CHRNA1 cDNA ORF clone, Homo sapiens(human)

The following CHRNA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu19640 NM_001039523.3
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$204.50-$286.30
$409.00
OHu16431 NM_000079.4
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu88974 XM_017003256.1
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu88975 XM_017003257.1
Latest version!
Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OHu19640 NM_001039523.2 Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$204.50-$286.30
$409.00
OHu16431 NM_000079.3 Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $272.30
$389.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu19640
    Clone ID Related Accession (Same CDS sequence) NM_001039523.3 , NM_001039523.2
    Accession Version NM_001039523.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product acetylcholine receptor subunit alpha isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315312.1, CD013889.1 and BC043196.2. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001039523.2. Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.102475.1, AK299445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162946, SAMN03267754 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
    TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG
    CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC
    ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGGGTGAC
    ATGGTAGATC TGCCACGCCC CAGCTGCGTG ACTTTGGGAG TTCCTTTGTT TTCTCATCTG
    CAGAATGAGC AATGGGTGGA TTACAACCTA AAATGGAATC CAGATGACTA TGGCGGTGTG
    AAAAAAATTC ACATTCCTTC AGAAAAGATC TGGCGCCCAG ACCTTGTTCT CTATAACAAT
    GCAGATGGTG ACTTTGCTAT TGTCAAGTTC ACCAAAGTGC TCCTGCAGTA CACTGGCCAC
    ATCACGTGGA CACCTCCAGC CATCTTTAAA AGCTACTGTG AGATCATCGT CACCCACTTT
    CCCTTTGATG AACAGAACTG CAGCATGAAG CTGGGCACCT GGACCTACGA CGGCTCTGTC
    GTGGCCATCA ACCCGGAAAG CGACCAGCCA GACCTGAGCA ACTTCATGGA GAGCGGGGAG
    TGGGTGATCA AGGAGTCCCG GGGCTGGAAG CACTCCGTGA CCTATTCCTG CTGCCCCGAC
    ACCCCCTACC TGGACATCAC CTACCACTTC GTCATGCAGC GCCTGCCCCT CTACTTCATC
    GTCAACGTCA TCATCCCCTG CCTGCTCTTC TCCTTCTTAA CTGGCCTGGT ATTCTACCTG
    CCCACAGACT CAGGGGAGAA GATGACTCTG AGCATCTCTG TCTTACTGTC TTTGACTGTG
    TTCCTTCTGG TCATCGTGGA GCTGATCCCC TCCACGTCCA GTGCTGTGCC CTTGATTGGA
    AAATACATGC TGTTCACCAT GGTGTTCGTC ATTGCCTCCA TCATCATCAC TGTCATCGTC
    ATCAACACAC ACCACCGCTC ACCCAGCACC CATGTCATGC CCAACTGGGT GCGGAAGGTT
    TTTATCGACA CTATCCCAAA TATCATGTTT TTCTCCACAA TGAAAAGACC ATCCAGAGAA
    AAGCAAGACA AAAAGATTTT TACAGAAGAC ATTGATATCT CTGACATTTC TGGAAAGCCA
    GGGCCTCCAC CCATGGGCTT CCACTCTCCC CTGATCAAAC ACCCCGAGGT GAAAAGTGCC
    ATCGAGGGCA TCAAGTACAT CGCAGAGACC ATGAAGTCAG ACCAGGAGTC TAACAATGCG
    GCGGCAGAGT GGAAGTACGT TGCAATGGTG ATGGACCACA TACTCCTCGG AGTCTTCATG
    CTTGTTTGCA TCATCGGAAC CCTAGCCGTG TTTGCAGGTC GACTCATTGA ATTAAATCAG
    CAAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001034612.1
    CDS79..1527
    Translation

    Target ORF information:

    RefSeq Version NM_001039523.3
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001039523.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
    TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG
    CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC
    ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGGGTGAC
    ATGGTAGATC TGCCACGCCC CAGCTGCGTG ACTTTGGGAG TTCCTTTGTT TTCTCATCTG
    CAGAATGAGC AATGGGTGGA TTACAACCTA AAATGGAATC CAGATGACTA TGGCGGTGTG
    AAAAAAATTC ACATTCCTTC AGAAAAGATC TGGCGCCCAG ACCTTGTTCT CTATAACAAT
    GCAGATGGTG ACTTTGCTAT TGTCAAGTTC ACCAAAGTGC TCCTGCAGTA CACTGGCCAC
    ATCACGTGGA CACCTCCAGC CATCTTTAAA AGCTACTGTG AGATCATCGT CACCCACTTT
    CCCTTTGATG AACAGAACTG CAGCATGAAG CTGGGCACCT GGACCTACGA CGGCTCTGTC
    GTGGCCATCA ACCCGGAAAG CGACCAGCCA GACCTGAGCA ACTTCATGGA GAGCGGGGAG
    TGGGTGATCA AGGAGTCCCG GGGCTGGAAG CACTCCGTGA CCTATTCCTG CTGCCCCGAC
    ACCCCCTACC TGGACATCAC CTACCACTTC GTCATGCAGC GCCTGCCCCT CTACTTCATC
    GTCAACGTCA TCATCCCCTG CCTGCTCTTC TCCTTCTTAA CTGGCCTGGT ATTCTACCTG
    CCCACAGACT CAGGGGAGAA GATGACTCTG AGCATCTCTG TCTTACTGTC TTTGACTGTG
    TTCCTTCTGG TCATCGTGGA GCTGATCCCC TCCACGTCCA GTGCTGTGCC CTTGATTGGA
    AAATACATGC TGTTCACCAT GGTGTTCGTC ATTGCCTCCA TCATCATCAC TGTCATCGTC
    ATCAACACAC ACCACCGCTC ACCCAGCACC CATGTCATGC CCAACTGGGT GCGGAAGGTT
    TTTATCGACA CTATCCCAAA TATCATGTTT TTCTCCACAA TGAAAAGACC ATCCAGAGAA
    AAGCAAGACA AAAAGATTTT TACAGAAGAC ATTGATATCT CTGACATTTC TGGAAAGCCA
    GGGCCTCCAC CCATGGGCTT CCACTCTCCC CTGATCAAAC ACCCCGAGGT GAAAAGTGCC
    ATCGAGGGCA TCAAGTACAT CGCAGAGACC ATGAAGTCAG ACCAGGAGTC TAACAATGCG
    GCGGCAGAGT GGAAGTACGT TGCAATGGTG ATGGACCACA TACTCCTCGG AGTCTTCATG
    CTTGTTTGCA TCATCGGAAC CCTAGCCGTG TTTGCAGGTC GACTCATTGA ATTAAATCAG
    CAAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19640
    Clone ID Related Accession (Same CDS sequence) NM_001039523.3 , NM_001039523.2
    Accession Version NM_001039523.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-20
    Organism Homo sapiens(human)
    Product acetylcholine receptor subunit alpha isoform a precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315312.1, CD013889.1 and BC043196.2. This sequence is a reference standard in the RefSeqGene project. On Jun 10, 2008 this sequence version replaced NM_001039523.1. Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.102475.1, AK299445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162946, SAMN03267754 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
    TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG
    CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC
    ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGGGTGAC
    ATGGTAGATC TGCCACGCCC CAGCTGCGTG ACTTTGGGAG TTCCTTTGTT TTCTCATCTG
    CAGAATGAGC AATGGGTGGA TTACAACCTA AAATGGAATC CAGATGACTA TGGCGGTGTG
    AAAAAAATTC ACATTCCTTC AGAAAAGATC TGGCGCCCAG ACCTTGTTCT CTATAACAAT
    GCAGATGGTG ACTTTGCTAT TGTCAAGTTC ACCAAAGTGC TCCTGCAGTA CACTGGCCAC
    ATCACGTGGA CACCTCCAGC CATCTTTAAA AGCTACTGTG AGATCATCGT CACCCACTTT
    CCCTTTGATG AACAGAACTG CAGCATGAAG CTGGGCACCT GGACCTACGA CGGCTCTGTC
    GTGGCCATCA ACCCGGAAAG CGACCAGCCA GACCTGAGCA ACTTCATGGA GAGCGGGGAG
    TGGGTGATCA AGGAGTCCCG GGGCTGGAAG CACTCCGTGA CCTATTCCTG CTGCCCCGAC
    ACCCCCTACC TGGACATCAC CTACCACTTC GTCATGCAGC GCCTGCCCCT CTACTTCATC
    GTCAACGTCA TCATCCCCTG CCTGCTCTTC TCCTTCTTAA CTGGCCTGGT ATTCTACCTG
    CCCACAGACT CAGGGGAGAA GATGACTCTG AGCATCTCTG TCTTACTGTC TTTGACTGTG
    TTCCTTCTGG TCATCGTGGA GCTGATCCCC TCCACGTCCA GTGCTGTGCC CTTGATTGGA
    AAATACATGC TGTTCACCAT GGTGTTCGTC ATTGCCTCCA TCATCATCAC TGTCATCGTC
    ATCAACACAC ACCACCGCTC ACCCAGCACC CATGTCATGC CCAACTGGGT GCGGAAGGTT
    TTTATCGACA CTATCCCAAA TATCATGTTT TTCTCCACAA TGAAAAGACC ATCCAGAGAA
    AAGCAAGACA AAAAGATTTT TACAGAAGAC ATTGATATCT CTGACATTTC TGGAAAGCCA
    GGGCCTCCAC CCATGGGCTT CCACTCTCCC CTGATCAAAC ACCCCGAGGT GAAAAGTGCC
    ATCGAGGGCA TCAAGTACAT CGCAGAGACC ATGAAGTCAG ACCAGGAGTC TAACAATGCG
    GCGGCAGAGT GGAAGTACGT TGCAATGGTG ATGGACCACA TACTCCTCGG AGTCTTCATG
    CTTGTTTGCA TCATCGGAAC CCTAGCCGTG TTTGCAGGTC GACTCATTGA ATTAAATCAG
    CAAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001034612.1
    CDS79..1527
    Translation

    Target ORF information:

    RefSeq Version NM_001039523.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001039523.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
    TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG
    CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC
    ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGGGTGAC
    ATGGTAGATC TGCCACGCCC CAGCTGCGTG ACTTTGGGAG TTCCTTTGTT TTCTCATCTG
    CAGAATGAGC AATGGGTGGA TTACAACCTA AAATGGAATC CAGATGACTA TGGCGGTGTG
    AAAAAAATTC ACATTCCTTC AGAAAAGATC TGGCGCCCAG ACCTTGTTCT CTATAACAAT
    GCAGATGGTG ACTTTGCTAT TGTCAAGTTC ACCAAAGTGC TCCTGCAGTA CACTGGCCAC
    ATCACGTGGA CACCTCCAGC CATCTTTAAA AGCTACTGTG AGATCATCGT CACCCACTTT
    CCCTTTGATG AACAGAACTG CAGCATGAAG CTGGGCACCT GGACCTACGA CGGCTCTGTC
    GTGGCCATCA ACCCGGAAAG CGACCAGCCA GACCTGAGCA ACTTCATGGA GAGCGGGGAG
    TGGGTGATCA AGGAGTCCCG GGGCTGGAAG CACTCCGTGA CCTATTCCTG CTGCCCCGAC
    ACCCCCTACC TGGACATCAC CTACCACTTC GTCATGCAGC GCCTGCCCCT CTACTTCATC
    GTCAACGTCA TCATCCCCTG CCTGCTCTTC TCCTTCTTAA CTGGCCTGGT ATTCTACCTG
    CCCACAGACT CAGGGGAGAA GATGACTCTG AGCATCTCTG TCTTACTGTC TTTGACTGTG
    TTCCTTCTGG TCATCGTGGA GCTGATCCCC TCCACGTCCA GTGCTGTGCC CTTGATTGGA
    AAATACATGC TGTTCACCAT GGTGTTCGTC ATTGCCTCCA TCATCATCAC TGTCATCGTC
    ATCAACACAC ACCACCGCTC ACCCAGCACC CATGTCATGC CCAACTGGGT GCGGAAGGTT
    TTTATCGACA CTATCCCAAA TATCATGTTT TTCTCCACAA TGAAAAGACC ATCCAGAGAA
    AAGCAAGACA AAAAGATTTT TACAGAAGAC ATTGATATCT CTGACATTTC TGGAAAGCCA
    GGGCCTCCAC CCATGGGCTT CCACTCTCCC CTGATCAAAC ACCCCGAGGT GAAAAGTGCC
    ATCGAGGGCA TCAAGTACAT CGCAGAGACC ATGAAGTCAG ACCAGGAGTC TAACAATGCG
    GCGGCAGAGT GGAAGTACGT TGCAATGGTG ATGGACCACA TACTCCTCGG AGTCTTCATG
    CTTGTTTGCA TCATCGGAAC CCTAGCCGTG TTTGCAGGTC GACTCATTGA ATTAAATCAG
    CAAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16431
    Clone ID Related Accession (Same CDS sequence) NM_000079.3 , NM_000079.4
    Accession Version NM_000079.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-25
    Organism Homo sapiens(human)
    Product acetylcholine receptor subunit alpha isoform b precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315312.1, Y00762.1 and BC043196.2. On Jun 10, 2008 this sequence version replaced NM_000079.2. Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (b), that is missing an internal segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.167776.1, SRR1803617.81379.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158800, SAMEA2162946 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
    TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG
    CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC
    ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGCAATGG
    GTGGATTACA ACCTAAAATG GAATCCAGAT GACTATGGCG GTGTGAAAAA AATTCACATT
    CCTTCAGAAA AGATCTGGCG CCCAGACCTT GTTCTCTATA ACAATGCAGA TGGTGACTTT
    GCTATTGTCA AGTTCACCAA AGTGCTCCTG CAGTACACTG GCCACATCAC GTGGACACCT
    CCAGCCATCT TTAAAAGCTA CTGTGAGATC ATCGTCACCC ACTTTCCCTT TGATGAACAG
    AACTGCAGCA TGAAGCTGGG CACCTGGACC TACGACGGCT CTGTCGTGGC CATCAACCCG
    GAAAGCGACC AGCCAGACCT GAGCAACTTC ATGGAGAGCG GGGAGTGGGT GATCAAGGAG
    TCCCGGGGCT GGAAGCACTC CGTGACCTAT TCCTGCTGCC CCGACACCCC CTACCTGGAC
    ATCACCTACC ACTTCGTCAT GCAGCGCCTG CCCCTCTACT TCATCGTCAA CGTCATCATC
    CCCTGCCTGC TCTTCTCCTT CTTAACTGGC CTGGTATTCT ACCTGCCCAC AGACTCAGGG
    GAGAAGATGA CTCTGAGCAT CTCTGTCTTA CTGTCTTTGA CTGTGTTCCT TCTGGTCATC
    GTGGAGCTGA TCCCCTCCAC GTCCAGTGCT GTGCCCTTGA TTGGAAAATA CATGCTGTTC
    ACCATGGTGT TCGTCATTGC CTCCATCATC ATCACTGTCA TCGTCATCAA CACACACCAC
    CGCTCACCCA GCACCCATGT CATGCCCAAC TGGGTGCGGA AGGTTTTTAT CGACACTATC
    CCAAATATCA TGTTTTTCTC CACAATGAAA AGACCATCCA GAGAAAAGCA AGACAAAAAG
    ATTTTTACAG AAGACATTGA TATCTCTGAC ATTTCTGGAA AGCCAGGGCC TCCACCCATG
    GGCTTCCACT CTCCCCTGAT CAAACACCCC GAGGTGAAAA GTGCCATCGA GGGCATCAAG
    TACATCGCAG AGACCATGAA GTCAGACCAG GAGTCTAACA ATGCGGCGGC AGAGTGGAAG
    TACGTTGCAA TGGTGATGGA CCACATACTC CTCGGAGTCT TCATGCTTGT TTGCATCATC
    GGAACCCTAG CCGTGTTTGC AGGTCGACTC ATTGAATTAA ATCAGCAAGG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000070.1
    CDS79..1452
    Translation

    Target ORF information:

    RefSeq Version NM_000079.3
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000079.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
    TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG
    CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC
    ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGCAATGG
    GTGGATTACA ACCTAAAATG GAATCCAGAT GACTATGGCG GTGTGAAAAA AATTCACATT
    CCTTCAGAAA AGATCTGGCG CCCAGACCTT GTTCTCTATA ACAATGCAGA TGGTGACTTT
    GCTATTGTCA AGTTCACCAA AGTGCTCCTG CAGTACACTG GCCACATCAC GTGGACACCT
    CCAGCCATCT TTAAAAGCTA CTGTGAGATC ATCGTCACCC ACTTTCCCTT TGATGAACAG
    AACTGCAGCA TGAAGCTGGG CACCTGGACC TACGACGGCT CTGTCGTGGC CATCAACCCG
    GAAAGCGACC AGCCAGACCT GAGCAACTTC ATGGAGAGCG GGGAGTGGGT GATCAAGGAG
    TCCCGGGGCT GGAAGCACTC CGTGACCTAT TCCTGCTGCC CCGACACCCC CTACCTGGAC
    ATCACCTACC ACTTCGTCAT GCAGCGCCTG CCCCTCTACT TCATCGTCAA CGTCATCATC
    CCCTGCCTGC TCTTCTCCTT CTTAACTGGC CTGGTATTCT ACCTGCCCAC AGACTCAGGG
    GAGAAGATGA CTCTGAGCAT CTCTGTCTTA CTGTCTTTGA CTGTGTTCCT TCTGGTCATC
    GTGGAGCTGA TCCCCTCCAC GTCCAGTGCT GTGCCCTTGA TTGGAAAATA CATGCTGTTC
    ACCATGGTGT TCGTCATTGC CTCCATCATC ATCACTGTCA TCGTCATCAA CACACACCAC
    CGCTCACCCA GCACCCATGT CATGCCCAAC TGGGTGCGGA AGGTTTTTAT CGACACTATC
    CCAAATATCA TGTTTTTCTC CACAATGAAA AGACCATCCA GAGAAAAGCA AGACAAAAAG
    ATTTTTACAG AAGACATTGA TATCTCTGAC ATTTCTGGAA AGCCAGGGCC TCCACCCATG
    GGCTTCCACT CTCCCCTGAT CAAACACCCC GAGGTGAAAA GTGCCATCGA GGGCATCAAG
    TACATCGCAG AGACCATGAA GTCAGACCAG GAGTCTAACA ATGCGGCGGC AGAGTGGAAG
    TACGTTGCAA TGGTGATGGA CCACATACTC CTCGGAGTCT TCATGCTTGT TTGCATCATC
    GGAACCCTAG CCGTGTTTGC AGGTCGACTC ATTGAATTAA ATCAGCAAGG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16431
    Clone ID Related Accession (Same CDS sequence) NM_000079.3 , NM_000079.4
    Accession Version NM_000079.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product acetylcholine receptor subunit alpha isoform b precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315312.1, Y00762.1 and BC043196.2. On Aug 31, 2019 this sequence version replaced NM_000079.3. Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (b), that is missing an internal segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315312.1, Y00762.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000348749.9/ ENSP00000261008.5 RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
    TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG
    CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC
    ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGCAATGG
    GTGGATTACA ACCTAAAATG GAATCCAGAT GACTATGGCG GTGTGAAAAA AATTCACATT
    CCTTCAGAAA AGATCTGGCG CCCAGACCTT GTTCTCTATA ACAATGCAGA TGGTGACTTT
    GCTATTGTCA AGTTCACCAA AGTGCTCCTG CAGTACACTG GCCACATCAC GTGGACACCT
    CCAGCCATCT TTAAAAGCTA CTGTGAGATC ATCGTCACCC ACTTTCCCTT TGATGAACAG
    AACTGCAGCA TGAAGCTGGG CACCTGGACC TACGACGGCT CTGTCGTGGC CATCAACCCG
    GAAAGCGACC AGCCAGACCT GAGCAACTTC ATGGAGAGCG GGGAGTGGGT GATCAAGGAG
    TCCCGGGGCT GGAAGCACTC CGTGACCTAT TCCTGCTGCC CCGACACCCC CTACCTGGAC
    ATCACCTACC ACTTCGTCAT GCAGCGCCTG CCCCTCTACT TCATCGTCAA CGTCATCATC
    CCCTGCCTGC TCTTCTCCTT CTTAACTGGC CTGGTATTCT ACCTGCCCAC AGACTCAGGG
    GAGAAGATGA CTCTGAGCAT CTCTGTCTTA CTGTCTTTGA CTGTGTTCCT TCTGGTCATC
    GTGGAGCTGA TCCCCTCCAC GTCCAGTGCT GTGCCCTTGA TTGGAAAATA CATGCTGTTC
    ACCATGGTGT TCGTCATTGC CTCCATCATC ATCACTGTCA TCGTCATCAA CACACACCAC
    CGCTCACCCA GCACCCATGT CATGCCCAAC TGGGTGCGGA AGGTTTTTAT CGACACTATC
    CCAAATATCA TGTTTTTCTC CACAATGAAA AGACCATCCA GAGAAAAGCA AGACAAAAAG
    ATTTTTACAG AAGACATTGA TATCTCTGAC ATTTCTGGAA AGCCAGGGCC TCCACCCATG
    GGCTTCCACT CTCCCCTGAT CAAACACCCC GAGGTGAAAA GTGCCATCGA GGGCATCAAG
    TACATCGCAG AGACCATGAA GTCAGACCAG GAGTCTAACA ATGCGGCGGC AGAGTGGAAG
    TACGTTGCAA TGGTGATGGA CCACATACTC CTCGGAGTCT TCATGCTTGT TTGCATCATC
    GGAACCCTAG CCGTGTTTGC AGGTCGACTC ATTGAATTAA ATCAGCAAGG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000070.1
    CDS79..1452
    Translation

    Target ORF information:

    RefSeq Version NM_000079.4
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000079.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
    TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG
    CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC
    ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGCAATGG
    GTGGATTACA ACCTAAAATG GAATCCAGAT GACTATGGCG GTGTGAAAAA AATTCACATT
    CCTTCAGAAA AGATCTGGCG CCCAGACCTT GTTCTCTATA ACAATGCAGA TGGTGACTTT
    GCTATTGTCA AGTTCACCAA AGTGCTCCTG CAGTACACTG GCCACATCAC GTGGACACCT
    CCAGCCATCT TTAAAAGCTA CTGTGAGATC ATCGTCACCC ACTTTCCCTT TGATGAACAG
    AACTGCAGCA TGAAGCTGGG CACCTGGACC TACGACGGCT CTGTCGTGGC CATCAACCCG
    GAAAGCGACC AGCCAGACCT GAGCAACTTC ATGGAGAGCG GGGAGTGGGT GATCAAGGAG
    TCCCGGGGCT GGAAGCACTC CGTGACCTAT TCCTGCTGCC CCGACACCCC CTACCTGGAC
    ATCACCTACC ACTTCGTCAT GCAGCGCCTG CCCCTCTACT TCATCGTCAA CGTCATCATC
    CCCTGCCTGC TCTTCTCCTT CTTAACTGGC CTGGTATTCT ACCTGCCCAC AGACTCAGGG
    GAGAAGATGA CTCTGAGCAT CTCTGTCTTA CTGTCTTTGA CTGTGTTCCT TCTGGTCATC
    GTGGAGCTGA TCCCCTCCAC GTCCAGTGCT GTGCCCTTGA TTGGAAAATA CATGCTGTTC
    ACCATGGTGT TCGTCATTGC CTCCATCATC ATCACTGTCA TCGTCATCAA CACACACCAC
    CGCTCACCCA GCACCCATGT CATGCCCAAC TGGGTGCGGA AGGTTTTTAT CGACACTATC
    CCAAATATCA TGTTTTTCTC CACAATGAAA AGACCATCCA GAGAAAAGCA AGACAAAAAG
    ATTTTTACAG AAGACATTGA TATCTCTGAC ATTTCTGGAA AGCCAGGGCC TCCACCCATG
    GGCTTCCACT CTCCCCTGAT CAAACACCCC GAGGTGAAAA GTGCCATCGA GGGCATCAAG
    TACATCGCAG AGACCATGAA GTCAGACCAG GAGTCTAACA ATGCGGCGGC AGAGTGGAAG
    TACGTTGCAA TGGTGATGGA CCACATACTC CTCGGAGTCT TCATGCTTGT TTGCATCATC
    GGAACCCTAG CCGTGTTTGC AGGTCGACTC ATTGAATTAA ATCAGCAAGG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88974
    Clone ID Related Accession (Same CDS sequence) XM_017003256.1
    Accession Version XM_017003256.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product acetylcholine receptor subunit alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTCATGT GTTTGGAGGG TGGGGAGAAG AATCTGACAG TGTTGGTGTC ATCTGCTGTC 
    TCAGCTGGCC TCGTCCTGGG CTCCGAACAT GAGACCCGTC TGGTGGCAAA GCTATTTAAA
    GACTACAGCA GCGTGGTGCG GCCAGTGGAA GACCACCGCC AGGTCGTGGA GGTCACCGTG
    GGCCTGCAGC TGATACAGCT CATCAATGTG GATGAAGTAA ATCAGATCGT GACAACCAAT
    GTGCGTCTGA AACAGGGTGA CATGGTAGAT CTGCCACGCC CCAGCTGCGT GACTTTGGGA
    GTTCCTTTGT TTTCTCATCT GCAGAATGAG CAATGGGTGG ATTACAACCT AAAATGGAAT
    CCAGATGACT ATGGCGGTGT GAAAAAAATT CACATTCCTT CAGAAAAGAT CTGGCGCCCA
    GACCTTGTTC TCTATAACAA TGCAGATGGT GACTTTGCTA TTGTCAAGTT CACCAAAGTG
    CTCCTGCAGT ACACTGGCCA CATCACGTGG ACACCTCCAG CCATCTTTAA AAGCTACTGT
    GAGATCATCG TCACCCACTT TCCCTTTGAT GAACAGAACT GCAGCATGAA GCTGGGCACC
    TGGACCTACG ACGGCTCTGT CGTGGCCATC AACCCGGAAA GCGACCAGCC AGACCTGAGC
    AACTTCATGG AGAGCGGGGA GTGGGTGATC AAGGAGTCCC GGGGCTGGAA GCACTCCGTG
    ACCTATTCCT GCTGCCCCGA CACCCCCTAC CTGGACATCA CCTACCACTT CGTCATGCAG
    CGCCTGCCCC TCTACTTCAT CGTCAACGTC ATCATCCCCT GCCTGCTCTT CTCCTTCTTA
    ACTGGCCTGG TATTCTACCT GCCCACAGAC TCAGGGGAGA AGATGACTCT GAGCATCTCT
    GTCTTACTGT CTTTGACTGT GTTCCTTCTG GTCATCGTGG AGCTGATCCC CTCCACGTCC
    AGTGCTGTGC CCTTGATTGG AAAATACATG CTGTTCACCA TGGTGTTCGT CATTGCCTCC
    ATCATCATCA CTGTCATCGT CATCAACACA CACCACCGCT CACCCAGCAC CCATGTCATG
    CCCAACTGGG TGCGGAAGGT TTTTATCGAC ACTATCCCAA ATATCATGTT TTTCTCCACA
    ATGAAAAGAC CATCCAGAGA AAAGCAAGAC AAAAAGATTT TTACAGAAGA CATTGATATC
    TCTGACATTT CTGGAAAGCC AGGGCCTCCA CCCATGGGCT TCCACTCTCC CCTGATCAAA
    CACCCCGAGG TGAAAAGTGC CATCGAGGGC ATCAAGTACA TCGCAGAGAC CATGAAGTCA
    GACCAGGAGT CTAACAATGC GGCGGCAGAG TGGAAGTACG TTGCAATGGT GATGGACCAC
    ATACTCCTCG GAGTCTTCAT GCTTGTTTGC ATCATCGGAA CCCTAGCCGT GTTTGCAGGT
    CGACTCATTG AATTAAATCA GCAAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858745.1
    CDS228..1697
    Translation

    Target ORF information:

    RefSeq Version XM_017003256.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003256.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTCATGT GTTTGGAGGG TGGGGAGAAG AATCTGACAG TGTTGGTGTC ATCTGCTGTC 
    TCAGCTGGCC TCGTCCTGGG CTCCGAACAT GAGACCCGTC TGGTGGCAAA GCTATTTAAA
    GACTACAGCA GCGTGGTGCG GCCAGTGGAA GACCACCGCC AGGTCGTGGA GGTCACCGTG
    GGCCTGCAGC TGATACAGCT CATCAATGTG GATGAAGTAA ATCAGATCGT GACAACCAAT
    GTGCGTCTGA AACAGGGTGA CATGGTAGAT CTGCCACGCC CCAGCTGCGT GACTTTGGGA
    GTTCCTTTGT TTTCTCATCT GCAGAATGAG CAATGGGTGG ATTACAACCT AAAATGGAAT
    CCAGATGACT ATGGCGGTGT GAAAAAAATT CACATTCCTT CAGAAAAGAT CTGGCGCCCA
    GACCTTGTTC TCTATAACAA TGCAGATGGT GACTTTGCTA TTGTCAAGTT CACCAAAGTG
    CTCCTGCAGT ACACTGGCCA CATCACGTGG ACACCTCCAG CCATCTTTAA AAGCTACTGT
    GAGATCATCG TCACCCACTT TCCCTTTGAT GAACAGAACT GCAGCATGAA GCTGGGCACC
    TGGACCTACG ACGGCTCTGT CGTGGCCATC AACCCGGAAA GCGACCAGCC AGACCTGAGC
    AACTTCATGG AGAGCGGGGA GTGGGTGATC AAGGAGTCCC GGGGCTGGAA GCACTCCGTG
    ACCTATTCCT GCTGCCCCGA CACCCCCTAC CTGGACATCA CCTACCACTT CGTCATGCAG
    CGCCTGCCCC TCTACTTCAT CGTCAACGTC ATCATCCCCT GCCTGCTCTT CTCCTTCTTA
    ACTGGCCTGG TATTCTACCT GCCCACAGAC TCAGGGGAGA AGATGACTCT GAGCATCTCT
    GTCTTACTGT CTTTGACTGT GTTCCTTCTG GTCATCGTGG AGCTGATCCC CTCCACGTCC
    AGTGCTGTGC CCTTGATTGG AAAATACATG CTGTTCACCA TGGTGTTCGT CATTGCCTCC
    ATCATCATCA CTGTCATCGT CATCAACACA CACCACCGCT CACCCAGCAC CCATGTCATG
    CCCAACTGGG TGCGGAAGGT TTTTATCGAC ACTATCCCAA ATATCATGTT TTTCTCCACA
    ATGAAAAGAC CATCCAGAGA AAAGCAAGAC AAAAAGATTT TTACAGAAGA CATTGATATC
    TCTGACATTT CTGGAAAGCC AGGGCCTCCA CCCATGGGCT TCCACTCTCC CCTGATCAAA
    CACCCCGAGG TGAAAAGTGC CATCGAGGGC ATCAAGTACA TCGCAGAGAC CATGAAGTCA
    GACCAGGAGT CTAACAATGC GGCGGCAGAG TGGAAGTACG TTGCAATGGT GATGGACCAC
    ATACTCCTCG GAGTCTTCAT GCTTGTTTGC ATCATCGGAA CCCTAGCCGT GTTTGCAGGT
    CGACTCATTG AATTAAATCA GCAAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88975
    Clone ID Related Accession (Same CDS sequence) XM_017003257.1
    Accession Version XM_017003257.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product acetylcholine receptor subunit alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTTCATGT GTTTGGAGGG TGGGGAGAAG AATCTGACAG TGTTGGTGTC ATCTGCTGTC 
    TCAGCTGGCC TCGTCCTGGG CTCCGAACAT GAGACCCGTC TGGTGGCAAA GCTATTTAAA
    GACTACAGCA GCGTGGTGCG GCCAGTGGAA GACCACCGCC AGGTCGTGGA GGTCACCGTG
    GGCCTGCAGC TGATACAGCT CATCAATGTG GATGAAGTAA ATCAGATCGT GACAACCAAT
    GTGCGTCTGA AACAGCAATG GGTGGATTAC AACCTAAAAT GGAATCCAGA TGACTATGGC
    GGTGTGAAAA AAATTCACAT TCCTTCAGAA AAGATCTGGC GCCCAGACCT TGTTCTCTAT
    AACAATGCAG ATGGTGACTT TGCTATTGTC AAGTTCACCA AAGTGCTCCT GCAGTACACT
    GGCCACATCA CGTGGACACC TCCAGCCATC TTTAAAAGCT ACTGTGAGAT CATCGTCACC
    CACTTTCCCT TTGATGAACA GAACTGCAGC ATGAAGCTGG GCACCTGGAC CTACGACGGC
    TCTGTCGTGG CCATCAACCC GGAAAGCGAC CAGCCAGACC TGAGCAACTT CATGGAGAGC
    GGGGAGTGGG TGATCAAGGA GTCCCGGGGC TGGAAGCACT CCGTGACCTA TTCCTGCTGC
    CCCGACACCC CCTACCTGGA CATCACCTAC CACTTCGTCA TGCAGCGCCT GCCCCTCTAC
    TTCATCGTCA ACGTCATCAT CCCCTGCCTG CTCTTCTCCT TCTTAACTGG CCTGGTATTC
    TACCTGCCCA CAGACTCAGG GGAGAAGATG ACTCTGAGCA TCTCTGTCTT ACTGTCTTTG
    ACTGTGTTCC TTCTGGTCAT CGTGGAGCTG ATCCCCTCCA CGTCCAGTGC TGTGCCCTTG
    ATTGGAAAAT ACATGCTGTT CACCATGGTG TTCGTCATTG CCTCCATCAT CATCACTGTC
    ATCGTCATCA ACACACACCA CCGCTCACCC AGCACCCATG TCATGCCCAA CTGGGTGCGG
    AAGGTTTTTA TCGACACTAT CCCAAATATC ATGTTTTTCT CCACAATGAA AAGACCATCC
    AGAGAAAAGC AAGACAAAAA GATTTTTACA GAAGACATTG ATATCTCTGA CATTTCTGGA
    AAGCCAGGGC CTCCACCCAT GGGCTTCCAC TCTCCCCTGA TCAAACACCC CGAGGTGAAA
    AGTGCCATCG AGGGCATCAA GTACATCGCA GAGACCATGA AGTCAGACCA GGAGTCTAAC
    AATGCGGCGG CAGAGTGGAA GTACGTTGCA ATGGTGATGG ACCACATACT CCTCGGAGTC
    TTCATGCTTG TTTGCATCAT CGGAACCCTA GCCGTGTTTG CAGGTCGACT CATTGAATTA
    AATCAGCAAG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858746.1
    CDS225..1619
    Translation

    Target ORF information:

    RefSeq Version XM_017003257.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017003257.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTTCATGT GTTTGGAGGG TGGGGAGAAG AATCTGACAG TGTTGGTGTC ATCTGCTGTC 
    TCAGCTGGCC TCGTCCTGGG CTCCGAACAT GAGACCCGTC TGGTGGCAAA GCTATTTAAA
    GACTACAGCA GCGTGGTGCG GCCAGTGGAA GACCACCGCC AGGTCGTGGA GGTCACCGTG
    GGCCTGCAGC TGATACAGCT CATCAATGTG GATGAAGTAA ATCAGATCGT GACAACCAAT
    GTGCGTCTGA AACAGCAATG GGTGGATTAC AACCTAAAAT GGAATCCAGA TGACTATGGC
    GGTGTGAAAA AAATTCACAT TCCTTCAGAA AAGATCTGGC GCCCAGACCT TGTTCTCTAT
    AACAATGCAG ATGGTGACTT TGCTATTGTC AAGTTCACCA AAGTGCTCCT GCAGTACACT
    GGCCACATCA CGTGGACACC TCCAGCCATC TTTAAAAGCT ACTGTGAGAT CATCGTCACC
    CACTTTCCCT TTGATGAACA GAACTGCAGC ATGAAGCTGG GCACCTGGAC CTACGACGGC
    TCTGTCGTGG CCATCAACCC GGAAAGCGAC CAGCCAGACC TGAGCAACTT CATGGAGAGC
    GGGGAGTGGG TGATCAAGGA GTCCCGGGGC TGGAAGCACT CCGTGACCTA TTCCTGCTGC
    CCCGACACCC CCTACCTGGA CATCACCTAC CACTTCGTCA TGCAGCGCCT GCCCCTCTAC
    TTCATCGTCA ACGTCATCAT CCCCTGCCTG CTCTTCTCCT TCTTAACTGG CCTGGTATTC
    TACCTGCCCA CAGACTCAGG GGAGAAGATG ACTCTGAGCA TCTCTGTCTT ACTGTCTTTG
    ACTGTGTTCC TTCTGGTCAT CGTGGAGCTG ATCCCCTCCA CGTCCAGTGC TGTGCCCTTG
    ATTGGAAAAT ACATGCTGTT CACCATGGTG TTCGTCATTG CCTCCATCAT CATCACTGTC
    ATCGTCATCA ACACACACCA CCGCTCACCC AGCACCCATG TCATGCCCAA CTGGGTGCGG
    AAGGTTTTTA TCGACACTAT CCCAAATATC ATGTTTTTCT CCACAATGAA AAGACCATCC
    AGAGAAAAGC AAGACAAAAA GATTTTTACA GAAGACATTG ATATCTCTGA CATTTCTGGA
    AAGCCAGGGC CTCCACCCAT GGGCTTCCAC TCTCCCCTGA TCAAACACCC CGAGGTGAAA
    AGTGCCATCG AGGGCATCAA GTACATCGCA GAGACCATGA AGTCAGACCA GGAGTCTAAC
    AATGCGGCGG CAGAGTGGAA GTACGTTGCA ATGGTGATGG ACCACATACT CCTCGGAGTC
    TTCATGCTTG TTTGCATCAT CGGAACCCTA GCCGTGTTTG CAGGTCGACT CATTGAATTA
    AATCAGCAAG GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.