U2AF2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	U2AF2
Entrez Gene ID	11338
Full Name	U2 small nuclear RNA auxiliary factor 2
Synonyms	U2AF65
General protein information	Preferred Names U2 small nuclear RNA auxiliary factor 2 Names splicing factor U2AF 65 kDa subunit U2 (RNU2) small nuclear RNA auxiliary factor 2 U2 auxiliary factor 65 kDa subunit U2 small nuclear ribonucleoprotein auxiliary factor (65kD) U2 snRNP auxiliary factor large subunit hU2AF65
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.42
Summary	U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq, Jul 2008].
Disorder MIM:	191318

mRNA and Protein(s)

mRNA	Protein	Name
XM_006722994.2	XP_006723057.1	splicing factor U2AF 65 kDa subunit isoform X2
NM_001012478.1	NP_001012496.1	splicing factor U2AF 65 kDa subunit isoform b
NM_007279.2	NP_009210.1	splicing factor U2AF 65 kDa subunit isoform a
XM_011526410.1	XP_011524712.1	splicing factor U2AF 65 kDa subunit isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	U2AF2	NP_009210.1
Mus musculus (house mouse)	U2af2	NP_598432.2
Danio rerio (zebrafish)	u2af2b	NP_991252.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002908	XP_311994.4
Bos taurus (cattle)	U2AF2	NP_001068804.1
Neurospora crassa	NCU03039	XP_964044.1
Arabidopsis thaliana (thale cress)	ATU2AF65A	NP_001190937.1
Oryza sativa (rice)	Os11g0682300	NP_001068459.1
Canis lupus familiaris (dog)	U2AF2	XP_005616162.1
Arabidopsis thaliana (thale cress)	AT1G60900	NP_176287.3
Oryza sativa (rice)	Os11g0636900	NP_001068330.1
Xenopus tropicalis (tropical clawed frog)	u2af2	NP_001016998.1
Rattus norvegicus (Norway rat)	U2af2	XP_006228382.1
Magnaporthe oryzae (rice blast fungus)	MGG_00348	XP_003718735.1
Danio rerio (zebrafish)	u2af2a	NP_001025127.1
Drosophila melanogaster (fruit fly)	U2af50	NP_476891.1
Caenorhabditis elegans (roundworm)	uaf-1	NP_001022967.1
Schizosaccharomyces pombe (fission yeast)	prp2	NP_595396.1

Gene Ontology
Bibliography

Related articles in PubMed

Chromatin environment, transcriptional regulation, and splicing distinguish lincRNAs and mRNAs.
Mel? M, Mattioli K, Mallard W, Shechner DM, Gerhardinger C, Rinn JL
Genome research27(1)27-37(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

JMJD6 regulated a large set of alternative splicing events, and importantly, its regulated splicing events were significantly overlapped those controlled by U2AF65.
Title: JMJD6 and U2AF65 co-regulate alternative splicing in both JMJD6 enzymatic activity dependent and independent manner.

the most discriminant differences between lincRNAs and mRNAs involve splicing. LincRNAs are less efficiently spliced, which cannot be explained by differences in U1 binding or the density of exonic splicing enhancers but may be partially attributed to lower U2AF65 binding and weaker splicing-related motifs.
Title: Chromatin environment, transcriptional regulation, and splicing distinguish lincRNAs and mRNAs.

These findings uncovered a hitherto unappreciated function of CD82 in severing the linkage between U2AF2-mediated CD44 alternative splicing and cancer aggressiveness, with potential prognostic and therapeutic implications in melanoma
Title: CD82 suppresses CD44 alternative splicing-dependent melanoma metastasis by mediating U2AF2 ubiquitination and degradation.

RBM39 is extensively involved in alternative splicing of RNA and helps regulate transcript levels. RBM39 may modulate alternative splicing similarly to U2AF65 by either directly binding to RNA or recruiting other splicing factors, such as U2AF65.
Title: Global regulation of alternative RNA splicing by the SR-rich protein RBM39.

This study therefore establishes a structural basis for specific UHM-ULM interactions by splicing factors such as U2AF35, U2AF65, RBM39 and SF3b155, and a platform for continued studies of intermolecular interactions governing disease-related alternative splicing in eukaryotic cells.
Title: UHM-ULM interactions in the RBM39-U2AF65 splicing-factor complex.

The following U2AF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the U2AF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13293	NM_001012478.1 Latest version!	Homo sapiens U2 small nuclear RNA auxiliary factor 2 (U2AF2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu17874	NM_007279.2 Latest version!	Homo sapiens U2 small nuclear RNA auxiliary factor 2 (U2AF2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$199.50-$279.30 $399.00
OHu51983	XM_006722994.2 Latest version!	Homo sapiens U2 small nuclear RNA auxiliary factor 2 (U2AF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu64926	XM_011526410.1 Latest version!	Homo sapiens U2 small nuclear RNA auxiliary factor 2 (U2AF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

U2AF2 ( NM_001012478.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001012496.1 Homo sapiens U2 small nuclear RNA auxiliary factor 2 (U2AF2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13293
Clone ID Related Accession (Same CDS sequence)	NM_001012478.1
Accession Version	NM_001012478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	splicing factor U2AF 65 kDa subunit isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN364862.1, BC030574.2 and BC008740.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC030574.2, SRR1163657.335043.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGACT TCGACGAGTT CGAGCGGCAG CTCAACGAGA ATAAACAAGA GCGGGACAAG 
GAGAACCGGC ATCGGAAGCG CAGCCACAGC CGCTCTCGGA GCCGGGACCG CAAACGCCGG 
AGCCGGAGCC GCGACCGGCG CAACCGGGAC CAGCGGAGCG CCTCCCGGGA CAGGCGACGA 
CGCAGCAAAC CTTTGACCAG AGGCGCTAAA GAGGAGCACG GTGGACTGAT TCGTTCCCCC 
CGCCACGAGA AGAAGAAGAA GGTCCGTAAA TACTGGGACG TGCCACCCCC AGGCTTTGAG 
CACATCACCC CAATGCAGTA CAAGGCCATG CAAGCTGCGG GTCAGATTCC AGCCACTGCT 
CTTCTCCCCA CCATGACCCC TGACGGTCTG GCTGTGACCC CAACGCCGGT GCCCGTGGTC 
GGGAGCCAGA TGACCAGACA AGCCCGGCGC CTCTACGTGG GCAACATCCC CTTTGGCATC 
ACTGAGGAGG CCATGATGGA TTTCTTCAAC GCCCAGATGC GCCTGGGGGG GCTGACCCAG 
GCCCCTGGCA ACCCAGTGTT GGCTGTGCAG ATTAACCAGG ACAAGAATTT TGCCTTTTTG 
GAGTTCCGCT CAGTGGACGA GACTACCCAG GCTATGGCCT TTGATGGCAT CATCTTCCAG 
GGCCAGTCAC TAAAGATCCG CAGGCCTCAC GACTACCAGC CGCTTCCTGG CATGTCAGAG 
AACCCCTCCG TCTATGTGCC TGGGGTTGTG TCCACTGTGG TCCCCGACTC TGCCCACAAG 
CTGTTCATCG GGGGCTTACC CAACTACCTG AACGATGACC AGGTCAAAGA GCTGCTGACA 
TCCTTTGGGC CCCTCAAGGC CTTCAACCTG GTCAAGGACA GTGCCACGGG GCTCTCCAAG 
GGCTACGCCT TCTGTGAGTA CGTGGACATC AACGTCACGG ATCAGGCCAT TGCGGGGCTG 
AACGGCATGC AGCTGGGGGA TAAGAAGCTG CTGGTCCAGA GGGCGAGTGT GGGAGCCAAG 
AATGCCACGC TGAGCACCAT CAATCAGACG CCTGTGACCC TGCAAGTGCC GGGCTTGATG 
AGCTCCCAGG TGCAGATGGG CGGCCACCCG ACTGAGGTCC TGTGCCTCAT GAACATGGTG 
CTGCCTGAGG AGCTGCTGGA CGACGAGGAG TATGAGGAGA TCGTGGAGGA TGTGCGGGAC 
GAGTGCAGCA AGTACGGGCT TGTCAAGTCC ATCGAGATCC CCCGGCCTGT GGACGGCGTC 
GAGGTGCCCG GCTGCGGAAA GATCTTTGTG GAGTTCACCT CTGTGTTTGA CTGCCAGAAA 
GCCATGCAGG GCCTGACGGG CCGCAAGTTC GCCAACAGAG TGGTTGTCAC AAAATACTGT 
GACCCCGACT CTTATCACCG CCGGGACTTC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012496.1
CDS	1056..2471
Translation	MSDFDEFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRDRRNRDQRSASRDRRRRSKPLTRGAKEEHGGLIRSPRHEKKKKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW

Target ORF information:

RefSeq Version	NM_001012478.1
Organism	Homo sapiens(human)
Definition	Homo sapiens U2 small nuclear RNA auxiliary factor 2 (U2AF2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012478.1

ORF Insert Sequence:

ATGTCGGACT TCGACGAGTT CGAGCGGCAG CTCAACGAGA ATAAACAAGA GCGGGACAAG 
GAGAACCGGC ATCGGAAGCG CAGCCACAGC CGCTCTCGGA GCCGGGACCG CAAACGCCGG 
AGCCGGAGCC GCGACCGGCG CAACCGGGAC CAGCGGAGCG CCTCCCGGGA CAGGCGACGA 
CGCAGCAAAC CTTTGACCAG AGGCGCTAAA GAGGAGCACG GTGGACTGAT TCGTTCCCCC 
CGCCACGAGA AGAAGAAGAA GGTCCGTAAA TACTGGGACG TGCCACCCCC AGGCTTTGAG 
CACATCACCC CAATGCAGTA CAAGGCCATG CAAGCTGCGG GTCAGATTCC AGCCACTGCT 
CTTCTCCCCA CCATGACCCC TGACGGTCTG GCTGTGACCC CAACGCCGGT GCCCGTGGTC 
GGGAGCCAGA TGACCAGACA AGCCCGGCGC CTCTACGTGG GCAACATCCC CTTTGGCATC 
ACTGAGGAGG CCATGATGGA TTTCTTCAAC GCCCAGATGC GCCTGGGGGG GCTGACCCAG 
GCCCCTGGCA ACCCAGTGTT GGCTGTGCAG ATTAACCAGG ACAAGAATTT TGCCTTTTTG 
GAGTTCCGCT CAGTGGACGA GACTACCCAG GCTATGGCCT TTGATGGCAT CATCTTCCAG 
GGCCAGTCAC TAAAGATCCG CAGGCCTCAC GACTACCAGC CGCTTCCTGG CATGTCAGAG 
AACCCCTCCG TCTATGTGCC TGGGGTTGTG TCCACTGTGG TCCCCGACTC TGCCCACAAG 
CTGTTCATCG GGGGCTTACC CAACTACCTG AACGATGACC AGGTCAAAGA GCTGCTGACA 
TCCTTTGGGC CCCTCAAGGC CTTCAACCTG GTCAAGGACA GTGCCACGGG GCTCTCCAAG 
GGCTACGCCT TCTGTGAGTA CGTGGACATC AACGTCACGG ATCAGGCCAT TGCGGGGCTG 
AACGGCATGC AGCTGGGGGA TAAGAAGCTG CTGGTCCAGA GGGCGAGTGT GGGAGCCAAG 
AATGCCACGC TGAGCACCAT CAATCAGACG CCTGTGACCC TGCAAGTGCC GGGCTTGATG 
AGCTCCCAGG TGCAGATGGG CGGCCACCCG ACTGAGGTCC TGTGCCTCAT GAACATGGTG 
CTGCCTGAGG AGCTGCTGGA CGACGAGGAG TATGAGGAGA TCGTGGAGGA TGTGCGGGAC 
GAGTGCAGCA AGTACGGGCT TGTCAAGTCC ATCGAGATCC CCCGGCCTGT GGACGGCGTC 
GAGGTGCCCG GCTGCGGAAA GATCTTTGTG GAGTTCACCT CTGTGTTTGA CTGCCAGAAA 
GCCATGCAGG GCCTGACGGG CCGCAAGTTC GCCAACAGAG TGGTTGTCAC AAAATACTGT 
GACCCCGACT CTTATCACCG CCGGGACTTC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

U2AF2 ( NM_007279.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_009210.1 Homo sapiens U2 small nuclear RNA auxiliary factor 2 (U2AF2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17874
Clone ID Related Accession (Same CDS sequence)	NM_007279.2
Accession Version	NM_007279.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	splicing factor U2AF 65 kDa subunit isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN364862.1, BC030574.2, X64044.1 and BC008740.2. This sequence is a reference standard in the RefSeqGene project. On Feb 24, 2005 this sequence version replaced NM_007279.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.233026.1, SRR1163658.499689.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGGACT TCGACGAGTT CGAGCGGCAG CTCAACGAGA ATAAACAAGA GCGGGACAAG 
GAGAACCGGC ATCGGAAGCG CAGCCACAGC CGCTCTCGGA GCCGGGACCG CAAACGCCGG 
AGCCGGAGCC GCGACCGGCG CAACCGGGAC CAGCGGAGCG CCTCCCGGGA CAGGCGACGA 
CGCAGCAAAC CTTTGACCAG AGGCGCTAAA GAGGAGCACG GTGGACTGAT TCGTTCCCCC 
CGCCACGAGA AGAAGAAGAA GGTCCGTAAA TACTGGGACG TGCCACCCCC AGGCTTTGAG 
CACATCACCC CAATGCAGTA CAAGGCCATG CAAGCTGCGG GTCAGATTCC AGCCACTGCT 
CTTCTCCCCA CCATGACCCC TGACGGTCTG GCTGTGACCC CAACGCCGGT GCCCGTGGTC 
GGGAGCCAGA TGACCAGACA AGCCCGGCGC CTCTACGTGG GCAACATCCC CTTTGGCATC 
ACTGAGGAGG CCATGATGGA TTTCTTCAAC GCCCAGATGC GCCTGGGGGG GCTGACCCAG 
GCCCCTGGCA ACCCAGTGTT GGCTGTGCAG ATTAACCAGG ACAAGAATTT TGCCTTTTTG 
GAGTTCCGCT CAGTGGACGA GACTACCCAG GCTATGGCCT TTGATGGCAT CATCTTCCAG 
GGCCAGTCAC TAAAGATCCG CAGGCCTCAC GACTACCAGC CGCTTCCTGG CATGTCAGAG 
AACCCCTCCG TCTATGTGCC TGGGGTTGTG TCCACTGTGG TCCCCGACTC TGCCCACAAG 
CTGTTCATCG GGGGCTTACC CAACTACCTG AACGATGACC AGGTCAAAGA GCTGCTGACA 
TCCTTTGGGC CCCTCAAGGC CTTCAACCTG GTCAAGGACA GTGCCACGGG GCTCTCCAAG 
GGCTACGCCT TCTGTGAGTA CGTGGACATC AACGTCACGG ATCAGGCCAT TGCGGGGCTG 
AACGGCATGC AGCTGGGGGA TAAGAAGCTG CTGGTCCAGA GGGCGAGTGT GGGAGCCAAG 
AATGCCACGC TGGTGAGCCC CCCGAGCACC ATCAATCAGA CGCCTGTGAC CCTGCAAGTG 
CCGGGCTTGA TGAGCTCCCA GGTGCAGATG GGCGGCCACC CGACTGAGGT CCTGTGCCTC 
ATGAACATGG TGCTGCCTGA GGAGCTGCTG GACGACGAGG AGTATGAGGA GATCGTGGAG 
GATGTGCGGG ACGAGTGCAG CAAGTACGGG CTTGTCAAGT CCATCGAGAT CCCCCGGCCT 
GTGGACGGCG TCGAGGTGCC CGGCTGCGGA AAGATCTTTG TGGAGTTCAC CTCTGTGTTT 
GACTGCCAGA AAGCCATGCA GGGCCTGACG GGCCGCAAGT TCGCCAACAG AGTGGTTGTC 
ACAAAATACT GTGACCCCGA CTCTTATCAC CGCCGGGACT TCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009210.1
CDS	1056..2483
Translation	MSDFDEFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRDRRNRDQRSASRDRRRRSKPLTRGAKEEHGGLIRSPRHEKKKKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW

Target ORF information:

RefSeq Version	NM_007279.2
Organism	Homo sapiens(human)
Definition	Homo sapiens U2 small nuclear RNA auxiliary factor 2 (U2AF2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007279.2

ORF Insert Sequence:

ATGTCGGACT TCGACGAGTT CGAGCGGCAG CTCAACGAGA ATAAACAAGA GCGGGACAAG 
GAGAACCGGC ATCGGAAGCG CAGCCACAGC CGCTCTCGGA GCCGGGACCG CAAACGCCGG 
AGCCGGAGCC GCGACCGGCG CAACCGGGAC CAGCGGAGCG CCTCCCGGGA CAGGCGACGA 
CGCAGCAAAC CTTTGACCAG AGGCGCTAAA GAGGAGCACG GTGGACTGAT TCGTTCCCCC 
CGCCACGAGA AGAAGAAGAA GGTCCGTAAA TACTGGGACG TGCCACCCCC AGGCTTTGAG 
CACATCACCC CAATGCAGTA CAAGGCCATG CAAGCTGCGG GTCAGATTCC AGCCACTGCT 
CTTCTCCCCA CCATGACCCC TGACGGTCTG GCTGTGACCC CAACGCCGGT GCCCGTGGTC 
GGGAGCCAGA TGACCAGACA AGCCCGGCGC CTCTACGTGG GCAACATCCC CTTTGGCATC 
ACTGAGGAGG CCATGATGGA TTTCTTCAAC GCCCAGATGC GCCTGGGGGG GCTGACCCAG 
GCCCCTGGCA ACCCAGTGTT GGCTGTGCAG ATTAACCAGG ACAAGAATTT TGCCTTTTTG 
GAGTTCCGCT CAGTGGACGA GACTACCCAG GCTATGGCCT TTGATGGCAT CATCTTCCAG 
GGCCAGTCAC TAAAGATCCG CAGGCCTCAC GACTACCAGC CGCTTCCTGG CATGTCAGAG 
AACCCCTCCG TCTATGTGCC TGGGGTTGTG TCCACTGTGG TCCCCGACTC TGCCCACAAG 
CTGTTCATCG GGGGCTTACC CAACTACCTG AACGATGACC AGGTCAAAGA GCTGCTGACA 
TCCTTTGGGC CCCTCAAGGC CTTCAACCTG GTCAAGGACA GTGCCACGGG GCTCTCCAAG 
GGCTACGCCT TCTGTGAGTA CGTGGACATC AACGTCACGG ATCAGGCCAT TGCGGGGCTG 
AACGGCATGC AGCTGGGGGA TAAGAAGCTG CTGGTCCAGA GGGCGAGTGT GGGAGCCAAG 
AATGCCACGC TGGTGAGCCC CCCGAGCACC ATCAATCAGA CGCCTGTGAC CCTGCAAGTG 
CCGGGCTTGA TGAGCTCCCA GGTGCAGATG GGCGGCCACC CGACTGAGGT CCTGTGCCTC 
ATGAACATGG TGCTGCCTGA GGAGCTGCTG GACGACGAGG AGTATGAGGA GATCGTGGAG 
GATGTGCGGG ACGAGTGCAG CAAGTACGGG CTTGTCAAGT CCATCGAGAT CCCCCGGCCT 
GTGGACGGCG TCGAGGTGCC CGGCTGCGGA AAGATCTTTG TGGAGTTCAC CTCTGTGTTT 
GACTGCCAGA AAGCCATGCA GGGCCTGACG GGCCGCAAGT TCGCCAACAG AGTGGTTGTC 
ACAAAATACT GTGACCCCGA CTCTTATCAC CGCCGGGACT TCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

U2AF2 ( XM_006722994.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723057.1 Homo sapiens U2 small nuclear RNA auxiliary factor 2 (U2AF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu51983
Clone ID Related Accession (Same CDS sequence)	XM_006722994.2
Accession Version	XM_006722994.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	splicing factor U2AF 65 kDa subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006722994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATT TCTTCAACGC CCAGATGCGC CTGGGGGGGC TGACCCAGGC CCCTGGCAAC 
CCAGTGTTGG CTGTGCAGAT TAACCAGGAC AAGAATTTTG CCTTTTTGGA GTTCCGCTCA 
GTGGACGAGA CTACCCAGGC TATGGCCTTT GATGGCATCA TCTTCCAGGG CCAGTCACTA 
AAGATCCGCA GGCCTCACGA CTACCAGCCG CTTCCTGGCA TGTCAGAGAA CCCCTCCGTC 
TATGTGCCTG GGGTTGTGTC CACTGTGGTC CCCGACTCTG CCCACAAGCT GTTCATCGGG 
GGCTTACCCA ACTACCTGAA CGATGACCAG GTCAAAGAGC TGCTGACATC CTTTGGGCCC 
CTCAAGGCCT TCAACCTGGT CAAGGACAGT GCCACGGGGC TCTCCAAGGG CTACGCCTTC 
TGTGAGTACG TGGACATCAA CGTCACGGAT CAGGCCATTG CGGGGCTGAA CGGCATGCAG 
CTGGGGGATA AGAAGCTGCT GGTCCAGAGG GCGAGTGTGG GAGCCAAGAA TGCCACGCTG 
GTGAGCCCCC CGAGCACCAT CAATCAGACG CCTGTGACCC TGCAAGTGCC GGGCTTGATG 
AGCTCCCAGG TGCAGATGGG CGGCCACCCG ACTGAGGTCC TGTGCCTCAT GAACATGGTG 
CTGCCTGAGG AGCTGCTGGA CGACGAGGAG TATGAGGAGA TCGTGGAGGA CGTGCGGGAC 
GAGTGCAGCA AGTACGGGCT TGTCAAGTCC ATCGAGATCC CCCGGCCTGT GGACGGCGTC 
GAGGTGCCCG GCTGCGGAAA GATCTTTGTG GAGTTCACCT CTGTGTTTGA CTGCCAGAAA 
GCCATGCAGG GCCTGACGGG CCGCAAGTTC GCCAACAGAG TGGTTGTCAC AAAATACTGT 
GACCCCGACT CTTATCACCG CCGGGACTTC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723057.1
CDS	1646..2581
Translation	MMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW

Target ORF information:

RefSeq Version	XM_006722994.2
Organism	Homo sapiens(human)
Definition	Homo sapiens U2 small nuclear RNA auxiliary factor 2 (U2AF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722994.2

ORF Insert Sequence:

ATGATGGATT TCTTCAACGC CCAGATGCGC CTGGGGGGGC TGACCCAGGC CCCTGGCAAC 
CCAGTGTTGG CTGTGCAGAT TAACCAGGAC AAGAATTTTG CCTTTTTGGA GTTCCGCTCA 
GTGGACGAGA CTACCCAGGC TATGGCCTTT GATGGCATCA TCTTCCAGGG CCAGTCACTA 
AAGATCCGCA GGCCTCACGA CTACCAGCCG CTTCCTGGCA TGTCAGAGAA CCCCTCCGTC 
TATGTGCCTG GGGTTGTGTC CACTGTGGTC CCCGACTCTG CCCACAAGCT GTTCATCGGG 
GGCTTACCCA ACTACCTGAA CGATGACCAG GTCAAAGAGC TGCTGACATC CTTTGGGCCC 
CTCAAGGCCT TCAACCTGGT CAAGGACAGT GCCACGGGGC TCTCCAAGGG CTACGCCTTC 
TGTGAGTACG TGGACATCAA CGTCACGGAT CAGGCCATTG CGGGGCTGAA CGGCATGCAG 
CTGGGGGATA AGAAGCTGCT GGTCCAGAGG GCGAGTGTGG GAGCCAAGAA TGCCACGCTG 
GTGAGCCCCC CGAGCACCAT CAATCAGACG CCTGTGACCC TGCAAGTGCC GGGCTTGATG 
AGCTCCCAGG TGCAGATGGG CGGCCACCCG ACTGAGGTCC TGTGCCTCAT GAACATGGTG 
CTGCCTGAGG AGCTGCTGGA CGACGAGGAG TATGAGGAGA TCGTGGAGGA CGTGCGGGAC 
GAGTGCAGCA AGTACGGGCT TGTCAAGTCC ATCGAGATCC CCCGGCCTGT GGACGGCGTC 
GAGGTGCCCG GCTGCGGAAA GATCTTTGTG GAGTTCACCT CTGTGTTTGA CTGCCAGAAA 
GCCATGCAGG GCCTGACGGG CCGCAAGTTC GCCAACAGAG TGGTTGTCAC AAAATACTGT 
GACCCCGACT CTTATCACCG CCGGGACTTC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

U2AF2 ( XM_011526410.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011524712.1 Homo sapiens U2 small nuclear RNA auxiliary factor 2 (U2AF2), transcript variant X1, mRNA.

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CloneID	OHu64926
Clone ID Related Accession (Same CDS sequence)	XM_011526410.1
Accession Version	XM_011526410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	splicing factor U2AF 65 kDa subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTACA AGGCCATGCA AGCTGCGGGT CAGATTCCAG CCACTGCTCT TCTCCCCACC 
ATGACCCCTG ACGGTCTGGC TGTGACCCCA ACGCCGGTGC CCGTGGTCGG GAGCCAGATG 
ACCAGACAAG CCCGGCGCCT CTACGTGGGC AACATCCCCT TTGGCATCAC TGAGGAGGCC 
ATGATGGATT TCTTCAACGC CCAGATGCGC CTGGGGGGGC TGACCCAGGC CCCTGGCAAC 
CCAGTGTTGG CTGTGCAGAT TAACCAGGAC AAGAATTTTG CCTTTTTGGA GTTCCGCTCA 
GTGGACGAGA CTACCCAGGC TATGGCCTTT GATGGCATCA TCTTCCAGGG CCAGTCACTA 
AAGATCCGCA GGCCTCACGA CTACCAGCCG CTTCCTGGCA TGTCAGAGAA CCCCTCCGTC 
TATGTGCCTG GGGTTGTGTC CACTGTGGTC CCCGACTCTG CCCACAAGCT GTTCATCGGG 
GGCTTACCCA ACTACCTGAA CGATGACCAG GTCAAAGAGC TGCTGACATC CTTTGGGCCC 
CTCAAGGCCT TCAACCTGGT CAAGGACAGT GCCACGGGGC TCTCCAAGGG CTACGCCTTC 
TGTGAGTACG TGGACATCAA CGTCACGGAT CAGGCCATTG CGGGGCTGAA CGGCATGCAG 
CTGGGGGATA AGAAGCTGCT GGTCCAGAGG GCGAGTGTGG GAGCCAAGAA TGCCACGCTG 
GTGAGCCCCC CGAGCACCAT CAATCAGACG CCTGTGACCC TGCAAGTGCC GGGCTTGATG 
AGCTCCCAGG TGCAGATGGG CGGCCACCCG ACTGAGGTCC TGTGCCTCAT GAACATGGTG 
CTGCCTGAGG AGCTGCTGGA CGACGAGGAG TATGAGGAGA TCGTGGAGGA CGTGCGGGAC 
GAGTGCAGCA AGTACGGGCT TGTCAAGTCC ATCGAGATCC CCCGGCCTGT GGACGGCGTC 
GAGGTGCCCG GCTGCGGAAA GATCTTTGTG GAGTTCACCT CTGTGTTTGA CTGCCAGAAA 
GCCATGCAGG GCCTGACGGG CCGCAAGTTC GCCAACAGAG TGGTTGTCAC AAAATACTGT 
GACCCCGACT CTTATCACCG CCGGGACTTC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011524712.1
CDS	1406..2521
Translation	MQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW

Target ORF information:

RefSeq Version	XM_011526410.1
Organism	Homo sapiens(human)
Definition	Homo sapiens U2 small nuclear RNA auxiliary factor 2 (U2AF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526410.1

ORF Insert Sequence:

ATGCAGTACA AGGCCATGCA AGCTGCGGGT CAGATTCCAG CCACTGCTCT TCTCCCCACC 
ATGACCCCTG ACGGTCTGGC TGTGACCCCA ACGCCGGTGC CCGTGGTCGG GAGCCAGATG 
ACCAGACAAG CCCGGCGCCT CTACGTGGGC AACATCCCCT TTGGCATCAC TGAGGAGGCC 
ATGATGGATT TCTTCAACGC CCAGATGCGC CTGGGGGGGC TGACCCAGGC CCCTGGCAAC 
CCAGTGTTGG CTGTGCAGAT TAACCAGGAC AAGAATTTTG CCTTTTTGGA GTTCCGCTCA 
GTGGACGAGA CTACCCAGGC TATGGCCTTT GATGGCATCA TCTTCCAGGG CCAGTCACTA 
AAGATCCGCA GGCCTCACGA CTACCAGCCG CTTCCTGGCA TGTCAGAGAA CCCCTCCGTC 
TATGTGCCTG GGGTTGTGTC CACTGTGGTC CCCGACTCTG CCCACAAGCT GTTCATCGGG 
GGCTTACCCA ACTACCTGAA CGATGACCAG GTCAAAGAGC TGCTGACATC CTTTGGGCCC 
CTCAAGGCCT TCAACCTGGT CAAGGACAGT GCCACGGGGC TCTCCAAGGG CTACGCCTTC 
TGTGAGTACG TGGACATCAA CGTCACGGAT CAGGCCATTG CGGGGCTGAA CGGCATGCAG 
CTGGGGGATA AGAAGCTGCT GGTCCAGAGG GCGAGTGTGG GAGCCAAGAA TGCCACGCTG 
GTGAGCCCCC CGAGCACCAT CAATCAGACG CCTGTGACCC TGCAAGTGCC GGGCTTGATG 
AGCTCCCAGG TGCAGATGGG CGGCCACCCG ACTGAGGTCC TGTGCCTCAT GAACATGGTG 
CTGCCTGAGG AGCTGCTGGA CGACGAGGAG TATGAGGAGA TCGTGGAGGA CGTGCGGGAC 
GAGTGCAGCA AGTACGGGCT TGTCAAGTCC ATCGAGATCC CCCGGCCTGT GGACGGCGTC 
GAGGTGCCCG GCTGCGGAAA GATCTTTGTG GAGTTCACCT CTGTGTTTGA CTGCCAGAAA 
GCCATGCAGG GCCTGACGGG CCGCAAGTTC GCCAACAGAG TGGTTGTCAC AAAATACTGT 
GACCCCGACT CTTATCACCG CCGGGACTTC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromatin environment, transcriptional regulation, and splicing distinguish lincRNAs and mRNAs.
Genome research27(1)27-37(2017 Jan)
Mel? M,Mattioli K,Mallard W,Shechner DM,Gerhardinger C,Rinn JL

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U2AF2 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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