SLC46A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC46A1
Entrez Gene ID	113235
Full Name	solute carrier family 46 member 1
Synonyms	G21,HCP1,PCFT
General protein information	Preferred Names solute carrier family 46 member 1 Names proton-coupled folate transporter heme carrier protein 1 solute carrier family 46 (folate transporter), member 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q11.2
Summary	This gene encodes a transmembrane proton-coupled folate transporter protein that facilitates the movement of folate and antifolate substrates across cell membranes, optimally in acidic pH environments. This protein is also expressed in the brain and choroid plexus where it transports folates into the central nervous system. This protein further functions as a heme transporter in duodenal enterocytes, and potentially in other tissues like liver and kidney. Its localization to the apical membrane or cytoplasm of intestinal cells is modulated by dietary iron levels. Mutations in this gene are associated with autosomal recessive hereditary folate malabsorption disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013].
Disorder MIM:	611672
Disorder Html:	Folate malabsorption, hereditary, 229050 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017024110.1	XP_016879599.1	proton-coupled folate transporter isoform X2
NM_001242366.2	NP_001229295.1	proton-coupled folate transporter isoform 2
XM_005277786.3	XP_005277843.1	proton-coupled folate transporter isoform X1
NM_080669.6	NP_542400.2	proton-coupled folate transporter isoform 1
NM_001242366.3	NP_001229295.1	proton-coupled folate transporter isoform 2
NM_080669.5	NP_542400.2	proton-coupled folate transporter isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SLC46A1	XP_003435354.1
Caenorhabditis elegans (roundworm)	Y4C6B.5	NP_500781.2
Bos taurus (cattle)	SLC46A1	NP_001073053.1
Gallus gallus (chicken)	SLC46A1	NP_001191995.1
Xenopus tropicalis (tropical clawed frog)	slc46a1	NP_001120610.1
	SLC46A1	XP_001106954.1
Mus musculus (house mouse)	Slc46a1	NP_081016.2
Homo sapiens (human)	SLC46A1	NP_542400.2
Rattus norvegicus (Norway rat)	Slc46a1	NP_001013991.1
Danio rerio (zebrafish)	slc46a1	NP_956579.1
Caenorhabditis elegans (roundworm)	Y43F8A.5	NP_507773.1

Gene Ontology
Bibliography

Related articles in PubMed

Human proton coupled folic acid transporter is a monodisperse oligomer in the lauryl maltose neopentyl glycol solubilized state.
Aduri NG, Ernst HA, Prabhala BK, Bhatt S, Boesen T, Gajhede M, Mirza O
Biochemical and biophysical research communications495(2)1738-1743(2018 Jan)

Residues in the eighth transmembrane domain of the proton-coupled folate transporter (SLC46A1) play an important role in defining the aqueous translocation pathway and in folate substrate binding.
Aluri S, Zhao R, Fiser A, Goldman ID
Biochimica et biophysica acta1859(11)2193-2202(2017 11)

Role of proton-coupled folate transporter in pemetrexed resistance of mesothelioma: clinical evidence and new pharmacological tools.
Giovannetti E, Zucali PA, Assaraf YG, Funel N, Gemelli M, Stark M, Thunnissen E, Hou Z, Muller IB, Struys EA, Perrino M, Jansen G, Matherly LH, Peters GJ
Annals of oncology : official journal of the European Society for Medical Oncology28(11)2725-2732(2017 Nov)

Substituted-cysteine accessibility and cross-linking identify an exofacial cleft in the 7
Aluri S, Zhao R, Fiser A, Goldman ID
American journal of physiology. Cell physiologyajpcell002152017(2017 Nov)

Role of the tryptophan residues in proton-coupled folate transporter (PCFT-SLC46A1) function.
Najmi M, Zhao R, Fiser A, Goldman ID
American journal of physiology. Cell physiology311(1)C150-7(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The 8th transmembrane helix of PCFT plays an important role in defining the aqueous channel and the folate binding pocket.
Title: Residues in the eighth transmembrane domain of the proton-coupled folate transporter (SLC46A1) play an important role in defining the aqueous translocation pathway and in folate substrate binding.

The hydrophobicity of the PCFT W299 residue is important for function suggesting that during the transport cycle this residue interacts with the lipid membrane thereby impacting on the oscillation of the carrier and, indirectly, on the folate binding pocket.
Title: Role of the tryptophan residues in proton-coupled folate transporter (PCFT-SLC46A1) function.

A homology model of PCFT, based upon the Escherichia coliglycerol 3-phosphate transporter structure, predicted that PCFT transmembrane domains (TMDs) 1, 2, 7, and 11 form an extracellular gate in the inward-open conformation.
Title: Identification of an Extracellular Gate for the Proton-coupled Folate Transporter (PCFT-SLC46A1) by Cysteine Cross-linking.

we observed nominally significant (but not definitive) associations between SLC46A1 SNPs and NTDs and oral clefts, and this finding warrants follow-up in other populations.
Title: Evaluation of proton-coupled folate transporter (SLC46A1) polymorphisms as risk factors for neural tube defects and oral clefts.

We conclude that SLC46A1/PCFT and SLC19A1/RFC-1 are associated with DFS of patients with colorectal cancer and hypothesize that poor response to 5-fluorouracil plus leucovorin therapy in some patients may be linked to low expression of these genes.
Title: Expression of Folate Pathway Genes in Stage III Colorectal Cancer Correlates with Recurrence Status Following Adjuvant Bolus 5-FU-Based Chemotherapy.

The following SLC46A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC46A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16708	NM_080669.6 Latest version!	Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu16737	NM_001242366.3 Latest version!	Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu50063	XM_005277786.3 Latest version!	Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu86544	XM_017024110.1 Latest version!	Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu16737	NM_001242366.2	Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu16708	NM_080669.5	Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC46A1 ( NM_080669.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_542400.2 Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16708
Clone ID Related Accession (Same CDS sequence)	NM_080669.6 , NM_080669.5
Accession Version	NM_080669.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	proton-coupled folate transporter isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA188147.1, DB121588.1, AK054669.1, AK074161.1, DA436636.1, DA527125.1, DA013269.1, BC022100.1 and BC065365.1. On Nov 22, 2018 this sequence version replaced NM_080669.5. Transcript Variant: This variant (1, also known as HCP-1A) encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.160177.1, SRR1660807.211236.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000612814.5/ ENSP00000480703.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC 
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC 
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC 
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG 
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC 
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC 
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT 
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG 
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC 
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA 
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC 
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA 
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG 
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC 
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG 
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC 
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA 
GGATATGGGT TGCTTTTCCT GTCATTAGTC ATCACACCTG TCATCCGGGC TAAACTCTCC 
AAGCTGGTGA GAGAGACAGA GCAGGGTGCT CTCTTTTCTG CTGTGGCCTG TGTGAATAGC 
CTGGCCATGC TGACGGCCTC CGGCATCTTC AACTCACTCT ACCCAGCCAC TCTGAACTTT 
ATGAAGGGGT TCCCCTTCCT CCTGGGAGCT GGCCTCCTGC TCATCCCGGC TGTTCTGATT 
GGGATGCTGG AAAAGGCTGA TCCTCACCTC GAGTTCCAGC AGTTTCCCCA GAGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542400.2
CDS	99..1478
Translation	MEGSASPPEKPRARPAAAVLCRGPVEPLVFLANFALVLQGPLTTQYLWHRFSADLGYNGTRQRGGCSNRSADPTMQEVETLTSHWTLYMNVGGFLVGLFSSTLLGAWSDSVGRRPLLVLASLGLLLQALVSVFVVQLQLHVGYFVLGRILCALLGDFGGLLAASFASVADVSSSRSRTFRMALLEASIGVAGMLASLLGGHWLRAQGYANPFWLALALLIAMTLYAAFCFGETLKEPKSTRLFTFRHHRSIVQLYVAPAPEKSRKHLALYSLAIFVVITVHFGAQDILTLYELSTPLCWDSKLIGYGSAAQHLPYLTSLLALKLLQYCLADAWVAEIGLAFNILGMVVFAFATITPLMFTGYGLLFLSLVITPVIRAKLSKLVRETEQGALFSAVACVNSLAMLTASGIFNSLYPATLNFMKGFPFLLGAGLLLIPAVLIGMLEKADPHLEFQQFPQSP

Target ORF information:

RefSeq Version	NM_080669.6
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080669.6

ORF Insert Sequence:

ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC 
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC 
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC 
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG 
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC 
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC 
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT 
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG 
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC 
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA 
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC 
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA 
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG 
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC 
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG 
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC 
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA 
GGATATGGGT TGCTTTTCCT GTCATTAGTC ATCACACCTG TCATCCGGGC TAAACTCTCC 
AAGCTGGTGA GAGAGACAGA GCAGGGTGCT CTCTTTTCTG CTGTGGCCTG TGTGAATAGC 
CTGGCCATGC TGACGGCCTC CGGCATCTTC AACTCACTCT ACCCAGCCAC TCTGAACTTT 
ATGAAGGGGT TCCCCTTCCT CCTGGGAGCT GGCCTCCTGC TCATCCCGGC TGTTCTGATT 
GGGATGCTGG AAAAGGCTGA TCCTCACCTC GAGTTCCAGC AGTTTCCCCA GAGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC46A1 ( NM_080669.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_542400.2 Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16708
Clone ID Related Accession (Same CDS sequence)	NM_080669.6 , NM_080669.5
Accession Version	NM_080669.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	proton-coupled folate transporter isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA188147.1, DB121588.1, AK054669.1, AK074161.1, DA436636.1, DA527125.1, DA013269.1, BC022100.1 and BC065365.1. On Aug 18, 2013 this sequence version replaced NM_080669.4. Transcript Variant: This variant (1, also known as HCP-1A) encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC 
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC 
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC 
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG 
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC 
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC 
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT 
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG 
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC 
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA 
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC 
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA 
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG 
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC 
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG 
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC 
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA 
GGATATGGGT TGCTTTTCCT GTCATTAGTC ATCACACCTG TCATCCGGGC TAAACTCTCC 
AAGCTGGTGA GAGAGACAGA GCAGGGTGCT CTCTTTTCTG CTGTGGCCTG TGTGAATAGC 
CTGGCCATGC TGACGGCCTC CGGCATCTTC AACTCACTCT ACCCAGCCAC TCTGAACTTT 
ATGAAGGGGT TCCCCTTCCT CCTGGGAGCT GGCCTCCTGC TCATCCCGGC TGTTCTGATT 
GGGATGCTGG AAAAGGCTGA TCCTCACCTC GAGTTCCAGC AGTTTCCCCA GAGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542400.2
CDS	99..1478
Translation	MEGSASPPEKPRARPAAAVLCRGPVEPLVFLANFALVLQGPLTTQYLWHRFSADLGYNGTRQRGGCSNRSADPTMQEVETLTSHWTLYMNVGGFLVGLFSSTLLGAWSDSVGRRPLLVLASLGLLLQALVSVFVVQLQLHVGYFVLGRILCALLGDFGGLLAASFASVADVSSSRSRTFRMALLEASIGVAGMLASLLGGHWLRAQGYANPFWLALALLIAMTLYAAFCFGETLKEPKSTRLFTFRHHRSIVQLYVAPAPEKSRKHLALYSLAIFVVITVHFGAQDILTLYELSTPLCWDSKLIGYGSAAQHLPYLTSLLALKLLQYCLADAWVAEIGLAFNILGMVVFAFATITPLMFTGYGLLFLSLVITPVIRAKLSKLVRETEQGALFSAVACVNSLAMLTASGIFNSLYPATLNFMKGFPFLLGAGLLLIPAVLIGMLEKADPHLEFQQFPQSP

Target ORF information:

RefSeq Version	NM_080669.5
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080669.5

ORF Insert Sequence:

ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC 
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC 
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC 
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG 
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC 
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC 
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT 
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG 
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC 
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA 
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC 
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA 
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG 
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC 
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG 
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC 
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA 
GGATATGGGT TGCTTTTCCT GTCATTAGTC ATCACACCTG TCATCCGGGC TAAACTCTCC 
AAGCTGGTGA GAGAGACAGA GCAGGGTGCT CTCTTTTCTG CTGTGGCCTG TGTGAATAGC 
CTGGCCATGC TGACGGCCTC CGGCATCTTC AACTCACTCT ACCCAGCCAC TCTGAACTTT 
ATGAAGGGGT TCCCCTTCCT CCTGGGAGCT GGCCTCCTGC TCATCCCGGC TGTTCTGATT 
GGGATGCTGG AAAAGGCTGA TCCTCACCTC GAGTTCCAGC AGTTTCCCCA GAGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC46A1 ( NM_001242366.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229295.1 Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16737
Clone ID Related Accession (Same CDS sequence)	NM_001242366.2 , NM_001242366.3
Accession Version	NM_001242366.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	proton-coupled folate transporter isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA188147.1, BC010691.1, AK074161.1, DA436636.1, DA527125.1, DA013269.1, BC022100.1 and BC065365.1. On Aug 18, 2013 this sequence version replaced NM_001242366.1. Transcript Variant: This variant (2, also known as HCP-1B) lacks an in-frame coding exon compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment (containing one of the transmembrane domains) compared to isoform 1. This isoform was shown to have a different subcellular localization compared to the longer isoform 1 (PMID:17947394). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.139018.1, BC010691.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC 
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC 
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC 
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG 
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC 
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC 
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT 
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG 
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC 
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA 
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC 
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA 
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG 
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC 
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG 
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC 
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA 
GGTGCTCTCT TTTCTGCTGT GGCCTGTGTG AATAGCCTGG CCATGCTGAC GGCCTCCGGC 
ATCTTCAACT CACTCTACCC AGCCACTCTG AACTTTATGA AGGGGTTCCC CTTCCTCCTG 
GGAGCTGGCC TCCTGCTCAT CCCGGCTGTT CTGATTGGGA TGCTGGAAAA GGCTGATCCT 
CACCTCGAGT TCCAGCAGTT TCCCCAGAGC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229295.1
CDS	99..1394
Translation	MEGSASPPEKPRARPAAAVLCRGPVEPLVFLANFALVLQGPLTTQYLWHRFSADLGYNGTRQRGGCSNRSADPTMQEVETLTSHWTLYMNVGGFLVGLFSSTLLGAWSDSVGRRPLLVLASLGLLLQALVSVFVVQLQLHVGYFVLGRILCALLGDFGGLLAASFASVADVSSSRSRTFRMALLEASIGVAGMLASLLGGHWLRAQGYANPFWLALALLIAMTLYAAFCFGETLKEPKSTRLFTFRHHRSIVQLYVAPAPEKSRKHLALYSLAIFVVITVHFGAQDILTLYELSTPLCWDSKLIGYGSAAQHLPYLTSLLALKLLQYCLADAWVAEIGLAFNILGMVVFAFATITPLMFTGALFSAVACVNSLAMLTASGIFNSLYPATLNFMKGFPFLLGAGLLLIPAVLIGMLEKADPHLEFQQFPQSP

Target ORF information:

RefSeq Version	NM_001242366.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242366.2

ORF Insert Sequence:

ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC 
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC 
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC 
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG 
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC 
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC 
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT 
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG 
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC 
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA 
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC 
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA 
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG 
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC 
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG 
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC 
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA 
GGTGCTCTCT TTTCTGCTGT GGCCTGTGTG AATAGCCTGG CCATGCTGAC GGCCTCCGGC 
ATCTTCAACT CACTCTACCC AGCCACTCTG AACTTTATGA AGGGGTTCCC CTTCCTCCTG 
GGAGCTGGCC TCCTGCTCAT CCCGGCTGTT CTGATTGGGA TGCTGGAAAA GGCTGATCCT 
CACCTCGAGT TCCAGCAGTT TCCCCAGAGC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC46A1 ( NM_001242366.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229295.1 Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant 2, mRNA.

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CloneID	OHu16737
Clone ID Related Accession (Same CDS sequence)	NM_001242366.2 , NM_001242366.3
Accession Version	NM_001242366.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	proton-coupled folate transporter isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA188147.1, BC010691.1, AK074161.1, DA436636.1, DA527125.1, DA013269.1, BC022100.1 and BC065365.1. On May 31, 2019 this sequence version replaced NM_001242366.2. Transcript Variant: This variant (2, also known as HCP-1B) lacks an in-frame coding exon compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment (containing one of the transmembrane domains) compared to isoform 1. This isoform was shown to have a different subcellular localization compared to the longer isoform 1 (PMID:17947394). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.139018.1, SRR7346977.2885715.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC 
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC 
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC 
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG 
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC 
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC 
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT 
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG 
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC 
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA 
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC 
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA 
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG 
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC 
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG 
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC 
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA 
GGTGCTCTCT TTTCTGCTGT GGCCTGTGTG AATAGCCTGG CCATGCTGAC GGCCTCCGGC 
ATCTTCAACT CACTCTACCC AGCCACTCTG AACTTTATGA AGGGGTTCCC CTTCCTCCTG 
GGAGCTGGCC TCCTGCTCAT CCCGGCTGTT CTGATTGGGA TGCTGGAAAA GGCTGATCCT 
CACCTCGAGT TCCAGCAGTT TCCCCAGAGC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229295.1
CDS	99..1394
Translation	MEGSASPPEKPRARPAAAVLCRGPVEPLVFLANFALVLQGPLTTQYLWHRFSADLGYNGTRQRGGCSNRSADPTMQEVETLTSHWTLYMNVGGFLVGLFSSTLLGAWSDSVGRRPLLVLASLGLLLQALVSVFVVQLQLHVGYFVLGRILCALLGDFGGLLAASFASVADVSSSRSRTFRMALLEASIGVAGMLASLLGGHWLRAQGYANPFWLALALLIAMTLYAAFCFGETLKEPKSTRLFTFRHHRSIVQLYVAPAPEKSRKHLALYSLAIFVVITVHFGAQDILTLYELSTPLCWDSKLIGYGSAAQHLPYLTSLLALKLLQYCLADAWVAEIGLAFNILGMVVFAFATITPLMFTGALFSAVACVNSLAMLTASGIFNSLYPATLNFMKGFPFLLGAGLLLIPAVLIGMLEKADPHLEFQQFPQSP

Target ORF information:

RefSeq Version	NM_001242366.3
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242366.3

ORF Insert Sequence:

ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC 
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC 
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC 
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG 
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC 
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC 
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT 
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG 
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC 
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA 
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC 
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA 
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG 
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC 
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG 
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC 
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA 
GGTGCTCTCT TTTCTGCTGT GGCCTGTGTG AATAGCCTGG CCATGCTGAC GGCCTCCGGC 
ATCTTCAACT CACTCTACCC AGCCACTCTG AACTTTATGA AGGGGTTCCC CTTCCTCCTG 
GGAGCTGGCC TCCTGCTCAT CCCGGCTGTT CTGATTGGGA TGCTGGAAAA GGCTGATCCT 
CACCTCGAGT TCCAGCAGTT TCCCCAGAGC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC46A1 ( XM_005277786.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005277843.1 Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant X1, mRNA.

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CloneID	OHu50063
Clone ID Related Accession (Same CDS sequence)	XM_005277786.3
Accession Version	XM_005277786.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	proton-coupled folate transporter isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005277786.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC 
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC 
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC 
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG 
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC 
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC 
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT 
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG 
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC 
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA 
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC 
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA 
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG 
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC 
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG 
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC 
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA 
GGATGCTGGA AAAGGCTGAT CCTCACCTCG AGTTCCAGCA GTTTCCCCAG AGCCCCTGAT 
CTGCCTGGAC CAGAAGACAG AGGGCAAGAG GAGCAAAGTG AACACCAAGC AACTGGAGGT 
CTGCAGCTGG AAGCCCAGCC CACAGCAGGA CAAGCAACTC TTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005277843.1
CDS	99..1346
Translation	MEGSASPPEKPRARPAAAVLCRGPVEPLVFLANFALVLQGPLTTQYLWHRFSADLGYNGTRQRGGCSNRSADPTMQEVETLTSHWTLYMNVGGFLVGLFSSTLLGAWSDSVGRRPLLVLASLGLLLQALVSVFVVQLQLHVGYFVLGRILCALLGDFGGLLAASFASVADVSSSRSRTFRMALLEASIGVAGMLASLLGGHWLRAQGYANPFWLALALLIAMTLYAAFCFGETLKEPKSTRLFTFRHHRSIVQLYVAPAPEKSRKHLALYSLAIFVVITVHFGAQDILTLYELSTPLCWDSKLIGYGSAAQHLPYLTSLLALKLLQYCLADAWVAEIGLAFNILGMVVFAFATITPLMFTGCWKRLILTSSSSSFPRAPDLPGPEDRGQEEQSEHQATGGLQLEAQPTAGQATLV

Target ORF information:

RefSeq Version	XM_005277786.3
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005277786.3

ORF Insert Sequence:

ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC 
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC 
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC 
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG 
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC 
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC 
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT 
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG 
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC 
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA 
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC 
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA 
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG 
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC 
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG 
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC 
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA 
GGATGCTGGA AAAGGCTGAT CCTCACCTCG AGTTCCAGCA GTTTCCCCAG AGCCCCTGAT 
CTGCCTGGAC CAGAAGACAG AGGGCAAGAG GAGCAAAGTG AACACCAAGC AACTGGAGGT 
CTGCAGCTGG AAGCCCAGCC CACAGCAGGA CAAGCAACTC TTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC46A1 ( XM_017024110.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879599.1 Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant X2, mRNA.

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CloneID	OHu86544
Clone ID Related Accession (Same CDS sequence)	XM_017024110.1
Accession Version	XM_017024110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	proton-coupled folate transporter isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAG TGGAGACCCT TACCTCCCAC TGGACCCTCT ACATGAACGT GGGCGGCTTC 
CTGGTGGGGC TCTTCTCGTC CACCCTGCTG GGAGCTTGGA GCGACAGTGT GGGCCGCCGC 
CCGCTGCTAG TGCTGGCCTC GCTGGGCCTG CTGCTCCAGG CCCTAGTGTC CGTTTTTGTG 
GTGCAGCTGC AGCTCCACGT CGGCTACTTC GTGCTGGGTC GCATCCTTTG TGCCCTCCTC 
GGCGACTTCG GTGGCCTTCT GGCTGCTAGC TTTGCGTCCG TGGCAGATGT CAGCTCCAGT 
CGCAGCCGCA CCTTCCGGAT GGCCCTGCTG GAAGCCAGCA TCGGGGTGGC TGGGATGCTG 
GCAAGCCTCC TCGGGGGCCA CTGGCTCCGG GCCCAGGGTT ATGCCAACCC CTTCTGGCTG 
GCCTTGGCCT TGCTGATAGC CATGACTCTC TATGCAGCTT TCTGCTTTGG TGAGACCTTA 
AAGGAGCCAA AGTCCACCCG GCTCTTCACG TTCCGTCACC ACCGATCCAT TGTCCAGCTC 
TATGTGGCTC CCGCCCCAGA GAAGTCCAGG AAACATTTAG CCCTCTACTC ACTGGCCATC 
TTCGTGGTGA TCACTGTGCA CTTTGGGGCC CAGGACATCT TAACCCTTTA TGAACTAAGC 
ACACCCCTCT GCTGGGACTC CAAACTAATC GGCTATGGTT CTGCAGCTCA GCATCTCCCC 
TACCTCACCA GCCTGCTGGC CCTGAAGCTC CTGCAGTACT GCCTGGCCGA TGCCTGGGTA 
GCTGAGATCG GCCTGGCCTT CAACATCCTG GGGATGGTGG TCTTTGCCTT TGCCACTATC 
ACGCCTCTCA TGTTCACAGG ATATGGGTTG CTTTTCCTGT CATTAGTCAT CACACCTGTC 
ATCCGGGCTA AACTCTCCAA GCTGGTGAGA GAGACAGAGC AGGGTGCTCT CTTTTCTGCT 
GTGGCCTGTG TGAATAGCCT GGCCATGCTG ACGGCCTCCG GCATCTTCAA CTCACTCTAC 
CCAGCCACTC TGAACTTTAT GAAGGGGTTC CCCTTCCTCC TGGGAGCTGG CCTCCTGCTC 
ATCCCGGCTG TTCTGATTGG GATGCTGGAA AAGGCTGATC CTCACCTCGA GTTCCAGCAG 
TTTCCCCAGA GCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879599.1
CDS	280..1437
Translation	MQEVETLTSHWTLYMNVGGFLVGLFSSTLLGAWSDSVGRRPLLVLASLGLLLQALVSVFVVQLQLHVGYFVLGRILCALLGDFGGLLAASFASVADVSSSRSRTFRMALLEASIGVAGMLASLLGGHWLRAQGYANPFWLALALLIAMTLYAAFCFGETLKEPKSTRLFTFRHHRSIVQLYVAPAPEKSRKHLALYSLAIFVVITVHFGAQDILTLYELSTPLCWDSKLIGYGSAAQHLPYLTSLLALKLLQYCLADAWVAEIGLAFNILGMVVFAFATITPLMFTGYGLLFLSLVITPVIRAKLSKLVRETEQGALFSAVACVNSLAMLTASGIFNSLYPATLNFMKGFPFLLGAGLLLIPAVLIGMLEKADPHLEFQQFPQSP

Target ORF information:

RefSeq Version	XM_017024110.1
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 46 member 1 (SLC46A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024110.1

ORF Insert Sequence:

ATGCAGGAAG TGGAGACCCT TACCTCCCAC TGGACCCTCT ACATGAACGT GGGCGGCTTC 
CTGGTGGGGC TCTTCTCGTC CACCCTGCTG GGAGCTTGGA GCGACAGTGT GGGCCGCCGC 
CCGCTGCTAG TGCTGGCCTC GCTGGGCCTG CTGCTCCAGG CCCTAGTGTC CGTTTTTGTG 
GTGCAGCTGC AGCTCCACGT CGGCTACTTC GTGCTGGGTC GCATCCTTTG TGCCCTCCTC 
GGCGACTTCG GTGGCCTTCT GGCTGCTAGC TTTGCGTCCG TGGCAGATGT CAGCTCCAGT 
CGCAGCCGCA CCTTCCGGAT GGCCCTGCTG GAAGCCAGCA TCGGGGTGGC TGGGATGCTG 
GCAAGCCTCC TCGGGGGCCA CTGGCTCCGG GCCCAGGGTT ATGCCAACCC CTTCTGGCTG 
GCCTTGGCCT TGCTGATAGC CATGACTCTC TATGCAGCTT TCTGCTTTGG TGAGACCTTA 
AAGGAGCCAA AGTCCACCCG GCTCTTCACG TTCCGTCACC ACCGATCCAT TGTCCAGCTC 
TATGTGGCTC CCGCCCCAGA GAAGTCCAGG AAACATTTAG CCCTCTACTC ACTGGCCATC 
TTCGTGGTGA TCACTGTGCA CTTTGGGGCC CAGGACATCT TAACCCTTTA TGAACTAAGC 
ACACCCCTCT GCTGGGACTC CAAACTAATC GGCTATGGTT CTGCAGCTCA GCATCTCCCC 
TACCTCACCA GCCTGCTGGC CCTGAAGCTC CTGCAGTACT GCCTGGCCGA TGCCTGGGTA 
GCTGAGATCG GCCTGGCCTT CAACATCCTG GGGATGGTGG TCTTTGCCTT TGCCACTATC 
ACGCCTCTCA TGTTCACAGG ATATGGGTTG CTTTTCCTGT CATTAGTCAT CACACCTGTC 
ATCCGGGCTA AACTCTCCAA GCTGGTGAGA GAGACAGAGC AGGGTGCTCT CTTTTCTGCT 
GTGGCCTGTG TGAATAGCCT GGCCATGCTG ACGGCCTCCG GCATCTTCAA CTCACTCTAC 
CCAGCCACTC TGAACTTTAT GAAGGGGTTC CCCTTCCTCC TGGGAGCTGG CCTCCTGCTC 
ATCCCGGCTG TTCTGATTGG GATGCTGGAA AAGGCTGATC CTCACCTCGA GTTCCAGCAG 
TTTCCCCAGA GCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human proton coupled folic acid transporter is a monodisperse oligomer in the lauryl maltose neopentyl glycol solubilized state.
Biochemical and biophysical research communications495(2)1738-1743(2018 Jan)
Aduri NG,Ernst HA,Prabhala BK,Bhatt S,Boesen T,Gajhede M,Mirza O

Residues in the eighth transmembrane domain of the proton-coupled folate transporter (SLC46A1) play an important role in defining the aqueous translocation pathway and in folate substrate binding.
Biochimica et biophysica acta1859(11)2193-2202(2017 11)
Aluri S,Zhao R,Fiser A,Goldman ID

Role of proton-coupled folate transporter in pemetrexed resistance of mesothelioma: clinical evidence and new pharmacological tools.
Annals of oncology : official journal of the European Society for Medical Oncology28(11)2725-2732(2017 Nov)
Giovannetti E,Zucali PA,Assaraf YG,Funel N,Gemelli M,Stark M,Thunnissen E,Hou Z,Muller IB,Struys EA,Perrino M,Jansen G,Matherly LH,Peters GJ

Substituted-cysteine accessibility and cross-linking identify an exofacial cleft in the 7
American journal of physiology. Cell physiologyajpcell002152017(2017 Nov)
Aluri S,Zhao R,Fiser A,Goldman ID

Role of the tryptophan residues in proton-coupled folate transporter (PCFT-SLC46A1) function.
American journal of physiology. Cell physiology311(1)C150-7(2016 Jul)
Najmi M,Zhao R,Fiser A,Goldman ID

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SLC46A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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