OHu21241	NM_001256573.1	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu21241	XM_017027625.1	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRNA4 ( NM_001256573.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243502.1 Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21241
Clone ID Related Accession (Same CDS sequence)	NM_001256573.2 , NM_001256573.1 , XM_017027625.2 , XM_017027625.1 , XM_024451822.1
Accession Version	NM_001256573.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	neuronal acetylcholine receptor subunit alpha-4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62433.1, AL121827.34, BC096292.1, L35901.1, BC096291.1, AK296430.1 and AB209359.1. On Jun 2, 2019 this sequence version replaced NM_001256573.1. Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096292.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2145743, SAMEA2146236 [ECO:0000350] ##Evidence-Data-END##

ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC 
ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC 
TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC 
ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG 
CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC 
CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC 
GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC 
ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC 
CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT 
AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC 
GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT 
GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA 
GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG 
GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA 
GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC 
GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC 
TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG 
CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG 
CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC 
CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG 
GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC 
CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243502.1
CDS	644..1999
Translation	MKFGSWTYDKAKIDLVNMHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVPRLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPPATSGTQSLHPPSPSFCVPLDVPAEPGPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAPGLAKARSLSVQHMSSPGEAVEGGVRCRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPPDQPSPCKCTCKKEPSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI

Target ORF information:

RefSeq Version	NM_001256573.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256573.2

ORF Insert Sequence:

ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC 
ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC 
TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC 
ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG 
CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC 
CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC 
GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC 
ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC 
CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT 
AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC 
GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT 
GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA 
GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG 
GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA 
GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC 
GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC 
TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG 
CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG 
CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC 
CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG 
GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC 
CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNA4 ( NM_001256573.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243502.1 Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21241
Clone ID Related Accession (Same CDS sequence)	NM_001256573.2 , NM_001256573.1 , XM_017027625.2 , XM_017027625.1 , XM_024451822.1
Accession Version	NM_001256573.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	neuronal acetylcholine receptor subunit alpha-4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62433.1, AL121827.34, BC096292.1, L35901.1, BC096291.1, AK296430.1 and AB209359.1. Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096292.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2145743, SAMEA2146236 [ECO:0000350] ##Evidence-Data-END##

ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC 
ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC 
TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC 
ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG 
CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC 
CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC 
GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC 
ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC 
CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT 
AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC 
GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT 
GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA 
GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG 
GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA 
GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC 
GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC 
TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG 
CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG 
CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC 
CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG 
GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC 
CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243502.1
CDS	691..2046
Translation	MKFGSWTYDKAKIDLVNMHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVPRLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPPATSGTQSLHPPSPSFCVPLDVPAEPGPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAPGLAKARSLSVQHMSSPGEAVEGGVRCRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPPDQPSPCKCTCKKEPSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI

Target ORF information:

RefSeq Version	NM_001256573.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256573.1

ORF Insert Sequence:

ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC 
ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC 
TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC 
ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG 
CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC 
CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC 
GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC 
ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC 
CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT 
AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC 
GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT 
GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA 
GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG 
GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA 
GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC 
GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC 
TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG 
CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG 
CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC 
CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG 
GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC 
CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNA4 ( NM_000744.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_000735.1 Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21796
Clone ID Related Accession (Same CDS sequence)	NM_000744.6
Accession Version	NM_000744.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	neuronal acetylcholine receptor subunit alpha-4 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62433.1, AL121827.34, L35901.1, BC096291.1, AK296430.1 and AB209359.1. This sequence is a reference standard in the RefSeqGene project. On Feb 10, 2012 this sequence version replaced NM_000744.5. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U62433.1, BC096290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein regulatory uORF :: PMID: 23843950 ##RefSeq-Attributes-END##

ATGGAGCTAG GGGGCCCCGG AGCGCCGCGG CTGCTGCCGC CGCTGCTGCT GCTTCTGGGG 
ACCGGCCTCC TGCGCGCCAG CAGCCATGTG GAGACCCGGG CCCACGCCGA GGAGCGGCTC 
CTGAAGAAAC TCTTCTCCGG TTACAACAAG TGGTCCCGAC CCGTGGCCAA CATCTCGGAC 
GTGGTCCTCG TCCGCTTCGG CCTGTCCATC GCTCAGCTCA TTGACGTGGA TGAGAAGAAC 
CAGATGATGA CCACGAACGT ATGGGTGAAG CAGGAGTGGC ACGACTACAA GCTGCGCTGG 
GACCCAGCTG ACTATGAGAA TGTCACCTCC ATCCGCATCC CCTCCGAGCT CATCTGGCGG 
CCGGACATCG TCCTCTACAA CAATGCTGAC GGGGACTTCG CGGTCACCCA CCTGACCAAG 
GCCCACCTGT TCCATGACGG GCGGGTGCAG TGGACTCCCC CGGCCATTTA CAAGAGCTCC 
TGCAGCATCG ACGTCACCTT CTTCCCCTTC GACCAGCAGA ACTGCACCAT GAAATTCGGC 
TCCTGGACCT ACGACAAGGC CAAGATCGAC CTGGTGAACA TGCACAGCCG CGTGGACCAG 
CTGGACTTCT GGGAGAGTGG CGAGTGGGTC ATCGTGGATG CCGTGGGCAC CTACAACACC 
AGGAAGTACG AGTGCTGTGC CGAGATCTAC CCGGACATCA CCTATGCCTT CGTCATCCGG 
CGGCTGCCGC TCTTCTACAC CATCAACCTC ATCATCCCCT GCCTGCTCAT CTCCTGCCTC 
ACCGTGCTGG TCTTCTACCT GCCCTCCGAG TGTGGCGAGA AGATCACGCT GTGCATCTCC 
GTGCTGCTGT CGCTCACCGT CTTCCTGCTG CTCATCACCG AGATCATCCC GTCCACCTCA 
CTGGTCATCC CACTCATCGG CGAGTACCTG CTGTTCACCA TGATCTTCGT CACCCTGTCC 
ATCGTCATCA CGGTCTTCGT GCTCAACGTG CACCACCGCT CGCCACGCAC GCACACCATG 
CCCACCTGGG TACGCAGGGT CTTCCTGGAC ATCGTGCCAC GCCTGCTCCT CATGAAGCGG 
CCGTCCGTGG TCAAGGACAA TTGCCGGCGG CTCATCGAGT CCATGCATAA GATGGCCAGT 
GCCCCGCGCT TCTGGCCCGA GCCAGAAGGG GAGCCCCCTG CCACGAGCGG CACCCAGAGC 
CTGCACCCGC CCTCACCGTC CTTCTGTGTC CCCCTGGATG TGCCGGCTGA GCCTGGGCCT 
TCCTGCAAGT CACCCTCCGA CCAGCTCCCT CCTCAGCAGC CCCTGGAAGC TGAGAAAGCC 
AGCCCCCACC CCTCGCCTGG ACCCTGCCGC CCGCCCCACG GCACCCAGGC ACCAGGGCTG 
GCCAAAGCCA GGTCCCTCAG CGTCCAGCAC ATGTCCAGCC CTGGCGAAGC GGTGGAAGGC 
GGCGTCCGGT GCCGGTCTCG GAGCATCCAG TACTGTGTTC CCCGAGACGA TGCCGCCCCC 
GAGGCAGATG GCCAGGCTGC CGGCGCCCTG GCCTCTCGCA ACACCCACTC GGCTGAGCTC 
CCACCCCCAG ACCAGCCCTC TCCGTGCAAA TGCACATGCA AGAAGGAGCC CTCTTCGGTG 
TCCCCGAGCG CCACGGTCAA GACCCGCAGC ACCAAAGCGC CGCCCCCGCA CCTGCCCCTG 
TCGCCGGCCC TGACCCGGGC GGTGGAGGGC GTCCAGTACA TTGCAGACCA CCTGAAGGCC 
GAAGACACAG ACTTCTCGGT GAAGGAGGAC TGGAAGTACG TGGCCATGGT CATCGACCGC 
ATCTTCCTCT GGATGTTCAT CATCGTCTGC CTGCTGGGGA CGGTGGGCCT CTTCCTGCCG 
CCCTGGCTGG CTGGCATGAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000735.1
CDS	232..2115
Translation	MELGGPGAPRLLPPLLLLLGTGLLRASSHVETRAHAEERLLKKLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPADYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFHDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVNMHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVPRLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPPATSGTQSLHPPSPSFCVPLDVPAEPGPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAPGLAKARSLSVQHMSSPGEAVEGGVRCRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPPDQPSPCKCTCKKEPSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI

Target ORF information:

RefSeq Version	NM_000744.6
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000744.6

ORF Insert Sequence:

ATGGAGCTAG GGGGCCCCGG AGCGCCGCGG CTGCTGCCGC CGCTGCTGCT GCTTCTGGGG 
ACCGGCCTCC TGCGCGCCAG CAGCCATGTG GAGACCCGGG CCCACGCCGA GGAGCGGCTC 
CTGAAGAAAC TCTTCTCCGG TTACAACAAG TGGTCCCGAC CCGTGGCCAA CATCTCGGAC 
GTGGTCCTCG TCCGCTTCGG CCTGTCCATC GCTCAGCTCA TTGACGTGGA TGAGAAGAAC 
CAGATGATGA CCACGAACGT ATGGGTGAAG CAGGAGTGGC ACGACTACAA GCTGCGCTGG 
GACCCAGCTG ACTATGAGAA TGTCACCTCC ATCCGCATCC CCTCCGAGCT CATCTGGCGG 
CCGGACATCG TCCTCTACAA CAATGCTGAC GGGGACTTCG CGGTCACCCA CCTGACCAAG 
GCCCACCTGT TCCATGACGG GCGGGTGCAG TGGACTCCCC CGGCCATTTA CAAGAGCTCC 
TGCAGCATCG ACGTCACCTT CTTCCCCTTC GACCAGCAGA ACTGCACCAT GAAATTCGGC 
TCCTGGACCT ACGACAAGGC CAAGATCGAC CTGGTGAACA TGCACAGCCG CGTGGACCAG 
CTGGACTTCT GGGAGAGTGG CGAGTGGGTC ATCGTGGATG CCGTGGGCAC CTACAACACC 
AGGAAGTACG AGTGCTGTGC CGAGATCTAC CCGGACATCA CCTATGCCTT CGTCATCCGG 
CGGCTGCCGC TCTTCTACAC CATCAACCTC ATCATCCCCT GCCTGCTCAT CTCCTGCCTC 
ACCGTGCTGG TCTTCTACCT GCCCTCCGAG TGTGGCGAGA AGATCACGCT GTGCATCTCC 
GTGCTGCTGT CGCTCACCGT CTTCCTGCTG CTCATCACCG AGATCATCCC GTCCACCTCA 
CTGGTCATCC CACTCATCGG CGAGTACCTG CTGTTCACCA TGATCTTCGT CACCCTGTCC 
ATCGTCATCA CGGTCTTCGT GCTCAACGTG CACCACCGCT CGCCACGCAC GCACACCATG 
CCCACCTGGG TACGCAGGGT CTTCCTGGAC ATCGTGCCAC GCCTGCTCCT CATGAAGCGG 
CCGTCCGTGG TCAAGGACAA TTGCCGGCGG CTCATCGAGT CCATGCATAA GATGGCCAGT 
GCCCCGCGCT TCTGGCCCGA GCCAGAAGGG GAGCCCCCTG CCACGAGCGG CACCCAGAGC 
CTGCACCCGC CCTCACCGTC CTTCTGTGTC CCCCTGGATG TGCCGGCTGA GCCTGGGCCT 
TCCTGCAAGT CACCCTCCGA CCAGCTCCCT CCTCAGCAGC CCCTGGAAGC TGAGAAAGCC 
AGCCCCCACC CCTCGCCTGG ACCCTGCCGC CCGCCCCACG GCACCCAGGC ACCAGGGCTG 
GCCAAAGCCA GGTCCCTCAG CGTCCAGCAC ATGTCCAGCC CTGGCGAAGC GGTGGAAGGC 
GGCGTCCGGT GCCGGTCTCG GAGCATCCAG TACTGTGTTC CCCGAGACGA TGCCGCCCCC 
GAGGCAGATG GCCAGGCTGC CGGCGCCCTG GCCTCTCGCA ACACCCACTC GGCTGAGCTC 
CCACCCCCAG ACCAGCCCTC TCCGTGCAAA TGCACATGCA AGAAGGAGCC CTCTTCGGTG 
TCCCCGAGCG CCACGGTCAA GACCCGCAGC ACCAAAGCGC CGCCCCCGCA CCTGCCCCTG 
TCGCCGGCCC TGACCCGGGC GGTGGAGGGC GTCCAGTACA TTGCAGACCA CCTGAAGGCC 
GAAGACACAG ACTTCTCGGT GAAGGAGGAC TGGAAGTACG TGGCCATGGT CATCGACCGC 
ATCTTCCTCT GGATGTTCAT CATCGTCTGC CTGCTGGGGA CGGTGGGCCT CTTCCTGCCG 
CCCTGGCTGG CTGGCATGAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNA4 ( XM_017027625.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883114.1 Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X2, mRNA.

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CloneID	OHu21241
Clone ID Related Accession (Same CDS sequence)	NM_001256573.2 , NM_001256573.1 , XM_017027625.2 , XM_017027625.1 , XM_024451822.1
Accession Version	XM_017027625.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neuronal acetylcholine receptor subunit alpha-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017027625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC 
ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC 
TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC 
ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG 
CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC 
CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC 
GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC 
ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC 
CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT 
AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC 
GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT 
GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA 
GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG 
GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA 
GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC 
GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC 
TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG 
CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG 
CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC 
CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG 
GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC 
CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883114.1
CDS	889..2244
Translation	MKFGSWTYDKAKIDLVNMHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVPRLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPPATSGTQSLHPPSPSFCVPLDVPAEPGPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAPGLAKARSLSVQHMSSPGEAVEGGVRCRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPPDQPSPCKCTCKKEPSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI

Target ORF information:

RefSeq Version	XM_017027625.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027625.2

ORF Insert Sequence:

ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC 
ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC 
TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC 
ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG 
CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC 
CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC 
GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC 
ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC 
CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT 
AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC 
GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT 
GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA 
GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG 
GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA 
GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC 
GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC 
TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG 
CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG 
CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC 
CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG 
GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC 
CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNA4 ( XM_017027625.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883114.1 Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X3, mRNA.

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CloneID	OHu21241
Clone ID Related Accession (Same CDS sequence)	NM_001256573.2 , NM_001256573.1 , XM_017027625.2 , XM_017027625.1 , XM_024451822.1
Accession Version	XM_017027625.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neuronal acetylcholine receptor subunit alpha-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC 
ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC 
TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC 
ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG 
CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC 
CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC 
GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC 
ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC 
CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT 
AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC 
GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT 
GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA 
GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG 
GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA 
GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC 
GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC 
TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG 
CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG 
CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC 
CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG 
GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC 
CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883114.1
CDS	810..2165
Translation	MKFGSWTYDKAKIDLVNMHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVPRLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPPATSGTQSLHPPSPSFCVPLDVPAEPGPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAPGLAKARSLSVQHMSSPGEAVEGGVRCRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPPDQPSPCKCTCKKEPSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI

Target ORF information:

RefSeq Version	XM_017027625.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027625.1

ORF Insert Sequence:

ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC 
ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC 
TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC 
ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG 
CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC 
CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC 
GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC 
ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC 
CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT 
AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC 
GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT 
GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA 
GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG 
GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA 
GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC 
GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC 
TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG 
CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG 
CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC 
CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG 
GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC 
CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNA4 ( XM_024451822.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307590.1 Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu21241
Clone ID Related Accession (Same CDS sequence)	NM_001256573.2 , NM_001256573.1 , XM_017027625.2 , XM_017027625.1 , XM_024451822.1
Accession Version	XM_024451822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neuronal acetylcholine receptor subunit alpha-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC 
ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC 
TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC 
ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG 
CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC 
CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC 
GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC 
ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC 
CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT 
AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC 
GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT 
GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA 
GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG 
GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA 
GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC 
GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC 
TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG 
CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG 
CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC 
CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG 
GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC 
CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307590.1
CDS	234..1589
Translation	MKFGSWTYDKAKIDLVNMHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVPRLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPPATSGTQSLHPPSPSFCVPLDVPAEPGPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAPGLAKARSLSVQHMSSPGEAVEGGVRCRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPPDQPSPCKCTCKKEPSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI

Target ORF information:

RefSeq Version	XM_024451822.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451822.1

ORF Insert Sequence:

ATGAAATTCG GCTCCTGGAC CTACGACAAG GCCAAGATCG ACCTGGTGAA CATGCACAGC 
CGCGTGGACC AGCTGGACTT CTGGGAGAGT GGCGAGTGGG TCATCGTGGA TGCCGTGGGC 
ACCTACAACA CCAGGAAGTA CGAGTGCTGT GCCGAGATCT ACCCGGACAT CACCTATGCC 
TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC 
ATCTCCTGCC TCACCGTGCT GGTCTTCTAC CTGCCCTCCG AGTGTGGCGA GAAGATCACG 
CTGTGCATCT CCGTGCTGCT GTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC 
CCGTCCACCT CACTGGTCAT CCCACTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC 
GTCACCCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCACGC 
ACGCACACCA TGCCCACCTG GGTACGCAGG GTCTTCCTGG ACATCGTGCC ACGCCTGCTC 
CTCATGAAGC GGCCGTCCGT GGTCAAGGAC AATTGCCGGC GGCTCATCGA GTCCATGCAT 
AAGATGGCCA GTGCCCCGCG CTTCTGGCCC GAGCCAGAAG GGGAGCCCCC TGCCACGAGC 
GGCACCCAGA GCCTGCACCC GCCCTCACCG TCCTTCTGTG TCCCCCTGGA TGTGCCGGCT 
GAGCCTGGGC CTTCCTGCAA GTCACCCTCC GACCAGCTCC CTCCTCAGCA GCCCCTGGAA 
GCTGAGAAAG CCAGCCCCCA CCCCTCGCCT GGACCCTGCC GCCCGCCCCA CGGCACCCAG 
GCACCAGGGC TGGCCAAAGC CAGGTCCCTC AGCGTCCAGC ACATGTCCAG CCCTGGCGAA 
GCGGTGGAAG GCGGCGTCCG GTGCCGGTCT CGGAGCATCC AGTACTGTGT TCCCCGAGAC 
GATGCCGCCC CCGAGGCAGA TGGCCAGGCT GCCGGCGCCC TGGCCTCTCG CAACACCCAC 
TCGGCTGAGC TCCCACCCCC AGACCAGCCC TCTCCGTGCA AATGCACATG CAAGAAGGAG 
CCCTCTTCGG TGTCCCCGAG CGCCACGGTC AAGACCCGCA GCACCAAAGC GCCGCCCCCG 
CACCTGCCCC TGTCGCCGGC CCTGACCCGG GCGGTGGAGG GCGTCCAGTA CATTGCAGAC 
CACCTGAAGG CCGAAGACAC AGACTTCTCG GTGAAGGAGG ACTGGAAGTA CGTGGCCATG 
GTCATCGACC GCATCTTCCT CTGGATGTTC ATCATCGTCT GCCTGCTGGG GACGGTGGGC 
CTCTTCCTGC CGCCCTGGCT GGCTGGCATG ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNA4 ( XM_011528524.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526826.1 Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X1, mRNA.

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CloneID	OHu56880
Clone ID Related Accession (Same CDS sequence)	XM_011528524.1
Accession Version	XM_011528524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neuronal acetylcholine receptor subunit alpha-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAATGT CACCTCCATC CGCATCCCCT CCGAGCTCAT CTGGCGGCCG GACATCGTCC 
TCTACAACAA CTGCAGGAGA GCCCTTTTGG GCAGGAGTGC TTTTCGCCAT CAGACCCCAT 
CCTGGCCTTT CTGGGAGAAT AGTGTGGACT GCAGGGTGCC CTGGAGAGGG TGCTGACGGG 
GACTTCGCGG TCACCCACCT GACCAAGGCC CACCTGTTCC ATGACGGGCG GGTGCAGTGG 
ACTCCCCCGG CCATTTACAA GAGCTCCTGC AGCATCGACG TCACCTTCTT CCCCTTCGAC 
CAGCAGAACT GCACCATGAA ATTCGGCTCC TGGACCTACG ACAAGGCCAA GATCGACCTG 
GTGAACATGC ACAGCCGCGT GGACCAGCTG GACTTCTGGG AGAGTGGCGA GTGGGTCATC 
GTGGATGCCG TGGGCACCTA CAACACCAGG AAGTACGAGT GCTGTGCCGA GATCTACCCG 
GACATCACCT ATGCCTTCGT CATCCGGCGG CTGCCGCTCT TCTACACCAT CAACCTCATC 
ATCCCCTGCC TGCTCATCTC CTGCCTCACC GTGCTGGTCT TCTACCTGCC CTCCGAGTGT 
GGCGAGAAGA TCACGCTGTG CATCTCCGTG CTGCTGTCGC TCACCGTCTT CCTGCTGCTC 
ATCACCGAGA TCATCCCGTC CACCTCACTG GTCATCCCAC TCATCGGCGA GTACCTGCTG 
TTCACCATGA TCTTCGTCAC CCTGTCCATC GTCATCACGG TCTTCGTGCT CAACGTGCAC 
CACCGCTCGC CACGCACGCA CACCATGCCC ACCTGGGTAC GCAGGGTCTT CCTGGACATC 
GTGCCACGCC TGCTCCTCAT GAAGCGGCCG TCCGTGGTCA AGGACAATTG CCGGCGGCTC 
ATCGAGTCCA TGCATAAGAT GGCCAGTGCC CCGCGCTTCT GGCCCGAGCC AGAAGGGGAG 
CCCCCTGCCA CGAGCGGCAC CCAGAGCCTG CACCCGCCCT CACCGTCCTT CTGTGTCCCC 
CTGGATGTGC CGGCTGAGCC TGGGCCTTCC TGCAAGTCAC CCTCCGACCA GCTCCCTCCT 
CAGCAGCCCC TGGAAGCTGA GAAAGCCAGC CCCCACCCCT CGCCTGGACC CTGCCGCCCG 
CCCCACGGCA CCCAGGCACC AGGGCTGGCC AAAGCCAGGT CCCTCAGCGT CCAGCACATG 
TCCAGCCCTG GCGAAGCGGT GGAAGGCGGC GTCCGGTGCC GGTCTCGGAG CATCCAGTAC 
TGTGTTCCCC GAGACGATGC CGCCCCCGAG GCAGATGGCC AGGCTGCCGG CGCCCTGGCC 
TCTCGCAACA CCCACTCGGC TGAGCTCCCA CCCCCAGACC AGCCCTCTCC GTGCAAATGC 
ACATGCAAGA AGGAGCCCTC TTCGGTGTCC CCGAGCGCCA CGGTCAAGAC CCGCAGCACC 
AAAGCGCCGC CCCCGCACCT GCCCCTGTCG CCGGCCCTGA CCCGGGCGGT GGAGGGCGTC 
CAGTACATTG CAGACCACCT GAAGGCCGAA GACACAGACT TCTCGGTGAA GGAGGACTGG 
AAGTACGTGG CCATGGTCAT CGACCGCATC TTCCTCTGGA TGTTCATCAT CGTCTGCCTG 
CTGGGGACGG TGGGCCTCTT CCTGCCGCCC TGGCTGGCTG GCATGATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526826.1
CDS	89..1759
Translation	MRMSPPSASPPSSSGGRTSSSTTTAGEPFWAGVLFAIRPHPGLSGRIVWTAGCPGEGADGDFAVTHLTKAHLFHDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVNMHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVPRLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPPATSGTQSLHPPSPSFCVPLDVPAEPGPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAPGLAKARSLSVQHMSSPGEAVEGGVRCRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPPDQPSPCKCTCKKEPSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI

Target ORF information:

RefSeq Version	XM_011528524.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528524.1

ORF Insert Sequence:

ATGAGAATGT CACCTCCATC CGCATCCCCT CCGAGCTCAT CTGGCGGCCG GACATCGTCC 
TCTACAACAA CTGCAGGAGA GCCCTTTTGG GCAGGAGTGC TTTTCGCCAT CAGACCCCAT 
CCTGGCCTTT CTGGGAGAAT AGTGTGGACT GCAGGGTGCC CTGGAGAGGG TGCTGACGGG 
GACTTCGCGG TCACCCACCT GACCAAGGCC CACCTGTTCC ATGACGGGCG GGTGCAGTGG 
ACTCCCCCGG CCATTTACAA GAGCTCCTGC AGCATCGACG TCACCTTCTT CCCCTTCGAC 
CAGCAGAACT GCACCATGAA ATTCGGCTCC TGGACCTACG ACAAGGCCAA GATCGACCTG 
GTGAACATGC ACAGCCGCGT GGACCAGCTG GACTTCTGGG AGAGTGGCGA GTGGGTCATC 
GTGGATGCCG TGGGCACCTA CAACACCAGG AAGTACGAGT GCTGTGCCGA GATCTACCCG 
GACATCACCT ATGCCTTCGT CATCCGGCGG CTGCCGCTCT TCTACACCAT CAACCTCATC 
ATCCCCTGCC TGCTCATCTC CTGCCTCACC GTGCTGGTCT TCTACCTGCC CTCCGAGTGT 
GGCGAGAAGA TCACGCTGTG CATCTCCGTG CTGCTGTCGC TCACCGTCTT CCTGCTGCTC 
ATCACCGAGA TCATCCCGTC CACCTCACTG GTCATCCCAC TCATCGGCGA GTACCTGCTG 
TTCACCATGA TCTTCGTCAC CCTGTCCATC GTCATCACGG TCTTCGTGCT CAACGTGCAC 
CACCGCTCGC CACGCACGCA CACCATGCCC ACCTGGGTAC GCAGGGTCTT CCTGGACATC 
GTGCCACGCC TGCTCCTCAT GAAGCGGCCG TCCGTGGTCA AGGACAATTG CCGGCGGCTC 
ATCGAGTCCA TGCATAAGAT GGCCAGTGCC CCGCGCTTCT GGCCCGAGCC AGAAGGGGAG 
CCCCCTGCCA CGAGCGGCAC CCAGAGCCTG CACCCGCCCT CACCGTCCTT CTGTGTCCCC 
CTGGATGTGC CGGCTGAGCC TGGGCCTTCC TGCAAGTCAC CCTCCGACCA GCTCCCTCCT 
CAGCAGCCCC TGGAAGCTGA GAAAGCCAGC CCCCACCCCT CGCCTGGACC CTGCCGCCCG 
CCCCACGGCA CCCAGGCACC AGGGCTGGCC AAAGCCAGGT CCCTCAGCGT CCAGCACATG 
TCCAGCCCTG GCGAAGCGGT GGAAGGCGGC GTCCGGTGCC GGTCTCGGAG CATCCAGTAC 
TGTGTTCCCC GAGACGATGC CGCCCCCGAG GCAGATGGCC AGGCTGCCGG CGCCCTGGCC 
TCTCGCAACA CCCACTCGGC TGAGCTCCCA CCCCCAGACC AGCCCTCTCC GTGCAAATGC 
ACATGCAAGA AGGAGCCCTC TTCGGTGTCC CCGAGCGCCA CGGTCAAGAC CCGCAGCACC 
AAAGCGCCGC CCCCGCACCT GCCCCTGTCG CCGGCCCTGA CCCGGGCGGT GGAGGGCGTC 
CAGTACATTG CAGACCACCT GAAGGCCGAA GACACAGACT TCTCGGTGAA GGAGGACTGG 
AAGTACGTGG CCATGGTCAT CGACCGCATC TTCCTCTGGA TGTTCATCAT CGTCTGCCTG 
CTGGGGACGG TGGGCCTCTT CCTGCCGCCC TGGCTGGCTG GCATGATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNA4 ( XM_017027624.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883113.1 Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu92900
Clone ID Related Accession (Same CDS sequence)	XM_017027624.1
Accession Version	XM_017027624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neuronal acetylcholine receptor subunit alpha-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACCA CGAACGTATG GGTGAAGCAG GAGTGGCACG ACTACAAGCT GCGCTGGGAC 
CCAGCTGACT ATGAGAATGT CACCTCCATC CGCATCCCCT CCGAGCTCAT CTGGCGGCCG 
GACATCGTCC TCTACAACAA TGCTGACGGG GACTTCGCGG TCACCCACCT GACCAAGGCC 
CACCTGTTCC ATGACGGGCG GGTGCAGTGG ACTCCCCCGG CCATTTACAA GAGCTCCTGC 
AGCATCGACG TCACCTTCTT CCCCTTCGAC CAGCAGAACT GCACCATGAA ATTCGGCTCC 
TGGACCTACG ACAAGGCCAA GATCGACCTG GTGAACATGC ACAGCCGCGT GGACCAGCTG 
GACTTCTGGG AGAGTGGCGA GTGGGTCATC GTGGATGCCG TGGGCACCTA CAACACCAGG 
AAGTACGAGT GCTGTGCCGA GATCTACCCG GACATCACCT ATGCCTTCGT CATCCGGCGG 
CTGCCGCTCT TCTACACCAT CAACCTCATC ATCCCCTGCC TGCTCATCTC CTGCCTCACC 
GTGCTGGTCT TCTACCTGCC CTCCGAGTGT GGCGAGAAGA TCACGCTGTG CATCTCCGTG 
CTGCTGTCGC TCACCGTCTT CCTGCTGCTC ATCACCGAGA TCATCCCGTC CACCTCACTG 
GTCATCCCAC TCATCGGCGA GTACCTGCTG TTCACCATGA TCTTCGTCAC CCTGTCCATC 
GTCATCACGG TCTTCGTGCT CAACGTGCAC CACCGCTCGC CACGCACGCA CACCATGCCC 
ACCTGGGTAC GCAGGGTCTT CCTGGACATC GTGCCACGCC TGCTCCTCAT GAAGCGGCCG 
TCCGTGGTCA AGGACAATTG CCGGCGGCTC ATCGAGTCCA TGCATAAGAT GGCCAGTGCC 
CCGCGCTTCT GGCCCGAGCC AGAAGGGGAG CCCCCTGCCA CGAGCGGCAC CCAGAGCCTG 
CACCCGCCCT CACCGTCCTT CTGTGTCCCC CTGGATGTGC CGGCTGAGCC TGGGCCTTCC 
TGCAAGTCAC CCTCCGACCA GCTCCCTCCT CAGCAGCCCC TGGAAGCTGA GAAAGCCAGC 
CCCCACCCCT CGCCTGGACC CTGCCGCCCG CCCCACGGCA CCCAGGCACC AGGGCTGGCC 
AAAGCCAGGT CCCTCAGCGT CCAGCACATG TCCAGCCCTG GCGAAGCGGT GGAAGGCGGC 
GTCCGGTGCC GGTCTCGGAG CATCCAGTAC TGTGTTCCCC GAGACGATGC CGCCCCCGAG 
GCAGATGGCC AGGCTGCCGG CGCCCTGGCC TCTCGCAACA CCCACTCGGC TGAGCTCCCA 
CCCCCAGACC AGCCCTCTCC GTGCAAATGC ACATGCAAGA AGGAGCCCTC TTCGGTGTCC 
CCGAGCGCCA CGGTCAAGAC CCGCAGCACC AAAGCGCCGC CCCCGCACCT GCCCCTGTCG 
CCGGCCCTGA CCCGGGCGGT GGAGGGCGTC CAGTACATTG CAGACCACCT GAAGGCCGAA 
GACACAGACT TCTCGGTGAA GGAGGACTGG AAGTACGTGG CCATGGTCAT CGACCGCATC 
TTCCTCTGGA TGTTCATCAT CGTCTGCCTG CTGGGGACGG TGGGCCTCTT CCTGCCGCCC 
TGGCTGGCTG GCATGATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883113.1
CDS	507..2147
Translation	MMTTNVWVKQEWHDYKLRWDPADYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFHDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVNMHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVPRLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPPATSGTQSLHPPSPSFCVPLDVPAEPGPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAPGLAKARSLSVQHMSSPGEAVEGGVRCRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPPDQPSPCKCTCKKEPSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI

Target ORF information:

RefSeq Version	XM_017027624.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027624.1

ORF Insert Sequence:

ATGATGACCA CGAACGTATG GGTGAAGCAG GAGTGGCACG ACTACAAGCT GCGCTGGGAC 
CCAGCTGACT ATGAGAATGT CACCTCCATC CGCATCCCCT CCGAGCTCAT CTGGCGGCCG 
GACATCGTCC TCTACAACAA TGCTGACGGG GACTTCGCGG TCACCCACCT GACCAAGGCC 
CACCTGTTCC ATGACGGGCG GGTGCAGTGG ACTCCCCCGG CCATTTACAA GAGCTCCTGC 
AGCATCGACG TCACCTTCTT CCCCTTCGAC CAGCAGAACT GCACCATGAA ATTCGGCTCC 
TGGACCTACG ACAAGGCCAA GATCGACCTG GTGAACATGC ACAGCCGCGT GGACCAGCTG 
GACTTCTGGG AGAGTGGCGA GTGGGTCATC GTGGATGCCG TGGGCACCTA CAACACCAGG 
AAGTACGAGT GCTGTGCCGA GATCTACCCG GACATCACCT ATGCCTTCGT CATCCGGCGG 
CTGCCGCTCT TCTACACCAT CAACCTCATC ATCCCCTGCC TGCTCATCTC CTGCCTCACC 
GTGCTGGTCT TCTACCTGCC CTCCGAGTGT GGCGAGAAGA TCACGCTGTG CATCTCCGTG 
CTGCTGTCGC TCACCGTCTT CCTGCTGCTC ATCACCGAGA TCATCCCGTC CACCTCACTG 
GTCATCCCAC TCATCGGCGA GTACCTGCTG TTCACCATGA TCTTCGTCAC CCTGTCCATC 
GTCATCACGG TCTTCGTGCT CAACGTGCAC CACCGCTCGC CACGCACGCA CACCATGCCC 
ACCTGGGTAC GCAGGGTCTT CCTGGACATC GTGCCACGCC TGCTCCTCAT GAAGCGGCCG 
TCCGTGGTCA AGGACAATTG CCGGCGGCTC ATCGAGTCCA TGCATAAGAT GGCCAGTGCC 
CCGCGCTTCT GGCCCGAGCC AGAAGGGGAG CCCCCTGCCA CGAGCGGCAC CCAGAGCCTG 
CACCCGCCCT CACCGTCCTT CTGTGTCCCC CTGGATGTGC CGGCTGAGCC TGGGCCTTCC 
TGCAAGTCAC CCTCCGACCA GCTCCCTCCT CAGCAGCCCC TGGAAGCTGA GAAAGCCAGC 
CCCCACCCCT CGCCTGGACC CTGCCGCCCG CCCCACGGCA CCCAGGCACC AGGGCTGGCC 
AAAGCCAGGT CCCTCAGCGT CCAGCACATG TCCAGCCCTG GCGAAGCGGT GGAAGGCGGC 
GTCCGGTGCC GGTCTCGGAG CATCCAGTAC TGTGTTCCCC GAGACGATGC CGCCCCCGAG 
GCAGATGGCC AGGCTGCCGG CGCCCTGGCC TCTCGCAACA CCCACTCGGC TGAGCTCCCA 
CCCCCAGACC AGCCCTCTCC GTGCAAATGC ACATGCAAGA AGGAGCCCTC TTCGGTGTCC 
CCGAGCGCCA CGGTCAAGAC CCGCAGCACC AAAGCGCCGC CCCCGCACCT GCCCCTGTCG 
CCGGCCCTGA CCCGGGCGGT GGAGGGCGTC CAGTACATTG CAGACCACCT GAAGGCCGAA 
GACACAGACT TCTCGGTGAA GGAGGACTGG AAGTACGTGG CCATGGTCAT CGACCGCATC 
TTCCTCTGGA TGTTCATCAT CGTCTGCCTG CTGGGGACGG TGGGCCTCTT CCTGCCGCCC 
TGGCTGGCTG GCATGATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overdominant Effect of a
The Journal of neuroscience : the official journal of the Society for Neuroscience37(40)9657-9666(2017 Oct)
Sadaghiani S,Ng B,Altmann A,Poline JB,Banaschewski T,Bokde ALW,Bromberg U,B?chel C,Burke Quinlan E,Conrod P,Desrivi?res S,Flor H,Frouin V,Garavan H,Gowland P,Gallinat J,Heinz A,Ittermann B,Martinot JL,Paill?re Martinot ML,Lemaitre H,Nees F,Papadopoulos Orfanos D,Paus T,Poustka L,Millenet S,Fr?hner JH,Smolka MN,Walter H,Whelan R,Schumann G,Napolioni V,Greicius M

The influence of CHRNA4, COMT, and maternal sensitivity on orienting and executive attention in 6-month-old infants.
Brain and cognition11617-28(2017 Aug)
Quan J,Ong ML,Bureau JF,Sim LW,Sanmugam S,Abdul Malik AB,Wong E,Wong J,Chong YS,Saw SM,Kwek K,Qiu A,Holbrook JD,Rifkin-Graboi A,

The association between the nicotinic acetylcholine receptor ?4 subunit gene (CHRNA4) rs1044396 and Internet gaming disorder in Korean male adults.
PloS one12(12)e0188358(2017)
Jeong JE,Rhee JK,Kim TM,Kwak SM,Bang SH,Cho H,Cheon YH,Min JA,Yoo GS,Kim K,Choi JS,Choi SW,Kim DJ

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CHRNA4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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